asns cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asns
Entrez Gene ID	394138
Full Name	asparagine synthetase
Synonyms	zgc:56605,zgc:76901
General protein information	Preferred Names asparagine synthetase Names asparagine synthetase [glutamine-hydrolyzing]
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201163.3	NP_957457.3	asparagine synthetase [glutamine-hydrolyzing]

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC475240	XP_005628593.1
Caenorhabditis elegans (roundworm)	asns-1	NP_505204.1
	ASNS	XP_001089407.1
Bos taurus (cattle)	ASNS	NP_001069121.1
Rattus norvegicus (Norway rat)	Asns	NP_037211.2
Danio rerio (zebrafish)	asns	NP_957457.2
Pan troglodytes (chimpanzee)	ASNS	XP_001171342.1
Gallus gallus (chicken)	ASNS	NP_001026148.1
Xenopus tropicalis (tropical clawed frog)	asns	NP_001005721.1
Mus musculus (house mouse)	Asns	NP_036185.1
Homo sapiens (human)	ASNS	NP_899199.2

Gene Ontology
Bibliography

Related articles in PubMed

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Li JM, Li LY, Qin X, Ning LJ, Lu DL, Li DL, Zhang ML, Wang X, Du ZY
Scientific reports740815(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following asns gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asns cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53286	NM_201163.3 Latest version!	Danio rerio asparagine synthetase (asns), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asns ( NM_201163.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957457.3 Danio rerio asparagine synthetase (asns), mRNA.

Price & Availability↑

CloneID	ODa53286
Clone ID Related Accession (Same CDS sequence)	NM_201163.3
Accession Version	NM_201163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	asparagine synthetase [glutamine-hydrolyzing]
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX842688.12. On Feb 19, 2016 this sequence version replaced NM_201163.2. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments, RNA-seq data and orthologous alignments. ##Evidence-Data-START## Transcript exon combination :: BC067140.1, GFIL01001693.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGGCA TATGGGCCTT GTTTGGTAGT GATGAGTGCC TTTCAGTTCA GTGCACTAAT 
GCCATGAAGA TCTTTCATCG TGGACCGGAT GCTTTCCGCT TCGAGAATGT CAACGGTTTC 
ACTAACTGCT GCTTTGGGTT TCACCGTCTT GCGATTGTGG ACCAGCTTTA TGGCATGCAG 
CCTCTGCGAG TCAAAAAGTT CCCTTATCTG TGGTTATGTT ACAACGGTGA AATCTACAAC 
CACATCAAGC TGAAGAATCA CTTTGAGTTT GACTATCAAA CCAAAGTAGA TGGTGAGATC 
CTTCTGCATC TGTACGATCG CTTTGGAATC GAGAAGATGT GCTCCCTGCT GGATGGTGTG 
TTCGCCTTCA TCTTATTAGA CACAGCTAAC AGAAAGGTTC ACTTGGGAAG AGATACATAC 
GGCGTGAGAC CGCTATTCAG AATGCTGACT GACGATGGTT TTCTAGCTGT CTGTTCTGAA 
GGCAAAGGTC TAACACAGAT CAAACACTCC ATGCCCACTG CAAAGATCAC TGCTTTTCCA 
CCGGGACACT TTGAGGTGTT TGACTTGAAG CTCAATGGGA AAGTGGAGTC TGTTCAGATG 
GATCGCTTTC ACTGCTGCAC AGACAAACCC AAACATGCTG ACTTTAACAA ACTCGAGGGA 
CTGGGCACAG ACTTCGAATT GGAGACAGTC AAGAGCAACA TTAGGATCCT GTTTGAGGAT 
GCAGTAAGGA AACGCTTGAT GGCTCACAGA AGAATCGGTT GCCTTCTCTC AGGTGGTCTC 
GACTCAAGTC TGGTTGCTGC TTTGCTTGTG AAACTGGCCA AAGAAGAGAA GCTTCCATAT 
CCCATTCAGA CTTTTTCCAT TGGTGCTGAG GACAGTCCAG ATGTCGCTGC TGCTCGCAAG 
GTGGCAGACT ACATTGGCAG CGAGCACCAT GTGGTAAACT TTACACCAGA GGAAGGCATC 
AGTGCGCTGG AGGACGTCAT CGTTCACTTA GAGAGTTATG ACATCACCAC TGTTCGTGCC 
TCTATTGGAA TGTACCTGGT ATCAAAATAC ATCCGAGAGA AGACAGACAG TGTGGTGATT 
TTCTCAGGTG AAGGTTCAGA TGAGCTGACA CAAGGATACA TCTACTTTCA TAAGGCTCCA 
TCACCTAAAG CTGGTGCAGA GGACAGTGTG CGTCTGCTGG AAGAGCTTTA TCTGTTCGAT 
GTGCTGAGAG CCGACAGAAC CACTGCTGCT CATGGACTGG AGTTGAGAGT GCCTTTCCTG 
GACCACAGGT TCACAGCGTA CTACCTCTCT CTGCCTGAAG AGATGAGAGT GCCTAAGAAT 
GGTGTGGAGA AGCATCTTTT GAGGGATGCA TTTGATGGGA TGAACCTGAT TCCTGATGAG 
ATCCTTTGGA GAAGGAAAGA GGCTTTCAGT GACGGTTTGA CATCAGTGAA GAAATCGTGG 
TACACCAGCC TACAGGAGCA CATCGAGTCT GAGCTGAATG ACTCTCAGTT GGAGAATGCT 
AATAAACTGT ACCATATCAA TCCTCCCACA ACCAAAGAGG GCTTGTTCAT CAGACAGATC 
TTTGAGAAGC ATTACCCTGG CCAAGGTGAG TGGACGCCAC ATTACTGGAT GCCCCGCTGG 
ATCAAGGCCA CAGACCCTTC AGCCAGAACA CTGTCCATCT ACAAGCCTGA TAAAGAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957457.3
CDS	74..1756
Translation	MCGIWALFGSDECLSVQCTNAMKIFHRGPDAFRFENVNGFTNCCFGFHRLAIVDQLYGMQPLRVKKFPYLWLCYNGEIYNHIKLKNHFEFDYQTKVDGEILLHLYDRFGIEKMCSLLDGVFAFILLDTANRKVHLGRDTYGVRPLFRMLTDDGFLAVCSEGKGLTQIKHSMPTAKITAFPPGHFEVFDLKLNGKVESVQMDRFHCCTDKPKHADFNKLEGLGTDFELETVKSNIRILFEDAVRKRLMAHRRIGCLLSGGLDSSLVAALLVKLAKEEKLPYPIQTFSIGAEDSPDVAAARKVADYIGSEHHVVNFTPEEGISALEDVIVHLESYDITTVRASIGMYLVSKYIREKTDSVVIFSGEGSDELTQGYIYFHKAPSPKAGAEDSVRLLEELYLFDVLRADRTTAAHGLELRVPFLDHRFTAYYLSLPEEMRVPKNGVEKHLLRDAFDGMNLIPDEILWRRKEAFSDGLTSVKKSWYTSLQEHIESELNDSQLENANKLYHINPPTTKEGLFIRQIFEKHYPGQGEWTPHYWMPRWIKATDPSARTLSIYKPDKEQ

Target ORF information:

RefSeq Version	NM_201163.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio asparagine synthetase (asns), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201163.3

ORF Insert Sequence:

ATGTGTGGCA TATGGGCCTT GTTTGGTAGT GATGAGTGCC TTTCAGTTCA GTGCACTAAT 
GCCATGAAGA TCTTTCATCG TGGACCGGAT GCTTTCCGCT TCGAGAATGT CAACGGTTTC 
ACTAACTGCT GCTTTGGGTT TCACCGTCTT GCGATTGTGG ACCAGCTTTA TGGCATGCAG 
CCTCTGCGAG TCAAAAAGTT CCCTTATCTG TGGTTATGTT ACAACGGTGA AATCTACAAC 
CACATCAAGC TGAAGAATCA CTTTGAGTTT GACTATCAAA CCAAAGTAGA TGGTGAGATC 
CTTCTGCATC TGTACGATCG CTTTGGAATC GAGAAGATGT GCTCCCTGCT GGATGGTGTG 
TTCGCCTTCA TCTTATTAGA CACAGCTAAC AGAAAGGTTC ACTTGGGAAG AGATACATAC 
GGCGTGAGAC CGCTATTCAG AATGCTGACT GACGATGGTT TTCTAGCTGT CTGTTCTGAA 
GGCAAAGGTC TAACACAGAT CAAACACTCC ATGCCCACTG CAAAGATCAC TGCTTTTCCA 
CCGGGACACT TTGAGGTGTT TGACTTGAAG CTCAATGGGA AAGTGGAGTC TGTTCAGATG 
GATCGCTTTC ACTGCTGCAC AGACAAACCC AAACATGCTG ACTTTAACAA ACTCGAGGGA 
CTGGGCACAG ACTTCGAATT GGAGACAGTC AAGAGCAACA TTAGGATCCT GTTTGAGGAT 
GCAGTAAGGA AACGCTTGAT GGCTCACAGA AGAATCGGTT GCCTTCTCTC AGGTGGTCTC 
GACTCAAGTC TGGTTGCTGC TTTGCTTGTG AAACTGGCCA AAGAAGAGAA GCTTCCATAT 
CCCATTCAGA CTTTTTCCAT TGGTGCTGAG GACAGTCCAG ATGTCGCTGC TGCTCGCAAG 
GTGGCAGACT ACATTGGCAG CGAGCACCAT GTGGTAAACT TTACACCAGA GGAAGGCATC 
AGTGCGCTGG AGGACGTCAT CGTTCACTTA GAGAGTTATG ACATCACCAC TGTTCGTGCC 
TCTATTGGAA TGTACCTGGT ATCAAAATAC ATCCGAGAGA AGACAGACAG TGTGGTGATT 
TTCTCAGGTG AAGGTTCAGA TGAGCTGACA CAAGGATACA TCTACTTTCA TAAGGCTCCA 
TCACCTAAAG CTGGTGCAGA GGACAGTGTG CGTCTGCTGG AAGAGCTTTA TCTGTTCGAT 
GTGCTGAGAG CCGACAGAAC CACTGCTGCT CATGGACTGG AGTTGAGAGT GCCTTTCCTG 
GACCACAGGT TCACAGCGTA CTACCTCTCT CTGCCTGAAG AGATGAGAGT GCCTAAGAAT 
GGTGTGGAGA AGCATCTTTT GAGGGATGCA TTTGATGGGA TGAACCTGAT TCCTGATGAG 
ATCCTTTGGA GAAGGAAAGA GGCTTTCAGT GACGGTTTGA CATCAGTGAA GAAATCGTGG 
TACACCAGCC TACAGGAGCA CATCGAGTCT GAGCTGAATG ACTCTCAGTT GGAGAATGCT 
AATAAACTGT ACCATATCAA TCCTCCCACA ACCAAAGAGG GCTTGTTCAT CAGACAGATC 
TTTGAGAAGC ATTACCCTGG CCAAGGTGAG TGGACGCCAC ATTACTGGAT GCCCCGCTGG 
ATCAAGGCCA CAGACCCTTC AGCCAGAACA CTGTCCATCT ACAAGCCTGA TAAAGAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Scientific reports740815(2017 Jan)
Li JM,Li LY,Qin X,Ning LJ,Lu DL,Li DL,Zhang ML,Wang X,Du ZY

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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asns cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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