ORF » Species Summary » Danio rerio » lin28a cDNA ORF clone

lin28a cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following lin28a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lin28a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa38593 NM_201091.1
Latest version!		 Danio rerio lin-28 homolog A (C. elegans) (lin28a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa24010 XM_005159535.4
Latest version!		 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa24011 XM_009294134.3
Latest version!		 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
View Old Accession Versions >>
ODa24010 XM_005159535.3 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa24011 XM_009294134.2 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    lin28a ( NM_201091.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957385.1 Danio rerio lin-28 homolog A (C. elegans) (lin28a), mRNA.
    Price & Availability↑
    CloneID ODa38593
    Clone ID Related Accession (Same CDS sequence) NM_201091.1
    Accession Version NM_201091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Danio rerio(zebrafish)
    Product protein lin-28 homolog A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044433.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044433.1, EH547049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898399, SAMEA898400 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCCCCCGG CAAATCCGCA TCTCAATCAC ACAGGTGGAT GTACAAAGAC AGAGGAGGAA 
    GAAGCCGCGA GCTCCGAGGA AGACTCAGGC TCTTTTCACG GCTCTGGGGT CTGCAAATGG 
    TTTAACGTGC GGATGGGCTT CGGATTTCTG TCCATGACCC ACCGGGAGGG CATCTGTCTG 
    GACTCACCCG TGGATGTCTT CGTCCATCAG AGCAAGCTTC ATATGGAGGG TTTTCGCAGT 
    CTGAAGGAAG GAGAAGCAGT GGAATTCACC TTTAAAAGAT CCTCTAAGGG TCTGGAGTCC 
    TTACAAGTGA CGGGGCCTGG AGGGGCACCA TGTGTGGGCA GCGAGAAGAA ACCTAAGGGT 
    ACCCAAAAGA GGCGGTCAAA AGGAGATCGA TGCTTCAACT GTGGAGGACC CAATCATCAT 
    GCCAAAGAAT GCCAGCTGCC ACCTCAGCCG AAAAAGTGCC ATTTTTGCCA AAGCATCTCC 
    CACATGGTGG CCAACTGCCC AATCAAAGCA CAGCAGTTGT CACCAGGATC TCAGGGCAAA 
    TCGACAACCT CAACAGGAGA AGAGGAGGAC ATGAGCCACA CTCCCTTACT GCCAGAGAGC 
    ACTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_957385.1
    CDS157..765
    Translation

    Target ORF information:

    RefSeq Version NM_201091.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lin-28 homolog A (C. elegans) (lin28a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_201091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCCCCCGG CAAATCCGCA TCTCAATCAC ACAGGTGGAT GTACAAAGAC AGAGGAGGAA 
    GAAGCCGCGA GCTCCGAGGA AGACTCAGGC TCTTTTCACG GCTCTGGGGT CTGCAAATGG 
    TTTAACGTGC GGATGGGCTT CGGATTTCTG TCCATGACCC ACCGGGAGGG CATCTGTCTG 
    GACTCACCCG TGGATGTCTT CGTCCATCAG AGCAAGCTTC ATATGGAGGG TTTTCGCAGT 
    CTGAAGGAAG GAGAAGCAGT GGAATTCACC TTTAAAAGAT CCTCTAAGGG TCTGGAGTCC 
    TTACAAGTGA CGGGGCCTGG AGGGGCACCA TGTGTGGGCA GCGAGAAGAA ACCTAAGGGT 
    ACCCAAAAGA GGCGGTCAAA AGGAGATCGA TGCTTCAACT GTGGAGGACC CAATCATCAT 
    GCCAAAGAAT GCCAGCTGCC ACCTCAGCCG AAAAAGTGCC ATTTTTGCCA AAGCATCTCC 
    CACATGGTGG CCAACTGCCC AATCAAAGCA CAGCAGTTGT CACCAGGATC TCAGGGCAAA 
    TCGACAACCT CAACAGGAGA AGAGGAGGAC ATGAGCCACA CTCCCTTACT GCCAGAGAGC 
    ACTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    lin28a ( XM_005159535.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159592.1 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa24010
    Clone ID Related Accession (Same CDS sequence) XM_005159535.3 , XM_005159535.4
    Accession Version XM_005159535.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein lin-28 homolog A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCGAGA TGGGTGAAAT GGGCTCAGTT TATGAGCAAT ATTACACAGG TGGATGTACA 
    AAGACAGAGG AGGAAGAAGC CGCGAGCTCC GAGGAAGACT CAGGCTCTTT TCACGGCTCT 
    GGGGTCTGCA AATGGTTTAA CGTGCGGATG GGCTTCGGAT TTCTGTCCAT GACCCACCGG 
    GAGGGCATCT GTCTGGACTC ACCCGTGGAT GTCTTCGTCC ATCAGAGCAA GCTTCATATG 
    GAGGGTTTTC GCAGTCTGAA GGAAGGAGAA GCAGTGGAAT TCACCTTTAA AAGATCCTCT 
    AAGGGTCTGG AGTCCTTACA AGTGACGGGG CCTGGAGGGG CACCATGTGT GGGCAGCGAG 
    AAGAAACCTA AGGGTACCCA AAAGAGGCGG TCAAAAGGAG ATCGATGCTT CAACTGTGGA 
    GGACCCAATC ATCATGCCAA AGAATGCCAG CTGCCACCTC AGCCGAAAAA GTGCCATTTT 
    TGCCAAAGCA TCTCCCACAT GGTGGCCAAC TGCCCAATCA AAGCACAGCA GTTGTCACCA 
    GGATCTCAGG GCAAATCGAC AACCTCAACA GGAGAAGAGG AGGACATGAG CCACACTCCC 
    TTACTGCCAG AGAGCACTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159592.1
    CDS254..877
    Translation

    Target ORF information:

    RefSeq Version XM_005159535.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005159535.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCGAGA TGGGTGAAAT GGGCTCAGTT TATGAGCAAT ATTACACAGG TGGATGTACA 
    AAGACAGAGG AGGAAGAAGC CGCGAGCTCC GAGGAAGACT CAGGCTCTTT TCACGGCTCT 
    GGGGTCTGCA AATGGTTTAA CGTGCGGATG GGCTTCGGAT TTCTGTCCAT GACCCACCGG 
    GAGGGCATCT GTCTGGACTC ACCCGTGGAT GTCTTCGTCC ATCAGAGCAA GCTTCATATG 
    GAGGGTTTTC GCAGTCTGAA GGAAGGAGAA GCAGTGGAAT TCACCTTTAA AAGATCCTCT 
    AAGGGTCTGG AGTCCTTACA AGTGACGGGG CCTGGAGGGG CACCATGTGT GGGCAGCGAG 
    AAGAAACCTA AGGGTACCCA AAAGAGGCGG TCAAAAGGAG ATCGATGCTT CAACTGTGGA 
    GGACCCAATC ATCATGCCAA AGAATGCCAG CTGCCACCTC AGCCGAAAAA GTGCCATTTT 
    TGCCAAAGCA TCTCCCACAT GGTGGCCAAC TGCCCAATCA AAGCACAGCA GTTGTCACCA 
    GGATCTCAGG GCAAATCGAC AACCTCAACA GGAGAAGAGG AGGACATGAG CCACACTCCC 
    TTACTGCCAG AGAGCACTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    lin28a ( XM_005159535.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159592.1 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa24010
    Clone ID Related Accession (Same CDS sequence) XM_005159535.3 , XM_005159535.4
    Accession Version XM_005159535.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein lin-28 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCGAGA TGGGTGAAAT GGGCTCAGTT TATGAGCAAT ATTACACAGG TGGATGTACA 
    AAGACAGAGG AGGAAGAAGC CGCGAGCTCC GAGGAAGACT CAGGCTCTTT TCACGGCTCT 
    GGGGTCTGCA AATGGTTTAA CGTGCGGATG GGCTTCGGAT TTCTGTCCAT GACCCACCGG 
    GAGGGCATCT GTCTGGACTC ACCCGTGGAT GTCTTCGTCC ATCAGAGCAA GCTTCATATG 
    GAGGGTTTTC GCAGTCTGAA GGAAGGAGAA GCAGTGGAAT TCACCTTTAA AAGATCCTCT 
    AAGGGTCTGG AGTCCTTACA AGTGACGGGG CCTGGAGGGG CACCATGTGT GGGCAGCGAG 
    AAGAAACCTA AGGGTACCCA AAAGAGGCGG TCAAAAGGAG ATCGATGCTT CAACTGTGGA 
    GGACCCAATC ATCATGCCAA AGAATGCCAG CTGCCACCTC AGCCGAAAAA GTGCCATTTT 
    TGCCAAAGCA TCTCCCACAT GGTGGCCAAC TGCCCAATCA AAGCACAGCA GTTGTCACCA 
    GGATCTCAGG GCAAATCGAC AACCTCAACA GGAGAAGAGG AGGACATGAG CCACACTCCC 
    TTACTGCCAG AGAGCACTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159592.1
    CDS246..869
    Translation

    Target ORF information:

    RefSeq Version XM_005159535.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005159535.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCGAGA TGGGTGAAAT GGGCTCAGTT TATGAGCAAT ATTACACAGG TGGATGTACA 
    AAGACAGAGG AGGAAGAAGC CGCGAGCTCC GAGGAAGACT CAGGCTCTTT TCACGGCTCT 
    GGGGTCTGCA AATGGTTTAA CGTGCGGATG GGCTTCGGAT TTCTGTCCAT GACCCACCGG 
    GAGGGCATCT GTCTGGACTC ACCCGTGGAT GTCTTCGTCC ATCAGAGCAA GCTTCATATG 
    GAGGGTTTTC GCAGTCTGAA GGAAGGAGAA GCAGTGGAAT TCACCTTTAA AAGATCCTCT 
    AAGGGTCTGG AGTCCTTACA AGTGACGGGG CCTGGAGGGG CACCATGTGT GGGCAGCGAG 
    AAGAAACCTA AGGGTACCCA AAAGAGGCGG TCAAAAGGAG ATCGATGCTT CAACTGTGGA 
    GGACCCAATC ATCATGCCAA AGAATGCCAG CTGCCACCTC AGCCGAAAAA GTGCCATTTT 
    TGCCAAAGCA TCTCCCACAT GGTGGCCAAC TGCCCAATCA AAGCACAGCA GTTGTCACCA 
    GGATCTCAGG GCAAATCGAC AACCTCAACA GGAGAAGAGG AGGACATGAG CCACACTCCC 
    TTACTGCCAG AGAGCACTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    lin28a ( XM_009294134.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292409.1 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa24011
    Clone ID Related Accession (Same CDS sequence) XM_009294134.2 , XM_009294134.3
    Accession Version XM_009294134.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein lin-28 homolog A isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTGAAG GTGGATGTAC AAAGACAGAG GAGGAAGAAG CCGCGAGCTC CGAGGAAGAC 
    TCAGGCTCTT TTCACGGCTC TGGGGTCTGC AAATGGTTTA ACGTGCGGAT GGGCTTCGGA 
    TTTCTGTCCA TGACCCACCG GGAGGGCATC TGTCTGGACT CACCCGTGGA TGTCTTCGTC 
    CATCAGAGCA AGCTTCATAT GGAGGGTTTT CGCAGTCTGA AGGAAGGAGA AGCAGTGGAA 
    TTCACCTTTA AAAGATCCTC TAAGGGTCTG GAGTCCTTAC AAGTGACGGG GCCTGGAGGG 
    GCACCATGTG TGGGCAGCGA GAAGAAACCT AAGGGTACCC AAAAGAGGCG GTCAAAAGGA 
    GATCGATGCT TCAACTGTGG AGGACCCAAT CATCATGCCA AAGAATGCCA GCTGCCACCT 
    CAGCCGAAAA AGTGCCATTT TTGCCAAAGC ATCTCCCACA TGGTGGCCAA CTGCCCAATC 
    AAAGCACAGC AGTTGTCACC AGGATCTCAG GGCAAATCGA CAACCTCAAC AGGAGAAGAG 
    GAGGACATGA GCCACACTCC CTTACTGCCA GAGAGCACTG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292409.1
    CDS232..816
    Translation

    Target ORF information:

    RefSeq Version XM_009294134.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009294134.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTGAAG GTGGATGTAC AAAGACAGAG GAGGAAGAAG CCGCGAGCTC CGAGGAAGAC 
    TCAGGCTCTT TTCACGGCTC TGGGGTCTGC AAATGGTTTA ACGTGCGGAT GGGCTTCGGA 
    TTTCTGTCCA TGACCCACCG GGAGGGCATC TGTCTGGACT CACCCGTGGA TGTCTTCGTC 
    CATCAGAGCA AGCTTCATAT GGAGGGTTTT CGCAGTCTGA AGGAAGGAGA AGCAGTGGAA 
    TTCACCTTTA AAAGATCCTC TAAGGGTCTG GAGTCCTTAC AAGTGACGGG GCCTGGAGGG 
    GCACCATGTG TGGGCAGCGA GAAGAAACCT AAGGGTACCC AAAAGAGGCG GTCAAAAGGA 
    GATCGATGCT TCAACTGTGG AGGACCCAAT CATCATGCCA AAGAATGCCA GCTGCCACCT 
    CAGCCGAAAA AGTGCCATTT TTGCCAAAGC ATCTCCCACA TGGTGGCCAA CTGCCCAATC 
    AAAGCACAGC AGTTGTCACC AGGATCTCAG GGCAAATCGA CAACCTCAAC AGGAGAAGAG 
    GAGGACATGA GCCACACTCC CTTACTGCCA GAGAGCACTG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    lin28a ( XM_009294134.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292409.1 Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa24011
    Clone ID Related Accession (Same CDS sequence) XM_009294134.2 , XM_009294134.3
    Accession Version XM_009294134.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein lin-28 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTGAAG GTGGATGTAC AAAGACAGAG GAGGAAGAAG CCGCGAGCTC CGAGGAAGAC 
    TCAGGCTCTT TTCACGGCTC TGGGGTCTGC AAATGGTTTA ACGTGCGGAT GGGCTTCGGA 
    TTTCTGTCCA TGACCCACCG GGAGGGCATC TGTCTGGACT CACCCGTGGA TGTCTTCGTC 
    CATCAGAGCA AGCTTCATAT GGAGGGTTTT CGCAGTCTGA AGGAAGGAGA AGCAGTGGAA 
    TTCACCTTTA AAAGATCCTC TAAGGGTCTG GAGTCCTTAC AAGTGACGGG GCCTGGAGGG 
    GCACCATGTG TGGGCAGCGA GAAGAAACCT AAGGGTACCC AAAAGAGGCG GTCAAAAGGA 
    GATCGATGCT TCAACTGTGG AGGACCCAAT CATCATGCCA AAGAATGCCA GCTGCCACCT 
    CAGCCGAAAA AGTGCCATTT TTGCCAAAGC ATCTCCCACA TGGTGGCCAA CTGCCCAATC 
    AAAGCACAGC AGTTGTCACC AGGATCTCAG GGCAAATCGA CAACCTCAAC AGGAGAAGAG 
    GAGGACATGA GCCACACTCC CTTACTGCCA GAGAGCACTG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292409.1
    CDS239..823
    Translation

    Target ORF information:

    RefSeq Version XM_009294134.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lin-28 homolog A (C. elegans) (lin28a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009294134.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTGAAG GTGGATGTAC AAAGACAGAG GAGGAAGAAG CCGCGAGCTC CGAGGAAGAC 
    TCAGGCTCTT TTCACGGCTC TGGGGTCTGC AAATGGTTTA ACGTGCGGAT GGGCTTCGGA 
    TTTCTGTCCA TGACCCACCG GGAGGGCATC TGTCTGGACT CACCCGTGGA TGTCTTCGTC 
    CATCAGAGCA AGCTTCATAT GGAGGGTTTT CGCAGTCTGA AGGAAGGAGA AGCAGTGGAA 
    TTCACCTTTA AAAGATCCTC TAAGGGTCTG GAGTCCTTAC AAGTGACGGG GCCTGGAGGG 
    GCACCATGTG TGGGCAGCGA GAAGAAACCT AAGGGTACCC AAAAGAGGCG GTCAAAAGGA 
    GATCGATGCT TCAACTGTGG AGGACCCAAT CATCATGCCA AAGAATGCCA GCTGCCACCT 
    CAGCCGAAAA AGTGCCATTT TTGCCAAAGC ATCTCCCACA TGGTGGCCAA CTGCCCAATC 
    AAAGCACAGC AGTTGTCACC AGGATCTCAG GGCAAATCGA CAACCTCAAC AGGAGAAGAG 
    GAGGACATGA GCCACACTCC CTTACTGCCA GAGAGCACTG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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