CCT8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCT8
Entrez Gene ID	394037
Full Name	chaperonin containing TCP1, subunit 8 (theta)
Synonyms	fa22h09,wu:fa22h09,zgc:56059
General protein information	Preferred Names chaperonin containing TCP1, subunit 8 (theta) Names T-complex protein 1 subunit theta bdav bette davis
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201062.1	NP_957356.1	T-complex protein 1 subunit theta
XM_005155366.3	XP_005155423.1	T-complex protein 1 subunit theta isoform X1
XM_005155366.2	XP_005155423.1	T-complex protein 1 subunit theta isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010588	XP_314553.3
Saccharomyces cerevisiae (baker's yeast)	CCT8	NP_012526.1
Arabidopsis thaliana (thale cress)	AT3G03960	NP_566219.1
Gallus gallus (chicken)	CCT8	NP_001004389.1
Drosophila melanogaster (fruit fly)	CG8258	NP_610418.1
Magnaporthe oryzae (rice blast fungus)	MGG_00135	XP_003718996.1
Homo sapiens (human)	CCT8	NP_006576.2
	CCT8	XP_001101969.1
Rattus norvegicus (Norway rat)	Cct8	NP_001099367.2
Caenorhabditis elegans (roundworm)	cct-8	NP_500035.3
Kluyveromyces lactis	KLLA0D09350g	XP_453479.1
Eremothecium gossypii	AGOS_ACR045W	NP_983448.2
Xenopus tropicalis (tropical clawed frog)	cct8	NP_001007875.1
Pan troglodytes (chimpanzee)	CCT8	XP_531416.2
Danio rerio (zebrafish)	cct8	NP_957356.1
Oryza sativa (rice)	Os03g0804800	NP_001051623.1
Canis lupus familiaris (dog)	CCT8	XP_535576.3
Bos taurus (cattle)	CCT8	NP_001028781.1
Mus musculus (house mouse)	Cct8	NP_033970.3
Schizosaccharomyces pombe (fission yeast)	cct8	NP_595406.1
Neurospora crassa	NCU07567	XP_958612.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

The spatial-temporal expression and functional divergence of bach homologs in zebrafish Danio rerio.
Luo Q, Wu C, Sun S, Lu F, Xie L, Zhao H, Zhong X, Zhou Q
Journal of fish biology88(4)1584-97(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Nicotine response genetics in the zebrafish.
Petzold AM, Balciunas D, Sivasubbu S, Clark KJ, Bedell VM, Westcot SE, Myers SR, Moulder GL, Thomas MJ, Ekker SC
Proceedings of the National Academy of Sciences of the United States of America106(44)18662-7(2009 Nov)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCT8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCT8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32727	NM_201062.1 Latest version!	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa16156	XM_005155366.2 Latest version!	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa147595	XM_005155366.3 Latest version!	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCT8 ( NM_201062.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_957356.1 Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), mRNA.

Price & Availability↑

CloneID	ODa32727
Clone ID Related Accession (Same CDS sequence)	NM_201062.1
Accession Version	NM_201062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	T-complex protein 1 subunit theta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050492.1. ##Evidence-Data-START## Transcript exon combination :: BC050492.1, AY391413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCATTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACGGATAACC TGATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCATATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAACT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TAATAATGGC CAAGCCGGCT GGTGGACCCA AAGCTCCACA AGGAAAGAAG 
GACTGGGATG AGGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957356.1
CDS	75..1715
Translation	MALHVPKAPGFAQMLKDGAKHYSGLEEAVFRNIQACKELAQTTRTAYGPNGMNKMVINHLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEELLRMGLSVSEVIEGYEMACKKALEILPDCVCSSAKNLHDLEEATAMIRSAVMSKQYGNEDFLANLIAQACVSIFPESGSFNVDNVRVCKILGCGLNSSTMLHGMVFKKEAEGDVTSVKDAKIAVFSCPFDCTVTETKGTVLIKNADELMNFSKGEEDLMEAQVKAIADSGASVVVTGGKVADMALHYANKYKLMVVRLNSKWDLRRLCKTVGATALPRLTSPTPEEMGRCDSVYLSEVGDTQVVVFKHDKEDGAISTLVIRGSTDNLMDDIERAIDDGVNTFKVLVRDARLCPGAGATEIELAKHITSYGESCPGLEQYAIKKYAEAFEALPRALAENSGVKGNELISKLYATHHEGNKNSGFDIEGEGPAVRDMMEAGILDPYLVKYWGIKLATNAAITVLRVDQIIMAKPAGGPKAPQGKKDWDEDE

Target ORF information:

RefSeq Version	NM_201062.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201062.1

ORF Insert Sequence:

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCATTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACGGATAACC TGATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCATATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAACT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TAATAATGGC CAAGCCGGCT GGTGGACCCA AAGCTCCACA AGGAAAGAAG 
GACTGGGATG AGGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCT8 ( XM_005155366.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155423.1 Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16156
Clone ID Related Accession (Same CDS sequence)	XM_005155366.2
Accession Version	XM_005155366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	T-complex protein 1 subunit theta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005155366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCGTTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACAGATAACC TGATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCATATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAATT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TCATTATGGC AAAGCCAGCA GGGGGACCCA AAGCCCCCAA ACAGCAAGGA 
CACTGGGACA AGGACGGCTG GGATGATGAA CCAGATAAAT TTGATGCACA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155423.1
CDS	105..1781
Translation	MALHVPKAPGFAQMLKDGAKHYSGLEEAVFRNIQACKELAQTTRTAYGPNGMNKMVINHLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEELLRMGLSVSEVIEGYEMACKKALEILPDCVCSSAKNLHDLEEATAMIRSAVMSKQYGNEDFLANLIAQACVSIFPESGSFNVDNVRVCKILGCGLNSSTMLHGMVFKKEAEGDVTSVKDAKIAVFSCPFDCTVTETKGTVLIKNADELMNFSKGEEDLMEAQVKAIADSGASVVVTGGKVADMALHYANKYKLMVVRLNSKWDLRRLCKTVGATALPRLTSPTPEEMGRCDSVYLSEVGDTQVVVFKHDKEDGAISTLVIRGSTDNLMDDIERAIDDGVNTFKVLVRDARLCPGAGATEIELAKHITSYGESCPGLEQYAIKKYAEAFEALPRALAENSGVKGNELISKLYATHHEGNKNSGFDIEGEGPAVRDMMEAGILDPYLVKYWGIKLATNAAITVLRVDQIIMAKPAGGPKAPKQQGHWDKDGWDDEPDKFDAHH

Target ORF information:

RefSeq Version	XM_005155366.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155366.2

ORF Insert Sequence:

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCGTTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACAGATAACC TGATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCATATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAATT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TCATTATGGC AAAGCCAGCA GGGGGACCCA AAGCCCCCAA ACAGCAAGGA 
CACTGGGACA AGGACGGCTG GGATGATGAA CCAGATAAAT TTGATGCACA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCT8 ( XM_005155366.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155423.1 Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147595
Clone ID Related Accession (Same CDS sequence)	XM_005155366.3
Accession Version	XM_005155366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-complex protein 1 subunit theta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCGTTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACAGATAACC TAATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCGTATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAATT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TCATTATGGC AAAGCCAGCA GGGGGACCCA AAGCCCCTAA ACAGCAAGGA 
CACTGGGACA AGGACGGCTG GGATGATGAA CCAGATAAAT TTGATGCACA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155423.1
CDS	105..1781
Translation	MALHVPKAPGFAQMLKDGAKHYSGLEEAVFRNIQACKELAQTTRTAYGPNGMNKMVINHLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEELLRMGLSVSEVIEGYEMACKKALEILPDCVCSSAKNLHDLEEATAMIRSAVMSKQYGNEDFLANLIAQACVSIFPESGSFNVDNVRVCKILGCGLNSSTMLHGMVFKKEAEGDVTSVKDAKIAVFSCPFDCTVTETKGTVLIKNADELMNFSKGEEDLMEAQVKAIADSGASVVVTGGKVADMALHYANKYKLMVVRLNSKWDLRRLCKTVGATALPRLTSPTPEEMGRCDSVYLSEVGDTQVVVFKHDKEDGAISTLVIRGSTDNLMDDIERAIDDGVNTFKVLVRDARLCPGAGATEIELAKHITSYGESCPGLEQYAIKKYAEAFEALPRALAENSGVKGNELISKLYATHHEGNKNSGFDIEGEGPAVRDMMEAGILDPYLVKYWGIKLATNAAITVLRVDQIIMAKPAGGPKAPKQQGHWDKDGWDDEPDKFDAHH

Target ORF information:

RefSeq Version	XM_005155366.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chaperonin containing TCP1, subunit 8 (theta) (cct8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155366.3

ORF Insert Sequence:

ATGGCTCTTC ACGTCCCCAA AGCTCCGGGC TTTGCTCAGA TGTTAAAGGA TGGTGCGAAG 
CACTATTCAG GTCTTGAGGA AGCTGTTTTC AGGAACATAC AGGCATGCAA GGAATTGGCA 
CAAACCACAA GAACTGCTTA TGGACCCAAT GGTATGAACA AAATGGTCAT CAACCACTTG 
GAGAAACTGT TTGTCACTAA TGATGCTGCA ACTATTCTGA GAGAGCTGGA GGTTCAGCAC 
CCAGCAGCCA AAATGATAGT GATGGCGTCC CACATGCAGG AGCAGGAAGT AGGCGATGGC 
ACAAACTTTG TTCTGGTGTT TGCTGGAGCT CTGCTGGAGC TTGCAGAGGA GCTACTGAGA 
ATGGGTCTTT CAGTTTCAGA GGTGATTGAG GGCTATGAGA TGGCTTGTAA GAAAGCTCTG 
GAAATTCTGC CTGATTGCGT CTGCTCTTCT GCAAAGAATC TGCATGATCT GGAGGAGGCG 
ACAGCCATGA TCCGCTCTGC TGTCATGAGC AAACAATACG GCAATGAGGA TTTCCTGGCC 
AACCTCATTG CACAGGCCTG CGTCTCCATC TTCCCTGAGT CTGGTAGTTT TAATGTTGAC 
AATGTCAGAG TATGCAAAAT TTTGGGCTGT GGACTGAACT CTTCTACTAT GCTTCACGGG 
ATGGTGTTTA AGAAAGAGGC AGAAGGTGAT GTCACATCAG TGAAAGATGC AAAGATAGCA 
GTGTTCTCCT GTCCGTTTGA CTGCACGGTT ACTGAAACCA AGGGAACAGT ACTTATCAAG 
AATGCAGATG AGCTCATGAA TTTCAGCAAA GGAGAGGAGG ACCTGATGGA GGCCCAGGTG 
AAGGCTATTG CGGATTCAGG GGCCAGTGTG GTTGTGACTG GGGGCAAAGT CGCTGATATG 
GCTCTTCATT ATGCCAACAA GTACAAGCTC ATGGTGGTCA GGCTTAACTC AAAATGGGAC 
CTCAGAAGAC TATGCAAAAC TGTCGGAGCC ACAGCATTGC CCAGACTGAC TTCCCCTACC 
CCAGAAGAGA TGGGACGCTG TGACAGTGTT TACCTGTCTG AGGTTGGAGA TACACAGGTT 
GTGGTCTTCA AACATGATAA AGAAGACGGT GCCATCTCTA CTTTAGTCAT TCGCGGGTCC 
ACAGATAACC TAATGGATGA CATTGAGCGT GCCATTGATG ATGGAGTGAA CACTTTCAAA 
GTCCTTGTCA GGGATGCACG TCTTTGTCCA GGTGCTGGTG CCACTGAAAT TGAGCTGGCG 
AAGCACATCA CCTCGTATGG AGAATCTTGC CCAGGCCTGG AGCAGTACGC CATCAAGAAG 
TACGCAGAGG CATTTGAAGC ACTGCCCCGT GCGCTGGCAG AAAACTCTGG TGTGAAGGGC 
AACGAGCTGA TCTCCAAATT GTACGCCACA CATCACGAGG GCAACAAGAA CTCAGGCTTT 
GACATTGAGG GAGAGGGTCC TGCAGTGAGA GACATGATGG AGGCAGGCAT CCTCGATCCA 
TACCTAGTCA AGTACTGGGG CATCAAACTG GCCACAAACG CAGCCATCAC TGTCCTCCGT 
GTGGATCAGA TCATTATGGC AAAGCCAGCA GGGGGACCCA AAGCCCCTAA ACAGCAAGGA 
CACTGGGACA AGGACGGCTG GGATGATGAA CCAGATAAAT TTGATGCACA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The spatial-temporal expression and functional divergence of bach homologs in zebrafish Danio rerio.
Journal of fish biology88(4)1584-97(2016 Apr)
Luo Q,Wu C,Sun S,Lu F,Xie L,Zhao H,Zhong X,Zhou Q

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Nicotine response genetics in the zebrafish.
Proceedings of the National Academy of Sciences of the United States of America106(44)18662-7(2009 Nov)
Petzold AM,Balciunas D,Sivasubbu S,Clark KJ,Bedell VM,Westcot SE,Myers SR,Moulder GL,Thomas MJ,Ekker SC

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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CCT8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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