brpf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	brpf1
Entrez Gene ID	393991
Full Name	bromodomain and PHD finger containing, 1
Synonyms	zgc:55530
General protein information	Preferred Names bromodomain and PHD finger containing, 1 Names peregrin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201016.1	NP_957310.1	peregrin

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BRPF1	NP_001026635.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007617	XP_564621.3
Canis lupus familiaris (dog)	BRPF1	XP_850615.1
Mus musculus (house mouse)	Brpf1	NP_001269055.1
Danio rerio (zebrafish)	brpf1	NP_957310.1
Drosophila melanogaster (fruit fly)	Br140	NP_610266.1
Xenopus tropicalis (tropical clawed frog)	brpf1	NP_001106468.1
Pan troglodytes (chimpanzee)	BRPF1	XP_003309650.1
Bos taurus (cattle)	BRPF1	NP_001193018.1
Rattus norvegicus (Norway rat)	Brpf1	NP_001178501.1
Homo sapiens (human)	BRPF1	NP_001003694.1
	BRPF1	XP_001094039.1

Gene Ontology
Bibliography

Related articles in PubMed

Phenoscape: Identifying Candidate Genes for Evolutionary Phenotypes.
Edmunds RC, Su B, Balhoff JP, Eames BF, Dahdul WM, Lapp H, Lundberg JG, Vision TJ, Dunham RA, Mabee PM, Westerfield M
Molecular biology and evolution33(1)13-24(2016 Jan)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
B?hne A, Darras A, D'Cotta H, Baroiller JF, Galiana-Arnoux D, Volff JN
BMC genomics11721(2010 Dec)

The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity.
Laue K, Daujat S, Crump JG, Plaster N, Roehl HH, , Kimmel CB, Schneider R, Hammerschmidt M
Development (Cambridge, England)135(11)1935-46(2008 Jun)

Semaphorin-plexin signaling guides patterning of the developing vasculature.
Torres-V?zquez J, Gitler AD, Fraser SD, Berk JD, Van N Pham, Fishman MC, Childs S, Epstein JA, Weinstein BM
Developmental cell7(1)117-23(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data conclude that Brpf1, coordinated by its particular set of domains, acts by multiple mechanisms to mediate Moz-dependent histone acetylation and to mark Hox genes for maintained expression throughout vertebrate development.
Title: The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity.

The following brpf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brpf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31895	NM_201016.1 Latest version!	Danio rerio bromodomain and PHD finger containing, 1 (brpf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brpf1 ( NM_201016.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957310.1 Danio rerio bromodomain and PHD finger containing, 1 (brpf1), mRNA.

Price & Availability↑

CloneID	ODa31895
Clone ID Related Accession (Same CDS sequence)	NM_201016.1
Accession Version	NM_201016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	peregrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044418.1. On Jul 6, 2005 this sequence version replaced XM_697336.1. ##Evidence-Data-START## Transcript exon combination :: BC044418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCTGG ACTTTGACGT GAAGACTTTC TGCCACAACC TGCGGGCCAC CAAACCGCCG 
TACGAGTGCC CGGTGGAGAG CTGCCGTAAG GTGTACAAGA GCTACAGCGG CATCGAGTAC 
CACCTGTACC ACTACGACCA CGAGAACCCA CCGCCACCAC CGCAGGGGAC GCCACAGAAG 
AAGCGCAAGG GCCGCCCGCC TCGAGGATCC AGCGATGGTC CTGATTCCAG CACAGGCCCC 
GGGGCCACCA GCGGGCAGAA CACGGCTCCG AGCAGCCCTG CAGAGCGCTC CGAACGCTCG 
CACTCACCCG TCAGGGAGAC CATGACCTAC GCTGAGGCCC AGCGCATGGT GGAGATGGAG 
ATCCGCGGGC GCGTGCACCG CGTCAGCATC TTCCAGAATC TGGACGTGGT CTCTGAGGAG 
GACAGCGGGG CTGAGGAGCC TCCAGGGGCC ACGGGGAGTA CAGCCGCAGC CTGTAACGGA 
GGCGGCGAGA GCGGAGGTGG CGACAGAATG GACACAGCAA ACGGGGAGAA AGGAGCGCAG 
AAAACAGGGA AGCACAAGAG TAAGGAGAAG AAGAAGGACA GCGGAGCACA TCATCACATC 
AACACCTCAG GACCCGCCGT CAAACTGCCC GAGGTGGTGT TCCGAGAGCT GGATCAGGAC 
AGACCCGACG CGCCGCCCAG ACCCATGAGC TACTACAGGT ACATCGATAA ATCTGCGGAG 
GAGCTGGACG AGGAGGTGGA GTACGACATC GATGAGGAGG ACTACATCTG GCTGGACATC 
ATGAACGAGA AGCGGCGGTC TGACGGCGTG GCCCCCATCC CGCAGGAGGT GTTCGAGTAT 
CTGATGGACC GCCTGGAGAA AGAGTCCTAC TTCGAGAGCC ACAATAAGGG CGACCCGAGC 
GCTCTGATTG ACGAGGATGC GGTCTGCTGC ATCTGTAACG ACGGCGAGTG TCAGAACAGT 
AACGTGATCC TGTTCTGCGA CATGTGTAAC CTGGCGGTGC ACCAGGAGTG TTACGGCGTG 
CCCTACATCC CCGAGGGCCA GTGGCTGTGC CGCCGCTGCC CGCAGTCGCC ATCCAGAGCT 
GTCGACTGCG CACTGTGCCC TAACAAGGGT GGAGCCTTCA AGCAGACCGA CGACTCGCGA 
TGGGCGCATG TGGTGTGTGC GCTCTGGATA CCGGAGGTGT GTTTTGCGAA TACGGTGTTC 
CTGGAGCCGA TCGACAGCAT TGAGCACATC CCGCCGGCAC GCTGGAAACT AACCTGCTAC 
ATCTGTAAGC AGCGCGGCTC CGGCGCCTGC ATCCAGTGCC ACAAGGCGAA CTGCTACACT 
GCCTTCCACG TCACCTGCGC TCAGCAGGCC GGCCTCTACA TGAAGATGGA GCCGGTGCGG 
GAGACCGGAG CCAACGGCAC CTCCTTCAGC GTACGCAAGA CCGCCTACTG CGACATACAC 
ACACCGCCCG GCTCCGCACG CCCGCTCGGG GGAGTGGGCG GGGTCAGCGC TGACTCCTCC 
CGCAGCGAAG CAGAGCCAGA GGAGGAGGAG GAGCCGCCGG CTGCTGAGGA TGACGGGAAG 
GGCTGGAGCT CGGAGCGGGC GAAGAGAGCG AAGGCGAAGT CACGGCTGAA GATGAAGAGA 
GCCAGGAAGA TCCTGGCGGA GCGCAGAGCG GCCGCACCCG TGCTGTCTGT GCCCTGCATA 
CCACCACACA AGCTGAGTAA GATCACCAGT AACCTGACGG TGCAGAGGAA GAGTCAGTTC 
ATGCAGCGAC TGCACAGCTA CTGGACCCTG AAGAGGCAGA GCCGTAATGG CGTCCCGCTG 
CTGAGGCGGC TGCAGACGCA CCTGCAGTCA CAGCGCAACA CAGAGCCGCT GCCCGTCACG 
AGGGACGGTG AGGAGAAACA CTCGGTGCTG AAGGAGCAGC TGAAGGCGTG GCAGCGTCTC 
CGTCATGATC TGGAGAGAGC CAGACTGCTG GTGGAGCTGA TCCGCAAGAG GGAGAAGCTG 
AAGAGAGAGA CGATTAAGGT GCAGGAGATG GCGATGGAGA TGCAGCTCAC TCCACTGCTG 
GTGTTGCTGC GCAGAACACT GGAGCAGCTG CAGGAGAGAG ACACCAACAA CTTCTTCACT 
GAGCCCGTAC CGCTGAGCGA GGTCCCGGAC TACCTAGACC ACATCGAGCG GCCGATGGAC 
TTCCATAAGA TGTGGAAGTG TGTGGAGTCG CACCGCTACC TGAGCTTCGA GGCGTTCGAG 
GGCGATTTCC TGCAGATCGT CAACAACTGC CTGAAGTACA ACGCTAAAGA CACGGTGTTC 
TACCGCGCGG CGCTGCGGCT GCGGGAGGCG GGAGGAGTGG TGCTGCGGCA GGCGCGCAGG 
CAGGCCGAGC GCATCGGCTT CGACTACGAG ACGGGTCTGC ATCTGCACCG AGAGCCCAGC 
CCCGAAAACA CACACACACA CCAGCGGGAC AGAGAGCGGG AGCGCGTCTA CATAGAGGGC 
GAGCTGCTGT CGGAGAGCTG GCGGCGCCTC CCTGTGGACG AGCAGCTGCA GATCCTGCAG 
AGCCGCTATG AGGAGGCGGC GTCCGGGAAA CACAGCATCG GCCAGCAGCG CAGAGCCAAA 
GCGCTGAAGA AGGAGATGAC GGCGCTGCGG AGGAAGCTGG CGCACCCACG CGACACACTC 
CTGCTGGACA CACACTCTCA CACACACATA CTGAGTAAAG ACGGTGTGTG TGTGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957310.1
CDS	160..2859
Translation	MGLDFDVKTFCHNLRATKPPYECPVESCRKVYKSYSGIEYHLYHYDHENPPPPPQGTPQKKRKGRPPRGSSDGPDSSTGPGATSGQNTAPSSPAERSERSHSPVRETMTYAEAQRMVEMEIRGRVHRVSIFQNLDVVSEEDSGAEEPPGATGSTAAACNGGGESGGGDRMDTANGEKGAQKTGKHKSKEKKKDSGAHHHINTSGPAVKLPEVVFRELDQDRPDAPPRPMSYYRYIDKSAEELDEEVEYDIDEEDYIWLDIMNEKRRSDGVAPIPQEVFEYLMDRLEKESYFESHNKGDPSALIDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCPQSPSRAVDCALCPNKGGAFKQTDDSRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARPLGGVGGVSADSSRSEAEPEEEEEPPAAEDDGKGWSSERAKRAKAKSRLKMKRARKILAERRAAAPVLSVPCIPPHKLSKITSNLTVQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNTEPLPVTRDGEEKHSVLKEQLKAWQRLRHDLERARLLVELIRKREKLKRETIKVQEMAMEMQLTPLLVLLRRTLEQLQERDTNNFFTEPVPLSEVPDYLDHIERPMDFHKMWKCVESHRYLSFEAFEGDFLQIVNNCLKYNAKDTVFYRAALRLREAGGVVLRQARRQAERIGFDYETGLHLHREPSPENTHTHQRDRERERVYIEGELLSESWRRLPVDEQLQILQSRYEEAASGKHSIGQQRRAKALKKEMTALRRKLAHPRDTLLLDTHSHTHILSKDGVCVV

Target ORF information:

RefSeq Version	NM_201016.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bromodomain and PHD finger containing, 1 (brpf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201016.1

ORF Insert Sequence:

ATGGGGCTGG ACTTTGACGT GAAGACTTTC TGCCACAACC TGCGGGCCAC CAAACCGCCG 
TACGAGTGCC CGGTGGAGAG CTGCCGTAAG GTGTACAAGA GCTACAGCGG CATCGAGTAC 
CACCTGTACC ACTACGACCA CGAGAACCCA CCGCCACCAC CGCAGGGGAC GCCACAGAAG 
AAGCGCAAGG GCCGCCCGCC TCGAGGATCC AGCGATGGTC CTGATTCCAG CACAGGCCCC 
GGGGCCACCA GCGGGCAGAA CACGGCTCCG AGCAGCCCTG CAGAGCGCTC CGAACGCTCG 
CACTCACCCG TCAGGGAGAC CATGACCTAC GCTGAGGCCC AGCGCATGGT GGAGATGGAG 
ATCCGCGGGC GCGTGCACCG CGTCAGCATC TTCCAGAATC TGGACGTGGT CTCTGAGGAG 
GACAGCGGGG CTGAGGAGCC TCCAGGGGCC ACGGGGAGTA CAGCCGCAGC CTGTAACGGA 
GGCGGCGAGA GCGGAGGTGG CGACAGAATG GACACAGCAA ACGGGGAGAA AGGAGCGCAG 
AAAACAGGGA AGCACAAGAG TAAGGAGAAG AAGAAGGACA GCGGAGCACA TCATCACATC 
AACACCTCAG GACCCGCCGT CAAACTGCCC GAGGTGGTGT TCCGAGAGCT GGATCAGGAC 
AGACCCGACG CGCCGCCCAG ACCCATGAGC TACTACAGGT ACATCGATAA ATCTGCGGAG 
GAGCTGGACG AGGAGGTGGA GTACGACATC GATGAGGAGG ACTACATCTG GCTGGACATC 
ATGAACGAGA AGCGGCGGTC TGACGGCGTG GCCCCCATCC CGCAGGAGGT GTTCGAGTAT 
CTGATGGACC GCCTGGAGAA AGAGTCCTAC TTCGAGAGCC ACAATAAGGG CGACCCGAGC 
GCTCTGATTG ACGAGGATGC GGTCTGCTGC ATCTGTAACG ACGGCGAGTG TCAGAACAGT 
AACGTGATCC TGTTCTGCGA CATGTGTAAC CTGGCGGTGC ACCAGGAGTG TTACGGCGTG 
CCCTACATCC CCGAGGGCCA GTGGCTGTGC CGCCGCTGCC CGCAGTCGCC ATCCAGAGCT 
GTCGACTGCG CACTGTGCCC TAACAAGGGT GGAGCCTTCA AGCAGACCGA CGACTCGCGA 
TGGGCGCATG TGGTGTGTGC GCTCTGGATA CCGGAGGTGT GTTTTGCGAA TACGGTGTTC 
CTGGAGCCGA TCGACAGCAT TGAGCACATC CCGCCGGCAC GCTGGAAACT AACCTGCTAC 
ATCTGTAAGC AGCGCGGCTC CGGCGCCTGC ATCCAGTGCC ACAAGGCGAA CTGCTACACT 
GCCTTCCACG TCACCTGCGC TCAGCAGGCC GGCCTCTACA TGAAGATGGA GCCGGTGCGG 
GAGACCGGAG CCAACGGCAC CTCCTTCAGC GTACGCAAGA CCGCCTACTG CGACATACAC 
ACACCGCCCG GCTCCGCACG CCCGCTCGGG GGAGTGGGCG GGGTCAGCGC TGACTCCTCC 
CGCAGCGAAG CAGAGCCAGA GGAGGAGGAG GAGCCGCCGG CTGCTGAGGA TGACGGGAAG 
GGCTGGAGCT CGGAGCGGGC GAAGAGAGCG AAGGCGAAGT CACGGCTGAA GATGAAGAGA 
GCCAGGAAGA TCCTGGCGGA GCGCAGAGCG GCCGCACCCG TGCTGTCTGT GCCCTGCATA 
CCACCACACA AGCTGAGTAA GATCACCAGT AACCTGACGG TGCAGAGGAA GAGTCAGTTC 
ATGCAGCGAC TGCACAGCTA CTGGACCCTG AAGAGGCAGA GCCGTAATGG CGTCCCGCTG 
CTGAGGCGGC TGCAGACGCA CCTGCAGTCA CAGCGCAACA CAGAGCCGCT GCCCGTCACG 
AGGGACGGTG AGGAGAAACA CTCGGTGCTG AAGGAGCAGC TGAAGGCGTG GCAGCGTCTC 
CGTCATGATC TGGAGAGAGC CAGACTGCTG GTGGAGCTGA TCCGCAAGAG GGAGAAGCTG 
AAGAGAGAGA CGATTAAGGT GCAGGAGATG GCGATGGAGA TGCAGCTCAC TCCACTGCTG 
GTGTTGCTGC GCAGAACACT GGAGCAGCTG CAGGAGAGAG ACACCAACAA CTTCTTCACT 
GAGCCCGTAC CGCTGAGCGA GGTCCCGGAC TACCTAGACC ACATCGAGCG GCCGATGGAC 
TTCCATAAGA TGTGGAAGTG TGTGGAGTCG CACCGCTACC TGAGCTTCGA GGCGTTCGAG 
GGCGATTTCC TGCAGATCGT CAACAACTGC CTGAAGTACA ACGCTAAAGA CACGGTGTTC 
TACCGCGCGG CGCTGCGGCT GCGGGAGGCG GGAGGAGTGG TGCTGCGGCA GGCGCGCAGG 
CAGGCCGAGC GCATCGGCTT CGACTACGAG ACGGGTCTGC ATCTGCACCG AGAGCCCAGC 
CCCGAAAACA CACACACACA CCAGCGGGAC AGAGAGCGGG AGCGCGTCTA CATAGAGGGC 
GAGCTGCTGT CGGAGAGCTG GCGGCGCCTC CCTGTGGACG AGCAGCTGCA GATCCTGCAG 
AGCCGCTATG AGGAGGCGGC GTCCGGGAAA CACAGCATCG GCCAGCAGCG CAGAGCCAAA 
GCGCTGAAGA AGGAGATGAC GGCGCTGCGG AGGAAGCTGG CGCACCCACG CGACACACTC 
CTGCTGGACA CACACTCTCA CACACACATA CTGAGTAAAG ACGGTGTGTG TGTGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phenoscape: Identifying Candidate Genes for Evolutionary Phenotypes.
Molecular biology and evolution33(1)13-24(2016 Jan)
Edmunds RC,Su B,Balhoff JP,Eames BF,Dahdul WM,Lapp H,Lundberg JG,Vision TJ,Dunham RA,Mabee PM,Westerfield M

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
BMC genomics11721(2010 Dec)
B?hne A,Darras A,D'Cotta H,Baroiller JF,Galiana-Arnoux D,Volff JN

The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity.
Development (Cambridge, England)135(11)1935-46(2008 Jun)
Laue K,Daujat S,Crump JG,Plaster N,Roehl HH,,Kimmel CB,Schneider R,Hammerschmidt M

Semaphorin-plexin signaling guides patterning of the developing vasculature.
Developmental cell7(1)117-23(2004 Jul)
Torres-V?zquez J,Gitler AD,Fraser SD,Berk JD,Van N Pham,Fishman MC,Childs S,Epstein JA,Weinstein BM

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brpf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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