atp2a2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp2a2a
Entrez Gene ID	393940
Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2a
Synonyms	atp2a2,zgc:55380
General protein information	Preferred Names ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2a Names sarcoplasmic/endoplasmic reticulum calcium ATPase 2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a serca2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478220.1	XP_021333895.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X2
XM_005166799.3	XP_005166856.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
XM_005166798.3	XP_005166855.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
XM_005166799.4	XP_005166856.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
NM_200965.1	NP_957259.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
XM_005166798.4	XP_005166855.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP2A2	NP_001178359.1
Rattus norvegicus (Norway rat)	Atp2a2	NP_001104293.1
Mus musculus (house mouse)	Atp2a2	NP_001103610.1
Pan troglodytes (chimpanzee)	ATP2A2	XP_001141455.1
Gallus gallus (chicken)	ATP2A2	NP_001258903.1
Xenopus tropicalis (tropical clawed frog)	atp2a2	XP_004910568.1
Homo sapiens (human)	ATP2A2	NP_733765.1
Canis lupus familiaris (dog)	ATP2A2	NP_001003214.1
Danio rerio (zebrafish)	atp2a2a	NP_957259.1

Gene Ontology
Bibliography

Related articles in PubMed

Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy.
Nagata Y, Yamagishi M, Konno T, Nakanishi C, Asano Y, Ito S, Nakajima Y, Seguchi O, Fujino N, Kawashiri MA, Takashima S, Kitakaze M, Hayashi K
Scientific reports7(1)17417(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following atp2a2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp2a2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00853	NM_200965.1 Latest version!	Danio rerio ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2a (atp2a2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13946	XM_021478220.1 Latest version!	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa13945	XM_005166798.4 Latest version!	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13945	XM_005166799.4 Latest version!	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa13945	XM_005166799.3	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13945	XM_005166798.3	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp2a2a ( NM_200965.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957259.1 Danio rerio ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2a (atp2a2a), mRNA.

Price & Availability↑

CloneID	ODa00853
Clone ID Related Accession (Same CDS sequence)	NM_200965.1
Accession Version	NM_200965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045327.1. On Jul 6, 2005 this sequence version replaced XM_697329.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAACTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGTGAGT CTGTATCTGT CATCAAGCAC ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTGGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATACGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAGTA TGATGGTTTG GTGGAGCTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CTTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT ACGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATATTC 
TCTGATGATG ATGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCTCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTATTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCAACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATCGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATCCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGCTC CAGAGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957259.1
CDS	216..3206
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDDDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPAPED

Target ORF information:

RefSeq Version	NM_200965.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2a (atp2a2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200965.1

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAACTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGTGAGT CTGTATCTGT CATCAAGCAC ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTGGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATACGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAGTA TGATGGTTTG GTGGAGCTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CTTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT ACGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATATTC 
TCTGATGATG ATGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCTCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTATTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCAACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATCGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATCCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGCTC CAGAGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2a2a ( XM_021478220.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333895.1 Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13946
Clone ID Related Accession (Same CDS sequence)	XM_021478220.1
Accession Version	XM_021478220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGCTC CAGAGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333895.1
CDS	242..3232
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDEDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPAPED

Target ORF information:

RefSeq Version	XM_021478220.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478220.1

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGCTC CAGAGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2a2a ( XM_005166799.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166856.1 Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13945
Clone ID Related Accession (Same CDS sequence)	XM_005166799.3 , XM_005166798.3 , XM_005166799.4 , XM_005166798.4
Accession Version	XM_005166799.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166856.1
CDS	231..3359
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDEDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPGKDLKEEKEDRRGWSFSACTDGVSWPFIALTLPLVVWLYSTNITQLFRHS

Target ORF information:

RefSeq Version	XM_005166799.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166799.3

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2a2a ( XM_005166798.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166855.1 Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13945
Clone ID Related Accession (Same CDS sequence)	XM_005166799.3 , XM_005166798.3 , XM_005166799.4 , XM_005166798.4
Accession Version	XM_005166798.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166855.1
CDS	235..3363
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDEDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPGKDLKEEKEDRRGWSFSACTDGVSWPFIALTLPLVVWLYSTNITQLFRHS

Target ORF information:

RefSeq Version	XM_005166798.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166798.3

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2a2a ( XM_005166799.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166856.1 Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13945
Clone ID Related Accession (Same CDS sequence)	XM_005166799.3 , XM_005166798.3 , XM_005166799.4 , XM_005166798.4
Accession Version	XM_005166799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166799.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166856.1
CDS	238..3366
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDEDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPGKDLKEEKEDRRGWSFSACTDGVSWPFIALTLPLVVWLYSTNITQLFRHS

Target ORF information:

RefSeq Version	XM_005166799.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166799.4

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2a2a ( XM_005166798.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166855.1 Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13945
Clone ID Related Accession (Same CDS sequence)	XM_005166799.3 , XM_005166798.3 , XM_005166799.4 , XM_005166798.4
Accession Version	XM_005166798.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166798.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166855.1
CDS	242..3370
Translation	MENAHTKSVEEVYSNFSVNESTGLTLDQVKRNRDKWGPNELPAEEGKSIWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEVAVGDKVPADIRISAIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAIGVVVATGVNTEIGKIRDEMASTEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKAEGENCSLTEFTISGSTYAPEGDVCLDNRIVKCSQYDGLVELATICALCNDSSLDFNESKGVYEKVGEATETALTCLVEKMNVFDTDVRNLSKIERANACNAVIKQLMKKEFTLEFSRDRKSMSVYCSPNKAKSSSSKMFVKGAPEGVIDRCAYVRVGGSKVPLTQGIKDKIMSVIREYGTGRDTLRCLALATRDNPLKKEEMVLSDTARFADYESDLTFVGCVGMLDPPRTEVAASIKLCRHAGIRVIMITGDNKGTAVAICRRIGIFSDEDDVHRMAFTGREFDDLSPHAQREAVTVARCFARVEPSHKSKIVEFLQGFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRSAKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIVADDGPMITLYQLSHFLQCSPDNPDFQDLECHVFESPYPMTMALSVLVTIEMCNALNSLSENQSLVRMPPWENIWLLGAICLSMSLHFLILYVEPLPVIFQITPLNVTQWMMVLKISLPVILLDELLKFVARTYLEPGKDLKEEKEDRRGWSFSACTDGVSWPFIALTLPLVVWLYSTNITQLFRHS

Target ORF information:

RefSeq Version	XM_005166798.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a (atp2a2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166798.4

ORF Insert Sequence:

ATGGAGAACG CTCACACCAA GAGTGTGGAA GAAGTTTATA GTAATTTCAG CGTCAACGAG 
AGCACGGGAC TGACTTTAGA CCAGGTGAAA AGGAATAGAG ACAAATGGGG TCCTAACGAG 
TTGCCTGCAG AGGAAGGAAA GTCAATATGG GAGCTTGTCA TTGAGCAGTT TGAAGATTTA 
CTTGTGCGGA TTCTTCTACT GGCAGCTTGC ATATCTTTTG TATTAGCCTG GTTCGAGGAG 
GGGGAGGAAA CTATCACTGC CTTTGTGGAG CCTTTTGTAA TCTTACTGAT TCTTATAGCT 
AATGCCATCG TCGGCGTTTG GCAGGAACGT AATGCAGAAA ATGCCATTGA AGCCCTCAAA 
GAGTACGAGC CAGAGATGGG CAAGGTGTAC CGTCAGGACA GGAAGTCCGT GCAAAGGATC 
AAAGCCAAAG ACATCGTGCC TGGAGACATA GTGGAGGTCG CTGTGGGTGA TAAGGTGCCT 
GCCGATATCC GTATCAGTGC CATCAAGTCC ACCACACTCC GAGTGGACCA GTCTATTCTG 
ACAGGCGAGT CTGTATCTGT CATCAAGCAT ACTGACCCCG TGCCGGATCC CCGTGCCGTC 
AATCAGGACA AGAAGAACAT GTTGTTCTCA GGAACCAACA TTGCTGCAGG TAAAGCCATC 
GGCGTAGTGG TGGCCACAGG TGTGAACACA GAGATCGGTA AGATCCGTGA TGAAATGGCC 
TCCACCGAGC AGGAGAGGAC GCCGCTGCAG CAGAAGCTGG ACGAGTTTGG CGAGCAGCTC 
TCTAAAGTCA TCTCCCTGAT CTGCATCGCC GTCTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTCC ACGGTGGCTC CTGGATCCGT GGCGCCGTTT ACTACTTCAA GATCGCCGTG 
GCTCTGGCTG TAGCTGCCAT TCCTGAAGGT CTGCCCGCTG TCATCACCAC CTGCCTGGCT 
CTCGGCACTC GCCGTATGGC CAAGAAGAAC GCCATCGTCC GCTCTCTGCC CTCTGTGGAG 
ACTCTGGGCT GCACCTCAGT CATCTGCTCC GACAAGACTG GCACCCTCAC CACCAACCAG 
ATGTCCGTCT GCAGGATGTT CATCATAGAC AAGGCTGAAG GAGAAAACTG CTCACTGACT 
GAATTCACCA TCTCTGGCTC CACATATGCT CCTGAAGGAG ACGTATGCCT TGACAACAGA 
ATCGTGAAGT GCTCCCAATA TGATGGTTTG GTGGAGTTGG CGACCATCTG TGCCTTGTGC 
AATGACTCCT CCCTGGATTT TAATGAGTCC AAAGGTGTTT ATGAGAAGGT TGGAGAGGCG 
ACAGAAACGG CTCTAACCTG CCTGGTGGAA AAGATGAACG TCTTTGACAC TGACGTCAGG 
AACCTGTCAA AGATCGAGCG AGCCAATGCC TGCAATGCTG TGATCAAGCA GCTGATGAAG 
AAGGAGTTCA CCCTCGAGTT CTCCAGAGAC AGAAAGTCCA TGTCTGTGTA CTGCTCCCCA 
AACAAAGCCA AATCCTCCTC TTCTAAGATG TTTGTCAAGG GAGCTCCAGA AGGGGTGATC 
GATAGGTGTG CATATGTCCG TGTTGGAGGA TCCAAGGTTC CTCTGACTCA GGGCATCAAG 
GACAAGATCA TGTCTGTGAT CCGCGAGTAC GGGACTGGCC GGGATACCCT GCGCTGCCTG 
GCACTGGCCA CCCGTGACAA TCCGCTCAAG AAAGAGGAGA TGGTCCTGTC AGATACCGCC 
CGGTTTGCTG ATTATGAGTC TGATCTAACC TTCGTGGGCT GTGTGGGCAT GCTGGACCCT 
CCTCGAACAG AAGTAGCGGC CTCCATCAAG CTCTGCCGCC ATGCTGGGAT CAGGGTCATC 
ATGATCACCG GTGATAACAA AGGCACGGCC GTGGCCATCT GCAGACGCAT CGGCATCTTC 
TCTGACGAGG ACGACGTCCA CCGCATGGCT TTCACGGGAC GCGAGTTTGA CGACCTGTCG 
CCCCATGCCC AGCGTGAGGC GGTCACTGTG GCCCGCTGCT TCGCACGAGT CGAGCCTTCA 
CACAAGTCCA AGATTGTGGA GTTCCTGCAG GGCTTTGATG AGATCACTGC CATGACAGGA 
GATGGTGTGA ATGATGCCCC TGCCCTGAAA AAGGCAGAGA TTGGCATCGC CATGGGCTCT 
GGCACTGCTG TGGCCAAGAC AGCCTCGGAG ATGGTCCTCG CTGATGACAA CTTTTCCACC 
ATTGTGGCCG CCGTTGAAGA AGGCAGAGCA ATCTACAACA ACATGAAGCA GTTCATCCGC 
TACCTGATCT CCTCCAACGT GGGAGAGGTC GTCTGTATCT TCCTGACCGC TGCATTGGGT 
TTCCCTGAGG CGCTGATCCC CGTCCAGCTG CTGTGGGTGA ACCTGGTGAC TGACGGCTTG 
CCTGCCACCG CTCTAGGCTT CAACCCCCCT GATCTGGACA TCATGAACAA GCCCCCCCGA 
AGCGCCAAAG AGCCCCTCAT CTCCGGATGG CTCTTTTTCA GATACTTGGC CATCGGCTGT 
TATGTGGGTG CTGCTACTGT AGGAGCTGCA GCCTGGTGGT TCATTGTTGC TGATGATGGT 
CCAATGATCA CACTTTACCA GCTGAGTCAT TTCCTGCAGT GTTCTCCCGA TAATCCTGAC 
TTCCAGGACT TGGAGTGTCA CGTCTTCGAG TCTCCTTACC CCATGACCAT GGCCCTGTCT 
GTGCTCGTCA CCATTGAGAT GTGCAACGCC CTCAACAGTC TATCCGAGAA CCAGTCTCTG 
GTGCGCATGC CACCCTGGGA GAATATCTGG CTGCTGGGAG CCATCTGTCT CTCCATGTCT 
CTGCACTTCC TCATCCTCTA TGTGGAGCCA CTGCCGGTCA TCTTCCAGAT CACTCCTCTG 
AATGTGACGC AGTGGATGAT GGTGCTGAAG ATCTCGCTGC CTGTCATTCT CTTGGACGAG 
CTGCTCAAAT TTGTGGCGAG GACCTACCTG GAGCCTGGTA AAGACCTGAA GGAGGAAAAG 
GAGGACAGGA GAGGCTGGTC CTTCTCTGCA TGCACTGACG GCGTCTCCTG GCCCTTCATA 
GCTCTCACAC TCCCTCTGGT GGTGTGGCTG TACAGCACTA ACATAACCCA GCTCTTCCGG 
CACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy.
Scientific reports7(1)17417(2017 Dec)
Nagata Y,Yamagishi M,Konno T,Nakanishi C,Asano Y,Ito S,Nakajima Y,Seguchi O,Fujino N,Kawashiri MA,Takashima S,Kitakaze M,Hayashi K

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atp2a2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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