MLH1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MLH1
Entrez Gene ID	393632
Full Name	mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Synonyms	zgc:66301
General protein information	Preferred Names mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) Names DNA mismatch repair protein Mlh1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307549.2	XP_009305824.1	DNA mismatch repair protein Mlh1 isoform X1
XM_009307549.3	XP_009305824.1	DNA mismatch repair protein Mlh1 isoform X1
NM_200659.1	NP_956953.1	DNA mismatch repair protein Mlh1
XM_009307550.2	XP_009305825.1	DNA mismatch repair protein Mlh1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mlh1	NP_112315.1
Drosophila melanogaster (fruit fly)	Mlh1	NP_477022.1
Magnaporthe oryzae (rice blast fungus)	MGG_07121	XP_003715311.1
Oryza sativa (rice)	Os01g0958900	NP_001045457.1
Xenopus tropicalis (tropical clawed frog)	mlh1	XP_002933455.2
Pan troglodytes (chimpanzee)	MLH1	XP_001170433.1
Canis lupus familiaris (dog)	MLH1	XP_534219.2
Danio rerio (zebrafish)	mlh1	NP_956953.1
Schizosaccharomyces pombe (fission yeast)	mlh1	NP_596199.1
Neurospora crassa	NCU08309	XP_962522.1
Gallus gallus (chicken)	MLH1	XP_418828.1
Saccharomyces cerevisiae (baker's yeast)	MLH1	NP_013890.1
Kluyveromyces lactis	KLLA0D09955g	XP_453504.1
Bos taurus (cattle)	MLH1	NP_001069462.2
Mus musculus (house mouse)	Mlh1	NP_081086.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012192	XP_320342.4
Homo sapiens (human)	MLH1	NP_000240.1
Eremothecium gossypii	AGOS_AFL199C	NP_985351.1
Arabidopsis thaliana (thale cress)	MLH1	NP_567345.2

Gene Ontology
Bibliography

Related articles in PubMed

Xia H, Zhong C, Wu X, Chen J, Tao B, Xia X, Shi M, Zhu Z, Trudeau VL, Hu W
Genetics(2017 Dec)

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Lei L, Yan SY, Yang R, Chen JY, Li Y, Bu Y, Chang N, Zhou Q, Zhu X, Li CY, Xiong JW
Nucleic acids research45(6)3422-3436(2017 Apr)

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Zhang Y, O'Leary MN, Peri S, Wang M, Zha J, Melov S, Kappes DJ, Feng Q, Rhodes J, Amieux PS, Morris DR, Kennedy BK, Wiest DL
Cell reports18(2)545-556(2017 01)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification and characterization of novel knockout mutants of the three major MMR genes, mlh1, msh2, and msh6, in zebrafish that develop tumors at low frequencies.
Title: Zebrafish with mutations in mismatch repair genes develop neurofibromas and other tumors.

In zebrafish mlh1 mutant (knock-out) males, a delay of both meiotic divisions occurs rather than complete arrest during meiosis I. Eggs fertilized with mutant sperm develop as malformed embryos and are aneuploid.
Title: Completion of meiosis in male zebrafish (Danio rerio) despite lack of DNA mismatch repair gene mlh1.

Male mlh1 mutants are sterile and display an arrest in spermatogenesis at metaphase I, resulting in increased testis weight due to accumulation of prophase I spermatocytes.
Title: Mlh1 deficiency in zebrafish results in male sterility and aneuploid as well as triploid progeny in females.

The following MLH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40481	XM_009307549.3 Latest version!	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40482	XM_009307550.2 Latest version!	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa35042	NM_200659.1 Latest version!	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa40481	XM_009307549.2	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MLH1 ( XM_009307549.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305824.1 Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40481
Clone ID Related Accession (Same CDS sequence)	XM_009307549.2 , XM_009307549.3
Accession Version	XM_009307549.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA mismatch repair protein Mlh1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATAG TCTGTGAGCG TTTTACGACC AGCAAGCTGC AGTCTTTTGA AGATTTGTCA 
TCCATCGCAA CATACGGATT CAGAGGAGAA GCCCTCGCCA GTATAAGTCA TGTAGCTCAC 
GTCACCATCA CCACAAAAAC AGCAGATGCT AAATGTGCTT ACAGGGCCAA TTACTGTGAT 
GGGAAACTAA AATCTCCACC CAAACCCTGT GCTGGAAACC AGGGGACACT GATTTCTGTA 
GAGGATCTGT TTTATAATGT GTCTACGCGA CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA 
TATTCCAGAA TCGTCGAGGT TGTGAGCAGA TACGCCATTC ACAACTCTGG GAAAAGTTTT 
TCTGTCAAGA AGCAAGGTGA GATGGTTGCA GATGTGAAGA CTCTCCCAAA CGCCTCTGTG 
CTGGACAACA TCCGTGTGGT GTTTGGAGTC GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA 
TGCGAGGATC AGAAATTTGC TTTTAAAGTG AAGGGCTACA TCTCCAATGC TAACTACTCT 
GTCAAGAAAT GCATCCTTAT CCTTTTTATC AATCATCGAT TGGTGGAGTC TAGTGCCTTG 
AAGAAAGCAA TTGAGACCGT CTACACCGCT TATCTTCCCA AAAACACTCA TCCTTTTCTT 
TATCTCAGTT TAGAGATTGC TCCTCAAAAC ATTGATGTAA ACGTTCACCC GACAAAACAT 
GAGGTGCACT TCCTGCACGA GGACTCCATC ATCGAGAGCA TTCAGAAGCA CATCGAGAGT 
AAACTCCTCG GCTCCAATTC CTCTCGCACG TACTTCACAC AGACTCTACT TCCGGGACTT 
TCAGCATCTG CAAGCGTGGC AAAAGCTTCT AGTTCCTCAG CAGATTCCCA GGAGCGAGTT 
TACGCCCATC AGATGGTCCG CACTGACAGT AAAGCCCAGA AGCTTGATGC ATTTCTCCAG 
CCATCAGCCT CATCTTCATC AACTGCTGCT CAAAGAAAGA CAGAAAAAAC CTCCAGCACG 
TCAACTGCTG TACAAGACTT GGTGGAGCTG GACGACGCAG AGCTGCTGAC AGCTGCTGAT 
GTGGAGCCGT GTGGCGGAAA GGATCCTCAG ACTGATGCTC AACCTCCTGG TGATGAAGCA 
CCACCAAGGA AAAGGCCTCA TGTTGAGGAG GTGAAGGAGG ATTTGACAGC TGCTTCTCTT 
CCCAGGAGAC GAATCGTTAA ACTGACCAGC ATAAAGGAGC TTCGGGACCA GATTGAGCTG 
CAAACTCACA AAGGTTTACA GGAGCTGCTG CAGAATCACT CATTTGTAGG TTCAGTCAGT 
CCTCAGTGGA CTTTGGTGCA GCACCAGACC AAACTCTACC TGCTCAATAC AACCAAACTC 
AGTCAGGAGC TGTTTTATCA AATTTTAGTC TACGACTTTG GTAACTTCGG CGTTCTTCGA 
TTGTCGAATC CAGCGCCGCT CTATGATCTG GCCATGTTGG CTCTGGATTC AGAGGAAAGT 
GGATGGACAG AGGAGGATGG TCCGAAAGAA GGACTGGCCC AGTATATTGT GGATTTCCTT 
AAGCAGAAAG CAGAAATGCT GGAAGAATAC TTTTCCTTGG AAATAGATGC AGAGGGAAAC 
CTGACAGGTC TGCCAATGCT GTTGGATAAT TACACTCCTG CCATGGAGGG ACTGCCAATG 
TTCATTTTGC GTTTAGCCAC TGAGGTGAAC TGGGATAAAG AAAAGGAGTG TTTCCGTGAA 
TTCAGTGTTG AATGCAGTCA TTTCTACTCC ATAAGGAAAA GCTACACACT GGAGGCAGAC 
GCAGATGAGC CACAGGATGC TGAGATGAGC TGGCAGTGGA AGGTGGAGCA TGTGCTTTTC 
AAAGCTTTGC GCTCTCTCTT CAGTCCTGCC AAACACTTGA GTGAAGACGG CAGTGTCCTG 
CAGATCGCCA GCCTGCCTGA CCTCTACAAA GTGTTTGAAA GATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305824.1
CDS	101..2068
Translation	MEIVCERFTTSKLQSFEDLSSIATYGFRGEALASISHVAHVTITTKTADAKCAYRANYCDGKLKSPPKPCAGNQGTLISVEDLFYNVSTRRKALKSPSEEYSRIVEVVSRYAIHNSGKSFSVKKQGEMVADVKTLPNASVLDNIRVVFGVAVSRELIEVECEDQKFAFKVKGYISNANYSVKKCILILFINHRLVESSALKKAIETVYTAYLPKNTHPFLYLSLEIAPQNIDVNVHPTKHEVHFLHEDSIIESIQKHIESKLLGSNSSRTYFTQTLLPGLSASASVAKASSSSADSQERVYAHQMVRTDSKAQKLDAFLQPSASSSSTAAQRKTEKTSSTSTAVQDLVELDDAELLTAADVEPCGGKDPQTDAQPPGDEAPPRKRPHVEEVKEDLTAASLPRRRIVKLTSIKELRDQIELQTHKGLQELLQNHSFVGSVSPQWTLVQHQTKLYLLNTTKLSQELFYQILVYDFGNFGVLRLSNPAPLYDLAMLALDSEESGWTEEDGPKEGLAQYIVDFLKQKAEMLEEYFSLEIDAEGNLTGLPMLLDNYTPAMEGLPMFILRLATEVNWDKEKECFREFSVECSHFYSIRKSYTLEADADEPQDAEMSWQWKVEHVLFKALRSLFSPAKHLSEDGSVLQIASLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_009307549.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307549.2

ORF Insert Sequence:

ATGGAAATAG TCTGTGAGCG TTTTACGACC AGCAAGCTGC AGTCTTTTGA AGATTTGTCA 
TCCATCGCAA CATACGGATT CAGAGGAGAA GCCCTCGCCA GTATAAGTCA TGTAGCTCAC 
GTCACCATCA CCACAAAAAC AGCAGATGCT AAATGTGCTT ACAGGGCCAA TTACTGTGAT 
GGGAAACTAA AATCTCCACC CAAACCCTGT GCTGGAAACC AGGGGACACT GATTTCTGTA 
GAGGATCTGT TTTATAATGT GTCTACGCGA CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA 
TATTCCAGAA TCGTCGAGGT TGTGAGCAGA TACGCCATTC ACAACTCTGG GAAAAGTTTT 
TCTGTCAAGA AGCAAGGTGA GATGGTTGCA GATGTGAAGA CTCTCCCAAA CGCCTCTGTG 
CTGGACAACA TCCGTGTGGT GTTTGGAGTC GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA 
TGCGAGGATC AGAAATTTGC TTTTAAAGTG AAGGGCTACA TCTCCAATGC TAACTACTCT 
GTCAAGAAAT GCATCCTTAT CCTTTTTATC AATCATCGAT TGGTGGAGTC TAGTGCCTTG 
AAGAAAGCAA TTGAGACCGT CTACACCGCT TATCTTCCCA AAAACACTCA TCCTTTTCTT 
TATCTCAGTT TAGAGATTGC TCCTCAAAAC ATTGATGTAA ACGTTCACCC GACAAAACAT 
GAGGTGCACT TCCTGCACGA GGACTCCATC ATCGAGAGCA TTCAGAAGCA CATCGAGAGT 
AAACTCCTCG GCTCCAATTC CTCTCGCACG TACTTCACAC AGACTCTACT TCCGGGACTT 
TCAGCATCTG CAAGCGTGGC AAAAGCTTCT AGTTCCTCAG CAGATTCCCA GGAGCGAGTT 
TACGCCCATC AGATGGTCCG CACTGACAGT AAAGCCCAGA AGCTTGATGC ATTTCTCCAG 
CCATCAGCCT CATCTTCATC AACTGCTGCT CAAAGAAAGA CAGAAAAAAC CTCCAGCACG 
TCAACTGCTG TACAAGACTT GGTGGAGCTG GACGACGCAG AGCTGCTGAC AGCTGCTGAT 
GTGGAGCCGT GTGGCGGAAA GGATCCTCAG ACTGATGCTC AACCTCCTGG TGATGAAGCA 
CCACCAAGGA AAAGGCCTCA TGTTGAGGAG GTGAAGGAGG ATTTGACAGC TGCTTCTCTT 
CCCAGGAGAC GAATCGTTAA ACTGACCAGC ATAAAGGAGC TTCGGGACCA GATTGAGCTG 
CAAACTCACA AAGGTTTACA GGAGCTGCTG CAGAATCACT CATTTGTAGG TTCAGTCAGT 
CCTCAGTGGA CTTTGGTGCA GCACCAGACC AAACTCTACC TGCTCAATAC AACCAAACTC 
AGTCAGGAGC TGTTTTATCA AATTTTAGTC TACGACTTTG GTAACTTCGG CGTTCTTCGA 
TTGTCGAATC CAGCGCCGCT CTATGATCTG GCCATGTTGG CTCTGGATTC AGAGGAAAGT 
GGATGGACAG AGGAGGATGG TCCGAAAGAA GGACTGGCCC AGTATATTGT GGATTTCCTT 
AAGCAGAAAG CAGAAATGCT GGAAGAATAC TTTTCCTTGG AAATAGATGC AGAGGGAAAC 
CTGACAGGTC TGCCAATGCT GTTGGATAAT TACACTCCTG CCATGGAGGG ACTGCCAATG 
TTCATTTTGC GTTTAGCCAC TGAGGTGAAC TGGGATAAAG AAAAGGAGTG TTTCCGTGAA 
TTCAGTGTTG AATGCAGTCA TTTCTACTCC ATAAGGAAAA GCTACACACT GGAGGCAGAC 
GCAGATGAGC CACAGGATGC TGAGATGAGC TGGCAGTGGA AGGTGGAGCA TGTGCTTTTC 
AAAGCTTTGC GCTCTCTCTT CAGTCCTGCC AAACACTTGA GTGAAGACGG CAGTGTCCTG 
CAGATCGCCA GCCTGCCTGA CCTCTACAAA GTGTTTGAAA GATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_009307549.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305824.1 Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40481
Clone ID Related Accession (Same CDS sequence)	XM_009307549.2 , XM_009307549.3
Accession Version	XM_009307549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA mismatch repair protein Mlh1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATAG TCTGTGAGCG TTTTACGACC AGCAAGCTGC AGTCTTTTGA AGATTTGTCA 
TCCATCGCAA CATACGGATT CAGAGGAGAA GCCCTCGCCA GTATAAGTCA TGTAGCTCAC 
GTCACCATCA CCACAAAAAC AGCAGATGCT AAATGTGCTT ACAGGGCCAA TTACTGTGAT 
GGGAAACTAA AATCTCCACC CAAACCCTGT GCTGGAAACC AGGGGACACT GATTTCTGTA 
GAGGATCTGT TTTATAATGT GTCTACGCGA CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA 
TATTCCAGAA TCGTCGAGGT TGTGAGCAGA TACGCCATTC ACAACTCTGG GAAAAGTTTT 
TCTGTCAAGA AGCAAGGTGA GATGGTTGCA GATGTGAAGA CTCTCCCAAA CGCCTCTGTG 
CTGGACAACA TCCGTGTGGT GTTTGGAGTC GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA 
TGCGAGGATC AGAAATTTGC TTTTAAAGTG AAGGGCTACA TCTCCAATGC TAACTACTCT 
GTCAAGAAAT GCATCCTTAT CCTTTTTATC AATCATCGAT TGGTGGAGTC TAGTGCCTTG 
AAGAAAGCAA TTGAGACCGT CTACACCGCT TATCTTCCCA AAAACACTCA TCCTTTTCTT 
TATCTCAGTT TAGAGATTGC TCCTCAAAAC ATTGATGTAA ACGTTCACCC GACAAAACAT 
GAGGTGCACT TCCTGCACGA GGACTCCATC ATCGAGAGCA TTCAGAAGCA CATCGAGAGT 
AAACTCCTCG GCTCCAATTC CTCTCGCACG TACTTCACAC AGACTCTACT TCCGGGACTT 
TCAGCATCTG CAAGCGTGGC AAAAGCTTCT AGTTCCTCAG CAGATTCCCA GGAGCGAGTT 
TACGCCCATC AGATGGTCCG CACTGACAGT AAAGCCCAGA AGCTTGATGC ATTTCTCCAG 
CCATCAGCCT CATCTTCATC AACTGCTGCT CAAAGAAAGA CAGAAAAAAC CTCCAGCACG 
TCAACTGCTG TACAAGACTT GGTGGAGCTG GACGACGCAG AGCTGCTGAC AGCTGCTGAT 
GTGGAGCCGT GTGGCGGAAA GGATCCTCAG ACTGATGCTC AACCTCCTGG TGATGAAGCA 
CCACCAAGGA AAAGGCCTCA TGTTGAGGAG GTGAAGGAGG ATTTGACAGC TGCTTCTCTT 
CCCAGGAGAC GAATCGTTAA ACTGACCAGC ATAAAGGAGC TTCGGGACCA GATTGAGCTG 
CAAACTCACA AAGGTTTACA GGAGCTGCTG CAGAATCACT CATTTGTAGG TTCAGTCAGT 
CCTCAGTGGA CTTTGGTGCA GCACCAGACC AAACTCTACC TGCTCAATAC AACCAAACTC 
AGTCAGGAGC TGTTTTATCA AATTTTAGTC TACGACTTTG GTAACTTCGG CGTTCTTCGA 
TTGTCGAATC CAGCGCCGCT CTATGATCTG GCCATGTTGG CTCTGGATTC AGAGGAAAGT 
GGATGGACAG AGGAGGATGG TCCGAAAGAA GGACTGGCCC AGTATATTGT GGATTTCCTT 
AAGCAGAAAG CAGAAATGCT GGAAGAATAC TTTTCCTTGG AAATAGATGC AGAGGGAAAC 
CTGACAGGTC TGCCAATGCT GTTGGATAAT TACACTCCTG CCATGGAGGG ACTGCCAATG 
TTCATTTTGC GTTTAGCCAC TGAGGTGAAC TGGGATAAAG AAAAGGAGTG TTTCCGTGAA 
TTCAGTGTTG AATGCAGTCA TTTCTACTCC ATAAGGAAAA GCTACACACT GGAGGCAGAC 
GCAGATGAGC CACAGGATGC TGAGATGAGC TGGCAGTGGA AGGTGGAGCA TGTGCTTTTC 
AAAGCTTTGC GCTCTCTCTT CAGTCCTGCC AAACACTTGA GTGAAGACGG CAGTGTCCTG 
CAGATCGCCA GCCTGCCTGA CCTCTACAAA GTGTTTGAAA GATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305824.1
CDS	131..2098
Translation	MEIVCERFTTSKLQSFEDLSSIATYGFRGEALASISHVAHVTITTKTADAKCAYRANYCDGKLKSPPKPCAGNQGTLISVEDLFYNVSTRRKALKSPSEEYSRIVEVVSRYAIHNSGKSFSVKKQGEMVADVKTLPNASVLDNIRVVFGVAVSRELIEVECEDQKFAFKVKGYISNANYSVKKCILILFINHRLVESSALKKAIETVYTAYLPKNTHPFLYLSLEIAPQNIDVNVHPTKHEVHFLHEDSIIESIQKHIESKLLGSNSSRTYFTQTLLPGLSASASVAKASSSSADSQERVYAHQMVRTDSKAQKLDAFLQPSASSSSTAAQRKTEKTSSTSTAVQDLVELDDAELLTAADVEPCGGKDPQTDAQPPGDEAPPRKRPHVEEVKEDLTAASLPRRRIVKLTSIKELRDQIELQTHKGLQELLQNHSFVGSVSPQWTLVQHQTKLYLLNTTKLSQELFYQILVYDFGNFGVLRLSNPAPLYDLAMLALDSEESGWTEEDGPKEGLAQYIVDFLKQKAEMLEEYFSLEIDAEGNLTGLPMLLDNYTPAMEGLPMFILRLATEVNWDKEKECFREFSVECSHFYSIRKSYTLEADADEPQDAEMSWQWKVEHVLFKALRSLFSPAKHLSEDGSVLQIASLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_009307549.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307549.3

ORF Insert Sequence:

ATGGAAATAG TCTGTGAGCG TTTTACGACC AGCAAGCTGC AGTCTTTTGA AGATTTGTCA 
TCCATCGCAA CATACGGATT CAGAGGAGAA GCCCTCGCCA GTATAAGTCA TGTAGCTCAC 
GTCACCATCA CCACAAAAAC AGCAGATGCT AAATGTGCTT ACAGGGCCAA TTACTGTGAT 
GGGAAACTAA AATCTCCACC CAAACCCTGT GCTGGAAACC AGGGGACACT GATTTCTGTA 
GAGGATCTGT TTTATAATGT GTCTACGCGA CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA 
TATTCCAGAA TCGTCGAGGT TGTGAGCAGA TACGCCATTC ACAACTCTGG GAAAAGTTTT 
TCTGTCAAGA AGCAAGGTGA GATGGTTGCA GATGTGAAGA CTCTCCCAAA CGCCTCTGTG 
CTGGACAACA TCCGTGTGGT GTTTGGAGTC GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA 
TGCGAGGATC AGAAATTTGC TTTTAAAGTG AAGGGCTACA TCTCCAATGC TAACTACTCT 
GTCAAGAAAT GCATCCTTAT CCTTTTTATC AATCATCGAT TGGTGGAGTC TAGTGCCTTG 
AAGAAAGCAA TTGAGACCGT CTACACCGCT TATCTTCCCA AAAACACTCA TCCTTTTCTT 
TATCTCAGTT TAGAGATTGC TCCTCAAAAC ATTGATGTAA ACGTTCACCC GACAAAACAT 
GAGGTGCACT TCCTGCACGA GGACTCCATC ATCGAGAGCA TTCAGAAGCA CATCGAGAGT 
AAACTCCTCG GCTCCAATTC CTCTCGCACG TACTTCACAC AGACTCTACT TCCGGGACTT 
TCAGCATCTG CAAGCGTGGC AAAAGCTTCT AGTTCCTCAG CAGATTCCCA GGAGCGAGTT 
TACGCCCATC AGATGGTCCG CACTGACAGT AAAGCCCAGA AGCTTGATGC ATTTCTCCAG 
CCATCAGCCT CATCTTCATC AACTGCTGCT CAAAGAAAGA CAGAAAAAAC CTCCAGCACG 
TCAACTGCTG TACAAGACTT GGTGGAGCTG GACGACGCAG AGCTGCTGAC AGCTGCTGAT 
GTGGAGCCGT GTGGCGGAAA GGATCCTCAG ACTGATGCTC AACCTCCTGG TGATGAAGCA 
CCACCAAGGA AAAGGCCTCA TGTTGAGGAG GTGAAGGAGG ATTTGACAGC TGCTTCTCTT 
CCCAGGAGAC GAATCGTTAA ACTGACCAGC ATAAAGGAGC TTCGGGACCA GATTGAGCTG 
CAAACTCACA AAGGTTTACA GGAGCTGCTG CAGAATCACT CATTTGTAGG TTCAGTCAGT 
CCTCAGTGGA CTTTGGTGCA GCACCAGACC AAACTCTACC TGCTCAATAC AACCAAACTC 
AGTCAGGAGC TGTTTTATCA AATTTTAGTC TACGACTTTG GTAACTTCGG CGTTCTTCGA 
TTGTCGAATC CAGCGCCGCT CTATGATCTG GCCATGTTGG CTCTGGATTC AGAGGAAAGT 
GGATGGACAG AGGAGGATGG TCCGAAAGAA GGACTGGCCC AGTATATTGT GGATTTCCTT 
AAGCAGAAAG CAGAAATGCT GGAAGAATAC TTTTCCTTGG AAATAGATGC AGAGGGAAAC 
CTGACAGGTC TGCCAATGCT GTTGGATAAT TACACTCCTG CCATGGAGGG ACTGCCAATG 
TTCATTTTGC GTTTAGCCAC TGAGGTGAAC TGGGATAAAG AAAAGGAGTG TTTCCGTGAA 
TTCAGTGTTG AATGCAGTCA TTTCTACTCC ATAAGGAAAA GCTACACACT GGAGGCAGAC 
GCAGATGAGC CACAGGATGC TGAGATGAGC TGGCAGTGGA AGGTGGAGCA TGTGCTTTTC 
AAAGCTTTGC GCTCTCTCTT CAGTCCTGCC AAACACTTGA GTGAAGACGG CAGTGTCCTG 
CAGATCGCCA GCCTGCCTGA CCTCTACAAA GTGTTTGAAA GATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_009307550.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305825.1 Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X2, mRNA.

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CloneID	ODa40482
Clone ID Related Accession (Same CDS sequence)	XM_009307550.2
Accession Version	XM_009307550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA mismatch repair protein Mlh1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG GAGTCATCCG CAGACTCGAC GAGACTGTAG TGAACCGCAT CGCAGCCGGA 
GAGATTATCC AGCGGCCTGC CAATGCTATC AAAGAGATGA TGGAGAACTG TTTGGATGCA 
AAGTCTACGA ACATCCAGAT AACAGTGAAA GAGGGCGGAC TGAAACTCAT CCTTATTCAG 
GACAATGGCA CTGGAATTAG AAAAGATGAT ATGGAAATAG TCTGTGAGCG TTTTACGACC 
AGCAAGCTGC AGTCTTTTGA AGATTTGTCA TCCATCGCAA CATACGGATT CAGAGGAGAA 
GCCCTCGCCA GTATAAGTCA TGTAGCTCAC GTCACCATCA CCACAAAAAC AGCAGATGCT 
AAATGTGCTT ACAGGGCCAA TTACTGTGAT GGGAAACTAA AATCTCCACC CAAACCCTGT 
GCTGGAAACC AGGGGACACT GATTTCTGTA GAGGATCTGT TTTATAATGT GTCTACGCGA 
CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA TATTCCAGAA TCGTCGAGGT TGTGAGCAGA 
TACGCCATTC ACAACTCTGG GAAAAGTTTT TCTGTCAAGA AGCAAGGTGA GATGGTTGCA 
GATGTGAAGA CTCTCCCAAA CGCCTCTGTG CTGGACAACA TCCGTGTGGT GTTTGGAGTC 
GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA TGCGAGGATC AGAAATTTGC TTTTAAAGTG 
AAGGGCTACA TCTCCAATGC TAACTACTCT GTCAAGAAAT GCATCCTTAT CCTTTTTATC 
AATCATCGAT TGGTGGAGTC TAGTGCCTTG AAGAAAGCAA TTGAGACCGT CTACACCGCT 
TATCTTCCCA AAAACACTCA TCCTTTTCTT TATCTCAGAC TCTACTTCCG GGACTTTCAG 
CATCTGCAAG CGTGGCAAAA GCTTCTAGTT CCTCAGCAGA TTCCCAGGAG CGAGTTTACG 
CCCATCAGAT GGTCCGCACT GACAGTAAAG CCCAGAAGCT TGATGCATTT CTCCAGCCAT 
CAGCCTCATC TTCATCAACT GCTGCTCAAA GAAAGACAGA AAAAACCTCC AGCACGTCAA 
CTGCTGTACA AGACTTGGTG GAGCTGGACG ACGCAGAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305825.1
CDS	154..1278
Translation	MMAGVIRRLDETVVNRIAAGEIIQRPANAIKEMMENCLDAKSTNIQITVKEGGLKLILIQDNGTGIRKDDMEIVCERFTTSKLQSFEDLSSIATYGFRGEALASISHVAHVTITTKTADAKCAYRANYCDGKLKSPPKPCAGNQGTLISVEDLFYNVSTRRKALKSPSEEYSRIVEVVSRYAIHNSGKSFSVKKQGEMVADVKTLPNASVLDNIRVVFGVAVSRELIEVECEDQKFAFKVKGYISNANYSVKKCILILFINHRLVESSALKKAIETVYTAYLPKNTHPFLYLRLYFRDFQHLQAWQKLLVPQQIPRSEFTPIRWSALTVKPRSLMHFSSHQPHLHQLLLKERQKKPPARQLLYKTWWSWTTQSC

Target ORF information:

RefSeq Version	XM_009307550.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307550.2

ORF Insert Sequence:

ATGATGGCGG GAGTCATCCG CAGACTCGAC GAGACTGTAG TGAACCGCAT CGCAGCCGGA 
GAGATTATCC AGCGGCCTGC CAATGCTATC AAAGAGATGA TGGAGAACTG TTTGGATGCA 
AAGTCTACGA ACATCCAGAT AACAGTGAAA GAGGGCGGAC TGAAACTCAT CCTTATTCAG 
GACAATGGCA CTGGAATTAG AAAAGATGAT ATGGAAATAG TCTGTGAGCG TTTTACGACC 
AGCAAGCTGC AGTCTTTTGA AGATTTGTCA TCCATCGCAA CATACGGATT CAGAGGAGAA 
GCCCTCGCCA GTATAAGTCA TGTAGCTCAC GTCACCATCA CCACAAAAAC AGCAGATGCT 
AAATGTGCTT ACAGGGCCAA TTACTGTGAT GGGAAACTAA AATCTCCACC CAAACCCTGT 
GCTGGAAACC AGGGGACACT GATTTCTGTA GAGGATCTGT TTTATAATGT GTCTACGCGA 
CGGAAAGCCC TAAAGAGCCC CAGTGAGGAA TATTCCAGAA TCGTCGAGGT TGTGAGCAGA 
TACGCCATTC ACAACTCTGG GAAAAGTTTT TCTGTCAAGA AGCAAGGTGA GATGGTTGCA 
GATGTGAAGA CTCTCCCAAA CGCCTCTGTG CTGGACAACA TCCGTGTGGT GTTTGGAGTC 
GCAGTTAGCA GGGAGCTGAT TGAAGTTGAA TGCGAGGATC AGAAATTTGC TTTTAAAGTG 
AAGGGCTACA TCTCCAATGC TAACTACTCT GTCAAGAAAT GCATCCTTAT CCTTTTTATC 
AATCATCGAT TGGTGGAGTC TAGTGCCTTG AAGAAAGCAA TTGAGACCGT CTACACCGCT 
TATCTTCCCA AAAACACTCA TCCTTTTCTT TATCTCAGAC TCTACTTCCG GGACTTTCAG 
CATCTGCAAG CGTGGCAAAA GCTTCTAGTT CCTCAGCAGA TTCCCAGGAG CGAGTTTACG 
CCCATCAGAT GGTCCGCACT GACAGTAAAG CCCAGAAGCT TGATGCATTT CTCCAGCCAT 
CAGCCTCATC TTCATCAACT GCTGCTCAAA GAAAGACAGA AAAAACCTCC AGCACGTCAA 
CTGCTGTACA AGACTTGGTG GAGCTGGACG ACGCAGAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( NM_200659.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_956953.1 Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), mRNA.

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CloneID	ODa35042
Clone ID Related Accession (Same CDS sequence)	NM_200659.1
Accession Version	NM_200659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA mismatch repair protein Mlh1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057507.1. ##Evidence-Data-START## Transcript exon combination :: BC057507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGAG TCATCCGCAG ACTCGACGAG ACTGTAGTGA ACCGCATCGC AGCCGGAGAG 
ATTATCCAGC GGCCTGCCAA TGCTATCAAA GAGATGATGG AGAACTGTTT GGATGCAAAG 
TCTACGAACA TCCAGATAAC AGTGAAAGAG GGCGGACTGA AACTCATCCT TATTCAGGAC 
AATGGCACTG GAATTAGAAA AGATGATATG GAAATAGTCT GTGAGCGTTT TACGACCAGC 
AAGCTGAAGT CTTTTGACGA TTTGTCATCC ATCGCAACAT ACGGATTCAG AGGAGAAGCC 
CTCGCCAGTA TAAGTCATGT AGCTCACGTC ACCATCACCA CAAAAACAGC AGATGCTAAA 
TGTGCTTACA GGGCCAATTA CTGTGATGGG AAACTAAAAT CTCCACCCAA ACCCTGTGCT 
GGAAACCAGG GGACACTGAT TTCTGTAGAG GATCTGTTTT ATAATGTGTC TACGCGACGG 
AAAGCCCTAA AGAGCCCCAG TGAGGAATAT TCCAGAATCG TCGAGGTTGT GAGCAGATAC 
GCCATTCACA ACTCTGGGAA AAGTTTTTCT GTCAAGAAGC AAGGTGAGAT GGTTGCAGAT 
GTGAAGACTC TCCCAAACGC CTCTGTGCTG GACAACATCC GTGTGGTGTT TGGAGTCGCA 
GTTAGCAGGG AGCTGATTGA AGTTGAATGC GAGGATCAGA AATTTGCTTT TAAAGTGAAG 
GGCTACATCT CCAATGCTAA CTACTCTGTC AAGAAATGCA TCCTTATCCT TTTTATCAAT 
CATCGATTGG TGGAGTCTAG TGCCTTGAAG AAAGCAATTG AGACCGTCTA CACCGCTTAT 
CTTCCCAAAA ACACTCATCC TTTTCTTTAT CTCAGTTTAG AGATTGCTCC TCAAAACATT 
GATGTAAACG TTCACCCGAC AAAACATGAG GTGCACTTCC TGCACGAGGA CTCCATCATC 
GAGAGCATTC AGAAGCACAT CGAGAATAAA CTTCTCGGCT CCAATTCCTC TCGCACGTAC 
TTCACACAGA CTCTACTTCC GGGACTTTCA GCATCTGCAA GCGTGGCGAA AGCTTCTAGT 
TCCTCAGCAG ATCCCCAGGA GCGAGTTTAC GCCCATCAGA TGGTCCGCAC TGACAGTAAA 
GCCCAGAAGC TTGATGCATT TCTCCAGCCA TCAGCCTCAT CTTCATCATC TGCTGCTCAA 
AGAAAGACAG AAAAAACCTC CAGCACGTCA ACTGCTGTAC AAGACTCGGT GGAGCTGGAC 
GACGCAGAGC TGCTGACAGC TGCTGATGTG GAGCCGTGTG GGGGAGAGGA TCCCCAGACT 
GATGCTCAAC CTCCTGGTGA TGAAGCACCA CCAAGGAAAA GGCCTCATGT TGAGGAGGTG 
AAGGAGGATT TGACAGCTGC TTCTCTTCCC AGGAGACGAA TCGTTAAACT GACCAGCATA 
AAGGGGCTTC GGGACCAGAT TGAGCTGCAA ACTCACAAAG GTTTACAGGA GCTGCTGCAG 
AATCACTCAT TTGTAGGTTC AGTCAGTCCT CAGTGGACTT TGGTGCAGCA CCAGACCAAA 
CTCTACCTGC TCAATACAAC CAAACTCAGT CAGGAGCTGT TTTATCAAAT CTTGATCTAC 
GACTTTGGTA ACTTCGGCGT TCTTCGATTG TCGAATCCAG CTCCGCTCTA TGATCTGGCC 
ATGTTGGCTC TGGATTCAGA GGAAAGTGGA TGGACAGAGG AGGATGGTCC GAAAGAAGGA 
CTGGCCCAGT ATATTGTGGA TTTCCTTAAG CAGAAAGCAG AAATGCTGGA AGAATACTTT 
TCCTTGGAAA TAGATGCAGA GGGAAACCTG ACAGGTCTGC CAATGCTGTT GGATAATTAC 
ACTCCTGCCA TGGAGGGACT GCCAATGTTC ATTTTGCGTT TAGCCACTGA GGTGAACTGG 
GATAAAGAAA AGGAGTGTTT CCGTGAATTC AGTGTTGAAT GCAGTCATTT CTACTCCATA 
AGGAAAAGCT ACACACTGGA GGCAGACGCA GACGAGCCAC AGGATGCTGA GATGAGCTGG 
CAGTGGAAGG TGGAGCATGT GCTTTTCAAA GCTTTGCGCT CTCTCTTCAG TCCTGCCAAA 
CACTTGAGTG AAGACGGCAG TGTCCTGCAG ATCGCCAGCC TGCCTGACCT CTACAAAGTG 
TTTGAAAGAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956953.1
CDS	54..2228
Translation	MAGVIRRLDETVVNRIAAGEIIQRPANAIKEMMENCLDAKSTNIQITVKEGGLKLILIQDNGTGIRKDDMEIVCERFTTSKLKSFDDLSSIATYGFRGEALASISHVAHVTITTKTADAKCAYRANYCDGKLKSPPKPCAGNQGTLISVEDLFYNVSTRRKALKSPSEEYSRIVEVVSRYAIHNSGKSFSVKKQGEMVADVKTLPNASVLDNIRVVFGVAVSRELIEVECEDQKFAFKVKGYISNANYSVKKCILILFINHRLVESSALKKAIETVYTAYLPKNTHPFLYLSLEIAPQNIDVNVHPTKHEVHFLHEDSIIESIQKHIENKLLGSNSSRTYFTQTLLPGLSASASVAKASSSSADPQERVYAHQMVRTDSKAQKLDAFLQPSASSSSSAAQRKTEKTSSTSTAVQDSVELDDAELLTAADVEPCGGEDPQTDAQPPGDEAPPRKRPHVEEVKEDLTAASLPRRRIVKLTSIKGLRDQIELQTHKGLQELLQNHSFVGSVSPQWTLVQHQTKLYLLNTTKLSQELFYQILIYDFGNFGVLRLSNPAPLYDLAMLALDSEESGWTEEDGPKEGLAQYIVDFLKQKAEMLEEYFSLEIDAEGNLTGLPMLLDNYTPAMEGLPMFILRLATEVNWDKEKECFREFSVECSHFYSIRKSYTLEADADEPQDAEMSWQWKVEHVLFKALRSLFSPAKHLSEDGSVLQIASLPDLYKVFERC

Target ORF information:

RefSeq Version	NM_200659.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (mlh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200659.1

ORF Insert Sequence:

ATGGCGGGAG TCATCCGCAG ACTCGACGAG ACTGTAGTGA ACCGCATCGC AGCCGGAGAG 
ATTATCCAGC GGCCTGCCAA TGCTATCAAA GAGATGATGG AGAACTGTTT GGATGCAAAG 
TCTACGAACA TCCAGATAAC AGTGAAAGAG GGCGGACTGA AACTCATCCT TATTCAGGAC 
AATGGCACTG GAATTAGAAA AGATGATATG GAAATAGTCT GTGAGCGTTT TACGACCAGC 
AAGCTGAAGT CTTTTGACGA TTTGTCATCC ATCGCAACAT ACGGATTCAG AGGAGAAGCC 
CTCGCCAGTA TAAGTCATGT AGCTCACGTC ACCATCACCA CAAAAACAGC AGATGCTAAA 
TGTGCTTACA GGGCCAATTA CTGTGATGGG AAACTAAAAT CTCCACCCAA ACCCTGTGCT 
GGAAACCAGG GGACACTGAT TTCTGTAGAG GATCTGTTTT ATAATGTGTC TACGCGACGG 
AAAGCCCTAA AGAGCCCCAG TGAGGAATAT TCCAGAATCG TCGAGGTTGT GAGCAGATAC 
GCCATTCACA ACTCTGGGAA AAGTTTTTCT GTCAAGAAGC AAGGTGAGAT GGTTGCAGAT 
GTGAAGACTC TCCCAAACGC CTCTGTGCTG GACAACATCC GTGTGGTGTT TGGAGTCGCA 
GTTAGCAGGG AGCTGATTGA AGTTGAATGC GAGGATCAGA AATTTGCTTT TAAAGTGAAG 
GGCTACATCT CCAATGCTAA CTACTCTGTC AAGAAATGCA TCCTTATCCT TTTTATCAAT 
CATCGATTGG TGGAGTCTAG TGCCTTGAAG AAAGCAATTG AGACCGTCTA CACCGCTTAT 
CTTCCCAAAA ACACTCATCC TTTTCTTTAT CTCAGTTTAG AGATTGCTCC TCAAAACATT 
GATGTAAACG TTCACCCGAC AAAACATGAG GTGCACTTCC TGCACGAGGA CTCCATCATC 
GAGAGCATTC AGAAGCACAT CGAGAATAAA CTTCTCGGCT CCAATTCCTC TCGCACGTAC 
TTCACACAGA CTCTACTTCC GGGACTTTCA GCATCTGCAA GCGTGGCGAA AGCTTCTAGT 
TCCTCAGCAG ATCCCCAGGA GCGAGTTTAC GCCCATCAGA TGGTCCGCAC TGACAGTAAA 
GCCCAGAAGC TTGATGCATT TCTCCAGCCA TCAGCCTCAT CTTCATCATC TGCTGCTCAA 
AGAAAGACAG AAAAAACCTC CAGCACGTCA ACTGCTGTAC AAGACTCGGT GGAGCTGGAC 
GACGCAGAGC TGCTGACAGC TGCTGATGTG GAGCCGTGTG GGGGAGAGGA TCCCCAGACT 
GATGCTCAAC CTCCTGGTGA TGAAGCACCA CCAAGGAAAA GGCCTCATGT TGAGGAGGTG 
AAGGAGGATT TGACAGCTGC TTCTCTTCCC AGGAGACGAA TCGTTAAACT GACCAGCATA 
AAGGGGCTTC GGGACCAGAT TGAGCTGCAA ACTCACAAAG GTTTACAGGA GCTGCTGCAG 
AATCACTCAT TTGTAGGTTC AGTCAGTCCT CAGTGGACTT TGGTGCAGCA CCAGACCAAA 
CTCTACCTGC TCAATACAAC CAAACTCAGT CAGGAGCTGT TTTATCAAAT CTTGATCTAC 
GACTTTGGTA ACTTCGGCGT TCTTCGATTG TCGAATCCAG CTCCGCTCTA TGATCTGGCC 
ATGTTGGCTC TGGATTCAGA GGAAAGTGGA TGGACAGAGG AGGATGGTCC GAAAGAAGGA 
CTGGCCCAGT ATATTGTGGA TTTCCTTAAG CAGAAAGCAG AAATGCTGGA AGAATACTTT 
TCCTTGGAAA TAGATGCAGA GGGAAACCTG ACAGGTCTGC CAATGCTGTT GGATAATTAC 
ACTCCTGCCA TGGAGGGACT GCCAATGTTC ATTTTGCGTT TAGCCACTGA GGTGAACTGG 
GATAAAGAAA AGGAGTGTTT CCGTGAATTC AGTGTTGAAT GCAGTCATTT CTACTCCATA 
AGGAAAAGCT ACACACTGGA GGCAGACGCA GACGAGCCAC AGGATGCTGA GATGAGCTGG 
CAGTGGAAGG TGGAGCATGT GCTTTTCAAA GCTTTGCGCT CTCTCTTCAG TCCTGCCAAA 
CACTTGAGTG AAGACGGCAG TGTCCTGCAG ATCGCCAGCC TGCCTGACCT CTACAAAGTG 
TTTGAAAGAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetics(2017 Dec)
Xia H,Zhong C,Wu X,Chen J,Tao B,Xia X,Shi M,Zhu Z,Trudeau VL,Hu W

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Nucleic acids research45(6)3422-3436(2017 Apr)
Lei L,Yan SY,Yang R,Chen JY,Li Y,Bu Y,Chang N,Zhou Q,Zhu X,Li CY,Xiong JW

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Cell reports18(2)545-556(2017 01)
Zhang Y,O'Leary MN,Peri S,Wang M,Zha J,Melov S,Kappes DJ,Feng Q,Rhodes J,Amieux PS,Morris DR,Kennedy BK,Wiest DL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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MLH1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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