mfn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mfn1b
Entrez Gene ID	393620
Full Name	mitofusin 1b
Synonyms	mfn1,zgc:66150
General protein information	Preferred Names mitofusin 1b Names mitofusin-1 mitofusin 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200647.1	NP_956941.1	mitofusin-1
XM_017358379.2	XP_017213868.2	mitofusin-1 isoform X1

Pathways from BioSystems

Homologies

	MFN1	XP_001108853.1
Pan troglodytes (chimpanzee)	MFN1	XP_516887.2
Canis lupus familiaris (dog)	MFN1	XP_545210.2
Bos taurus (cattle)	MFN1	NP_001193437.1
Rattus norvegicus (Norway rat)	Mfn1	NP_620432.1
Gallus gallus (chicken)	MFN1	NP_001012949.1
Danio rerio (zebrafish)	mfn1b	NP_956941.1
Homo sapiens (human)	MFN1	NP_284941.2
Mus musculus (house mouse)	Mfn1	NP_077162.2
Xenopus tropicalis (tropical clawed frog)	mfn1	NP_001016189.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Garcia-Reyero N, Escalon L, Prats E, Faria M, Soares AM, Rald?a D
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)

ES1 is a mitochondrial enlarging factor contributing to form mega-mitochondria in zebrafish cones.
Masuda T, Wada Y, Kawamura S
Scientific reports622360(2016 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mfn1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfn1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36392	NM_200647.1 Latest version!	Danio rerio mitofusin 1b (mfn1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa147850	XM_017358379.2 Latest version!	Danio rerio mitofusin 1b (mfn1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mfn1b ( NM_200647.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956941.1 Danio rerio mitofusin 1b (mfn1b), mRNA.

Price & Availability↑

CloneID	ODa36392
Clone ID Related Accession (Same CDS sequence)	NM_200647.1
Accession Version	NM_200647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	mitofusin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057468.1. On Jan 14, 2011 this sequence version replaced XR_044725.2. ##Evidence-Data-START## Transcript exon combination :: BC057468.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGAGCAGT TAGATCCCTC TCCGTTAAAG CGCTTCGTTC TGGCCAAGAA GAAGATCAGC 
GAAGTGTTCG AGCAGCTCCT AGTTTACGTT CAGGAGGGTT GCGAGTTTGT AAAAGAAACC 
TGTGCCAATG AGTCGTTGGA GAATATTGCC AACAAAGACC AGTTGGATGA AATCGAATCG 
TACACCAGCA AACTGTCCAT CATTCAGGAG GTGTTGGCTC GCAGACATAT GAAGGTGGCT 
TTCTTCGGCA GAACAAGCAA TGGTAAAAGC ACCGTCATCA ACGCTATGCT GCGTGACCGT 
GTGCTGCCCA GCGGCATCGG ACACACAACT AACTGCTTTC TGAGTGTGGA GGGAACAGAT 
GAGGACAAGG CCTTTCTGAA GACTGAAGGC TCCGAGGAGG AGAAAAGCAT CAAGACAGTA 
AATCAGCTGG CTCACGCTCT GCATATGGAC GAGAGTCTGG ACGCCGGCTG TCTGGTCAAA 
GTTTTCTGGC CCAAAACGAA GTGTGCTCTG CTCAGAGACG ACCTGGTTCT GGTGGACAGT 
CCTGGAACTG ATGTGACCAC CGAGCTGGAC AGCTGGATTG ATAAGTTCTG TCTGGACGCG 
GATGTGTTTG TGTTGGTGGC GAACTCTGAA TCCACACTAA TGAACACGGA GAAGCATTTC 
TTCCACAAGG TCAATGAAAA ACTTTCCAAG CCCAACATCT TCGTCTTGAA CAATCGCTGG 
GACGCTTCTG CTGCCGAACC CGAATACATG GAGGACGTGC GCAAGCAGCA CACGGACCGG 
TGTGTGAACT TCCTGGTGGA GGAGCTGAAG GTGGTGGATC GCGCTCAGGC ACCGAACCGC 
ATTTTCTTTG TGTCGGCGAA GGAGGTGCTG AACTCGCGGA TGCAGCGGGC GCAGGGAGGC 
GCTCTCGCTG AGGGGTTTCA GGACCGACTG AAGGAGTTCC AGAACTTTGA GAGATCTTTT 
GAGGAGTGTA TCTCGCATTC AGCAGTGAAA ACAAAGTTTG AGCAGCACAC AATCAGAGCA 
AAGCAGATCA CAGAAACAGT CAAAGACATC ATGGATCAAA TCAACATCAC CGCCGCAGAA 
AAGAGGGTTG TTTCCCTGGA GGAGCGAGAG TATCTGATCG ACCGGCTGGA TTTCGTTCGC 
AATCAACTCA ACCTGCTTAT CCAGGACATC AAGAAGAAAA TCAAGGCCAT CACGGAGGAG 
GTGGAAAACA AGGTGTCAAA CGCCATGGCG GAGGAGATCT GTCGTCTGTC TGTGATCATA 
GACGAGTTTC GGTCAGATTT CCACCCGTCG CCTCAAATGC TCAAAATGTA CAAATCTGAT 
CTGCTCAGCC ATATTGAGCA GGGCATGGGC AAGAATCTGG CTTTCCGCTG TTCTGATGCT 
GTAAACGCCT CTGTTCAGTG TTCTCAGCAA GACATGATCG AGTGTCTGAA GCCTCTGCTG 
CCCTCTGCTG CCCAGACGCA GCTCCACATG CTCATCCCCA GCCGCAAGTT TGAGCTCAGC 
TACGACCTGA ACTGCGCCAC GCTCTGCTCA GACTTCCAGG AGAACATCGA GTTCCAGTTC 
TCCTTAGGCT GGTCTGCGCT GGTGCACAGA TTCCTGGGTC CCGTCAACGC CAAACGAGCC 
CTGATGCTGG TGGACCAGAA CCTACAGCTG ACAGCACCAA CCACCCCTAC AGCCCCAGCG 
GTGGTTCAGT CCCAGAACTC CAGAGCTCTG GGTCAGAATC AGGCCTCAGT GACCCAGGAG 
GAGCTCATGT TGACCATGGT GAATAACCTG GCCTCGGTCA CCTCACGCAC GTCTATGAGC 
GTGATCATTG TTGGAGGAGT GGTGTGGCGT ACAGTCGGCT GGCGTCTGAT TGCTCTCTCC 
ATGAGTATGT ATGGTCTGCT GTACCTGTAC GAAAGACTCA CCTGGACCAC CAAAGCCAAG 
GAAAGAGCCC TGAAGAAGCA GTTTGTTGAT TACGCCACCG AGAAACTGCA AATGATCATC 
AGCTTCACCA GCTCAAACTG CAGCCACCAA GTGCAGCAGG AAATCGCCAG CACGTTCGCC 
CGACTCTGCC AGCAGGTTGA CATTACCCAG AAGGACCTGG AGAGTGACAT CCAACGCCTC 
ACTGACAAGA TCCAGAAGCT GGAAACCGTA CAGAACCGAT CCAAAGTGCT CAGACATAAA 
GCCACAGCTC TGGAAATGCA GCTGGAGGAT TTCAGCTCCC AGTATTTGCG TCTTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956941.1
CDS	92..2314
Translation	MEQLDPSPLKRFVLAKKKISEVFEQLLVYVQEGCEFVKETCANESLENIANKDQLDEIESYTSKLSIIQEVLARRHMKVAFFGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDEDKAFLKTEGSEEEKSIKTVNQLAHALHMDESLDAGCLVKVFWPKTKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNEKLSKPNIFVLNNRWDASAAEPEYMEDVRKQHTDRCVNFLVEELKVVDRAQAPNRIFFVSAKEVLNSRMQRAQGGALAEGFQDRLKEFQNFERSFEECISHSAVKTKFEQHTIRAKQITETVKDIMDQINITAAEKRVVSLEEREYLIDRLDFVRNQLNLLIQDIKKKIKAITEEVENKVSNAMAEEICRLSVIIDEFRSDFHPSPQMLKMYKSDLLSHIEQGMGKNLAFRCSDAVNASVQCSQQDMIECLKPLLPSAAQTQLHMLIPSRKFELSYDLNCATLCSDFQENIEFQFSLGWSALVHRFLGPVNAKRALMLVDQNLQLTAPTTPTAPAVVQSQNSRALGQNQASVTQEELMLTMVNNLASVTSRTSMSVIIVGGVVWRTVGWRLIALSMSMYGLLYLYERLTWTTKAKERALKKQFVDYATEKLQMIISFTSSNCSHQVQQEIASTFARLCQQVDITQKDLESDIQRLTDKIQKLETVQNRSKVLRHKATALEMQLEDFSSQYLRLQP

Target ORF information:

RefSeq Version	NM_200647.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitofusin 1b (mfn1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200647.1

ORF Insert Sequence:

ATGGAGCAGT TAGATCCCTC TCCGTTAAAG CGCTTCGTTC TGGCCAAGAA GAAGATCAGC 
GAAGTGTTCG AGCAGCTCCT AGTTTACGTT CAGGAGGGTT GCGAGTTTGT AAAAGAAACC 
TGTGCCAATG AGTCGTTGGA GAATATTGCC AACAAAGACC AGTTGGATGA AATCGAATCG 
TACACCAGCA AACTGTCCAT CATTCAGGAG GTGTTGGCTC GCAGACATAT GAAGGTGGCT 
TTCTTCGGCA GAACAAGCAA TGGTAAAAGC ACCGTCATCA ACGCTATGCT GCGTGACCGT 
GTGCTGCCCA GCGGCATCGG ACACACAACT AACTGCTTTC TGAGTGTGGA GGGAACAGAT 
GAGGACAAGG CCTTTCTGAA GACTGAAGGC TCCGAGGAGG AGAAAAGCAT CAAGACAGTA 
AATCAGCTGG CTCACGCTCT GCATATGGAC GAGAGTCTGG ACGCCGGCTG TCTGGTCAAA 
GTTTTCTGGC CCAAAACGAA GTGTGCTCTG CTCAGAGACG ACCTGGTTCT GGTGGACAGT 
CCTGGAACTG ATGTGACCAC CGAGCTGGAC AGCTGGATTG ATAAGTTCTG TCTGGACGCG 
GATGTGTTTG TGTTGGTGGC GAACTCTGAA TCCACACTAA TGAACACGGA GAAGCATTTC 
TTCCACAAGG TCAATGAAAA ACTTTCCAAG CCCAACATCT TCGTCTTGAA CAATCGCTGG 
GACGCTTCTG CTGCCGAACC CGAATACATG GAGGACGTGC GCAAGCAGCA CACGGACCGG 
TGTGTGAACT TCCTGGTGGA GGAGCTGAAG GTGGTGGATC GCGCTCAGGC ACCGAACCGC 
ATTTTCTTTG TGTCGGCGAA GGAGGTGCTG AACTCGCGGA TGCAGCGGGC GCAGGGAGGC 
GCTCTCGCTG AGGGGTTTCA GGACCGACTG AAGGAGTTCC AGAACTTTGA GAGATCTTTT 
GAGGAGTGTA TCTCGCATTC AGCAGTGAAA ACAAAGTTTG AGCAGCACAC AATCAGAGCA 
AAGCAGATCA CAGAAACAGT CAAAGACATC ATGGATCAAA TCAACATCAC CGCCGCAGAA 
AAGAGGGTTG TTTCCCTGGA GGAGCGAGAG TATCTGATCG ACCGGCTGGA TTTCGTTCGC 
AATCAACTCA ACCTGCTTAT CCAGGACATC AAGAAGAAAA TCAAGGCCAT CACGGAGGAG 
GTGGAAAACA AGGTGTCAAA CGCCATGGCG GAGGAGATCT GTCGTCTGTC TGTGATCATA 
GACGAGTTTC GGTCAGATTT CCACCCGTCG CCTCAAATGC TCAAAATGTA CAAATCTGAT 
CTGCTCAGCC ATATTGAGCA GGGCATGGGC AAGAATCTGG CTTTCCGCTG TTCTGATGCT 
GTAAACGCCT CTGTTCAGTG TTCTCAGCAA GACATGATCG AGTGTCTGAA GCCTCTGCTG 
CCCTCTGCTG CCCAGACGCA GCTCCACATG CTCATCCCCA GCCGCAAGTT TGAGCTCAGC 
TACGACCTGA ACTGCGCCAC GCTCTGCTCA GACTTCCAGG AGAACATCGA GTTCCAGTTC 
TCCTTAGGCT GGTCTGCGCT GGTGCACAGA TTCCTGGGTC CCGTCAACGC CAAACGAGCC 
CTGATGCTGG TGGACCAGAA CCTACAGCTG ACAGCACCAA CCACCCCTAC AGCCCCAGCG 
GTGGTTCAGT CCCAGAACTC CAGAGCTCTG GGTCAGAATC AGGCCTCAGT GACCCAGGAG 
GAGCTCATGT TGACCATGGT GAATAACCTG GCCTCGGTCA CCTCACGCAC GTCTATGAGC 
GTGATCATTG TTGGAGGAGT GGTGTGGCGT ACAGTCGGCT GGCGTCTGAT TGCTCTCTCC 
ATGAGTATGT ATGGTCTGCT GTACCTGTAC GAAAGACTCA CCTGGACCAC CAAAGCCAAG 
GAAAGAGCCC TGAAGAAGCA GTTTGTTGAT TACGCCACCG AGAAACTGCA AATGATCATC 
AGCTTCACCA GCTCAAACTG CAGCCACCAA GTGCAGCAGG AAATCGCCAG CACGTTCGCC 
CGACTCTGCC AGCAGGTTGA CATTACCCAG AAGGACCTGG AGAGTGACAT CCAACGCCTC 
ACTGACAAGA TCCAGAAGCT GGAAACCGTA CAGAACCGAT CCAAAGTGCT CAGACATAAA 
GCCACAGCTC TGGAAATGCA GCTGGAGGAT TTCAGCTCCC AGTATTTGCG TCTTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfn1b ( XM_017358379.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213868.2 Danio rerio mitofusin 1b (mfn1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147850
Clone ID Related Accession (Same CDS sequence)	XM_017358379.2
Accession Version	XM_017358379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitofusin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGATCCCTC TCCGTTAAAG CGCTTCGTTC TGGCCAAGAA GAAGATCAGC 
GAAGTGTTCG AGCAGCTCCT AGTTTACGTT CAGGAGGGTT GCGAGTTTGT AAAAGAAACC 
TGTGCCAATG AGTCGTTGGA GAATATTGCC AACAAAGACC AGTTGGATGA AATCGAATCG 
TACACCAGCA AACTGTCCAT CATTCAGGAG GTGTTGGCTC GCAGACATAT GAAGGTGGCT 
TTCTTCGGCA GAACAAGCAA TGGTAAAAGC ACCGTCATCA ACGCTATGCT GCGTGACCGT 
GTGCTGCCCA GCGGCATCGG ACACACGACT AACTGCTTTC TGAGTGTGGA GGGAACAGAT 
GAGGACAAGG CCTTTCTGAA GACTGAAGGC TCCGAGGAGG AGAAAAGCAT CAAGACAGTA 
AATCAGCTGG CTCACGCTCT GCATATGGAC GAGAGTCTGG ACGCCGGCTG TCTGGTCAAA 
GTTTTCTGGC CCAAAACGAA GTGTGCTCTG CTCAGAGACG ACCTGGTTCT GGTGGACAGT 
CCTGGAACTG ACGTGACCAC AGAGCTGGAC AGCTGGATTG ATAAGTTCTG TCTGGACGCT 
GATGTGTTTG TGTTGGTGGC GAACTCTGAA TCCACACTAA TGAACACGGA GAAGCATTTC 
TTCCACAAGG TCAATGAAAA ACTTTCCAAG CCCAACATCT TCATCTTGAA CAATCGCTGG 
GACGCTTCTG CTGCCGAACC CGAATACATG GAGGACGTGC GCAAGCAGCA CACGGACCGG 
TGTGTGAACT TCCTGGTGGA GGAGCTGAAG GTGGTGGATC GCGCTCAGGC ACCGAACCGC 
ATTTTCTTTG TGTCGGCGAA GGAGGTGCTG AACTCGCGGA TGCAGCGGGC GCAGGGTATG 
CCAGAGACGG GAGGCGCTCT CGCTGAGGGG TTTCAGGACC GACTGAAGGA GTTCCAGAAC 
TTTGAGAGAT CCTTTGAGGA GTGTATCTCG CATTCAGCAG TGAAAACAAA GTTTGAGCAG 
CACACAATCA GAGCAAAGCA GATCACAGAA ACAGTCAAAG ACATCATGGA TCAAATCAAC 
ATCACCGCCG CAGAAAAGAG GGTTGTTTCC CTGGAGGAGC GAGAGTATCT GATCGACCGG 
CTGGATTTCG TTCGCAATCA ACTCAACCTG CTTATCCAAG ACATCAAGAA GAAAATCAAG 
GCCATCACGG AGGAGGTGGA AAACAAGGTG TCAAACGCCA TGGCGGAGGA GATCTGTCGT 
CTGTCTGTGA TCATAGACGA GTTTCGGTCA GATTTCCACC CGTCGCCTCA AATGCTCAAA 
ATGTACAAAT CTGATCTGCT CAGCCATATT GAGCAGGGCA TGGGCAAGAA TCTGGCTTTC 
CGCTGTTCTG ATGCTGTAAA CGCCTCTGTT CAGTGTTCTC AGCAAGACAT GATCGAGTGT 
CTGAAGCCTC TGCTGCCCTC TGCTGCCCAG ACGCAGCTCC ACATGCTCAT CCCCAGCCGC 
AAGTTTGAGC TCAGCTACGA CCTGAACTGC GCCACGCTCT GCTCAGACTT CCAGGAGAAC 
ATCGAGTTCC AGTTCTCCTT AGGCTGGTCT GCGCTGGTGC ACAGATTCCT CGGTCCCGTC 
AACGCCAAAC GAGCCCTGAT GCTGGTGGAC CAGAACCTAC AGCTGACAGC ACCAACCACC 
CCTACAGCCC CAGCGGTGGT TCAGTCCCAG AACTCCAGAG CTCTGGGTCA GAATCAGGCC 
TCAGTGACCC AGGAGGAGCT CATGTTGACC ATGGTGAATA ACCTGGCCTC GGTCACCTCA 
CGCACGTCTA TGAGCGTGAT CATTGTTGGA GGAGTGGTGT GGCGTACAGT CGGCTGGCGT 
CTGATTGCTC TCTCCATGAG TATGTATGGT CTGCTGTACC TGTACGAAAG ACTCACCTGG 
ACCACCAAAG CCAAGGAAAG AGCCCTGAAG AAGCAGTTTG TTGATTACGC CACCGAGAAA 
CTGCAAATGA TCATCAGCTT CACCAGCTCA AACTGCAGCC ACCAAGTGCA GCAGGAAATC 
GCCAGCACGT TCGCCCGACT CTGCCAGCAG GTTGACATTA CCCAGAGGGA CCTGGAGAGT 
GACATCCAAC GCCTCACTGA CAAGATCCAG AAGCTGGAAA CCGTACAGAA CCGATCCAAA 
GTGCTCAGAC ATAAAGCCAC AGCTCTGGAA ATGCAGCTGG AGGATTTCAG TTCCCAGTAT 
TTGCGTCTTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213868.2
CDS	152..2389
Translation	MEQLDPSPLKRFVLAKKKISEVFEQLLVYVQEGCEFVKETCANESLENIANKDQLDEIESYTSKLSIIQEVLARRHMKVAFFGRTSNGKSTVINAMLRDRVLPSGIGHTTNCFLSVEGTDEDKAFLKTEGSEEEKSIKTVNQLAHALHMDESLDAGCLVKVFWPKTKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNEKLSKPNIFILNNRWDASAAEPEYMEDVRKQHTDRCVNFLVEELKVVDRAQAPNRIFFVSAKEVLNSRMQRAQGMPETGGALAEGFQDRLKEFQNFERSFEECISHSAVKTKFEQHTIRAKQITETVKDIMDQINITAAEKRVVSLEEREYLIDRLDFVRNQLNLLIQDIKKKIKAITEEVENKVSNAMAEEICRLSVIIDEFRSDFHPSPQMLKMYKSDLLSHIEQGMGKNLAFRCSDAVNASVQCSQQDMIECLKPLLPSAAQTQLHMLIPSRKFELSYDLNCATLCSDFQENIEFQFSLGWSALVHRFLGPVNAKRALMLVDQNLQLTAPTTPTAPAVVQSQNSRALGQNQASVTQEELMLTMVNNLASVTSRTSMSVIIVGGVVWRTVGWRLIALSMSMYGLLYLYERLTWTTKAKERALKKQFVDYATEKLQMIISFTSSNCSHQVQQEIASTFARLCQQVDITQRDLESDIQRLTDKIQKLETVQNRSKVLRHKATALEMQLEDFSSQYLRLQP

Target ORF information:

RefSeq Version	XM_017358379.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitofusin 1b (mfn1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358379.2

ORF Insert Sequence:

ATGGAGCAGT TAGATCCCTC TCCGTTAAAG CGCTTCGTTC TGGCCAAGAA GAAGATCAGC 
GAAGTGTTCG AGCAGCTCCT AGTTTACGTT CAGGAGGGTT GCGAGTTTGT AAAAGAAACC 
TGTGCCAATG AGTCGTTGGA GAATATTGCC AACAAAGACC AGTTGGATGA AATCGAATCG 
TACACCAGCA AACTGTCCAT CATTCAGGAG GTGTTGGCTC GCAGACATAT GAAGGTGGCT 
TTCTTCGGCA GAACAAGCAA TGGTAAAAGC ACCGTCATCA ACGCTATGCT GCGTGACCGT 
GTGCTGCCCA GCGGCATCGG ACACACGACT AACTGCTTTC TGAGTGTGGA GGGAACAGAT 
GAGGACAAGG CCTTTCTGAA GACTGAAGGC TCCGAGGAGG AGAAAAGCAT CAAGACAGTA 
AATCAGCTGG CTCACGCTCT GCATATGGAC GAGAGTCTGG ACGCCGGCTG TCTGGTCAAA 
GTTTTCTGGC CCAAAACGAA GTGTGCTCTG CTCAGAGACG ACCTGGTTCT GGTGGACAGT 
CCTGGAACTG ACGTGACCAC AGAGCTGGAC AGCTGGATTG ATAAGTTCTG TCTGGACGCT 
GATGTGTTTG TGTTGGTGGC GAACTCTGAA TCCACACTAA TGAACACGGA GAAGCATTTC 
TTCCACAAGG TCAATGAAAA ACTTTCCAAG CCCAACATCT TCATCTTGAA CAATCGCTGG 
GACGCTTCTG CTGCCGAACC CGAATACATG GAGGACGTGC GCAAGCAGCA CACGGACCGG 
TGTGTGAACT TCCTGGTGGA GGAGCTGAAG GTGGTGGATC GCGCTCAGGC ACCGAACCGC 
ATTTTCTTTG TGTCGGCGAA GGAGGTGCTG AACTCGCGGA TGCAGCGGGC GCAGGGTATG 
CCAGAGACGG GAGGCGCTCT CGCTGAGGGG TTTCAGGACC GACTGAAGGA GTTCCAGAAC 
TTTGAGAGAT CCTTTGAGGA GTGTATCTCG CATTCAGCAG TGAAAACAAA GTTTGAGCAG 
CACACAATCA GAGCAAAGCA GATCACAGAA ACAGTCAAAG ACATCATGGA TCAAATCAAC 
ATCACCGCCG CAGAAAAGAG GGTTGTTTCC CTGGAGGAGC GAGAGTATCT GATCGACCGG 
CTGGATTTCG TTCGCAATCA ACTCAACCTG CTTATCCAAG ACATCAAGAA GAAAATCAAG 
GCCATCACGG AGGAGGTGGA AAACAAGGTG TCAAACGCCA TGGCGGAGGA GATCTGTCGT 
CTGTCTGTGA TCATAGACGA GTTTCGGTCA GATTTCCACC CGTCGCCTCA AATGCTCAAA 
ATGTACAAAT CTGATCTGCT CAGCCATATT GAGCAGGGCA TGGGCAAGAA TCTGGCTTTC 
CGCTGTTCTG ATGCTGTAAA CGCCTCTGTT CAGTGTTCTC AGCAAGACAT GATCGAGTGT 
CTGAAGCCTC TGCTGCCCTC TGCTGCCCAG ACGCAGCTCC ACATGCTCAT CCCCAGCCGC 
AAGTTTGAGC TCAGCTACGA CCTGAACTGC GCCACGCTCT GCTCAGACTT CCAGGAGAAC 
ATCGAGTTCC AGTTCTCCTT AGGCTGGTCT GCGCTGGTGC ACAGATTCCT CGGTCCCGTC 
AACGCCAAAC GAGCCCTGAT GCTGGTGGAC CAGAACCTAC AGCTGACAGC ACCAACCACC 
CCTACAGCCC CAGCGGTGGT TCAGTCCCAG AACTCCAGAG CTCTGGGTCA GAATCAGGCC 
TCAGTGACCC AGGAGGAGCT CATGTTGACC ATGGTGAATA ACCTGGCCTC GGTCACCTCA 
CGCACGTCTA TGAGCGTGAT CATTGTTGGA GGAGTGGTGT GGCGTACAGT CGGCTGGCGT 
CTGATTGCTC TCTCCATGAG TATGTATGGT CTGCTGTACC TGTACGAAAG ACTCACCTGG 
ACCACCAAAG CCAAGGAAAG AGCCCTGAAG AAGCAGTTTG TTGATTACGC CACCGAGAAA 
CTGCAAATGA TCATCAGCTT CACCAGCTCA AACTGCAGCC ACCAAGTGCA GCAGGAAATC 
GCCAGCACGT TCGCCCGACT CTGCCAGCAG GTTGACATTA CCCAGAGGGA CCTGGAGAGT 
GACATCCAAC GCCTCACTGA CAAGATCCAG AAGCTGGAAA CCGTACAGAA CCGATCCAAA 
GTGCTCAGAC ATAAAGCCAC AGCTCTGGAA ATGCAGCTGG AGGATTTCAG TTCCCAGTAT 
TTGCGTCTTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)
Garcia-Reyero N,Escalon L,Prats E,Faria M,Soares AM,Rald?a D

ES1 is a mitochondrial enlarging factor contributing to form mega-mitochondria in zebrafish cones.
Scientific reports622360(2016 Mar)
Masuda T,Wada Y,Kawamura S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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mfn1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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