ddb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddb1
Entrez Gene ID	393599
Full Name	damage-specific DNA binding protein 1
Synonyms	zgc:63840
General protein information	Preferred Names damage-specific DNA binding protein 1 Names DNA damage-binding protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200626.1	NP_956920.1	DNA damage-binding protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddb1	NP_056550.1
Rattus norvegicus (Norway rat)	Ddb1	NP_741992.2
Danio rerio (zebrafish)	ddb1	NP_956920.1
Xenopus tropicalis (tropical clawed frog)	ddb1	XP_002937687.2
	DDB1	XP_001082958.2
Canis lupus familiaris (dog)	DDB1	XP_533275.2
Drosophila melanogaster (fruit fly)	pic	NP_650257.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002472	XP_312466.2
Oryza sativa (rice)	Os05g0592400	NP_001056495.1
Homo sapiens (human)	DDB1	NP_001914.3
Pan troglodytes (chimpanzee)	DDB1	XP_508472.4
Bos taurus (cattle)	DDB1	NP_001073731.1
Gallus gallus (chicken)	DDB1	NP_989547.1
Arabidopsis thaliana (thale cress)	DDB1A	NP_192451.1
Arabidopsis thaliana (thale cress)	DDB1B	NP_193842.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Loss of DDB1 Leads to Transcriptional p53 Pathway Activation in Proliferating Cells, Cell Cycle Deregulation, and Apoptosis in Zebrafish Embryos.
Hu Z, Holzschuh J, Driever W
PloS one10(7)e0134299(2015)

Proteome dynamics in neutrophils of adult zebrafish upon chemically-induced inflammation.
Singh SK, Aravamudhan S, Armant O, Kr?ger M, Grabher C
Fish & shellfish immunology40(1)217-24(2014 Sep)

Selection of reliable biomarkers from PCR array analyses using relative distance computational model: methodology and proof-of-concept study.
Liu C, Xu H, Lam SH, Gong Z
PloS one8(12)e83954(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03032	NM_200626.1 Latest version!	Danio rerio damage-specific DNA binding protein 1 (ddb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddb1 ( NM_200626.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956920.1 Danio rerio damage-specific DNA binding protein 1 (ddb1), mRNA.

Price & Availability↑

CloneID	ODa03032
Clone ID Related Accession (Same CDS sequence)	NM_200626.1
Accession Version	NM_200626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	DNA damage-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056837.1. ##Evidence-Data-START## Transcript exon combination :: BC056837.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTACA ACTACGTGGT GACCGCGCAG AAGCCCACGG CGGTGAACGC CTGCATCACG 
GGCCACTTCA CCTCCGCCGA GGACCTGAAC CTGCTCATCG CCAAGAACAC GCGGCTGGAG 
ATCTACGCGG TGACGGCGGA GGGGCTGCGG CCGGTCAAAG AGGTCGGCAT GTACGGCAAG 
ATCGCGGTGA TGGAGCTGTT CAGGCCCAAG GGAGAGAGCA AAGACCTTCT GTTCATTCTG 
ACGGCCAAAT ACAACGCCTG CATCCTGGAG TACAAGCAGA GCGGAGACAG CATCGACATC 
ATCACCCGAG CCCACGGCAA CGTGCAGGAC CGCATTGGCC GGCCGTCGGA GACCGGGATC 
ATCGGCATCG TGGACCCAGA GTGCCGCATG ATCGGGCTGC GGCTGTACGA CGGGCTGTTC 
AAGGTGATCC CGCTGGACCG AGAGAACCGG GAGCTCAAGG CCTTCAACAT CCGGCTGGAG 
GAGCTGCAGG TCATCGATGT CCAGTTCCTG TACGGCTGCC AGGCCCCCAC CGTCTGCTTC 
ATATACCAGG ACCCGCAGGG CCGACACGTG AAGACATACG AGGTTTCCCT GCGGGAGAAG 
GAGTTCAATA AAGGGCCCTG GAAGCAGGAG AACGTGGAGG CCGAGGCATC GATGGTCATT 
CCAGTCCCGG AGCCGTTCGG CGGAGCCATC ATCATCGGGC AGGAGTCCAT CACCTACCAC 
AATGGAGATA AATACCTGGC CGTCGCTCCG CCCATTATCA AGCAAAGCAC CATCGTGTGC 
CACAACCGTG TGGACCCGAA CGGCTCGCGG TACCTGCTGG GCGATATGGA GGGCCGGCTG 
TTCATGCTGC TGCTAGAGAA GGAGGAGCTG ATGGACGGAG CCGTGGTGCT GAAGGACCTG 
CATGTGGAGC TGCTGGGAGA GACGTCCATC GCCGAGTGTT TGACCTATCT GGATAACGGG 
GTGGTGTTTG TGGGATCCAG ACTGGGAGAC TCTCAGCTGG TGAAGCTGAA CGTGGACAGT 
AATGACCAGG GCTCGTATGT GGGGGTGATG GAGACCTTCA CTAACCTGGG CCCAATAGTG 
GACATGTGTG TGGTGGACCT GGAGCGGCAA GGGCAAGGCC AGTTGGTGAC GTGCTCCGGA 
GCGTTTAAGG AAGGCTCTCT CAGGATCATC CGGAACGGTA TTGGGATCCA CGAGCACGCC 
AGCATCGACC TGCCGGGAAT CAAAGGTCTG TGGCCTCTTC GCTCAGAGTC CAGCAGAGAC 
ACTGATGATA TGCTGGTTTT GTCTTTCGTT GGTCAGACCA GGGTGCTGAT GCTGAGTGGA 
GAGGAGGTGG AGGAGACGGA GCTGCAGGGG TTTGTGGATA ACCAGCAGAC CTTCTTCTGT 
GGGAATGTGG CGCATCAGCA GCTCATACAG ATCACCTCGG TGTCGGTGCG GCTGGTCACT 
CAGGACAGTA AGGCTCTAGT CAGCGAGTGG AAGGAGCCGC AGGGCAGGAA CATCAGCGTG 
GCGTCCTGCA ACAACACACA GGTGGTGCTG GCGGTGGGCA GAGTGCTGTA CTACCTGCAG 
ATCCTGAGCG GAGAACTCAA GCAGATCAGC TCTACAGAGA TGGAGCATGA GGTGGCGTGT 
CTGGACATCA CTCCTCTGGG CGAGCGCACT GCTGACTCCT GCATCTGTGC TGTGGGGCTC 
TGGACCGACA TCTCCGCCCG CCTGCTCAAG CTGCCCTGCT TCACCCCTCT GCACAAGGAG 
ATGCTGGGCG GAGAGATCAT CCCTCGCTCC ATCCTCATGA CCACCTTCGA GGGCAGCCAC 
TACCTGCTGT GTGCGCTGGG AGACGGAGCG CTCTTCTACT TCGGCCTGGA TATTCAGACA 
GGTGTTCTGA GCGAGCGTAA GAAGGTGACT CTCGGCACTC AGCCCACCGT CCTGCGCACG 
TTCCGCTCGC TTTCCACCTC CAACGTGTTC GCCTGCTCCG ACCGGCCCAC CGTCATCTAC 
TCCAGCAACC ACAAGCTGGT GTTCTCCAAC GTCAACCTGA AGGAGGTCAA CTACATGTGC 
CCGCTCAACT CTGAGGGCTA CCCGGACAGT CTGGCTCTGG CCAATAACAG CACTCTCACC 
ATCGGCACCA TTGACGAGAT CCAGAAGCTC CACATCCGGA CTGTGCCGCT CTACGAGTCC 
CCCAAGCGGA TCTGTTATCA GGAGGTGTCT CAGTGTTTTG GGGTTTTGTC CAGTAGAGTT 
GAAATGCAGG ACGCCAGTGG AACCACAGCA GCCGTACGGC CCAGCGCCAG CACACAGGCT 
CTGTCCAGCA GTGTGAGCTC CAGTAAGCTG CTCCCCAGCA GCACGTCGCC CCATGAGACG 
TCATTCGGGG AGGAGGTGGA GGTGCACAGC TTACTGGTGG TGGACCAGCA CACGTTTGAG 
GTGCTGCACG CTCATCAGTT CCTGCAGAAC GAGTACGCCC TCAGCATGGT GTCCTGCAAA 
CTGGGTCGAG ATCCTGCTGT TTACTTCATC GTCGGCACGG CGATGGTTTA CCCAGAGGAG 
GCGGAGCCAA AGCAGGGCCG CATCATCGTC TTCCACTATA CTGACGGCAA ACTGCAGACG 
GTGGCGGAGA AGGAGGTGAA GGGTGCCGTT TACTCCATGG TGGAGTTCAA TGGTAAACTG 
CTGGCCAGCA TCAACAGCAC GGTGCGTCTG TACGAGTGGA CCGCAGAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956920.1
CDS	65..2758
Translation	MSYNYVVTAQKPTAVNACITGHFTSAEDLNLLIAKNTRLEIYAVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGDSIDIITRAHGNVQDRIGRPSETGIIGIVDPECRMIGLRLYDGLFKVIPLDRENRELKAFNIRLEELQVIDVQFLYGCQAPTVCFIYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIPVPEPFGGAIIIGQESITYHNGDKYLAVAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEELMDGAVVLKDLHVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNDQGSYVGVMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSESSRDTDDMLVLSFVGQTRVLMLSGEEVEETELQGFVDNQQTFFCGNVAHQQLIQITSVSVRLVTQDSKALVSEWKEPQGRNISVASCNNTQVVLAVGRVLYYLQILSGELKQISSTEMEHEVACLDITPLGERTADSCICAVGLWTDISARLLKLPCFTPLHKEMLGGEIIPRSILMTTFEGSHYLLCALGDGALFYFGLDIQTGVLSERKKVTLGTQPTVLRTFRSLSTSNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSEGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPKRICYQEVSQCFGVLSSRVEMQDASGTTAAVRPSASTQALSSSVSSSKLLPSSTSPHETSFGEEVEVHSLLVVDQHTFEVLHAHQFLQNEYALSMVSCKLGRDPAVYFIVGTAMVYPEEAEPKQGRIIVFHYTDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTAEK

Target ORF information:

RefSeq Version	NM_200626.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio damage-specific DNA binding protein 1 (ddb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200626.1

ORF Insert Sequence:

ATGTCCTACA ACTACGTGGT GACCGCGCAG AAGCCCACGG CGGTGAACGC CTGCATCACG 
GGCCACTTCA CCTCCGCCGA GGACCTGAAC CTGCTCATCG CCAAGAACAC GCGGCTGGAG 
ATCTACGCGG TGACGGCGGA GGGGCTGCGG CCGGTCAAAG AGGTCGGCAT GTACGGCAAG 
ATCGCGGTGA TGGAGCTGTT CAGGCCCAAG GGAGAGAGCA AAGACCTTCT GTTCATTCTG 
ACGGCCAAAT ACAACGCCTG CATCCTGGAG TACAAGCAGA GCGGAGACAG CATCGACATC 
ATCACCCGAG CCCACGGCAA CGTGCAGGAC CGCATTGGCC GGCCGTCGGA GACCGGGATC 
ATCGGCATCG TGGACCCAGA GTGCCGCATG ATCGGGCTGC GGCTGTACGA CGGGCTGTTC 
AAGGTGATCC CGCTGGACCG AGAGAACCGG GAGCTCAAGG CCTTCAACAT CCGGCTGGAG 
GAGCTGCAGG TCATCGATGT CCAGTTCCTG TACGGCTGCC AGGCCCCCAC CGTCTGCTTC 
ATATACCAGG ACCCGCAGGG CCGACACGTG AAGACATACG AGGTTTCCCT GCGGGAGAAG 
GAGTTCAATA AAGGGCCCTG GAAGCAGGAG AACGTGGAGG CCGAGGCATC GATGGTCATT 
CCAGTCCCGG AGCCGTTCGG CGGAGCCATC ATCATCGGGC AGGAGTCCAT CACCTACCAC 
AATGGAGATA AATACCTGGC CGTCGCTCCG CCCATTATCA AGCAAAGCAC CATCGTGTGC 
CACAACCGTG TGGACCCGAA CGGCTCGCGG TACCTGCTGG GCGATATGGA GGGCCGGCTG 
TTCATGCTGC TGCTAGAGAA GGAGGAGCTG ATGGACGGAG CCGTGGTGCT GAAGGACCTG 
CATGTGGAGC TGCTGGGAGA GACGTCCATC GCCGAGTGTT TGACCTATCT GGATAACGGG 
GTGGTGTTTG TGGGATCCAG ACTGGGAGAC TCTCAGCTGG TGAAGCTGAA CGTGGACAGT 
AATGACCAGG GCTCGTATGT GGGGGTGATG GAGACCTTCA CTAACCTGGG CCCAATAGTG 
GACATGTGTG TGGTGGACCT GGAGCGGCAA GGGCAAGGCC AGTTGGTGAC GTGCTCCGGA 
GCGTTTAAGG AAGGCTCTCT CAGGATCATC CGGAACGGTA TTGGGATCCA CGAGCACGCC 
AGCATCGACC TGCCGGGAAT CAAAGGTCTG TGGCCTCTTC GCTCAGAGTC CAGCAGAGAC 
ACTGATGATA TGCTGGTTTT GTCTTTCGTT GGTCAGACCA GGGTGCTGAT GCTGAGTGGA 
GAGGAGGTGG AGGAGACGGA GCTGCAGGGG TTTGTGGATA ACCAGCAGAC CTTCTTCTGT 
GGGAATGTGG CGCATCAGCA GCTCATACAG ATCACCTCGG TGTCGGTGCG GCTGGTCACT 
CAGGACAGTA AGGCTCTAGT CAGCGAGTGG AAGGAGCCGC AGGGCAGGAA CATCAGCGTG 
GCGTCCTGCA ACAACACACA GGTGGTGCTG GCGGTGGGCA GAGTGCTGTA CTACCTGCAG 
ATCCTGAGCG GAGAACTCAA GCAGATCAGC TCTACAGAGA TGGAGCATGA GGTGGCGTGT 
CTGGACATCA CTCCTCTGGG CGAGCGCACT GCTGACTCCT GCATCTGTGC TGTGGGGCTC 
TGGACCGACA TCTCCGCCCG CCTGCTCAAG CTGCCCTGCT TCACCCCTCT GCACAAGGAG 
ATGCTGGGCG GAGAGATCAT CCCTCGCTCC ATCCTCATGA CCACCTTCGA GGGCAGCCAC 
TACCTGCTGT GTGCGCTGGG AGACGGAGCG CTCTTCTACT TCGGCCTGGA TATTCAGACA 
GGTGTTCTGA GCGAGCGTAA GAAGGTGACT CTCGGCACTC AGCCCACCGT CCTGCGCACG 
TTCCGCTCGC TTTCCACCTC CAACGTGTTC GCCTGCTCCG ACCGGCCCAC CGTCATCTAC 
TCCAGCAACC ACAAGCTGGT GTTCTCCAAC GTCAACCTGA AGGAGGTCAA CTACATGTGC 
CCGCTCAACT CTGAGGGCTA CCCGGACAGT CTGGCTCTGG CCAATAACAG CACTCTCACC 
ATCGGCACCA TTGACGAGAT CCAGAAGCTC CACATCCGGA CTGTGCCGCT CTACGAGTCC 
CCCAAGCGGA TCTGTTATCA GGAGGTGTCT CAGTGTTTTG GGGTTTTGTC CAGTAGAGTT 
GAAATGCAGG ACGCCAGTGG AACCACAGCA GCCGTACGGC CCAGCGCCAG CACACAGGCT 
CTGTCCAGCA GTGTGAGCTC CAGTAAGCTG CTCCCCAGCA GCACGTCGCC CCATGAGACG 
TCATTCGGGG AGGAGGTGGA GGTGCACAGC TTACTGGTGG TGGACCAGCA CACGTTTGAG 
GTGCTGCACG CTCATCAGTT CCTGCAGAAC GAGTACGCCC TCAGCATGGT GTCCTGCAAA 
CTGGGTCGAG ATCCTGCTGT TTACTTCATC GTCGGCACGG CGATGGTTTA CCCAGAGGAG 
GCGGAGCCAA AGCAGGGCCG CATCATCGTC TTCCACTATA CTGACGGCAA ACTGCAGACG 
GTGGCGGAGA AGGAGGTGAA GGGTGCCGTT TACTCCATGG TGGAGTTCAA TGGTAAACTG 
CTGGCCAGCA TCAACAGCAC GGTGCGTCTG TACGAGTGGA CCGCAGAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Loss of DDB1 Leads to Transcriptional p53 Pathway Activation in Proliferating Cells, Cell Cycle Deregulation, and Apoptosis in Zebrafish Embryos.
PloS one10(7)e0134299(2015)
Hu Z,Holzschuh J,Driever W

Proteome dynamics in neutrophils of adult zebrafish upon chemically-induced inflammation.
Fish & shellfish immunology40(1)217-24(2014 Sep)
Singh SK,Aravamudhan S,Armant O,Kr?ger M,Grabher C

Selection of reliable biomarkers from PCR array analyses using relative distance computational model: methodology and proof-of-concept study.
PloS one8(12)e83954(2013)
Liu C,Xu H,Lam SH,Gong Z

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ddb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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