EFTUD2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFTUD2
Entrez Gene ID	393480
Full Name	elongation factor Tu GTP binding domain containing 2
Synonyms	wu:fi20f05,zgc:66214
General protein information	Preferred Names elongation factor Tu GTP binding domain containing 2 Names 116 kDa U5 small nuclear ribonucleoprotein component
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200508.2	NP_956802.2	116 kDa U5 small nuclear ribonucleoprotein component
XM_017353590.1	XP_017209079.1	116 kDa U5 small nuclear ribonucleoprotein component isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EFTUD2	XP_005624439.1
Saccharomyces cerevisiae (baker's yeast)	SNU114	NP_012748.1
Eremothecium gossypii	AGOS_AEL124W	NP_984737.1
Schizosaccharomyces pombe (fission yeast)	cwf10	NP_596689.2
Xenopus tropicalis (tropical clawed frog)	eftud2	NP_001025537.1
Homo sapiens (human)	EFTUD2	NP_001245282.1
Pan troglodytes (chimpanzee)	EFTUD2	XP_001145980.1
Oryza sativa (rice)	Os06g0608300	NP_001058038.1
Rattus norvegicus (Norway rat)	Eftud2	XP_213492.3
Arabidopsis thaliana (thale cress)	MEE5	NP_172112.1
Arabidopsis thaliana (thale cress)	AT5G25230	NP_197905.1
Mus musculus (house mouse)	Eftud2	NP_001103465.1
Danio rerio (zebrafish)	eftud2	NP_956802.2
Drosophila melanogaster (fruit fly)	CG4849	NP_651605.1
	EFTUD2	XP_001114964.1
Bos taurus (cattle)	EFTUD2	NP_001076865.1
Gallus gallus (chicken)	EFTUD2	NP_001026672.1
Caenorhabditis elegans (roundworm)	eftu-2	NP_498308.1
Kluyveromyces lactis	KLLA0A06688g	XP_451298.1
Magnaporthe oryzae (rice blast fungus)	MGG_13500	XP_003716278.1
Neurospora crassa	NCU02572	XP_965703.2

Gene Ontology
Bibliography

Related articles in PubMed

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Lei L, Yan SY, Yang R, Chen JY, Li Y, Bu Y, Chang N, Zhou Q, Zhu X, Li CY, Xiong JW
Nucleic acids research45(6)3422-3436(2017 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

EFTUD2 deficiency in vertebrates: Identification of a novel human mutation and generation of a zebrafish model.
Deml B, Reis LM, Muheisen S, Bick D, Semina EV
Birth defects research. Part A, Clinical and molecular teratology103(7)630-40(2015 Jul)

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Liu Y, Chen X, Qin B, Zhao K, Zhao Q, Staley JP, Zhao C
Journal of ophthalmology2015816329(2015)

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Chen X, Liu Y, Sheng X, Tam PO, Zhao K, Chen X, Rong W, Liu Y, Liu X, Pan X, Chen LJ, Zhao Q, Vollrath D, Pang CP, Zhao C
Human molecular genetics23(11)2926-39(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study has established that eftud2 functions in RNA splicing during neural development
Title: Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.

The following EFTUD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFTUD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02057	NM_200508.2 Latest version!	Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa58028	XM_017353590.1 Latest version!	Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFTUD2 ( NM_200508.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_956802.2 Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Price & Availability↑

CloneID	ODa02057
Clone ID Related Accession (Same CDS sequence)	NM_200508.2
Accession Version	NM_200508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	116 kDa U5 small nuclear ribonucleoprotein component
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154433.1. On or before Mar 12, 2008 this sequence version replaced XM_678499.2, NM_200508.1. ##Evidence-Data-START## Transcript exon combination :: BC154433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGACTG ATCTTTATGA CGAGTTTGGA AATTATATCG GACCAGAGCT GGACTCTGAT 
GAGGATGAAG AACTAGATGC AGAGGACCGG GATGCTGATG AGGCGGATGA GGAGGGTGAC 
GATGATGACC AGGCTGAGGC TGATGAGGAT GGAGGAGGTG GTGGTATGGA GGTGGTTCTG 
CATGAGGATA AAAAGTATTA TCCCACTGCT GAAGAAGTGT ATGGTCCTGA GGTGGAAACT 
ATTGTTCAAG AAGAAGATAC ACAGCCTCTG ACAGAGCCCA TTATAAAACC TGTGAGGATG 
AAGCAGTTTA CTCTGATGGA GCAGGAGCTT CCAGCGACCG TCTATGACAT GGAATTCCTT 
GCTGATCTTA TGGACAGCTC AGAACTGATC AGAAATGTCA CACTGTGTGG ACACTTACAC 
CACGGCAAGA CATGTTTTGT AGACTGCCTC ATTGAACAGA CTCATCCTGA AATCCGTAAG 
AGAGATGATG AAGATTTACG ATACACAGAT ATCCTTTTCA CTGAGCAAGA GAGAGGGGTT 
GGCATCAAGA GTACTCCTGT AACCATGGTG CTGCCGGACT CACGGGGCAA ATCCTACCTC 
TTCAACATCA TGGACACGCC AGGTCATGTG AATTTCTCTG ATGAAGTGAC ATCTGCAGTT 
AGACTATCTG ACGGGATTGT GCTTTTTATT GATGCTGCTG AGGGGGTAAT GCTGAACACT 
GAGCGACTGA TCAAGCATGC AGTGCAGGAG AGATTGGCCA TCACTATCTG TATTAATAAA 
ATAGATCGTC TGATTGTGGA GCTCAAACTG CCCCCTACTG ATGCCTACTA CAAATTGCGC 
CACATTGTGG ATGAGGTCAA CGGCTTGCTG AGCACATATT CTACAGATGA GTCTCTGATT 
GTGTCGCCTC TGTTGGGGAA CGTCTGTTTC GCTTCCTCGC AGTACTGCAT TTGCTTTACT 
CTCGGCTCCT TCGCCAAAAT CTATTCAGAC ACCTACGGTG ACATTAGCTA CATGGAGTTC 
GCAAAAAGAC TATGGGGAGA CATTTACTTC AACCCCAAAA CGAGAAAGTT CACAAAGAAA 
GCCCCCAACA GCAACTCCCA GCGCAGCTTT GTTGAGTTTG TGCTGGAGCC GCTGTACAAG 
ATCCTTTCAC AGGTGGTGGG TGATGTGGAT ACGTCTCTGC CGCGAGTCCT TGACGAACTT 
GGCATTCATC TGACCAAAGA GGAGTTAAAA CTGAACATCA AACCTCTGCT GCGCCTCGTC 
TGTAACCGCT TTTTTGGAGA GTTTACAGGC TTAGTGGACA TGTGTGTGCA ACACATACCA 
TCTCCTCAGG GGGGCGCCAG AGCAAAAATA GAGCATACCT ACACGGGTGG ACTGGACTCA 
GACCTCGGAG AGACCATGTC TGAGTGTGAC CCTGATGGTC CACTGATGTG CCACACCACT 
AAGATGTACA GCACAGATGA CGGCGTTCAG TTCCACGCGT TTGGCCGGGT CCTAAGTGGA 
ACCCTGCAGG CGGGTCAGCC CGTGAAGGTT CTGGGAGAAA ACTACAGTCT GGAGGACGAG 
GAGGATTCTC AGATCTGCAC TGTGGGACGC CTGTGGATTT CTGTCGCCAG GTATCAGATT 
GAAGTGAATC GAGTCCCTGC TGGTAACTGG GTGTTAATTG AGGGCTGTGA CCAGCCGATT 
GTTAAAACAG CCACAATCAC AGAGCCCCGT GGAAATGAAG AGGCACAGAT CTTCAGGCCA 
CTCAAGTTTA ACACCGCATC AGTTATAAAA ATTGCTGTGG AGCCTGTCAA TCCTTCAGAA 
TTGCCCAAGA TGTTGGATGG ACTCCGAAAA GTTAACAAAA GTTATCCTTC ACTCACAACA 
AAGGTGGAGG AGTCAGGTGA ACACGTGATT CTTGGCATAG GTGAGCTGTA TCTGGACTGT 
GTAATGCACG ACCTGCGGAA AATGTACTCT GAAATTGATA TCAAGGTAGC TGATCCTGTG 
GTGACCTTTT GTGAGACTGT CGTGGAAACT TCCTCTTTGA AGTGTTTTGC TGAAACGCCC 
AATAAAAAGA ATAAAATCAC CATGATTGCT GAGCCATTGG AGAAGGGTCT TGCTGAAGAC 
ATAGAGAACG AGGTGGTGCA GATCACATGG AACAGGAAAA AGTTGGGAGA GTTTTTCCAG 
ACCAAGTATG ATTGGGATTT GCTGGCAGCT CGTTCCATTT GGGCTTTTGG ACCAGACACA 
ACTGGACCCA ACATCTTAGT AGACGACACT TTGCCATCTG AGGTGGATAA AGCTCTCCTC 
GGCTCAGTGA AAGACAGTAT TGTACAGGGT TTCCAGTGGG GAACACGCGA GGGTCCTCTG 
TGTGACGAAC CAATCCGCAA TGTGAAGTTC AAGATCCTGG ATGCTGTGAT TGCTCAGGAG 
CCGCTGCACA GAGGAGGAGG ACAAGTCATC CCTACAGCTC GCAGAGTCGT CTACTCCGCT 
TTCCTTATGG CCACGCCCAG GCTAATGGAG CCCTATTACT TTGTGGAGGT TCAAGCACCT 
GCTGACTGTG TTTCTGCAGT TTACACAGTG TTGGCACGCA GAAGAGGTCA TGTGACGCAG 
GACGCTCCAA TCCCTGGCTC TCCTCTCTAC ACCATCAAGG CTTTCATCCC TGCTATTGAC 
TCGTTCGGCT TTGAAACGGA TCTACGCACA CACACACAGG GCCAGGCCTT CGCGCTCAGT 
GTGTTTCACC ACTGGCAGAT TGTCCCCGGT GATCCTCTGG ATAAGAGCAT TGTGATTCGT 
CCTCTAGAGC CCCAACCGGC CCCTCATCTG GCCAGAGAGT TTATGATCAA GACCCGCAGG 
CGCAAAGGCT TGAGCGAGGA TGTCAGTATC AGCAAGTTCT TCGATGACCC CATGTTGTTG 
GAGCTCGCCA AACAGGACGT GATGCTCAAC TACCCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956802.2
CDS	142..3063
Translation	METDLYDEFGNYIGPELDSDEDEELDAEDRDADEADEEGDDDDQAEADEDGGGGGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVRMKQFTLMEQELPATVYDMEFLADLMDSSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRDDEDLRYTDILFTEQERGVGIKSTPVTMVLPDSRGKSYLFNIMDTPGHVNFSDEVTSAVRLSDGIVLFIDAAEGVMLNTERLIKHAVQERLAITICINKIDRLIVELKLPPTDAYYKLRHIVDEVNGLLSTYSTDESLIVSPLLGNVCFASSQYCICFTLGSFAKIYSDTYGDISYMEFAKRLWGDIYFNPKTRKFTKKAPNSNSQRSFVEFVLEPLYKILSQVVGDVDTSLPRVLDELGIHLTKEELKLNIKPLLRLVCNRFFGEFTGLVDMCVQHIPSPQGGARAKIEHTYTGGLDSDLGETMSECDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTLQAGQPVKVLGENYSLEDEEDSQICTVGRLWISVARYQIEVNRVPAGNWVLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGIGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFALSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVMLNYPM

Target ORF information:

RefSeq Version	NM_200508.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200508.2

ORF Insert Sequence:

ATGGAGACTG ATCTTTATGA CGAGTTTGGA AATTATATCG GACCAGAGCT GGACTCTGAT 
GAGGATGAAG AACTAGATGC AGAGGACCGG GATGCTGATG AGGCGGATGA GGAGGGTGAC 
GATGATGACC AGGCTGAGGC TGATGAGGAT GGAGGAGGTG GTGGTATGGA GGTGGTTCTG 
CATGAGGATA AAAAGTATTA TCCCACTGCT GAAGAAGTGT ATGGTCCTGA GGTGGAAACT 
ATTGTTCAAG AAGAAGATAC ACAGCCTCTG ACAGAGCCCA TTATAAAACC TGTGAGGATG 
AAGCAGTTTA CTCTGATGGA GCAGGAGCTT CCAGCGACCG TCTATGACAT GGAATTCCTT 
GCTGATCTTA TGGACAGCTC AGAACTGATC AGAAATGTCA CACTGTGTGG ACACTTACAC 
CACGGCAAGA CATGTTTTGT AGACTGCCTC ATTGAACAGA CTCATCCTGA AATCCGTAAG 
AGAGATGATG AAGATTTACG ATACACAGAT ATCCTTTTCA CTGAGCAAGA GAGAGGGGTT 
GGCATCAAGA GTACTCCTGT AACCATGGTG CTGCCGGACT CACGGGGCAA ATCCTACCTC 
TTCAACATCA TGGACACGCC AGGTCATGTG AATTTCTCTG ATGAAGTGAC ATCTGCAGTT 
AGACTATCTG ACGGGATTGT GCTTTTTATT GATGCTGCTG AGGGGGTAAT GCTGAACACT 
GAGCGACTGA TCAAGCATGC AGTGCAGGAG AGATTGGCCA TCACTATCTG TATTAATAAA 
ATAGATCGTC TGATTGTGGA GCTCAAACTG CCCCCTACTG ATGCCTACTA CAAATTGCGC 
CACATTGTGG ATGAGGTCAA CGGCTTGCTG AGCACATATT CTACAGATGA GTCTCTGATT 
GTGTCGCCTC TGTTGGGGAA CGTCTGTTTC GCTTCCTCGC AGTACTGCAT TTGCTTTACT 
CTCGGCTCCT TCGCCAAAAT CTATTCAGAC ACCTACGGTG ACATTAGCTA CATGGAGTTC 
GCAAAAAGAC TATGGGGAGA CATTTACTTC AACCCCAAAA CGAGAAAGTT CACAAAGAAA 
GCCCCCAACA GCAACTCCCA GCGCAGCTTT GTTGAGTTTG TGCTGGAGCC GCTGTACAAG 
ATCCTTTCAC AGGTGGTGGG TGATGTGGAT ACGTCTCTGC CGCGAGTCCT TGACGAACTT 
GGCATTCATC TGACCAAAGA GGAGTTAAAA CTGAACATCA AACCTCTGCT GCGCCTCGTC 
TGTAACCGCT TTTTTGGAGA GTTTACAGGC TTAGTGGACA TGTGTGTGCA ACACATACCA 
TCTCCTCAGG GGGGCGCCAG AGCAAAAATA GAGCATACCT ACACGGGTGG ACTGGACTCA 
GACCTCGGAG AGACCATGTC TGAGTGTGAC CCTGATGGTC CACTGATGTG CCACACCACT 
AAGATGTACA GCACAGATGA CGGCGTTCAG TTCCACGCGT TTGGCCGGGT CCTAAGTGGA 
ACCCTGCAGG CGGGTCAGCC CGTGAAGGTT CTGGGAGAAA ACTACAGTCT GGAGGACGAG 
GAGGATTCTC AGATCTGCAC TGTGGGACGC CTGTGGATTT CTGTCGCCAG GTATCAGATT 
GAAGTGAATC GAGTCCCTGC TGGTAACTGG GTGTTAATTG AGGGCTGTGA CCAGCCGATT 
GTTAAAACAG CCACAATCAC AGAGCCCCGT GGAAATGAAG AGGCACAGAT CTTCAGGCCA 
CTCAAGTTTA ACACCGCATC AGTTATAAAA ATTGCTGTGG AGCCTGTCAA TCCTTCAGAA 
TTGCCCAAGA TGTTGGATGG ACTCCGAAAA GTTAACAAAA GTTATCCTTC ACTCACAACA 
AAGGTGGAGG AGTCAGGTGA ACACGTGATT CTTGGCATAG GTGAGCTGTA TCTGGACTGT 
GTAATGCACG ACCTGCGGAA AATGTACTCT GAAATTGATA TCAAGGTAGC TGATCCTGTG 
GTGACCTTTT GTGAGACTGT CGTGGAAACT TCCTCTTTGA AGTGTTTTGC TGAAACGCCC 
AATAAAAAGA ATAAAATCAC CATGATTGCT GAGCCATTGG AGAAGGGTCT TGCTGAAGAC 
ATAGAGAACG AGGTGGTGCA GATCACATGG AACAGGAAAA AGTTGGGAGA GTTTTTCCAG 
ACCAAGTATG ATTGGGATTT GCTGGCAGCT CGTTCCATTT GGGCTTTTGG ACCAGACACA 
ACTGGACCCA ACATCTTAGT AGACGACACT TTGCCATCTG AGGTGGATAA AGCTCTCCTC 
GGCTCAGTGA AAGACAGTAT TGTACAGGGT TTCCAGTGGG GAACACGCGA GGGTCCTCTG 
TGTGACGAAC CAATCCGCAA TGTGAAGTTC AAGATCCTGG ATGCTGTGAT TGCTCAGGAG 
CCGCTGCACA GAGGAGGAGG ACAAGTCATC CCTACAGCTC GCAGAGTCGT CTACTCCGCT 
TTCCTTATGG CCACGCCCAG GCTAATGGAG CCCTATTACT TTGTGGAGGT TCAAGCACCT 
GCTGACTGTG TTTCTGCAGT TTACACAGTG TTGGCACGCA GAAGAGGTCA TGTGACGCAG 
GACGCTCCAA TCCCTGGCTC TCCTCTCTAC ACCATCAAGG CTTTCATCCC TGCTATTGAC 
TCGTTCGGCT TTGAAACGGA TCTACGCACA CACACACAGG GCCAGGCCTT CGCGCTCAGT 
GTGTTTCACC ACTGGCAGAT TGTCCCCGGT GATCCTCTGG ATAAGAGCAT TGTGATTCGT 
CCTCTAGAGC CCCAACCGGC CCCTCATCTG GCCAGAGAGT TTATGATCAA GACCCGCAGG 
CGCAAAGGCT TGAGCGAGGA TGTCAGTATC AGCAAGTTCT TCGATGACCC CATGTTGTTG 
GAGCTCGCCA AACAGGACGT GATGCTCAAC TACCCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFTUD2 ( XM_017353590.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209079.1 Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58028
Clone ID Related Accession (Same CDS sequence)	XM_017353590.1
Accession Version	XM_017353590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	116 kDa U5 small nuclear ribonucleoprotein component isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG ATCTTTATGA CGAGTTTGGA AATTATATCG GACCAGAGCT GGACTCTGAT 
GAGGATGAAG AGCTAGATGC AGAGGACCGG GATGCTGATG AGGCGGATGA GGAGGATGAC 
GATGATGACC AGGCTGAGGC TGATGAGGAT GGAGGAGGTG GTGGTATGGA GGTGGTTCTG 
CATGAGGATA AAAAGTATTA TCCCACTGCT GAAGAAGTGT ATGGTCCTGA GGTGGAAACT 
ATTGTTCAAG AAGAAGATAC ACAGCCGCTG ACAGAGCCCA TTATAAAACC TGTGAGGATG 
AAGCAGTTTA CTCTGATGGA GCAGGAGCTT CCAGCGACCG TCTATGACAT GGAATTCCTT 
GCTGATCTTA TGGACAGCTC AGAACTGATC AGAAATGTCA CACTGTGTGG ACACTTACAC 
CACGGCAAGA CATGTTTTGT AGACTGCCTC ATTGAACAGA CTCATCCTGA AATCCGTAAG 
AGAGATGACG AAGATTTACG ATACACAGAT ATCCTTTTCA CTGAGCAAGA GAGAGGGGTT 
GGCATCAAGA GTACTCCTGT AACCATGGTG CTGCCGGACT CACGGGGCAA ATCCTACCTC 
TTCAACATCA TGGACACGCC AGGTCATGTG AATTTCTCTG ATGAAGTGAC ATCTGCAGTT 
AGACTATCTG ACGGGATTGT GCTTTTTATT GATGCTGCTG AGGGGGTAAT GCTGAACACT 
GAGCGACTGA TCAAGCATGC AGTGCAGGAG AGATTGGCCA TCACTATCTG TATTAATAAA 
ATAGATCGTC TGATTGTGGA GCTCAAACTG CCCCCTACTG ATGCCTACTA CAAATTGCGC 
CACATTGTGG ATGAGGTCAA CGGCTTGCTG AGCACATATT CTACAGATGA GTCTCTGATT 
GTGTCGCCTC TGTTGGGGAA CGTCTGTTTC GCTTCCTCGC AGTACTGCAT TTGCTTTACT 
CTCGGCTCCT TCGCCAAAAT CTATTCAGAC ACCTACGGTG ACATTAGCTA CATGGAGTTC 
GCAAAAAGAC TATGGGGAGA CATTTACTTC AACCCCAAAA CGAGAAAGTT CACAAAGAAA 
GCCCCCAACA GCAACTCCCA GCGCAGCTTT GTTGAGTTTG TGCTGGAGCC GCTGTACAAG 
ATCCTTTCAC AGGTGGTGGG TGATGTGGAT ACGTCTCTGC CGCGAGTCCT TGACGAACTT 
GGCATTCATC TGACCAAAGA GGAGTTAAAA CTCAACATCA AACCTCTGCT GCGCCTCGTC 
TGTAACCGCT TTTTTGGAGA GTTTACAGGC TTAGTGGACA TGTGTGTGCA ACACATACCA 
TCTCCTCAGG GGGGCGCCAG AGCAAAAATA GAACATACCT ACACGGGTGG ACTGGACTCA 
GACCTCGGAG AGACCATGTC TGAGTGTGAC CCTGATGGTC CACTGATGTG CCACACCACT 
AAGATGTACA GCACAGATGA CGGCGTTCAG TTCCACGCGT TTGGTCGGGT CCTAAGTGGA 
ACCCTGCAGG CAGGTCAGCC CGTGAAGGTT CTGGGAGAAA ACTACAGTCT GGAGGACGAG 
GAGGATTCTC AGATCTGCAC TGTGGGACGC CTGTGGATTT CTGTCGCCAG GTATCAGATT 
GAAGTGAATC GAGTCCCTGC TGGTAACTGG GTGTTAATTG AGGGCTGTGA CCAGCCGATT 
GTTAAAACAG CCACAATCAC AGAGCCCCGT GGAAATGAAG AGGCACAGAT CTTCAGGCCA 
CTCAAGTTTA ACACCGCATC AGTTATAAAA ATTGCTGTGG AGCCTGTCAA TCCTTCAGAA 
TTGCCCAAGA TGTTGGATGG ACTCCGAAAA GTTAACAAAA GTTATCCTTC ACTCACAACA 
AAGGTGGAGG AGTCAGGTGA ACACGTGATT CTTGGCACAG GTGAGCTGTA TCTGGACTGT 
GTAATGCACG ACCTGCGGAA AATGTACTCT GAAATTGATA TCAAGGTAGC TGATCCTGTG 
GTGACCTTTT GTGAGACTGT CGTGGAAACT TCTTCTTTGA AGTGTTTTGC TGAAACGCCC 
AATAAAAAGA ATAAAATCAC CATGATTGCT GAGCCATTGG AGAAGGGTCT TGCTGAAGAC 
ATTGAGAACG AGGTGGTGCA GATCACATGG AACAGGAAAA AGTTGGGAGA GTTTTTCCAG 
ACCAAGTATG ATTGGGATTT GCTGGCAGCT CGTTCCATTT GGGCTTTTGG ACCAGACACA 
ACTGGACCCA ACATCTTAGT AGACGACACT TTGCCATCTG AGGTGGATAA AGCTCTCCTT 
GGCTCAGTGA AAGACAGTAT TGTACAGGGT TTCCAGTGGG GAACACGCGA GGGTCCTCTG 
TGTGACGAAC CAATCCGCAA TGTGAAGTTC AAGATCCTGG ATGCCGTGAT TGCTCAGGAG 
CCGCTGCACA GAGGAGGAGG ACAAGTCATC CCTACAGCTC GCAGAGTTGT CTACTCCGCT 
TTCCTCATGG CCACACCCAG GCTAATGGAG CCCTATTACT TTGTGGAGGT TCAAGCACCT 
GCTGACTGTG TTTCTGCAGT TTACACAGTG TTGGCACGCA GAAGAGGTCA TGTGACGCAG 
GACGCTCCAA TCCCTGGCTC TCCTCTCTAC ACCATCAAGG CTTTCATCCC TGCTATTGAC 
TCATTCGGCT TTGAAACGGA TCTACGCACA CACACACAGG GCCAGGCCTT CGCGCTCAGT 
GTGTTTCACC ACTGGCAGAT TGTCCCCGGT GATCCTCTGG ATAAGAGCAT TGTGATTCGT 
CCTTTAGAGC CCCAACCGGC CCCTCATCTG GCCAGAGAGT TTATGATCAA GACCCGCAGG 
CGCAAAGGCT TGAGCGAGGA TGTCAGTATC AGCAAGTTCT TCGATGACCC CATGTTGTTG 
GAGCTCGCCA AACAGGACGT GATGCTCAAC TACCCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209079.1
CDS	32..2953
Translation	METDLYDEFGNYIGPELDSDEDEELDAEDRDADEADEEDDDDDQAEADEDGGGGGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVRMKQFTLMEQELPATVYDMEFLADLMDSSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRDDEDLRYTDILFTEQERGVGIKSTPVTMVLPDSRGKSYLFNIMDTPGHVNFSDEVTSAVRLSDGIVLFIDAAEGVMLNTERLIKHAVQERLAITICINKIDRLIVELKLPPTDAYYKLRHIVDEVNGLLSTYSTDESLIVSPLLGNVCFASSQYCICFTLGSFAKIYSDTYGDISYMEFAKRLWGDIYFNPKTRKFTKKAPNSNSQRSFVEFVLEPLYKILSQVVGDVDTSLPRVLDELGIHLTKEELKLNIKPLLRLVCNRFFGEFTGLVDMCVQHIPSPQGGARAKIEHTYTGGLDSDLGETMSECDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTLQAGQPVKVLGENYSLEDEEDSQICTVGRLWISVARYQIEVNRVPAGNWVLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFALSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVMLNYPM

Target ORF information:

RefSeq Version	XM_017353590.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio elongation factor Tu GTP binding domain containing 2 (eftud2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353590.1

ORF Insert Sequence:

ATGGAGACTG ATCTTTATGA CGAGTTTGGA AATTATATCG GACCAGAGCT GGACTCTGAT 
GAGGATGAAG AGCTAGATGC AGAGGACCGG GATGCTGATG AGGCGGATGA GGAGGATGAC 
GATGATGACC AGGCTGAGGC TGATGAGGAT GGAGGAGGTG GTGGTATGGA GGTGGTTCTG 
CATGAGGATA AAAAGTATTA TCCCACTGCT GAAGAAGTGT ATGGTCCTGA GGTGGAAACT 
ATTGTTCAAG AAGAAGATAC ACAGCCGCTG ACAGAGCCCA TTATAAAACC TGTGAGGATG 
AAGCAGTTTA CTCTGATGGA GCAGGAGCTT CCAGCGACCG TCTATGACAT GGAATTCCTT 
GCTGATCTTA TGGACAGCTC AGAACTGATC AGAAATGTCA CACTGTGTGG ACACTTACAC 
CACGGCAAGA CATGTTTTGT AGACTGCCTC ATTGAACAGA CTCATCCTGA AATCCGTAAG 
AGAGATGACG AAGATTTACG ATACACAGAT ATCCTTTTCA CTGAGCAAGA GAGAGGGGTT 
GGCATCAAGA GTACTCCTGT AACCATGGTG CTGCCGGACT CACGGGGCAA ATCCTACCTC 
TTCAACATCA TGGACACGCC AGGTCATGTG AATTTCTCTG ATGAAGTGAC ATCTGCAGTT 
AGACTATCTG ACGGGATTGT GCTTTTTATT GATGCTGCTG AGGGGGTAAT GCTGAACACT 
GAGCGACTGA TCAAGCATGC AGTGCAGGAG AGATTGGCCA TCACTATCTG TATTAATAAA 
ATAGATCGTC TGATTGTGGA GCTCAAACTG CCCCCTACTG ATGCCTACTA CAAATTGCGC 
CACATTGTGG ATGAGGTCAA CGGCTTGCTG AGCACATATT CTACAGATGA GTCTCTGATT 
GTGTCGCCTC TGTTGGGGAA CGTCTGTTTC GCTTCCTCGC AGTACTGCAT TTGCTTTACT 
CTCGGCTCCT TCGCCAAAAT CTATTCAGAC ACCTACGGTG ACATTAGCTA CATGGAGTTC 
GCAAAAAGAC TATGGGGAGA CATTTACTTC AACCCCAAAA CGAGAAAGTT CACAAAGAAA 
GCCCCCAACA GCAACTCCCA GCGCAGCTTT GTTGAGTTTG TGCTGGAGCC GCTGTACAAG 
ATCCTTTCAC AGGTGGTGGG TGATGTGGAT ACGTCTCTGC CGCGAGTCCT TGACGAACTT 
GGCATTCATC TGACCAAAGA GGAGTTAAAA CTCAACATCA AACCTCTGCT GCGCCTCGTC 
TGTAACCGCT TTTTTGGAGA GTTTACAGGC TTAGTGGACA TGTGTGTGCA ACACATACCA 
TCTCCTCAGG GGGGCGCCAG AGCAAAAATA GAACATACCT ACACGGGTGG ACTGGACTCA 
GACCTCGGAG AGACCATGTC TGAGTGTGAC CCTGATGGTC CACTGATGTG CCACACCACT 
AAGATGTACA GCACAGATGA CGGCGTTCAG TTCCACGCGT TTGGTCGGGT CCTAAGTGGA 
ACCCTGCAGG CAGGTCAGCC CGTGAAGGTT CTGGGAGAAA ACTACAGTCT GGAGGACGAG 
GAGGATTCTC AGATCTGCAC TGTGGGACGC CTGTGGATTT CTGTCGCCAG GTATCAGATT 
GAAGTGAATC GAGTCCCTGC TGGTAACTGG GTGTTAATTG AGGGCTGTGA CCAGCCGATT 
GTTAAAACAG CCACAATCAC AGAGCCCCGT GGAAATGAAG AGGCACAGAT CTTCAGGCCA 
CTCAAGTTTA ACACCGCATC AGTTATAAAA ATTGCTGTGG AGCCTGTCAA TCCTTCAGAA 
TTGCCCAAGA TGTTGGATGG ACTCCGAAAA GTTAACAAAA GTTATCCTTC ACTCACAACA 
AAGGTGGAGG AGTCAGGTGA ACACGTGATT CTTGGCACAG GTGAGCTGTA TCTGGACTGT 
GTAATGCACG ACCTGCGGAA AATGTACTCT GAAATTGATA TCAAGGTAGC TGATCCTGTG 
GTGACCTTTT GTGAGACTGT CGTGGAAACT TCTTCTTTGA AGTGTTTTGC TGAAACGCCC 
AATAAAAAGA ATAAAATCAC CATGATTGCT GAGCCATTGG AGAAGGGTCT TGCTGAAGAC 
ATTGAGAACG AGGTGGTGCA GATCACATGG AACAGGAAAA AGTTGGGAGA GTTTTTCCAG 
ACCAAGTATG ATTGGGATTT GCTGGCAGCT CGTTCCATTT GGGCTTTTGG ACCAGACACA 
ACTGGACCCA ACATCTTAGT AGACGACACT TTGCCATCTG AGGTGGATAA AGCTCTCCTT 
GGCTCAGTGA AAGACAGTAT TGTACAGGGT TTCCAGTGGG GAACACGCGA GGGTCCTCTG 
TGTGACGAAC CAATCCGCAA TGTGAAGTTC AAGATCCTGG ATGCCGTGAT TGCTCAGGAG 
CCGCTGCACA GAGGAGGAGG ACAAGTCATC CCTACAGCTC GCAGAGTTGT CTACTCCGCT 
TTCCTCATGG CCACACCCAG GCTAATGGAG CCCTATTACT TTGTGGAGGT TCAAGCACCT 
GCTGACTGTG TTTCTGCAGT TTACACAGTG TTGGCACGCA GAAGAGGTCA TGTGACGCAG 
GACGCTCCAA TCCCTGGCTC TCCTCTCTAC ACCATCAAGG CTTTCATCCC TGCTATTGAC 
TCATTCGGCT TTGAAACGGA TCTACGCACA CACACACAGG GCCAGGCCTT CGCGCTCAGT 
GTGTTTCACC ACTGGCAGAT TGTCCCCGGT GATCCTCTGG ATAAGAGCAT TGTGATTCGT 
CCTTTAGAGC CCCAACCGGC CCCTCATCTG GCCAGAGAGT TTATGATCAA GACCCGCAGG 
CGCAAAGGCT TGAGCGAGGA TGTCAGTATC AGCAAGTTCT TCGATGACCC CATGTTGTTG 
GAGCTCGCCA AACAGGACGT GATGCTCAAC TACCCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.
Nucleic acids research45(6)3422-3436(2017 Apr)
Lei L,Yan SY,Yang R,Chen JY,Li Y,Bu Y,Chang N,Zhou Q,Zhu X,Li CY,Xiong JW

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

EFTUD2 deficiency in vertebrates: Identification of a novel human mutation and generation of a zebrafish model.
Birth defects research. Part A, Clinical and molecular teratology103(7)630-40(2015 Jul)
Deml B,Reis LM,Muheisen S,Bick D,Semina EV

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Journal of ophthalmology2015816329(2015)
Liu Y,Chen X,Qin B,Zhao K,Zhao Q,Staley JP,Zhao C

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Human molecular genetics23(11)2926-39(2014 Jun)
Chen X,Liu Y,Sheng X,Tam PO,Zhao K,Chen X,Rong W,Liu Y,Liu X,Pan X,Chen LJ,Zhao Q,Vollrath D,Pang CP,Zhao C

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EFTUD2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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