clcnk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clcnk
Entrez Gene ID	393353
Full Name	chloride channel K
Synonyms	zgc:64141
General protein information	Preferred Names chloride channel K Names chloride channel protein ClC-Ka CIC-K
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469764.1	XP_021325439.1	chloride channel protein ClC-Ka isoform X1
NM_200382.1	NP_956676.1	chloride channel protein ClC-Ka

Pathways from BioSystems

Homologies

	CLCNKB	XP_001091784.2
Canis lupus familiaris (dog)	CLCNKA	XP_544547.2
Bos taurus (cattle)	CLCNKA	XP_005203433.1
Rattus norvegicus (Norway rat)	Clcnka	NP_445779.1
Homo sapiens (human)	CLCNKB	NP_000076.2
Mus musculus (house mouse)	Clcnka	NP_001139779.1
Pan troglodytes (chimpanzee)	CLCNKB	XP_003949353.1
Gallus gallus (chicken)	CLCNKB	XP_004947529.1
Danio rerio (zebrafish)	clcnk	NP_956676.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

Comparative transcriptomic analysis identifies evolutionarily conserved gene products in the vertebrate renal distal convoluted tubule.
Sugano Y, Cianciolo Cosentino C, Loffing-Cueni D, Neuhauss SCF, Loffing J
Pflugers Archiv : European journal of physiology469(7-8)859-867(2017 Aug)

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
Wang YF, Yan JJ, Tseng YC, Chen RD, Hwang PP
International journal of biological sciences11(10)1190-203(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clcnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clcnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34554	NM_200382.1 Latest version!	Danio rerio chloride channel K (clcnk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa151451	XM_021469764.1 Latest version!	Danio rerio chloride channel K (clcnk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clcnk ( NM_200382.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956676.1 Danio rerio chloride channel K (clcnk), mRNA.

Price & Availability↑

CloneID	ODa34554
Clone ID Related Accession (Same CDS sequence)	NM_200382.1
Accession Version	NM_200382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	chloride channel protein ClC-Ka
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053277.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06350654, SAMN06645146 [ECO:0000348] ##Evidence-Data-END##

ATGGACGATA TTTGCGTAAA TGTAAAGCAA CCAGAAAATG AAAATGATGA ACAGCGTGTA 
CAAGAAGACA AGAGAGAGGA AGCTACAGCG GAAGAAAAGC TAATAAGTGC TAATGAGCCT 
TCATGGAAAC CATGGCCACA ATCACGCTCA CAAATCAGAG AGTGTGTGCT GAATGTGAAG 
AAGCTCCTGG TGTCTCTCGC CAGCATGGAG TGGTACTGTT ATGCTCTGTT AGGGATCATC 
ACAGCTCTAA TGAGCTTCTT CATGGACATG ACTGTGGCGA AGCTGCTAAA CGCCCACCAG 
TGGCTGTACG GCTGTCTGAA GGGTCACCAC CTGCTGCAGT TCCTCTGCTG GACGCTTTAC 
CCAGCCTGCC TCTGTGCTCT GTCCACCAGC TTCGCCCACA GCGTCTGCCC ATACTCTGCA 
GGCTCTGGTG TTCCTGAAGC GAGGGCTATT CTCTTAGGAG TGGACATGCC AGATTACCTG 
TCTCTCTCCA ATCTGTTTGC TAAAATGTTT GGCCTGATAT GCACACTAGC CGCTGGCAGC 
ACTGTGTTTC TTGGCAAAGT GGGCCCATTT GTCCATTTAT CTATCATGGT TGGTGCATTT 
ATGAATCGTC TGCATGTCTC CTGTCGTGGC GGAAAGCAGA GAGCAGCTAA AGGAGAAATG 
TTGGTTGTGG CATCAGCGGT GGGAGTTGCG AGCTGTTTTG GGGCTCCAAT AAGCGGCGTT 
CTCTTCAGCA TAGAGGTGAT GGGGACTCAT TATTCAGTCA GCGATTATTG TCCCTGCTTC 
TTTGCAGCGG CGTGCGGCGC CATCACCTTC AGGCTGCTTT CTGTCTGTAT TGGAGATCAA 
GACACAATCC AAGCCCTGTT TAAGACCAGC TTTTCTACTG ATCTGCCTTT TCAGCCTTTT 
GAAATAATAA TTTTTGCTCT GCTTGGGCTG TTTTGCAGTA TTCTCAGCTG TATTTACTTA 
CTCTGTCATC GCTGGGCTCT GCGTTTTGTC AAAACAAACA AACCCATCAG CACATTTTTG 
GCAACAGAAA AGTCACTTTA TTCAGCCATA GTGGCATTTC TTCTAGCCTG CTTTACATTT 
TATCACTCGG CGGGACATTT CATAGCTTCT GAGCTCACAA TGAGGCAGCT GCTTACTTCG 
ATGCTAGATG GCACTTCCTG GTATAGCATT TCCCAGAATA TATCTGCACT GCCTGAGCCA 
CAAAACACAT TCTGGCAGGC CTGGAATCCA CCAGGCAAAA GTTACTTCCA AACGCTGACA 
TTTTTCATTA TCACCAAGGT GGGGCTGCTG GTCGTGGCTT GTACTTTACC TTTACCTGCT 
GGTTATTTCA TGCCAGTATT TGTTTATGGT GCAGCAGTGG GTCGTTTTAT CGGCGAAGGC 
TCTTTCTATC TGCTCTCAGG TGGAATCTCA TCAGGGAAGA GCCTGATAAA CCCAGGCGGC 
TACGCTTTAG CAGGAGCGGC TGCTTTCTCT GGGGCCGTGA CACACACACT TTCTCCTGCC 
CTGCTGGCGG TGGAGATGAC CGGTCAGTGT TCCTATGCTG TTCCCGCTCT GGTGGCGACG 
CTCGTGTCCA ATGCTGTGGC TCGGGCTAAA CACAGGCCGT CTTTTTATGA TGGCATATCG 
CTTATTAAAC GGCTCCCGCA CCTGCCGTCT CTCATCCGCG CATGTCCGAA GTTGGCCGAT 
GTTCAGATTA AGCAGTTTGT GATCCCAGCA GGAGCTGTTC TGGAAAGGAG CGAGAAGCTG 
ATAAGTGTTC AGCACATCCT AAAGGAAAGC ACAGACTCTG AACTACCTGT GGTGGAGTCA 
CGCGACTCCT CTGTTCTCCT GGGCACAATC AACAGATCGC AGCTGCGCGA GTGTCTGCTT 
GAGCATAGAA ATGAGGATTC ATGTAAACAG CTTGAAGAAG TGTGTTGTGT TCAGCCTGTG 
CGTCTTCATC TGACTCCTGA CAGCACAGTA AAGCAGGCTC ATTGCATCAT GAGCATCACG 
GGTGAGCAGC GTCTGTTTAT AACAGAGAGT GGGCGACTGT GTGGCGTCAT CACATGGAAA 
GAGATGAAAA AAATTATTGA AAAGATGGCA AAGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956676.1
CDS	40..2121
Translation	MDDICVNVKQPENENDEQRVQEDKREEATAEEKLISANEPSWKPWPQSRSQIRECVLNVKKLLVSLASMEWYCYALLGIITALMSFFMDMTVAKLLNAHQWLYGCLKGHHLLQFLCWTLYPACLCALSTSFAHSVCPYSAGSGVPEARAILLGVDMPDYLSLSNLFAKMFGLICTLAAGSTVFLGKVGPFVHLSIMVGAFMNRLHVSCRGGKQRAAKGEMLVVASAVGVASCFGAPISGVLFSIEVMGTHYSVSDYCPCFFAAACGAITFRLLSVCIGDQDTIQALFKTSFSTDLPFQPFEIIIFALLGLFCSILSCIYLLCHRWALRFVKTNKPISTFLATEKSLYSAIVAFLLACFTFYHSAGHFIASELTMRQLLTSMLDGTSWYSISQNISALPEPQNTFWQAWNPPGKSYFQTLTFFIITKVGLLVVACTLPLPAGYFMPVFVYGAAVGRFIGEGSFYLLSGGISSGKSLINPGGYALAGAAAFSGAVTHTLSPALLAVEMTGQCSYAVPALVATLVSNAVARAKHRPSFYDGISLIKRLPHLPSLIRACPKLADVQIKQFVIPAGAVLERSEKLISVQHILKESTDSELPVVESRDSSVLLGTINRSQLRECLLEHRNEDSCKQLEEVCCVQPVRLHLTPDSTVKQAHCIMSITGEQRLFITESGRLCGVITWKEMKKIIEKMAKEV

Target ORF information:

RefSeq Version	NM_200382.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel K (clcnk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200382.1

ORF Insert Sequence:

ATGGACGATA TTTGCGTAAA TGTAAAGCAA CCAGAAAATG AAAATGATGA ACAGCGTGTA 
CAAGAAGACA AGAGAGAGGA AGCTACAGCG GAAGAAAAGC TAATAAGTGC TAATGAGCCT 
TCATGGAAAC CATGGCCACA ATCACGCTCA CAAATCAGAG AGTGTGTGCT GAATGTGAAG 
AAGCTCCTGG TGTCTCTCGC CAGCATGGAG TGGTACTGTT ATGCTCTGTT AGGGATCATC 
ACAGCTCTAA TGAGCTTCTT CATGGACATG ACTGTGGCGA AGCTGCTAAA CGCCCACCAG 
TGGCTGTACG GCTGTCTGAA GGGTCACCAC CTGCTGCAGT TCCTCTGCTG GACGCTTTAC 
CCAGCCTGCC TCTGTGCTCT GTCCACCAGC TTCGCCCACA GCGTCTGCCC ATACTCTGCA 
GGCTCTGGTG TTCCTGAAGC GAGGGCTATT CTCTTAGGAG TGGACATGCC AGATTACCTG 
TCTCTCTCCA ATCTGTTTGC TAAAATGTTT GGCCTGATAT GCACACTAGC CGCTGGCAGC 
ACTGTGTTTC TTGGCAAAGT GGGCCCATTT GTCCATTTAT CTATCATGGT TGGTGCATTT 
ATGAATCGTC TGCATGTCTC CTGTCGTGGC GGAAAGCAGA GAGCAGCTAA AGGAGAAATG 
TTGGTTGTGG CATCAGCGGT GGGAGTTGCG AGCTGTTTTG GGGCTCCAAT AAGCGGCGTT 
CTCTTCAGCA TAGAGGTGAT GGGGACTCAT TATTCAGTCA GCGATTATTG TCCCTGCTTC 
TTTGCAGCGG CGTGCGGCGC CATCACCTTC AGGCTGCTTT CTGTCTGTAT TGGAGATCAA 
GACACAATCC AAGCCCTGTT TAAGACCAGC TTTTCTACTG ATCTGCCTTT TCAGCCTTTT 
GAAATAATAA TTTTTGCTCT GCTTGGGCTG TTTTGCAGTA TTCTCAGCTG TATTTACTTA 
CTCTGTCATC GCTGGGCTCT GCGTTTTGTC AAAACAAACA AACCCATCAG CACATTTTTG 
GCAACAGAAA AGTCACTTTA TTCAGCCATA GTGGCATTTC TTCTAGCCTG CTTTACATTT 
TATCACTCGG CGGGACATTT CATAGCTTCT GAGCTCACAA TGAGGCAGCT GCTTACTTCG 
ATGCTAGATG GCACTTCCTG GTATAGCATT TCCCAGAATA TATCTGCACT GCCTGAGCCA 
CAAAACACAT TCTGGCAGGC CTGGAATCCA CCAGGCAAAA GTTACTTCCA AACGCTGACA 
TTTTTCATTA TCACCAAGGT GGGGCTGCTG GTCGTGGCTT GTACTTTACC TTTACCTGCT 
GGTTATTTCA TGCCAGTATT TGTTTATGGT GCAGCAGTGG GTCGTTTTAT CGGCGAAGGC 
TCTTTCTATC TGCTCTCAGG TGGAATCTCA TCAGGGAAGA GCCTGATAAA CCCAGGCGGC 
TACGCTTTAG CAGGAGCGGC TGCTTTCTCT GGGGCCGTGA CACACACACT TTCTCCTGCC 
CTGCTGGCGG TGGAGATGAC CGGTCAGTGT TCCTATGCTG TTCCCGCTCT GGTGGCGACG 
CTCGTGTCCA ATGCTGTGGC TCGGGCTAAA CACAGGCCGT CTTTTTATGA TGGCATATCG 
CTTATTAAAC GGCTCCCGCA CCTGCCGTCT CTCATCCGCG CATGTCCGAA GTTGGCCGAT 
GTTCAGATTA AGCAGTTTGT GATCCCAGCA GGAGCTGTTC TGGAAAGGAG CGAGAAGCTG 
ATAAGTGTTC AGCACATCCT AAAGGAAAGC ACAGACTCTG AACTACCTGT GGTGGAGTCA 
CGCGACTCCT CTGTTCTCCT GGGCACAATC AACAGATCGC AGCTGCGCGA GTGTCTGCTT 
GAGCATAGAA ATGAGGATTC ATGTAAACAG CTTGAAGAAG TGTGTTGTGT TCAGCCTGTG 
CGTCTTCATC TGACTCCTGA CAGCACAGTA AAGCAGGCTC ATTGCATCAT GAGCATCACG 
GGTGAGCAGC GTCTGTTTAT AACAGAGAGT GGGCGACTGT GTGGCGTCAT CACATGGAAA 
GAGATGAAAA AAATTATTGA AAAGATGGCA AAGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clcnk ( XM_021469764.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325439.1 Danio rerio chloride channel K (clcnk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151451
Clone ID Related Accession (Same CDS sequence)	XM_021469764.1
Accession Version	XM_021469764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chloride channel protein ClC-Ka isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATA TTTGCGTAAA TGTAAAGCAA CCAGAAAATG AAAATGATGA ACAGCGTGTA 
CAAGAAGACA AGAGAGAGGA AGCTACAGCG GAAGAAAAGC TAATCAGTGC TAATGAGCCT 
TCATGGAAAC CATGGCCACA ATCACGCTCA CAAATCAGAG AGTGTGTGCT GAATGTGAAG 
AAGCTCCTGG TGTCTCTCGC CAGCATGGAG TGGTACTGTT ATGCTCTGTT AGGGATCATC 
ACAGCTCTAA TGAGCTTCTT CATGGACATG ACTGTGGCGA AGCTGCTAAA CGCCCACCAG 
TGGCTGTACG GCTGTCTGAA GGGTCACCAC CTGCTGCAGT TCCTCTGCTG GACGCTTTAC 
CCAGCCTGCC TCTGTGCTCT GTCCACCAGC TTCGCCCACA GCGTCTGCCC ATACTCTGCA 
GGCTCTGGTG TTCCTGAAGC GAGGGCTATT CTCTTAGGAG TGGACATGCC AGATTACCTG 
TCTCTCTCCA ATCTGTTTGC TAAAATGTTT GGCCTGATAT GCACACTAGC CGCTGGCAGC 
ACTGTGTTTC TTGGCAAAGT GGGCCCATTT GTCCATTTAT CTATCATGGT TGGTGCATTT 
ATGAACCGTC TGCATGTCTC CTGTCGTGGC GGAAAGCAGA GAGCAGCTAA AGGAGAAATG 
TTGGTTGTGG CATCAGCGGT GGGAGTTGCG AGCTGTTTTG GGGCTCCAAT AAGCGGCGTT 
CTCTTCAGTA TAGAGGTGAT GGGGACTCAT TATTCAGTCA GCGATTATTG CCCCTGCTTC 
TTTGCAGCGG CGTGCGGCGC CATCACCTTC AGGCTGCTTT CTGTCTGTAT TGGAGATCAA 
GACACAATCC AAGCCCTGTT TAAGACCAGC TTTTCTACTG ATCTGCCTTT TCAGCCTTTT 
GAAATAATAA TTTTTGCTCT GCTTGGGCTG TTTTGCAGTA TTCTCAGCTG TATTTACTTA 
CTCTGTCATC GCTGGGCTCT GCGTTTTGTC AAAACAAACA AACCCATCAG CACATTTTTG 
GCAACAGAAA AGTCACTTTA TTCAGCCATA GTGGCATTTC TTCTAGCCTG CTTTACGTTT 
TATCACTCGG CGGGACATTT CATAGCTTCT GAGCTCACAA TGAGGCAGCT GCTTACTTCG 
ATGCTAGATG GCACTTCCTG GTATAACATT TCCCAGAATA TATCTGCACT GCCTGAGCCA 
CAAAACACAT TCTGGCAGGC CTGGAATCCA CCAGGCAAAA GTTACTTCCA AACGCTGACA 
TTTTTCATTA TCACCAAGGT GGGGCTGCTG GTCGTGGCTT GTACTTTACC TTTACCTGCT 
GGTTATTTCA TGCCAGTATT TGTTTATGGT GCAGCAGTGG GCCGTTTTAT CGGCGAAGGC 
TCTTTCTATC TGCTCTCAGG TGGAATCTCA TCAGGGAAGA GCCTGATAAA CCCAGGCGGC 
TACGCTTTAG CAGGAGCGGC TGCTTTCTCC GGGGCCGTGA CACACACACT TTCTCCTGCC 
CTGCTGGCGG TGGAGATGAC CGGTCAGTGT TCCTATGCTG TTCCCGCTCT GGTGGCGACG 
CTCGTGTCCA ATGCTGTGGC TCGGGCTAAA CACAGGCCGT CTTTCTATGA TGGCATATCG 
CTTATTAAAC GGCTCCCGCA CCTGCCGTCT CTAATCCGCG CATGTCCGAA GTTGGCCGAT 
GTTCAGATTA AGCAGTTTGT GATCCCAGCA GGAGCTGTTC TGGAAAGGAG CGAGAAGCTG 
ATAAGTGTTC AGCACATCCT AAAGGAAAGC ACAGACTCTG AACTACCTGT GGTGGAGTCA 
CGCGACTTCT CTGTTCTCCT GGGCACAATC AACAGATCGC AGTTGCGCGA GTGTCTGCTT 
GAGCATAGAA ATGAGGATTC ATGTAAACAG CTTGAAGAAG TGTGTTGTGT TCAGCCTGTG 
CGTCTTCATC TGACTCCTGA CAGCACAGTA AAGCAGGCTC ATTGCATCAT GAGCATCACG 
GGTGAGCAGC GTCTGTTTAT AACAGAGAGT GGGCGACTGT GTGGCGTCAT CACATGGAAA 
GAGGTGACAA GAGCAGCCTG CAAGAACAAA CATAAACACA TCCACACACA CATCTATACT 
CCAATCAAAT ACACCAATGA ACACAGAGCT CATACATTAC AATTCATTAT AAAATCTGAT 
TTTTCTTCCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325439.1
CDS	559..2733
Translation	MDDICVNVKQPENENDEQRVQEDKREEATAEEKLISANEPSWKPWPQSRSQIRECVLNVKKLLVSLASMEWYCYALLGIITALMSFFMDMTVAKLLNAHQWLYGCLKGHHLLQFLCWTLYPACLCALSTSFAHSVCPYSAGSGVPEARAILLGVDMPDYLSLSNLFAKMFGLICTLAAGSTVFLGKVGPFVHLSIMVGAFMNRLHVSCRGGKQRAAKGEMLVVASAVGVASCFGAPISGVLFSIEVMGTHYSVSDYCPCFFAAACGAITFRLLSVCIGDQDTIQALFKTSFSTDLPFQPFEIIIFALLGLFCSILSCIYLLCHRWALRFVKTNKPISTFLATEKSLYSAIVAFLLACFTFYHSAGHFIASELTMRQLLTSMLDGTSWYNISQNISALPEPQNTFWQAWNPPGKSYFQTLTFFIITKVGLLVVACTLPLPAGYFMPVFVYGAAVGRFIGEGSFYLLSGGISSGKSLINPGGYALAGAAAFSGAVTHTLSPALLAVEMTGQCSYAVPALVATLVSNAVARAKHRPSFYDGISLIKRLPHLPSLIRACPKLADVQIKQFVIPAGAVLERSEKLISVQHILKESTDSELPVVESRDFSVLLGTINRSQLRECLLEHRNEDSCKQLEEVCCVQPVRLHLTPDSTVKQAHCIMSITGEQRLFITESGRLCGVITWKEVTRAACKNKHKHIHTHIYTPIKYTNEHRAHTLQFIIKSDFSSR

Target ORF information:

RefSeq Version	XM_021469764.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel K (clcnk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469764.1

ORF Insert Sequence:

ATGGACGATA TTTGCGTAAA TGTAAAGCAA CCAGAAAATG AAAATGATGA ACAGCGTGTA 
CAAGAAGACA AGAGAGAGGA AGCTACAGCG GAAGAAAAGC TAATCAGTGC TAATGAGCCT 
TCATGGAAAC CATGGCCACA ATCACGCTCA CAAATCAGAG AGTGTGTGCT GAATGTGAAG 
AAGCTCCTGG TGTCTCTCGC CAGCATGGAG TGGTACTGTT ATGCTCTGTT AGGGATCATC 
ACAGCTCTAA TGAGCTTCTT CATGGACATG ACTGTGGCGA AGCTGCTAAA CGCCCACCAG 
TGGCTGTACG GCTGTCTGAA GGGTCACCAC CTGCTGCAGT TCCTCTGCTG GACGCTTTAC 
CCAGCCTGCC TCTGTGCTCT GTCCACCAGC TTCGCCCACA GCGTCTGCCC ATACTCTGCA 
GGCTCTGGTG TTCCTGAAGC GAGGGCTATT CTCTTAGGAG TGGACATGCC AGATTACCTG 
TCTCTCTCCA ATCTGTTTGC TAAAATGTTT GGCCTGATAT GCACACTAGC CGCTGGCAGC 
ACTGTGTTTC TTGGCAAAGT GGGCCCATTT GTCCATTTAT CTATCATGGT TGGTGCATTT 
ATGAACCGTC TGCATGTCTC CTGTCGTGGC GGAAAGCAGA GAGCAGCTAA AGGAGAAATG 
TTGGTTGTGG CATCAGCGGT GGGAGTTGCG AGCTGTTTTG GGGCTCCAAT AAGCGGCGTT 
CTCTTCAGTA TAGAGGTGAT GGGGACTCAT TATTCAGTCA GCGATTATTG CCCCTGCTTC 
TTTGCAGCGG CGTGCGGCGC CATCACCTTC AGGCTGCTTT CTGTCTGTAT TGGAGATCAA 
GACACAATCC AAGCCCTGTT TAAGACCAGC TTTTCTACTG ATCTGCCTTT TCAGCCTTTT 
GAAATAATAA TTTTTGCTCT GCTTGGGCTG TTTTGCAGTA TTCTCAGCTG TATTTACTTA 
CTCTGTCATC GCTGGGCTCT GCGTTTTGTC AAAACAAACA AACCCATCAG CACATTTTTG 
GCAACAGAAA AGTCACTTTA TTCAGCCATA GTGGCATTTC TTCTAGCCTG CTTTACGTTT 
TATCACTCGG CGGGACATTT CATAGCTTCT GAGCTCACAA TGAGGCAGCT GCTTACTTCG 
ATGCTAGATG GCACTTCCTG GTATAACATT TCCCAGAATA TATCTGCACT GCCTGAGCCA 
CAAAACACAT TCTGGCAGGC CTGGAATCCA CCAGGCAAAA GTTACTTCCA AACGCTGACA 
TTTTTCATTA TCACCAAGGT GGGGCTGCTG GTCGTGGCTT GTACTTTACC TTTACCTGCT 
GGTTATTTCA TGCCAGTATT TGTTTATGGT GCAGCAGTGG GCCGTTTTAT CGGCGAAGGC 
TCTTTCTATC TGCTCTCAGG TGGAATCTCA TCAGGGAAGA GCCTGATAAA CCCAGGCGGC 
TACGCTTTAG CAGGAGCGGC TGCTTTCTCC GGGGCCGTGA CACACACACT TTCTCCTGCC 
CTGCTGGCGG TGGAGATGAC CGGTCAGTGT TCCTATGCTG TTCCCGCTCT GGTGGCGACG 
CTCGTGTCCA ATGCTGTGGC TCGGGCTAAA CACAGGCCGT CTTTCTATGA TGGCATATCG 
CTTATTAAAC GGCTCCCGCA CCTGCCGTCT CTAATCCGCG CATGTCCGAA GTTGGCCGAT 
GTTCAGATTA AGCAGTTTGT GATCCCAGCA GGAGCTGTTC TGGAAAGGAG CGAGAAGCTG 
ATAAGTGTTC AGCACATCCT AAAGGAAAGC ACAGACTCTG AACTACCTGT GGTGGAGTCA 
CGCGACTTCT CTGTTCTCCT GGGCACAATC AACAGATCGC AGTTGCGCGA GTGTCTGCTT 
GAGCATAGAA ATGAGGATTC ATGTAAACAG CTTGAAGAAG TGTGTTGTGT TCAGCCTGTG 
CGTCTTCATC TGACTCCTGA CAGCACAGTA AAGCAGGCTC ATTGCATCAT GAGCATCACG 
GGTGAGCAGC GTCTGTTTAT AACAGAGAGT GGGCGACTGT GTGGCGTCAT CACATGGAAA 
GAGGTGACAA GAGCAGCCTG CAAGAACAAA CATAAACACA TCCACACACA CATCTATACT 
CCAATCAAAT ACACCAATGA ACACAGAGCT CATACATTAC AATTCATTAT AAAATCTGAT 
TTTTCTTCCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

Comparative transcriptomic analysis identifies evolutionarily conserved gene products in the vertebrate renal distal convoluted tubule.
Pflugers Archiv : European journal of physiology469(7-8)859-867(2017 Aug)
Sugano Y,Cianciolo Cosentino C,Loffing-Cueni D,Neuhauss SCF,Loffing J

Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis.
International journal of biological sciences11(10)1190-203(2015)
Wang YF,Yan JJ,Tseng YC,Chen RD,Hwang PP

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clcnk cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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