chuk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chuk
Entrez Gene ID	393287
Full Name	conserved helix-loop-helix ubiquitous kinase
Synonyms	ikk1,zgc:56539
General protein information	Preferred Names conserved helix-loop-helix ubiquitous kinase Names inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase 1 IKK-A IKK-alpha IKKa NFKBIKA i kappa-B kinase alpha ikBKA ikappaB kinase nuclear factor NF-kappa-B inhibitor kinase alpha pky
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200317.1	NP_956611.1	inhibitor of nuclear factor kappa-B kinase subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHUK	NP_001269.3
	CHUK	XP_001107171.1
Canis lupus familiaris (dog)	CHUK	XP_534990.2
Gallus gallus (chicken)	CHUK	NP_001012922.1
Bos taurus (cattle)	CHUK	NP_776446.1
Danio rerio (zebrafish)	chuk	NP_956611.1
Xenopus tropicalis (tropical clawed frog)	chuk	NP_001016900.1
Pan troglodytes (chimpanzee)	CHUK	XP_001167720.1
Mus musculus (house mouse)	Chuk	NP_031726.2
Rattus norvegicus (Norway rat)	Chuk	NP_001101058.1

Gene Ontology
Bibliography

Related articles in PubMed

Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio).
Zhang CN, Zhang JL, Huang Y, Ren HT, Guan SH, Zeng QH
Environmental toxicology33(1)104-111(2018 Jan)

IKK? regulates human keratinocyte migration through surveillance of the redox environment.
Lisse TS, Rieger S
Journal of cell science130(5)975-988(2017 03)

D?az-Pascual F, Ort?z-Sever?n J, Varas MA, Allende ML, Ch?vez FP
Frontiers in cellular and infection microbiology7334(2017)

The defensive effect of phellodendrine against AAPH-induced oxidative stress through regulating the AKT/NF-?B pathway in zebrafish embryos.
Li L, Huang T, Tian C, Xiao Y, Kou S, Zhou X, Liu S, Ye X, Li X
Life sciences15797-106(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings reveal a molecular mechanism regulating migration and invasion of epithelial cells and establish a key direct link between IKKbeta and cell motility controlled by Rap-integrin signaling.
Title: Control of epithelial cell migration and invasion by the IKK?- and CK1?-mediated degradation of RAPGEF2.

poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis
Title: poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis.

Ikk1 negatively regulates NF-kappaB by sequestering NEMO from active IKK complexes, indicating that IKK1 can function as a repressor of NF-kappaB.
Title: Zebrafish IkappaB kinase 1 negatively regulates NF-kappaB activity.

The following chuk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chuk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00293	NM_200317.1 Latest version!	Danio rerio component of inhibitor of nuclear factor kappa B kinase complex (chuk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chuk ( NM_200317.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956611.1 Danio rerio component of inhibitor of nuclear factor kappa B kinase complex (chuk), mRNA.

Price & Availability↑

CloneID	ODa00293
Clone ID Related Accession (Same CDS sequence)	NM_200317.1
Accession Version	NM_200317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Danio rerio(zebrafish)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC051614.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051614.1, AY735397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAAC CCCCTTTCAG ACAGAATCAG GTCTGTGGCT CATGGGTTAT GAAGGAGAGG 
CTTGGAACAG GAGGATTTGG CCATGTCTAT TTGTATCAGA ATCAGGAAAC ATCAGAAAAA 
ATAGCTGTGA AGCTCTGTCG CCTTGAGCTT AATTCAAAAA ACAAAGACAG ATGGAGTCGA 
GAGATCCAGA TTATGAAAAA GCTAAAGCAC CTTAATGTGG TCACAGCAAA GGATGTGCCT 
GAGGAGATGA TGCACATTGC CTTGAATGAT TTACCACTTT TAGCCATGGA GTATTGTTCC 
AAAGGAGATC TTCGTAAGTT GTTGAGCAAA CCTGAAAATT GCTGTGGGTT GAAAGAGAGT 
GAGGTGTTGT CTCTACTCAA TGATGTTGGT TCTGGAATCC AGTACCTTCA TGAGAATAAG 
ATCATACACA GGGATCTCAA GCCAGAGAAT ATTGTGCTGC AAGAAATTAA TGGAAAGCTT 
GTTCACAAGA TAATCGACCT TGGTTATGCC AAAGACCTGG ACCAGGGGAG CTTATGCACT 
TCATTTGTGG GGACTTTACA ATATCTGGCT CCAGAGCTTT TTGAAGGCAA GTCCTACACA 
GTCACTGTGG ACTTTTGGAG CTTTGGCACC ATGATCTTTG AATGCTGCTG TGGATTTCGT 
CCATTTCTTC ACAATCTTCA GCCTGTGCAG TGGACAAGCA AAGTGCGAAA TAAAGGTCCG 
AAGGATATAA TGGCTGTGGA AGATATGAAT GGCGAGGTCC GCTTTTCCAC CCACCTTCCA 
TACCCAAACA ATCTTAGCAG GACACTACTG GAGCCCTTGG AAGGCCTTTT ACAGCTGATG 
CTCAAATGGG ACCCTGTACA GAGAGGATTA GGACTGAACA CAGATTCAAA ACAGCCCCAG 
TGCTTTGTTC TTCTAGATCA AATACTGAGC ATGAAGGTTG TGCACATCTT GAACATGACT 
ACGACCCAGG TTCACTCATT TTTGCTAAGC CCAGATGAAG GGCTTCACTC TCTGCAACAG 
AGGATTGAGA ACGAAACCAA AATTGAACTT TTGAATCAAG ATCTTCTGCA AGAAACAGGA 
GTCATGTTAG ATCCCAGGAA ACCTGCTGCA CAGTGTGTGT TAGATGGAGT AAGAGGATGG 
GACAGCTACA TTGTATATCT GTTTGACAAG AGTTTGACCA AGTACATGGG ACCTTTAACT 
GCTAGAACAC TTCCCGAAAG TGTCAACTTT ATTGTTCGGG AAACCAAAAC TCAGCTCCCA 
CTGAGTACCC TAAAAAAGGT TTGGGGTGAG GCGGTTAGCT ACATTTGTGG CCCCAGAGAA 
GACTACAGCC GCCTCTTCCA GGGCCAGCGT GCAGCCATGC TGAGTCTTCT TCGCTTCAAT 
ACAAATTTGA CAAGATACAA GAACATGATG TTCTCCTTTT CACAACAGCT CAAAGCAAAG 
CTGGACTTTT TTAAGACCAG CATACAGTAC GACTTGGAGA AATACAGTGA TCAGATGCAG 
TACGGCATAT CTTCAGAAAA GATGCTGAAG GCTTGGCACG AAAATGAAGA GAGGGCTGCT 
GCTTTTGCAC AGGTGGCAGA GATTGGTCAT CTGGATGAGG AAATAATGGC GTTGCATTCG 
GAAATCGTTG AGTTACAGAG AAGTCCTTAT GCTCGTCGTC AAGGAGATGT CATGGAGCAG 
TTGCAAGAGA AAGCCATTGA ACTCTACAAA CAGTTGAAGG CAAAATGCAA AATGCCAGAC 
CCTCAGCATG GCTACAGCGA CAGCTCTGAG ATGGTGAAGG TCATAGTACA GACTGTCCAG 
AATCAAGACC GGGTGCTAAG AGATCTATAT GCACACCTCA GTAAAATCCC TCTGAGTAAG 
CAGAAGATCA TCGACCTCTT CCCTAAAATC GAGAGAACGC TGGAATGCAT AAAGGAGGCA 
GACACCACAG TCATGCAGAT GCAGATCAAG AGACAGAGAG AGTTCTGGCA CTTACTGAAG 
ATTGCCTGTG CACAAAACAC CACACGGAGC TCAGTGAGTC AGAGCGGAGA AATGCCCTCC 
TCGCTGTCTA CTTGGAACCA GACTCAAGCC CAGTGTTCCT CCCGTCTCCC CATGTCCTTG 
CAAGTGCCAC ACGAGGGTGA TTCAGTTAAT CATTTACTGG AGGAGAATCA GCGTTACTTG 
ACTCAGCTCA CCAGTTTACT GCAAGAAACC ACCGAGGAGA AATCAGAGAG CATAATGGCT 
CAGGACTGGA GCTGGACAAA ATACGAATCC CTGGTTGCTA AATCAGCGCG TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956611.1
CDS	123..2399
Translation	MEKPPFRQNQVCGSWVMKERLGTGGFGHVYLYQNQETSEKIAVKLCRLELNSKNKDRWSREIQIMKKLKHLNVVTAKDVPEEMMHIALNDLPLLAMEYCSKGDLRKLLSKPENCCGLKESEVLSLLNDVGSGIQYLHENKIIHRDLKPENIVLQEINGKLVHKIIDLGYAKDLDQGSLCTSFVGTLQYLAPELFEGKSYTVTVDFWSFGTMIFECCCGFRPFLHNLQPVQWTSKVRNKGPKDIMAVEDMNGEVRFSTHLPYPNNLSRTLLEPLEGLLQLMLKWDPVQRGLGLNTDSKQPQCFVLLDQILSMKVVHILNMTTTQVHSFLLSPDEGLHSLQQRIENETKIELLNQDLLQETGVMLDPRKPAAQCVLDGVRGWDSYIVYLFDKSLTKYMGPLTARTLPESVNFIVRETKTQLPLSTLKKVWGEAVSYICGPREDYSRLFQGQRAAMLSLLRFNTNLTRYKNMMFSFSQQLKAKLDFFKTSIQYDLEKYSDQMQYGISSEKMLKAWHENEERAAAFAQVAEIGHLDEEIMALHSEIVELQRSPYARRQGDVMEQLQEKAIELYKQLKAKCKMPDPQHGYSDSSEMVKVIVQTVQNQDRVLRDLYAHLSKIPLSKQKIIDLFPKIERTLECIKEADTTVMQMQIKRQREFWHLLKIACAQNTTRSSVSQSGEMPSSLSTWNQTQAQCSSRLPMSLQVPHEGDSVNHLLEENQRYLTQLTSLLQETTEEKSESIMAQDWSWTKYESLVAKSARL

Target ORF information:

RefSeq Version	NM_200317.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio component of inhibitor of nuclear factor kappa B kinase complex (chuk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200317.1

ORF Insert Sequence:

ATGGAGAAAC CCCCTTTCAG ACAGAATCAG GTCTGTGGCT CATGGGTTAT GAAGGAGAGG 
CTTGGAACAG GAGGATTTGG CCATGTCTAT TTGTATCAGA ATCAGGAAAC ATCAGAAAAA 
ATAGCTGTGA AGCTCTGTCG CCTTGAGCTT AATTCAAAAA ACAAAGACAG ATGGAGTCGA 
GAGATCCAGA TTATGAAAAA GCTAAAGCAC CTTAATGTGG TCACAGCAAA GGATGTGCCT 
GAGGAGATGA TGCACATTGC CTTGAATGAT TTACCACTTT TAGCCATGGA GTATTGTTCC 
AAAGGAGATC TTCGTAAGTT GTTGAGCAAA CCTGAAAATT GCTGTGGGTT GAAAGAGAGT 
GAGGTGTTGT CTCTACTCAA TGATGTTGGT TCTGGAATCC AGTACCTTCA TGAGAATAAG 
ATCATACACA GGGATCTCAA GCCAGAGAAT ATTGTGCTGC AAGAAATTAA TGGAAAGCTT 
GTTCACAAGA TAATCGACCT TGGTTATGCC AAAGACCTGG ACCAGGGGAG CTTATGCACT 
TCATTTGTGG GGACTTTACA ATATCTGGCT CCAGAGCTTT TTGAAGGCAA GTCCTACACA 
GTCACTGTGG ACTTTTGGAG CTTTGGCACC ATGATCTTTG AATGCTGCTG TGGATTTCGT 
CCATTTCTTC ACAATCTTCA GCCTGTGCAG TGGACAAGCA AAGTGCGAAA TAAAGGTCCG 
AAGGATATAA TGGCTGTGGA AGATATGAAT GGCGAGGTCC GCTTTTCCAC CCACCTTCCA 
TACCCAAACA ATCTTAGCAG GACACTACTG GAGCCCTTGG AAGGCCTTTT ACAGCTGATG 
CTCAAATGGG ACCCTGTACA GAGAGGATTA GGACTGAACA CAGATTCAAA ACAGCCCCAG 
TGCTTTGTTC TTCTAGATCA AATACTGAGC ATGAAGGTTG TGCACATCTT GAACATGACT 
ACGACCCAGG TTCACTCATT TTTGCTAAGC CCAGATGAAG GGCTTCACTC TCTGCAACAG 
AGGATTGAGA ACGAAACCAA AATTGAACTT TTGAATCAAG ATCTTCTGCA AGAAACAGGA 
GTCATGTTAG ATCCCAGGAA ACCTGCTGCA CAGTGTGTGT TAGATGGAGT AAGAGGATGG 
GACAGCTACA TTGTATATCT GTTTGACAAG AGTTTGACCA AGTACATGGG ACCTTTAACT 
GCTAGAACAC TTCCCGAAAG TGTCAACTTT ATTGTTCGGG AAACCAAAAC TCAGCTCCCA 
CTGAGTACCC TAAAAAAGGT TTGGGGTGAG GCGGTTAGCT ACATTTGTGG CCCCAGAGAA 
GACTACAGCC GCCTCTTCCA GGGCCAGCGT GCAGCCATGC TGAGTCTTCT TCGCTTCAAT 
ACAAATTTGA CAAGATACAA GAACATGATG TTCTCCTTTT CACAACAGCT CAAAGCAAAG 
CTGGACTTTT TTAAGACCAG CATACAGTAC GACTTGGAGA AATACAGTGA TCAGATGCAG 
TACGGCATAT CTTCAGAAAA GATGCTGAAG GCTTGGCACG AAAATGAAGA GAGGGCTGCT 
GCTTTTGCAC AGGTGGCAGA GATTGGTCAT CTGGATGAGG AAATAATGGC GTTGCATTCG 
GAAATCGTTG AGTTACAGAG AAGTCCTTAT GCTCGTCGTC AAGGAGATGT CATGGAGCAG 
TTGCAAGAGA AAGCCATTGA ACTCTACAAA CAGTTGAAGG CAAAATGCAA AATGCCAGAC 
CCTCAGCATG GCTACAGCGA CAGCTCTGAG ATGGTGAAGG TCATAGTACA GACTGTCCAG 
AATCAAGACC GGGTGCTAAG AGATCTATAT GCACACCTCA GTAAAATCCC TCTGAGTAAG 
CAGAAGATCA TCGACCTCTT CCCTAAAATC GAGAGAACGC TGGAATGCAT AAAGGAGGCA 
GACACCACAG TCATGCAGAT GCAGATCAAG AGACAGAGAG AGTTCTGGCA CTTACTGAAG 
ATTGCCTGTG CACAAAACAC CACACGGAGC TCAGTGAGTC AGAGCGGAGA AATGCCCTCC 
TCGCTGTCTA CTTGGAACCA GACTCAAGCC CAGTGTTCCT CCCGTCTCCC CATGTCCTTG 
CAAGTGCCAC ACGAGGGTGA TTCAGTTAAT CATTTACTGG AGGAGAATCA GCGTTACTTG 
ACTCAGCTCA CCAGTTTACT GCAAGAAACC ACCGAGGAGA AATCAGAGAG CATAATGGCT 
CAGGACTGGA GCTGGACAAA ATACGAATCC CTGGTTGCTA AATCAGCGCG TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio).
Environmental toxicology33(1)104-111(2018 Jan)
Zhang CN,Zhang JL,Huang Y,Ren HT,Guan SH,Zeng QH

IKK? regulates human keratinocyte migration through surveillance of the redox environment.
Journal of cell science130(5)975-988(2017 03)
Lisse TS,Rieger S

Frontiers in cellular and infection microbiology7334(2017)
D?az-Pascual F,Ort?z-Sever?n J,Varas MA,Allende ML,Ch?vez FP

The defensive effect of phellodendrine against AAPH-induced oxidative stress through regulating the AKT/NF-?B pathway in zebrafish embryos.
Life sciences15797-106(2016 Jul)
Li L,Huang T,Tian C,Xiao Y,Kou S,Zhou X,Liu S,Ye X,Li X

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chuk cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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