mkks cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkks
Entrez Gene ID	393134
Full Name	McKusick-Kaufman syndrome
Synonyms	bbs6,zgc:55608
General protein information	Preferred Names McKusick-Kaufman syndrome Names McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin Bardet-Biedl syndrome 6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307468.2	XP_009305743.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
NM_200165.1	NP_956459.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin
XM_021480540.1	XP_021336215.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
XM_021480541.1	XP_021336216.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MKKS	XP_001169410.1
Canis lupus familiaris (dog)	MKKS	XP_542894.1
Rattus norvegicus (Norway rat)	Mkks	NP_001008354.1
Homo sapiens (human)	MKKS	NP_740754.1
Mus musculus (house mouse)	Mkks	NP_067502.2
Danio rerio (zebrafish)	mkks	NP_956459.1
Xenopus tropicalis (tropical clawed frog)	mkks	XP_002931783.1
	MKKS	XP_001116196.1
Bos taurus (cattle)	MKKS	NP_001193100.1
Gallus gallus (chicken)	MKKS	NP_001186339.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein.
Scott CA, Marsden AN, Rebagliati MR, Zhang Q, Chamling X, Searby CC, Baye LM, Sheffield VC, Slusarski DC
PLoS genetics13(7)e1006936(2017 Jul)

Combining Cep290 and Mkks ciliopathy alleles in mice rescues sensory defects and restores ciliogenesis.
Rachel RA, May-Simera HL, Veleri S, Gotoh N, Choi BY, Murga-Zamalloa C, McIntyre JC, Marek J, Lopez I, Hackett AN, Zhang J, Brooks M, den Hollander AI, Beales PL, Li T, Jacobson SG, Sood R, Martens JR, Liu P, Friedman TB, Khanna H, Koenekoop RK, Kelley MW, Swaroop A
The Journal of clinical investigation122(4)1233-45(2012 Apr)

TTC21B contributes both causal and modifying alleles across the ciliopathy spectrum.
Davis EE, Zhang Q, Liu Q, Diplas BH, Davey LM, Hartley J, Stoetzel C, Szymanska K, Ramaswami G, Logan CV, Muzny DM, Young AC, Wheeler DA, Cruz P, Morgan M, Lewis LR, Cherukuri P, Maskeri B, Hansen NF, Mullikin JC, Blakesley RW, Bouffard GG, , Gyapay G, Rieger S, T?nshoff B, Kern I, Soliman NA, Neuhaus TJ, Swoboda KJ, Kayserili H, Gallagher TE, Lewis RA, Bergmann C, Otto EA, Saunier S, Scambler PJ, Beales PL, Gleeson JG, Maher ER, Atti?-Bitach T, Dollfus H, Johnson CA, Green ED, Gibbs RA, Hildebrandt F, Pierce EA, Katsanis N
Nature genetics43(3)189-96(2011 Mar)

Functional analyses of variants reveal a significant role for dominant negative and common alleles in oligogenic Bardet-Biedl syndrome.
Zaghloul NA, Liu Y, Gerdes JM, Gascue C, Oh EC, Leitch CC, Bromberg Y, Binkley J, Leibel RL, Sidow A, Badano JL, Katsanis N
Proceedings of the National Academy of Sciences of the United States of America107(23)10602-7(2010 Jun)

Genetic interaction between Bardet-Biedl syndrome genes and implications for limb patterning.
Tayeh MK, Yen HJ, Beck JS, Searby CC, Westfall TA, Griesbach H, Sheffield VC, Slusarski DC
Human molecular genetics17(13)1956-67(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We demonstrate that through this interaction, BBS6 modulates the sub-cellular localization of SMARCC1 and find, by transcriptional profiling, similar transcriptional changes following smarcc1a and bbs6 manipulation. Our work identifies a new function for BBS6 in nuclear-cytoplasmic transport, and provides insight into the disease mechanism underlying the congenital heart defects in McKusick-Kaufman syndrome patients.
Title: Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein.

The following mkks gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkks cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35339	NM_200165.1 Latest version!	Danio rerio McKusick-Kaufman syndrome (mkks), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40376	XM_009307468.2 Latest version!	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40376	XM_021480540.1 Latest version!	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa148495	XM_021480541.1 Latest version!	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkks ( NM_200165.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956459.1 Danio rerio McKusick-Kaufman syndrome (mkks), mRNA.

Price & Availability↑

CloneID	ODa35339
Clone ID Related Accession (Same CDS sequence)	NM_200165.1
Accession Version	NM_200165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-20
Organism	Danio rerio(zebrafish)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045401.1. On Jul 6, 2005 this sequence version replaced XM_697147.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCCACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTAACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CACGCAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ATGCAGCAGA TCAGTTCATT GATCACACAG 
GCCTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACAGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTTTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGCTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGATG ACTTGCCAGA GAGCAGAACA TGTGTTGCGA 
CTGACTCTGA GGGAGCCCTA CGCCCTGCTT GGAGGCGGCT GCACAGAAAC ACAGCTGGCC 
ACCCACATCT CACACATGAA TCAGTCCACA GCTCCAACCA CTGCTGCAGC TCTGGGTATT 
TCTCATTCAG AGTTCCTGAT GGCAGTCGAG TCATTCCGCA GTTCTTTACT TGCTGTGGCT 
CTTTCCTTAG AGCATGATGG ACAGGACTGT CTCATTGACC TGACATATGG CCACAGATGG 
GTCATGGATA TCAGTTCTCA GACAGAAGTT AAGCACACCT GTGGTTGTGG ACTGCTGGAG 
GACAGATCCA ACCTGGAGAA GACTCATCTA AATACAGCTT GTCAGACTTT TTCTCCTGTT 
TTCCTAGAAA ACAGTGACAC TCAGCCTAAA GTATTAGATT CCTTTTCTGC CAAGCTTAAT 
GCGTTGAATG TGGCAGTTGA GATGGCTAGT TTGGTTCTTG ATGTAAAATA TATAATCAAG 
GATGTTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956459.1
CDS	69..1760
Translation	MSRISKKKPALCTDEPLSNSTICQKITLLRNILSTAYGPTGRLKQIHNNVGGHVLTTSTSTALLKRLEMSEPLLKLISTALQHHTTRYSDSGLFMGIFTLTLIENTKKYGLRTSTAIKVYKHLVEQCNVYLKGDSCGCKVPVEFSSCDSLVALARSMITSKPACMLDSREMQQISSLITQAFLYSIPCNSSGTACFGRTVTIGIEGQSVNHSSVFPGLLLDVPEMLLPGDLERLGDGPFKVVLFGVSLSGDISEVGDVALEVHRGLNPERDLLQQLLKIGEQAVKDKVSLFACQKVVHPVLQHYLREHEVVVIERLGLALMEPFAKITGARAVASLFSLVPMEAYGLVAGLCFQDCGSKKLLQLLSSKHAAISTMVLCHRNETMLEELKMTCQRAEHVLRLTLREPYALLGGGCTETQLATHISHMNQSTAPTTAAALGISHSEFLMAVESFRSSLLAVALSLEHDGQDCLIDLTYGHRWVMDISSQTEVKHTCGCGLLEDRSNLEKTHLNTACQTFSPVFLENSDTQPKVLDSFSAKLNALNVAVEMASLVLDVKYIIKDVN

Target ORF information:

RefSeq Version	NM_200165.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio McKusick-Kaufman syndrome (mkks), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200165.1

ORF Insert Sequence:

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCCACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTAACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CACGCAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ATGCAGCAGA TCAGTTCATT GATCACACAG 
GCCTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACAGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTTTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGCTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGATG ACTTGCCAGA GAGCAGAACA TGTGTTGCGA 
CTGACTCTGA GGGAGCCCTA CGCCCTGCTT GGAGGCGGCT GCACAGAAAC ACAGCTGGCC 
ACCCACATCT CACACATGAA TCAGTCCACA GCTCCAACCA CTGCTGCAGC TCTGGGTATT 
TCTCATTCAG AGTTCCTGAT GGCAGTCGAG TCATTCCGCA GTTCTTTACT TGCTGTGGCT 
CTTTCCTTAG AGCATGATGG ACAGGACTGT CTCATTGACC TGACATATGG CCACAGATGG 
GTCATGGATA TCAGTTCTCA GACAGAAGTT AAGCACACCT GTGGTTGTGG ACTGCTGGAG 
GACAGATCCA ACCTGGAGAA GACTCATCTA AATACAGCTT GTCAGACTTT TTCTCCTGTT 
TTCCTAGAAA ACAGTGACAC TCAGCCTAAA GTATTAGATT CCTTTTCTGC CAAGCTTAAT 
GCGTTGAATG TGGCAGTTGA GATGGCTAGT TTGGTTCTTG ATGTAAAATA TATAATCAAG 
GATGTTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkks ( XM_009307468.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305743.1 Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40376
Clone ID Related Accession (Same CDS sequence)	XM_009307468.2 , XM_021480540.1
Accession Version	XM_009307468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGGTT TGTGCAATGA CTTGCCAGAG AGCAGAACAT 
GTGTTGCGAC TGACTCTGAG GGAGCCCTAC GCCCTGCTTG GAGGCGGCTG CACAGAAACA 
CAGCTGGCCA CCCACATCAC ACATATGAAT CAGTCCACAG CTCCAACCAC TGCTGCAGCT 
CTGGGTATTT CCCATTCAGA GTTCCTGATG GCAGTCGAGT CATTTCGCAG TTCTTTACTT 
GCTGTGGCTC TTTCCTTAGA GCATGATGGA CAGGACTGTC TCATTGACCT GACATATGGC 
CACAGATGGG TCATGGATAT CAGTTCTCAG ACAGAAGTTA AGCACACCTG TGGTTGTGGA 
CTGCTGGAGG ACAGATCCAA CCTGGAGAAG ACTCATCTAA ATACAGCTTG TCAGACTTTT 
TCTCCTGTTT TCCTAGAAAA CAATGACACT CAGCCTAAAG TATTAGATTC CTTTTCTGCC 
AAGCTTAATG CGTTGAATGT GGCAGTTGAG ATGGCTAGTT TGGTTCTTGA TGTAAAATAT 
ATAATCAAGG ATGTTAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305743.1
CDS	85..1785
Translation	MSRISKKKPALCTDEPLSNSTICQKITLLRNILSTAYGPTGRLKQIHNNVGGHVLTTSTSTALLKRLEMSEPLLKLISTALQHHTTRYSDSGLFMGIFTLTLIENTKKYGLRTSTAIKVYKHLVEQCNVYLKGDSCGCKVPVEFSSCDSLVALAHSMITSKPACMLDSRETQQISSLITQAFLYSIPCNSSGTACFGRTVTIGIEGQSVNHSSVFPGLLLDVPEMLLPGDLERLGDGPFKVVLFGVSLSGDISEVGDVALEVHRGLNPERDLLQQLLKIGEQVVKDKVSLFACQKVVHPVLQHYLREHEVVVIERLGLALMEPFAKITGARAVASLFSLVPMEAYGLVAGLCFQDCGSKKLLQLLSSKHAAISTMVLCHRNETMLEELKVCAMTCQRAEHVLRLTLREPYALLGGGCTETQLATHITHMNQSTAPTTAAALGISHSEFLMAVESFRSSLLAVALSLEHDGQDCLIDLTYGHRWVMDISSQTEVKHTCGCGLLEDRSNLEKTHLNTACQTFSPVFLENNDTQPKVLDSFSAKLNALNVAVEMASLVLDVKYIIKDVN

Target ORF information:

RefSeq Version	XM_009307468.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307468.2

ORF Insert Sequence:

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGGTT TGTGCAATGA CTTGCCAGAG AGCAGAACAT 
GTGTTGCGAC TGACTCTGAG GGAGCCCTAC GCCCTGCTTG GAGGCGGCTG CACAGAAACA 
CAGCTGGCCA CCCACATCAC ACATATGAAT CAGTCCACAG CTCCAACCAC TGCTGCAGCT 
CTGGGTATTT CCCATTCAGA GTTCCTGATG GCAGTCGAGT CATTTCGCAG TTCTTTACTT 
GCTGTGGCTC TTTCCTTAGA GCATGATGGA CAGGACTGTC TCATTGACCT GACATATGGC 
CACAGATGGG TCATGGATAT CAGTTCTCAG ACAGAAGTTA AGCACACCTG TGGTTGTGGA 
CTGCTGGAGG ACAGATCCAA CCTGGAGAAG ACTCATCTAA ATACAGCTTG TCAGACTTTT 
TCTCCTGTTT TCCTAGAAAA CAATGACACT CAGCCTAAAG TATTAGATTC CTTTTCTGCC 
AAGCTTAATG CGTTGAATGT GGCAGTTGAG ATGGCTAGTT TGGTTCTTGA TGTAAAATAT 
ATAATCAAGG ATGTTAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkks ( XM_021480540.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336215.1 Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40376
Clone ID Related Accession (Same CDS sequence)	XM_009307468.2 , XM_021480540.1
Accession Version	XM_021480540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGGTT TGTGCAATGA CTTGCCAGAG AGCAGAACAT 
GTGTTGCGAC TGACTCTGAG GGAGCCCTAC GCCCTGCTTG GAGGCGGCTG CACAGAAACA 
CAGCTGGCCA CCCACATCAC ACATATGAAT CAGTCCACAG CTCCAACCAC TGCTGCAGCT 
CTGGGTATTT CCCATTCAGA GTTCCTGATG GCAGTCGAGT CATTTCGCAG TTCTTTACTT 
GCTGTGGCTC TTTCCTTAGA GCATGATGGA CAGGACTGTC TCATTGACCT GACATATGGC 
CACAGATGGG TCATGGATAT CAGTTCTCAG ACAGAAGTTA AGCACACCTG TGGTTGTGGA 
CTGCTGGAGG ACAGATCCAA CCTGGAGAAG ACTCATCTAA ATACAGCTTG TCAGACTTTT 
TCTCCTGTTT TCCTAGAAAA CAATGACACT CAGCCTAAAG TATTAGATTC CTTTTCTGCC 
AAGCTTAATG CGTTGAATGT GGCAGTTGAG ATGGCTAGTT TGGTTCTTGA TGTAAAATAT 
ATAATCAAGG ATGTTAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336215.1
CDS	201..1901
Translation	MSRISKKKPALCTDEPLSNSTICQKITLLRNILSTAYGPTGRLKQIHNNVGGHVLTTSTSTALLKRLEMSEPLLKLISTALQHHTTRYSDSGLFMGIFTLTLIENTKKYGLRTSTAIKVYKHLVEQCNVYLKGDSCGCKVPVEFSSCDSLVALAHSMITSKPACMLDSRETQQISSLITQAFLYSIPCNSSGTACFGRTVTIGIEGQSVNHSSVFPGLLLDVPEMLLPGDLERLGDGPFKVVLFGVSLSGDISEVGDVALEVHRGLNPERDLLQQLLKIGEQVVKDKVSLFACQKVVHPVLQHYLREHEVVVIERLGLALMEPFAKITGARAVASLFSLVPMEAYGLVAGLCFQDCGSKKLLQLLSSKHAAISTMVLCHRNETMLEELKVCAMTCQRAEHVLRLTLREPYALLGGGCTETQLATHITHMNQSTAPTTAAALGISHSEFLMAVESFRSSLLAVALSLEHDGQDCLIDLTYGHRWVMDISSQTEVKHTCGCGLLEDRSNLEKTHLNTACQTFSPVFLENNDTQPKVLDSFSAKLNALNVAVEMASLVLDVKYIIKDVN

Target ORF information:

RefSeq Version	XM_021480540.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480540.1

ORF Insert Sequence:

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGGTT TGTGCAATGA CTTGCCAGAG AGCAGAACAT 
GTGTTGCGAC TGACTCTGAG GGAGCCCTAC GCCCTGCTTG GAGGCGGCTG CACAGAAACA 
CAGCTGGCCA CCCACATCAC ACATATGAAT CAGTCCACAG CTCCAACCAC TGCTGCAGCT 
CTGGGTATTT CCCATTCAGA GTTCCTGATG GCAGTCGAGT CATTTCGCAG TTCTTTACTT 
GCTGTGGCTC TTTCCTTAGA GCATGATGGA CAGGACTGTC TCATTGACCT GACATATGGC 
CACAGATGGG TCATGGATAT CAGTTCTCAG ACAGAAGTTA AGCACACCTG TGGTTGTGGA 
CTGCTGGAGG ACAGATCCAA CCTGGAGAAG ACTCATCTAA ATACAGCTTG TCAGACTTTT 
TCTCCTGTTT TCCTAGAAAA CAATGACACT CAGCCTAAAG TATTAGATTC CTTTTCTGCC 
AAGCTTAATG CGTTGAATGT GGCAGTTGAG ATGGCTAGTT TGGTTCTTGA TGTAAAATAT 
ATAATCAAGG ATGTTAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkks ( XM_021480541.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336216.1 Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X2, mRNA.

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CloneID	ODa148495
Clone ID Related Accession (Same CDS sequence)	XM_021480541.1
Accession Version	XM_021480541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGATG ACTTGCCAGA GAGCAGAACA TGTGTTGCGA 
CTGACTCTGA GGGAGCCCTA CGCCCTGCTT GGAGGCGGCT GCACAGAAAC ACAGCTGGCC 
ACCCACATCA CACATATGAA TCAGTCCACA GCTCCAACCA CTGCTGCAGC TCTGGGTATT 
TCCCATTCAG AGTTCCTGAT GGCAGTCGAG TCATTTCGCA GTTCTTTACT TGCTGTGGCT 
CTTTCCTTAG AGCATGATGG ACAGGACTGT CTCATTGACC TGACATATGG CCACAGATGG 
GTCATGGATA TCAGTTCTCA GACAGAAGTT AAGCACACCT GTGGTTGTGG ACTGCTGGAG 
GACAGATCCA ACCTGGAGAA GACTCATCTA AATACAGCTT GTCAGACTTT TTCTCCTGTT 
TTCCTAGAAA ACAATGACAC TCAGCCTAAA GTATTAGATT CCTTTTCTGC CAAGCTTAAT 
GCGTTGAATG TGGCAGTTGA GATGGCTAGT TTGGTTCTTG ATGTAAAATA TATAATCAAG 
GATGTTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336216.1
CDS	201..1892
Translation	MSRISKKKPALCTDEPLSNSTICQKITLLRNILSTAYGPTGRLKQIHNNVGGHVLTTSTSTALLKRLEMSEPLLKLISTALQHHTTRYSDSGLFMGIFTLTLIENTKKYGLRTSTAIKVYKHLVEQCNVYLKGDSCGCKVPVEFSSCDSLVALAHSMITSKPACMLDSRETQQISSLITQAFLYSIPCNSSGTACFGRTVTIGIEGQSVNHSSVFPGLLLDVPEMLLPGDLERLGDGPFKVVLFGVSLSGDISEVGDVALEVHRGLNPERDLLQQLLKIGEQVVKDKVSLFACQKVVHPVLQHYLREHEVVVIERLGLALMEPFAKITGARAVASLFSLVPMEAYGLVAGLCFQDCGSKKLLQLLSSKHAAISTMVLCHRNETMLEELKMTCQRAEHVLRLTLREPYALLGGGCTETQLATHITHMNQSTAPTTAAALGISHSEFLMAVESFRSSLLAVALSLEHDGQDCLIDLTYGHRWVMDISSQTEVKHTCGCGLLEDRSNLEKTHLNTACQTFSPVFLENNDTQPKVLDSFSAKLNALNVAVEMASLVLDVKYIIKDVN

Target ORF information:

RefSeq Version	XM_021480541.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio McKusick-Kaufman syndrome (mkks), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480541.1

ORF Insert Sequence:

ATGTCTCGCA TTAGTAAGAA GAAGCCAGCA TTATGCACAG ACGAACCTCT TTCCAACAGT 
ACCATCTGCC AAAAGATCAC TCTATTAAGA AACATACTCA GCACAGCCTA TGGACCTACC 
GGCAGACTGA AACAAATACA CAACAACGTT GGGGGCCACG TCTTGACAAC TTCAACCTCA 
ACAGCGCTTC TCAAACGACT AGAAATGTCT GAGCCGCTTC TCAAACTGAT CTCAACTGCT 
CTTCAACATC ACACCACACG TTACAGTGAC TCAGGGCTGT TTATGGGGAT TTTTACACTC 
ACACTTATTG AAAACACCAA AAAATATGGG CTTAGGACAT CCACGGCAAT CAAAGTGTAC 
AAACACCTTG TGGAACAATG TAATGTGTAT CTTAAAGGAG ACTCTTGTGG CTGTAAGGTG 
CCAGTGGAGT TTAGCAGCTG TGATTCTCTT GTTGCATTGG CGCACAGCAT GATCACCAGC 
AAACCAGCCT GCATGTTGGA CAGCAGAGAG ACGCAGCAGA TCAGTTCATT GATCACACAG 
GCTTTTCTGT ACTCCATCCC TTGCAACTCT TCTGGTACAG CCTGCTTTGG AAGAACGGTC 
ACTATTGGCA TTGAGGGTCA ATCTGTGAAC CATTCTTCAG TTTTTCCTGG ACTGCTGCTG 
GATGTTCCTG AGATGCTCCT GCCTGGAGAT CTCGAAAGAC TGGGAGATGG TCCGTTTAAG 
GTGGTGCTTT TTGGTGTGTC ACTGTCGGGG GATATTTCTG AGGTTGGCGA TGTTGCTTTG 
GAGGTCCACA GAGGATTGAA CCCCGAACGA GATCTCCTTC AGCAGCTCTT GAAGATTGGG 
GAACAGGTTG TAAAGGATAA AGTGAGTTTA TTCGCATGCC AAAAAGTGGT ACATCCAGTT 
CTTCAGCACT ACCTGAGAGA GCATGAAGTG GTCGTGATCG AAAGGCTCGG ACTCGCTCTG 
ATGGAGCCGT TTGCCAAGAT AACAGGTGCA CGGGCTGTTG CATCTCTTTT CTCTCTGGTC 
CCGATGGAAG CTTATGGGTT GGTTGCTGGA TTATGTTTCC AGGACTGTGG ATCCAAGAAG 
CTGCTTCAGC TCCTCTCCTC TAAACATGCT GCAATAAGCA CTATGGTCCT CTGCCACAGA 
AATGAAACTA TGCTGGAAGA ACTGAAGATG ACTTGCCAGA GAGCAGAACA TGTGTTGCGA 
CTGACTCTGA GGGAGCCCTA CGCCCTGCTT GGAGGCGGCT GCACAGAAAC ACAGCTGGCC 
ACCCACATCA CACATATGAA TCAGTCCACA GCTCCAACCA CTGCTGCAGC TCTGGGTATT 
TCCCATTCAG AGTTCCTGAT GGCAGTCGAG TCATTTCGCA GTTCTTTACT TGCTGTGGCT 
CTTTCCTTAG AGCATGATGG ACAGGACTGT CTCATTGACC TGACATATGG CCACAGATGG 
GTCATGGATA TCAGTTCTCA GACAGAAGTT AAGCACACCT GTGGTTGTGG ACTGCTGGAG 
GACAGATCCA ACCTGGAGAA GACTCATCTA AATACAGCTT GTCAGACTTT TTCTCCTGTT 
TTCCTAGAAA ACAATGACAC TCAGCCTAAA GTATTAGATT CCTTTTCTGC CAAGCTTAAT 
GCGTTGAATG TGGCAGTTGA GATGGCTAGT TTGGTTCTTG ATGTAAAATA TATAATCAAG 
GATGTTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein.
PLoS genetics13(7)e1006936(2017 Jul)
Scott CA,Marsden AN,Rebagliati MR,Zhang Q,Chamling X,Searby CC,Baye LM,Sheffield VC,Slusarski DC

Combining Cep290 and Mkks ciliopathy alleles in mice rescues sensory defects and restores ciliogenesis.
The Journal of clinical investigation122(4)1233-45(2012 Apr)
Rachel RA,May-Simera HL,Veleri S,Gotoh N,Choi BY,Murga-Zamalloa C,McIntyre JC,Marek J,Lopez I,Hackett AN,Zhang J,Brooks M,den Hollander AI,Beales PL,Li T,Jacobson SG,Sood R,Martens JR,Liu P,Friedman TB,Khanna H,Koenekoop RK,Kelley MW,Swaroop A

TTC21B contributes both causal and modifying alleles across the ciliopathy spectrum.
Nature genetics43(3)189-96(2011 Mar)
Davis EE,Zhang Q,Liu Q,Diplas BH,Davey LM,Hartley J,Stoetzel C,Szymanska K,Ramaswami G,Logan CV,Muzny DM,Young AC,Wheeler DA,Cruz P,Morgan M,Lewis LR,Cherukuri P,Maskeri B,Hansen NF,Mullikin JC,Blakesley RW,Bouffard GG,,Gyapay G,Rieger S,T?nshoff B,Kern I,Soliman NA,Neuhaus TJ,Swoboda KJ,Kayserili H,Gallagher TE,Lewis RA,Bergmann C,Otto EA,Saunier S,Scambler PJ,Beales PL,Gleeson JG,Maher ER,Atti?-Bitach T,Dollfus H,Johnson CA,Green ED,Gibbs RA,Hildebrandt F,Pierce EA,Katsanis N

Functional analyses of variants reveal a significant role for dominant negative and common alleles in oligogenic Bardet-Biedl syndrome.
Proceedings of the National Academy of Sciences of the United States of America107(23)10602-7(2010 Jun)
Zaghloul NA,Liu Y,Gerdes JM,Gascue C,Oh EC,Leitch CC,Bromberg Y,Binkley J,Leibel RL,Sidow A,Badano JL,Katsanis N

Genetic interaction between Bardet-Biedl syndrome genes and implications for limb patterning.
Human molecular genetics17(13)1956-67(2008 Jul)
Tayeh MK,Yen HJ,Beck JS,Searby CC,Westfall TA,Griesbach H,Sheffield VC,Slusarski DC

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mkks cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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