hsd17b4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsd17b4
Entrez Gene ID	393105
Full Name	hydroxysteroid (17-beta) dehydrogenase 4
Synonyms	Dbp,zgc:55545,zgc:77300
General protein information	Preferred Names hydroxysteroid (17-beta) dehydrogenase 4 Names peroxisomal multifunctional enzyme type 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200136.1	NP_956430.1	peroxisomal multifunctional enzyme type 2
XM_021478207.1	XP_021333882.1	peroxisomal multifunctional enzyme type 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD17B4	NP_000405.1
Mus musculus (house mouse)	Hsd17b4	NP_032318.2
Eremothecium gossypii	AGOS_AGL060W	NP_986606.1
Neurospora crassa	NCU08828	XP_962039.1
Arabidopsis thaliana (thale cress)	ECH2	NP_177742.2
Rattus norvegicus (Norway rat)	Hsd17b4	NP_077368.2
Danio rerio (zebrafish)	hsd17b4	NP_956430.1
Oryza sativa (rice)	Os09g0544900	NP_001063833.1
	HSD17B4	XP_001087837.2
Canis lupus familiaris (dog)	HSD17B4	XP_531860.2
Bos taurus (cattle)	HSD17B4	NP_001007810.1
Gallus gallus (chicken)	HSD17B4	NP_990274.1
Drosophila melanogaster (fruit fly)	Mfe2	NP_573109.1
Caenorhabditis elegans (roundworm)	dhs-28	NP_509146.1
Saccharomyces cerevisiae (baker's yeast)	FOX2	NP_012934.1
Kluyveromyces lactis	KLLA0E13817g	XP_454574.1
Xenopus tropicalis (tropical clawed frog)	hsd17b4	NP_001027490.1
Pan troglodytes (chimpanzee)	HSD17B4	XP_003310890.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008667	XP_314766.4
Magnaporthe oryzae (rice blast fungus)	MGG_06148	XP_003712026.1

Gene Ontology
Bibliography

Related articles in PubMed

Paternal exposure to environmental 17-alpha-ethinylestradiol concentrations modifies testicular transcription, affecting the sperm transcript content and the offspring performance in zebrafish.
Valcarce DG, Vuelta E, Robles V, Herr?ez MP
Aquatic toxicology (Amsterdam, Netherlands)19318-29(2017 Dec)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

Developmental roles of D-bifunctional protein-A zebrafish model of peroxisome dysfunction.
Kim YI, Bhandari S, Lee JN, Yoo KW, Kim SJ, Oh GS, Kim HJ, Cho M, Kwak JY, So HS, Park R, Choe SK
Molecules and cells37(1)74-80(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The knockdown of dbp in zebrafish phenocopied clinical manifestations of its deficiency in human.
Title: Developmental roles of D-bifunctional protein-A zebrafish model of peroxisome dysfunction.

The following hsd17b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsd17b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36165	NM_200136.1 Latest version!	Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa143662	XM_021478207.1 Latest version!	Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsd17b4 ( NM_200136.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956430.1 Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), mRNA.

Price & Availability↑

CloneID	ODa36165
Clone ID Related Accession (Same CDS sequence)	NM_200136.1
Accession Version	NM_200136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	peroxisomal multifunctional enzyme type 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044422.1. On Jul 6, 2005 this sequence version replaced XM_697125.1. ##Evidence-Data-START## Transcript exon combination :: BC044422.1, GDQQ01009955.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGTGC CTTTGAGATT CGACGGCAAG GTGGTGCTGG TGACCGGAGC AGGCGGAGGT 
TTGGGCAGGG AATACGCGCT GGCGTTCGGG CAGAGAGGAG CCGCTGTCAT CGTCAATGAT 
CTGGGTGGAG ATATTAAAGG TGGAGGCAAG AGCTCAGCGG CTGCTGATAA AGTGGTGGAG 
GAGATCAGAG CTGCAGGAGG GAAGGCTGTG GCCAACTATG ATTCAGTGGA GGATGGAGAG 
AAGCTTATCC AGACGGCGCT GGATGCGTTC GGAAGAATAG ACGTGGTGGT GAATAACGCT 
GGGATTCTGC GTGATCGCTC ATTCGCGCGC ACCAGTGATG TGGACTGGGA CCTGATTCAG 
CGTGTTCATC TCAGAGGATC GTTCCTCGTC ACCAGAGCCG CCTGGAACCA CATGAAGCAG 
CAGAAGTTCG GCAGGATCAT CATGACCTCG TCCGCCGCAG GGATTTATGG GAACTTCGGG 
CAGGCCAACT ACAGCGCGGC GAAGCTGGGT CTGCTGGGTC TGGCAAACAC ACTCGCCATC 
GAAGGACAGA AGTACAACAT TCACTGCAAC ACCATCGCCC CCACTGCAGG GTCCAGACTC 
ACCGAGACCG TCATGCCTCC AGATCTGGTC CAGTCTCTGA AGGCGGAGTA CGTGGCGCCG 
CTGGTGCTCT GGCTCTGTCA TGAGTCCTGC CAGGAGAACA GCGGCCTGTT TGAGGTCGGC 
GCTGGCTGGA TCGGCAAATT GCGCTGGGAG AGGAGTCTGG GTCGTATCGT CCGTCAGAAG 
AGCGAGTGTG TGACGCCGGA GGCTGTGAGA GACGCCTGGA GAGACATCTG TGACTTCACT 
AATGCTACTA AACCTGCTAG CATACAGGAG TCGCTGCAGA CGCTGGTGGA GGTTTTGTCC 
AGAGTGGAGG ACGAGAGAAA GATCGGAGCA AACCCAACAG CAGTCGCAAC AAACCCTGCG 
CAGGCCATCG GGCATGTGCT GCCGGACATG ACGTTCACAC ACACACACAT GAACTGTATC 
CTGTACGCTC TGGGAGTGGG CGTGTCCAGC AGAGACCCTC AGCAGCTGCA GTTCCTCTAT 
GAGGGACACA CACACTTCTC CTGCCTGCCC ACGTTCGGGG TGATCCCGGC GCAGGGGGCG 
CTGCTGGGGC TGGGGAGCAT CCCGGGACTC GACATCGACT TCACCAGGCT GCTGCATGGA 
GAGCAGTACC TGGAGCTGTA CAAGCCTCTG CCCACATCAG GTACTCTGAC GTCTCGAGCC 
ACAGTGGCGG ATGTGCTGGA TAAAGGCTCC GGGATGCTGA TCCTGCTGGA TGTGCACACG 
TACAGTGAGC AGGAGCTGCT GTGTTATAAC CAGTTCTCGG TGTTCATCGT GGGCTCTGGA 
GGATTCGGAG GGAAGCGAGT GTCCCAGAAG GCCGTGGCTC CAGCCGCTCC TCCAGACAGA 
CCTGCAGACG CTGTGGTGGT GGAGGAGACA TCTAGAGATC AGGCTGCTCT GTACAGGTTG 
AGTGGAGACT GGAACCCGCT GCACATCGAC CCCAACTTCG CTGCTATGGG AGGCTTTCAG 
TCTCCAATCC TCCACGGTTT GTGTTCGTTT GGTTTCGCGG CGCGGCACGT CCTCAAGCAG 
TTCGCAGGAA ACGACGTCTC CAGATTCAAG GCCATGAAGG TGCGTTTCGT GAAGCCGGTC 
TATCCCGGTC AGTCTCTGCA GACGGAGATG TGGAAGGAGA ACAGCAGAGT CCACATCCAG 
TGTACGGTGA AGGAAAGCGG AGCTGTTGTG CTGTCTGGAG CTTATATAGA TCTACACCCT 
GCAGCATCTG TGAACACCGG CCCGCCACAG ACGGAGCTGC AGAGTGATCT GGTGTTCGCT 
GAGATCGAGC GCAGGATTAA AGACTCAGGA GAGGAGCTGG TGAAGAAGGT GAACGCAGTG 
TTCGGCTGGG AGATCACCAC TGACGGAGAG ACCCGCAGAC ACTGGACGGT GGACCTGAAG 
ACGGGTCGGG GCTCAGTGCA GCGGGCCGCA GCTAAAGCAG ACGTCACCTT CACCGTGTCG 
GATCAGGACT TCATGGAGGT GGTGATGGGG AAACTGAACC CACAGAAGGC CTTCTTCGCT 
GGTAAACTGA AAGTGAAGGG GAATATAATG CTGAGCCAGA AGCTGGAGGC CGTGCTGAAG 
GACCAGGCCC GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956430.1
CDS	70..2247
Translation	MSVPLRFDGKVVLVTGAGGGLGREYALAFGQRGAAVIVNDLGGDIKGGGKSSAAADKVVEEIRAAGGKAVANYDSVEDGEKLIQTALDAFGRIDVVVNNAGILRDRSFARTSDVDWDLIQRVHLRGSFLVTRAAWNHMKQQKFGRIIMTSSAAGIYGNFGQANYSAAKLGLLGLANTLAIEGQKYNIHCNTIAPTAGSRLTETVMPPDLVQSLKAEYVAPLVLWLCHESCQENSGLFEVGAGWIGKLRWERSLGRIVRQKSECVTPEAVRDAWRDICDFTNATKPASIQESLQTLVEVLSRVEDERKIGANPTAVATNPAQAIGHVLPDMTFTHTHMNCILYALGVGVSSRDPQQLQFLYEGHTHFSCLPTFGVIPAQGALLGLGSIPGLDIDFTRLLHGEQYLELYKPLPTSGTLTSRATVADVLDKGSGMLILLDVHTYSEQELLCYNQFSVFIVGSGGFGGKRVSQKAVAPAAPPDRPADAVVVEETSRDQAALYRLSGDWNPLHIDPNFAAMGGFQSPILHGLCSFGFAARHVLKQFAGNDVSRFKAMKVRFVKPVYPGQSLQTEMWKENSRVHIQCTVKESGAVVLSGAYIDLHPAASVNTGPPQTELQSDLVFAEIERRIKDSGEELVKKVNAVFGWEITTDGETRRHWTVDLKTGRGSVQRAAAKADVTFTVSDQDFMEVVMGKLNPQKAFFAGKLKVKGNIMLSQKLEAVLKDQARL

Target ORF information:

RefSeq Version	NM_200136.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200136.1

ORF Insert Sequence:

ATGTCTGTGC CTTTGAGATT CGACGGCAAG GTGGTGCTGG TGACCGGAGC AGGCGGAGGT 
TTGGGCAGGG AATACGCGCT GGCGTTCGGG CAGAGAGGAG CCGCTGTCAT CGTCAATGAT 
CTGGGTGGAG ATATTAAAGG TGGAGGCAAG AGCTCAGCGG CTGCTGATAA AGTGGTGGAG 
GAGATCAGAG CTGCAGGAGG GAAGGCTGTG GCCAACTATG ATTCAGTGGA GGATGGAGAG 
AAGCTTATCC AGACGGCGCT GGATGCGTTC GGAAGAATAG ACGTGGTGGT GAATAACGCT 
GGGATTCTGC GTGATCGCTC ATTCGCGCGC ACCAGTGATG TGGACTGGGA CCTGATTCAG 
CGTGTTCATC TCAGAGGATC GTTCCTCGTC ACCAGAGCCG CCTGGAACCA CATGAAGCAG 
CAGAAGTTCG GCAGGATCAT CATGACCTCG TCCGCCGCAG GGATTTATGG GAACTTCGGG 
CAGGCCAACT ACAGCGCGGC GAAGCTGGGT CTGCTGGGTC TGGCAAACAC ACTCGCCATC 
GAAGGACAGA AGTACAACAT TCACTGCAAC ACCATCGCCC CCACTGCAGG GTCCAGACTC 
ACCGAGACCG TCATGCCTCC AGATCTGGTC CAGTCTCTGA AGGCGGAGTA CGTGGCGCCG 
CTGGTGCTCT GGCTCTGTCA TGAGTCCTGC CAGGAGAACA GCGGCCTGTT TGAGGTCGGC 
GCTGGCTGGA TCGGCAAATT GCGCTGGGAG AGGAGTCTGG GTCGTATCGT CCGTCAGAAG 
AGCGAGTGTG TGACGCCGGA GGCTGTGAGA GACGCCTGGA GAGACATCTG TGACTTCACT 
AATGCTACTA AACCTGCTAG CATACAGGAG TCGCTGCAGA CGCTGGTGGA GGTTTTGTCC 
AGAGTGGAGG ACGAGAGAAA GATCGGAGCA AACCCAACAG CAGTCGCAAC AAACCCTGCG 
CAGGCCATCG GGCATGTGCT GCCGGACATG ACGTTCACAC ACACACACAT GAACTGTATC 
CTGTACGCTC TGGGAGTGGG CGTGTCCAGC AGAGACCCTC AGCAGCTGCA GTTCCTCTAT 
GAGGGACACA CACACTTCTC CTGCCTGCCC ACGTTCGGGG TGATCCCGGC GCAGGGGGCG 
CTGCTGGGGC TGGGGAGCAT CCCGGGACTC GACATCGACT TCACCAGGCT GCTGCATGGA 
GAGCAGTACC TGGAGCTGTA CAAGCCTCTG CCCACATCAG GTACTCTGAC GTCTCGAGCC 
ACAGTGGCGG ATGTGCTGGA TAAAGGCTCC GGGATGCTGA TCCTGCTGGA TGTGCACACG 
TACAGTGAGC AGGAGCTGCT GTGTTATAAC CAGTTCTCGG TGTTCATCGT GGGCTCTGGA 
GGATTCGGAG GGAAGCGAGT GTCCCAGAAG GCCGTGGCTC CAGCCGCTCC TCCAGACAGA 
CCTGCAGACG CTGTGGTGGT GGAGGAGACA TCTAGAGATC AGGCTGCTCT GTACAGGTTG 
AGTGGAGACT GGAACCCGCT GCACATCGAC CCCAACTTCG CTGCTATGGG AGGCTTTCAG 
TCTCCAATCC TCCACGGTTT GTGTTCGTTT GGTTTCGCGG CGCGGCACGT CCTCAAGCAG 
TTCGCAGGAA ACGACGTCTC CAGATTCAAG GCCATGAAGG TGCGTTTCGT GAAGCCGGTC 
TATCCCGGTC AGTCTCTGCA GACGGAGATG TGGAAGGAGA ACAGCAGAGT CCACATCCAG 
TGTACGGTGA AGGAAAGCGG AGCTGTTGTG CTGTCTGGAG CTTATATAGA TCTACACCCT 
GCAGCATCTG TGAACACCGG CCCGCCACAG ACGGAGCTGC AGAGTGATCT GGTGTTCGCT 
GAGATCGAGC GCAGGATTAA AGACTCAGGA GAGGAGCTGG TGAAGAAGGT GAACGCAGTG 
TTCGGCTGGG AGATCACCAC TGACGGAGAG ACCCGCAGAC ACTGGACGGT GGACCTGAAG 
ACGGGTCGGG GCTCAGTGCA GCGGGCCGCA GCTAAAGCAG ACGTCACCTT CACCGTGTCG 
GATCAGGACT TCATGGAGGT GGTGATGGGG AAACTGAACC CACAGAAGGC CTTCTTCGCT 
GGTAAACTGA AAGTGAAGGG GAATATAATG CTGAGCCAGA AGCTGGAGGC CGTGCTGAAG 
GACCAGGCCC GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd17b4 ( XM_021478207.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333882.1 Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143662
Clone ID Related Accession (Same CDS sequence)	XM_021478207.1
Accession Version	XM_021478207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	peroxisomal multifunctional enzyme type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGC CTTTGAGATT CGACGGCAAG GTGGTGCTGG TGACCGGAGC AGGCGGAGGT 
TTGGGCAGGG AATACGCGCT GGCGTTCGGG CAGAGAGGAG CCGCTGTCAT CGTCAATGAT 
CTGGGTGGAG ATATTAAAGG TGGAGGCAAG AGCTCAGCGG CTGCTGATAA AGTGGTGGAG 
GAGATCAGAG CTGCAGGAGG GAAGGCTGTG GCCAACTATG ATTCAGTGGA GGATGGAGAG 
AAGCTCATCC AGACGGCGCT GGATGCGTTC GGAAGAATAG ACGTGGTGGT GAATAACGCT 
GGGATTCTGC GTGATCGCTC ATTCGCGCGC ACCAGTGATG TGGACTGGGA CCTGATTCAG 
CGTGTTCATC TCAGAGGATC GTTCCTCGTC ACCAGAGCCG CCTGGAACCA CATGAAGCAG 
CAGAAGTTCG GCAGGATCAT CATGACCTCG TCCGCCGCAG GGATTTATGG GAACTTCGGG 
CAGGCCAACT ACAGCGCGGC GAAGCTGGGT CTGCTGGGTC TGGCAAACAC ACTGGCCATC 
GAAGGACAGA AGTACAACAT TCACTGCAAC ACCATCGCCC CCACTGCAGG GTCCAGACTC 
ACCGAGACCG TCATGCCTCC AGATCTGGTC CAGTCTCTGA AGGCGGAGTA CGTGGCGCCG 
CTGGTGCTCT GGCTCTGTCA TGAGTCCTGC CAGGAGAACA GCGGCCTGTT TGAGGTCGGC 
GCTGGCTGGA TCGGCAAATT GCGCTGGGAG AGGAGTCTGG GTCGTATCGT CCGTCAGAAG 
AGCGAGTGTG TGACGCCGGA GGCTGTGAGA GACGCCTGGA GAGACATCTG TGACTTCACT 
AATGCTACTA AACCTGCTAG CATACAGGAG TCGCTGCAGA CGCTGGTGGA GGTTTTGTCC 
AGAGTGGAGG ACGAGAGAAA GATTGGAGCA AACCCAACAG CAGTCGCAAC AAACCCTGCG 
CAGGCCATCG GGCATGTGCT GCCGGACATG ACGTTCACAC ACACACACAT GAACTGTATC 
CTGTACGCTC TGGGAGTGGG CGTGTCCAGC AGAGACCCTC AGCAGCTGCA GTTCCTCTAT 
GAGGGACACA CACACTTCTC CTGCCTGCCC ACGTTTGGGG TGATCCCGGC GCAGGGGGCG 
CTGCTGGGGC TGGGGAGCAT CCCGGGACTC GACATAGACT TCACCAGGCT GCTGCATGGA 
GAGCAGTACC TGGAGCTGTA CAAGCCTCTG CCCACATCAG GTACTCTGAC GTCTCGAGCC 
ACAGTGGCGG ATGTGCTGGA TAAAGGCTCC GGGATGCTGA TCCTGCTGGA TGTGCACACG 
TACAGTGAGC AGGAGCTGCT GTGTTATAAC CAGTTCTCGG TGTTCATCGT GGGCTCTGGA 
GGATTCGGAG GGAAGCGAGT GTCCCAGAAG GCCGTGGCTC CAGCTGCTCC TCCAGACAGA 
CCTGCAGACG CTGTGGTGGT GGAGGAGACA TCTAGAGATC AGGCTGCTCT GTACAGGCTG 
AGTGGAGACT GGAACCCGCT GCACATCGAC CCCAACTTCG CTGCTATGGG AGGCTTTCAG 
TCTCCAATCC TCCACGGTTT GTGTTCGTTT GGTTTCGCGG CGCGGCACGT CCTCAAGCAG 
TTCGCAGGAA ACGACGTCTC CAGATTCAAG GCCATGAAGG TGCGTTTCGT GAAGCCGGTC 
TATCCTGGTC AGTCTCTGCA GACGGAGATG TGGAAGGAGA ACAGCAGAGT CCACATCCAG 
TGTACAGTGA AGGAAAGCGG AGCTGTTGTG CTGTCTGGAG CTTATATAGA TCTACACCCT 
GCAGCATCTG TGAACACCGG CCCGCCACAG GCACGTACAC ACACACACAC TTACACACAC 
ACACACACAC ACACTGATGC TGAAGGTGTG TTTGCTCCAC AGACGGAGCT GCAGAGTGAT 
CTGGTGTTCG CTGAGATCGA GCGCAGGATT AAAGACTCAG GAGAGGAGCT GGTGAAGAAG 
GTGAACGCAG TGTTCGGCTG GGAGATCACC ACTGACGGAG AGACCCGCAG ACACTGGACG 
GTGGACCTGA AGACGGGTCG GGGCTCAGTG CAGCGGGCCG CAGCTAAAGC AGATGTCACC 
TTCACCGTGT CGGATCAGGA CTTCATGGAG GTGGTGATGG GGAAACTGAA CCCACAGAAG 
GCCTTCTTCG CTGGTAAACT GAAGGTCAAG GGGAATATAA TGCTGAGCCA GAAGCTGGAG 
GCCGTGCTGA AGGACCAGGC CCGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333882.1
CDS	131..2380
Translation	MSVPLRFDGKVVLVTGAGGGLGREYALAFGQRGAAVIVNDLGGDIKGGGKSSAAADKVVEEIRAAGGKAVANYDSVEDGEKLIQTALDAFGRIDVVVNNAGILRDRSFARTSDVDWDLIQRVHLRGSFLVTRAAWNHMKQQKFGRIIMTSSAAGIYGNFGQANYSAAKLGLLGLANTLAIEGQKYNIHCNTIAPTAGSRLTETVMPPDLVQSLKAEYVAPLVLWLCHESCQENSGLFEVGAGWIGKLRWERSLGRIVRQKSECVTPEAVRDAWRDICDFTNATKPASIQESLQTLVEVLSRVEDERKIGANPTAVATNPAQAIGHVLPDMTFTHTHMNCILYALGVGVSSRDPQQLQFLYEGHTHFSCLPTFGVIPAQGALLGLGSIPGLDIDFTRLLHGEQYLELYKPLPTSGTLTSRATVADVLDKGSGMLILLDVHTYSEQELLCYNQFSVFIVGSGGFGGKRVSQKAVAPAAPPDRPADAVVVEETSRDQAALYRLSGDWNPLHIDPNFAAMGGFQSPILHGLCSFGFAARHVLKQFAGNDVSRFKAMKVRFVKPVYPGQSLQTEMWKENSRVHIQCTVKESGAVVLSGAYIDLHPAASVNTGPPQARTHTHTYTHTHTHTDAEGVFAPQTELQSDLVFAEIERRIKDSGEELVKKVNAVFGWEITTDGETRRHWTVDLKTGRGSVQRAAAKADVTFTVSDQDFMEVVMGKLNPQKAFFAGKLKVKGNIMLSQKLEAVLKDQARL

Target ORF information:

RefSeq Version	XM_021478207.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxysteroid (17-beta) dehydrogenase 4 (hsd17b4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478207.1

ORF Insert Sequence:

ATGTCTGTGC CTTTGAGATT CGACGGCAAG GTGGTGCTGG TGACCGGAGC AGGCGGAGGT 
TTGGGCAGGG AATACGCGCT GGCGTTCGGG CAGAGAGGAG CCGCTGTCAT CGTCAATGAT 
CTGGGTGGAG ATATTAAAGG TGGAGGCAAG AGCTCAGCGG CTGCTGATAA AGTGGTGGAG 
GAGATCAGAG CTGCAGGAGG GAAGGCTGTG GCCAACTATG ATTCAGTGGA GGATGGAGAG 
AAGCTCATCC AGACGGCGCT GGATGCGTTC GGAAGAATAG ACGTGGTGGT GAATAACGCT 
GGGATTCTGC GTGATCGCTC ATTCGCGCGC ACCAGTGATG TGGACTGGGA CCTGATTCAG 
CGTGTTCATC TCAGAGGATC GTTCCTCGTC ACCAGAGCCG CCTGGAACCA CATGAAGCAG 
CAGAAGTTCG GCAGGATCAT CATGACCTCG TCCGCCGCAG GGATTTATGG GAACTTCGGG 
CAGGCCAACT ACAGCGCGGC GAAGCTGGGT CTGCTGGGTC TGGCAAACAC ACTGGCCATC 
GAAGGACAGA AGTACAACAT TCACTGCAAC ACCATCGCCC CCACTGCAGG GTCCAGACTC 
ACCGAGACCG TCATGCCTCC AGATCTGGTC CAGTCTCTGA AGGCGGAGTA CGTGGCGCCG 
CTGGTGCTCT GGCTCTGTCA TGAGTCCTGC CAGGAGAACA GCGGCCTGTT TGAGGTCGGC 
GCTGGCTGGA TCGGCAAATT GCGCTGGGAG AGGAGTCTGG GTCGTATCGT CCGTCAGAAG 
AGCGAGTGTG TGACGCCGGA GGCTGTGAGA GACGCCTGGA GAGACATCTG TGACTTCACT 
AATGCTACTA AACCTGCTAG CATACAGGAG TCGCTGCAGA CGCTGGTGGA GGTTTTGTCC 
AGAGTGGAGG ACGAGAGAAA GATTGGAGCA AACCCAACAG CAGTCGCAAC AAACCCTGCG 
CAGGCCATCG GGCATGTGCT GCCGGACATG ACGTTCACAC ACACACACAT GAACTGTATC 
CTGTACGCTC TGGGAGTGGG CGTGTCCAGC AGAGACCCTC AGCAGCTGCA GTTCCTCTAT 
GAGGGACACA CACACTTCTC CTGCCTGCCC ACGTTTGGGG TGATCCCGGC GCAGGGGGCG 
CTGCTGGGGC TGGGGAGCAT CCCGGGACTC GACATAGACT TCACCAGGCT GCTGCATGGA 
GAGCAGTACC TGGAGCTGTA CAAGCCTCTG CCCACATCAG GTACTCTGAC GTCTCGAGCC 
ACAGTGGCGG ATGTGCTGGA TAAAGGCTCC GGGATGCTGA TCCTGCTGGA TGTGCACACG 
TACAGTGAGC AGGAGCTGCT GTGTTATAAC CAGTTCTCGG TGTTCATCGT GGGCTCTGGA 
GGATTCGGAG GGAAGCGAGT GTCCCAGAAG GCCGTGGCTC CAGCTGCTCC TCCAGACAGA 
CCTGCAGACG CTGTGGTGGT GGAGGAGACA TCTAGAGATC AGGCTGCTCT GTACAGGCTG 
AGTGGAGACT GGAACCCGCT GCACATCGAC CCCAACTTCG CTGCTATGGG AGGCTTTCAG 
TCTCCAATCC TCCACGGTTT GTGTTCGTTT GGTTTCGCGG CGCGGCACGT CCTCAAGCAG 
TTCGCAGGAA ACGACGTCTC CAGATTCAAG GCCATGAAGG TGCGTTTCGT GAAGCCGGTC 
TATCCTGGTC AGTCTCTGCA GACGGAGATG TGGAAGGAGA ACAGCAGAGT CCACATCCAG 
TGTACAGTGA AGGAAAGCGG AGCTGTTGTG CTGTCTGGAG CTTATATAGA TCTACACCCT 
GCAGCATCTG TGAACACCGG CCCGCCACAG GCACGTACAC ACACACACAC TTACACACAC 
ACACACACAC ACACTGATGC TGAAGGTGTG TTTGCTCCAC AGACGGAGCT GCAGAGTGAT 
CTGGTGTTCG CTGAGATCGA GCGCAGGATT AAAGACTCAG GAGAGGAGCT GGTGAAGAAG 
GTGAACGCAG TGTTCGGCTG GGAGATCACC ACTGACGGAG AGACCCGCAG ACACTGGACG 
GTGGACCTGA AGACGGGTCG GGGCTCAGTG CAGCGGGCCG CAGCTAAAGC AGATGTCACC 
TTCACCGTGT CGGATCAGGA CTTCATGGAG GTGGTGATGG GGAAACTGAA CCCACAGAAG 
GCCTTCTTCG CTGGTAAACT GAAGGTCAAG GGGAATATAA TGCTGAGCCA GAAGCTGGAG 
GCCGTGCTGA AGGACCAGGC CCGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Paternal exposure to environmental 17-alpha-ethinylestradiol concentrations modifies testicular transcription, affecting the sperm transcript content and the offspring performance in zebrafish.
Aquatic toxicology (Amsterdam, Netherlands)19318-29(2017 Dec)
Valcarce DG,Vuelta E,Robles V,Herr?ez MP

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

Developmental roles of D-bifunctional protein-A zebrafish model of peroxisome dysfunction.
Molecules and cells37(1)74-80(2014 Jan)
Kim YI,Bhandari S,Lee JN,Yoo KW,Kim SJ,Oh GS,Kim HJ,Cho M,Kwak JY,So HS,Park R,Choe SK

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hsd17b4 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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