sp4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sp4
Entrez Gene ID	387291
Full Name	sp4 transcription factor
Synonyms	zgc:64226
General protein information	Preferred Names sp4 transcription factor Names transcription factor Sp4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173735.2	XP_005173792.1	transcription factor Sp4 isoform X1
NM_200124.1	NP_956418.1	transcription factor Sp4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SP4	XP_002707857.3
Mus musculus (house mouse)	Sp4	NP_033265.3
Gallus gallus (chicken)	SP4	XP_418708.2
Xenopus tropicalis (tropical clawed frog)	sp4	XP_002933342.2
Rattus norvegicus (Norway rat)	Sp4	NP_036893.1
Canis lupus familiaris (dog)	SP4	XP_539462.1
Danio rerio (zebrafish)	sp4	NP_956418.1
Homo sapiens (human)	SP4	NP_003103.2
Pan troglodytes (chimpanzee)	SP4	XP_527679.1
	SP4	XP_001101092.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic Editome of Zebrafish under Aminoglycosides Treatment and Its Potential Involvement in Ototoxicity.
Yan S, Lu Y, He L, Zhao X, Wu L, Zhu H, Jiang M, Su Y, Cao W, Tian W, Xing Q
Frontiers in pharmacology8854(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

?-catenin-dependent control of positional information along the AP body axis in planarians involves a teashirt family member.
Reuter H, M?rz M, Vogg MC, Eccles D, Gr?fol-Bold? L, Wehner D, Owlarn S, Adell T, Weidinger G, Bartscherer K
Cell reports10(2)253-65(2015 Jan)

Characterisation, expression and ontogeny of interleukin-6 and its receptors in zebrafish (Danio rerio).
Varela M, Dios S, Novoa B, Figueras A
Developmental and comparative immunology37(1)97-106(2012 May)

Toxicogenomic and phenotypic analyses of bisphenol-A early-life exposure toxicity in zebrafish.
Lam SH, Hlaing MM, Zhang X, Yan C, Duan Z, Zhu L, Ung CY, Mathavan S, Ong CN, Gong Z
PloS one6(12)e28273(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34488	NM_200124.1 Latest version!	Danio rerio sp4 transcription factor (sp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23751	XM_005173735.2 Latest version!	Danio rerio sp4 transcription factor (sp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sp4 ( NM_200124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956418.1 Danio rerio sp4 transcription factor (sp4), mRNA.

Price & Availability↑

CloneID	ODa34488
Clone ID Related Accession (Same CDS sequence)	NM_200124.1
Accession Version	NM_200124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription factor Sp4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053313.1. ##Evidence-Data-START## Transcript exon combination :: BC053313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGATC AAAAGAAGGA AGCCATGGCC ACTGATGGGG GGAAAGCCAG TGGAGGTGAT 
GGGGGTACGA AAGGGAAAAC TTCTGGATCA CAGGATGCAC AGCCGTCTCC TCTTGCATTG 
CTTGCAGCGA CGTGCAGTAA AATCGGAGGT GTTGGCGCTG AGGGTCAGGC GGCTGCACAA 
CAGCAGATAA TAATCGACCC GAACCAAGGT CTGCTTCAGC TGCAGAACCC CACACAGCAG 
CTGGAGCTGG TCCCGGCACA GCTCACCGGA AACGGCTGGC AGATCATCGC CACCTCCCCT 
GCAACCGCAA CCAAGGACAG CATTCAACAG GCTGGCGCTA ATGCAGCGTC CAGCGAAGCC 
GCAGCCGGCC GGAAAGTCAA GGCCGTCGGC TCTAATAATG CAGCGCAGCA GCAGTTTCAG 
ATCATACAGG TGCAGAACCT GCCCAACTCG TCCGGCGGGA TTCAGTATCA GGTCATCCCG 
CATCTTCAAA CCGCTGACGG ACAGCAGATC CAAATAAGCC CCAGCAACCC CGCAGCCTTA 
AGCGTTCAGC CCGAACAGAT CCAGCTCATT TCCACCGGAA ACAACCAAGC CATTCTGGCC 
ACGCCACAGA GGACGGGATC CGCCAGCATC GTCCAAAACC AGACCATCCC GCTTCAGATC 
AGGCCGTCAT TTCCTCTGCA GCTCCAGACC ATTCAGGGTA CGCAAGCACC AATGGTGACT 
ACATTACCCA TAAACCTCGG CGGCGTGACT TTGGCTCTGC CGGTGATCAA TAACGTAGCA 
GGAGCCGGCG GTTCTGTTCA GCTTCTCCAA TCCAGCGATG GCAGCATCTC CAATGGAAAC 
CAGCTGATCA CGACGGTCAC AACAAGCACA GCAGATTCCA CAGGGAGTTC GACCTCCACC 
ACGACCGCCA CAGGAAGCGA TTCGGTCGTC GTGACTTCAG CTGATGGACT ATCTACGACG 
TCCATGGTGT CCGCCGAGAG CCCAAAGGAT AATGAAGCAG ATTTAACCCA ATCCAACGGC 
CTCCAATCCA CATCTGAGCA GGCCGGACAA ATCCAGCAGT TCCAGATCGT GGGCCACCCG 
GTGCTCCAGC AGATCCAGAT CCAGTCTCCG CAGCAGCAGG TGGTGCAGGG CTCCATACAG 
ATCCAGCCCG GGCAGACTTT ACAGCCGGTC CAGCAAAACC TCCAGCTGCA GGCCTTCCAG 
AACCCGGCGC AGGTGCTGAT CCGGACGCCC ACGCTCACGC CGTCGGGGCA GATCAGCTGG 
CAGACGGTGC AGGTGCAGAA CGCAGGCATG CCCCAGCAGC TGACGCTCGC CCCGGTGGCG 
TCCAGCGCAG GCGGTGGGAC GTTTGCTCAG ATTGCTCCGC TGACGCTCGG AGGCTCACCT 
ATCACGCTCA GCGCCGCGCA GCTGCCCGCG GGCTCAGGGG TGCAGACGGT CAACATTGCC 
GGTCTGGGGG CGGCTGGAGT GCAGGTGCAG GGCGTCCCGC TCACCATCAC CGGTGTACAA 
GGTCAACAGC AGGGTCAGGA TGGGGTCAAA GTTCAGTCCG CTCCAGTGAC GGTGACCGTT 
GGGAACATCA GCAATGCTGT GAGTCCTGAG CAGATGGGGC AGGTCCAGAG CCCGTCCGAT 
CAGGAGGGTC AACCCAGCAA GAGGCTGCGC AGGGTGGCCT GTTCCTGCCC CAACTGCAGA 
GATGGAGAGG GCAGAAACAA CAGCGACCCG TCAAAGAAGA AGCAGCATGT GTGTCATATG 
GAGGGTTGCG GGAAGGTTTA TGGGAAAACG TCTCACCTGA GGGCTCACCT GCGCTGGCAC 
ACAGGAGAAC GACCATTCGT CTGTAACTGG ATCTTCTGTG GGAAACGCTT CACGCGGAGC 
GACGAGCTGC AGCGGCACCG CAGGACACAC ACAGGTGAGA AGAGGTTCGA GTGTCCCGAA 
TGCTCCAAGA GGTTCATGCG GAGCGACCAC CTGTCAAAGC ACATCAAAAC CCACCAAAAC 
AAGAAAGGTG GAGCGGCTCT TACCATCATC ACCACAGACG AGATGGAGGA GGAGGTGGAT 
GAGGTGTTGG GCTCGCCGAG GATCGTTACA GTGGCGACGC TCTCACACGA TTCCAACCCG 
GCCACGCCCA CCACTTCAAA CAATTTAGAG GAGGAGTTTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956418.1
CDS	67..2211
Translation	MSDQKKEAMATDGGKASGGDGGTKGKTSGSQDAQPSPLALLAATCSKIGGVGAEGQAAAQQQIIIDPNQGLLQLQNPTQQLELVPAQLTGNGWQIIATSPATATKDSIQQAGANAASSEAAAGRKVKAVGSNNAAQQQFQIIQVQNLPNSSGGIQYQVIPHLQTADGQQIQISPSNPAALSVQPEQIQLISTGNNQAILATPQRTGSASIVQNQTIPLQIRPSFPLQLQTIQGTQAPMVTTLPINLGGVTLALPVINNVAGAGGSVQLLQSSDGSISNGNQLITTVTTSTADSTGSSTSTTTATGSDSVVVTSADGLSTTSMVSAESPKDNEADLTQSNGLQSTSEQAGQIQQFQIVGHPVLQQIQIQSPQQQVVQGSIQIQPGQTLQPVQQNLQLQAFQNPAQVLIRTPTLTPSGQISWQTVQVQNAGMPQQLTLAPVASSAGGGTFAQIAPLTLGGSPITLSAAQLPAGSGVQTVNIAGLGAAGVQVQGVPLTITGVQGQQQGQDGVKVQSAPVTVTVGNISNAVSPEQMGQVQSPSDQEGQPSKRLRRVACSCPNCRDGEGRNNSDPSKKKQHVCHMEGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKGGAALTIITTDEMEEEVDEVLGSPRIVTVATLSHDSNPATPTTSNNLEEEFE

Target ORF information:

RefSeq Version	NM_200124.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sp4 transcription factor (sp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200124.1

ORF Insert Sequence:

ATGAGTGATC AAAAGAAGGA AGCCATGGCC ACTGATGGGG GGAAAGCCAG TGGAGGTGAT 
GGGGGTACGA AAGGGAAAAC TTCTGGATCA CAGGATGCAC AGCCGTCTCC TCTTGCATTG 
CTTGCAGCGA CGTGCAGTAA AATCGGAGGT GTTGGCGCTG AGGGTCAGGC GGCTGCACAA 
CAGCAGATAA TAATCGACCC GAACCAAGGT CTGCTTCAGC TGCAGAACCC CACACAGCAG 
CTGGAGCTGG TCCCGGCACA GCTCACCGGA AACGGCTGGC AGATCATCGC CACCTCCCCT 
GCAACCGCAA CCAAGGACAG CATTCAACAG GCTGGCGCTA ATGCAGCGTC CAGCGAAGCC 
GCAGCCGGCC GGAAAGTCAA GGCCGTCGGC TCTAATAATG CAGCGCAGCA GCAGTTTCAG 
ATCATACAGG TGCAGAACCT GCCCAACTCG TCCGGCGGGA TTCAGTATCA GGTCATCCCG 
CATCTTCAAA CCGCTGACGG ACAGCAGATC CAAATAAGCC CCAGCAACCC CGCAGCCTTA 
AGCGTTCAGC CCGAACAGAT CCAGCTCATT TCCACCGGAA ACAACCAAGC CATTCTGGCC 
ACGCCACAGA GGACGGGATC CGCCAGCATC GTCCAAAACC AGACCATCCC GCTTCAGATC 
AGGCCGTCAT TTCCTCTGCA GCTCCAGACC ATTCAGGGTA CGCAAGCACC AATGGTGACT 
ACATTACCCA TAAACCTCGG CGGCGTGACT TTGGCTCTGC CGGTGATCAA TAACGTAGCA 
GGAGCCGGCG GTTCTGTTCA GCTTCTCCAA TCCAGCGATG GCAGCATCTC CAATGGAAAC 
CAGCTGATCA CGACGGTCAC AACAAGCACA GCAGATTCCA CAGGGAGTTC GACCTCCACC 
ACGACCGCCA CAGGAAGCGA TTCGGTCGTC GTGACTTCAG CTGATGGACT ATCTACGACG 
TCCATGGTGT CCGCCGAGAG CCCAAAGGAT AATGAAGCAG ATTTAACCCA ATCCAACGGC 
CTCCAATCCA CATCTGAGCA GGCCGGACAA ATCCAGCAGT TCCAGATCGT GGGCCACCCG 
GTGCTCCAGC AGATCCAGAT CCAGTCTCCG CAGCAGCAGG TGGTGCAGGG CTCCATACAG 
ATCCAGCCCG GGCAGACTTT ACAGCCGGTC CAGCAAAACC TCCAGCTGCA GGCCTTCCAG 
AACCCGGCGC AGGTGCTGAT CCGGACGCCC ACGCTCACGC CGTCGGGGCA GATCAGCTGG 
CAGACGGTGC AGGTGCAGAA CGCAGGCATG CCCCAGCAGC TGACGCTCGC CCCGGTGGCG 
TCCAGCGCAG GCGGTGGGAC GTTTGCTCAG ATTGCTCCGC TGACGCTCGG AGGCTCACCT 
ATCACGCTCA GCGCCGCGCA GCTGCCCGCG GGCTCAGGGG TGCAGACGGT CAACATTGCC 
GGTCTGGGGG CGGCTGGAGT GCAGGTGCAG GGCGTCCCGC TCACCATCAC CGGTGTACAA 
GGTCAACAGC AGGGTCAGGA TGGGGTCAAA GTTCAGTCCG CTCCAGTGAC GGTGACCGTT 
GGGAACATCA GCAATGCTGT GAGTCCTGAG CAGATGGGGC AGGTCCAGAG CCCGTCCGAT 
CAGGAGGGTC AACCCAGCAA GAGGCTGCGC AGGGTGGCCT GTTCCTGCCC CAACTGCAGA 
GATGGAGAGG GCAGAAACAA CAGCGACCCG TCAAAGAAGA AGCAGCATGT GTGTCATATG 
GAGGGTTGCG GGAAGGTTTA TGGGAAAACG TCTCACCTGA GGGCTCACCT GCGCTGGCAC 
ACAGGAGAAC GACCATTCGT CTGTAACTGG ATCTTCTGTG GGAAACGCTT CACGCGGAGC 
GACGAGCTGC AGCGGCACCG CAGGACACAC ACAGGTGAGA AGAGGTTCGA GTGTCCCGAA 
TGCTCCAAGA GGTTCATGCG GAGCGACCAC CTGTCAAAGC ACATCAAAAC CCACCAAAAC 
AAGAAAGGTG GAGCGGCTCT TACCATCATC ACCACAGACG AGATGGAGGA GGAGGTGGAT 
GAGGTGTTGG GCTCGCCGAG GATCGTTACA GTGGCGACGC TCTCACACGA TTCCAACCCG 
GCCACGCCCA CCACTTCAAA CAATTTAGAG GAGGAGTTTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sp4 ( XM_005173735.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173792.1 Danio rerio sp4 transcription factor (sp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23751
Clone ID Related Accession (Same CDS sequence)	XM_005173735.2
Accession Version	XM_005173735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transcription factor Sp4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005173735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATGGGGGGAA AGCCAGTGGA GGTGATGGGG GTACGAAAGG GAAAACTTCT 
GGATCACAGG ATGCACAGCC GTCTCCTCTT GCATTGCTTG CAGCGACGTG CAGTAAAATC 
GGAGGTGTTG GCGCTGAGGG TCAGGCGGCT GCACAACAGC AGATAATAAT CGACCCGAGC 
CAAGGTCTGC TTCAGCTGCA GAACCCCACA CAGCAGCTGG AGCTGGTCCC GGCACAGCTC 
ACCGGAAACG GCTGGCAGAT CATCGCCACC TCCCCTGCAA CCGCAACCAA GGACAGCATT 
CAACAGGCTG GCGCTAATGC AGCGTCCAGC GAAGCCGCAG CCGGCCGGAA AGTCAAGGCC 
GTCGGCTCTA ATAATGCAGC GCAGCAGCAG TTTCAGATCA TACAGGTGCA GAACCTGCCC 
AACTCGTCCG GCGGGATTCA GTATCAGGTC ATCCCGCATC TTCAAACCGC TGACGGACAG 
CAGATCCAAA TAAGCCCCAG CAACCCCGCA GCCTTAAGCG TTCAGCCCGA ACAGATCCAG 
CTCATTTCCA CCGGAAACAA CCAAGCCATT CTGGCCACGC CACAGAGGAC GGGATCCGCC 
AGCATCGTCC AAAACCAGAC CATCCCGCTT CAGATCAGGC CGTCATTTCC TCTGCAGCTC 
CAGACCATTC AGGGTACGCA AGCACCAATG GTGACTACAT TACCCATAAA CCTCGGCGGC 
GTGACTTTGG CTCTGCCGGT GATCAATAAC GTAGCAGGAG CCGGCGGTTC TGTTCAGCTT 
CTCCAATCCA GCGATGGCAG CATCTCCAAT GGAAACCAGC TGATCACGAC GGTCACAACA 
AGCACAGCAG ATTCCACAGG GAGTTCGACC TCCACCACGA CCGCCACAGG AAGCGATTCG 
GTCGTCGTGA CTTCAGCTGA TGGACTATCT ACGACGTCCA TGGTGTCCGC CGAGAGCCCA 
AAGGATAATG AAGCAGATTT AACCCAATCC AACGGCCTCC AATCCACATC TGAGCAGGCC 
GGACAAATCC AGCAGTTCCA GATCGTGGGC CACCCGGTGC TCCAGCAGAT CCAGATCCAG 
TCTCCGCAGC AGCAGGTGGT GCAGGGCTCC ATACAGATCC AGCCCGGGCA GACTTTACAG 
CCGGTCCAGC AAAACCTCCA GCTGCAGGCC TTCCAGAACC CGGCGCAGGT GCTGATCCGG 
ACGCCCACGC TCACGCCGTC GGGGCAGATC AGCTGGCAGA CGGTGCAGGT GCAGAACGCA 
GGCATGCCCC AGCAGCTGAC GCTCGCCCCG GTGGCGTCCA GCGCAGGCGG TGGGACGTTT 
GCTCAGATTG CTCCGCTGAC GCTCGGAGGC TCACCTATCA CGCTCAGCGC CGCGCAGCTG 
CCCGCGGGCT CAGGGGTGCA GACGGTCAAC ATTGCCGGTC TGGGGGCGGC TGGAGTGCAG 
GTGCAGGGCG TCCCGCTCAC CATCACCGGT GTACAAGGTC AACAGCAGGG TCAGGATGGG 
GTCAAAGTTC AGTCCGCTCC AGTGACGGTG ACCGTTGGGA ACATCAGCAA CGCTGTGAGT 
CCTGAGCAGA TGGGGCAGGT CCAGAGCCCG TCCGATCAGG AGGGTCAACC CAGCAAGAGG 
CTGCGCAGGG TGGCCTGTTC CTGCCCCAAC TGCAGAGATG GAGAGGGCAG AAACAACAGC 
GACCCGTCAA AGAAGAAGCA GCATGTGTGT CATATGGAGG GTTGTGGGAA GGTTTATGGG 
AAAACGTCTC ACCTGAGGGC TCACCTGCGC TGGCACACAG GAGAACGACC ATTCGTCTGT 
AACTGGATCT TCTGTGGGAA ACGCTTCACG CGGAGCGACG AGCTGCAGCG GCACCGCAGA 
ACACACACAG GTGAGAAGAG GTTCGAGTGT CCCGAATGCT CCAAGAGGTT CATGCGGAGC 
GACCACCTGT CAAAGCACAT CAAAACCCAC CAAAACAAGA AAGGTGGAGC AGCTCTTACC 
ATCATCACCA CAGACGAGAT GGAGGAGGAG GTGGATGAGG TGTTGGGCTC GCCGAGGATC 
GTTACAGTGG CGACGCTCTC ACATGATTCC AACCCGGCCA CGCCCACCAC TTCAAACAAT 
TTAGAGGAGG AGTTTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173792.1
CDS	127..2247
Translation	MATDGGKASGGDGGTKGKTSGSQDAQPSPLALLAATCSKIGGVGAEGQAAAQQQIIIDPSQGLLQLQNPTQQLELVPAQLTGNGWQIIATSPATATKDSIQQAGANAASSEAAAGRKVKAVGSNNAAQQQFQIIQVQNLPNSSGGIQYQVIPHLQTADGQQIQISPSNPAALSVQPEQIQLISTGNNQAILATPQRTGSASIVQNQTIPLQIRPSFPLQLQTIQGTQAPMVTTLPINLGGVTLALPVINNVAGAGGSVQLLQSSDGSISNGNQLITTVTTSTADSTGSSTSTTTATGSDSVVVTSADGLSTTSMVSAESPKDNEADLTQSNGLQSTSEQAGQIQQFQIVGHPVLQQIQIQSPQQQVVQGSIQIQPGQTLQPVQQNLQLQAFQNPAQVLIRTPTLTPSGQISWQTVQVQNAGMPQQLTLAPVASSAGGGTFAQIAPLTLGGSPITLSAAQLPAGSGVQTVNIAGLGAAGVQVQGVPLTITGVQGQQQGQDGVKVQSAPVTVTVGNISNAVSPEQMGQVQSPSDQEGQPSKRLRRVACSCPNCRDGEGRNNSDPSKKKQHVCHMEGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKGGAALTIITTDEMEEEVDEVLGSPRIVTVATLSHDSNPATPTTSNNLEEEFE

Target ORF information:

RefSeq Version	XM_005173735.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sp4 transcription factor (sp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173735.2

ORF Insert Sequence:

ATGGCCACTG ATGGGGGGAA AGCCAGTGGA GGTGATGGGG GTACGAAAGG GAAAACTTCT 
GGATCACAGG ATGCACAGCC GTCTCCTCTT GCATTGCTTG CAGCGACGTG CAGTAAAATC 
GGAGGTGTTG GCGCTGAGGG TCAGGCGGCT GCACAACAGC AGATAATAAT CGACCCGAGC 
CAAGGTCTGC TTCAGCTGCA GAACCCCACA CAGCAGCTGG AGCTGGTCCC GGCACAGCTC 
ACCGGAAACG GCTGGCAGAT CATCGCCACC TCCCCTGCAA CCGCAACCAA GGACAGCATT 
CAACAGGCTG GCGCTAATGC AGCGTCCAGC GAAGCCGCAG CCGGCCGGAA AGTCAAGGCC 
GTCGGCTCTA ATAATGCAGC GCAGCAGCAG TTTCAGATCA TACAGGTGCA GAACCTGCCC 
AACTCGTCCG GCGGGATTCA GTATCAGGTC ATCCCGCATC TTCAAACCGC TGACGGACAG 
CAGATCCAAA TAAGCCCCAG CAACCCCGCA GCCTTAAGCG TTCAGCCCGA ACAGATCCAG 
CTCATTTCCA CCGGAAACAA CCAAGCCATT CTGGCCACGC CACAGAGGAC GGGATCCGCC 
AGCATCGTCC AAAACCAGAC CATCCCGCTT CAGATCAGGC CGTCATTTCC TCTGCAGCTC 
CAGACCATTC AGGGTACGCA AGCACCAATG GTGACTACAT TACCCATAAA CCTCGGCGGC 
GTGACTTTGG CTCTGCCGGT GATCAATAAC GTAGCAGGAG CCGGCGGTTC TGTTCAGCTT 
CTCCAATCCA GCGATGGCAG CATCTCCAAT GGAAACCAGC TGATCACGAC GGTCACAACA 
AGCACAGCAG ATTCCACAGG GAGTTCGACC TCCACCACGA CCGCCACAGG AAGCGATTCG 
GTCGTCGTGA CTTCAGCTGA TGGACTATCT ACGACGTCCA TGGTGTCCGC CGAGAGCCCA 
AAGGATAATG AAGCAGATTT AACCCAATCC AACGGCCTCC AATCCACATC TGAGCAGGCC 
GGACAAATCC AGCAGTTCCA GATCGTGGGC CACCCGGTGC TCCAGCAGAT CCAGATCCAG 
TCTCCGCAGC AGCAGGTGGT GCAGGGCTCC ATACAGATCC AGCCCGGGCA GACTTTACAG 
CCGGTCCAGC AAAACCTCCA GCTGCAGGCC TTCCAGAACC CGGCGCAGGT GCTGATCCGG 
ACGCCCACGC TCACGCCGTC GGGGCAGATC AGCTGGCAGA CGGTGCAGGT GCAGAACGCA 
GGCATGCCCC AGCAGCTGAC GCTCGCCCCG GTGGCGTCCA GCGCAGGCGG TGGGACGTTT 
GCTCAGATTG CTCCGCTGAC GCTCGGAGGC TCACCTATCA CGCTCAGCGC CGCGCAGCTG 
CCCGCGGGCT CAGGGGTGCA GACGGTCAAC ATTGCCGGTC TGGGGGCGGC TGGAGTGCAG 
GTGCAGGGCG TCCCGCTCAC CATCACCGGT GTACAAGGTC AACAGCAGGG TCAGGATGGG 
GTCAAAGTTC AGTCCGCTCC AGTGACGGTG ACCGTTGGGA ACATCAGCAA CGCTGTGAGT 
CCTGAGCAGA TGGGGCAGGT CCAGAGCCCG TCCGATCAGG AGGGTCAACC CAGCAAGAGG 
CTGCGCAGGG TGGCCTGTTC CTGCCCCAAC TGCAGAGATG GAGAGGGCAG AAACAACAGC 
GACCCGTCAA AGAAGAAGCA GCATGTGTGT CATATGGAGG GTTGTGGGAA GGTTTATGGG 
AAAACGTCTC ACCTGAGGGC TCACCTGCGC TGGCACACAG GAGAACGACC ATTCGTCTGT 
AACTGGATCT TCTGTGGGAA ACGCTTCACG CGGAGCGACG AGCTGCAGCG GCACCGCAGA 
ACACACACAG GTGAGAAGAG GTTCGAGTGT CCCGAATGCT CCAAGAGGTT CATGCGGAGC 
GACCACCTGT CAAAGCACAT CAAAACCCAC CAAAACAAGA AAGGTGGAGC AGCTCTTACC 
ATCATCACCA CAGACGAGAT GGAGGAGGAG GTGGATGAGG TGTTGGGCTC GCCGAGGATC 
GTTACAGTGG CGACGCTCTC ACATGATTCC AACCCGGCCA CGCCCACCAC TTCAAACAAT 
TTAGAGGAGG AGTTTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic Editome of Zebrafish under Aminoglycosides Treatment and Its Potential Involvement in Ototoxicity.
Frontiers in pharmacology8854(2017)
Yan S,Lu Y,He L,Zhao X,Wu L,Zhu H,Jiang M,Su Y,Cao W,Tian W,Xing Q

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

?-catenin-dependent control of positional information along the AP body axis in planarians involves a teashirt family member.
Cell reports10(2)253-65(2015 Jan)
Reuter H,M?rz M,Vogg MC,Eccles D,Gr?fol-Bold? L,Wehner D,Owlarn S,Adell T,Weidinger G,Bartscherer K

Characterisation, expression and ontogeny of interleukin-6 and its receptors in zebrafish (Danio rerio).
Developmental and comparative immunology37(1)97-106(2012 May)
Varela M,Dios S,Novoa B,Figueras A

Toxicogenomic and phenotypic analyses of bisphenol-A early-life exposure toxicity in zebrafish.
PloS one6(12)e28273(2011)
Lam SH,Hlaing MM,Zhang X,Yan C,Duan Z,Zhu L,Ung CY,Mathavan S,Ong CN,Gong Z

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sp4 cDNA ORF clone, Danio rerio(zebrafish)

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