poc1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	poc1b
Entrez Gene ID	386932
Full Name	POC1 centriolar protein B
Synonyms	TUWD12,wdr51b,wu:fl36w17,zgc:63538
General protein information	Preferred Names POC1 centriolar protein B Names POC1 centriolar protein homolog B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297892.2	XP_009296167.1	POC1 centriolar protein homolog B isoform X1
XM_017354060.2	XP_017209549.1	POC1 centriolar protein homolog B isoform X2
XM_017354060.1	XP_017209549.1	POC1 centriolar protein homolog B isoform X2
XM_009297892.3	XP_009296167.1	POC1 centriolar protein homolog B isoform X1
NM_200118.1	NP_956412.1	POC1 centriolar protein homolog B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POC1B	XP_003952312.1
Homo sapiens (human)	POC1B	NP_758440.1
Xenopus tropicalis (tropical clawed frog)	poc1b	NP_001004988.1
	POC1B	XP_001101566.2
Canis lupus familiaris (dog)	POC1B	XP_005629272.1
Gallus gallus (chicken)	WDR51B	NP_001006232.1
Danio rerio (zebrafish)	poc1b	NP_956412.1
Mus musculus (house mouse)	Poc1b	NP_082016.1
Rattus norvegicus (Norway rat)	Poc1b	XP_002729827.2
Bos taurus (cattle)	POC1B	NP_001092501.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Knockdown of poc1b causes abnormal photoreceptor sensory cilium and vision impairment in zebrafish.
Zhang C, Zhang Q, Wang F, Liu Q
Biochemical and biophysical research communications465(4)651-7(2015 Oct)

Mutation of POC1B in a severe syndromic retinal ciliopathy.
Beck BB, Phillips JB, Bartram MP, Wegner J, Thoenes M, Pannes A, Sampson J, Heller R, G?bel H, Koerber F, Neugebauer A, Hedergott A, N?rnberg G, N?rnberg P, Thiele H, Altm?ller J, Toliat MR, Staubach S, Boycott KM, Valente EM, Janecke AR, Eisenberger T, Bergmann C, Tebbe L, Wang Y, Wu Y, Fry AM, Westerfield M, Wolfrum U, Bolz HJ
Human mutation35(10)1153-62(2014 Oct)

Disruption of the basal body protein POC1B results in autosomal-recessive cone-rod dystrophy.
Roosing S, Lamers IJ, de Vrieze E, van den Born LI, Lambertus S, Arts HH, , Peters TA, Hoyng CB, Kremer H, Hetterschijt L, Letteboer SJ, van Wijk E, Roepman R, den Hollander AI, Cremers FP
American journal of human genetics95(2)131-42(2014 Aug)

A highly conserved Poc1 protein characterized in embryos of the hydrozoan Clytia hemisphaerica: localization and functional studies.
Fourrage C, Chevalier S, Houliston E
PloS one5(11)e13994(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest that poc1b is required for normal development and ciliogenesis of retinal photoreceptor sensory cilia and other cilia
Title: Knockdown of poc1b causes abnormal photoreceptor sensory cilium and vision impairment in zebrafish.

Poc1B knockdown causes ciliary defects & morphological phenotype consistent with ciliopathies; Poc1 provides link between assembly & stability of centrioles for ciliary-based motility in Tetrahymena & cilia formation & function in D rerio & humans
Title: Basal body stability and ciliogenesis requires the conserved component Poc1.

The following poc1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the poc1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02947	NM_200118.1 Latest version!	Danio rerio POC1 centriolar protein B (poc1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa40355	XM_009297892.3 Latest version!	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa57787	XM_017354060.2 Latest version!	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa57787	XM_017354060.1	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa40355	XM_009297892.2	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

poc1b ( NM_200118.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956412.1 Danio rerio POC1 centriolar protein B (poc1b), mRNA.

Price & Availability↑

CloneID	ODa02947
Clone ID Related Accession (Same CDS sequence)	NM_200118.1
Accession Version	NM_200118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Danio rerio(zebrafish)
Product	POC1 centriolar protein homolog B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055275.1. On Jul 6, 2005 this sequence version replaced XM_697112.1. ##Evidence-Data-START## Transcript exon combination :: BC055275.1, AY422998.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTTAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTAATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGACACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCACCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TTTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAGACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGAT GCAAGAAACT ATGACGGTGC ATCAAGCAGC 
AGAGCTCAGT TCACCTCAGG AATGGACTTA GGTCCTCTTC GGACTCATAC ACAAGCCAGA 
GAAGAAGAGG ATGAAAACCA AGAGGAAAGA TTTGCTGGCG GCATGACAGC ATCTCCAGCA 
GAGCGCTCTG GTATTCCATC AAGCCTTACT AGCACTCTGG AGAACATTGT GCAGCAGCTG 
GACATCCTGA CCCAGACCGT TGCTGTCCTC GAGGAGCGTC TTACCCTGAC CGAAGACAAA 
CTGAGGACCT GCCTGGACAA TCAGGTCCTG CTTATGCAAC AAAACCAACA ATTAGACCGA 
TCTGACGAGG AATCCGAGGG ACCCCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956412.1
CDS	123..1595
Translation	MASVMEDPTLERHFKGHKDVISCADFNPNNKQLATGSCDKSLMIWNLAPKARAFRFVGHTDVITGVNFAPSGSLVASSSRDQTVRLWTPSIKGESTVFKAHTASVRSVNFSRDGQRLVTASDDKSVKVWGVERKKFLYSLNRHTNWVRCARFSPDGRLIASCGDDRTVRLWDTSSHQCTNIFTDYGGSATFVDFNSSGTCIASSGADNTIKIWDIRTNKLIQHYKVHNAGVNCFSFHPSGNYLISGSSDSTIKILDLLEGRLIYTLHGHKGPVLTVTFSRDGDLFASGGADSQVLMWKTNFDSLNYRELLSRHSKRVTPDPPPHLMDIYPRSHHRHHPQNGTVEINPVADTQSTDPQVVEVGRVVFSTTDARNYDGASSSRAQFTSGMDLGPLRTHTQAREEEDENQEERFAGGMTASPAERSGIPSSLTSTLENIVQQLDILTQTVAVLEERLTLTEDKLRTCLDNQVLLMQQNQQLDRSDEESEGPPL

Target ORF information:

RefSeq Version	NM_200118.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POC1 centriolar protein B (poc1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200118.1

ORF Insert Sequence:

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTTAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTAATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGACACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCACCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TTTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAGACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGAT GCAAGAAACT ATGACGGTGC ATCAAGCAGC 
AGAGCTCAGT TCACCTCAGG AATGGACTTA GGTCCTCTTC GGACTCATAC ACAAGCCAGA 
GAAGAAGAGG ATGAAAACCA AGAGGAAAGA TTTGCTGGCG GCATGACAGC ATCTCCAGCA 
GAGCGCTCTG GTATTCCATC AAGCCTTACT AGCACTCTGG AGAACATTGT GCAGCAGCTG 
GACATCCTGA CCCAGACCGT TGCTGTCCTC GAGGAGCGTC TTACCCTGAC CGAAGACAAA 
CTGAGGACCT GCCTGGACAA TCAGGTCCTG CTTATGCAAC AAAACCAACA ATTAGACCGA 
TCTGACGAGG AATCCGAGGG ACCCCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poc1b ( XM_009297892.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296167.1 Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40355
Clone ID Related Accession (Same CDS sequence)	XM_009297892.2 , XM_009297892.3
Accession Version	XM_009297892.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	POC1 centriolar protein homolog B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC CCACCCTTGA GAGGCATTTT AAAGGTCATA AAGATGTAAT TTCCTGCGCT 
GACTTCAACC CCAATAATAA ACAGCTTGCT ACTGGATCCT GTGATAAAAG CCTTATGATT 
TGGAACCTCG CTCCTAAAGC CCGGGCTTTC AGGTTTGTGG GTCACACAGA TGTCATTACA 
GGCGTTAATT TTGCTCCAAG CGGTTCATTG GTGGCATCTT CATCCAGAGA TCAAACAGTT 
CGATTGTGGA CACCCAGTAT AAAGGGTGAA TCGACTGTGT TCAAAGCCCA CACAGCAAGC 
GTTCGAAGCG TTCATTTCTC CAGAGATGGC CAAAGGCTGG TCACGGCCTC AGATGACAAG 
TCTGTGAAAG TATGGGGTGT TGAACGGAAA AAATTCCTCT ACTCTCTCAA CCGGCACACC 
AACTGGGTGC GCTGTGCGAG GTTTTCACCA GATGGTCGTC TCATTGCTTC CTGTGGTGAC 
GACAGGACGG TTCGCCTCTG GGATACATCC TCACATCAGT GCATCAACAT TTTCACAGAC 
TATGGAGGGT CTGCCACATT TGTAGACTTC AACTCCAGTG GCACGTGCAT TGCATCATCA 
GGTGCAGACA ACACAATAAA AATCTGGGAT ATTCGTACAA ACAAGTTAAT ACAACATTAC 
AAAGTCCATA ATGCAGGAGT CAACTGCTTT TCCTTCCATC CCTCTGGAAA CTATCTGATC 
AGTGGTTCGA GTGACAGTAC AATTAAGATC TTGGACCTGC TGGAGGGGAG GCTCATCTAC 
ACTCTTCATG GCCATAAGGG TCCAGTTTTG ACCGTGACGT TTTCTCGAGA TGGAGATCTG 
TTTGCATCAG GAGGTGCAGA CTCTCAGGTT CTGATGTGGA AGACCAACTT TGATTCGCTG 
AACTACAGAG AACTGTTGAG CAAACACAGC AAACGCGTGA CTCCAGATCC TCCTCCACAC 
CTGATGGACA TTTACCCTCG ATCACATCAC CGACACCACC CTCAGAACGG CACAGTAGAG 
ATTAATCCTG TTGCTGATAC ACAGTCTACT GACCCTCAGG TGGTTGAAGT GGGACGTGTT 
GTTTTCTCCA CAACGGATGC AAGAAACTAT GATGGTGCAT CAAGCAGCAG AGCTCAGTTC 
ACCTCAGGAA TGGACTCAGG TCCATTTCGG ACTCATACAC AAGCCAGAGA AGAAGAGGAT 
GAAAACCAAG AGGAAAGATT TGCTGGCGGC ATGACAGCAT CTCCAGCAGA GCGCTCTGGT 
ATTCCATCAA GCCTTACTAG CACTCTGGAG AACATTGTAC AGCAGCTGGA CATCCTGACC 
CAGACCGTTG CTGTCCTCGA GGAGCGTCTT ACCCTGACCG AAGACAAACT GAGGACCTGC 
CTGGACAATC AGGTCCTGCT TATGCAACAA AACCAACAAT TAGACCGATC TGACGAGGAA 
TCCGAGGGAC CCCCGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296167.1
CDS	86..1546
Translation	MEDPTLERHFKGHKDVISCADFNPNNKQLATGSCDKSLMIWNLAPKARAFRFVGHTDVITGVNFAPSGSLVASSSRDQTVRLWTPSIKGESTVFKAHTASVRSVHFSRDGQRLVTASDDKSVKVWGVERKKFLYSLNRHTNWVRCARFSPDGRLIASCGDDRTVRLWDTSSHQCINIFTDYGGSATFVDFNSSGTCIASSGADNTIKIWDIRTNKLIQHYKVHNAGVNCFSFHPSGNYLISGSSDSTIKILDLLEGRLIYTLHGHKGPVLTVTFSRDGDLFASGGADSQVLMWKTNFDSLNYRELLSKHSKRVTPDPPPHLMDIYPRSHHRHHPQNGTVEINPVADTQSTDPQVVEVGRVVFSTTDARNYDGASSSRAQFTSGMDSGPFRTHTQAREEEDENQEERFAGGMTASPAERSGIPSSLTSTLENIVQQLDILTQTVAVLEERLTLTEDKLRTCLDNQVLLMQQNQQLDRSDEESEGPPL

Target ORF information:

RefSeq Version	XM_009297892.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297892.2

ORF Insert Sequence:

ATGGAGGATC CCACCCTTGA GAGGCATTTT AAAGGTCATA AAGATGTAAT TTCCTGCGCT 
GACTTCAACC CCAATAATAA ACAGCTTGCT ACTGGATCCT GTGATAAAAG CCTTATGATT 
TGGAACCTCG CTCCTAAAGC CCGGGCTTTC AGGTTTGTGG GTCACACAGA TGTCATTACA 
GGCGTTAATT TTGCTCCAAG CGGTTCATTG GTGGCATCTT CATCCAGAGA TCAAACAGTT 
CGATTGTGGA CACCCAGTAT AAAGGGTGAA TCGACTGTGT TCAAAGCCCA CACAGCAAGC 
GTTCGAAGCG TTCATTTCTC CAGAGATGGC CAAAGGCTGG TCACGGCCTC AGATGACAAG 
TCTGTGAAAG TATGGGGTGT TGAACGGAAA AAATTCCTCT ACTCTCTCAA CCGGCACACC 
AACTGGGTGC GCTGTGCGAG GTTTTCACCA GATGGTCGTC TCATTGCTTC CTGTGGTGAC 
GACAGGACGG TTCGCCTCTG GGATACATCC TCACATCAGT GCATCAACAT TTTCACAGAC 
TATGGAGGGT CTGCCACATT TGTAGACTTC AACTCCAGTG GCACGTGCAT TGCATCATCA 
GGTGCAGACA ACACAATAAA AATCTGGGAT ATTCGTACAA ACAAGTTAAT ACAACATTAC 
AAAGTCCATA ATGCAGGAGT CAACTGCTTT TCCTTCCATC CCTCTGGAAA CTATCTGATC 
AGTGGTTCGA GTGACAGTAC AATTAAGATC TTGGACCTGC TGGAGGGGAG GCTCATCTAC 
ACTCTTCATG GCCATAAGGG TCCAGTTTTG ACCGTGACGT TTTCTCGAGA TGGAGATCTG 
TTTGCATCAG GAGGTGCAGA CTCTCAGGTT CTGATGTGGA AGACCAACTT TGATTCGCTG 
AACTACAGAG AACTGTTGAG CAAACACAGC AAACGCGTGA CTCCAGATCC TCCTCCACAC 
CTGATGGACA TTTACCCTCG ATCACATCAC CGACACCACC CTCAGAACGG CACAGTAGAG 
ATTAATCCTG TTGCTGATAC ACAGTCTACT GACCCTCAGG TGGTTGAAGT GGGACGTGTT 
GTTTTCTCCA CAACGGATGC AAGAAACTAT GATGGTGCAT CAAGCAGCAG AGCTCAGTTC 
ACCTCAGGAA TGGACTCAGG TCCATTTCGG ACTCATACAC AAGCCAGAGA AGAAGAGGAT 
GAAAACCAAG AGGAAAGATT TGCTGGCGGC ATGACAGCAT CTCCAGCAGA GCGCTCTGGT 
ATTCCATCAA GCCTTACTAG CACTCTGGAG AACATTGTAC AGCAGCTGGA CATCCTGACC 
CAGACCGTTG CTGTCCTCGA GGAGCGTCTT ACCCTGACCG AAGACAAACT GAGGACCTGC 
CTGGACAATC AGGTCCTGCT TATGCAACAA AACCAACAAT TAGACCGATC TGACGAGGAA 
TCCGAGGGAC CCCCGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poc1b ( XM_009297892.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296167.1 Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40355
Clone ID Related Accession (Same CDS sequence)	XM_009297892.2 , XM_009297892.3
Accession Version	XM_009297892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	POC1 centriolar protein homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC CCACCCTTGA GAGGCATTTT AAAGGTCATA AAGATGTAAT TTCCTGCGCT 
GACTTCAACC CCAATAATAA ACAGCTTGCT ACTGGATCCT GTGATAAAAG CCTTATGATT 
TGGAACCTCG CTCCTAAAGC CCGGGCTTTC AGGTTTGTGG GTCACACAGA TGTCATTACA 
GGCGTTAATT TTGCTCCAAG CGGTTCATTG GTGGCATCTT CATCCAGAGA TCAAACAGTT 
CGATTGTGGA CACCCAGTAT AAAGGGTGAA TCGACTGTGT TCAAAGCCCA CACAGCAAGC 
GTTCGAAGCG TTCATTTCTC CAGAGATGGC CAAAGGCTGG TCACGGCCTC AGATGACAAG 
TCTGTGAAAG TATGGGGTGT TGAACGGAAA AAATTCCTCT ACTCTCTCAA CCGGCACACC 
AACTGGGTGC GCTGTGCGAG GTTTTCACCA GATGGTCGTC TCATTGCTTC CTGTGGTGAC 
GACAGGACGG TTCGCCTCTG GGATACATCC TCACATCAGT GCATCAACAT TTTCACAGAC 
TATGGAGGGT CTGCCACATT TGTAGACTTC AACTCCAGTG GCACGTGCAT TGCATCATCA 
GGTGCAGACA ACACAATAAA AATCTGGGAT ATTCGTACAA ACAAGTTAAT ACAACATTAC 
AAAGTCCATA ATGCAGGAGT CAACTGCTTT TCCTTCCATC CCTCTGGAAA CTATCTGATC 
AGTGGTTCGA GTGACAGTAC AATTAAGATC TTGGACCTGC TGGAGGGGAG GCTCATCTAC 
ACTCTTCATG GCCATAAGGG TCCAGTTTTG ACCGTGACGT TTTCTCGAGA TGGAGATCTG 
TTTGCATCAG GAGGTGCAGA CTCTCAGGTT CTGATGTGGA AGACCAACTT TGATTCGCTG 
AACTACAGAG AACTGTTGAG CAAACACAGC AAACGCGTGA CTCCAGATCC TCCTCCACAC 
CTGATGGACA TTTACCCTCG ATCACATCAC CGACACCACC CTCAGAACGG CACAGTAGAG 
ATTAATCCTG TTGCTGATAC ACAGTCTACT GACCCTCAGG TGGTTGAAGT GGGACGTGTT 
GTTTTCTCCA CAACGGATGC AAGAAACTAT GATGGTGCAT CAAGCAGCAG AGCTCAGTTC 
ACCTCAGGAA TGGACTCAGG TCCATTTCGG ACTCATACAC AAGCCAGAGA AGAAGAGGAT 
GAAAACCAAG AGGAAAGATT TGCTGGCGGC ATGACAGCAT CTCCAGCAGA GCGCTCTGGT 
ATTCCATCAA GCCTTACTAG CACTCTGGAG AACATTGTAC AGCAGCTGGA CATCCTGACC 
CAGACCGTTG CTGTCCTCGA GGAGCGTCTT ACCCTGACCG AAGACAAACT GAGGACCTGC 
CTGGACAATC AGGTCCTGCT TATGCAACAA AACCAACAAT TAGACCGATC TGACGAGGAA 
TCCGAGGGAC CCCCGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296167.1
CDS	89..1549
Translation	MEDPTLERHFKGHKDVISCADFNPNNKQLATGSCDKSLMIWNLAPKARAFRFVGHTDVITGVNFAPSGSLVASSSRDQTVRLWTPSIKGESTVFKAHTASVRSVHFSRDGQRLVTASDDKSVKVWGVERKKFLYSLNRHTNWVRCARFSPDGRLIASCGDDRTVRLWDTSSHQCINIFTDYGGSATFVDFNSSGTCIASSGADNTIKIWDIRTNKLIQHYKVHNAGVNCFSFHPSGNYLISGSSDSTIKILDLLEGRLIYTLHGHKGPVLTVTFSRDGDLFASGGADSQVLMWKTNFDSLNYRELLSKHSKRVTPDPPPHLMDIYPRSHHRHHPQNGTVEINPVADTQSTDPQVVEVGRVVFSTTDARNYDGASSSRAQFTSGMDSGPFRTHTQAREEEDENQEERFAGGMTASPAERSGIPSSLTSTLENIVQQLDILTQTVAVLEERLTLTEDKLRTCLDNQVLLMQQNQQLDRSDEESEGPPL

Target ORF information:

RefSeq Version	XM_009297892.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297892.3

ORF Insert Sequence:

ATGGAGGATC CCACCCTTGA GAGGCATTTT AAAGGTCATA AAGATGTAAT TTCCTGCGCT 
GACTTCAACC CCAATAATAA ACAGCTTGCT ACTGGATCCT GTGATAAAAG CCTTATGATT 
TGGAACCTCG CTCCTAAAGC CCGGGCTTTC AGGTTTGTGG GTCACACAGA TGTCATTACA 
GGCGTTAATT TTGCTCCAAG CGGTTCATTG GTGGCATCTT CATCCAGAGA TCAAACAGTT 
CGATTGTGGA CACCCAGTAT AAAGGGTGAA TCGACTGTGT TCAAAGCCCA CACAGCAAGC 
GTTCGAAGCG TTCATTTCTC CAGAGATGGC CAAAGGCTGG TCACGGCCTC AGATGACAAG 
TCTGTGAAAG TATGGGGTGT TGAACGGAAA AAATTCCTCT ACTCTCTCAA CCGGCACACC 
AACTGGGTGC GCTGTGCGAG GTTTTCACCA GATGGTCGTC TCATTGCTTC CTGTGGTGAC 
GACAGGACGG TTCGCCTCTG GGATACATCC TCACATCAGT GCATCAACAT TTTCACAGAC 
TATGGAGGGT CTGCCACATT TGTAGACTTC AACTCCAGTG GCACGTGCAT TGCATCATCA 
GGTGCAGACA ACACAATAAA AATCTGGGAT ATTCGTACAA ACAAGTTAAT ACAACATTAC 
AAAGTCCATA ATGCAGGAGT CAACTGCTTT TCCTTCCATC CCTCTGGAAA CTATCTGATC 
AGTGGTTCGA GTGACAGTAC AATTAAGATC TTGGACCTGC TGGAGGGGAG GCTCATCTAC 
ACTCTTCATG GCCATAAGGG TCCAGTTTTG ACCGTGACGT TTTCTCGAGA TGGAGATCTG 
TTTGCATCAG GAGGTGCAGA CTCTCAGGTT CTGATGTGGA AGACCAACTT TGATTCGCTG 
AACTACAGAG AACTGTTGAG CAAACACAGC AAACGCGTGA CTCCAGATCC TCCTCCACAC 
CTGATGGACA TTTACCCTCG ATCACATCAC CGACACCACC CTCAGAACGG CACAGTAGAG 
ATTAATCCTG TTGCTGATAC ACAGTCTACT GACCCTCAGG TGGTTGAAGT GGGACGTGTT 
GTTTTCTCCA CAACGGATGC AAGAAACTAT GATGGTGCAT CAAGCAGCAG AGCTCAGTTC 
ACCTCAGGAA TGGACTCAGG TCCATTTCGG ACTCATACAC AAGCCAGAGA AGAAGAGGAT 
GAAAACCAAG AGGAAAGATT TGCTGGCGGC ATGACAGCAT CTCCAGCAGA GCGCTCTGGT 
ATTCCATCAA GCCTTACTAG CACTCTGGAG AACATTGTAC AGCAGCTGGA CATCCTGACC 
CAGACCGTTG CTGTCCTCGA GGAGCGTCTT ACCCTGACCG AAGACAAACT GAGGACCTGC 
CTGGACAATC AGGTCCTGCT TATGCAACAA AACCAACAAT TAGACCGATC TGACGAGGAA 
TCCGAGGGAC CCCCGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poc1b ( XM_017354060.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209549.1 Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa57787
Clone ID Related Accession (Same CDS sequence)	XM_017354060.2 , XM_017354060.1
Accession Version	XM_017354060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	POC1 centriolar protein homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTCAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTCATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGGCACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCATCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TGTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAAACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGTT GCGTGTGGGG TGACAGACAG GAAGCAGGCG 
AAGAGGCTCT TCCTGTGTTG TAAAGAGCAG GAGATTGGGT GGCCGCGAGG TGTGGATACC 
AGCTTGAAGG CCTGCCACCG GCTCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209549.1
CDS	175..1404
Translation	MASVMEDPTLERHFKGHKDVISCADFNPNNKQLATGSCDKSLMIWNLAPKARAFRFVGHTDVITGVNFAPSGSLVASSSRDQTVRLWTPSIKGESTVFKAHTASVRSVHFSRDGQRLVTASDDKSVKVWGVERKKFLYSLNRHTNWVRCARFSPDGRLIASCGDDRTVRLWDTSSHQCINIFTDYGGSATFVDFNSSGTCIASSGADNTIKIWDIRTNKLIQHYKVHNAGVNCFSFHPSGNYLISGSSDSTIKILDLLEGRLIYTLHGHKGPVLTVTFSRDGDLFASGGADSQVLMWKTNFDSLNYRELLSKHSKRVTPDPPPHLMDIYPRSHHRHHPQNGTVEINPVADTQSTDPQVVEVGRVVFSTTVACGVTDRKQAKRLFLCCKEQEIGWPRGVDTSLKACHRLR

Target ORF information:

RefSeq Version	XM_017354060.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354060.2

ORF Insert Sequence:

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTCAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTCATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGGCACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCATCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TGTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAAACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGTT GCGTGTGGGG TGACAGACAG GAAGCAGGCG 
AAGAGGCTCT TCCTGTGTTG TAAAGAGCAG GAGATTGGGT GGCCGCGAGG TGTGGATACC 
AGCTTGAAGG CCTGCCACCG GCTCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poc1b ( XM_017354060.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209549.1 Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa57787
Clone ID Related Accession (Same CDS sequence)	XM_017354060.2 , XM_017354060.1
Accession Version	XM_017354060.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	POC1 centriolar protein homolog B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTCAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTCATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGGCACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCATCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TGTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAAACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGTT GCGTGTGGGG TGACAGACAG GAAGCAGGCG 
AAGAGGCTCT TCCTGTGTTG TAAAGAGCAG GAGATTGGGT GGCCGCGAGG TGTGGATACC 
AGCTTGAAGG CCTGCCACCG GCTCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209549.1
CDS	145..1374
Translation	MASVMEDPTLERHFKGHKDVISCADFNPNNKQLATGSCDKSLMIWNLAPKARAFRFVGHTDVITGVNFAPSGSLVASSSRDQTVRLWTPSIKGESTVFKAHTASVRSVHFSRDGQRLVTASDDKSVKVWGVERKKFLYSLNRHTNWVRCARFSPDGRLIASCGDDRTVRLWDTSSHQCINIFTDYGGSATFVDFNSSGTCIASSGADNTIKIWDIRTNKLIQHYKVHNAGVNCFSFHPSGNYLISGSSDSTIKILDLLEGRLIYTLHGHKGPVLTVTFSRDGDLFASGGADSQVLMWKTNFDSLNYRELLSKHSKRVTPDPPPHLMDIYPRSHHRHHPQNGTVEINPVADTQSTDPQVVEVGRVVFSTTVACGVTDRKQAKRLFLCCKEQEIGWPRGVDTSLKACHRLR

Target ORF information:

RefSeq Version	XM_017354060.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio POC1 centriolar protein B (poc1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354060.1

ORF Insert Sequence:

ATGGCGTCTG TAATGGAGGA TCCCACCCTT GAGAGGCATT TTAAAGGTCA TAAAGATGTA 
ATTTCCTGCG CTGACTTCAA CCCCAATAAT AAACAGCTTG CTACTGGATC CTGTGATAAA 
AGCCTTATGA TTTGGAACCT CGCTCCTAAA GCCCGGGCTT TCAGGTTTGT GGGTCACACA 
GATGTCATTA CAGGCGTTAA TTTTGCTCCA AGCGGTTCAT TGGTGGCATC TTCATCCAGA 
GATCAAACAG TTCGATTGTG GACACCCAGT ATAAAGGGTG AATCGACTGT GTTCAAAGCC 
CACACAGCAA GCGTTCGAAG CGTTCATTTC TCCAGAGATG GCCAAAGGCT GGTCACGGCC 
TCAGATGACA AGTCTGTGAA AGTATGGGGT GTTGAACGGA AAAAATTCCT CTACTCTCTC 
AACCGGCACA CCAACTGGGT GCGCTGTGCG AGGTTTTCAC CAGATGGTCG TCTCATTGCT 
TCCTGTGGTG ACGACAGGAC GGTTCGCCTC TGGGATACAT CCTCACATCA GTGCATCAAC 
ATTTTCACAG ACTATGGAGG GTCTGCCACA TTTGTAGACT TCAACTCCAG TGGCACGTGC 
ATTGCATCAT CAGGTGCAGA CAACACAATA AAAATCTGGG ATATTCGTAC AAACAAGTTA 
ATACAACATT ACAAAGTCCA TAATGCAGGA GTCAACTGCT TTTCCTTCCA TCCCTCTGGA 
AACTATCTGA TCAGTGGTTC GAGTGACAGT ACAATTAAGA TCTTGGACCT GCTGGAGGGG 
AGGCTCATCT ACACTCTTCA TGGCCATAAG GGTCCAGTTT TGACCGTGAC GTTTTCTCGA 
GATGGAGATC TGTTTGCATC AGGAGGTGCA GACTCTCAGG TTCTGATGTG GAAGACCAAC 
TTTGATTCGC TGAACTACAG AGAACTGTTG AGCAAACACA GCAAACGCGT GACTCCAGAT 
CCTCCTCCAC ACCTGATGGA CATTTACCCT CGATCACATC ACCGACACCA CCCTCAGAAC 
GGCACAGTAG AGATTAATCC TGTTGCTGAT ACACAGTCTA CTGACCCTCA GGTGGTTGAA 
GTGGGACGTG TTGTTTTCTC CACAACGGTT GCGTGTGGGG TGACAGACAG GAAGCAGGCG 
AAGAGGCTCT TCCTGTGTTG TAAAGAGCAG GAGATTGGGT GGCCGCGAGG TGTGGATACC 
AGCTTGAAGG CCTGCCACCG GCTCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Knockdown of poc1b causes abnormal photoreceptor sensory cilium and vision impairment in zebrafish.
Biochemical and biophysical research communications465(4)651-7(2015 Oct)
Zhang C,Zhang Q,Wang F,Liu Q

Mutation of POC1B in a severe syndromic retinal ciliopathy.
Human mutation35(10)1153-62(2014 Oct)
Beck BB,Phillips JB,Bartram MP,Wegner J,Thoenes M,Pannes A,Sampson J,Heller R,G?bel H,Koerber F,Neugebauer A,Hedergott A,N?rnberg G,N?rnberg P,Thiele H,Altm?ller J,Toliat MR,Staubach S,Boycott KM,Valente EM,Janecke AR,Eisenberger T,Bergmann C,Tebbe L,Wang Y,Wu Y,Fry AM,Westerfield M,Wolfrum U,Bolz HJ

Disruption of the basal body protein POC1B results in autosomal-recessive cone-rod dystrophy.
American journal of human genetics95(2)131-42(2014 Aug)
Roosing S,Lamers IJ,de Vrieze E,van den Born LI,Lambertus S,Arts HH,,Peters TA,Hoyng CB,Kremer H,Hetterschijt L,Letteboer SJ,van Wijk E,Roepman R,den Hollander AI,Cremers FP

A highly conserved Poc1 protein characterized in embryos of the hydrozoan Clytia hemisphaerica: localization and functional studies.
PloS one5(11)e13994(2010 Nov)
Fourrage C,Chevalier S,Houliston E

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poc1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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