apc cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	apc
Entrez Gene ID	386762
Full Name	adenomatous polyposis coli
Synonyms	cb965,im:7150932,im:7152872
General protein information	Preferred Names adenomatous polyposis coli Names adenomatous polyposis coli protein adenomatosis polyposis coli
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357938.1	XP_017213427.1	adenomatous polyposis coli protein isoform X1
XM_005155523.3	XP_005155580.1	adenomatous polyposis coli protein isoform X1
XM_017357938.2	XP_017213427.1	adenomatous polyposis coli protein isoform X1
NM_001143840.1	NP_001137312.1	adenomatous polyposis coli protein
XM_005155523.4	XP_005155580.1	adenomatous polyposis coli protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APC	XP_005618091.1
Gallus gallus (chicken)	APC	XP_004949340.1
Danio rerio (zebrafish)	apc	NP_001137312.1
Homo sapiens (human)	APC	NP_001120982.1
Bos taurus (cattle)	APC	NP_001069454.2
Mus musculus (house mouse)	Apc	NP_031488.2
Rattus norvegicus (Norway rat)	Apc	NP_036631.1
Xenopus tropicalis (tropical clawed frog)	apc	XP_004910521.1
Pan troglodytes (chimpanzee)	APC	XP_001143965.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence conservation of protein binding segments in intrinsically disordered regions.
Ota H, Fukuchi S
Biochemical and biophysical research communications494(3-4)602-607(2017 12)

A metabolic switch controls intestinal differentiation downstream of
Sandoval IT, Delacruz RG, Miller BN, Hill S, Olson KA, Gabriel AE, Boyd K, Satterfield C, Remmen HV, Rutter J, Jones DA
eLife6(2017 04)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Apc mutations activate mechanistic target of rapamycin complex 1 in mice and zebrafish
Title: Oncogenic mutations in adenomatous polyposis coli (Apc) activate mechanistic target of rapamycin complex 1 (mTORC1) in mice and zebrafish.

Mutations in APC, a negative regulator of the Wnt/ss-catenin pathway, can cause cancer as well as profound developmental defects.
Title: Progenitor expansion in apc mutants is mediated by Jak/Stat signaling.

Study shows that homozygous loss of APC causes robust upregulation of the components of the demethylase and that this impairs the proper differentiation of intestinal cells.
Title: DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC.

Data show a loss of adenomatous polyposis coli (apc) results in an expanded ciliary marginal zone in the zebrafish eye.
Title: Loss of adenomatous polyposis coli (apc) results in an expanded ciliary marginal zone in the zebrafish eye.

Apc1-mediated restriction of Wnt/beta-catenin signalling is required for maintenance of local organizers and tectal integrity.
Title: Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival.

The following apc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the apc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02710	NM_001143840.1 Latest version!	Danio rerio APC regulator of WNT signaling pathway (apc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa15669	XM_005155523.4 Latest version!	Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa15669	XM_017357938.2 Latest version!	Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa15669	XM_017357938.1	Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa15669	XM_005155523.3	Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

apc ( NM_001143840.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001137312.1 Danio rerio APC regulator of WNT signaling pathway (apc), mRNA.

Price & Availability↑

CloneID	ODa02710
Clone ID Related Accession (Same CDS sequence)	NM_001143840.1
Accession Version	NM_001143840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	adenomatous polyposis coli protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU915637.3 and CU861476.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGAGTAGCGG TAAGCGATAT 
CGGGAGCGCG TCAAGCATGA GCCGCTGAGT CAGACTGAAG GATCTCATGC AGCAGGAGAC 
GCAGGCGCCG CCGCTGCTGC TTCTGTATGC TCGCAGGGCT CAGCTTCTCG TGTGGATCAT 
GACTCGGCCA GTGAGATGAG CAGTGCGGGC AGTTACTCTG TTCCACGCCG ACTGACCAGC 
CATCTGGGCA CGAAGGTGGA AATGGTGTAC TCTCTGCTGT CCATGCTGGG CACTCATGAT 
AAAGACGACA TGTCCCGAAC ACTGCTGGCC ATGTCCAGCT CTCAGGACAG CTGTATCGCC 
ATGCGTCAGT CGGGCTGCCT GCCCCTGCTC ATCCAGCTCC TGCACGGAAA CGACAAGGAC 
TCTGTGCTGC TGGGAAACTC ACGTGGCAGT AAAGAGGCTC GCGCCCGAGC CAGCGCCGCC 
CTGCACAACA TCATTCACTC ACAGCCGGAC GACAAGCGCG GACGCAGAGA GATCCGCGTA 
CTGCACCTGC TGGAGCAGAT CCGGGCCTAC TGCGAGACCT GCTGGGAGTG GCAGGAGAGC 
CACGAGCGAG GTGTGGACCA GGACAAAAAC CCCATGCCTT CGCCGGTGGA GCATCAGATT 
TGCCCGGCGG TTTGTGTCCT CATGAAGCTT TCCTTTGATG AAGAGCACAG ACACGCCATG 
AACGAGCTTG GCGGTCTTCA GGCGATCGGG GAGCTGCTGC AGGTGGACTG CGAGATTTAC 
GGTCTTACCA ATGATCACTA CAGTGTTACA CTAAGACGAT ATGCTGGGAT GGCTCTGACT 
AATCTGACCT TTGGAGATGT TGCTAACAAG GCAACACTTT GCTCAATGAA GGGTTGCATG 
AGGGCCATGG TGGCTCAACT GAAGTCAGAA AGCGAGGACC TACAGCAGGT AATTGCCAGT 
GTTTTGCGGA ACCTCTCATG GCGAGCTGAT GTGAACAGTA AGAAGATTTT GCGAGAAGTA 
GGCAGTGTGC GGGCTCTGAT GGAGTGTGCC TTAGAGGTGC AGAAGGAGTC CACATTAAAA 
AGTGTCCTGA GTGCCCTTTG GAACTTGTCG GCTCACTGCA CTGAAAACAA AGCTGATATC 
TGCACCGTTC CCGGAGCTTT GGCGTTTTTA GTCAGCACTT TAACCTACCG AAGCCAAACC 
AACACCCTTG CCATAATCGA AAGTGGAGGT GGCATTCTGA GAAACGTGTC AAGCCTCATC 
GCCACAAACG AAGAGCACAG GCAAATATTG AGGGAAAACA GCTGTCTTCA GACTCTTCTT 
CAGCACCTTA AATCACACAG TCTGACGATA GTGAGCAACG CCTGTGGGAC GCTGTGGAAC 
CTCTCCGCTC GCAACGCAAA GGATCAGGAG GCATTATGGG ACATGGGTGC AGTCAGCATG 
CTGAAAAACC TCATTCACTC CAAGCACAAG ATGATCGCTA TGGGAAGCGC TGCTGCTTTG 
AGAAACCTCA TGGCAAATCG GCCTGCCAAG TATAAGGATG CCAACATAAT GTCTCCTGGG 
TCCAGTCTGC CGTCCCTTCA TGTAAGAAAA CAGAAAGCAC TAATTGAAGA GCTAGATGCA 
CAGCACCTCT CTGAAACCTT CGATAACATC GATAACTTGA GTCCAAAAGC CTCTCATAGG 
GTCAAGCCAA GACATAAACA TAATGTCTAC GGTGATTATG ATGCCGTCTG TCGATCAGAT 
GGCTATAACC CCAATGGTGT TGGTGTTCGC TCGCCTTACA TGAACACTCC GGTGCTCTCA 
AGCCCGTCCT CTAGAGACAA CCGAGGAAAT GCAGAAAGTG TCCGAGCCGA GAGAGACCGA 
AGTCTCGATC GAGAGCGAAG GGGGTTCCTT CCAGACGGTG AGGCTGCCAA ACGAATGATG 
CAGATTCCCA CGAGCGCAGC TCAGATTGCA GTAGTTATGG AGGAAGTACA GAACATGCAC 
TTGGGTATGG ATGACAGGTC AGCAGGATCC ACACCAGACC CTCACTCAGT GCAAGATGAC 
ATGATTCGTC GTCAGACTGC TGTTCATGGT CACCAAAACA TATACAGCTA TAGCAAAACA 
GACCCATCGG GCAGACCCTG CCCAATGCCC AAACTGGAGT ACAGAGCTTC TAATGACAGT 
CTGAATAGTG TCAACAGCAC TGACGGCTAC GGGAAAAGAG GTCAAATGAA ACCCTCAGTA 
GATTCCTATT CGGAGGACGA TGAAGGCAAA TGTTGCGTCT ACAGAAAATA TCCTGCGGAC 
CTTGCCCATA AGATACACAA TGCAAATCAC ATGGAAGATG ATAATGGAGA TCTGGACACA 
CCTATTAACT ACAGTTTGAA GTATTCTGAT GAACAGCTCA ACTCTGGTCG ACAAAGCCCG 
AGCCAGAATG AAAGGTGGGC AAGGCCTAAG CTCCTCGATG ATGAAATGAA ACGGCCAGAT 
CAGAAACCAC CGCGATCACA AAGCCCAGGA TACCCTATGT ACACTGAAGG CAGCAGCGAG 
GGAGAGGATA AACCCAAAAA GTACCAACCC AGGTTTGTTC AACAGGACCT GCCTGCATTT 
CGGTCAAGGG GCTCCAATGA GCAAATTAGT AGCGGGTCAA GTCATGGACT TAACAAGAAG 
ATCTCTCAAA CCATCTGTAG TGTCGATGAC TATGCAGATG ACAAACCAAC AAACTACAGC 
GAGCGCTACT CAGAAGAAGA ACAGCTCGAG GAACAAACTC CCAGCTACAG CATGAAATAC 
ACTGAGGATC ATCATGTAGA GCAGCCTATT GATTACAGTT TGAAGTACTC GGAAGCTCCT 
TCCAAGAAGG GCATGTTTAG TCATTCAAAG ACGTCTTCTG CACAGAGTTC TGCCAAAGAA 
CATTTAAGCC AGGATAGTTC TTCTTCTGTG GCATCTTTAA AGAATCAGGG CAGGCAAAAG 
CAGCTCCACC CTTCATCTGC TCAAAGCAGA TCCGGACCAA CAAGAGCAGT TCAAAAGAAC 
CCAACATGCA AGGCTCCCAC AATAAATCAA GAAACTTTAC AGACCTATTG TGTGGAAGAT 
ACTCCCATTT GTTTTTCACG TGGTAGCTCT TTATCGTCGT TGTCTTCAGA GGAGGATGAA 
ATGGAGAGCT GTAAGCGTAA TGTCAACTCG GCCAGTAACT ACCCAACTTT ACCTATATCA 
GAAAAACAAT CCACTAATAA TGTTGCAGCT GACCAACGCA CATCTGAGAG CCAATCATCA 
GTCCATTATG TTCGCGCCAA GCCTCCACGA CATCATTTAG GACATGGTGA TGGTTCCAGG 
CATCATAAAA CGGTGGAGTT TTCATCAGGG GCTAAATCAC CTTCTAAAAG TGGTGCCCAA 
ACACCTAAAA GTCCCCCTGA GCATTATGTA CAGGAGACTC CTCTAATGTT CAGCAGATGC 
ACTTCAGTCA GCTCCCTTGA CAGTTTTGAG AGTCATTCCA TTGCCAGTTC TGTGCAGAGT 
GAGCCATGCA GTGGGATGGT CAGTGGCATC ATCAGTCCGA GTGACTTACC TGATAGTCCT 
GGACAAACAA TGCCTCCAAG TCGCAGCAAG ACACCACCAC CTCCACCTCC GCGGTCCACG 
TCAGTAAAAC AGAAAGTTAC AGTGCCACCC CACACTGAAA AACATGATTT GGCGCCAAGA 
CATGCAGTTG TAAGTGCTGC CGTGCAAAAA GTGCAAGTTT TGCCAGATAA TGATACTCTT 
CTACATTTTG CCACAGAAAG CACTCCAGAT GGTTTCTCCT GCGCCTCGAG CCTTAGTGCG 
CTAAGTTTAG ATGAACCCTT TATTCAAAAA GATGTTGAGC TGAAGATAAT GCCTCCTGTT 
CATGAGGACG ACCATAGTAT TGAGGCAGAG CCTGAGATGG AAGATATGCA TGAGCCCAAA 
GTTCAGGAGA AATCACCGGC AACATCTGAA GCTGCAAAAG ACATCTTGGA TGACTCGGAT 
GATGATGACG ACACAGAAAT TCTGAATGCT TGTATTAACT CAGCAATGCC AACAAAATCA 
TCAAGAAAAC CGAAAAAGCA GTCGACATCA AGGATACCAC CACCCGTGGC TTGCAAACCG 
AGTCAGCTCC CTGTTTATAA ATTATTGCCA CCACAAAACA GAGGGCAACC GCAGAAGCAT 
GTGGCCCTAG CACACGGTGA GGACATGCCC CGTGTGTACT GTGTTGAGGG AACACCCATT 
AACTTCTCCA CAGCAACATC TCTCAGTGAC CTCACAATTG ATTCTCCTCC GAACGAGTTG 
GCAGGTATGG AAAGTTCAGC TCCCCATGTA GAAGCATCTG GTCAAAGACG GGACACTCTG 
CCTGAAGGGA AAAGCGCAGA GGCTAAAGAG ACCGGTTTAT CACCTCCCAT GCAGTCTGCT 
CTTGCTGAGA ATGAAGGAGA TGATATTCTT GCTGAGTGTA TCAATTCAGC AATGCCAAAA 
AGCAAAATAC ACAAGCCGTT TAGAGTGCAG AAGATGCCTG ATCAGGCTCA GCATCCATCC 
ACTGCCACTG GCAGTCTAGT TCAGCAGGAC CTGGAAAAGA AAAAGCCAAC ATCTCCTGTA 
AAACCTATGC CACAAAGTAG TGAATATAGA GCAAGGATGC TCAAACGGCC AGAAGCTAAT 
AATAGCTTGG CAGATCCAGC AACATATCCA GATAAAAATA AAGAAACCAG AAAGCAAGAA 
CCCAAAGTAG TCATTAGAGA CTTTGCTGAT AAGCCATCTA ATGCTGAGGA GAGGACACGT 
CCCGGATTTG CCTTCGATTC TCCACATCAT TACACACCAA TTGAGGGGAC ACCTTATTGC 
TTCTCTCGCA ATGATTCACT GAGTTCACTT GACTTTGAGG ATGAGGATCT TGACTTTTCT 
AAGGAAAAAG CTGTGCTGCG AAAAGATAAG GAGCAGAGAA AAGTTCCCCT TCTGAAGTGC 
AGTGTGGAAC AGCCAGCAAA TACCAACATG GTATCGACAT TTCAGACAGC TCCAACAAAA 
CCTCTTCAAA AAACAGTTTT TCCACAAGCA CCCAAAGAAA ATACTGTTGT GGTGTGTGAT 
GAAAAGCAAA AGTTTTCCAT TGAAGACACT CCTGTATGTT TTTCAAGAAA CTCCTCACTC 
AGCTCACTAA GCGACATTGA CCAAGAAAAC AACAATAAAG ATTGCTCTCA CAAAGATGAT 
GTAACTCAAA TGGAGGCACC TAGGCCACAA GCGTCTGGTT ATGCACCAAA AGCCTTCCAC 
GTGGAGGACA CCCCTGTGTG CTTCTCTAGA AATAGTTCCC TGAGCTCTCT CAGTATTGAT 
TCTGAAGATG ACCTTCTCCA AGAATGCATC AGTTCTGCAA TGCCAAAGAA AAAGAAACAG 
ACACCAAGGA GTAAGACAGA GGAATCCGGG GTAAAGGAAG AAAAGAGCAT GATGGCTGAT 
GGTATTTTAT CAGAGGAACC AGATCTTATA TTAGATCTCA CAGACACACA TAGTCCTATT 
TCAGAGCAAG CCTTATCACC AGACTCTGAA TCATTTGATT GGAAAGCCAT TCAGGAAGGT 
GCTAACTCTA TAGTCAGCAG TCTACATCAG GCTGCAGCGA GTCTCTCGAG ACAAGGATCC 
TCTGATTCAG ACTCTATTCT TTCTCTCAAA TCTGGAATCT CCATTGGGTC GCCTTTCCAC 
CTGCCTTTAA ATCAAGATGA TAAGCCTGCC CCAAACAAAG GGCCAAGAAT ACTGAAACCT 
GGTGAGAAAA GCAGCATAGA AGCCAAAAAG AAGGAGGAAG AGACTGCAAA GAGTCTGAAA 
GGAGGAAAGA AAGTGTATAA AAGTCTAATA ACAGGAAAGC CCCGACCCAG TCTTGAAAGC 
ATGGCCTCTC AGCACAGACA AGCTCAAGCT CCTGTGATCT CTCGAGGGAG GACAATGGTT 
CACGTTCCTG GCGTCCGGAG CAGCTCCCCT AGTACCAGTC CGGTCCCCAA AAAGCCCCCT 
CCACGTGGCC AAATGTCAAA ACCCCCATCT CAAGCGCCAG GTGCTGGAAG CTCTCCAAGA 
ACCATGAAAG TCCCACCAAG TTCTGAGCCT AGTCCTGCAT CTGGTCCACC ATCTTCCCAA 
GGAGGGTCAA GTAAAGCTTC CTCTCGCTCT GGGTCTCGAG ACTCCACACC CTCTAGACCA 
GTTCAGCAAT CTCTGACAAG GCCCATGCAG TCTCCTGGTC GTGCCTCTGT TTCACCTGGT 
AGGAATGGCT TGAGTCCTTC GAACAAATTA TCTCAGTTGC CTCAGTTGCC GCGCACTGCC 
TCTCCTAGTG CATCTTCAAC TAAATCTTCA GGATCTGGTC GCATGGCTTA CACTTCTCCT 
GGAAGGCAGT TGGTACAACC AACGCCAACA AAGCAGAGTG GTTTGCCAAG AAGTACAAGT 
GGAATTCCTA GAAGTGAATC TGCCTCAAAA ATCCTAAACC AGTGTGGTCC TTCAAAGAAG 
GCAGAATTGT CTAGAATGTC TTCAACAAAG TCTAGTGGTA GCGAGTCCGA CCGCTCGGAG 
AAGCCTGGAC TTGTTCGTCA ATCAACATTT ATCAAAGAAG CACCTAGTCC AACGTTAAAA 
AGAAAATTAG AGGAATCTGC ATCATTCGAG TCCTTATCTC CTTCATCTAC AAGCCAGTCA 
CAGACTCCAG TATCTAGTCC TTCCTTGCCA GATATGTCAC TTAGTCTACC CTATCAGGGA 
AGTGGTTGGA CAAAGGCCCC TCAGAGTCAG AACTCTGCAG AGAATGGTGA CGGCAAGTCA 
CTCAAAAGGC ATGACATTTC CCGATCCCAT TCGGAAAGTC CTTCCAGGCT CCCAATCAAC 
AGGACAGGCA CATGGAAAAG GGAGCACAGC AAACATTCAT CTTCTCTCCC AAGAGTTGGC 
ACTTGGAAAA GAACCGGCAG CAGCTCTTCA ATTCTCTCCG CGTCTTCTGA ATCCAGTGAA 
AAAGGGAGAA GTGAGGATGA GCGACAACCA ACTAACCCAC CTCAAAAATC AGGTAAAGAG 
GGGGGCCTGG AGCGCAAAGG AACATGGAGA AAAGCGAAGG GAAGTGAAAC CTCCTATGCA 
CCCATGTCAT TAGACTTGCA AGATCAAACC GGCGATGCCA TGTCCAAATC AGAAGATGTC 
TGGGTGAGAA TTGAAGACTG TCCTATAAAC AATCCACGGT CAAGTAAATC GCCCACCGCC 
TCCACACCTC CTGTTATTGA CAGCCTCCCA ATCAAAGGGC TTGCCTGCGA CAGGGACTCA 
AGTGAATCAC ACTCGAAACT AATGAGTGAG AATGCTGCTA TGAGCCACTT GGGTTCAGAA 
ACAAACCTGA ATTTACTCAG GAGTAGCGAG TCACTTGACA AAAAAGTCAC TGACATCAAG 
CCTGCACCAA GTAATCCAAA CATCGGCCCA GAGCTCCATG AGTTCCCGGT TTCTGAACGC 
ACACCATTTA GCTCAACTAA CTCCAGCAAA CACAGCTCCC CCAGTGGTGC AGTAGCTGCC 
AGGGTCAGTC CTTTTAATTA CACACCAAGC CCTAGAAAGA GTAGCGCTGA CGGCTCGACT 
CCTAGACCAT CTCAGATCCC AACTCCGATT TCAAGCAATG CAAAAAAACG AGACACTAAA 
GGAGACACCA CTGAAAGTGG GTCCTACATC GTAACCTCTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137312.1
CDS	1..8265
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLNKLETEASNMKEVLKQLQGSIDEDSKDSQGQIEFLERIKEMSLDPSGFSGVKLRSKASLQGSADSSPSPSPVSSCPRRGASSGGRDSAGYLEELEKERSLLVAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEDQLGTCQDMEKRAQGRVARIQQIEKDMLRIRTRLQAQSAESESSGKRYRERVKHEPLSQTEGSHAAGDAGAAAAASVCSQGSASRVDHDSASEMSSAGSYSVPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCIAMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQESHERGVDQDKNPMPSPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIGELLQVDCEIYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRAMVAQLKSESEDLQQVIASVLRNLSWRADVNSKKILREVGSVRALMECALEVQKESTLKSVLSALWNLSAHCTENKADICTVPGALAFLVSTLTYRSQTNTLAIIESGGGILRNVSSLIATNEEHRQILRENSCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALIEELDAQHLSETFDNIDNLSPKASHRVKPRHKHNVYGDYDAVCRSDGYNPNGVGVRSPYMNTPVLSSPSSRDNRGNAESVRAERDRSLDRERRGFLPDGEAAKRMMQIPTSAAQIAVVMEEVQNMHLGMDDRSAGSTPDPHSVQDDMIRRQTAVHGHQNIYSYSKTDPSGRPCPMPKLEYRASNDSLNSVNSTDGYGKRGQMKPSVDSYSEDDEGKCCVYRKYPADLAHKIHNANHMEDDNGDLDTPINYSLKYSDEQLNSGRQSPSQNERWARPKLLDDEMKRPDQKPPRSQSPGYPMYTEGSSEGEDKPKKYQPRFVQQDLPAFRSRGSNEQISSGSSHGLNKKISQTICSVDDYADDKPTNYSERYSEEEQLEEQTPSYSMKYTEDHHVEQPIDYSLKYSEAPSKKGMFSHSKTSSAQSSAKEHLSQDSSSSVASLKNQGRQKQLHPSSAQSRSGPTRAVQKNPTCKAPTINQETLQTYCVEDTPICFSRGSSLSSLSSEEDEMESCKRNVNSASNYPTLPISEKQSTNNVAADQRTSESQSSVHYVRAKPPRHHLGHGDGSRHHKTVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESHSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPPRSTSVKQKVTVPPHTEKHDLAPRHAVVSAAVQKVQVLPDNDTLLHFATESTPDGFSCASSLSALSLDEPFIQKDVELKIMPPVHEDDHSIEAEPEMEDMHEPKVQEKSPATSEAAKDILDDSDDDDDTEILNACINSAMPTKSSRKPKKQSTSRIPPPVACKPSQLPVYKLLPPQNRGQPQKHVALAHGEDMPRVYCVEGTPINFSTATSLSDLTIDSPPNELAGMESSAPHVEASGQRRDTLPEGKSAEAKETGLSPPMQSALAENEGDDILAECINSAMPKSKIHKPFRVQKMPDQAQHPSTATGSLVQQDLEKKKPTSPVKPMPQSSEYRARMLKRPEANNSLADPATYPDKNKETRKQEPKVVIRDFADKPSNAEERTRPGFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFEDEDLDFSKEKAVLRKDKEQRKVPLLKCSVEQPANTNMVSTFQTAPTKPLQKTVFPQAPKENTVVVCDEKQKFSIEDTPVCFSRNSSLSSLSDIDQENNNKDCSHKDDVTQMEAPRPQASGYAPKAFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKQTPRSKTEESGVKEEKSMMADGILSEEPDLILDLTDTHSPISEQALSPDSESFDWKAIQEGANSIVSSLHQAAASLSRQGSSDSDSILSLKSGISIGSPFHLPLNQDDKPAPNKGPRILKPGEKSSIEAKKKEEETAKSLKGGKKVYKSLITGKPRPSLESMASQHRQAQAPVISRGRTMVHVPGVRSSSPSTSPVPKKPPPRGQMSKPPSQAPGAGSSPRTMKVPPSSEPSPASGPPSSQGGSSKASSRSGSRDSTPSRPVQQSLTRPMQSPGRASVSPGRNGLSPSNKLSQLPQLPRTASPSASSTKSSGSGRMAYTSPGRQLVQPTPTKQSGLPRSTSGIPRSESASKILNQCGPSKKAELSRMSSTKSSGSESDRSEKPGLVRQSTFIKEAPSPTLKRKLEESASFESLSPSSTSQSQTPVSSPSLPDMSLSLPYQGSGWTKAPQSQNSAENGDGKSLKRHDISRSHSESPSRLPINRTGTWKREHSKHSSSLPRVGTWKRTGSSSSILSASSESSEKGRSEDERQPTNPPQKSGKEGGLERKGTWRKAKGSETSYAPMSLDLQDQTGDAMSKSEDVWVRIEDCPINNPRSSKSPTASTPPVIDSLPIKGLACDRDSSESHSKLMSENAAMSHLGSETNLNLLRSSESLDKKVTDIKPAPSNPNIGPELHEFPVSERTPFSSTNSSKHSSPSGAVAARVSPFNYTPSPRKSSADGSTPRPSQIPTPISSNAKKRDTKGDTTESGSYIVTSV

Target ORF information:

RefSeq Version	NM_001143840.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio APC regulator of WNT signaling pathway (apc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143840.1

ORF Insert Sequence:

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGAGTAGCGG TAAGCGATAT 
CGGGAGCGCG TCAAGCATGA GCCGCTGAGT CAGACTGAAG GATCTCATGC AGCAGGAGAC 
GCAGGCGCCG CCGCTGCTGC TTCTGTATGC TCGCAGGGCT CAGCTTCTCG TGTGGATCAT 
GACTCGGCCA GTGAGATGAG CAGTGCGGGC AGTTACTCTG TTCCACGCCG ACTGACCAGC 
CATCTGGGCA CGAAGGTGGA AATGGTGTAC TCTCTGCTGT CCATGCTGGG CACTCATGAT 
AAAGACGACA TGTCCCGAAC ACTGCTGGCC ATGTCCAGCT CTCAGGACAG CTGTATCGCC 
ATGCGTCAGT CGGGCTGCCT GCCCCTGCTC ATCCAGCTCC TGCACGGAAA CGACAAGGAC 
TCTGTGCTGC TGGGAAACTC ACGTGGCAGT AAAGAGGCTC GCGCCCGAGC CAGCGCCGCC 
CTGCACAACA TCATTCACTC ACAGCCGGAC GACAAGCGCG GACGCAGAGA GATCCGCGTA 
CTGCACCTGC TGGAGCAGAT CCGGGCCTAC TGCGAGACCT GCTGGGAGTG GCAGGAGAGC 
CACGAGCGAG GTGTGGACCA GGACAAAAAC CCCATGCCTT CGCCGGTGGA GCATCAGATT 
TGCCCGGCGG TTTGTGTCCT CATGAAGCTT TCCTTTGATG AAGAGCACAG ACACGCCATG 
AACGAGCTTG GCGGTCTTCA GGCGATCGGG GAGCTGCTGC AGGTGGACTG CGAGATTTAC 
GGTCTTACCA ATGATCACTA CAGTGTTACA CTAAGACGAT ATGCTGGGAT GGCTCTGACT 
AATCTGACCT TTGGAGATGT TGCTAACAAG GCAACACTTT GCTCAATGAA GGGTTGCATG 
AGGGCCATGG TGGCTCAACT GAAGTCAGAA AGCGAGGACC TACAGCAGGT AATTGCCAGT 
GTTTTGCGGA ACCTCTCATG GCGAGCTGAT GTGAACAGTA AGAAGATTTT GCGAGAAGTA 
GGCAGTGTGC GGGCTCTGAT GGAGTGTGCC TTAGAGGTGC AGAAGGAGTC CACATTAAAA 
AGTGTCCTGA GTGCCCTTTG GAACTTGTCG GCTCACTGCA CTGAAAACAA AGCTGATATC 
TGCACCGTTC CCGGAGCTTT GGCGTTTTTA GTCAGCACTT TAACCTACCG AAGCCAAACC 
AACACCCTTG CCATAATCGA AAGTGGAGGT GGCATTCTGA GAAACGTGTC AAGCCTCATC 
GCCACAAACG AAGAGCACAG GCAAATATTG AGGGAAAACA GCTGTCTTCA GACTCTTCTT 
CAGCACCTTA AATCACACAG TCTGACGATA GTGAGCAACG CCTGTGGGAC GCTGTGGAAC 
CTCTCCGCTC GCAACGCAAA GGATCAGGAG GCATTATGGG ACATGGGTGC AGTCAGCATG 
CTGAAAAACC TCATTCACTC CAAGCACAAG ATGATCGCTA TGGGAAGCGC TGCTGCTTTG 
AGAAACCTCA TGGCAAATCG GCCTGCCAAG TATAAGGATG CCAACATAAT GTCTCCTGGG 
TCCAGTCTGC CGTCCCTTCA TGTAAGAAAA CAGAAAGCAC TAATTGAAGA GCTAGATGCA 
CAGCACCTCT CTGAAACCTT CGATAACATC GATAACTTGA GTCCAAAAGC CTCTCATAGG 
GTCAAGCCAA GACATAAACA TAATGTCTAC GGTGATTATG ATGCCGTCTG TCGATCAGAT 
GGCTATAACC CCAATGGTGT TGGTGTTCGC TCGCCTTACA TGAACACTCC GGTGCTCTCA 
AGCCCGTCCT CTAGAGACAA CCGAGGAAAT GCAGAAAGTG TCCGAGCCGA GAGAGACCGA 
AGTCTCGATC GAGAGCGAAG GGGGTTCCTT CCAGACGGTG AGGCTGCCAA ACGAATGATG 
CAGATTCCCA CGAGCGCAGC TCAGATTGCA GTAGTTATGG AGGAAGTACA GAACATGCAC 
TTGGGTATGG ATGACAGGTC AGCAGGATCC ACACCAGACC CTCACTCAGT GCAAGATGAC 
ATGATTCGTC GTCAGACTGC TGTTCATGGT CACCAAAACA TATACAGCTA TAGCAAAACA 
GACCCATCGG GCAGACCCTG CCCAATGCCC AAACTGGAGT ACAGAGCTTC TAATGACAGT 
CTGAATAGTG TCAACAGCAC TGACGGCTAC GGGAAAAGAG GTCAAATGAA ACCCTCAGTA 
GATTCCTATT CGGAGGACGA TGAAGGCAAA TGTTGCGTCT ACAGAAAATA TCCTGCGGAC 
CTTGCCCATA AGATACACAA TGCAAATCAC ATGGAAGATG ATAATGGAGA TCTGGACACA 
CCTATTAACT ACAGTTTGAA GTATTCTGAT GAACAGCTCA ACTCTGGTCG ACAAAGCCCG 
AGCCAGAATG AAAGGTGGGC AAGGCCTAAG CTCCTCGATG ATGAAATGAA ACGGCCAGAT 
CAGAAACCAC CGCGATCACA AAGCCCAGGA TACCCTATGT ACACTGAAGG CAGCAGCGAG 
GGAGAGGATA AACCCAAAAA GTACCAACCC AGGTTTGTTC AACAGGACCT GCCTGCATTT 
CGGTCAAGGG GCTCCAATGA GCAAATTAGT AGCGGGTCAA GTCATGGACT TAACAAGAAG 
ATCTCTCAAA CCATCTGTAG TGTCGATGAC TATGCAGATG ACAAACCAAC AAACTACAGC 
GAGCGCTACT CAGAAGAAGA ACAGCTCGAG GAACAAACTC CCAGCTACAG CATGAAATAC 
ACTGAGGATC ATCATGTAGA GCAGCCTATT GATTACAGTT TGAAGTACTC GGAAGCTCCT 
TCCAAGAAGG GCATGTTTAG TCATTCAAAG ACGTCTTCTG CACAGAGTTC TGCCAAAGAA 
CATTTAAGCC AGGATAGTTC TTCTTCTGTG GCATCTTTAA AGAATCAGGG CAGGCAAAAG 
CAGCTCCACC CTTCATCTGC TCAAAGCAGA TCCGGACCAA CAAGAGCAGT TCAAAAGAAC 
CCAACATGCA AGGCTCCCAC AATAAATCAA GAAACTTTAC AGACCTATTG TGTGGAAGAT 
ACTCCCATTT GTTTTTCACG TGGTAGCTCT TTATCGTCGT TGTCTTCAGA GGAGGATGAA 
ATGGAGAGCT GTAAGCGTAA TGTCAACTCG GCCAGTAACT ACCCAACTTT ACCTATATCA 
GAAAAACAAT CCACTAATAA TGTTGCAGCT GACCAACGCA CATCTGAGAG CCAATCATCA 
GTCCATTATG TTCGCGCCAA GCCTCCACGA CATCATTTAG GACATGGTGA TGGTTCCAGG 
CATCATAAAA CGGTGGAGTT TTCATCAGGG GCTAAATCAC CTTCTAAAAG TGGTGCCCAA 
ACACCTAAAA GTCCCCCTGA GCATTATGTA CAGGAGACTC CTCTAATGTT CAGCAGATGC 
ACTTCAGTCA GCTCCCTTGA CAGTTTTGAG AGTCATTCCA TTGCCAGTTC TGTGCAGAGT 
GAGCCATGCA GTGGGATGGT CAGTGGCATC ATCAGTCCGA GTGACTTACC TGATAGTCCT 
GGACAAACAA TGCCTCCAAG TCGCAGCAAG ACACCACCAC CTCCACCTCC GCGGTCCACG 
TCAGTAAAAC AGAAAGTTAC AGTGCCACCC CACACTGAAA AACATGATTT GGCGCCAAGA 
CATGCAGTTG TAAGTGCTGC CGTGCAAAAA GTGCAAGTTT TGCCAGATAA TGATACTCTT 
CTACATTTTG CCACAGAAAG CACTCCAGAT GGTTTCTCCT GCGCCTCGAG CCTTAGTGCG 
CTAAGTTTAG ATGAACCCTT TATTCAAAAA GATGTTGAGC TGAAGATAAT GCCTCCTGTT 
CATGAGGACG ACCATAGTAT TGAGGCAGAG CCTGAGATGG AAGATATGCA TGAGCCCAAA 
GTTCAGGAGA AATCACCGGC AACATCTGAA GCTGCAAAAG ACATCTTGGA TGACTCGGAT 
GATGATGACG ACACAGAAAT TCTGAATGCT TGTATTAACT CAGCAATGCC AACAAAATCA 
TCAAGAAAAC CGAAAAAGCA GTCGACATCA AGGATACCAC CACCCGTGGC TTGCAAACCG 
AGTCAGCTCC CTGTTTATAA ATTATTGCCA CCACAAAACA GAGGGCAACC GCAGAAGCAT 
GTGGCCCTAG CACACGGTGA GGACATGCCC CGTGTGTACT GTGTTGAGGG AACACCCATT 
AACTTCTCCA CAGCAACATC TCTCAGTGAC CTCACAATTG ATTCTCCTCC GAACGAGTTG 
GCAGGTATGG AAAGTTCAGC TCCCCATGTA GAAGCATCTG GTCAAAGACG GGACACTCTG 
CCTGAAGGGA AAAGCGCAGA GGCTAAAGAG ACCGGTTTAT CACCTCCCAT GCAGTCTGCT 
CTTGCTGAGA ATGAAGGAGA TGATATTCTT GCTGAGTGTA TCAATTCAGC AATGCCAAAA 
AGCAAAATAC ACAAGCCGTT TAGAGTGCAG AAGATGCCTG ATCAGGCTCA GCATCCATCC 
ACTGCCACTG GCAGTCTAGT TCAGCAGGAC CTGGAAAAGA AAAAGCCAAC ATCTCCTGTA 
AAACCTATGC CACAAAGTAG TGAATATAGA GCAAGGATGC TCAAACGGCC AGAAGCTAAT 
AATAGCTTGG CAGATCCAGC AACATATCCA GATAAAAATA AAGAAACCAG AAAGCAAGAA 
CCCAAAGTAG TCATTAGAGA CTTTGCTGAT AAGCCATCTA ATGCTGAGGA GAGGACACGT 
CCCGGATTTG CCTTCGATTC TCCACATCAT TACACACCAA TTGAGGGGAC ACCTTATTGC 
TTCTCTCGCA ATGATTCACT GAGTTCACTT GACTTTGAGG ATGAGGATCT TGACTTTTCT 
AAGGAAAAAG CTGTGCTGCG AAAAGATAAG GAGCAGAGAA AAGTTCCCCT TCTGAAGTGC 
AGTGTGGAAC AGCCAGCAAA TACCAACATG GTATCGACAT TTCAGACAGC TCCAACAAAA 
CCTCTTCAAA AAACAGTTTT TCCACAAGCA CCCAAAGAAA ATACTGTTGT GGTGTGTGAT 
GAAAAGCAAA AGTTTTCCAT TGAAGACACT CCTGTATGTT TTTCAAGAAA CTCCTCACTC 
AGCTCACTAA GCGACATTGA CCAAGAAAAC AACAATAAAG ATTGCTCTCA CAAAGATGAT 
GTAACTCAAA TGGAGGCACC TAGGCCACAA GCGTCTGGTT ATGCACCAAA AGCCTTCCAC 
GTGGAGGACA CCCCTGTGTG CTTCTCTAGA AATAGTTCCC TGAGCTCTCT CAGTATTGAT 
TCTGAAGATG ACCTTCTCCA AGAATGCATC AGTTCTGCAA TGCCAAAGAA AAAGAAACAG 
ACACCAAGGA GTAAGACAGA GGAATCCGGG GTAAAGGAAG AAAAGAGCAT GATGGCTGAT 
GGTATTTTAT CAGAGGAACC AGATCTTATA TTAGATCTCA CAGACACACA TAGTCCTATT 
TCAGAGCAAG CCTTATCACC AGACTCTGAA TCATTTGATT GGAAAGCCAT TCAGGAAGGT 
GCTAACTCTA TAGTCAGCAG TCTACATCAG GCTGCAGCGA GTCTCTCGAG ACAAGGATCC 
TCTGATTCAG ACTCTATTCT TTCTCTCAAA TCTGGAATCT CCATTGGGTC GCCTTTCCAC 
CTGCCTTTAA ATCAAGATGA TAAGCCTGCC CCAAACAAAG GGCCAAGAAT ACTGAAACCT 
GGTGAGAAAA GCAGCATAGA AGCCAAAAAG AAGGAGGAAG AGACTGCAAA GAGTCTGAAA 
GGAGGAAAGA AAGTGTATAA AAGTCTAATA ACAGGAAAGC CCCGACCCAG TCTTGAAAGC 
ATGGCCTCTC AGCACAGACA AGCTCAAGCT CCTGTGATCT CTCGAGGGAG GACAATGGTT 
CACGTTCCTG GCGTCCGGAG CAGCTCCCCT AGTACCAGTC CGGTCCCCAA AAAGCCCCCT 
CCACGTGGCC AAATGTCAAA ACCCCCATCT CAAGCGCCAG GTGCTGGAAG CTCTCCAAGA 
ACCATGAAAG TCCCACCAAG TTCTGAGCCT AGTCCTGCAT CTGGTCCACC ATCTTCCCAA 
GGAGGGTCAA GTAAAGCTTC CTCTCGCTCT GGGTCTCGAG ACTCCACACC CTCTAGACCA 
GTTCAGCAAT CTCTGACAAG GCCCATGCAG TCTCCTGGTC GTGCCTCTGT TTCACCTGGT 
AGGAATGGCT TGAGTCCTTC GAACAAATTA TCTCAGTTGC CTCAGTTGCC GCGCACTGCC 
TCTCCTAGTG CATCTTCAAC TAAATCTTCA GGATCTGGTC GCATGGCTTA CACTTCTCCT 
GGAAGGCAGT TGGTACAACC AACGCCAACA AAGCAGAGTG GTTTGCCAAG AAGTACAAGT 
GGAATTCCTA GAAGTGAATC TGCCTCAAAA ATCCTAAACC AGTGTGGTCC TTCAAAGAAG 
GCAGAATTGT CTAGAATGTC TTCAACAAAG TCTAGTGGTA GCGAGTCCGA CCGCTCGGAG 
AAGCCTGGAC TTGTTCGTCA ATCAACATTT ATCAAAGAAG CACCTAGTCC AACGTTAAAA 
AGAAAATTAG AGGAATCTGC ATCATTCGAG TCCTTATCTC CTTCATCTAC AAGCCAGTCA 
CAGACTCCAG TATCTAGTCC TTCCTTGCCA GATATGTCAC TTAGTCTACC CTATCAGGGA 
AGTGGTTGGA CAAAGGCCCC TCAGAGTCAG AACTCTGCAG AGAATGGTGA CGGCAAGTCA 
CTCAAAAGGC ATGACATTTC CCGATCCCAT TCGGAAAGTC CTTCCAGGCT CCCAATCAAC 
AGGACAGGCA CATGGAAAAG GGAGCACAGC AAACATTCAT CTTCTCTCCC AAGAGTTGGC 
ACTTGGAAAA GAACCGGCAG CAGCTCTTCA ATTCTCTCCG CGTCTTCTGA ATCCAGTGAA 
AAAGGGAGAA GTGAGGATGA GCGACAACCA ACTAACCCAC CTCAAAAATC AGGTAAAGAG 
GGGGGCCTGG AGCGCAAAGG AACATGGAGA AAAGCGAAGG GAAGTGAAAC CTCCTATGCA 
CCCATGTCAT TAGACTTGCA AGATCAAACC GGCGATGCCA TGTCCAAATC AGAAGATGTC 
TGGGTGAGAA TTGAAGACTG TCCTATAAAC AATCCACGGT CAAGTAAATC GCCCACCGCC 
TCCACACCTC CTGTTATTGA CAGCCTCCCA ATCAAAGGGC TTGCCTGCGA CAGGGACTCA 
AGTGAATCAC ACTCGAAACT AATGAGTGAG AATGCTGCTA TGAGCCACTT GGGTTCAGAA 
ACAAACCTGA ATTTACTCAG GAGTAGCGAG TCACTTGACA AAAAAGTCAC TGACATCAAG 
CCTGCACCAA GTAATCCAAA CATCGGCCCA GAGCTCCATG AGTTCCCGGT TTCTGAACGC 
ACACCATTTA GCTCAACTAA CTCCAGCAAA CACAGCTCCC CCAGTGGTGC AGTAGCTGCC 
AGGGTCAGTC CTTTTAATTA CACACCAAGC CCTAGAAAGA GTAGCGCTGA CGGCTCGACT 
CCTAGACCAT CTCAGATCCC AACTCCGATT TCAAGCAATG CAAAAAAACG AGACACTAAA 
GGAGACACCA CTGAAAGTGG GTCCTACATC GTAACCTCTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

apc ( XM_017357938.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213427.1 Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.

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CloneID	ODa15669
Clone ID Related Accession (Same CDS sequence)	XM_017357938.1 , XM_005155523.3 , XM_017357938.2 , XM_005155523.4
Accession Version	XM_017357938.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	adenomatous polyposis coli protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213427.1
CDS	346..8583
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLNKLETEASNMKEVLKQLQGSIDEDSKDSQGQIEFLERIKEMSLDPSGFSGVKLRSKASLQGSADSSPSPSPVSSCPRRGASSGGRDSAGYLEELEKERSLLVAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEDQLGTCQDMEKRAQGRVARIQQIEKDMLRIRTRLQAQSAESEVKHEPLSQTEGSHAAGDAGAAAAASVCSQGSASRVDHDSASEMSSAGSYSVPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCIAMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQESHERGVDQDKNPMPSPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIGELLQVDCEIYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRAMVAQLKSESEDLQQVIASVLRNLSWRADVNSKKILREVGSVRALMECALEVQKESTLKSVLSALWNLSAHCTENKADICTVPGALAFLVSTLTYRSQTNTLAIIESGGGILRNVSSLIATNEEHRQILRENSCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALIEELDAQHLSETFDNIDNLSPKASHRVKPRHKHNVYGDYDAVCRSDGYNPNGVGVRSPYMNTPVLSSPSSRDNRGNAESVRAERDRSLDRERRGFLPDGEAAKRMMQIPTSAAQIAVVMEEVQNMHLGMDDRSAGSTPDPHSVQDDMIRRQTAVHGHQNIYSYSKTDPSGRPCPMPKLEYRASNDSLNSVNSTDGYGKRGQMKPSVDSYSEDDEGKCCVYRKYPADLAHKIHNANHMEDDNGDLDTPINYSLKYSDEQLNSGRQSPSQNERWARPKLLDDEMKRPDQKPPRSQSPGYPMYTEGSSEGEDKPKKYQPRFVQQDLPAFRSRGSNEQISSGSSHGLNKKISQTICSVDDYADDKPTNYSERYSEEEQLEEQTPSYSMKYTEDHHVEQPIDYSLKYSEAPSKKGMFSHSKTSSAQSSAKEHLSQDSSSSVASLKNQGRQKQLHPSSAQSRSGPTRAVQKNPTCKAPTINQETLQTYCVEDTPICFSRGSSLSSLSSEEDEMESCKRNVNSASNYPTLPISEKQSTNNVAADQRTSESQSSVHYVRAKPPRHHLGHGDGSRHHKTVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESHSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPPRSTSVKQKVTVPPHTEKHDLAPRHAVVSAAVQKVQVLPDNDTLLHFATESTPDGFSCASSLSALSLDEPFIQKDVELKIMPPVHEDDHSIEAEPEMEDMHEPKVQEKSPATSEAAKDILDDSDDDDDTEILNACINSAMPTKSSRKPKKQSTSRIPPPVACKPSQLPVYKLLPPQNRGQPQKHVALAHGEDMPRVYCVEGTPINFSTATSLSDLTIDSPPNELAGMESSAPHVEASGQRRDTLPEGKSAEAKETGLSPPMQSALAENEGDDILAECINSAMPKSKIHKPFRVQKMPDQAQHPSTATGSLVQQDLEKKKPTSPVKPMPQSSEYRARMLKRPEANNSLADPATYPDKNKETRKQEPKVVIRDFADKPSNAEERTRPGFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFEDEDLDFSKEKAVLRKDKEQRKVPLLKCSVEQPANTNMVSTFQTAPTKPLQKTVFPQAPKENTVVVCDEKQKFSIEDTPVCFSRNSSLSSLSDIDQENNNKDCSHKDDVTQMEAPRPQASGYAPKAFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKQTPRSKTEESGVKEEKSMMADGILSEEPDLILDLTDTHSPISEQALSPDSESFDWKAIQEGANSIVSSLHQAAASLSRQGSSDSDSILSLKSGISIGSPFHLPLNQDDKPAPNKGPRILKPGEKSSIEAKKKEEETAKSLKGGKKVYKSLITGKPRPSLESMASQHRQAQAPVISRGRTMVHVPGVRSSSPSTSPVPKKPPPRGQMSKPPSQAPGAGSSPRTMKVPPSSEPSPASGPPSSQGGSSKASSRSGSRDSTPSRPVQQSLTRPMQSPGRASVSPGRNGLSPSNKLSQLPQLPRTASPSASSTKSSGSGRMAYTSPGRQLVQPTPTKQSGLPRSTSGIPRSESASKILNQCGPSKKAELSRMSSTKSSGSESDRSEKPGLVRQSTFIKEAPSPTLKRKLEESASFESLSPSSTSQSQTPVSSPSLPDMSLSLPYQGSGWTKAPQSQNSAENGDGKSLKRHDISRSHSESPSRLPINRTGTWKREHSKHSSSLPRVGTWKRTGSSSSILSASSESSEKGRSEDERQPTNPPQKSGKEGGLERKGTWRKAKGSETSYAPMSLDLQDQTGDAMSKSEDVWVRIEDCPINNPRSSKSPTASTPPVIDSLPIKGLACDRDSSESHSKLMSENAAMSHLGSETNLNLLRSSESLDKKVTDIKPAPSNPNIGPELHEFPVSERTPFSSTNSSKHSSPSGAVAARVSPFNYTPSPRKSSADGSTPRPSQIPTPISSNAKKRDTKGDTTESGSYIVTSV

Target ORF information:

RefSeq Version	XM_017357938.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357938.1

ORF Insert Sequence:

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

apc ( XM_005155523.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155580.1 Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.

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CloneID	ODa15669
Clone ID Related Accession (Same CDS sequence)	XM_017357938.1 , XM_005155523.3 , XM_017357938.2 , XM_005155523.4
Accession Version	XM_005155523.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	adenomatous polyposis coli protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155580.1
CDS	310..8547
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLNKLETEASNMKEVLKQLQGSIDEDSKDSQGQIEFLERIKEMSLDPSGFSGVKLRSKASLQGSADSSPSPSPVSSCPRRGASSGGRDSAGYLEELEKERSLLVAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEDQLGTCQDMEKRAQGRVARIQQIEKDMLRIRTRLQAQSAESEVKHEPLSQTEGSHAAGDAGAAAAASVCSQGSASRVDHDSASEMSSAGSYSVPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCIAMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQESHERGVDQDKNPMPSPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIGELLQVDCEIYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRAMVAQLKSESEDLQQVIASVLRNLSWRADVNSKKILREVGSVRALMECALEVQKESTLKSVLSALWNLSAHCTENKADICTVPGALAFLVSTLTYRSQTNTLAIIESGGGILRNVSSLIATNEEHRQILRENSCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALIEELDAQHLSETFDNIDNLSPKASHRVKPRHKHNVYGDYDAVCRSDGYNPNGVGVRSPYMNTPVLSSPSSRDNRGNAESVRAERDRSLDRERRGFLPDGEAAKRMMQIPTSAAQIAVVMEEVQNMHLGMDDRSAGSTPDPHSVQDDMIRRQTAVHGHQNIYSYSKTDPSGRPCPMPKLEYRASNDSLNSVNSTDGYGKRGQMKPSVDSYSEDDEGKCCVYRKYPADLAHKIHNANHMEDDNGDLDTPINYSLKYSDEQLNSGRQSPSQNERWARPKLLDDEMKRPDQKPPRSQSPGYPMYTEGSSEGEDKPKKYQPRFVQQDLPAFRSRGSNEQISSGSSHGLNKKISQTICSVDDYADDKPTNYSERYSEEEQLEEQTPSYSMKYTEDHHVEQPIDYSLKYSEAPSKKGMFSHSKTSSAQSSAKEHLSQDSSSSVASLKNQGRQKQLHPSSAQSRSGPTRAVQKNPTCKAPTINQETLQTYCVEDTPICFSRGSSLSSLSSEEDEMESCKRNVNSASNYPTLPISEKQSTNNVAADQRTSESQSSVHYVRAKPPRHHLGHGDGSRHHKTVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESHSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPPRSTSVKQKVTVPPHTEKHDLAPRHAVVSAAVQKVQVLPDNDTLLHFATESTPDGFSCASSLSALSLDEPFIQKDVELKIMPPVHEDDHSIEAEPEMEDMHEPKVQEKSPATSEAAKDILDDSDDDDDTEILNACINSAMPTKSSRKPKKQSTSRIPPPVACKPSQLPVYKLLPPQNRGQPQKHVALAHGEDMPRVYCVEGTPINFSTATSLSDLTIDSPPNELAGMESSAPHVEASGQRRDTLPEGKSAEAKETGLSPPMQSALAENEGDDILAECINSAMPKSKIHKPFRVQKMPDQAQHPSTATGSLVQQDLEKKKPTSPVKPMPQSSEYRARMLKRPEANNSLADPATYPDKNKETRKQEPKVVIRDFADKPSNAEERTRPGFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFEDEDLDFSKEKAVLRKDKEQRKVPLLKCSVEQPANTNMVSTFQTAPTKPLQKTVFPQAPKENTVVVCDEKQKFSIEDTPVCFSRNSSLSSLSDIDQENNNKDCSHKDDVTQMEAPRPQASGYAPKAFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKQTPRSKTEESGVKEEKSMMADGILSEEPDLILDLTDTHSPISEQALSPDSESFDWKAIQEGANSIVSSLHQAAASLSRQGSSDSDSILSLKSGISIGSPFHLPLNQDDKPAPNKGPRILKPGEKSSIEAKKKEEETAKSLKGGKKVYKSLITGKPRPSLESMASQHRQAQAPVISRGRTMVHVPGVRSSSPSTSPVPKKPPPRGQMSKPPSQAPGAGSSPRTMKVPPSSEPSPASGPPSSQGGSSKASSRSGSRDSTPSRPVQQSLTRPMQSPGRASVSPGRNGLSPSNKLSQLPQLPRTASPSASSTKSSGSGRMAYTSPGRQLVQPTPTKQSGLPRSTSGIPRSESASKILNQCGPSKKAELSRMSSTKSSGSESDRSEKPGLVRQSTFIKEAPSPTLKRKLEESASFESLSPSSTSQSQTPVSSPSLPDMSLSLPYQGSGWTKAPQSQNSAENGDGKSLKRHDISRSHSESPSRLPINRTGTWKREHSKHSSSLPRVGTWKRTGSSSSILSASSESSEKGRSEDERQPTNPPQKSGKEGGLERKGTWRKAKGSETSYAPMSLDLQDQTGDAMSKSEDVWVRIEDCPINNPRSSKSPTASTPPVIDSLPIKGLACDRDSSESHSKLMSENAAMSHLGSETNLNLLRSSESLDKKVTDIKPAPSNPNIGPELHEFPVSERTPFSSTNSSKHSSPSGAVAARVSPFNYTPSPRKSSADGSTPRPSQIPTPISSNAKKRDTKGDTTESGSYIVTSV

Target ORF information:

RefSeq Version	XM_005155523.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155523.3

ORF Insert Sequence:

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

apc ( XM_017357938.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213427.1 Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.

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CloneID	ODa15669
Clone ID Related Accession (Same CDS sequence)	XM_017357938.1 , XM_005155523.3 , XM_017357938.2 , XM_005155523.4
Accession Version	XM_017357938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenomatous polyposis coli protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213427.1
CDS	308..8545
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLNKLETEASNMKEVLKQLQGSIDEDSKDSQGQIEFLERIKEMSLDPSGFSGVKLRSKASLQGSADSSPSPSPVSSCPRRGASSGGRDSAGYLEELEKERSLLVAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEDQLGTCQDMEKRAQGRVARIQQIEKDMLRIRTRLQAQSAESEVKHEPLSQTEGSHAAGDAGAAAAASVCSQGSASRVDHDSASEMSSAGSYSVPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCIAMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQESHERGVDQDKNPMPSPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIGELLQVDCEIYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRAMVAQLKSESEDLQQVIASVLRNLSWRADVNSKKILREVGSVRALMECALEVQKESTLKSVLSALWNLSAHCTENKADICTVPGALAFLVSTLTYRSQTNTLAIIESGGGILRNVSSLIATNEEHRQILRENSCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALIEELDAQHLSETFDNIDNLSPKASHRVKPRHKHNVYGDYDAVCRSDGYNPNGVGVRSPYMNTPVLSSPSSRDNRGNAESVRAERDRSLDRERRGFLPDGEAAKRMMQIPTSAAQIAVVMEEVQNMHLGMDDRSAGSTPDPHSVQDDMIRRQTAVHGHQNIYSYSKTDPSGRPCPMPKLEYRASNDSLNSVNSTDGYGKRGQMKPSVDSYSEDDEGKCCVYRKYPADLAHKIHNANHMEDDNGDLDTPINYSLKYSDEQLNSGRQSPSQNERWARPKLLDDEMKRPDQKPPRSQSPGYPMYTEGSSEGEDKPKKYQPRFVQQDLPAFRSRGSNEQISSGSSHGLNKKISQTICSVDDYADDKPTNYSERYSEEEQLEEQTPSYSMKYTEDHHVEQPIDYSLKYSEAPSKKGMFSHSKTSSAQSSAKEHLSQDSSSSVASLKNQGRQKQLHPSSAQSRSGPTRAVQKNPTCKAPTINQETLQTYCVEDTPICFSRGSSLSSLSSEEDEMESCKRNVNSASNYPTLPISEKQSTNNVAADQRTSESQSSVHYVRAKPPRHHLGHGDGSRHHKTVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESHSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPPRSTSVKQKVTVPPHTEKHDLAPRHAVVSAAVQKVQVLPDNDTLLHFATESTPDGFSCASSLSALSLDEPFIQKDVELKIMPPVHEDDHSIEAEPEMEDMHEPKVQEKSPATSEAAKDILDDSDDDDDTEILNACINSAMPTKSSRKPKKQSTSRIPPPVACKPSQLPVYKLLPPQNRGQPQKHVALAHGEDMPRVYCVEGTPINFSTATSLSDLTIDSPPNELAGMESSAPHVEASGQRRDTLPEGKSAEAKETGLSPPMQSALAENEGDDILAECINSAMPKSKIHKPFRVQKMPDQAQHPSTATGSLVQQDLEKKKPTSPVKPMPQSSEYRARMLKRPEANNSLADPATYPDKNKETRKQEPKVVIRDFADKPSNAEERTRPGFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFEDEDLDFSKEKAVLRKDKEQRKVPLLKCSVEQPANTNMVSTFQTAPTKPLQKTVFPQAPKENTVVVCDEKQKFSIEDTPVCFSRNSSLSSLSDIDQENNNKDCSHKDDVTQMEAPRPQASGYAPKAFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKQTPRSKTEESGVKEEKSMMADGILSEEPDLILDLTDTHSPISEQALSPDSESFDWKAIQEGANSIVSSLHQAAASLSRQGSSDSDSILSLKSGISIGSPFHLPLNQDDKPAPNKGPRILKPGEKSSIEAKKKEEETAKSLKGGKKVYKSLITGKPRPSLESMASQHRQAQAPVISRGRTMVHVPGVRSSSPSTSPVPKKPPPRGQMSKPPSQAPGAGSSPRTMKVPPSSEPSPASGPPSSQGGSSKASSRSGSRDSTPSRPVQQSLTRPMQSPGRASVSPGRNGLSPSNKLSQLPQLPRTASPSASSTKSSGSGRMAYTSPGRQLVQPTPTKQSGLPRSTSGIPRSESASKILNQCGPSKKAELSRMSSTKSSGSESDRSEKPGLVRQSTFIKEAPSPTLKRKLEESASFESLSPSSTSQSQTPVSSPSLPDMSLSLPYQGSGWTKAPQSQNSAENGDGKSLKRHDISRSHSESPSRLPINRTGTWKREHSKHSSSLPRVGTWKRTGSSSSILSASSESSEKGRSEDERQPTNPPQKSGKEGGLERKGTWRKAKGSETSYAPMSLDLQDQTGDAMSKSEDVWVRIEDCPINNPRSSKSPTASTPPVIDSLPIKGLACDRDSSESHSKLMSENAAMSHLGSETNLNLLRSSESLDKKVTDIKPAPSNPNIGPELHEFPVSERTPFSSTNSSKHSSPSGAVAARVSPFNYTPSPRKSSADGSTPRPSQIPTPISSNAKKRDTKGDTTESGSYIVTSV

Target ORF information:

RefSeq Version	XM_017357938.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenomatous polyposis coli (apc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357938.2

ORF Insert Sequence:

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

apc ( XM_005155523.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155580.1 Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.

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CloneID	ODa15669
Clone ID Related Accession (Same CDS sequence)	XM_017357938.1 , XM_005155523.3 , XM_017357938.2 , XM_005155523.4
Accession Version	XM_005155523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenomatous polyposis coli protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155523.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155580.1
CDS	167..8404
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLNKLETEASNMKEVLKQLQGSIDEDSKDSQGQIEFLERIKEMSLDPSGFSGVKLRSKASLQGSADSSPSPSPVSSCPRRGASSGGRDSAGYLEELEKERSLLVAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEDQLGTCQDMEKRAQGRVARIQQIEKDMLRIRTRLQAQSAESEVKHEPLSQTEGSHAAGDAGAAAAASVCSQGSASRVDHDSASEMSSAGSYSVPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCIAMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQESHERGVDQDKNPMPSPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIGELLQVDCEIYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRAMVAQLKSESEDLQQVIASVLRNLSWRADVNSKKILREVGSVRALMECALEVQKESTLKSVLSALWNLSAHCTENKADICTVPGALAFLVSTLTYRSQTNTLAIIESGGGILRNVSSLIATNEEHRQILRENSCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALIEELDAQHLSETFDNIDNLSPKASHRVKPRHKHNVYGDYDAVCRSDGYNPNGVGVRSPYMNTPVLSSPSSRDNRGNAESVRAERDRSLDRERRGFLPDGEAAKRMMQIPTSAAQIAVVMEEVQNMHLGMDDRSAGSTPDPHSVQDDMIRRQTAVHGHQNIYSYSKTDPSGRPCPMPKLEYRASNDSLNSVNSTDGYGKRGQMKPSVDSYSEDDEGKCCVYRKYPADLAHKIHNANHMEDDNGDLDTPINYSLKYSDEQLNSGRQSPSQNERWARPKLLDDEMKRPDQKPPRSQSPGYPMYTEGSSEGEDKPKKYQPRFVQQDLPAFRSRGSNEQISSGSSHGLNKKISQTICSVDDYADDKPTNYSERYSEEEQLEEQTPSYSMKYTEDHHVEQPIDYSLKYSEAPSKKGMFSHSKTSSAQSSAKEHLSQDSSSSVASLKNQGRQKQLHPSSAQSRSGPTRAVQKNPTCKAPTINQETLQTYCVEDTPICFSRGSSLSSLSSEEDEMESCKRNVNSASNYPTLPISEKQSTNNVAADQRTSESQSSVHYVRAKPPRHHLGHGDGSRHHKTVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESHSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPPRSTSVKQKVTVPPHTEKHDLAPRHAVVSAAVQKVQVLPDNDTLLHFATESTPDGFSCASSLSALSLDEPFIQKDVELKIMPPVHEDDHSIEAEPEMEDMHEPKVQEKSPATSEAAKDILDDSDDDDDTEILNACINSAMPTKSSRKPKKQSTSRIPPPVACKPSQLPVYKLLPPQNRGQPQKHVALAHGEDMPRVYCVEGTPINFSTATSLSDLTIDSPPNELAGMESSAPHVEASGQRRDTLPEGKSAEAKETGLSPPMQSALAENEGDDILAECINSAMPKSKIHKPFRVQKMPDQAQHPSTATGSLVQQDLEKKKPTSPVKPMPQSSEYRARMLKRPEANNSLADPATYPDKNKETRKQEPKVVIRDFADKPSNAEERTRPGFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFEDEDLDFSKEKAVLRKDKEQRKVPLLKCSVEQPANTNMVSTFQTAPTKPLQKTVFPQAPKENTVVVCDEKQKFSIEDTPVCFSRNSSLSSLSDIDQENNNKDCSHKDDVTQMEAPRPQASGYAPKAFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKQTPRSKTEESGVKEEKSMMADGILSEEPDLILDLTDTHSPISEQALSPDSESFDWKAIQEGANSIVSSLHQAAASLSRQGSSDSDSILSLKSGISIGSPFHLPLNQDDKPAPNKGPRILKPGEKSSIEAKKKEEETAKSLKGGKKVYKSLITGKPRPSLESMASQHRQAQAPVISRGRTMVHVPGVRSSSPSTSPVPKKPPPRGQMSKPPSQAPGAGSSPRTMKVPPSSEPSPASGPPSSQGGSSKASSRSGSRDSTPSRPVQQSLTRPMQSPGRASVSPGRNGLSPSNKLSQLPQLPRTASPSASSTKSSGSGRMAYTSPGRQLVQPTPTKQSGLPRSTSGIPRSESASKILNQCGPSKKAELSRMSSTKSSGSESDRSEKPGLVRQSTFIKEAPSPTLKRKLEESASFESLSPSSTSQSQTPVSSPSLPDMSLSLPYQGSGWTKAPQSQNSAENGDGKSLKRHDISRSHSESPSRLPINRTGTWKREHSKHSSSLPRVGTWKRTGSSSSILSASSESSEKGRSEDERQPTNPPQKSGKEGGLERKGTWRKAKGSETSYAPMSLDLQDQTGDAMSKSEDVWVRIEDCPINNPRSSKSPTASTPPVIDSLPIKGLACDRDSSESHSKLMSENAAMSHLGSETNLNLLRSSESLDKKVTDIKPAPSNPNIGPELHEFPVSERTPFSSTNSSKHSSPSGAVAARVSPFNYTPSPRKSSADGSTPRPSQIPTPISSNAKKRDTKGDTTESGSYIVTSV

Target ORF information:

RefSeq Version	XM_005155523.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenomatous polyposis coli (apc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155523.4

ORF Insert Sequence:

ATGGCTGCAG CGTCTTATGA TCAGCTGCTG AAGCAAGTGG AGGCACTGAA GATGGAGAAC 
TCCAACCTGA GACAAGAGCT CGAGGACAAC TCCAACCACC TCAACAAACT GGAGACGGAG 
GCCTCTAATA TGAAGGAGGT TTTGAAGCAA CTGCAAGGAA GCATTGACGA GGACTCCAAA 
GATTCACAAG GACAGATCGA GTTTTTGGAG AGAATTAAAG AAATGAGTCT TGACCCGAGC 
GGTTTCTCTG GGGTGAAGCT GAGGTCTAAA GCGTCTCTGC AGGGCTCAGC AGACAGCAGC 
CCCAGCCCCA GCCCCGTCAG CTCTTGCCCT AGGAGAGGGG CGTCCAGCGG GGGCAGAGAC 
AGCGCTGGAT ATCTGGAGGA GCTGGAGAAA GAACGGTCTC TGTTGGTGGC AGAACTAGAG 
AAGGAAGAAA AGGAGAAGGA CTGGTATTAT GCCCAGCTGC AAAACCTCAC GAAAAGGATC 
GACAGTCTGC CACTGACGGA GAATTTTTCT CTCCAGACTG ATATGACTCG CAGGCAGCTG 
GAATACGAGG CCCGGCAGAT CAGAGCCGCG ATGGAGGATC AGCTGGGCAC GTGTCAGGAC 
ATGGAGAAGC GCGCACAGGG TCGTGTCGCT CGGATTCAAC AGATAGAAAA AGACATGCTG 
AGGATTCGGA CACGTTTACA GGCCCAGTCC GCTGAGTCAG AGGTCAAGCA TGAGCCGCTG 
AGTCAGACTG AAGGATCTCA TGCAGCAGGA GACGCAGGCG CCGCCGCTGC TGCTTCTGTA 
TGCTCGCAGG GCTCAGCTTC TCGTGTGGAT CATGACTCGG CCAGTGAGAT GAGCAGTGCG 
GGCAGTTACT CTGTTCCACG CCGACTGACC AGCCATCTGG GCACGAAGGT GGAAATGGTG 
TACTCTCTGC TGTCCATGCT GGGCACTCAT GATAAAGACG ACATGTCCCG AACACTGCTG 
GCCATGTCCA GCTCTCAGGA CAGCTGTATC GCCATGCGTC AGTCGGGCTG CCTGCCCCTG 
CTCATCCAGC TCCTGCACGG AAACGACAAG GACTCTGTGC TGCTGGGAAA CTCACGTGGC 
AGTAAAGAGG CTCGCGCCCG AGCCAGCGCC GCCCTGCACA ACATCATTCA CTCACAGCCG 
GACGACAAGC GCGGACGCAG AGAGATCCGC GTACTGCACC TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA GTGGCAGGAG AGCCACGAGC GAGGTGTGGA CCAGGACAAA 
AACCCCATGC CTTCGCCGGT GGAGCATCAG ATTTGCCCGG CGGTTTGTGT CCTCATGAAG 
CTTTCCTTTG ATGAAGAGCA CAGACACGCC ATGAACGAGC TTGGCGGTCT TCAGGCGATC 
GGGGAGCTGC TGCAGGTGGA CTGCGAGATT TACGGTCTTA CCAATGATCA CTACAGTGTT 
ACACTAAGAC GATATGCTGG GATGGCTCTG ACTAATCTGA CCTTTGGAGA TGTTGCTAAC 
AAGGCAACAC TTTGCTCAAT GAAGGGTTGC ATGAGGGCCA TGGTGGCTCA ACTGAAGTCA 
GAAAGCGAGG ACCTACAGCA GGTAATTGCC AGTGTTTTGC GGAACCTCTC ATGGCGAGCT 
GATGTGAACA GTAAGAAGAT TTTGCGAGAA GTAGGCAGTG TGCGGGCTCT GATGGAGTGT 
GCCTTAGAGG TGCAGAAGGA GTCCACATTA AAAAGTGTCC TGAGTGCCCT TTGGAACTTG 
TCGGCTCACT GCACTGAAAA CAAAGCTGAT ATCTGCACCG TTCCCGGAGC TTTGGCGTTT 
TTAGTCAGCA CTTTAACCTA CCGAAGCCAA ACCAACACCC TTGCCATAAT CGAAAGTGGA 
GGTGGCATTC TGAGAAACGT GTCAAGCCTC ATCGCCACAA ACGAAGAGCA CAGGCAAATA 
TTGAGGGAAA ACAGCTGTCT TCAGACTCTT CTTCAGCACC TTAAATCACA CAGTCTGACG 
ATAGTGAGCA ACGCCTGTGG GACGCTGTGG AACCTCTCCG CTCGCAACGC AAAGGATCAG 
GAGGCATTAT GGGACATGGG TGCAGTCAGC ATGCTGAAAA ACCTCATTCA CTCCAAGCAC 
AAGATGATCG CTATGGGAAG CGCTGCTGCT TTGAGAAACC TCATGGCAAA TCGGCCTGCC 
AAGTATAAGG ATGCCAACAT AATGTCTCCT GGGTCCAGTC TGCCGTCCCT TCATGTAAGA 
AAACAGAAAG CACTAATTGA AGAGCTAGAT GCACAGCACC TCTCTGAAAC CTTCGATAAC 
ATCGATAACT TGAGTCCAAA AGCCTCTCAT AGGGTCAAGC CAAGACATAA ACATAATGTC 
TACGGTGATT ATGATGCCGT CTGTCGATCA GATGGCTATA ACCCCAATGG TGTTGGTGTT 
CGCTCGCCTT ACATGAACAC TCCGGTGCTC TCAAGCCCGT CCTCTAGAGA CAACCGAGGA 
AATGCAGAAA GTGTCCGAGC CGAGAGAGAC CGAAGTCTCG ATCGAGAGCG AAGGGGGTTC 
CTTCCAGACG GTGAGGCTGC CAAACGAATG ATGCAGATTC CCACGAGCGC AGCTCAGATT 
GCAGTAGTTA TGGAGGAAGT ACAGAACATG CACTTGGGTA TGGATGACAG GTCAGCAGGA 
TCCACACCAG ACCCTCACTC AGTGCAAGAT GACATGATTC GTCGTCAGAC TGCTGTTCAT 
GGTCACCAAA ACATATACAG CTATAGCAAA ACAGACCCAT CGGGCAGACC CTGCCCAATG 
CCCAAACTGG AGTACAGAGC TTCTAATGAC AGTCTGAATA GTGTCAACAG CACTGACGGC 
TACGGGAAAA GAGGTCAAAT GAAACCCTCA GTAGATTCCT ATTCGGAGGA CGATGAAGGC 
AAATGTTGCG TCTACAGAAA ATATCCTGCG GACCTTGCCC ATAAGATACA CAATGCAAAT 
CACATGGAAG ATGATAATGG AGATCTGGAC ACACCTATTA ACTACAGTTT GAAGTATTCT 
GATGAACAGC TCAACTCTGG TCGACAAAGC CCGAGCCAGA ATGAAAGGTG GGCAAGGCCT 
AAGCTCCTCG ATGATGAAAT GAAACGGCCA GATCAGAAAC CACCGCGATC ACAAAGCCCA 
GGATACCCTA TGTACACTGA AGGCAGCAGC GAGGGAGAGG ATAAACCCAA AAAGTACCAA 
CCCAGGTTTG TTCAACAGGA CCTGCCTGCA TTTCGGTCAA GGGGCTCCAA TGAGCAAATT 
AGTAGCGGGT CAAGTCATGG ACTTAACAAG AAGATCTCTC AAACCATCTG TAGTGTCGAT 
GACTATGCAG ATGACAAACC AACAAACTAC AGCGAGCGCT ACTCAGAAGA AGAACAGCTC 
GAGGAACAAA CTCCCAGCTA CAGCATGAAA TACACTGAGG ATCATCATGT AGAGCAGCCT 
ATTGATTACA GTTTGAAGTA CTCGGAAGCT CCTTCCAAGA AGGGCATGTT TAGTCATTCA 
AAGACGTCTT CTGCACAGAG TTCTGCCAAA GAACATTTAA GCCAGGATAG TTCTTCTTCT 
GTGGCATCTT TAAAGAATCA GGGCAGGCAA AAGCAGCTCC ACCCTTCATC TGCTCAAAGC 
AGATCCGGAC CAACAAGAGC AGTTCAAAAG AACCCAACAT GCAAGGCTCC CACAATAAAT 
CAAGAAACTT TACAGACCTA TTGTGTGGAA GATACTCCCA TTTGTTTTTC ACGTGGTAGC 
TCTTTATCGT CGTTGTCTTC AGAGGAGGAT GAAATGGAGA GCTGTAAGCG TAATGTCAAC 
TCGGCCAGTA ACTACCCAAC TTTACCTATA TCAGAAAAAC AATCCACTAA TAATGTTGCA 
GCTGACCAAC GCACATCTGA GAGCCAATCA TCAGTCCATT ATGTTCGCGC CAAGCCTCCA 
CGACATCATT TAGGACATGG TGATGGTTCC AGGCATCATA AAACGGTGGA GTTTTCATCA 
GGGGCTAAAT CACCTTCTAA AAGTGGTGCC CAAACACCTA AAAGTCCCCC TGAGCATTAT 
GTACAGGAGA CTCCTCTAAT GTTCAGCAGA TGCACTTCAG TCAGCTCCCT TGACAGTTTT 
GAGAGTCATT CCATTGCCAG TTCTGTGCAG AGTGAGCCAT GCAGTGGGAT GGTCAGTGGC 
ATCATCAGTC CGAGTGACTT ACCTGATAGT CCTGGACAAA CAATGCCTCC AAGTCGCAGC 
AAGACACCAC CACCTCCACC TCCGCGGTCC ACGTCAGTAA AACAGAAAGT TACAGTGCCA 
CCCCACACTG AAAAACATGA TTTGGCGCCA AGACATGCAG TTGTAAGTGC TGCCGTGCAA 
AAAGTGCAAG TTTTGCCAGA TAATGATACT CTTCTACATT TTGCCACAGA AAGCACTCCA 
GATGGTTTCT CCTGCGCCTC GAGCCTTAGT GCGCTAAGTT TAGATGAACC CTTTATTCAA 
AAAGATGTTG AGCTGAAGAT AATGCCTCCT GTTCATGAGG ACGACCATAG TATTGAGGCA 
GAGCCTGAGA TGGAAGATAT GCATGAGCCC AAAGTTCAGG AGAAATCACC GGCAACATCT 
GAAGCTGCAA AAGACATCTT GGATGACTCG GATGATGATG ACGACACAGA AATTCTGAAT 
GCTTGTATTA ACTCAGCAAT GCCAACAAAA TCATCAAGAA AACCGAAAAA GCAGTCGACA 
TCAAGGATAC CACCACCCGT GGCTTGCAAA CCGAGTCAGC TCCCTGTTTA TAAATTATTG 
CCACCACAAA ACAGAGGGCA ACCGCAGAAG CATGTGGCCC TAGCACACGG TGAGGACATG 
CCCCGTGTGT ACTGTGTTGA GGGAACACCC ATTAACTTCT CCACAGCAAC ATCTCTCAGT 
GACCTCACAA TTGATTCTCC TCCGAACGAG TTGGCAGGTA TGGAAAGTTC AGCTCCCCAT 
GTAGAAGCAT CTGGTCAAAG ACGGGACACT CTGCCTGAAG GGAAAAGCGC AGAGGCTAAA 
GAGACCGGTT TATCACCTCC CATGCAGTCT GCTCTTGCTG AGAATGAAGG AGATGATATT 
CTTGCTGAGT GTATCAATTC AGCAATGCCA AAAAGCAAAA TACACAAGCC GTTTAGAGTG 
CAGAAGATGC CTGATCAGGC TCAGCATCCA TCCACTGCCA CTGGCAGTCT AGTTCAGCAG 
GACCTGGAAA AGAAAAAGCC AACATCTCCT GTAAAACCTA TGCCACAAAG TAGTGAATAT 
AGAGCAAGGA TGCTCAAACG GCCAGAAGCT AATAATAGCT TGGCAGATCC AGCAACATAT 
CCAGATAAAA ATAAAGAAAC CAGAAAGCAA GAACCCAAAG TAGTCATTAG AGACTTTGCT 
GATAAGCCAT CTAATGCTGA GGAGAGGACA CGTCCCGGAT TTGCCTTCGA TTCTCCACAT 
CATTACACAC CAATTGAGGG GACACCTTAT TGCTTCTCTC GCAATGATTC ACTGAGTTCA 
CTTGACTTTG AGGATGAGGA TCTTGACTTT TCTAAGGAAA AAGCTGTGCT GCGAAAAGAT 
AAGGAGCAGA GAAAAGTTCC CCTTCTGAAG TGCAGTGTGG AACAGCCAGC AAATACCAAC 
ATGGTATCGA CATTTCAGAC AGCTCCAACA AAACCTCTTC AAAAAACAGT TTTTCCACAA 
GCACCCAAAG AAAATACTGT TGTGGTGTGT GATGAAAAGC AAAAGTTTTC CATTGAAGAC 
ACTCCTGTAT GTTTTTCAAG AAACTCCTCA CTCAGCTCAC TAAGCGACAT TGACCAAGAA 
AACAACAATA AAGATTGCTC TCACAAAGAT GATGTAACTC AAATGGAGGC ACCTAGGCCA 
CAAGCGTCTG GTTATGCACC AAAAGCCTTC CACGTGGAGG ACACCCCTGT GTGCTTCTCT 
AGAAATAGTT CCCTGAGCTC TCTCAGTATT GATTCTGAAG ATGACCTTCT CCAAGAATGC 
ATCAGTTCTG CAATGCCAAA GAAAAAGAAA CAGACACCAA GGAGTAAGAC AGAGGAATCC 
GGGGTAAAGG AAGAAAAGAG CATGATGGCT GATGGTATTT TATCAGAGGA ACCAGATCTT 
ATATTAGATC TCACAGACAC ACATAGTCCT ATTTCAGAGC AAGCCTTATC ACCAGACTCT 
GAATCATTTG ATTGGAAAGC CATTCAGGAA GGTGCTAACT CTATAGTCAG CAGTCTACAT 
CAGGCTGCAG CGAGTCTCTC GAGACAAGGA TCCTCTGATT CAGACTCTAT TCTTTCTCTC 
AAATCTGGAA TCTCCATTGG GTCGCCTTTC CACCTGCCTT TAAATCAAGA TGATAAGCCT 
GCCCCAAACA AAGGGCCAAG AATACTGAAA CCTGGTGAGA AAAGCAGCAT AGAAGCCAAA 
AAGAAGGAGG AAGAGACTGC AAAGAGTCTG AAAGGAGGAA AGAAAGTGTA TAAAAGTCTA 
ATAACAGGAA AGCCCCGACC CAGTCTTGAA AGCATGGCCT CTCAGCACAG ACAAGCTCAA 
GCTCCTGTGA TCTCTCGAGG GAGGACAATG GTTCACGTTC CTGGCGTCCG GAGCAGCTCC 
CCTAGTACCA GTCCGGTCCC CAAAAAGCCC CCTCCACGTG GCCAAATGTC AAAACCCCCA 
TCTCAAGCGC CAGGTGCTGG AAGCTCTCCA AGAACCATGA AAGTCCCACC AAGTTCTGAG 
CCTAGTCCTG CATCTGGTCC ACCATCTTCC CAAGGAGGGT CAAGTAAAGC TTCCTCTCGC 
TCTGGGTCTC GAGACTCCAC ACCCTCTAGA CCAGTTCAGC AATCTCTGAC AAGGCCCATG 
CAGTCTCCTG GTCGTGCCTC TGTTTCACCT GGTAGGAATG GCTTGAGTCC TTCGAACAAA 
TTATCTCAGT TGCCTCAGTT GCCGCGCACT GCCTCTCCTA GTGCATCTTC AACTAAATCT 
TCAGGATCTG GTCGCATGGC TTACACTTCT CCTGGAAGGC AGTTGGTACA ACCAACGCCA 
ACAAAGCAGA GTGGTTTGCC AAGAAGTACA AGTGGAATTC CTAGAAGTGA ATCTGCCTCA 
AAAATCCTAA ACCAGTGTGG TCCTTCAAAG AAGGCAGAAT TGTCTAGAAT GTCTTCAACA 
AAGTCTAGTG GTAGCGAGTC CGACCGCTCG GAGAAGCCTG GACTTGTTCG TCAATCAACA 
TTTATCAAAG AAGCACCTAG TCCAACGTTA AAAAGAAAAT TAGAGGAATC TGCATCATTC 
GAGTCCTTAT CTCCTTCATC TACAAGCCAG TCACAGACTC CAGTATCTAG TCCTTCCTTG 
CCAGATATGT CACTTAGTCT ACCCTATCAG GGAAGTGGTT GGACAAAGGC CCCTCAGAGT 
CAGAACTCTG CAGAGAATGG TGACGGCAAG TCACTCAAAA GGCATGACAT TTCCCGATCC 
CATTCGGAAA GTCCTTCCAG GCTCCCAATC AACAGGACAG GCACATGGAA AAGGGAGCAC 
AGCAAACATT CATCTTCTCT CCCAAGAGTT GGCACTTGGA AAAGAACCGG CAGCAGCTCT 
TCAATTCTCT CCGCGTCTTC TGAATCCAGT GAAAAAGGGA GAAGTGAGGA TGAGCGACAA 
CCAACTAACC CACCTCAAAA ATCAGGTAAA GAGGGGGGCC TGGAGCGCAA AGGAACATGG 
AGAAAAGCGA AGGGAAGTGA AACCTCCTAT GCACCCATGT CATTAGACTT GCAAGATCAA 
ACCGGCGATG CCATGTCCAA ATCAGAAGAT GTCTGGGTGA GAATTGAAGA CTGTCCTATA 
AACAATCCAC GGTCAAGTAA ATCGCCCACC GCCTCCACAC CTCCTGTTAT TGACAGCCTC 
CCAATCAAAG GGCTTGCCTG CGACAGGGAC TCAAGTGAAT CACACTCGAA ACTAATGAGT 
GAGAATGCTG CTATGAGCCA CTTGGGTTCA GAAACAAACC TGAATTTACT CAGGAGTAGC 
GAGTCACTTG ACAAAAAAGT CACTGACATC AAGCCTGCAC CAAGTAATCC AAACATCGGC 
CCAGAGCTCC ATGAGTTCCC GGTTTCTGAA CGCACACCAT TTAGCTCAAC TAACTCCAGC 
AAACACAGCT CCCCCAGTGG TGCAGTAGCT GCCAGGGTCA GTCCTTTTAA TTACACACCA 
AGCCCTAGAA AGAGTAGCGC TGACGGCTCG ACTCCTAGAC CATCTCAGAT CCCAACTCCG 
ATTTCAAGCA ATGCAAAAAA ACGAGACACT AAAGGAGACA CCACTGAAAG TGGGTCCTAC 
ATCGTAACCT CTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence conservation of protein binding segments in intrinsically disordered regions.
Biochemical and biophysical research communications494(3-4)602-607(2017 12)
Ota H,Fukuchi S

A metabolic switch controls intestinal differentiation downstream of
eLife6(2017 04)
Sandoval IT,Delacruz RG,Miller BN,Hill S,Olson KA,Gabriel AE,Boyd K,Satterfield C,Remmen HV,Rutter J,Jones DA

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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apc cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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