atp2b1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp2b1a
Entrez Gene ID	378746
Full Name	ATPase plasma membrane Ca2+ transporting 1a
Synonyms	pmca1,pmca1a,sb:cb801,si:dkey-18o7.1,wu:fe47b06,wu:fi41d01
General protein information	Preferred Names ATPase plasma membrane Ca2+ transporting 1a Names plasma membrane calcium-transporting ATPase 1 ATPase, Ca++ transporting, plasma membrane 1a plasma membrane calcium ATPase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021474964.1	XP_021330639.1	plasma membrane calcium-transporting ATPase 1 isoform X1
XM_021474966.1	XP_021330641.1	plasma membrane calcium-transporting ATPase 1 isoform X3
XM_009300256.3	XP_009298531.1	plasma membrane calcium-transporting ATPase 1 isoform X1
NM_001044757.1	NP_001038222.1	plasma membrane calcium-transporting ATPase 1
XM_009300256.2	XP_009298531.1	plasma membrane calcium-transporting ATPase 1 isoform X1
XM_021474965.1	XP_021330640.1	plasma membrane calcium-transporting ATPase 1 isoform X2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ACA4	NP_181687.1
Oryza sativa (rice)	Os01g0939100	NP_001045345.1
Homo sapiens (human)	ATP2B1	NP_001673.2
Gallus gallus (chicken)	ATP2B1	NP_001161474.1
Danio rerio (zebrafish)	atp2b1a	NP_001038222.1
Pan troglodytes (chimpanzee)	ATP2B1	XP_003952314.1
Canis lupus familiaris (dog)	ATP2B1	NP_001184013.1
Bos taurus (cattle)	ATP2B1	NP_777121.1
Arabidopsis thaliana (thale cress)	ACA11	NP_191292.1
Oryza sativa (rice)	Os05g0495600	NP_001055932.2
	ATP2B1	XP_001102031.1
Mus musculus (house mouse)	Atp2b1	NP_080758.1
Rattus norvegicus (Norway rat)	Atp2b1	NP_445763.1
Oryza sativa (rice)	Os12g0136900	NP_001066105.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative analysis of the transcriptome responses of zebrafish embryos after exposure to low concentrations of cadmium, cobalt and copper.
Sonnack L, Klawonn T, Kriehuber R, Hollert H, Sch?fers C, Fenske M
Comparative biochemistry and physiology. Part D, Genomics & proteomics2599-108(2017 Dec)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Biting into the Genome to Phenome Map: Developmental Genetic Modularity of Cichlid Fish Dentitions.
Hulsey CD, Fraser GJ, Meyer A
Integrative and comparative biology56(3)373-88(2016 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

BDNF-TrkB axis regulates migration of the lateral line primordium and modulates the maintenance of mechanoreceptor progenitors.
Gasanov EV, Rafieva LM, Korzh VP
PloS one10(3)e0119711(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Activity of Atp2b1a is required to facilitate calcification of the developing pharyngeal teeth by the dental epithelium.
Title: Plasma membrane Ca(2+) ATPase Atp2b1a regulates bone mineralization in zebrafish.

Atp2b1a functions during progenitor cell proliferation and hair cell differentiation
Title: atp2b1a regulates Ca(2+) export during differentiation and regeneration of mechanosensory hair cells in zebrafish.

Atp2b1a plays an important role for normal development of the auditory and vestibular system as well as calcium transport in the inner ear of zebrafish.
Title: Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear.

The following atp2b1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp2b1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141965	XM_021474966.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa40320	XM_021474965.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa37067	NM_001044757.1 Latest version!	Danio rerio ATPase plasma membrane Ca2+ transporting 1a (atp2b1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40319	XM_009300256.3 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40319	XM_021474964.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa40319	XM_009300256.2	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp2b1a ( XM_021474966.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330641.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa141965
Clone ID Related Accession (Same CDS sequence)	XM_021474966.1
Accession Version	XM_021474966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATGTAACC AATGTTTCTA GCCCTACACA TATCCGGGTG 
GTGAATGCAT TCCGCAGCTC GCTGTCACCC TATGAGGGTC TGGAGAAGCC AGAGTCTCGA 
ACCTCCATCC ACAACTTCAT GACCCATCCA GAATTCCGGA TAGAGGACTC GGAGCCCCAC 
ATCCCTCTCA TCGACGACAC GGACGCGGAG GACGATGCTC CCACAAAGAG AAATGCGACC 
CCTACACCTC CTCCTCCACC CTCACCCAAT CAGAACAATA ATGCCGTCGA TAGCGGCGTG 
CACCTCTTCC TGGATCCCAG CAAATCAGCC ACACCTTCAG CTCCCGGGAG CCCCATGCAC 
AGCCTAGAGA CGTCCCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330641.1
CDS	22..3822
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQMDVVSAFQSGISFQAAVRRQPSSGSQQHDVTNVSSPTHIRVVNAFRSSLSPYEGLEKPESRTSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNATPTPPPPPSPNQNNNAVDSGVHLFLDPSKSATPSAPGSPMHSLETSL

Target ORF information:

RefSeq Version	XM_021474966.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474966.1

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATGTAACC AATGTTTCTA GCCCTACACA TATCCGGGTG 
GTGAATGCAT TCCGCAGCTC GCTGTCACCC TATGAGGGTC TGGAGAAGCC AGAGTCTCGA 
ACCTCCATCC ACAACTTCAT GACCCATCCA GAATTCCGGA TAGAGGACTC GGAGCCCCAC 
ATCCCTCTCA TCGACGACAC GGACGCGGAG GACGATGCTC CCACAAAGAG AAATGCGACC 
CCTACACCTC CTCCTCCACC CTCACCCAAT CAGAACAATA ATGCCGTCGA TAGCGGCGTG 
CACCTCTTCC TGGATCCCAG CAAATCAGCC ACACCTTCAG CTCCCGGGAG CCCCATGCAC 
AGCCTAGAGA CGTCCCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1a ( XM_021474965.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330640.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40320
Clone ID Related Accession (Same CDS sequence)	XM_021474965.1
Accession Version	XM_021474965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATGTAACC AATGTTTCTA GCCCTACACA TGTAGCCTTA 
TCTCCTGCTA CTGCCTTCAA CACCGCTTCT GCCACTGTCG GGTATCCGGG TGGTGAATGC 
ATTCCGCAGC TCGCTGTCAC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330640.1
CDS	368..3934
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQMDVVSAFQSGISFQAAVRRQPSSGSQQHDVTNVSSPTHVALSPATAFNTASATVGYPGGECIPQLAVTL

Target ORF information:

RefSeq Version	XM_021474965.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474965.1

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATGTAACC AATGTTTCTA GCCCTACACA TGTAGCCTTA 
TCTCCTGCTA CTGCCTTCAA CACCGCTTCT GCCACTGTCG GGTATCCGGG TGGTGAATGC 
ATTCCGCAGC TCGCTGTCAC CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1a ( NM_001044757.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038222.1 Danio rerio ATPase plasma membrane Ca2+ transporting 1a (atp2b1a), mRNA.

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CloneID	ODa37067
Clone ID Related Accession (Same CDS sequence)	NM_001044757.1
Accession Version	NM_001044757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649423.7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01001880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATC 
CGGGTGGTGA ATGCATTCCG CAGCTCGCTG TCACCCTATG AGGGTCTGGA GAAGCCAGAG 
TCTCGAACCT CCATCCACAA CTTCATGACC CATCCAGAAT TCCGGATAGA GGACTCGGAG 
CCCCACATCC CTCTCATCGA CGACACGGAC GCGGAGGACG ATGCTCCCAC AAAGAGAAAT 
GCGACCCCTA CACCTCCTCC TCCACCCTCA CCCAATCAGA ACAATAATGC CGTCGATAGC 
GGCGTGCACC TCTTCCTGGA TCCCAGCAAA TCAGCCACAC CTTCAGCTCC CGGGAGCCCC 
ATGCACAGCC TAGAGACGTC CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038222.1
CDS	287..3973
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLSPYEGLEKPESRTSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNATPTPPPPPSPNQNNNAVDSGVHLFLDPSKSATPSAPGSPMHSLETSL

Target ORF information:

RefSeq Version	NM_001044757.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase plasma membrane Ca2+ transporting 1a (atp2b1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044757.1

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATC 
CGGGTGGTGA ATGCATTCCG CAGCTCGCTG TCACCCTATG AGGGTCTGGA GAAGCCAGAG 
TCTCGAACCT CCATCCACAA CTTCATGACC CATCCAGAAT TCCGGATAGA GGACTCGGAG 
CCCCACATCC CTCTCATCGA CGACACGGAC GCGGAGGACG ATGCTCCCAC AAAGAGAAAT 
GCGACCCCTA CACCTCCTCC TCCACCCTCA CCCAATCAGA ACAATAATGC CGTCGATAGC 
GGCGTGCACC TCTTCCTGGA TCCCAGCAAA TCAGCCACAC CTTCAGCTCC CGGGAGCCCC 
ATGCACAGCC TAGAGACGTC CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1a ( XM_021474964.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330639.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40319
Clone ID Related Accession (Same CDS sequence)	XM_021474964.1 , XM_009300256.3 , XM_009300256.2
Accession Version	XM_021474964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330639.1
CDS	368..4141
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQMDVVSAFQSGISFQAAVRRQPSSGSQQHDIRVVNAFRSSLSPYEGLEKPESRTSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNATPTPPPPPSPNQNNNAVDSGVHLFLDPSKSATPSAPGSPMHSLETSL

Target ORF information:

RefSeq Version	XM_021474964.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474964.1

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1a ( XM_009300256.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298531.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40319
Clone ID Related Accession (Same CDS sequence)	XM_021474964.1 , XM_009300256.3 , XM_009300256.2
Accession Version	XM_009300256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298531.1
CDS	862..4635
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQMDVVSAFQSGISFQAAVRRQPSSGSQQHDIRVVNAFRSSLSPYEGLEKPESRTSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNATPTPPPPPSPNQNNNAVDSGVHLFLDPSKSATPSAPGSPMHSLETSL

Target ORF information:

RefSeq Version	XM_009300256.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300256.3

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1a ( XM_009300256.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298531.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.

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CloneID	ODa40319
Clone ID Related Accession (Same CDS sequence)	XM_021474964.1 , XM_009300256.3 , XM_009300256.2
Accession Version	XM_009300256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium-transporting ATPase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298531.1
CDS	793..4566
Translation	MANNSYSGVKNSVAEPNHNGEFGCSLKELRSLMELRGAEGLQKIQETYGDVNGLCNRLKTSAVDGLSGQPSDIEKRKAAFGQNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYKPPDAGDKNCVKGTGGEPEEEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFSVVRGGQVIQIHVAEIVVGDIAQIKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDAMLLSGTHVMEGSGKIVVTAVGVNSQTGIIFTLLGAGEDDDDEEEKEKKKEKERKKEKKNKKQDGSVENRKKAKAQDGAAMEMQPLNSDEGADGEEKRKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVVLFVVDTFWVDKIPWDSGCIPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIADKHYRKVPEPDVVPASTMDLLIVGISVNCAYTTKIMSPEKEGGLNRQVGNKTECALLGFALDLKKDYQAVRNEIPEEKLYKVYTFNSVRKSMSTVLKNDDGSYRMFSKGASEILLKKCFKILTSTGEAKVFRPRDRDDMVKRVIEPMASEGLRTICLAYRDFPASEGEPDWDNEADILTRLTCVCVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNLNTARAIATKCGILQVGDDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLIIIFTLLFAGEKMFNIDSGRYADLHAPPSEHYTIVFNTFVMMQLFNEINARKIHGERNVFEGIFNNMIFCTIVFGTFVIQIVIVQFGGKPFSCVGLNIEQWLWCIFLGFGCLLWGQVISTIPTSRLTFLKTAGHGTQKEEIPDEELEELEDLDEIDHAERELRRGQILWFRGLNRIQTQMDVVSAFQSGISFQAAVRRQPSSGSQQHDIRVVNAFRSSLSPYEGLEKPESRTSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNATPTPPPPPSPNQNNNAVDSGVHLFLDPSKSATPSAPGSPMHSLETSL

Target ORF information:

RefSeq Version	XM_009300256.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1a (atp2b1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300256.2

ORF Insert Sequence:

ATGGCTAACA ACTCATACAG CGGGGTGAAG AACTCTGTGG CCGAGCCCAA TCACAATGGA 
GAGTTTGGCT GCTCGCTTAA AGAGCTGCGC TCTCTTATGG AACTGCGAGG GGCTGAAGGA 
TTACAAAAAA TCCAGGAAAC GTATGGGGAC GTTAATGGAC TTTGCAACAG ACTGAAAACA 
TCGGCAGTAG ATGGTTTAAG TGGGCAGCCC TCAGATATTG AAAAAAGAAA AGCAGCATTT 
GGACAGAACT TTATACCTCC GAAAAAGCCA AAAACCTTTC TTCAGTTAGT ATGGGAAGCA 
TTGCAGGATG TAACATTGAT TATTTTGGAA GTGGCAGCAA TAGTTTCTTT AGGCCTTTCT 
TTTTATAAAC CTCCAGATGC AGGAGATAAA AATTGTGTGA AGGGAACGGG TGGAGAACCT 
GAGGAAGAAG GAGAGACAGG GTGGATAGAA GGTGCAGCTA TCCTGCTTTC TGTTGTTTGT 
GTGGTGCTCG TGACTGCGTT TAATGACTGG AGTAAAGAGA AGCAGTTCCG TGGCTTACAG 
AGCCGCATTG AACAAGAGCA GAAGTTTTCG GTGGTTCGTG GAGGGCAAGT CATTCAGATC 
CATGTGGCCG AGATTGTCGT CGGGGACATT GCACAAATAA AATATGGCGA CCTCCTGCCC 
GCTGACGGCA TTCTCATCCA GGGCAACGAC CTCAAAATAG ACGAGAGCTC TTTAACCGGA 
GAGTCTGATC ATGTCAAGAA ATCATTGGAC AAAGATGCCA TGCTGCTGTC AGGTACACAC 
GTCATGGAGG GCTCTGGAAA AATCGTGGTC ACTGCTGTCG GAGTCAATTC CCAAACTGGA 
ATCATCTTCA CATTATTGGG GGCAGGAGAA GACGACGATG ATGAGGAAGA GAAAGAGAAG 
AAAAAAGAAA AGGAGCGAAA GAAAGAGAAG AAAAATAAAA AGCAGGATGG CTCTGTGGAG 
AACCGAAAGA AAGCAAAAGC CCAGGATGGG GCTGCAATGG AGATGCAGCC TCTAAACAGT 
GATGAAGGGG CTGATGGCGA GGAGAAGAGA AAAGCCAACT TGCCAAAGAA AGAGAAATCG 
GTTTTGCAAG GGAAGCTGAC CAAATTGGCG GTCCAAATCG GAAAAGCAGG TTTGTTGATG 
TCAGCCATCA CCGTGATCAT CTTGGTGGTG CTGTTCGTGG TGGACACTTT CTGGGTGGAT 
AAGATTCCTT GGGATTCAGG ATGTATACCC ATCTACATCC AGTTCTTTGT TAAGTTTTTC 
ATCATTGGTG TGACCGTACT GGTGGTGGCC GTTCCTGAGG GTCTTCCGCT GGCTGTTACC 
ATCTCATTGG CTTACTCTGT CAAAAAAATG ATGAAGGACA ATAATTTAGT GAGGCATCTT 
GACGCCTGCG AAACCATGGG CAATGCCACA GCCATCTGTT CAGATAAGAC AGGAACTCTG 
ACTATGAATC GGATGACAGT AGTTCAGGTT TTTATTGCTG ACAAGCACTA CAGGAAAGTT 
CCTGAGCCTG ACGTTGTCCC TGCCAGCACC ATGGACCTGC TTATAGTGGG CATCAGTGTC 
AACTGTGCTT ACACCACCAA AATTATGTCC CCTGAGAAGG AGGGAGGCCT GAATCGTCAG 
GTGGGCAACA AAACTGAATG TGCCTTGCTG GGCTTTGCCT TGGACTTGAA AAAAGACTAT 
CAAGCAGTAC GCAACGAAAT CCCTGAAGAG AAGCTTTACA AAGTCTACAC CTTCAACTCC 
GTCAGGAAAT CCATGAGCAC CGTGCTAAAG AATGATGACG GAAGCTACCG GATGTTCAGC 
AAAGGAGCCT CAGAGATTCT GCTGAAGAAG TGCTTTAAAA TTCTGACATC GACGGGTGAG 
GCGAAGGTTT TCCGGCCCAG GGACAGAGAT GATATGGTGA AGAGAGTAAT CGAACCTATG 
GCCTCTGAGG GCCTGAGGAC CATCTGCCTG GCTTACAGGG ACTTTCCTGC TTCTGAAGGA 
GAACCAGACT GGGATAATGA GGCCGATATC CTCACCAGGC TCACTTGTGT GTGTGTCGTG 
GGCATTGAAG ATCCGGTTAG GCCTGAGGTC CCTGATGCCA TTAGAAAGTG CCAGCGTGCT 
GGAATCACGG TGCGCATGGT CACAGGGGAC AACCTTAATA CTGCCCGAGC TATAGCAACT 
AAATGTGGCA TCCTGCAGGT AGGAGATGAT TTCCTCTGCT TGGAGGGCAA AGAGTTCAAC 
CGCCGCATCC GTAATGAAAA GGGAGAGATC GAACAAGAGC GGATAGACAA AATATGGCCT 
AAACTCCGGG TCCTGGCGAG ATCTTCGCCC ACAGACAAGC ATACACTAGT TAAAGGAATA 
ATTGACAGCA CTGTTGTGGA GCAGAGACAG GTTGTAGCAG TAACAGGAGA CGGCACCAAT 
GATGGCCCTG CCTTAAAAAA GGCCGATGTT GGGTTTGCCA TGGGTATAGC CGGCACTGAC 
GTGGCTAAAG AAGCCTCCGA CATTATCCTC ACTGATGACA ACTTTAGCAG CATTGTCAAG 
GCCGTCATGT GGGGGCGAAA TGTCTACGAC AGCATTTCAA AATTCCTCCA GTTCCAGCTC 
ACCGTCAACG TGGTGGCTGT GATTGTGGCC TTCACTGGAG CTTGCATCAC ACAGGACTCT 
CCTTTAAAAG CTGTACAGAT GTTATGGGTA AATCTCATTA TGGACACACT TGCCTCTTTG 
GCCTTGGCCA CTGAACCCCC CACTGAATCT CTTCTGCTGA GGAAGCCTTA TGGCCGTAAT 
AAACCGCTCA TCTCTAGGAC AATGATGAAA AACATATTAG GTCATGCTGT CTACCAGCTC 
ATCATCATCT TTACTCTACT GTTTGCTGGT GAGAAGATGT TCAACATTGA CAGTGGACGT 
TACGCCGATC TTCACGCTCC CCCATCTGAG CATTACACGA TCGTCTTCAA CACCTTTGTG 
ATGATGCAGC TGTTCAATGA AATTAATGCA CGGAAGATCC ACGGAGAACG AAACGTCTTT 
GAAGGCATTT TCAACAATAT GATCTTCTGC ACCATTGTCT TTGGGACATT CGTTATTCAG 
ATTGTGATCG TGCAGTTTGG AGGAAAGCCG TTCAGCTGTG TTGGTTTAAA CATTGAACAG 
TGGCTATGGT GCATCTTCTT GGGCTTTGGA TGTCTACTCT GGGGACAGGT CATCTCCACG 
ATCCCCACAA GCCGGCTGAC GTTCCTGAAG ACTGCTGGTC ATGGAACGCA GAAGGAGGAA 
ATCCCGGATG AGGAGTTGGA AGAGCTGGAA GACCTTGATG AAATTGACCA CGCCGAGCGA 
GAGCTCCGCA GGGGGCAGAT CCTCTGGTTT AGAGGCCTCA ACCGCATTCA GACCCAGATG 
GATGTAGTGA GTGCGTTCCA GAGCGGAATT TCCTTTCAGG CGGCCGTCAG GCGCCAGCCC 
TCCAGCGGCA GCCAACAGCA CGATATCCGG GTGGTGAATG CATTCCGCAG CTCGCTGTCA 
CCCTATGAGG GTCTGGAGAA GCCAGAGTCT CGAACCTCCA TCCACAACTT CATGACCCAT 
CCAGAATTCC GGATAGAGGA CTCGGAGCCC CACATCCCTC TCATCGACGA CACGGACGCG 
GAGGACGATG CTCCCACAAA GAGAAATGCG ACCCCTACAC CTCCTCCTCC ACCCTCACCC 
AATCAGAACA ATAATGCCGT CGATAGCGGC GTGCACCTCT TCCTGGATCC CAGCAAATCA 
GCCACACCTT CAGCTCCCGG GAGCCCCATG CACAGCCTAG AGACGTCCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative analysis of the transcriptome responses of zebrafish embryos after exposure to low concentrations of cadmium, cobalt and copper.
Comparative biochemistry and physiology. Part D, Genomics & proteomics2599-108(2017 Dec)
Sonnack L,Klawonn T,Kriehuber R,Hollert H,Sch?fers C,Fenske M

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Biting into the Genome to Phenome Map: Developmental Genetic Modularity of Cichlid Fish Dentitions.
Integrative and comparative biology56(3)373-88(2016 Sep)
Hulsey CD,Fraser GJ,Meyer A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

BDNF-TrkB axis regulates migration of the lateral line primordium and modulates the maintenance of mechanoreceptor progenitors.
PloS one10(3)e0119711(2015)
Gasanov EV,Rafieva LM,Korzh VP

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atp2b1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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