selenow2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenow2b
Entrez Gene ID	378438
Full Name	selenoprotein W, 2b
Synonyms	cb696,sepw2b,si:dz180g5.2,zgc:123142,zgc:152839
General protein information	Preferred Names selenoprotein W, 2b Names selenoprotein W2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Unlike in mammals, where this protein is highly expressed in skeletal muscle, heart and brain; it is expressed in sensory organs in zebrafish. Paralogs of this gene, sepw1 and sepw2a, are found on chromosomes 5 and 3, respectively. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_194418.2	NP_919399.3	selenoprotein W, 2b
NM_194418.3	NP_919399.3	selenoprotein W2b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MIEN1	XP_005624609.1
Gallus gallus (chicken)	MIEN1	XP_003642854.2
Danio rerio (zebrafish)	sepw2a	NP_919398.1
Homo sapiens (human)	MIEN1	NP_115715.3
Pan troglodytes (chimpanzee)	MIEN1	NP_001230776.1
	MIEN1	XP_001091012.1
Bos taurus (cattle)	MIEN1	NP_001068689.1
Mus musculus (house mouse)	Mien1	NP_079835.1
Xenopus tropicalis (tropical clawed frog)	mien1	NP_001015996.1
Danio rerio (zebrafish)	sepw2b	NP_919399.3

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Toxicogenomic analysis in the combined effect of tributyltin and benzo[a]pyrene on the development of zebrafish embryos.
Huang L, Zuo Z, Zhang Y, Wang C
Aquatic toxicology (Amsterdam, Netherlands)158157-64(2015 Jan)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenow2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenow2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36765	NM_194418.3 Latest version!	Danio rerio selenoprotein W, 2b (selenow2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa36765	NM_194418.2	Danio rerio selenoprotein W, 2b (sepw2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenow2b ( NM_194418.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919399.3 Danio rerio selenoprotein W, 2b (sepw2b), mRNA.

Price & Availability↑

CloneID	ODa36765
Clone ID Related Accession (Same CDS sequence)	NM_194418.2 , NM_194418.3
Accession Version	NM_194418.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(Zebrafish)
Product	selenoprotein W, 2b
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV591466.1, CK687346.1, EH115335.1, EE204645.1 and AW232613.1. On Sep 20, 2007 this sequence version replaced NM_194418.1. ##Evidence-Data-START## Transcript exon combination :: BC124148.1, BC107829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCGTGA AGGTCAAAAT CGAATACTGT GGTGCATGAG GTTACGAGCC TCGCTTCCAG 
GAGCTGAAGC GAGAAATCTG TGGAAACTGT CCTGATGCTG AGGTGTCCGG CTTCGTGGGC 
AGGAGAGGAT GCTTTGAGAT ACAGATCAAT GACTTTTTGG TTTTCTCAAA GCTGGAGTCA 
GGTGGTTTCC CTTACAGTGA GGATATTATA GAGGCCGTTG TTAAGGCAAA GGATGGGAAG 
CCTGAAAAGA TCACCAGGAA CCGTAAAGAG TGCATCATCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919399.3
CDS	118..402
Translation	MVVKVKIEYCGA*GYEPRFQELKREICGNCPDAEVSGFVGRRGCFEIQINDFLVFSKLESGGFPYSEDIIEAVVKAKDGKPEKITRNRKECIIL

Target ORF information:

RefSeq Version	NM_194418.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio selenoprotein W, 2b (sepw2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194418.2

ORF Insert Sequence:

ATGGTCGTGA AGGTCAAAAT CGAATACTGT GGTGCATGAG GTTACGAGCC TCGCTTCCAG 
GAGCTGAAGC GAGAAATCTG TGGAAACTGT CCTGATGCTG AGGTGTCCGG CTTCGTGGGC 
AGGAGAGGAT GCTTTGAGAT ACAGATCAAT GACTTTTTGG TTTTCTCAAA GCTGGAGTCA 
GGTGGTTTCC CTTACAGTGA GGATATTATA GAGGCCGTTG TTAAGGCAAA GGATGGGAAG 
CCTGAAAAGA TCACCAGGAA CCGTAAAGAG TGCATCATCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

selenow2b ( NM_194418.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919399.3 Danio rerio selenoprotein W, 2b (selenow2b), mRNA.

Price & Availability↑

CloneID	ODa36765
Clone ID Related Accession (Same CDS sequence)	NM_194418.2 , NM_194418.3
Accession Version	NM_194418.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Danio rerio(zebrafish)
Product	selenoprotein W2b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV591466.1, CK687346.1, EH468996.1 and BC124148.1. On May 11, 2017 this sequence version replaced NM_194418.2. ##Evidence-Data-START## Transcript exon combination :: BC124148.1, GDQH01020999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476768, SAMEA4476773 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCGTGA AGGTCAAAAT CGAATACTGT GGTGCATGAG GTTACGAGCC TCGCTTCCAG 
GAGCTGAAGC GAGAAATCTG TGGAAACTGT CCTGATGCTG AGGTGTCCGG CTTCGTGGGC 
AGGAGAGGAT GCTTTGAGAT ACAGATCAAT GACTTTTTGG TTTTCTCAAA GCTGGAGTCA 
GGTGGTTTCC CTTACAGTGA GGATATTATA GAGGCCGTTG TTAAGGCAAA GGATGGGAAG 
CCTGAAAAGA TCACCAGGAA CCGTAAAGAG TGCATCATCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919399.3
CDS	127..411
Translation	MVVKVKIEYCGA*GYEPRFQELKREICGNCPDAEVSGFVGRRGCFEIQINDFLVFSKLESGGFPYSEDIIEAVVKAKDGKPEKITRNRKECIIL

Target ORF information:

RefSeq Version	NM_194418.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein W, 2b (selenow2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194418.3

ORF Insert Sequence:

ATGGTCGTGA AGGTCAAAAT CGAATACTGT GGTGCATGAG GTTACGAGCC TCGCTTCCAG 
GAGCTGAAGC GAGAAATCTG TGGAAACTGT CCTGATGCTG AGGTGTCCGG CTTCGTGGGC 
AGGAGAGGAT GCTTTGAGAT ACAGATCAAT GACTTTTTGG TTTTCTCAAA GCTGGAGTCA 
GGTGGTTTCC CTTACAGTGA GGATATTATA GAGGCCGTTG TTAAGGCAAA GGATGGGAAG 
CCTGAAAAGA TCACCAGGAA CCGTAAAGAG TGCATCATCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Toxicogenomic analysis in the combined effect of tributyltin and benzo[a]pyrene on the development of zebrafish embryos.
Aquatic toxicology (Amsterdam, Netherlands)158157-64(2015 Jan)
Huang L,Zuo Z,Zhang Y,Wang C

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

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selenow2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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