selenow2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenow2a
Entrez Gene ID	378437
Full Name	selenoprotein W, 2a
Synonyms	cb695,sepw2a,si:busm1-180g5.9,si:dz180g5.9
General protein information	Preferred Names selenoprotein W, 2a Names selenoprotein W2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Unlike in mammals, where this protein is highly expressed in skeletal muscle, heart and brain; it is expressed in sensory organs in zebrafish. Paralogs of this gene, sepw1 and sepw2b, are found on chromosomes 5 and 3, respectively. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_194417.3	NP_919398.1	selenoprotein W2a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MIEN1	XP_005624609.1
Gallus gallus (chicken)	MIEN1	XP_003642854.2
Danio rerio (zebrafish)	sepw2a	NP_919398.1
Homo sapiens (human)	MIEN1	NP_115715.3
Pan troglodytes (chimpanzee)	MIEN1	NP_001230776.1
	MIEN1	XP_001091012.1
Bos taurus (cattle)	MIEN1	NP_001068689.1
Mus musculus (house mouse)	Mien1	NP_079835.1
Xenopus tropicalis (tropical clawed frog)	mien1	NP_001015996.1
Danio rerio (zebrafish)	sepw2b	NP_919399.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenow2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenow2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36764	NM_194417.3 Latest version!	Danio rerio selenoprotein W, 2a (selenow2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenow2a ( NM_194417.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919398.1 Danio rerio selenoprotein W, 2a (selenow2a), mRNA.

Price & Availability↑

CloneID	ODa36764
Clone ID Related Accession (Same CDS sequence)	NM_194417.3
Accession Version	NM_194417.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	selenoprotein W2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EB966620.1, CK683291.1, BC162538.1, CK686601.1, EB842527.1, CT573444.4, EH998455.1, EB939815.1, EB944462.1, EB766662.1 and BF718050.1. On Feb 4, 2010 this sequence version replaced NM_194417.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01034847.1, AY221261.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGTAC AAATAAAAGT TGAATATTGT GGTGGATGAG GGTACGAGCC CCGCTATCAG 
GAGCTCAAGC GGGTCGTCAC TGCTGAGTTC ACTGATGCGG ATGTGAGTGG CTTCGTCGGC 
CGCCAAGGGA GCTTTGAGAT CGAGATCAAT GGACAGCTGA TTTTCTCAAA GCTGGAAACC 
AGTGGCTTCC CCTATGAAGA TGATATCATG GGTGTAATCC AAAGAGCCTA TGATGGCCAG 
CCAGTGGAGA AGATCACCAA GAGCCAACCA CCCTGTGTCA TCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919398.1
CDS	59..346
Translation	MGVQIKVEYCGG*GYEPRYQELKRVVTAEFTDADVSGFVGRQGSFEIEINGQLIFSKLETSGFPYEDDIMGVIQRAYDGQPVEKITKSQPPCVIL

Target ORF information:

RefSeq Version	NM_194417.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein W, 2a (selenow2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194417.3

ORF Insert Sequence:

ATGGGTGTAC AAATAAAAGT TGAATATTGT GGTGGATGAG GGTACGAGCC CCGCTATCAG 
GAGCTCAAGC GGGTCGTCAC TGCTGAGTTC ACTGATGCGG ATGTGAGTGG CTTCGTCGGC 
CGCCAAGGGA GCTTTGAGAT CGAGATCAAT GGACAGCTGA TTTTCTCAAA GCTGGAAACC 
AGTGGCTTCC CCTATGAAGA TGATATCATG GGTGTAATCC AAAGAGCCTA TGATGGCCAG 
CCAGTGGAGA AGATCACCAA GAGCCAACCA CCCTGTGTCA TCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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selenow2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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