ORF » Species Summary » Danio rerio » pak1 cDNA ORF clone

pak1 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following pak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa02976 NM_201328.1
Latest version!		 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $342.30
$489.00
ODa149827 XM_021467598.1
Latest version!		 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
ODa149828 XM_021467599.1
Latest version!		 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pak1 ( NM_201328.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_958485.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), mRNA.
    Price & Availability↑
    CloneID ODa02976
    Clone ID Related Accession (Same CDS sequence) NM_201328.1
    Accession Version NM_201328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-08
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase PAK 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044138.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCC GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AAAACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACCACCAA CACCACAAAC ACCACCAACA CCACCTCCAC CAGCGCCGCC 
    TCCAGCACCA ACGCTCCATC CACTCCACAG ACCACAGACA AGAGCCGCAA GAAGAAGATG 
    AGCGACGAGG AGATTCTGGA GAAACTCAGG AGTATAGTCA GCGTCGGCGA CCCGAAAAAG 
    AAGTACACGC GCTTTGAGAA GATCGGCCAG GGGGCATCTG GAACCGTCTA TACAGCCATA 
    GACATCGCTA CAGGACAAGA GGTGGCCATC AAACAGATGA ACCTGCAGCA GCAGCCCAAG 
    AAAGAGCTGA TCATCAACGA GATCCTGGTC ATGAGGGAAA ACAAGAACCC AAACATCGTC 
    AACTACCTGG ACAGTTATCT GGTTGGCGAT GAGCTGTGGG TGGTGATGGA GTATCTGGCT 
    GGAGGCTCGC TGACGGATGT GGTGACAGAA ACCTGTATGG ATGAAGCACA GATCGCAGCC 
    GTCTGTCGGG AGTGTCTGCA GGCTCTGGAG TTCCTGCACT GCAATCAGGT GATTCACAGA 
    GACATCAAGA GCGACAACAT CCTGCTGGGG ATGGACGGCT CCGTCAAACT CACTGATTTC 
    GGCTTCTGCG CTCAGATCAC TCCCGAGCAG AGCAAGCGCA GCACAATGGT CGGGACGCCC 
    TACTGGATGG CGCCGGAGGT CGTGACCCGC AAGGCTTACG GGCCCAAAGT GGACATCTGG 
    TCTCTGGGCA TCATGGCCAT CGAGATGATC GAGGGCGAAC CGCCCTACCT CAACGAGAAC 
    CCGCTCCGGG CTCTGTATCT GATCGCCACT AATGGGACGC CGGAGCTGCA GAACCCAGAG 
    AAACTTTCTG CTGTATTTCG GGATTTTCTA AACCGCTGTT TGGAGATGGA CGTGGAGAAA 
    AGAGGCTCTG CCAAAGAGCT CCTACAGCAC CAGTTCCTGA AGGTGGCCAA GCCTCTGTCC 
    AGCCTCACGC CCCTGATCAT AGCGGCCAAA GAGGCAGCAA AAAACAACCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958485.1
    CDS123..1856
    Translation

    Target ORF information:

    RefSeq Version NM_201328.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_201328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCC GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AAAACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACCACCAA CACCACAAAC ACCACCAACA CCACCTCCAC CAGCGCCGCC 
    TCCAGCACCA ACGCTCCATC CACTCCACAG ACCACAGACA AGAGCCGCAA GAAGAAGATG 
    AGCGACGAGG AGATTCTGGA GAAACTCAGG AGTATAGTCA GCGTCGGCGA CCCGAAAAAG 
    AAGTACACGC GCTTTGAGAA GATCGGCCAG GGGGCATCTG GAACCGTCTA TACAGCCATA 
    GACATCGCTA CAGGACAAGA GGTGGCCATC AAACAGATGA ACCTGCAGCA GCAGCCCAAG 
    AAAGAGCTGA TCATCAACGA GATCCTGGTC ATGAGGGAAA ACAAGAACCC AAACATCGTC 
    AACTACCTGG ACAGTTATCT GGTTGGCGAT GAGCTGTGGG TGGTGATGGA GTATCTGGCT 
    GGAGGCTCGC TGACGGATGT GGTGACAGAA ACCTGTATGG ATGAAGCACA GATCGCAGCC 
    GTCTGTCGGG AGTGTCTGCA GGCTCTGGAG TTCCTGCACT GCAATCAGGT GATTCACAGA 
    GACATCAAGA GCGACAACAT CCTGCTGGGG ATGGACGGCT CCGTCAAACT CACTGATTTC 
    GGCTTCTGCG CTCAGATCAC TCCCGAGCAG AGCAAGCGCA GCACAATGGT CGGGACGCCC 
    TACTGGATGG CGCCGGAGGT CGTGACCCGC AAGGCTTACG GGCCCAAAGT GGACATCTGG 
    TCTCTGGGCA TCATGGCCAT CGAGATGATC GAGGGCGAAC CGCCCTACCT CAACGAGAAC 
    CCGCTCCGGG CTCTGTATCT GATCGCCACT AATGGGACGC CGGAGCTGCA GAACCCAGAG 
    AAACTTTCTG CTGTATTTCG GGATTTTCTA AACCGCTGTT TGGAGATGGA CGTGGAGAAA 
    AGAGGCTCTG CCAAAGAGCT CCTACAGCAC CAGTTCCTGA AGGTGGCCAA GCCTCTGTCC 
    AGCCTCACGC CCCTGATCAT AGCGGCCAAA GAGGCAGCAA AAAACAACCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pak1 ( XM_021467598.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323273.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa149827
    Clone ID Related Accession (Same CDS sequence) XM_021467598.1
    Accession Version XM_021467598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase PAK 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCA GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AACACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACAAACAC CACCAACACC ACAAACACCA CCAACACCAC CTCCACCAGC 
    GCCGCCTCCA GCACCAACGC TCCATCCACT CCACAGACCA CAGACAAGAG CCGCAAGAAG 
    AAGATGAGCG ACGAGGAGAT TCTGGAGAAA CTCAACGTGC TCCGGTCTCC CTCTGCTGGG 
    AGTATAGTCA GCGTCGGCGA CCCGAAAAAG AAGTACACGC GCTTTGAGAA GATCGGCCAG 
    GGGGCATCTG GAACCGTCTA TACAGCCATA GACATCGCTA CAGGACAAGA GGTGGCCATC 
    AAACAGATGA ACCTGCAGCA GCAGCCCAAG AAAGAGCTGA TCATCAACGA GATCCTGGTC 
    ATGAGGGAAA ACAAGAACCC AAACATCGTC AACTACCTGG ACAGTTATCT GGTTGGCGAT 
    GAGCTGTGGG TGGTGATGGA GTATCTGGCT GGAGGCTCGC TGACGGATGT GGTGACAGAA 
    ACCTGTATGG ATGAAGCACA GATCGCAGCC GTCTGTCGGG AGTGTCTGCA GGCTCTGGAG 
    TTCCTGCACT GCAATCAGGT GATTCACAGA GACATCAAGA GCGACAACAT CCTGCTGGGG 
    ATGGACGGCT CCGTCAAACT CACTGATTTC GGCTTCTGCG CTCAGATCAC TCCCGAGCAG 
    AGCAAGCGCA GCACAATGGT CGGGACGCCC TACTGGATGG CGCCGGAGGT GGTGACCCGC 
    AAGGCTTACG GGCCCAAAGT GGACATCTGG TCTCTGGGCA TCATGGCCAT CGAGATGATC 
    GAGGGCGAAC CGCCCTACCT CAACGAGAAC CCACTCCGGG CTCTGTATCT GATCGCCACT 
    AATGGGACGC CGGAGCTGCA GAACCCAGAG AAGCTTTCTG CTGTATTTCG GGATTTTCTA 
    AACCGCTGTT TGGAGATGGA CGTGGAGAAA AGAGGCTCTG CCAAAGAGCT CCTACAGCAC 
    CAGTTCCTGA AGGTGGCCAA GCCTCTGTCC AGCCTCACGC CCCTGATCAT AGCGGCCAAA 
    GAGGCAGCAA AAAACAACCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323273.1
    CDS46..1809
    Translation

    Target ORF information:

    RefSeq Version XM_021467598.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021467598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCA GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AACACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACAAACAC CACCAACACC ACAAACACCA CCAACACCAC CTCCACCAGC 
    GCCGCCTCCA GCACCAACGC TCCATCCACT CCACAGACCA CAGACAAGAG CCGCAAGAAG 
    AAGATGAGCG ACGAGGAGAT TCTGGAGAAA CTCAACGTGC TCCGGTCTCC CTCTGCTGGG 
    AGTATAGTCA GCGTCGGCGA CCCGAAAAAG AAGTACACGC GCTTTGAGAA GATCGGCCAG 
    GGGGCATCTG GAACCGTCTA TACAGCCATA GACATCGCTA CAGGACAAGA GGTGGCCATC 
    AAACAGATGA ACCTGCAGCA GCAGCCCAAG AAAGAGCTGA TCATCAACGA GATCCTGGTC 
    ATGAGGGAAA ACAAGAACCC AAACATCGTC AACTACCTGG ACAGTTATCT GGTTGGCGAT 
    GAGCTGTGGG TGGTGATGGA GTATCTGGCT GGAGGCTCGC TGACGGATGT GGTGACAGAA 
    ACCTGTATGG ATGAAGCACA GATCGCAGCC GTCTGTCGGG AGTGTCTGCA GGCTCTGGAG 
    TTCCTGCACT GCAATCAGGT GATTCACAGA GACATCAAGA GCGACAACAT CCTGCTGGGG 
    ATGGACGGCT CCGTCAAACT CACTGATTTC GGCTTCTGCG CTCAGATCAC TCCCGAGCAG 
    AGCAAGCGCA GCACAATGGT CGGGACGCCC TACTGGATGG CGCCGGAGGT GGTGACCCGC 
    AAGGCTTACG GGCCCAAAGT GGACATCTGG TCTCTGGGCA TCATGGCCAT CGAGATGATC 
    GAGGGCGAAC CGCCCTACCT CAACGAGAAC CCACTCCGGG CTCTGTATCT GATCGCCACT 
    AATGGGACGC CGGAGCTGCA GAACCCAGAG AAGCTTTCTG CTGTATTTCG GGATTTTCTA 
    AACCGCTGTT TGGAGATGGA CGTGGAGAAA AGAGGCTCTG CCAAAGAGCT CCTACAGCAC 
    CAGTTCCTGA AGGTGGCCAA GCCTCTGTCC AGCCTCACGC CCCTGATCAT AGCGGCCAAA 
    GAGGCAGCAA AAAACAACCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pak1 ( XM_021467599.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323274.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa149828
    Clone ID Related Accession (Same CDS sequence) XM_021467599.1
    Accession Version XM_021467599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase PAK 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCA GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AACACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACAAACAC CACCAACACC ACAAACACCA CCAACACCAC CTCCACCAGC 
    GCCGCCTCCA GCACCAACGC TCCATCCACT CCACAGACCA CAGACAAGAG CCGCAAGAAG 
    AAGATGAGCG ACGAGGAGAT TCTGGAGAAA CTCAGGAGTA TAGTCAGCGT CGGCGACCCG 
    AAAAAGAAGT ACACGCGCTT TGAGAAGATC GGCCAGGGGG CATCTGGAAC CGTCTATACA 
    GCCATAGACA TCGCTACAGG ACAAGAGGTG GCCATCAAAC AGATGAACCT GCAGCAGCAG 
    CCCAAGAAAG AGCTGATCAT CAACGAGATC CTGGTCATGA GGGAAAACAA GAACCCAAAC 
    ATCGTCAACT ACCTGGACAG TTATCTGGTT GGCGATGAGC TGTGGGTGGT GATGGAGTAT 
    CTGGCTGGAG GCTCGCTGAC GGATGTGGTG ACAGAAACCT GTATGGATGA AGCACAGATC 
    GCAGCCGTCT GTCGGGAGTG TCTGCAGGCT CTGGAGTTCC TGCACTGCAA TCAGGTGATT 
    CACAGAGACA TCAAGAGCGA CAACATCCTG CTGGGGATGG ACGGCTCCGT CAAACTCACT 
    GATTTCGGCT TCTGCGCTCA GATCACTCCC GAGCAGAGCA AGCGCAGCAC AATGGTCGGG 
    ACGCCCTACT GGATGGCGCC GGAGGTGGTG ACCCGCAAGG CTTACGGGCC CAAAGTGGAC 
    ATCTGGTCTC TGGGCATCAT GGCCATCGAG ATGATCGAGG GCGAACCGCC CTACCTCAAC 
    GAGAACCCAC TCCGGGCTCT GTATCTGATC GCCACTAATG GGACGCCGGA GCTGCAGAAC 
    CCAGAGAAGC TTTCTGCTGT ATTTCGGGAT TTTCTAAACC GCTGTTTGGA GATGGACGTG 
    GAGAAAAGAG GCTCTGCCAA AGAGCTCCTA CAGCACCAGT TCCTGAAGGT GGCCAAGCCT 
    CTGTCCAGCC TCACGCCCCT GATCATAGCG GCCAAAGAGG CAGCAAAAAA CAACCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323274.1
    CDS45..1784
    Translation

    Target ORF information:

    RefSeq Version XM_021467599.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio p21 protein (Cdc42/Rac)-activated kinase 1 (pak1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021467599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCAGACA ATGGGGAAGT AGAGGACAAG CCGCCAGCTC CGCCCATGAG GAACACCAGC 
    ACTATGATTG GCTCGTCCGG TAAAGACTCA GGACCGCTGA ATCACGGCTC CAAACCGCTG 
    CCGCCCAATC CAGAGGACAA GAGGAGGAAA GAGGGATTCA TACGATCCAT CCTGACCGGA 
    GGAGGAGACA AGACCAATAA GAAAAAGGAG CGTCCAGAGA TCTCCCTGCC ATCAGACTTT 
    GAGCACACCA TCCATGTTGG CTTTGACGCA GTCACTGGAG AGTTCACTGG CATGCCTGAG 
    CAGTGGGCTC GATTACTCCA GACGTCCAAC ATCACCAAAC TGGAGCAGAA GAAGAATCCT 
    CAGGCCGTTC TAGACGTGCT CAAATTCTAC GACTCGAAAG AGACGTCCAA CAACCAGAAA 
    TACATGAGCT TTACAGATAA AGGTGTGGAG AACTTCAACT CCTCCAGCGC AGTGGTCCCC 
    AAAACTGTGT CTGAAACGCC CGCCGTCGCC ACCGTCTCCG AGGATGAGGA CGAGGAAGAG 
    GATGAAGCCA CGCCGCCACC CGTCATCGCC CCCCGGCCAG AACACACCAA ATCTATCTAC 
    ACCCGCTCCG TCATCGAGCC TCTTCCTCCT CCGCTCTCCA CCAAAGACGC CGCAACTTCA 
    CCGATCACGA ACGCAGCCGC AGACGGAGGA TCATCCAGCA ATGCGCCTTC ATCAAACACA 
    AACACCACAA ACACAAACAC CACCAACACC ACAAACACCA CCAACACCAC CTCCACCAGC 
    GCCGCCTCCA GCACCAACGC TCCATCCACT CCACAGACCA CAGACAAGAG CCGCAAGAAG 
    AAGATGAGCG ACGAGGAGAT TCTGGAGAAA CTCAGGAGTA TAGTCAGCGT CGGCGACCCG 
    AAAAAGAAGT ACACGCGCTT TGAGAAGATC GGCCAGGGGG CATCTGGAAC CGTCTATACA 
    GCCATAGACA TCGCTACAGG ACAAGAGGTG GCCATCAAAC AGATGAACCT GCAGCAGCAG 
    CCCAAGAAAG AGCTGATCAT CAACGAGATC CTGGTCATGA GGGAAAACAA GAACCCAAAC 
    ATCGTCAACT ACCTGGACAG TTATCTGGTT GGCGATGAGC TGTGGGTGGT GATGGAGTAT 
    CTGGCTGGAG GCTCGCTGAC GGATGTGGTG ACAGAAACCT GTATGGATGA AGCACAGATC 
    GCAGCCGTCT GTCGGGAGTG TCTGCAGGCT CTGGAGTTCC TGCACTGCAA TCAGGTGATT 
    CACAGAGACA TCAAGAGCGA CAACATCCTG CTGGGGATGG ACGGCTCCGT CAAACTCACT 
    GATTTCGGCT TCTGCGCTCA GATCACTCCC GAGCAGAGCA AGCGCAGCAC AATGGTCGGG 
    ACGCCCTACT GGATGGCGCC GGAGGTGGTG ACCCGCAAGG CTTACGGGCC CAAAGTGGAC 
    ATCTGGTCTC TGGGCATCAT GGCCATCGAG ATGATCGAGG GCGAACCGCC CTACCTCAAC 
    GAGAACCCAC TCCGGGCTCT GTATCTGATC GCCACTAATG GGACGCCGGA GCTGCAGAAC 
    CCAGAGAAGC TTTCTGCTGT ATTTCGGGAT TTTCTAAACC GCTGTTTGGA GATGGACGTG 
    GAGAAAAGAG GCTCTGCCAA AGAGCTCCTA CAGCACCAGT TCCTGAAGGT GGCCAAGCCT 
    CTGTCCAGCC TCACGCCCCT GATCATAGCG GCCAAAGAGG CAGCAAAAAA CAACCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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