kif1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif1b
Entrez Gene ID	369190
Full Name	kinesin family member 1B
Synonyms	unm_st43,wu:fb74c11
General protein information	Preferred Names kinesin family member 1B Names kinesin-like protein KIF1B unm st43
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296815.3	XP_009295090.1	kinesin-like protein KIF1B isoform X5
XM_009296820.3	XP_009295095.1	kinesin-like protein KIF1B isoform X9
XM_009296825.3	XP_009295100.1	kinesin-like protein KIF1B isoform X16
XM_009296829.3	XP_009295104.1	kinesin-like protein KIF1B isoform X20
XM_009296832.3	XP_009295107.1	kinesin-like protein KIF1B isoform X23
XM_009296813.2	XP_009295088.1	kinesin-like protein KIF1B isoform X2
XM_009296815.2	XP_009295090.1	kinesin-like protein KIF1B isoform X4
XM_009296817.2	XP_009295092.1	kinesin-like protein KIF1B isoform X6
XM_009296820.2	XP_009295095.1	kinesin-like protein KIF1B isoform X8
XM_009296821.2	XP_009295096.1	kinesin-like protein KIF1B isoform X9
XM_017353527.1	XP_017209016.1	kinesin-like protein KIF1B isoform X12
XM_009296826.2	XP_009295101.1	kinesin-like protein KIF1B isoform X15
XM_009296827.2	XP_009295102.1	kinesin-like protein KIF1B isoform X16
XM_009296832.2	XP_009295107.1	kinesin-like protein KIF1B isoform X22
XM_009296822.2	XP_009295097.1	kinesin-like protein KIF1B isoform X10
XM_009296823.2	XP_009295098.1	kinesin-like protein KIF1B isoform X11
XM_009296829.2	XP_009295104.1	kinesin-like protein KIF1B isoform X18
XM_009296830.2	XP_009295105.1	kinesin-like protein KIF1B isoform X19
XM_009296813.3	XP_009295088.1	kinesin-like protein KIF1B isoform X2
XM_009296814.3	XP_009295089.1	kinesin-like protein KIF1B isoform X3
XM_021469758.1	XP_021325433.1	kinesin-like protein KIF1B isoform X4
XM_009296817.3	XP_009295092.1	kinesin-like protein KIF1B isoform X7
XM_009296828.3	XP_009295103.1	kinesin-like protein KIF1B isoform X19
XM_009296831.3	XP_009295106.1	kinesin-like protein KIF1B isoform X21
XM_009296812.2	XP_009295087.1	kinesin-like protein KIF1B isoform X1
XM_009296814.2	XP_009295089.1	kinesin-like protein KIF1B isoform X3
XM_009296824.2	XP_009295099.1	kinesin-like protein KIF1B isoform X13
XM_009296816.3	XP_009295091.1	kinesin-like protein KIF1B isoform X6
XM_009296818.3	XP_009295093.1	kinesin-like protein KIF1B isoform X8
XM_009296821.3	XP_009295096.1	kinesin-like protein KIF1B isoform X10
XM_009296824.3	XP_009295099.1	kinesin-like protein KIF1B isoform X15
XM_005162237.4	XP_005162294.1	kinesin-like protein KIF1B isoform X22
XM_009296818.2	XP_009295093.1	kinesin-like protein KIF1B isoform X7
XM_009296831.2	XP_009295106.1	kinesin-like protein KIF1B isoform X20
XM_005162237.3	XP_005162294.1	kinesin-like protein KIF1B isoform X21
XM_009296812.3	XP_009295087.1	kinesin-like protein KIF1B isoform X1
XM_009296822.3	XP_009295097.1	kinesin-like protein KIF1B isoform X11
XM_021469759.1	XP_021325434.1	kinesin-like protein KIF1B isoform X14
XM_009296826.3	XP_009295101.1	kinesin-like protein KIF1B isoform X17
XM_009296827.3	XP_009295102.1	kinesin-like protein KIF1B isoform X18
NM_194382.3	NP_919363.3	kinesin-like protein KIF1B
XM_009296816.2	XP_009295091.1	kinesin-like protein KIF1B isoform X5
XM_009296825.2	XP_009295100.1	kinesin-like protein KIF1B isoform X14
XM_009296828.2	XP_009295103.1	kinesin-like protein KIF1B isoform X17
XM_009296823.3	XP_009295098.1	kinesin-like protein KIF1B isoform X12
XM_017353527.2	XP_017209016.1	kinesin-like protein KIF1B isoform X13

Pathways from BioSystems

Homologies

	LOC722674	XP_002808317.1
Gallus gallus (chicken)	KIF1B	XP_004947511.1
Rattus norvegicus (Norway rat)	Kif1b	NP_476548.1
Canis lupus familiaris (dog)	KIF1B	XP_536743.3
Mus musculus (house mouse)	Kif1b	NP_997565.2
Danio rerio (zebrafish)	kif1b	NP_919363.2
Homo sapiens (human)	KIF1B	NP_055889.2
Bos taurus (cattle)	KIF1B	XP_005217049.1
Xenopus tropicalis (tropical clawed frog)	kif1b	XP_002939603.2

Gene Ontology
Bibliography

Related articles in PubMed

Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones.
Drerup CM, Lusk S, Nechiporuk A
The Journal of neuroscience : the official journal of the Society for Neuroscience36(26)7014-26(2016 06)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Unique function of Kinesin Kif5A in localization of mitochondria in axons.
Campbell PD, Shen K, Sapio MR, Glenn TD, Talbot WS, Marlow FL
The Journal of neuroscience : the official journal of the Society for Neuroscience34(44)14717-32(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones in zebrafish.
Title: Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones.

Study shows that Kif1b is required for the localization of mbp (myelin basic protein) mRNA to processes of myelinating oligodendrocytes in zebrafish.
Title: Kif1b is essential for mRNA localization in oligodendrocytes and development of myelinated axons.

The following kif1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27807	XM_005162237.4 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40277	XM_009296829.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40278	XM_009296830.2 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40279	XM_009296831.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa53411	NM_194382.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151448	XM_021469758.1 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151449	XM_021469759.1 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa57345	XM_017353527.2 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa40261	XM_009296812.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40262	XM_009296813.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40263	XM_009296814.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40264	XM_009296815.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40265	XM_009296816.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40266	XM_009296817.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40267	XM_009296818.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40268	XM_009296820.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40269	XM_009296821.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40270	XM_009296822.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40271	XM_009296823.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40272	XM_009296824.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40273	XM_009296825.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40274	XM_009296826.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40275	XM_009296827.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40276	XM_009296828.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40280	XM_009296832.3 Latest version!	Danio rerio kinesin family member 1B (kif1b), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa40264	XM_009296815.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40268	XM_009296820.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40280	XM_009296832.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40277	XM_009296829.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40262	XM_009296813.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40266	XM_009296817.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40263	XM_009296814.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40269	XM_009296821.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40272	XM_009296824.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40267	XM_009296818.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40279	XM_009296831.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa27807	XM_005162237.3	Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40261	XM_009296812.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40270	XM_009296822.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40274	XM_009296826.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40275	XM_009296827.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40265	XM_009296816.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40273	XM_009296825.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40276	XM_009296828.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40271	XM_009296823.2	Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa57345	XM_017353527.1	Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif1b ( XM_005162237.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162294.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ODa27807
Clone ID Related Accession (Same CDS sequence)	XM_005162237.4 , XM_005162237.3
Accession Version	XM_005162237.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162237.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGATGGG 
GACTCTGATA GTGGTGATGA CTCTGATAAG CGGTCATGTG AGGAGAGCTG GAGACTCATC 
ACTTCTCTAC GGGAGAAGCT TCCTCCCAGC AAGCTCCAAA CCATTGTCAA AAAGTGCGGT 
TTACCCAGCA GCGGTAAAAG ACGAGAGCCT GTCAAAATGT ACCAGATCCC TCAGCGTCGT 
CGCCTTACAA AAGACTCCAA ATGGGTGACC ATCTCAGATC TGAAGATCCA GGCAGTCAAA 
GAAATTTGCT ATGAAGTGGC ACTGAATGAT TTCCGTCATA GCCGCCAAGA GATAGAGGCT 
CTAGCCATAG TAAAATTGAA GGAGCTTTGT GCCACCTACA ACAAGAAAGA CCCAAACGAA 
CGAGATTCTT GGAGAGCAGT AGCTCGAGAC GTCTGGGACA CTGTAGGAGT CGGGGACGAA 
CGAATCGAAG ATGTCATAAC CAACGGTAGA GGTGTGGCTG ATATGGATGA CCTGAAGGTG 
CACATTGACA AACTAGAGGA CATCCTGCAT GAGGTAAAAA AGCAAAACAA CACGAAAGAT 
GAAGAGATCC GAGCTCTCCG CACTAAGATG TTGAAGATGG AGCAAGTGAT CCCACTGATT 
TCCAACGAAA ATCTGTCCCA GGAGAAGACC TCTCGTGGCG GACCCATGAG GGCCCGCAGT 
TCCAGCGAGG GTAAAGCTCT TGAGGAGAAG CCCAGTGACG AACAGTCAGA TGATCAGATG 
TGCAAGATGG TAGACGACGA TTCTTCGTTT CGACGTGGGC GCATGAGGTG GATGCGACAA 
GAACAAGTGC GCCTCAAGAA CCTCCAGCAA CAGGAAATTT CCAAGCAACT CAGAAGGCAA 
ACTGGACCTC ACAGATTCAT CCCACCTGAA GACCGCAAGC TGCGCTTCCC CTTTAAGAGT 
AACCCTAAAC ATCGCAACTC CTGGACTCCT GGCACCCATA TTATTATCAC CGATGAACAG 
GTGATTGAAT TAAAAGTTCC CAAAGAAGCA GTCCAAGAAG AGAACGATGA CACCGAAGAG 
TCTGTAGAGC CCAGACCACA AGTGGTCCCC ACTATCCAGA CATATCCCCC TCTTCCTAGT 
CCCATGGGTC AACGTAAAAG CAAAGATCTA GAGCAAGGTG TGAGTCATAG ACCTAATCAA 
AGCCAGAAAC TCCACGAACG AGGTCGTAGT AACTCCTTTA ACAGCAGCCA GCGAGCCTCG 
GACTCTACAG AGACCTTCAA TTCTGGAAAC CGGAAGCCCC CTCACCCCCA ACAGGCCTTT 
TACCAGCCAC GCTACAACCA AAACCAGCAT TATTATCCAC CACAACCGCA TCCACAGTTC 
TTTCATTACA ATACTGATGC CGGCTACCAG CAATACCCAC CCAATCAGCA GCACAGTCAC 
CACAACAATT TTAACACGCC ACCCCGTATG CGTAGGCAAT TGTCTGCCCC CAATTTGAAG 
GGCAGTCGAG AAACCGCAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162294.1
CDS	324..3767
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_005162237.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162237.4

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGATGGG 
GACTCTGATA GTGGTGATGA CTCTGATAAG CGGTCATGTG AGGAGAGCTG GAGACTCATC 
ACTTCTCTAC GGGAGAAGCT TCCTCCCAGC AAGCTCCAAA CCATTGTCAA AAAGTGCGGT 
TTACCCAGCA GCGGTAAAAG ACGAGAGCCT GTCAAAATGT ACCAGATCCC TCAGCGTCGT 
CGCCTTACAA AAGACTCCAA ATGGGTGACC ATCTCAGATC TGAAGATCCA GGCAGTCAAA 
GAAATTTGCT ATGAAGTGGC ACTGAATGAT TTCCGTCATA GCCGCCAAGA GATAGAGGCT 
CTAGCCATAG TAAAATTGAA GGAGCTTTGT GCCACCTACA ACAAGAAAGA CCCAAACGAA 
CGAGATTCTT GGAGAGCAGT AGCTCGAGAC GTCTGGGACA CTGTAGGAGT CGGGGACGAA 
CGAATCGAAG ATGTCATAAC CAACGGTAGA GGTGTGGCTG ATATGGATGA CCTGAAGGTG 
CACATTGACA AACTAGAGGA CATCCTGCAT GAGGTAAAAA AGCAAAACAA CACGAAAGAT 
GAAGAGATCC GAGCTCTCCG CACTAAGATG TTGAAGATGG AGCAAGTGAT CCCACTGATT 
TCCAACGAAA ATCTGTCCCA GGAGAAGACC TCTCGTGGCG GACCCATGAG GGCCCGCAGT 
TCCAGCGAGG GTAAAGCTCT TGAGGAGAAG CCCAGTGACG AACAGTCAGA TGATCAGATG 
TGCAAGATGG TAGACGACGA TTCTTCGTTT CGACGTGGGC GCATGAGGTG GATGCGACAA 
GAACAAGTGC GCCTCAAGAA CCTCCAGCAA CAGGAAATTT CCAAGCAACT CAGAAGGCAA 
ACTGGACCTC ACAGATTCAT CCCACCTGAA GACCGCAAGC TGCGCTTCCC CTTTAAGAGT 
AACCCTAAAC ATCGCAACTC CTGGACTCCT GGCACCCATA TTATTATCAC CGATGAACAG 
GTGATTGAAT TAAAAGTTCC CAAAGAAGCA GTCCAAGAAG AGAACGATGA CACCGAAGAG 
TCTGTAGAGC CCAGACCACA AGTGGTCCCC ACTATCCAGA CATATCCCCC TCTTCCTAGT 
CCCATGGGTC AACGTAAAAG CAAAGATCTA GAGCAAGGTG TGAGTCATAG ACCTAATCAA 
AGCCAGAAAC TCCACGAACG AGGTCGTAGT AACTCCTTTA ACAGCAGCCA GCGAGCCTCG 
GACTCTACAG AGACCTTCAA TTCTGGAAAC CGGAAGCCCC CTCACCCCCA ACAGGCCTTT 
TACCAGCCAC GCTACAACCA AAACCAGCAT TATTATCCAC CACAACCGCA TCCACAGTTC 
TTTCATTACA ATACTGATGC CGGCTACCAG CAATACCCAC CCAATCAGCA GCACAGTCAC 
CACAACAATT TTAACACGCC ACCCCGTATG CGTAGGCAAT TGTCTGCCCC CAATTTGAAG 
GGCAGTCGAG AAACCGCAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_005162237.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162294.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.

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CloneID	ODa27807
Clone ID Related Accession (Same CDS sequence)	XM_005162237.4 , XM_005162237.3
Accession Version	XM_005162237.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGATGGG 
GACTCTGATA GTGGTGATGA CTCTGATAAG CGGTCATGTG AGGAGAGCTG GAGACTCATC 
ACTTCTCTAC GGGAGAAGCT TCCTCCCAGC AAGCTCCAAA CCATTGTCAA AAAGTGCGGT 
TTACCCAGCA GCGGTAAAAG ACGAGAGCCT GTCAAAATGT ACCAGATCCC TCAGCGTCGT 
CGCCTTACAA AAGACTCCAA ATGGGTGACC ATCTCAGATC TGAAGATCCA GGCAGTCAAA 
GAAATTTGCT ATGAAGTGGC ACTGAATGAT TTCCGTCATA GCCGCCAAGA GATAGAGGCT 
CTAGCCATAG TAAAATTGAA GGAGCTTTGT GCCACCTACA ACAAGAAAGA CCCAAACGAA 
CGAGATTCTT GGAGAGCAGT AGCTCGAGAC GTCTGGGACA CTGTAGGAGT CGGGGACGAA 
CGAATCGAAG ATGTCATAAC CAACGGTAGA GGTGTGGCTG ATATGGATGA CCTGAAGGTG 
CACATTGACA AACTAGAGGA CATCCTGCAT GAGGTAAAAA AGCAAAACAA CACGAAAGAT 
GAAGAGATCC GAGCTCTCCG CACTAAGATG TTGAAGATGG AGCAAGTGAT CCCACTGATT 
TCCAACGAAA ATCTGTCCCA GGAGAAGACC TCTCGTGGCG GACCCATGAG GGCCCGCAGT 
TCCAGCGAGG GTAAAGCTCT TGAGGAGAAG CCCAGTGACG AACAGTCAGA TGATCAGATG 
TGCAAGATGG TAGACGACGA TTCTTCGTTT CGACGTGGGC GCATGAGGTG GATGCGACAA 
GAACAAGTGC GCCTCAAGAA CCTCCAGCAA CAGGAAATTT CCAAGCAACT CAGAAGGCAA 
ACTGGACCTC ACAGATTCAT CCCACCTGAA GACCGCAAGC TGCGCTTCCC CTTTAAGAGT 
AACCCTAAAC ATCGCAACTC CTGGACTCCT GGCACCCATA TTATTATCAC CGATGAACAG 
GTGATTGAAT TAAAAGTTCC CAAAGAAGCA GTCCAAGAAG AGAACGATGA CACCGAAGAG 
TCTGTAGAGC CCAGACCACA AGTGGTCCCC ACTATCCAGA CATATCCCCC TCTTCCTAGT 
CCCATGGGTC AACGTAAAAG CAAAGATCTA GAGCAAGGTG TGAGTCATAG ACCTAATCAA 
AGCCAGAAAC TCCACGAACG AGGTCGTAGT AACTCCTTTA ACAGCAGCCA GCGAGCCTCG 
GACTCTACAG AGACCTTCAA TTCTGGAAAC CGGAAGCCCC CTCACCCCCA ACAGGCCTTT 
TACCAGCCAC GCTACAACCA AAACCAGCAT TATTATCCAC CACAACCGCA TCCACAGTTC 
TTTCATTACA ATACTGATGC CGGCTACCAG CAATACCCAC CCAATCAGCA GCACAGTCAC 
CACAACAATT TTAACACGCC ACCCCGTATG CGTAGGCAAT TGTCTGCCCC CAATTTGAAG 
GGCAGTCGAG AAACCGCAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162294.1
CDS	319..3762
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_005162237.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162237.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGATGGG 
GACTCTGATA GTGGTGATGA CTCTGATAAG CGGTCATGTG AGGAGAGCTG GAGACTCATC 
ACTTCTCTAC GGGAGAAGCT TCCTCCCAGC AAGCTCCAAA CCATTGTCAA AAAGTGCGGT 
TTACCCAGCA GCGGTAAAAG ACGAGAGCCT GTCAAAATGT ACCAGATCCC TCAGCGTCGT 
CGCCTTACAA AAGACTCCAA ATGGGTGACC ATCTCAGATC TGAAGATCCA GGCAGTCAAA 
GAAATTTGCT ATGAAGTGGC ACTGAATGAT TTCCGTCATA GCCGCCAAGA GATAGAGGCT 
CTAGCCATAG TAAAATTGAA GGAGCTTTGT GCCACCTACA ACAAGAAAGA CCCAAACGAA 
CGAGATTCTT GGAGAGCAGT AGCTCGAGAC GTCTGGGACA CTGTAGGAGT CGGGGACGAA 
CGAATCGAAG ATGTCATAAC CAACGGTAGA GGTGTGGCTG ATATGGATGA CCTGAAGGTG 
CACATTGACA AACTAGAGGA CATCCTGCAT GAGGTAAAAA AGCAAAACAA CACGAAAGAT 
GAAGAGATCC GAGCTCTCCG CACTAAGATG TTGAAGATGG AGCAAGTGAT CCCACTGATT 
TCCAACGAAA ATCTGTCCCA GGAGAAGACC TCTCGTGGCG GACCCATGAG GGCCCGCAGT 
TCCAGCGAGG GTAAAGCTCT TGAGGAGAAG CCCAGTGACG AACAGTCAGA TGATCAGATG 
TGCAAGATGG TAGACGACGA TTCTTCGTTT CGACGTGGGC GCATGAGGTG GATGCGACAA 
GAACAAGTGC GCCTCAAGAA CCTCCAGCAA CAGGAAATTT CCAAGCAACT CAGAAGGCAA 
ACTGGACCTC ACAGATTCAT CCCACCTGAA GACCGCAAGC TGCGCTTCCC CTTTAAGAGT 
AACCCTAAAC ATCGCAACTC CTGGACTCCT GGCACCCATA TTATTATCAC CGATGAACAG 
GTGATTGAAT TAAAAGTTCC CAAAGAAGCA GTCCAAGAAG AGAACGATGA CACCGAAGAG 
TCTGTAGAGC CCAGACCACA AGTGGTCCCC ACTATCCAGA CATATCCCCC TCTTCCTAGT 
CCCATGGGTC AACGTAAAAG CAAAGATCTA GAGCAAGGTG TGAGTCATAG ACCTAATCAA 
AGCCAGAAAC TCCACGAACG AGGTCGTAGT AACTCCTTTA ACAGCAGCCA GCGAGCCTCG 
GACTCTACAG AGACCTTCAA TTCTGGAAAC CGGAAGCCCC CTCACCCCCA ACAGGCCTTT 
TACCAGCCAC GCTACAACCA AAACCAGCAT TATTATCCAC CACAACCGCA TCCACAGTTC 
TTTCATTACA ATACTGATGC CGGCTACCAG CAATACCCAC CCAATCAGCA GCACAGTCAC 
CACAACAATT TTAACACGCC ACCCCGTATG CGTAGGCAAT TGTCTGCCCC CAATTTGAAG 
GGCAGTCGAG AAACCGCAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296829.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295104.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa40277
Clone ID Related Accession (Same CDS sequence)	XM_009296829.3 , XM_009296829.2
Accession Version	XM_009296829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG ATGGGGACTC TGATAGTGGT GATGACTCTG ATAAGCGGTC ATGTGAGGAG 
AGCTGGAGAC TCATCACTTC TCTACGGGAG AAGCTTCCTC CCAGCAAGCT CCAAACCATT 
GTCAAAAAGT GCGGTTTACC CAGCAGCGGT AAAAGACGAG AGCCTGTCAA AATGTACCAG 
ATCCCTCAGC GTCGTCGCCT TACAAAAGAC TCCAAATGGG TGACCATCTC AGATCTGAAG 
ATCCAGGCAG TCAAAGAAAT TTGCTATGAA GTGGCACTGA ATGATTTCCG TCATAGCCGC 
CAAGAGATAG AGGCTCTAGC CATAGTAAAA TTGAAGGAGC TTTGTGCCAC CTACAACAAG 
AAAGACCCAA ACGAACGAGA TTCTTGGAGA GCAGTAGCTC GAGACGTCTG GGACACTGTA 
GGAGTCGGGG ACGAACGAAT CGAAGATGTC ATAACCAACG GTAGAGGTGT GGCTGATATG 
GATGACCTGA AGGTGCACAT TGACAAACTA GAGGACATCC TGCATGAGGT AAAAAAGCAA 
AACAACACGA AAGATGAAGA GATCCGAGCT CTCCGCACTA AGATGTTGAA GATGGAGCAA 
GTGATCCCAC TGATTTCCAA CGAAAATCTG TCCCAGGAGA AGACCTCTCG TGGCGGACCC 
ATGAGGGCCC GCAGTTCCAG CGAGGGTAAA GCTCTTGAGG AGAAGCCCAG TGACGAACAG 
TCAGATGATC AGATGTGCAA GATGGTAGAC GACGATTCTT CGTTTCGACG TGGGCGCATG 
AGGTGGATGC GACAAGAACA AGTGCGCCTC AAGAACCTCC AGCAACAGGA AATTTCCAAG 
CAACTCAGAA GGCAAACTGG ACCTCACAGA TTCATCCCAC CTGAAGACCG CAAGCTGCGC 
TTCCCCTTTA AGAGTAACCC TAAACATCGC AACTCCTGGA CTCCTGGCAC CCATATTATT 
ATCACCGATG AACAGGTGAT TGAATTAAAA GTTCCCAAAG AAGCAGTCCA AGAAGAGAAC 
GATGACACCG AAGAGTCTGT AGAGCCCAGA CCACAAGTGG TCCCCACTAT CCAGACATAT 
CCCCCTCTTC CTAGTCCCAT GGGTCAACGT AAAAGCAAAG ATCTAGAGCA AGGTGTGAGT 
CATAGACCTA ATCAAAGCCA GAAACTCCAC GAACGAGGTC GTAGTAACTC CTTTAACAGC 
AGCCAGCGAG CCTCGGACTC TACAGAGACC TTCAATTCTG GAAACCGGAA GCCCCCTCAC 
CCCCAACAGG CCTTTTACCA GCCACGCTAC AACCAAAACC AGCATTATTA TCCACCACAA 
CCGCATCCAC AGTTCTTTCA TTACAATACT GATGCCGGCT ACCAGCAATA CCCACCCAAT 
CAGCAGCACA GTCACCACAA CAATTTTAAC ACGCCACCCC GTATGCGTAG GCAATTGTCT 
GCCCCCAATT TGAAGGGCAG TCGAGAAACC GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295104.1
CDS	325..4023
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_009296829.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296829.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG ATGGGGACTC TGATAGTGGT GATGACTCTG ATAAGCGGTC ATGTGAGGAG 
AGCTGGAGAC TCATCACTTC TCTACGGGAG AAGCTTCCTC CCAGCAAGCT CCAAACCATT 
GTCAAAAAGT GCGGTTTACC CAGCAGCGGT AAAAGACGAG AGCCTGTCAA AATGTACCAG 
ATCCCTCAGC GTCGTCGCCT TACAAAAGAC TCCAAATGGG TGACCATCTC AGATCTGAAG 
ATCCAGGCAG TCAAAGAAAT TTGCTATGAA GTGGCACTGA ATGATTTCCG TCATAGCCGC 
CAAGAGATAG AGGCTCTAGC CATAGTAAAA TTGAAGGAGC TTTGTGCCAC CTACAACAAG 
AAAGACCCAA ACGAACGAGA TTCTTGGAGA GCAGTAGCTC GAGACGTCTG GGACACTGTA 
GGAGTCGGGG ACGAACGAAT CGAAGATGTC ATAACCAACG GTAGAGGTGT GGCTGATATG 
GATGACCTGA AGGTGCACAT TGACAAACTA GAGGACATCC TGCATGAGGT AAAAAAGCAA 
AACAACACGA AAGATGAAGA GATCCGAGCT CTCCGCACTA AGATGTTGAA GATGGAGCAA 
GTGATCCCAC TGATTTCCAA CGAAAATCTG TCCCAGGAGA AGACCTCTCG TGGCGGACCC 
ATGAGGGCCC GCAGTTCCAG CGAGGGTAAA GCTCTTGAGG AGAAGCCCAG TGACGAACAG 
TCAGATGATC AGATGTGCAA GATGGTAGAC GACGATTCTT CGTTTCGACG TGGGCGCATG 
AGGTGGATGC GACAAGAACA AGTGCGCCTC AAGAACCTCC AGCAACAGGA AATTTCCAAG 
CAACTCAGAA GGCAAACTGG ACCTCACAGA TTCATCCCAC CTGAAGACCG CAAGCTGCGC 
TTCCCCTTTA AGAGTAACCC TAAACATCGC AACTCCTGGA CTCCTGGCAC CCATATTATT 
ATCACCGATG AACAGGTGAT TGAATTAAAA GTTCCCAAAG AAGCAGTCCA AGAAGAGAAC 
GATGACACCG AAGAGTCTGT AGAGCCCAGA CCACAAGTGG TCCCCACTAT CCAGACATAT 
CCCCCTCTTC CTAGTCCCAT GGGTCAACGT AAAAGCAAAG ATCTAGAGCA AGGTGTGAGT 
CATAGACCTA ATCAAAGCCA GAAACTCCAC GAACGAGGTC GTAGTAACTC CTTTAACAGC 
AGCCAGCGAG CCTCGGACTC TACAGAGACC TTCAATTCTG GAAACCGGAA GCCCCCTCAC 
CCCCAACAGG CCTTTTACCA GCCACGCTAC AACCAAAACC AGCATTATTA TCCACCACAA 
CCGCATCCAC AGTTCTTTCA TTACAATACT GATGCCGGCT ACCAGCAATA CCCACCCAAT 
CAGCAGCACA GTCACCACAA CAATTTTAAC ACGCCACCCC GTATGCGTAG GCAATTGTCT 
GCCCCCAATT TGAAGGGCAG TCGAGAAACC GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296829.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295104.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.

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CloneID	ODa40277
Clone ID Related Accession (Same CDS sequence)	XM_009296829.3 , XM_009296829.2
Accession Version	XM_009296829.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG ATGGGGACTC TGATAGTGGT GATGACTCTG ATAAGCGGTC ATGTGAGGAG 
AGCTGGAGAC TCATCACTTC TCTACGGGAG AAGCTTCCTC CCAGCAAGCT CCAAACCATT 
GTCAAAAAGT GCGGTTTACC CAGCAGCGGT AAAAGACGAG AGCCTGTCAA AATGTACCAG 
ATCCCTCAGC GTCGTCGCCT TACAAAAGAC TCCAAATGGG TGACCATCTC AGATCTGAAG 
ATCCAGGCAG TCAAAGAAAT TTGCTATGAA GTGGCACTGA ATGATTTCCG TCATAGCCGC 
CAAGAGATAG AGGCTCTAGC CATAGTAAAA TTGAAGGAGC TTTGTGCCAC CTACAACAAG 
AAAGACCCAA ACGAACGAGA TTCTTGGAGA GCAGTAGCTC GAGACGTCTG GGACACTGTA 
GGAGTCGGGG ACGAACGAAT CGAAGATGTC ATAACCAACG GTAGAGGTGT GGCTGATATG 
GATGACCTGA AGGTGCACAT TGACAAACTA GAGGACATCC TGCATGAGGT AAAAAAGCAA 
AACAACACGA AAGATGAAGA GATCCGAGCT CTCCGCACTA AGATGTTGAA GATGGAGCAA 
GTGATCCCAC TGATTTCCAA CGAAAATCTG TCCCAGGAGA AGACCTCTCG TGGCGGACCC 
ATGAGGGCCC GCAGTTCCAG CGAGGGTAAA GCTCTTGAGG AGAAGCCCAG TGACGAACAG 
TCAGATGATC AGATGTGCAA GATGGTAGAC GACGATTCTT CGTTTCGACG TGGGCGCATG 
AGGTGGATGC GACAAGAACA AGTGCGCCTC AAGAACCTCC AGCAACAGGA AATTTCCAAG 
CAACTCAGAA GGCAAACTGG ACCTCACAGA TTCATCCCAC CTGAAGACCG CAAGCTGCGC 
TTCCCCTTTA AGAGTAACCC TAAACATCGC AACTCCTGGA CTCCTGGCAC CCATATTATT 
ATCACCGATG AACAGGTGAT TGAATTAAAA GTTCCCAAAG AAGCAGTCCA AGAAGAGAAC 
GATGACACCG AAGAGTCTGT AGAGCCCAGA CCACAAGTGG TCCCCACTAT CCAGACATAT 
CCCCCTCTTC CTAGTCCCAT GGGTCAACGT AAAAGCAAAG ATCTAGAGCA AGGTGTGAGT 
CATAGACCTA ATCAAAGCCA GAAACTCCAC GAACGAGGTC GTAGTAACTC CTTTAACAGC 
AGCCAGCGAG CCTCGGACTC TACAGAGACC TTCAATTCTG GAAACCGGAA GCCCCCTCAC 
CCCCAACAGG CCTTTTACCA GCCACGCTAC AACCAAAACC AGCATTATTA TCCACCACAA 
CCGCATCCAC AGTTCTTTCA TTACAATACT GATGCCGGCT ACCAGCAATA CCCACCCAAT 
CAGCAGCACA GTCACCACAA CAATTTTAAC ACGCCACCCC GTATGCGTAG GCAATTGTCT 
GCCCCCAATT TGAAGGGCAG TCGAGAAACC GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295104.1
CDS	319..4017
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_009296829.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296829.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG ATGGGGACTC TGATAGTGGT GATGACTCTG ATAAGCGGTC ATGTGAGGAG 
AGCTGGAGAC TCATCACTTC TCTACGGGAG AAGCTTCCTC CCAGCAAGCT CCAAACCATT 
GTCAAAAAGT GCGGTTTACC CAGCAGCGGT AAAAGACGAG AGCCTGTCAA AATGTACCAG 
ATCCCTCAGC GTCGTCGCCT TACAAAAGAC TCCAAATGGG TGACCATCTC AGATCTGAAG 
ATCCAGGCAG TCAAAGAAAT TTGCTATGAA GTGGCACTGA ATGATTTCCG TCATAGCCGC 
CAAGAGATAG AGGCTCTAGC CATAGTAAAA TTGAAGGAGC TTTGTGCCAC CTACAACAAG 
AAAGACCCAA ACGAACGAGA TTCTTGGAGA GCAGTAGCTC GAGACGTCTG GGACACTGTA 
GGAGTCGGGG ACGAACGAAT CGAAGATGTC ATAACCAACG GTAGAGGTGT GGCTGATATG 
GATGACCTGA AGGTGCACAT TGACAAACTA GAGGACATCC TGCATGAGGT AAAAAAGCAA 
AACAACACGA AAGATGAAGA GATCCGAGCT CTCCGCACTA AGATGTTGAA GATGGAGCAA 
GTGATCCCAC TGATTTCCAA CGAAAATCTG TCCCAGGAGA AGACCTCTCG TGGCGGACCC 
ATGAGGGCCC GCAGTTCCAG CGAGGGTAAA GCTCTTGAGG AGAAGCCCAG TGACGAACAG 
TCAGATGATC AGATGTGCAA GATGGTAGAC GACGATTCTT CGTTTCGACG TGGGCGCATG 
AGGTGGATGC GACAAGAACA AGTGCGCCTC AAGAACCTCC AGCAACAGGA AATTTCCAAG 
CAACTCAGAA GGCAAACTGG ACCTCACAGA TTCATCCCAC CTGAAGACCG CAAGCTGCGC 
TTCCCCTTTA AGAGTAACCC TAAACATCGC AACTCCTGGA CTCCTGGCAC CCATATTATT 
ATCACCGATG AACAGGTGAT TGAATTAAAA GTTCCCAAAG AAGCAGTCCA AGAAGAGAAC 
GATGACACCG AAGAGTCTGT AGAGCCCAGA CCACAAGTGG TCCCCACTAT CCAGACATAT 
CCCCCTCTTC CTAGTCCCAT GGGTCAACGT AAAAGCAAAG ATCTAGAGCA AGGTGTGAGT 
CATAGACCTA ATCAAAGCCA GAAACTCCAC GAACGAGGTC GTAGTAACTC CTTTAACAGC 
AGCCAGCGAG CCTCGGACTC TACAGAGACC TTCAATTCTG GAAACCGGAA GCCCCCTCAC 
CCCCAACAGG CCTTTTACCA GCCACGCTAC AACCAAAACC AGCATTATTA TCCACCACAA 
CCGCATCCAC AGTTCTTTCA TTACAATACT GATGCCGGCT ACCAGCAATA CCCACCCAAT 
CAGCAGCACA GTCACCACAA CAATTTTAAC ACGCCACCCC GTATGCGTAG GCAATTGTCT 
GCCCCCAATT TGAAGGGCAG TCGAGAAACC GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296830.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295105.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.

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CloneID	ODa40278
Clone ID Related Accession (Same CDS sequence)	XM_009296830.2
Accession Version	XM_009296830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGATGGGG ACTCTGATAG TGGTGATGAC TCTGATAAGC GGTCATGTGA GGAGAGCTGG 
AGACTCATCA CTTCTCTACG GGAGAAGCTT CCTCCCAGCA AGCTCCAAAC CATTGTCAAA 
AAGTGCGGTT TACCCAGCAG CGGTAAAAGA CGAGAGCCTG TCAAAATGTA CCAGATCCCT 
CAGCGTCGTC GCCTTACAAA AGACTCCAAA TGGGTGACCA TCTCAGATCT GAAGATCCAG 
GCAGTCAAAG AAATTTGCTA TGAAGTGGCA CTGAATGATT TCCGTCATAG CCGCCAAGAG 
ATAGAGGCTC TAGCCATAGT AAAATTGAAG GAGCTTTGTG CCACCTACAA CAAGAAAGAC 
CCAAACGAAC GAGATTCTTG GAGAGCAGTA GCTCGAGACG TCTGGGACAC TGTAGGAGTC 
GGGGACGAAC GAATCGAAGA TGTCATAACC AACGGTAGAG GTGTGGCTGA TATGGATGAC 
CTGAAGGTGC ACATTGACAA ACTAGAGGAC ATCCTGCATG AGGTAAAAAA GCAAAACAAC 
ACGAAAGATG AAGAGATCCG AGCTCTCCGC ACTAAGATGT TGAAGATGGA GCAAGTGATC 
CCACTGATTT CCAACGAAAA TCTGTCCCAG GAGAAGACCT CTCGTGGCGG ACCCATGAGG 
GCCCGCAGTT CCAGCGAGGG TAAAGCTCTT GAGGAGAAGC CCAGTGACGA ACAGTCAGAT 
GATCAGATGT GCAAGATGGT AGACGACGAT TCTTCGTTTC GACGTGGGCG CATGAGGTGG 
ATGCGACAAG AACAAGTGCG CCTCAAGAAC CTCCAGCAAC AGGAAATTTC CAAGCAACTC 
AGAAGGCAAA CTGGACCTCA CAGATTCATC CCACCTGAAG ACCGCAAGCT GCGCTTCCCC 
TTTAAGAGTA ACCCTAAACA TCGCAACTCC TGGACTCCTG GCACCCATAT TATTATCACC 
GATGAACAGG TGATTGAATT AAAAGTTCCC AAAGAAGCAG TCCAAGAAGA GAACGATGAC 
ACCGAAGAGT CTGTAGAGCC CAGACCACAA GTGGTCCCCA CTATCCAGAC ATATCCCCCT 
CTTCCTAGTC CCATGGGTCA ACGTAAAAGC AAAGATCTAG AGCAAGGTGT GAGTCATAGA 
CCTAATCAAA GCCAGAAACT CCACGAACGA GGTCGTAGTA ACTCCTTTAA CAGCAGCCAG 
CGAGCCTCGG ACTCTACAGA GACCTTCAAT TCTGGAAACC GGAAGCCCCC TCACCCCCAA 
CAGGCCTTTT ACCAGCCACG CTACAACCAA AACCAGCATT ATTATCCACC ACAACCGCAT 
CCACAGTTCT TTCATTACAA TACTGATGCC GGCTACCAGC AATACCCACC CAATCAGCAG 
CACAGTCACC ACAACAATTT TAACACGCCA CCCCGTATGC GTAGGCAATT GTCTGCCCCC 
AATTTGAAGG GCAGTCGAGA AACCGCAGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295105.1
CDS	319..3891
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_009296830.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296830.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGATGGGG ACTCTGATAG TGGTGATGAC TCTGATAAGC GGTCATGTGA GGAGAGCTGG 
AGACTCATCA CTTCTCTACG GGAGAAGCTT CCTCCCAGCA AGCTCCAAAC CATTGTCAAA 
AAGTGCGGTT TACCCAGCAG CGGTAAAAGA CGAGAGCCTG TCAAAATGTA CCAGATCCCT 
CAGCGTCGTC GCCTTACAAA AGACTCCAAA TGGGTGACCA TCTCAGATCT GAAGATCCAG 
GCAGTCAAAG AAATTTGCTA TGAAGTGGCA CTGAATGATT TCCGTCATAG CCGCCAAGAG 
ATAGAGGCTC TAGCCATAGT AAAATTGAAG GAGCTTTGTG CCACCTACAA CAAGAAAGAC 
CCAAACGAAC GAGATTCTTG GAGAGCAGTA GCTCGAGACG TCTGGGACAC TGTAGGAGTC 
GGGGACGAAC GAATCGAAGA TGTCATAACC AACGGTAGAG GTGTGGCTGA TATGGATGAC 
CTGAAGGTGC ACATTGACAA ACTAGAGGAC ATCCTGCATG AGGTAAAAAA GCAAAACAAC 
ACGAAAGATG AAGAGATCCG AGCTCTCCGC ACTAAGATGT TGAAGATGGA GCAAGTGATC 
CCACTGATTT CCAACGAAAA TCTGTCCCAG GAGAAGACCT CTCGTGGCGG ACCCATGAGG 
GCCCGCAGTT CCAGCGAGGG TAAAGCTCTT GAGGAGAAGC CCAGTGACGA ACAGTCAGAT 
GATCAGATGT GCAAGATGGT AGACGACGAT TCTTCGTTTC GACGTGGGCG CATGAGGTGG 
ATGCGACAAG AACAAGTGCG CCTCAAGAAC CTCCAGCAAC AGGAAATTTC CAAGCAACTC 
AGAAGGCAAA CTGGACCTCA CAGATTCATC CCACCTGAAG ACCGCAAGCT GCGCTTCCCC 
TTTAAGAGTA ACCCTAAACA TCGCAACTCC TGGACTCCTG GCACCCATAT TATTATCACC 
GATGAACAGG TGATTGAATT AAAAGTTCCC AAAGAAGCAG TCCAAGAAGA GAACGATGAC 
ACCGAAGAGT CTGTAGAGCC CAGACCACAA GTGGTCCCCA CTATCCAGAC ATATCCCCCT 
CTTCCTAGTC CCATGGGTCA ACGTAAAAGC AAAGATCTAG AGCAAGGTGT GAGTCATAGA 
CCTAATCAAA GCCAGAAACT CCACGAACGA GGTCGTAGTA ACTCCTTTAA CAGCAGCCAG 
CGAGCCTCGG ACTCTACAGA GACCTTCAAT TCTGGAAACC GGAAGCCCCC TCACCCCCAA 
CAGGCCTTTT ACCAGCCACG CTACAACCAA AACCAGCATT ATTATCCACC ACAACCGCAT 
CCACAGTTCT TTCATTACAA TACTGATGCC GGCTACCAGC AATACCCACC CAATCAGCAG 
CACAGTCACC ACAACAATTT TAACACGCCA CCCCGTATGC GTAGGCAATT GTCTGCCCCC 
AATTTGAAGG GCAGTCGAGA AACCGCAGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296831.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295106.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa40279
Clone ID Related Accession (Same CDS sequence)	XM_009296831.3 , XM_009296831.2
Accession Version	XM_009296831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TCACCCATGG GCTGTCTGAC AGCCTCTCCA TCTTCAGGCT CTCTCTGCAG CCAGGCAGGC 
CTACAGTCCG TCTCCAGCAT CCAGGAGCGC ATCATGTCCA CGCCAGGCGG AGAAGAGGCC 
ATCGAGAGGC TCAAGGAATC AGAAAAGATC ATAGCCGAGC TGAATGAGAC CTGGGAAGAG 
AAGCTGAGGA AGACCGAGGC CATCCGTATG GAGAGAGAGG CGCTGCTGGC AGAGATGGGA 
GTTGCCATAC GGGAGGACGG GGGAACCCTG GGCGTCTTCT CTCCTAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGATG GGGACTCTGA TAGTGGTGAT 
GACTCTGATA AGCGGTCATG TGAGGAGAGC TGGAGACTCA TCACTTCTCT ACGGGAGAAG 
CTTCCTCCCA GCAAGCTCCA AACCATTGTC AAAAAGTGCG GTTTACCCAG CAGCGGTAAA 
AGACGAGAGC CTGTCAAAAT GTACCAGATC CCTCAGCGTC GTCGCCTTAC AAAAGACTCC 
AAATGGGTGA CCATCTCAGA TCTGAAGATC CAGGCAGTCA AAGAAATTTG CTATGAAGTG 
GCACTGAATG ATTTCCGTCA TAGCCGCCAA GAGATAGAGG CTCTAGCCAT AGTAAAATTG 
AAGGAGCTTT GTGCCACCTA CAACAAGAAA GACCCAAACG AACGAGATTC TTGGAGAGCA 
GTAGCTCGAG ACGTCTGGGA CACTGTAGGA GTCGGGGACG AACGAATCGA AGATGTCATA 
ACCAACGGTA GAGGTGTGGC TGATATGGAT GACCTGAAGG TGCACATTGA CAAACTAGAG 
GACATCCTGC ATGAGGTAAA AAAGCAAAAC AACACGAAAG ATGAAGAGAT CCGAGCTCTC 
CGCACTAAGA TGTTGAAGAT GGAGCAAGTG ATCCCACTGA TTTCCAACGA AAATCTGTCC 
CAGGAGAAGA CCTCTCGTGG CGGACCCATG AGGGCCCGCA GTTCCAGCGA GGGTAAAGCT 
CTTGAGGAGA AGCCCAGTGA CGAACAGTCA GATGATCAGA TGTGCAAGAT GGTAGACGAC 
GATTCTTCGT TTCGACGTGG GCGCATGAGG TGGATGCGAC AAGAACAAGT GCGCCTCAAG 
AACCTCCAGC AACAGGAAAT TTCCAAGCAA CTCAGAAGGC AAACTGGACC TCACAGATTC 
ATCCCACCTG AAGACCGCAA GCTGCGCTTC CCCTTTAAGA GTAACCCTAA ACATCGCAAC 
TCCTGGACTC CTGGCACCCA TATTATTATC ACCGATGAAC AGGTGATTGA ATTAAAAGTT 
CCCAAAGAAG CAGTCCAAGA AGAGAACGAT GACACCGAAG AGTCTGTAGA GCCCAGACCA 
CAAGTGGTCC CCACTATCCA GACATATCCC CCTCTTCCTA GTCCCATGGG TCAACGTAAA 
AGCAAAGATC TAGAGCAAGG TGTGAGTCAT AGACCTAATC AAAGCCAGAA ACTCCACGAA 
CGAGGTCGTA GTAACTCCTT TAACAGCAGC CAGCGAGCCT CGGACTCTAC AGAGACCTTC 
AATTCTGGAA ACCGGAAGCC CCCTCACCCC CAACAGGCCT TTTACCAGCC ACGCTACAAC 
CAAAACCAGC ATTATTATCC ACCACAACCG CATCCACAGT TCTTTCATTA CAATACTGAT 
GCCGGCTACC AGCAATACCC ACCCAATCAG CAGCACAGTC ACCACAACAA TTTTAACACG 
CCACCCCGTA TGCGTAGGCA ATTGTCTGCC CCCAATTTGA AGGGCAGTCG AGAAACCGCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295106.1
CDS	325..3810
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_009296831.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296831.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TCACCCATGG GCTGTCTGAC AGCCTCTCCA TCTTCAGGCT CTCTCTGCAG CCAGGCAGGC 
CTACAGTCCG TCTCCAGCAT CCAGGAGCGC ATCATGTCCA CGCCAGGCGG AGAAGAGGCC 
ATCGAGAGGC TCAAGGAATC AGAAAAGATC ATAGCCGAGC TGAATGAGAC CTGGGAAGAG 
AAGCTGAGGA AGACCGAGGC CATCCGTATG GAGAGAGAGG CGCTGCTGGC AGAGATGGGA 
GTTGCCATAC GGGAGGACGG GGGAACCCTG GGCGTCTTCT CTCCTAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGATG GGGACTCTGA TAGTGGTGAT 
GACTCTGATA AGCGGTCATG TGAGGAGAGC TGGAGACTCA TCACTTCTCT ACGGGAGAAG 
CTTCCTCCCA GCAAGCTCCA AACCATTGTC AAAAAGTGCG GTTTACCCAG CAGCGGTAAA 
AGACGAGAGC CTGTCAAAAT GTACCAGATC CCTCAGCGTC GTCGCCTTAC AAAAGACTCC 
AAATGGGTGA CCATCTCAGA TCTGAAGATC CAGGCAGTCA AAGAAATTTG CTATGAAGTG 
GCACTGAATG ATTTCCGTCA TAGCCGCCAA GAGATAGAGG CTCTAGCCAT AGTAAAATTG 
AAGGAGCTTT GTGCCACCTA CAACAAGAAA GACCCAAACG AACGAGATTC TTGGAGAGCA 
GTAGCTCGAG ACGTCTGGGA CACTGTAGGA GTCGGGGACG AACGAATCGA AGATGTCATA 
ACCAACGGTA GAGGTGTGGC TGATATGGAT GACCTGAAGG TGCACATTGA CAAACTAGAG 
GACATCCTGC ATGAGGTAAA AAAGCAAAAC AACACGAAAG ATGAAGAGAT CCGAGCTCTC 
CGCACTAAGA TGTTGAAGAT GGAGCAAGTG ATCCCACTGA TTTCCAACGA AAATCTGTCC 
CAGGAGAAGA CCTCTCGTGG CGGACCCATG AGGGCCCGCA GTTCCAGCGA GGGTAAAGCT 
CTTGAGGAGA AGCCCAGTGA CGAACAGTCA GATGATCAGA TGTGCAAGAT GGTAGACGAC 
GATTCTTCGT TTCGACGTGG GCGCATGAGG TGGATGCGAC AAGAACAAGT GCGCCTCAAG 
AACCTCCAGC AACAGGAAAT TTCCAAGCAA CTCAGAAGGC AAACTGGACC TCACAGATTC 
ATCCCACCTG AAGACCGCAA GCTGCGCTTC CCCTTTAAGA GTAACCCTAA ACATCGCAAC 
TCCTGGACTC CTGGCACCCA TATTATTATC ACCGATGAAC AGGTGATTGA ATTAAAAGTT 
CCCAAAGAAG CAGTCCAAGA AGAGAACGAT GACACCGAAG AGTCTGTAGA GCCCAGACCA 
CAAGTGGTCC CCACTATCCA GACATATCCC CCTCTTCCTA GTCCCATGGG TCAACGTAAA 
AGCAAAGATC TAGAGCAAGG TGTGAGTCAT AGACCTAATC AAAGCCAGAA ACTCCACGAA 
CGAGGTCGTA GTAACTCCTT TAACAGCAGC CAGCGAGCCT CGGACTCTAC AGAGACCTTC 
AATTCTGGAA ACCGGAAGCC CCCTCACCCC CAACAGGCCT TTTACCAGCC ACGCTACAAC 
CAAAACCAGC ATTATTATCC ACCACAACCG CATCCACAGT TCTTTCATTA CAATACTGAT 
GCCGGCTACC AGCAATACCC ACCCAATCAG CAGCACAGTC ACCACAACAA TTTTAACACG 
CCACCCCGTA TGCGTAGGCA ATTGTCTGCC CCCAATTTGA AGGGCAGTCG AGAAACCGCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296831.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295106.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.

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CloneID	ODa40279
Clone ID Related Accession (Same CDS sequence)	XM_009296831.3 , XM_009296831.2
Accession Version	XM_009296831.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TCACCCATGG GCTGTCTGAC AGCCTCTCCA TCTTCAGGCT CTCTCTGCAG CCAGGCAGGC 
CTACAGTCCG TCTCCAGCAT CCAGGAGCGC ATCATGTCCA CGCCAGGCGG AGAAGAGGCC 
ATCGAGAGGC TCAAGGAATC AGAAAAGATC ATAGCCGAGC TGAATGAGAC CTGGGAAGAG 
AAGCTGAGGA AGACCGAGGC CATCCGTATG GAGAGAGAGG CGCTGCTGGC AGAGATGGGA 
GTTGCCATAC GGGAGGACGG GGGAACCCTG GGCGTCTTCT CTCCTAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGATG GGGACTCTGA TAGTGGTGAT 
GACTCTGATA AGCGGTCATG TGAGGAGAGC TGGAGACTCA TCACTTCTCT ACGGGAGAAG 
CTTCCTCCCA GCAAGCTCCA AACCATTGTC AAAAAGTGCG GTTTACCCAG CAGCGGTAAA 
AGACGAGAGC CTGTCAAAAT GTACCAGATC CCTCAGCGTC GTCGCCTTAC AAAAGACTCC 
AAATGGGTGA CCATCTCAGA TCTGAAGATC CAGGCAGTCA AAGAAATTTG CTATGAAGTG 
GCACTGAATG ATTTCCGTCA TAGCCGCCAA GAGATAGAGG CTCTAGCCAT AGTAAAATTG 
AAGGAGCTTT GTGCCACCTA CAACAAGAAA GACCCAAACG AACGAGATTC TTGGAGAGCA 
GTAGCTCGAG ACGTCTGGGA CACTGTAGGA GTCGGGGACG AACGAATCGA AGATGTCATA 
ACCAACGGTA GAGGTGTGGC TGATATGGAT GACCTGAAGG TGCACATTGA CAAACTAGAG 
GACATCCTGC ATGAGGTAAA AAAGCAAAAC AACACGAAAG ATGAAGAGAT CCGAGCTCTC 
CGCACTAAGA TGTTGAAGAT GGAGCAAGTG ATCCCACTGA TTTCCAACGA AAATCTGTCC 
CAGGAGAAGA CCTCTCGTGG CGGACCCATG AGGGCCCGCA GTTCCAGCGA GGGTAAAGCT 
CTTGAGGAGA AGCCCAGTGA CGAACAGTCA GATGATCAGA TGTGCAAGAT GGTAGACGAC 
GATTCTTCGT TTCGACGTGG GCGCATGAGG TGGATGCGAC AAGAACAAGT GCGCCTCAAG 
AACCTCCAGC AACAGGAAAT TTCCAAGCAA CTCAGAAGGC AAACTGGACC TCACAGATTC 
ATCCCACCTG AAGACCGCAA GCTGCGCTTC CCCTTTAAGA GTAACCCTAA ACATCGCAAC 
TCCTGGACTC CTGGCACCCA TATTATTATC ACCGATGAAC AGGTGATTGA ATTAAAAGTT 
CCCAAAGAAG CAGTCCAAGA AGAGAACGAT GACACCGAAG AGTCTGTAGA GCCCAGACCA 
CAAGTGGTCC CCACTATCCA GACATATCCC CCTCTTCCTA GTCCCATGGG TCAACGTAAA 
AGCAAAGATC TAGAGCAAGG TGTGAGTCAT AGACCTAATC AAAGCCAGAA ACTCCACGAA 
CGAGGTCGTA GTAACTCCTT TAACAGCAGC CAGCGAGCCT CGGACTCTAC AGAGACCTTC 
AATTCTGGAA ACCGGAAGCC CCCTCACCCC CAACAGGCCT TTTACCAGCC ACGCTACAAC 
CAAAACCAGC ATTATTATCC ACCACAACCG CATCCACAGT TCTTTCATTA CAATACTGAT 
GCCGGCTACC AGCAATACCC ACCCAATCAG CAGCACAGTC ACCACAACAA TTTTAACACG 
CCACCCCGTA TGCGTAGGCA ATTGTCTGCC CCCAATTTGA AGGGCAGTCG AGAAACCGCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295106.1
CDS	319..3804
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLDGDSDSGDDSDKRSCEESWRLITSLREKLPPSKLQTIVKKCGLPSSGKRREPVKMYQIPQRRRLTKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKLKELCATYNKKDPNERDSWRAVARDVWDTVGVGDERIEDVITNGRGVADMDDLKVHIDKLEDILHEVKKQNNTKDEEIRALRTKMLKMEQVIPLISNENLSQEKTSRGGPMRARSSSEGKALEEKPSDEQSDDQMCKMVDDDSSFRRGRMRWMRQEQVRLKNLQQQEISKQLRRQTGPHRFIPPEDRKLRFPFKSNPKHRNSWTPGTHIIITDEQVIELKVPKEAVQEENDDTEESVEPRPQVVPTIQTYPPLPSPMGQRKSKDLEQGVSHRPNQSQKLHERGRSNSFNSSQRASDSTETFNSGNRKPPHPQQAFYQPRYNQNQHYYPPQPHPQFFHYNTDAGYQQYPPNQQHSHHNNFNTPPRMRRQLSAPNLKGSRETAV

Target ORF information:

RefSeq Version	XM_009296831.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296831.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TCACCCATGG GCTGTCTGAC AGCCTCTCCA TCTTCAGGCT CTCTCTGCAG CCAGGCAGGC 
CTACAGTCCG TCTCCAGCAT CCAGGAGCGC ATCATGTCCA CGCCAGGCGG AGAAGAGGCC 
ATCGAGAGGC TCAAGGAATC AGAAAAGATC ATAGCCGAGC TGAATGAGAC CTGGGAAGAG 
AAGCTGAGGA AGACCGAGGC CATCCGTATG GAGAGAGAGG CGCTGCTGGC AGAGATGGGA 
GTTGCCATAC GGGAGGACGG GGGAACCCTG GGCGTCTTCT CTCCTAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGATG GGGACTCTGA TAGTGGTGAT 
GACTCTGATA AGCGGTCATG TGAGGAGAGC TGGAGACTCA TCACTTCTCT ACGGGAGAAG 
CTTCCTCCCA GCAAGCTCCA AACCATTGTC AAAAAGTGCG GTTTACCCAG CAGCGGTAAA 
AGACGAGAGC CTGTCAAAAT GTACCAGATC CCTCAGCGTC GTCGCCTTAC AAAAGACTCC 
AAATGGGTGA CCATCTCAGA TCTGAAGATC CAGGCAGTCA AAGAAATTTG CTATGAAGTG 
GCACTGAATG ATTTCCGTCA TAGCCGCCAA GAGATAGAGG CTCTAGCCAT AGTAAAATTG 
AAGGAGCTTT GTGCCACCTA CAACAAGAAA GACCCAAACG AACGAGATTC TTGGAGAGCA 
GTAGCTCGAG ACGTCTGGGA CACTGTAGGA GTCGGGGACG AACGAATCGA AGATGTCATA 
ACCAACGGTA GAGGTGTGGC TGATATGGAT GACCTGAAGG TGCACATTGA CAAACTAGAG 
GACATCCTGC ATGAGGTAAA AAAGCAAAAC AACACGAAAG ATGAAGAGAT CCGAGCTCTC 
CGCACTAAGA TGTTGAAGAT GGAGCAAGTG ATCCCACTGA TTTCCAACGA AAATCTGTCC 
CAGGAGAAGA CCTCTCGTGG CGGACCCATG AGGGCCCGCA GTTCCAGCGA GGGTAAAGCT 
CTTGAGGAGA AGCCCAGTGA CGAACAGTCA GATGATCAGA TGTGCAAGAT GGTAGACGAC 
GATTCTTCGT TTCGACGTGG GCGCATGAGG TGGATGCGAC AAGAACAAGT GCGCCTCAAG 
AACCTCCAGC AACAGGAAAT TTCCAAGCAA CTCAGAAGGC AAACTGGACC TCACAGATTC 
ATCCCACCTG AAGACCGCAA GCTGCGCTTC CCCTTTAAGA GTAACCCTAA ACATCGCAAC 
TCCTGGACTC CTGGCACCCA TATTATTATC ACCGATGAAC AGGTGATTGA ATTAAAAGTT 
CCCAAAGAAG CAGTCCAAGA AGAGAACGAT GACACCGAAG AGTCTGTAGA GCCCAGACCA 
CAAGTGGTCC CCACTATCCA GACATATCCC CCTCTTCCTA GTCCCATGGG TCAACGTAAA 
AGCAAAGATC TAGAGCAAGG TGTGAGTCAT AGACCTAATC AAAGCCAGAA ACTCCACGAA 
CGAGGTCGTA GTAACTCCTT TAACAGCAGC CAGCGAGCCT CGGACTCTAC AGAGACCTTC 
AATTCTGGAA ACCGGAAGCC CCCTCACCCC CAACAGGCCT TTTACCAGCC ACGCTACAAC 
CAAAACCAGC ATTATTATCC ACCACAACCG CATCCACAGT TCTTTCATTA CAATACTGAT 
GCCGGCTACC AGCAATACCC ACCCAATCAG CAGCACAGTC ACCACAACAA TTTTAACACG 
CCACCCCGTA TGCGTAGGCA ATTGTCTGCC CCCAATTTGA AGGGCAGTCG AGAAACCGCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( NM_194382.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919363.3 Danio rerio kinesin family member 1B (kif1b), mRNA.

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CloneID	ODa53411
Clone ID Related Accession (Same CDS sequence)	NM_194382.3
Accession Version	NM_194382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571850.7 and CR381616.9. On Feb 5, 2016 this sequence version replaced NM_194382.2. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ756939.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505381, SAMEA3505392 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGTACAC ACTTCACACG 
CAAAAGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919363.3
CDS	90..5402
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRYTLHTQKTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	NM_194382.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194382.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGTACAC ACTTCACACG 
CAAAAGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_021469758.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325433.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.

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CloneID	ODa151448
Clone ID Related Accession (Same CDS sequence)	XM_021469758.1
Accession Version	XM_021469758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAACC TGAAAGATAT ACACAACAAT 
AAGCATAGAT ACTTGCTTGC CTCAGAGAAC CAACGGCCTG GCCATTTCTC CACAGCACCC 
ATGGGCTGTC TGACAGCCTC TCCATCTTCA GGCTCTCTCT GCAGCCAGGC AGGCCTACAG 
TCCGTCTCCA GCATCCAGGA GCGCATCATG TCCACGCCAG GCGGAGAAGA GGCCATCGAG 
AGGCTCAAGG AATCAGAAAA GATCATAGCC GAGCTGAATG AGACCTGGGA AGAGAAGCTG 
AGGAAGACCG AGGCCATCCG TATGGAGAGA GAGGCGCTGC TGGCAGAGAT GGGAGTTGCC 
ATACGGGAGG ACGGGGGAAC CCTGGGCGTC TTCTCTCCTA AAAAGTTTGG TCCTGATAGA 
CCAACGTCAC TGTCCATTGA GGACTTTAGT GTTTATTCTT CCAATCAGCT TTTCGTTGAA 
AGGCCCTGTG AAGTCTATAC TGACTTTAAT GGGGTGTTTT CACCCAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGTGT ATGAGAGCAA ACTGCAAGAG 
CTGCAGAAAC AAGTGGAGAC TCGTTCTTTG GCTGCAGAGA CTCCAGATGA GGAGGAAGAA 
GAAGAGGAGG AGGATGAAGT GCCCTGGACC CAGCATAAAT ATGAGCTGGC TCAGTGGGCT 
TTCAGGAAGT GGAGATACCA CCAGTTCACC TCCCTCAGAG ACCAATTATG GGGCAATGCT 
GTGTACCTCA AAGAAGCCAA TGCCATCAGT GTGGAGCTCA AGAAAAAGGT CCAGTTCCAG 
TTCGTCTTGC TCACAGACAC CCTCTATTCT CCACTGCCCC CGGAGCTGCT GCCTCCAGAG 
CCAGAGAAAG AGCGAGACGC CAGGCCTTTT CCGAGAACAG TGGTGGCTGT GGAGGTTCAG 
GACCTGAAGA ATGGAGCCAC TCACTACTGG TCCCTGGAGA AGCTGAAGCA GAGGCTGGAC 
CAGATGCGAG AGATGTACGA CCGTGCTGGC GAAATGGCCT CGTCCAATCA GGGAGATGAT 
GGGGAGGGTG CGTTGACCGG CAGTGACCCG TTCTATGACC GATTTCATTG GTTTAAGCTG 
GTTGGGAGCT CACCTATTTT TCATGGCTGT GTGAACGAGC GTCTGGCCGA CCGCACACCA 
TCGCCCACCT TCTCCACCAC CGATTCGGAG ATCACTGAGC TGGCCGACGA GCGACAGAGC 
GAGATGGAGG ACTTTATGGA TGACGAGGCT TTCGTGGATG ACACCAGCTC AGATGCTGGA 
ACCGAGGAGG GCTCGGACAT CTTCAGTGAT GGCCAGGACC CCTTCTATGA TCGTTCTCCA 
TGGTTCATCC TGGTGGGCAG AGCCTTCGTG TACCTGAGTA ACCTGCTGTA CCCTGTTCCT 
CTGGTGCATC GAGTCGCTGT AGTGACAGAG AAAGGAGATG TGCGAGGATT TCTGCGTGTG 
GGGGTCCAGG CCATCGCCGC GGATGAAGAG GCACCTGATT ATGGGTCTGG TGTGCGTCAG 
TCAGGAACTG CTAAGATTTC CTTTGATAAT GAGTATTTTA AAAAGAATGA CTTCACCTCA 
GTGGCCATGA CCCGCTCTGG TCTCTCTCTG GAGGAGCTGC GCATTGTAGA AGGTCAAGGT 
CAGAGTTCAG AGGTCATTAC GCCTTCAGAG GAGATGAACA GAATCAATGA AATGGACCTA 
AAGCTGGGGA ACGTGGACAC AAAACTGGGA GACGGTTTGG CCGAGCATCT GGAAGTGGGC 
AGCATTTTCA CTTTCCGCGT CACCGTTCTG CAGGCAAGCG GCATCCCGGC CGAATACGCA 
GACATCTTCT GCCAGTTCAA TTTCCTCCAC CGTCACGATG AGGCGTTCAG CACTGAGCCT 
CTGAAGAACA CTGGCAGAGG AGCCCCGCTG GGCTTTTACC ACGTACAGAA TATCTCAGTG 
GAGGTGACAG AGTCCTTCAT TGAGTACATA AAGACCAAGC CAATTGTATT TGAAGTGTTT 
GGCCATTACC AGCAACACCC ACTGCACATT CATGGACAGG ATGTGGTCAG CCCTTCTCAG 
CCTTCCAGAA AGTACTATCC TCCACCAATG CCTCTGTCTA AACCAGATCA CGCTCGTAAG 
ATTGAGCTTA TCCGTTACCT GATGAGCGGG TATGTTCATG GTCAGGTGTT GGACACATTG 
TCAGAGCATG CCAACGCTTT GGCCTCCACC GCCGTGGCCA GTCTGGAGGA AGGGGCATAT 
GAGCTGTGCC ACTTCCCCTT CCTCCCCATA CCCAACTGCC CCGTCTTCAG AGTGCCCCGA 
CACGACGTGC CAGCCACCAA ACTGAACACC ATCACCAAGT CCAACCTGGG TCAGTGTGTG 
AGCAAGTACG ACTTGCTGGC CTGGTTTGAG ATCAGCGAAC TGGAGCCCAC TGGAGAGTAT 
ATTCCAGCAG TAGTGGATCA TACAGGAGGT CTTCCCTGTC ATGGCACATA CCTGCTGCAC 
CAGGGCATCC AGCGCAGAAT AACAGTGACT CTGATCCATG AGAAGGGAAG TGAGCTGCAC 
TGGAAGGACG TCCGAGAGCT GGTGGTCGGT CGAATCAGGA ATAAGCCAGA AGTGGATGAT 
TCAGCCGCAG ACGCCGTCTT GTCTCTCAAC ATCATCTCAG CTAAAAACCT CAAATCCTCC 
CACAACTCCA GCAGCCTTTG TATTGATAGC GATATTTCTA GGACGTTCTA CCGGTTTGAG 
GCCGTGTGGG ACAGTTCTCT GCACAACTCT CTCCTACTGA ACCGCGTCAC TCCATACGGA 
GAGAAGATCT ACATGACCCT GTCTGCCTAT CTGGAGCTCG ATCACTGCAT CCAGCCTGCT 
ATCATCACCA AGGACATCTG TATGGTCTTC TACTCCAGAG ATGCCAAGAT CTCGCCTCCT 
CGCTCGCTGA GAAACTTGTT TGGCAGCGGC TACTCCAAAA CCCCGGACTG TAATAAAGTG 
ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG ATAGCAGACA CCGGAAGCCC AGGTATGCAG 
CGGCGCAGAC GGAAGGTTCT GGACACGTCA GTGGCATATG TGCGTGGAGA GGAGAATCTG 
GCCGGCTGGA GACCCCGAGG AGACAGTCTG ATCCTGGAGC ACCAGTGGGA GCTGGACAAA 
ATGGAGCAGC TGCATGAGGT GGAAAAGACG CGTCACTTGC TTCTGCTGCG TGAGAAGCTG 
GGAGAAACAC CTCCACCTAA ATCTCTGAGC GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG 
ACCCTCAGCA CCTCCACCAG CATCTCCTCC CAGATCTCCT CCACAACTTT CGAGAGTGCC 
ATCACCCCCA GCGAAAGCAG CGGCTATGAC TCCACGGACA TCGAGAGCTT GGTGGATCGA 
GAGAAGGAGC TGGCCACTAA GTGCCTCCGT CTCCTGACAC ACACTTTCAA CAGTGAATAC 
AACCAAATGT GCAACAGCAT TAGTGACTGC AAGTTTTCAG ATATCTCTCC TATGGGTCGG 
GATCCATCAG TCAGTAGTTT CAGCAGTGCC ACCCTCACAC CCTCCTCCAC CTGCCCCTCG 
CTCTCAGACT CCAGGACCCC TGAAGCAAAC TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC 
TATGAGAACT TCCCCATGGT GCCCATTCTG GAGACGTCCT ACCTGGCCCG CGCCGGCAAA 
CACGAGTTCC TCAACCTTGT GCCTAACATA GAGGAAATGA GGCCTGGGTC TGTGGTGTCC 
AAGAAGGGCA TCTTGAATTT CATGGAGCCC CGCTCGAACA CCTGGGTGAA GCACTTTGTG 
GTGGTGCGTC GGCCGTATGT GTTCATCTAC AACAATGACA AGGACCCTGT GGAGAGAGGA 
GTGCTGAACT TATCCACTGC ACAGGTGGAG TACAGTGAAG ACCAGCAGGC CATGCTCAAG 
ACGCCTCATA CATTTGCCAT GTGCACCAAA CACAGAGGAA TCCTGCTTCA GGCCAACAAT 
GACAAAGACA TGAACGACTG GCTGTATGCC TTTAACCCAC TGCTAGCAGG AACAATAAGG 
TCGAAGCTTG CAAGGAGACG GTCTGGTGTG ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325433.1
CDS	320..6061
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDNLKDIHNNKHRYLLASENQRPGHFSTAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_021469758.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469758.1

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAACC TGAAAGATAT ACACAACAAT 
AAGCATAGAT ACTTGCTTGC CTCAGAGAAC CAACGGCCTG GCCATTTCTC CACAGCACCC 
ATGGGCTGTC TGACAGCCTC TCCATCTTCA GGCTCTCTCT GCAGCCAGGC AGGCCTACAG 
TCCGTCTCCA GCATCCAGGA GCGCATCATG TCCACGCCAG GCGGAGAAGA GGCCATCGAG 
AGGCTCAAGG AATCAGAAAA GATCATAGCC GAGCTGAATG AGACCTGGGA AGAGAAGCTG 
AGGAAGACCG AGGCCATCCG TATGGAGAGA GAGGCGCTGC TGGCAGAGAT GGGAGTTGCC 
ATACGGGAGG ACGGGGGAAC CCTGGGCGTC TTCTCTCCTA AAAAGTTTGG TCCTGATAGA 
CCAACGTCAC TGTCCATTGA GGACTTTAGT GTTTATTCTT CCAATCAGCT TTTCGTTGAA 
AGGCCCTGTG AAGTCTATAC TGACTTTAAT GGGGTGTTTT CACCCAAAAA GACGCCTCAC 
CTGGTGAACC TGAACGAGGA TCCTTTGATG TCAGAATGTC TGCTCTATTA TATCAAAGAT 
GGAATAACAA GGGTGGGCCA GGCAGATGCT GAAAGAAGAC AGGATATCGT TCTAAGTGGA 
GCTCACATTA AAGAGGAGCA CTGTATTTTC CGCAGTGAGA GGAATGCCAA TGGACATGTG 
ATTGTCACGT TGGAGCCATG TGAGGGATCA GAAACATATG TAAATGGTAA ACGTGTGAAT 
TCTGCGGTCC AGCTGCGCTC AGGTAACCGC ATTATCATGG GAAAGAACCA CGTGTTCCGG 
TTTAACCACC CTGAGCAGGC TCGCGCTGAG CGTGAGAAGA CCCCGTCAGC AGAGACACCT 
GTAGAACCTG TTGACTGGAC TTTTGCCCAA AGAGAACTGC TGGAGAAACA GGGTATCGAC 
ATGAAGCAAG AGATGGAGAA GAGGCTTACA GAAATGGAGA TCCTGTACAA AAAAGAGAAG 
GAGGAAGCGG ACCAGCTACT GGAGCAACAG AGACTGGTGT ATGAGAGCAA ACTGCAAGAG 
CTGCAGAAAC AAGTGGAGAC TCGTTCTTTG GCTGCAGAGA CTCCAGATGA GGAGGAAGAA 
GAAGAGGAGG AGGATGAAGT GCCCTGGACC CAGCATAAAT ATGAGCTGGC TCAGTGGGCT 
TTCAGGAAGT GGAGATACCA CCAGTTCACC TCCCTCAGAG ACCAATTATG GGGCAATGCT 
GTGTACCTCA AAGAAGCCAA TGCCATCAGT GTGGAGCTCA AGAAAAAGGT CCAGTTCCAG 
TTCGTCTTGC TCACAGACAC CCTCTATTCT CCACTGCCCC CGGAGCTGCT GCCTCCAGAG 
CCAGAGAAAG AGCGAGACGC CAGGCCTTTT CCGAGAACAG TGGTGGCTGT GGAGGTTCAG 
GACCTGAAGA ATGGAGCCAC TCACTACTGG TCCCTGGAGA AGCTGAAGCA GAGGCTGGAC 
CAGATGCGAG AGATGTACGA CCGTGCTGGC GAAATGGCCT CGTCCAATCA GGGAGATGAT 
GGGGAGGGTG CGTTGACCGG CAGTGACCCG TTCTATGACC GATTTCATTG GTTTAAGCTG 
GTTGGGAGCT CACCTATTTT TCATGGCTGT GTGAACGAGC GTCTGGCCGA CCGCACACCA 
TCGCCCACCT TCTCCACCAC CGATTCGGAG ATCACTGAGC TGGCCGACGA GCGACAGAGC 
GAGATGGAGG ACTTTATGGA TGACGAGGCT TTCGTGGATG ACACCAGCTC AGATGCTGGA 
ACCGAGGAGG GCTCGGACAT CTTCAGTGAT GGCCAGGACC CCTTCTATGA TCGTTCTCCA 
TGGTTCATCC TGGTGGGCAG AGCCTTCGTG TACCTGAGTA ACCTGCTGTA CCCTGTTCCT 
CTGGTGCATC GAGTCGCTGT AGTGACAGAG AAAGGAGATG TGCGAGGATT TCTGCGTGTG 
GGGGTCCAGG CCATCGCCGC GGATGAAGAG GCACCTGATT ATGGGTCTGG TGTGCGTCAG 
TCAGGAACTG CTAAGATTTC CTTTGATAAT GAGTATTTTA AAAAGAATGA CTTCACCTCA 
GTGGCCATGA CCCGCTCTGG TCTCTCTCTG GAGGAGCTGC GCATTGTAGA AGGTCAAGGT 
CAGAGTTCAG AGGTCATTAC GCCTTCAGAG GAGATGAACA GAATCAATGA AATGGACCTA 
AAGCTGGGGA ACGTGGACAC AAAACTGGGA GACGGTTTGG CCGAGCATCT GGAAGTGGGC 
AGCATTTTCA CTTTCCGCGT CACCGTTCTG CAGGCAAGCG GCATCCCGGC CGAATACGCA 
GACATCTTCT GCCAGTTCAA TTTCCTCCAC CGTCACGATG AGGCGTTCAG CACTGAGCCT 
CTGAAGAACA CTGGCAGAGG AGCCCCGCTG GGCTTTTACC ACGTACAGAA TATCTCAGTG 
GAGGTGACAG AGTCCTTCAT TGAGTACATA AAGACCAAGC CAATTGTATT TGAAGTGTTT 
GGCCATTACC AGCAACACCC ACTGCACATT CATGGACAGG ATGTGGTCAG CCCTTCTCAG 
CCTTCCAGAA AGTACTATCC TCCACCAATG CCTCTGTCTA AACCAGATCA CGCTCGTAAG 
ATTGAGCTTA TCCGTTACCT GATGAGCGGG TATGTTCATG GTCAGGTGTT GGACACATTG 
TCAGAGCATG CCAACGCTTT GGCCTCCACC GCCGTGGCCA GTCTGGAGGA AGGGGCATAT 
GAGCTGTGCC ACTTCCCCTT CCTCCCCATA CCCAACTGCC CCGTCTTCAG AGTGCCCCGA 
CACGACGTGC CAGCCACCAA ACTGAACACC ATCACCAAGT CCAACCTGGG TCAGTGTGTG 
AGCAAGTACG ACTTGCTGGC CTGGTTTGAG ATCAGCGAAC TGGAGCCCAC TGGAGAGTAT 
ATTCCAGCAG TAGTGGATCA TACAGGAGGT CTTCCCTGTC ATGGCACATA CCTGCTGCAC 
CAGGGCATCC AGCGCAGAAT AACAGTGACT CTGATCCATG AGAAGGGAAG TGAGCTGCAC 
TGGAAGGACG TCCGAGAGCT GGTGGTCGGT CGAATCAGGA ATAAGCCAGA AGTGGATGAT 
TCAGCCGCAG ACGCCGTCTT GTCTCTCAAC ATCATCTCAG CTAAAAACCT CAAATCCTCC 
CACAACTCCA GCAGCCTTTG TATTGATAGC GATATTTCTA GGACGTTCTA CCGGTTTGAG 
GCCGTGTGGG ACAGTTCTCT GCACAACTCT CTCCTACTGA ACCGCGTCAC TCCATACGGA 
GAGAAGATCT ACATGACCCT GTCTGCCTAT CTGGAGCTCG ATCACTGCAT CCAGCCTGCT 
ATCATCACCA AGGACATCTG TATGGTCTTC TACTCCAGAG ATGCCAAGAT CTCGCCTCCT 
CGCTCGCTGA GAAACTTGTT TGGCAGCGGC TACTCCAAAA CCCCGGACTG TAATAAAGTG 
ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG ATAGCAGACA CCGGAAGCCC AGGTATGCAG 
CGGCGCAGAC GGAAGGTTCT GGACACGTCA GTGGCATATG TGCGTGGAGA GGAGAATCTG 
GCCGGCTGGA GACCCCGAGG AGACAGTCTG ATCCTGGAGC ACCAGTGGGA GCTGGACAAA 
ATGGAGCAGC TGCATGAGGT GGAAAAGACG CGTCACTTGC TTCTGCTGCG TGAGAAGCTG 
GGAGAAACAC CTCCACCTAA ATCTCTGAGC GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG 
ACCCTCAGCA CCTCCACCAG CATCTCCTCC CAGATCTCCT CCACAACTTT CGAGAGTGCC 
ATCACCCCCA GCGAAAGCAG CGGCTATGAC TCCACGGACA TCGAGAGCTT GGTGGATCGA 
GAGAAGGAGC TGGCCACTAA GTGCCTCCGT CTCCTGACAC ACACTTTCAA CAGTGAATAC 
AACCAAATGT GCAACAGCAT TAGTGACTGC AAGTTTTCAG ATATCTCTCC TATGGGTCGG 
GATCCATCAG TCAGTAGTTT CAGCAGTGCC ACCCTCACAC CCTCCTCCAC CTGCCCCTCG 
CTCTCAGACT CCAGGACCCC TGAAGCAAAC TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC 
TATGAGAACT TCCCCATGGT GCCCATTCTG GAGACGTCCT ACCTGGCCCG CGCCGGCAAA 
CACGAGTTCC TCAACCTTGT GCCTAACATA GAGGAAATGA GGCCTGGGTC TGTGGTGTCC 
AAGAAGGGCA TCTTGAATTT CATGGAGCCC CGCTCGAACA CCTGGGTGAA GCACTTTGTG 
GTGGTGCGTC GGCCGTATGT GTTCATCTAC AACAATGACA AGGACCCTGT GGAGAGAGGA 
GTGCTGAACT TATCCACTGC ACAGGTGGAG TACAGTGAAG ACCAGCAGGC CATGCTCAAG 
ACGCCTCATA CATTTGCCAT GTGCACCAAA CACAGAGGAA TCCTGCTTCA GGCCAACAAT 
GACAAAGACA TGAACGACTG GCTGTATGCC TTTAACCCAC TGCTAGCAGG AACAATAAGG 
TCGAAGCTTG CAAGGAGACG GTCTGGTGTG ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_021469759.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325434.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa151449
Clone ID Related Accession (Same CDS sequence)	XM_021469759.1
Accession Version	XM_021469759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAACC TGAAAGATAT ACACAACAAT 
AAGCATAGAT ACTTGCTTGC CTCAGAGAAC CAACGGCCTG GCCATTTCTC CACAGCACCC 
ATGGGCTGTC TGACAGCCTC TCCATCTTCA GGCTCTCTCT GCAGCCAGGC AGGCCTACAG 
TCCGTCTCCA GCATCCAGGA GCGCATCATG TCCACGCCAG GCGGAGAAGA GGCCATCGAG 
AGGCTCAAGG AATCAGAAAA GATCATAGCC GAGCTGAATG AGACCTGGGA AGAGAAGCTG 
AGGAAGACCG AGGCCATCCG TATGGAGAGA GAGGCGCTGC TGGCAGAGAT GGGAGTTGCC 
ATACGGGAGG ACGGGGGAAC CCTGGGCGTC TTCTCTCCTA AAAAGACGCC TCACCTGGTG 
AACCTGAACG AGGATCCTTT GATGTCAGAA TGTCTGCTCT ATTATATCAA AGATGGAATA 
ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA AGACAGGATA TCGTTCTAAG TGGAGCTCAC 
ATTAAAGAGG AGCACTGTAT TTTCCGCAGT GAGAGGAATG CCAATGGACA TGTGATTGTC 
ACGTTGGAGC CATGTGAGGG ATCAGAAACA TATGTAAATG GTAAACGTGT GAATTCTGCG 
GTCCAGCTGC GCTCAGGTAA CCGCATTATC ATGGGAAAGA ACCACGTGTT CCGGTTTAAC 
CACCCTGAGC AGGCTCGCGC TGAGCGTGAG AAGACCCCGT CAGCAGAGAC ACCTGTAGAA 
CCTGTTGACT GGACTTTTGC CCAAAGAGAA CTGCTGGAGA AACAGGGTAT CGACATGAAG 
CAAGAGATGG AGAAGAGGCT TACAGAAATG GAGATCCTGT ACAAAAAAGA GAAGGAGGAA 
GCGGACCAGC TACTGGAGCA ACAGAGACTG GTGTATGAGA GCAAACTGCA AGAGCTGCAG 
AAACAAGTGG AGACTCGTTC TTTGGCTGCA GAGACTCCAG ATGAGGAGGA AGAAGAAGAG 
GAGGAGGATG AAGTGCCCTG GACCCAGCAT AAATATGAGC TGGCTCAGTG GGCTTTCAGG 
AAGTGGAGAT ACCACCAGTT CACCTCCCTC AGAGACCAAT TATGGGGCAA TGCTGTGTAC 
CTCAAAGAAG CCAATGCCAT CAGTGTGGAG CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC 
TTGCTCACAG ACACCCTCTA TTCTCCACTG CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG 
AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA ACAGTGGTGG CTGTGGAGGT TCAGGACCTG 
AAGAATGGAG CCACTCACTA CTGGTCCCTG GAGAAGCTGA AGCAGAGGCT GGACCAGATG 
CGAGAGATGT ACGACCGTGC TGGCGAAATG GCCTCGTCCA ATCAGGGAGA TGATGGGGAG 
GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT GACCGATTTC ATTGGTTTAA GCTGGTTGGG 
AGCTCACCTA TTTTTCATGG CTGTGTGAAC GAGCGTCTGG CCGACCGCAC ACCATCGCCC 
ACCTTCTCCA CCACCGATTC GGAGATCACT GAGCTGGCCG ACGAGCGACA GAGCGAGATG 
GAGGACTTTA TGGATGACGA GGCTTTCGTG GATGACACCA GCTCAGATGC TGGAACCGAG 
GAGGGCTCGG ACATCTTCAG TGATGGCCAG GACCCCTTCT ATGATCGTTC TCCATGGTTC 
ATCCTGGTGG GCAGAGCCTT CGTGTACCTG AGTAACCTGC TGTACCCTGT TCCTCTGGTG 
CATCGAGTCG CTGTAGTGAC AGAGAAAGGA GATGTGCGAG GATTTCTGCG TGTGGGGGTC 
CAGGCCATCG CCGCGGATGA AGAGGCACCT GATTATGGGT CTGGTGTGCG TCAGTCAGGA 
ACTGCTAAGA TTTCCTTTGA TAATGAGTAT TTTAAAAAGA ATGACTTCAC CTCAGTGGCC 
ATGACCCGCT CTGGTCTCTC TCTGGAGGAG CTGCGCATTG TAGAAGGTCA AGGTCAGAGT 
TCAGAGGTCA TTACGCCTTC AGAGGAGATG AACAGAATCA ATGAAATGGA CCTAAAGCTG 
GGGAACGTGG ACACAAAACT GGGAGACGGT TTGGCCGAGC ATCTGGAAGT GGGCAGCATT 
TTCACTTTCC GCGTCACCGT TCTGCAGGCA AGCGGCATCC CGGCCGAATA CGCAGACATC 
TTCTGCCAGT TCAATTTCCT CCACCGTCAC GATGAGGCGT TCAGCACTGA GCCTCTGAAG 
AACACTGGCA GAGGAGCCCC GCTGGGCTTT TACCACGTAC AGAATATCTC AGTGGAGGTG 
ACAGAGTCCT TCATTGAGTA CATAAAGACC AAGCCAATTG TATTTGAAGT GTTTGGCCAT 
TACCAGCAAC ACCCACTGCA CATTCATGGA CAGGATGTGG TCAGCCCTTC TCAGCCTTCC 
AGAAAGTACT ATCCTCCACC AATGCCTCTG TCTAAACCAG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325434.1
CDS	320..5707
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDNLKDIHNNKHRYLLASENQRPGHFSTAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_021469759.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469759.1

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAACC TGAAAGATAT ACACAACAAT 
AAGCATAGAT ACTTGCTTGC CTCAGAGAAC CAACGGCCTG GCCATTTCTC CACAGCACCC 
ATGGGCTGTC TGACAGCCTC TCCATCTTCA GGCTCTCTCT GCAGCCAGGC AGGCCTACAG 
TCCGTCTCCA GCATCCAGGA GCGCATCATG TCCACGCCAG GCGGAGAAGA GGCCATCGAG 
AGGCTCAAGG AATCAGAAAA GATCATAGCC GAGCTGAATG AGACCTGGGA AGAGAAGCTG 
AGGAAGACCG AGGCCATCCG TATGGAGAGA GAGGCGCTGC TGGCAGAGAT GGGAGTTGCC 
ATACGGGAGG ACGGGGGAAC CCTGGGCGTC TTCTCTCCTA AAAAGACGCC TCACCTGGTG 
AACCTGAACG AGGATCCTTT GATGTCAGAA TGTCTGCTCT ATTATATCAA AGATGGAATA 
ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA AGACAGGATA TCGTTCTAAG TGGAGCTCAC 
ATTAAAGAGG AGCACTGTAT TTTCCGCAGT GAGAGGAATG CCAATGGACA TGTGATTGTC 
ACGTTGGAGC CATGTGAGGG ATCAGAAACA TATGTAAATG GTAAACGTGT GAATTCTGCG 
GTCCAGCTGC GCTCAGGTAA CCGCATTATC ATGGGAAAGA ACCACGTGTT CCGGTTTAAC 
CACCCTGAGC AGGCTCGCGC TGAGCGTGAG AAGACCCCGT CAGCAGAGAC ACCTGTAGAA 
CCTGTTGACT GGACTTTTGC CCAAAGAGAA CTGCTGGAGA AACAGGGTAT CGACATGAAG 
CAAGAGATGG AGAAGAGGCT TACAGAAATG GAGATCCTGT ACAAAAAAGA GAAGGAGGAA 
GCGGACCAGC TACTGGAGCA ACAGAGACTG GTGTATGAGA GCAAACTGCA AGAGCTGCAG 
AAACAAGTGG AGACTCGTTC TTTGGCTGCA GAGACTCCAG ATGAGGAGGA AGAAGAAGAG 
GAGGAGGATG AAGTGCCCTG GACCCAGCAT AAATATGAGC TGGCTCAGTG GGCTTTCAGG 
AAGTGGAGAT ACCACCAGTT CACCTCCCTC AGAGACCAAT TATGGGGCAA TGCTGTGTAC 
CTCAAAGAAG CCAATGCCAT CAGTGTGGAG CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC 
TTGCTCACAG ACACCCTCTA TTCTCCACTG CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG 
AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA ACAGTGGTGG CTGTGGAGGT TCAGGACCTG 
AAGAATGGAG CCACTCACTA CTGGTCCCTG GAGAAGCTGA AGCAGAGGCT GGACCAGATG 
CGAGAGATGT ACGACCGTGC TGGCGAAATG GCCTCGTCCA ATCAGGGAGA TGATGGGGAG 
GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT GACCGATTTC ATTGGTTTAA GCTGGTTGGG 
AGCTCACCTA TTTTTCATGG CTGTGTGAAC GAGCGTCTGG CCGACCGCAC ACCATCGCCC 
ACCTTCTCCA CCACCGATTC GGAGATCACT GAGCTGGCCG ACGAGCGACA GAGCGAGATG 
GAGGACTTTA TGGATGACGA GGCTTTCGTG GATGACACCA GCTCAGATGC TGGAACCGAG 
GAGGGCTCGG ACATCTTCAG TGATGGCCAG GACCCCTTCT ATGATCGTTC TCCATGGTTC 
ATCCTGGTGG GCAGAGCCTT CGTGTACCTG AGTAACCTGC TGTACCCTGT TCCTCTGGTG 
CATCGAGTCG CTGTAGTGAC AGAGAAAGGA GATGTGCGAG GATTTCTGCG TGTGGGGGTC 
CAGGCCATCG CCGCGGATGA AGAGGCACCT GATTATGGGT CTGGTGTGCG TCAGTCAGGA 
ACTGCTAAGA TTTCCTTTGA TAATGAGTAT TTTAAAAAGA ATGACTTCAC CTCAGTGGCC 
ATGACCCGCT CTGGTCTCTC TCTGGAGGAG CTGCGCATTG TAGAAGGTCA AGGTCAGAGT 
TCAGAGGTCA TTACGCCTTC AGAGGAGATG AACAGAATCA ATGAAATGGA CCTAAAGCTG 
GGGAACGTGG ACACAAAACT GGGAGACGGT TTGGCCGAGC ATCTGGAAGT GGGCAGCATT 
TTCACTTTCC GCGTCACCGT TCTGCAGGCA AGCGGCATCC CGGCCGAATA CGCAGACATC 
TTCTGCCAGT TCAATTTCCT CCACCGTCAC GATGAGGCGT TCAGCACTGA GCCTCTGAAG 
AACACTGGCA GAGGAGCCCC GCTGGGCTTT TACCACGTAC AGAATATCTC AGTGGAGGTG 
ACAGAGTCCT TCATTGAGTA CATAAAGACC AAGCCAATTG TATTTGAAGT GTTTGGCCAT 
TACCAGCAAC ACCCACTGCA CATTCATGGA CAGGATGTGG TCAGCCCTTC TCAGCCTTCC 
AGAAAGTACT ATCCTCCACC AATGCCTCTG TCTAAACCAG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_017353527.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209016.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.

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CloneID	ODa57345
Clone ID Related Accession (Same CDS sequence)	XM_017353527.1 , XM_017353527.2
Accession Version	XM_017353527.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209016.1
CDS	319..5715
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_017353527.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353527.1

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_017353527.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209016.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.

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CloneID	ODa57345
Clone ID Related Accession (Same CDS sequence)	XM_017353527.1 , XM_017353527.2
Accession Version	XM_017353527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209016.1
CDS	320..5716
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_017353527.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353527.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296812.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295087.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.

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CloneID	ODa40261
Clone ID Related Accession (Same CDS sequence)	XM_009296812.2 , XM_009296812.3
Accession Version	XM_009296812.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGCCT TTGTATTGAT 
AGCGATATTT CTAGGACGTT CTACCGGTTT GAGGCCGTGT GGGACAGTTC TCTGCACAAC 
TCTCTCCTAC TGAACCGCGT CACTCCATAC GGAGAGAAGA TCTACATGAC CCTGTCTGCC 
TATCTGGAGC TCGATCACTG CATCCAGCCT GCTATCATCA CCAAGGACAT CTGTATGGTC 
TTCTACTCCA GAGATGCCAA GATCTCGCCT CCTCGCTCGC TGAGAAACTT GTTTGGCAGC 
GGCTACTCCA AAACCCCGGA CTGTAATAAA GTGACTGGAG TCTATGAGCT GAGCTTGTGC 
AAGATAGCAG ACACCGGAAG CCCAGGTATG CAGCGGCGCA GACGGAAGGT TCTGGACACG 
TCAGTGGCAT ATGTGCGTGG AGAGGAGAAT CTGGCCGGCT GGAGACCCCG AGGAGACAGT 
CTGATCCTGG AGCACCAGTG GGAGCTGGAC AAAATGGAGC AGCTGCATGA GGTGGAAAAG 
ACGCGTCACT TGCTTCTGCT GCGTGAGAAG CTGGGAGAAA CACCTCCACC TAAATCTCTG 
AGCGAGTCTC TGTCCCCCAG CCTGAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCCCAGATCT CCTCCACAAC TTTCGAGAGT GCCATCACCC CCAGCGAAAG CAGCGGCTAT 
GACTCCACGG ACATCGAGAG CTTGGTGGAT CGAGAGAAGG AGCTGGCCAC TAAGTGCCTC 
CGTCTCCTGA CACACACTTT CAACAGTGAA TACAACCAAA TGTGCAACAG CATTAGTGAC 
TGCAAGTTTT CAGATATCTC TCCTATGGGT CGGGATCCAT CAGTCAGTAG TTTCAGCAGT 
GCCACCCTCA CACCCTCCTC CACCTGCCCC TCGCTCTCAG ACTCCAGGAC CCCTGAAGCA 
AACTCTCGTG CAACCAGCCC CTCAGGCTCA GACTATGAGA ACTTCCCCAT GGTGCCCATT 
CTGGAGACGT CCTACCTGGC CCGCGCCGGC AAACACGAGT TCCTCAACCT TGTGCCTAAC 
ATAGAGGAAA TGAGGCCTGG GTCTGTGGTG TCCAAGAAGG GCATCTTGAA TTTCATGGAG 
CCCCGCTCGA ACACCTGGGT GAAGCACTTT GTGGTGGTGC GTCGGCCGTA TGTGTTCATC 
TACAACAATG ACAAGGACCC TGTGGAGAGA GGAGTGCTGA ACTTATCCAC TGCACAGGTG 
GAGTACAGTG AAGACCAGCA GGCCATGCTC AAGACGCCTC ATACATTTGC CATGTGCACC 
AAACACAGAG GAATCCTGCT TCAGGCCAAC AATGACAAAG ACATGAACGA CTGGCTGTAT 
GCCTTTAACC CACTGCTAGC AGGAACAATA AGGTCGAAGC TTGCAAGGAG ACGGTCTGGT 
GTGATGAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295087.1
CDS	319..6093
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296812.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296812.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGCCT TTGTATTGAT 
AGCGATATTT CTAGGACGTT CTACCGGTTT GAGGCCGTGT GGGACAGTTC TCTGCACAAC 
TCTCTCCTAC TGAACCGCGT CACTCCATAC GGAGAGAAGA TCTACATGAC CCTGTCTGCC 
TATCTGGAGC TCGATCACTG CATCCAGCCT GCTATCATCA CCAAGGACAT CTGTATGGTC 
TTCTACTCCA GAGATGCCAA GATCTCGCCT CCTCGCTCGC TGAGAAACTT GTTTGGCAGC 
GGCTACTCCA AAACCCCGGA CTGTAATAAA GTGACTGGAG TCTATGAGCT GAGCTTGTGC 
AAGATAGCAG ACACCGGAAG CCCAGGTATG CAGCGGCGCA GACGGAAGGT TCTGGACACG 
TCAGTGGCAT ATGTGCGTGG AGAGGAGAAT CTGGCCGGCT GGAGACCCCG AGGAGACAGT 
CTGATCCTGG AGCACCAGTG GGAGCTGGAC AAAATGGAGC AGCTGCATGA GGTGGAAAAG 
ACGCGTCACT TGCTTCTGCT GCGTGAGAAG CTGGGAGAAA CACCTCCACC TAAATCTCTG 
AGCGAGTCTC TGTCCCCCAG CCTGAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCCCAGATCT CCTCCACAAC TTTCGAGAGT GCCATCACCC CCAGCGAAAG CAGCGGCTAT 
GACTCCACGG ACATCGAGAG CTTGGTGGAT CGAGAGAAGG AGCTGGCCAC TAAGTGCCTC 
CGTCTCCTGA CACACACTTT CAACAGTGAA TACAACCAAA TGTGCAACAG CATTAGTGAC 
TGCAAGTTTT CAGATATCTC TCCTATGGGT CGGGATCCAT CAGTCAGTAG TTTCAGCAGT 
GCCACCCTCA CACCCTCCTC CACCTGCCCC TCGCTCTCAG ACTCCAGGAC CCCTGAAGCA 
AACTCTCGTG CAACCAGCCC CTCAGGCTCA GACTATGAGA ACTTCCCCAT GGTGCCCATT 
CTGGAGACGT CCTACCTGGC CCGCGCCGGC AAACACGAGT TCCTCAACCT TGTGCCTAAC 
ATAGAGGAAA TGAGGCCTGG GTCTGTGGTG TCCAAGAAGG GCATCTTGAA TTTCATGGAG 
CCCCGCTCGA ACACCTGGGT GAAGCACTTT GTGGTGGTGC GTCGGCCGTA TGTGTTCATC 
TACAACAATG ACAAGGACCC TGTGGAGAGA GGAGTGCTGA ACTTATCCAC TGCACAGGTG 
GAGTACAGTG AAGACCAGCA GGCCATGCTC AAGACGCCTC ATACATTTGC CATGTGCACC 
AAACACAGAG GAATCCTGCT TCAGGCCAAC AATGACAAAG ACATGAACGA CTGGCTGTAT 
GCCTTTAACC CACTGCTAGC AGGAACAATA AGGTCGAAGC TTGCAAGGAG ACGGTCTGGT 
GTGATGAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296812.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295087.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.

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CloneID	ODa40261
Clone ID Related Accession (Same CDS sequence)	XM_009296812.2 , XM_009296812.3
Accession Version	XM_009296812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGCCT TTGTATTGAT 
AGCGATATTT CTAGGACGTT CTACCGGTTT GAGGCCGTGT GGGACAGTTC TCTGCACAAC 
TCTCTCCTAC TGAACCGCGT CACTCCATAC GGAGAGAAGA TCTACATGAC CCTGTCTGCC 
TATCTGGAGC TCGATCACTG CATCCAGCCT GCTATCATCA CCAAGGACAT CTGTATGGTC 
TTCTACTCCA GAGATGCCAA GATCTCGCCT CCTCGCTCGC TGAGAAACTT GTTTGGCAGC 
GGCTACTCCA AAACCCCGGA CTGTAATAAA GTGACTGGAG TCTATGAGCT GAGCTTGTGC 
AAGATAGCAG ACACCGGAAG CCCAGGTATG CAGCGGCGCA GACGGAAGGT TCTGGACACG 
TCAGTGGCAT ATGTGCGTGG AGAGGAGAAT CTGGCCGGCT GGAGACCCCG AGGAGACAGT 
CTGATCCTGG AGCACCAGTG GGAGCTGGAC AAAATGGAGC AGCTGCATGA GGTGGAAAAG 
ACGCGTCACT TGCTTCTGCT GCGTGAGAAG CTGGGAGAAA CACCTCCACC TAAATCTCTG 
AGCGAGTCTC TGTCCCCCAG CCTGAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCCCAGATCT CCTCCACAAC TTTCGAGAGT GCCATCACCC CCAGCGAAAG CAGCGGCTAT 
GACTCCACGG ACATCGAGAG CTTGGTGGAT CGAGAGAAGG AGCTGGCCAC TAAGTGCCTC 
CGTCTCCTGA CACACACTTT CAACAGTGAA TACAACCAAA TGTGCAACAG CATTAGTGAC 
TGCAAGTTTT CAGATATCTC TCCTATGGGT CGGGATCCAT CAGTCAGTAG TTTCAGCAGT 
GCCACCCTCA CACCCTCCTC CACCTGCCCC TCGCTCTCAG ACTCCAGGAC CCCTGAAGCA 
AACTCTCGTG CAACCAGCCC CTCAGGCTCA GACTATGAGA ACTTCCCCAT GGTGCCCATT 
CTGGAGACGT CCTACCTGGC CCGCGCCGGC AAACACGAGT TCCTCAACCT TGTGCCTAAC 
ATAGAGGAAA TGAGGCCTGG GTCTGTGGTG TCCAAGAAGG GCATCTTGAA TTTCATGGAG 
CCCCGCTCGA ACACCTGGGT GAAGCACTTT GTGGTGGTGC GTCGGCCGTA TGTGTTCATC 
TACAACAATG ACAAGGACCC TGTGGAGAGA GGAGTGCTGA ACTTATCCAC TGCACAGGTG 
GAGTACAGTG AAGACCAGCA GGCCATGCTC AAGACGCCTC ATACATTTGC CATGTGCACC 
AAACACAGAG GAATCCTGCT TCAGGCCAAC AATGACAAAG ACATGAACGA CTGGCTGTAT 
GCCTTTAACC CACTGCTAGC AGGAACAATA AGGTCGAAGC TTGCAAGGAG ACGGTCTGGT 
GTGATGAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295087.1
CDS	320..6094
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296812.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296812.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGCCT TTGTATTGAT 
AGCGATATTT CTAGGACGTT CTACCGGTTT GAGGCCGTGT GGGACAGTTC TCTGCACAAC 
TCTCTCCTAC TGAACCGCGT CACTCCATAC GGAGAGAAGA TCTACATGAC CCTGTCTGCC 
TATCTGGAGC TCGATCACTG CATCCAGCCT GCTATCATCA CCAAGGACAT CTGTATGGTC 
TTCTACTCCA GAGATGCCAA GATCTCGCCT CCTCGCTCGC TGAGAAACTT GTTTGGCAGC 
GGCTACTCCA AAACCCCGGA CTGTAATAAA GTGACTGGAG TCTATGAGCT GAGCTTGTGC 
AAGATAGCAG ACACCGGAAG CCCAGGTATG CAGCGGCGCA GACGGAAGGT TCTGGACACG 
TCAGTGGCAT ATGTGCGTGG AGAGGAGAAT CTGGCCGGCT GGAGACCCCG AGGAGACAGT 
CTGATCCTGG AGCACCAGTG GGAGCTGGAC AAAATGGAGC AGCTGCATGA GGTGGAAAAG 
ACGCGTCACT TGCTTCTGCT GCGTGAGAAG CTGGGAGAAA CACCTCCACC TAAATCTCTG 
AGCGAGTCTC TGTCCCCCAG CCTGAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCCCAGATCT CCTCCACAAC TTTCGAGAGT GCCATCACCC CCAGCGAAAG CAGCGGCTAT 
GACTCCACGG ACATCGAGAG CTTGGTGGAT CGAGAGAAGG AGCTGGCCAC TAAGTGCCTC 
CGTCTCCTGA CACACACTTT CAACAGTGAA TACAACCAAA TGTGCAACAG CATTAGTGAC 
TGCAAGTTTT CAGATATCTC TCCTATGGGT CGGGATCCAT CAGTCAGTAG TTTCAGCAGT 
GCCACCCTCA CACCCTCCTC CACCTGCCCC TCGCTCTCAG ACTCCAGGAC CCCTGAAGCA 
AACTCTCGTG CAACCAGCCC CTCAGGCTCA GACTATGAGA ACTTCCCCAT GGTGCCCATT 
CTGGAGACGT CCTACCTGGC CCGCGCCGGC AAACACGAGT TCCTCAACCT TGTGCCTAAC 
ATAGAGGAAA TGAGGCCTGG GTCTGTGGTG TCCAAGAAGG GCATCTTGAA TTTCATGGAG 
CCCCGCTCGA ACACCTGGGT GAAGCACTTT GTGGTGGTGC GTCGGCCGTA TGTGTTCATC 
TACAACAATG ACAAGGACCC TGTGGAGAGA GGAGTGCTGA ACTTATCCAC TGCACAGGTG 
GAGTACAGTG AAGACCAGCA GGCCATGCTC AAGACGCCTC ATACATTTGC CATGTGCACC 
AAACACAGAG GAATCCTGCT TCAGGCCAAC AATGACAAAG ACATGAACGA CTGGCTGTAT 
GCCTTTAACC CACTGCTAGC AGGAACAATA AGGTCGAAGC TTGCAAGGAG ACGGTCTGGT 
GTGATGAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296813.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295088.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.

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CloneID	ODa40262
Clone ID Related Accession (Same CDS sequence)	XM_009296813.2 , XM_009296813.3
Accession Version	XM_009296813.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TGTGACGATA TTAACGATGT TAAAACGCTG CTAAGTTCAC AAACTGAAGC AATGCTCTAT 
CGGAGAAAAG CACCCATGGG CTGTCTGACA GCCTCTCCAT CTTCAGGCTC TCTCTGCAGC 
CAGGCAGGCC TACAGTCCGT CTCCAGCATC CAGGAGCGCA TCATGTCCAC GCCAGGCGGA 
GAAGAGGCCA TCGAGAGGCT CAAGGAATCA GAAAAGATCA TAGCCGAGCT GAATGAGACC 
TGGGAAGAGA AGCTGAGGAA GACCGAGGCC ATCCGTATGG AGAGAGAGGC GCTGCTGGCA 
GAGATGGGAG TTGCCATACG GGAGGACGGG GGAACCCTGG GCGTCTTCTC TCCTAAAAAG 
TTTGGTCCTG ATAGACCAAC GTCACTGTCC ATTGAGGACT TTAGTGTTTA TTCTTCCAAT 
CAGCTTTTCG TTGAAAGGCC CTGTGAAGTC TATACTGACT TTAATGGGGT GTTTTCACCC 
AAAAAGACGC CTCACCTGGT GAACCTGAAC GAGGATCCTT TGATGTCAGA ATGTCTGCTC 
TATTATATCA AAGATGGAAT AACAAGGGTG GGCCAGGCAG ATGCTGAAAG AAGACAGGAT 
ATCGTTCTAA GTGGAGCTCA CATTAAAGAG GAGCACTGTA TTTTCCGCAG TGAGAGGAAT 
GCCAATGGAC ATGTGATTGT CACGTTGGAG CCATGTGAGG GATCAGAAAC ATATGTAAAT 
GGTAAACGTG TGAATTCTGC GGTCCAGCTG CGCTCAGGTA ACCGCATTAT CATGGGAAAG 
AACCACGTGT TCCGGTTTAA CCACCCTGAG CAGGCTCGCG CTGAGCGTGA GAAGACCCCG 
TCAGCAGAGA CACCTGTAGA ACCTGTTGAC TGGACTTTTG CCCAAAGAGA ACTGCTGGAG 
AAACAGGGTA TCGACATGAA GCAAGAGATG GAGAAGAGGC TTACAGAAAT GGAGATCCTG 
TACAAAAAAG AGAAGGAGGA AGCGGACCAG CTACTGGAGC AACAGAGACT GGTGTATGAG 
AGCAAACTGC AAGAGCTGCA GAAACAAGTG GAGACTCGTT CTTTGGCTGC AGAGACTCCA 
GATGAGGAGG AAGAAGAAGA GGAGGAGGAT GAAGTGCCCT GGACCCAGCA TAAATATGAG 
CTGGCTCAGT GGGCTTTCAG GAAGTGGAGA TACCACCAGT TCACCTCCCT CAGAGACCAA 
TTATGGGGCA ATGCTGTGTA CCTCAAAGAA GCCAATGCCA TCAGTGTGGA GCTCAAGAAA 
AAGGTCCAGT TCCAGTTCGT CTTGCTCACA GACACCCTCT ATTCTCCACT GCCCCCGGAG 
CTGCTGCCTC CAGAGCCAGA GAAAGAGCGA GACGCCAGGC CTTTTCCGAG AACAGTGGTG 
GCTGTGGAGG TTCAGGACCT GAAGAATGGA GCCACTCACT ACTGGTCCCT GGAGAAGCTG 
AAGCAGAGGC TGGACCAGAT GCGAGAGATG TACGACCGTG CTGGCGAAAT GGCCTCGTCC 
AATCAGGGAG ATGATGGGGA GGGTGCGTTG ACCGGCAGTG ACCCGTTCTA TGACCGATTT 
CATTGGTTTA AGCTGGTTGG GAGCTCACCT ATTTTTCATG GCTGTGTGAA CGAGCGTCTG 
GCCGACCGCA CACCATCGCC CACCTTCTCC ACCACCGATT CGGAGATCAC TGAGCTGGCC 
GACGAGCGAC AGAGCGAGAT GGAGGACTTT ATGGATGACG AGGCTTTCGT GGATGACACC 
AGCTCAGATG CTGGAACCGA GGAGGGCTCG GACATCTTCA GTGATGGCCA GGACCCCTTC 
TATGATCGTT CTCCATGGTT CATCCTGGTG GGCAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295088.1
CDS	319..6075
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296813.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296813.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TGTGACGATA TTAACGATGT TAAAACGCTG CTAAGTTCAC AAACTGAAGC AATGCTCTAT 
CGGAGAAAAG CACCCATGGG CTGTCTGACA GCCTCTCCAT CTTCAGGCTC TCTCTGCAGC 
CAGGCAGGCC TACAGTCCGT CTCCAGCATC CAGGAGCGCA TCATGTCCAC GCCAGGCGGA 
GAAGAGGCCA TCGAGAGGCT CAAGGAATCA GAAAAGATCA TAGCCGAGCT GAATGAGACC 
TGGGAAGAGA AGCTGAGGAA GACCGAGGCC ATCCGTATGG AGAGAGAGGC GCTGCTGGCA 
GAGATGGGAG TTGCCATACG GGAGGACGGG GGAACCCTGG GCGTCTTCTC TCCTAAAAAG 
TTTGGTCCTG ATAGACCAAC GTCACTGTCC ATTGAGGACT TTAGTGTTTA TTCTTCCAAT 
CAGCTTTTCG TTGAAAGGCC CTGTGAAGTC TATACTGACT TTAATGGGGT GTTTTCACCC 
AAAAAGACGC CTCACCTGGT GAACCTGAAC GAGGATCCTT TGATGTCAGA ATGTCTGCTC 
TATTATATCA AAGATGGAAT AACAAGGGTG GGCCAGGCAG ATGCTGAAAG AAGACAGGAT 
ATCGTTCTAA GTGGAGCTCA CATTAAAGAG GAGCACTGTA TTTTCCGCAG TGAGAGGAAT 
GCCAATGGAC ATGTGATTGT CACGTTGGAG CCATGTGAGG GATCAGAAAC ATATGTAAAT 
GGTAAACGTG TGAATTCTGC GGTCCAGCTG CGCTCAGGTA ACCGCATTAT CATGGGAAAG 
AACCACGTGT TCCGGTTTAA CCACCCTGAG CAGGCTCGCG CTGAGCGTGA GAAGACCCCG 
TCAGCAGAGA CACCTGTAGA ACCTGTTGAC TGGACTTTTG CCCAAAGAGA ACTGCTGGAG 
AAACAGGGTA TCGACATGAA GCAAGAGATG GAGAAGAGGC TTACAGAAAT GGAGATCCTG 
TACAAAAAAG AGAAGGAGGA AGCGGACCAG CTACTGGAGC AACAGAGACT GGTGTATGAG 
AGCAAACTGC AAGAGCTGCA GAAACAAGTG GAGACTCGTT CTTTGGCTGC AGAGACTCCA 
GATGAGGAGG AAGAAGAAGA GGAGGAGGAT GAAGTGCCCT GGACCCAGCA TAAATATGAG 
CTGGCTCAGT GGGCTTTCAG GAAGTGGAGA TACCACCAGT TCACCTCCCT CAGAGACCAA 
TTATGGGGCA ATGCTGTGTA CCTCAAAGAA GCCAATGCCA TCAGTGTGGA GCTCAAGAAA 
AAGGTCCAGT TCCAGTTCGT CTTGCTCACA GACACCCTCT ATTCTCCACT GCCCCCGGAG 
CTGCTGCCTC CAGAGCCAGA GAAAGAGCGA GACGCCAGGC CTTTTCCGAG AACAGTGGTG 
GCTGTGGAGG TTCAGGACCT GAAGAATGGA GCCACTCACT ACTGGTCCCT GGAGAAGCTG 
AAGCAGAGGC TGGACCAGAT GCGAGAGATG TACGACCGTG CTGGCGAAAT GGCCTCGTCC 
AATCAGGGAG ATGATGGGGA GGGTGCGTTG ACCGGCAGTG ACCCGTTCTA TGACCGATTT 
CATTGGTTTA AGCTGGTTGG GAGCTCACCT ATTTTTCATG GCTGTGTGAA CGAGCGTCTG 
GCCGACCGCA CACCATCGCC CACCTTCTCC ACCACCGATT CGGAGATCAC TGAGCTGGCC 
GACGAGCGAC AGAGCGAGAT GGAGGACTTT ATGGATGACG AGGCTTTCGT GGATGACACC 
AGCTCAGATG CTGGAACCGA GGAGGGCTCG GACATCTTCA GTGATGGCCA GGACCCCTTC 
TATGATCGTT CTCCATGGTT CATCCTGGTG GGCAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296813.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295088.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.

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CloneID	ODa40262
Clone ID Related Accession (Same CDS sequence)	XM_009296813.2 , XM_009296813.3
Accession Version	XM_009296813.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TGTGACGATA TTAACGATGT TAAAACGCTG CTAAGTTCAC AAACTGAAGC AATGCTCTAT 
CGGAGAAAAG CACCCATGGG CTGTCTGACA GCCTCTCCAT CTTCAGGCTC TCTCTGCAGC 
CAGGCAGGCC TACAGTCCGT CTCCAGCATC CAGGAGCGCA TCATGTCCAC GCCAGGCGGA 
GAAGAGGCCA TCGAGAGGCT CAAGGAATCA GAAAAGATCA TAGCCGAGCT GAATGAGACC 
TGGGAAGAGA AGCTGAGGAA GACCGAGGCC ATCCGTATGG AGAGAGAGGC GCTGCTGGCA 
GAGATGGGAG TTGCCATACG GGAGGACGGG GGAACCCTGG GCGTCTTCTC TCCTAAAAAG 
TTTGGTCCTG ATAGACCAAC GTCACTGTCC ATTGAGGACT TTAGTGTTTA TTCTTCCAAT 
CAGCTTTTCG TTGAAAGGCC CTGTGAAGTC TATACTGACT TTAATGGGGT GTTTTCACCC 
AAAAAGACGC CTCACCTGGT GAACCTGAAC GAGGATCCTT TGATGTCAGA ATGTCTGCTC 
TATTATATCA AAGATGGAAT AACAAGGGTG GGCCAGGCAG ATGCTGAAAG AAGACAGGAT 
ATCGTTCTAA GTGGAGCTCA CATTAAAGAG GAGCACTGTA TTTTCCGCAG TGAGAGGAAT 
GCCAATGGAC ATGTGATTGT CACGTTGGAG CCATGTGAGG GATCAGAAAC ATATGTAAAT 
GGTAAACGTG TGAATTCTGC GGTCCAGCTG CGCTCAGGTA ACCGCATTAT CATGGGAAAG 
AACCACGTGT TCCGGTTTAA CCACCCTGAG CAGGCTCGCG CTGAGCGTGA GAAGACCCCG 
TCAGCAGAGA CACCTGTAGA ACCTGTTGAC TGGACTTTTG CCCAAAGAGA ACTGCTGGAG 
AAACAGGGTA TCGACATGAA GCAAGAGATG GAGAAGAGGC TTACAGAAAT GGAGATCCTG 
TACAAAAAAG AGAAGGAGGA AGCGGACCAG CTACTGGAGC AACAGAGACT GGTGTATGAG 
AGCAAACTGC AAGAGCTGCA GAAACAAGTG GAGACTCGTT CTTTGGCTGC AGAGACTCCA 
GATGAGGAGG AAGAAGAAGA GGAGGAGGAT GAAGTGCCCT GGACCCAGCA TAAATATGAG 
CTGGCTCAGT GGGCTTTCAG GAAGTGGAGA TACCACCAGT TCACCTCCCT CAGAGACCAA 
TTATGGGGCA ATGCTGTGTA CCTCAAAGAA GCCAATGCCA TCAGTGTGGA GCTCAAGAAA 
AAGGTCCAGT TCCAGTTCGT CTTGCTCACA GACACCCTCT ATTCTCCACT GCCCCCGGAG 
CTGCTGCCTC CAGAGCCAGA GAAAGAGCGA GACGCCAGGC CTTTTCCGAG AACAGTGGTG 
GCTGTGGAGG TTCAGGACCT GAAGAATGGA GCCACTCACT ACTGGTCCCT GGAGAAGCTG 
AAGCAGAGGC TGGACCAGAT GCGAGAGATG TACGACCGTG CTGGCGAAAT GGCCTCGTCC 
AATCAGGGAG ATGATGGGGA GGGTGCGTTG ACCGGCAGTG ACCCGTTCTA TGACCGATTT 
CATTGGTTTA AGCTGGTTGG GAGCTCACCT ATTTTTCATG GCTGTGTGAA CGAGCGTCTG 
GCCGACCGCA CACCATCGCC CACCTTCTCC ACCACCGATT CGGAGATCAC TGAGCTGGCC 
GACGAGCGAC AGAGCGAGAT GGAGGACTTT ATGGATGACG AGGCTTTCGT GGATGACACC 
AGCTCAGATG CTGGAACCGA GGAGGGCTCG GACATCTTCA GTGATGGCCA GGACCCCTTC 
TATGATCGTT CTCCATGGTT CATCCTGGTG GGCAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295088.1
CDS	320..6076
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296813.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296813.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAT CGAGCCAATG GGGGATGATT GCCTTGGAAG TGGAAGCAAA 
TGTGACGATA TTAACGATGT TAAAACGCTG CTAAGTTCAC AAACTGAAGC AATGCTCTAT 
CGGAGAAAAG CACCCATGGG CTGTCTGACA GCCTCTCCAT CTTCAGGCTC TCTCTGCAGC 
CAGGCAGGCC TACAGTCCGT CTCCAGCATC CAGGAGCGCA TCATGTCCAC GCCAGGCGGA 
GAAGAGGCCA TCGAGAGGCT CAAGGAATCA GAAAAGATCA TAGCCGAGCT GAATGAGACC 
TGGGAAGAGA AGCTGAGGAA GACCGAGGCC ATCCGTATGG AGAGAGAGGC GCTGCTGGCA 
GAGATGGGAG TTGCCATACG GGAGGACGGG GGAACCCTGG GCGTCTTCTC TCCTAAAAAG 
TTTGGTCCTG ATAGACCAAC GTCACTGTCC ATTGAGGACT TTAGTGTTTA TTCTTCCAAT 
CAGCTTTTCG TTGAAAGGCC CTGTGAAGTC TATACTGACT TTAATGGGGT GTTTTCACCC 
AAAAAGACGC CTCACCTGGT GAACCTGAAC GAGGATCCTT TGATGTCAGA ATGTCTGCTC 
TATTATATCA AAGATGGAAT AACAAGGGTG GGCCAGGCAG ATGCTGAAAG AAGACAGGAT 
ATCGTTCTAA GTGGAGCTCA CATTAAAGAG GAGCACTGTA TTTTCCGCAG TGAGAGGAAT 
GCCAATGGAC ATGTGATTGT CACGTTGGAG CCATGTGAGG GATCAGAAAC ATATGTAAAT 
GGTAAACGTG TGAATTCTGC GGTCCAGCTG CGCTCAGGTA ACCGCATTAT CATGGGAAAG 
AACCACGTGT TCCGGTTTAA CCACCCTGAG CAGGCTCGCG CTGAGCGTGA GAAGACCCCG 
TCAGCAGAGA CACCTGTAGA ACCTGTTGAC TGGACTTTTG CCCAAAGAGA ACTGCTGGAG 
AAACAGGGTA TCGACATGAA GCAAGAGATG GAGAAGAGGC TTACAGAAAT GGAGATCCTG 
TACAAAAAAG AGAAGGAGGA AGCGGACCAG CTACTGGAGC AACAGAGACT GGTGTATGAG 
AGCAAACTGC AAGAGCTGCA GAAACAAGTG GAGACTCGTT CTTTGGCTGC AGAGACTCCA 
GATGAGGAGG AAGAAGAAGA GGAGGAGGAT GAAGTGCCCT GGACCCAGCA TAAATATGAG 
CTGGCTCAGT GGGCTTTCAG GAAGTGGAGA TACCACCAGT TCACCTCCCT CAGAGACCAA 
TTATGGGGCA ATGCTGTGTA CCTCAAAGAA GCCAATGCCA TCAGTGTGGA GCTCAAGAAA 
AAGGTCCAGT TCCAGTTCGT CTTGCTCACA GACACCCTCT ATTCTCCACT GCCCCCGGAG 
CTGCTGCCTC CAGAGCCAGA GAAAGAGCGA GACGCCAGGC CTTTTCCGAG AACAGTGGTG 
GCTGTGGAGG TTCAGGACCT GAAGAATGGA GCCACTCACT ACTGGTCCCT GGAGAAGCTG 
AAGCAGAGGC TGGACCAGAT GCGAGAGATG TACGACCGTG CTGGCGAAAT GGCCTCGTCC 
AATCAGGGAG ATGATGGGGA GGGTGCGTTG ACCGGCAGTG ACCCGTTCTA TGACCGATTT 
CATTGGTTTA AGCTGGTTGG GAGCTCACCT ATTTTTCATG GCTGTGTGAA CGAGCGTCTG 
GCCGACCGCA CACCATCGCC CACCTTCTCC ACCACCGATT CGGAGATCAC TGAGCTGGCC 
GACGAGCGAC AGAGCGAGAT GGAGGACTTT ATGGATGACG AGGCTTTCGT GGATGACACC 
AGCTCAGATG CTGGAACCGA GGAGGGCTCG GACATCTTCA GTGATGGCCA GGACCCCTTC 
TATGATCGTT CTCCATGGTT CATCCTGGTG GGCAGAGCCT TCGTGTACCT GAGTAACCTG 
CTGTACCCTG TTCCTCTGGT GCATCGAGTC GCTGTAGTGA CAGAGAAAGG AGATGTGCGA 
GGATTTCTGC GTGTGGGGGT CCAGGCCATC GCCGCGGATG AAGAGGCACC TGATTATGGG 
TCTGGTGTGC GTCAGTCAGG AACTGCTAAG ATTTCCTTTG ATAATGAGTA TTTTAAAAAG 
AATGACTTCA CCTCAGTGGC CATGACCCGC TCTGGTCTCT CTCTGGAGGA GCTGCGCATT 
GTAGAAGGTC AAGGTCAGAG TTCAGAGGTC ATTACGCCTT CAGAGGAGAT GAACAGAATC 
AATGAAATGG ACCTAAAGCT GGGGAACGTG GACACAAAAC TGGGAGACGG TTTGGCCGAG 
CATCTGGAAG TGGGCAGCAT TTTCACTTTC CGCGTCACCG TTCTGCAGGC AAGCGGCATC 
CCGGCCGAAT ACGCAGACAT CTTCTGCCAG TTCAATTTCC TCCACCGTCA CGATGAGGCG 
TTCAGCACTG AGCCTCTGAA GAACACTGGC AGAGGAGCCC CGCTGGGCTT TTACCACGTA 
CAGAATATCT CAGTGGAGGT GACAGAGTCC TTCATTGAGT ACATAAAGAC CAAGCCAATT 
GTATTTGAAG TGTTTGGCCA TTACCAGCAA CACCCACTGC ACATTCATGG ACAGGATGTG 
GTCAGCCCTT CTCAGCCTTC CAGAAAGTAC TATCCTCCAC CAATGCCTCT GTCTAAACCA 
GATCACGCTC GTAAGATTGA GCTTATCCGT TACCTGATGA GCGGGTATGT TCATGGTCAG 
GTGTTGGACA CATTGTCAGA GCATGCCAAC GCTTTGGCCT CCACCGCCGT GGCCAGTCTG 
GAGGAAGGGG CATATGAGCT GTGCCACTTC CCCTTCCTCC CCATACCCAA CTGCCCCGTC 
TTCAGAGTGC CCCGACACGA CGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGC CTTTGTATTG ATAGCGATAT TTCTAGGACG 
TTCTACCGGT TTGAGGCCGT GTGGGACAGT TCTCTGCACA ACTCTCTCCT ACTGAACCGC 
GTCACTCCAT ACGGAGAGAA GATCTACATG ACCCTGTCTG CCTATCTGGA GCTCGATCAC 
TGCATCCAGC CTGCTATCAT CACCAAGGAC ATCTGTATGG TCTTCTACTC CAGAGATGCC 
AAGATCTCGC CTCCTCGCTC GCTGAGAAAC TTGTTTGGCA GCGGCTACTC CAAAACCCCG 
GACTGTAATA AAGTGACTGG AGTCTATGAG CTGAGCTTGT GCAAGATAGC AGACACCGGA 
AGCCCAGGTA TGCAGCGGCG CAGACGGAAG GTTCTGGACA CGTCAGTGGC ATATGTGCGT 
GGAGAGGAGA ATCTGGCCGG CTGGAGACCC CGAGGAGACA GTCTGATCCT GGAGCACCAG 
TGGGAGCTGG ACAAAATGGA GCAGCTGCAT GAGGTGGAAA AGACGCGTCA CTTGCTTCTG 
CTGCGTGAGA AGCTGGGAGA AACACCTCCA CCTAAATCTC TGAGCGAGTC TCTGTCCCCC 
AGCCTGAGCA GCGGGACCCT CAGCACCTCC ACCAGCATCT CCTCCCAGAT CTCCTCCACA 
ACTTTCGAGA GTGCCATCAC CCCCAGCGAA AGCAGCGGCT ATGACTCCAC GGACATCGAG 
AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC ACTAAGTGCC TCCGTCTCCT GACACACACT 
TTCAACAGTG AATACAACCA AATGTGCAAC AGCATTAGTG ACTGCAAGTT TTCAGATATC 
TCTCCTATGG GTCGGGATCC ATCAGTCAGT AGTTTCAGCA GTGCCACCCT CACACCCTCC 
TCCACCTGCC CCTCGCTCTC AGACTCCAGG ACCCCTGAAG CAAACTCTCG TGCAACCAGC 
CCCTCAGGCT CAGACTATGA GAACTTCCCC ATGGTGCCCA TTCTGGAGAC GTCCTACCTG 
GCCCGCGCCG GCAAACACGA GTTCCTCAAC CTTGTGCCTA ACATAGAGGA AATGAGGCCT 
GGGTCTGTGG TGTCCAAGAA GGGCATCTTG AATTTCATGG AGCCCCGCTC GAACACCTGG 
GTGAAGCACT TTGTGGTGGT GCGTCGGCCG TATGTGTTCA TCTACAACAA TGACAAGGAC 
CCTGTGGAGA GAGGAGTGCT GAACTTATCC ACTGCACAGG TGGAGTACAG TGAAGACCAG 
CAGGCCATGC TCAAGACGCC TCATACATTT GCCATGTGCA CCAAACACAG AGGAATCCTG 
CTTCAGGCCA ACAATGACAA AGACATGAAC GACTGGCTGT ATGCCTTTAA CCCACTGCTA 
GCAGGAACAA TAAGGTCGAA GCTTGCAAGG AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296814.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295089.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.

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CloneID	ODa40263
Clone ID Related Accession (Same CDS sequence)	XM_009296814.3 , XM_009296814.2
Accession Version	XM_009296814.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295089.1
CDS	320..6067
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296814.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296814.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296814.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295089.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.

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CloneID	ODa40263
Clone ID Related Accession (Same CDS sequence)	XM_009296814.3 , XM_009296814.2
Accession Version	XM_009296814.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295089.1
CDS	319..6066
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296814.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296814.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGA TCACGCTCGT AAGATTGAGC TTATCCGTTA CCTGATGAGC 
GGGTATGTTC ATGGTCAGGT GTTGGACACA TTGTCAGAGC ATGCCAACGC TTTGGCCTCC 
ACCGCCGTGG CCAGTCTGGA GGAAGGGGCA TATGAGCTGT GCCACTTCCC CTTCCTCCCC 
ATACCCAACT GCCCCGTCTT CAGAGTGCCC CGACACGACG TGCCAGCCAC CAAACTGAAC 
ACCATCACCA AGTCCAACCT GGGTCAGTGT GTGAGCAAGT ACGACTTGCT GGCCTGGTTT 
GAGATCAGCG AACTGGAGCC CACTGGAGAG TATATTCCAG CAGTAGTGGA TCATACAGGA 
GGTCTTCCCT GTCATGGCAC ATACCTGCTG CACCAGGGCA TCCAGCGCAG AATAACAGTG 
ACTCTGATCC ATGAGAAGGG AAGTGAGCTG CACTGGAAGG ACGTCCGAGA GCTGGTGGTC 
GGTCGAATCA GGAATAAGCC AGAAGTGGAT GATTCAGCCG CAGACGCCGT CTTGTCTCTC 
AACATCATCT CAGCTAAAAA CCTCAAATCC TCCCACAACT CCAGCAGGAC GTTCTACCGG 
TTTGAGGCCG TGTGGGACAG TTCTCTGCAC AACTCTCTCC TACTGAACCG CGTCACTCCA 
TACGGAGAGA AGATCTACAT GACCCTGTCT GCCTATCTGG AGCTCGATCA CTGCATCCAG 
CCTGCTATCA TCACCAAGGA CATCTGTATG GTCTTCTACT CCAGAGATGC CAAGATCTCG 
CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC AGCGGCTACT CCAAAACCCC GGACTGTAAT 
AAAGTGACTG GAGTCTATGA GCTGAGCTTG TGCAAGATAG CAGACACCGG AAGCCCAGGT 
ATGCAGCGGC GCAGACGGAA GGTTCTGGAC ACGTCAGTGG CATATGTGCG TGGAGAGGAG 
AATCTGGCCG GCTGGAGACC CCGAGGAGAC AGTCTGATCC TGGAGCACCA GTGGGAGCTG 
GACAAAATGG AGCAGCTGCA TGAGGTGGAA AAGACGCGTC ACTTGCTTCT GCTGCGTGAG 
AAGCTGGGAG AAACACCTCC ACCTAAATCT CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC 
AGCGGGACCC TCAGCACCTC CACCAGCATC TCCTCCCAGA TCTCCTCCAC AACTTTCGAG 
AGTGCCATCA CCCCCAGCGA AAGCAGCGGC TATGACTCCA CGGACATCGA GAGCTTGGTG 
GATCGAGAGA AGGAGCTGGC CACTAAGTGC CTCCGTCTCC TGACACACAC TTTCAACAGT 
GAATACAACC AAATGTGCAA CAGCATTAGT GACTGCAAGT TTTCAGATAT CTCTCCTATG 
GGTCGGGATC CATCAGTCAG TAGTTTCAGC AGTGCCACCC TCACACCCTC CTCCACCTGC 
CCCTCGCTCT CAGACTCCAG GACCCCTGAA GCAAACTCTC GTGCAACCAG CCCCTCAGGC 
TCAGACTATG AGAACTTCCC CATGGTGCCC ATTCTGGAGA CGTCCTACCT GGCCCGCGCC 
GGCAAACACG AGTTCCTCAA CCTTGTGCCT AACATAGAGG AAATGAGGCC TGGGTCTGTG 
GTGTCCAAGA AGGGCATCTT GAATTTCATG GAGCCCCGCT CGAACACCTG GGTGAAGCAC 
TTTGTGGTGG TGCGTCGGCC GTATGTGTTC ATCTACAACA ATGACAAGGA CCCTGTGGAG 
AGAGGAGTGC TGAACTTATC CACTGCACAG GTGGAGTACA GTGAAGACCA GCAGGCCATG 
CTCAAGACGC CTCATACATT TGCCATGTGC ACCAAACACA GAGGAATCCT GCTTCAGGCC 
AACAATGACA AAGACATGAA CGACTGGCTG TATGCCTTTA ACCCACTGCT AGCAGGAACA 
ATAAGGTCGA AGCTTGCAAG GAGACGGTCT GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296815.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295090.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.

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CloneID	ODa40264
Clone ID Related Accession (Same CDS sequence)	XM_009296815.3 , XM_009296815.2
Accession Version	XM_009296815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAGTG ACGATATTAA CGATGTTAAA 
ACGCTGCTAA GTTCACAAAC TGAAGCAATG CTCTATCGGA GAAAAGCACC CATGGGCTGT 
CTGACAGCCT CTCCATCTTC AGGCTCTCTC TGCAGCCAGG CAGGCCTACA GTCCGTCTCC 
AGCATCCAGG AGCGCATCAT GTCCACGCCA GGCGGAGAAG AGGCCATCGA GAGGCTCAAG 
GAATCAGAAA AGATCATAGC CGAGCTGAAT GAGACCTGGG AAGAGAAGCT GAGGAAGACC 
GAGGCCATCC GTATGGAGAG AGAGGCGCTG CTGGCAGAGA TGGGAGTTGC CATACGGGAG 
GACGGGGGAA CCCTGGGCGT CTTCTCTCCT AAAAAGTTTG GTCCTGATAG ACCAACGTCA 
CTGTCCATTG AGGACTTTAG TGTTTATTCT TCCAATCAGC TTTTCGTTGA AAGGCCCTGT 
GAAGTCTATA CTGACTTTAA TGGGGTGTTT TCACCCAAAA AGACGCCTCA CCTGGTGAAC 
CTGAACGAGG ATCCTTTGAT GTCAGAATGT CTGCTCTATT ATATCAAAGA TGGAATAACA 
AGGGTGGGCC AGGCAGATGC TGAAAGAAGA CAGGATATCG TTCTAAGTGG AGCTCACATT 
AAAGAGGAGC ACTGTATTTT CCGCAGTGAG AGGAATGCCA ATGGACATGT GATTGTCACG 
TTGGAGCCAT GTGAGGGATC AGAAACATAT GTAAATGGTA AACGTGTGAA TTCTGCGGTC 
CAGCTGCGCT CAGGTAACCG CATTATCATG GGAAAGAACC ACGTGTTCCG GTTTAACCAC 
CCTGAGCAGG CTCGCGCTGA GCGTGAGAAG ACCCCGTCAG CAGAGACACC TGTAGAACCT 
GTTGACTGGA CTTTTGCCCA AAGAGAACTG CTGGAGAAAC AGGGTATCGA CATGAAGCAA 
GAGATGGAGA AGAGGCTTAC AGAAATGGAG ATCCTGTACA AAAAAGAGAA GGAGGAAGCG 
GACCAGCTAC TGGAGCAACA GAGACTGGTG TATGAGAGCA AACTGCAAGA GCTGCAGAAA 
CAAGTGGAGA CTCGTTCTTT GGCTGCAGAG ACTCCAGATG AGGAGGAAGA AGAAGAGGAG 
GAGGATGAAG TGCCCTGGAC CCAGCATAAA TATGAGCTGG CTCAGTGGGC TTTCAGGAAG 
TGGAGATACC ACCAGTTCAC CTCCCTCAGA GACCAATTAT GGGGCAATGC TGTGTACCTC 
AAAGAAGCCA ATGCCATCAG TGTGGAGCTC AAGAAAAAGG TCCAGTTCCA GTTCGTCTTG 
CTCACAGACA CCCTCTATTC TCCACTGCCC CCGGAGCTGC TGCCTCCAGA GCCAGAGAAA 
GAGCGAGACG CCAGGCCTTT TCCGAGAACA GTGGTGGCTG TGGAGGTTCA GGACCTGAAG 
AATGGAGCCA CTCACTACTG GTCCCTGGAG AAGCTGAAGC AGAGGCTGGA CCAGATGCGA 
GAGATGTACG ACCGTGCTGG CGAAATGGCC TCGTCCAATC AGGGAGATGA TGGGGAGGGT 
GCGTTGACCG GCAGTGACCC GTTCTATGAC CGATTTCATT GGTTTAAGCT GGTTGGGAGC 
TCACCTATTT TTCATGGCTG TGTGAACGAG CGTCTGGCCG ACCGCACACC ATCGCCCACC 
TTCTCCACCA CCGATTCGGA GATCACTGAG CTGGCCGACG AGCGACAGAG CGAGATGGAG 
GACTTTATGG ATGACGAGGC TTTCGTGGAT GACACCAGCT CAGATGCTGG AACCGAGGAG 
GGCTCGGACA TCTTCAGTGA TGGCCAGGAC CCCTTCTATG ATCGTTCTCC ATGGTTCATC 
CTGGTGGGCA GAGCCTTCGT GTACCTGAGT AACCTGCTGT ACCCTGTTCC TCTGGTGCAT 
CGAGTCGCTG TAGTGACAGA GAAAGGAGAT GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG 
GCCATCGCCG CGGATGAAGA GGCACCTGAT TATGGGTCTG GTGTGCGTCA GTCAGGAACT 
GCTAAGATTT CCTTTGATAA TGAGTATTTT AAAAAGAATG ACTTCACCTC AGTGGCCATG 
ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG CGCATTGTAG AAGGTCAAGG TCAGAGTTCA 
GAGGTCATTA CGCCTTCAGA GGAGATGAAC AGAATCAATG AAATGGACCT AAAGCTGGGG 
AACGTGGACA CAAAACTGGG AGACGGTTTG GCCGAGCATC TGGAAGTGGG CAGCATTTTC 
ACTTTCCGCG TCACCGTTCT GCAGGCAAGC GGCATCCCGG CCGAATACGC AGACATCTTC 
TGCCAGTTCA ATTTCCTCCA CCGTCACGAT GAGGCGTTCA GCACTGAGCC TCTGAAGAAC 
ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC CACGTACAGA ATATCTCAGT GGAGGTGACA 
GAGTCCTTCA TTGAGTACAT AAAGACCAAG CCAATTGTAT TTGAAGTGTT TGGCCATTAC 
CAGCAACACC CACTGCACAT TCATGGACAG GATGTGGTCA GCCCTTCTCA GCCTTCCAGA 
AAGTACTATC CTCCACCAAT GCCTCTGTCT AAACCAGATC ACGCTCGTAA GATTGAGCTT 
ATCCGTTACC TGATGAGCGG GTATGTTCAT GGTCAGGTGT TGGACACATT GTCAGAGCAT 
GCCAACGCTT TGGCCTCCAC CGCCGTGGCC AGTCTGGAGG AAGGGGCATA TGAGCTGTGC 
CACTTCCCCT TCCTCCCCAT ACCCAACTGC CCCGTCTTCA GAGTGCCCCG ACACGACGTG 
CCAGCCACCA AACTGAACAC CATCACCAAG TCCAACCTGG GTCAGTGTGT GAGCAAGTAC 
GACTTGCTGG CCTGGTTTGA GATCAGCGAA CTGGAGCCCA CTGGAGAGTA TATTCCAGCA 
GTAGTGGATC ATACAGGAGG TCTTCCCTGT CATGGCACAT ACCTGCTGCA CCAGGGCATC 
CAGCGCAGAA TAACAGTGAC TCTGATCCAT GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC 
GTCCGAGAGC TGGTGGTCGG TCGAATCAGG AATAAGCCAG AAGTGGATGA TTCAGCCGCA 
GACGCCGTCT TGTCTCTCAA CATCATCTCA GCTAAAAACC TCAAATCCTC CCACAACTCC 
AGCAGCCTTT GTATTGATAG CGATATTTCT AGGACGTTCT ACCGGTTTGA GGCCGTGTGG 
GACAGTTCTC TGCACAACTC TCTCCTACTG AACCGCGTCA CTCCATACGG AGAGAAGATC 
TACATGACCC TGTCTGCCTA TCTGGAGCTC GATCACTGCA TCCAGCCTGC TATCATCACC 
AAGGACATCT GTATGGTCTT CTACTCCAGA GATGCCAAGA TCTCGCCTCC TCGCTCGCTG 
AGAAACTTGT TTGGCAGCGG CTACTCCAAA ACCCCGGACT GTAATAAAGT GACTGGAGTC 
TATGAGCTGA GCTTGTGCAA GATAGCAGAC ACCGGAAGCC CAGGTATGCA GCGGCGCAGA 
CGGAAGGTTC TGGACACGTC AGTGGCATAT GTGCGTGGAG AGGAGAATCT GGCCGGCTGG 
AGACCCCGAG GAGACAGTCT GATCCTGGAG CACCAGTGGG AGCTGGACAA AATGGAGCAG 
CTGCATGAGG TGGAAAAGAC GCGTCACTTG CTTCTGCTGC GTGAGAAGCT GGGAGAAACA 
CCTCCACCTA AATCTCTGAG CGAGTCTCTG TCCCCCAGCC TGAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC CCAGATCTCC TCCACAACTT TCGAGAGTGC CATCACCCCC 
AGCGAAAGCA GCGGCTATGA CTCCACGGAC ATCGAGAGCT TGGTGGATCG AGAGAAGGAG 
CTGGCCACTA AGTGCCTCCG TCTCCTGACA CACACTTTCA ACAGTGAATA CAACCAAATG 
TGCAACAGCA TTAGTGACTG CAAGTTTTCA GATATCTCTC CTATGGGTCG GGATCCATCA 
GTCAGTAGTT TCAGCAGTGC CACCCTCACA CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC 
TCCAGGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295090.1
CDS	320..6052
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDSDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296815.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296815.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAGTG ACGATATTAA CGATGTTAAA 
ACGCTGCTAA GTTCACAAAC TGAAGCAATG CTCTATCGGA GAAAAGCACC CATGGGCTGT 
CTGACAGCCT CTCCATCTTC AGGCTCTCTC TGCAGCCAGG CAGGCCTACA GTCCGTCTCC 
AGCATCCAGG AGCGCATCAT GTCCACGCCA GGCGGAGAAG AGGCCATCGA GAGGCTCAAG 
GAATCAGAAA AGATCATAGC CGAGCTGAAT GAGACCTGGG AAGAGAAGCT GAGGAAGACC 
GAGGCCATCC GTATGGAGAG AGAGGCGCTG CTGGCAGAGA TGGGAGTTGC CATACGGGAG 
GACGGGGGAA CCCTGGGCGT CTTCTCTCCT AAAAAGTTTG GTCCTGATAG ACCAACGTCA 
CTGTCCATTG AGGACTTTAG TGTTTATTCT TCCAATCAGC TTTTCGTTGA AAGGCCCTGT 
GAAGTCTATA CTGACTTTAA TGGGGTGTTT TCACCCAAAA AGACGCCTCA CCTGGTGAAC 
CTGAACGAGG ATCCTTTGAT GTCAGAATGT CTGCTCTATT ATATCAAAGA TGGAATAACA 
AGGGTGGGCC AGGCAGATGC TGAAAGAAGA CAGGATATCG TTCTAAGTGG AGCTCACATT 
AAAGAGGAGC ACTGTATTTT CCGCAGTGAG AGGAATGCCA ATGGACATGT GATTGTCACG 
TTGGAGCCAT GTGAGGGATC AGAAACATAT GTAAATGGTA AACGTGTGAA TTCTGCGGTC 
CAGCTGCGCT CAGGTAACCG CATTATCATG GGAAAGAACC ACGTGTTCCG GTTTAACCAC 
CCTGAGCAGG CTCGCGCTGA GCGTGAGAAG ACCCCGTCAG CAGAGACACC TGTAGAACCT 
GTTGACTGGA CTTTTGCCCA AAGAGAACTG CTGGAGAAAC AGGGTATCGA CATGAAGCAA 
GAGATGGAGA AGAGGCTTAC AGAAATGGAG ATCCTGTACA AAAAAGAGAA GGAGGAAGCG 
GACCAGCTAC TGGAGCAACA GAGACTGGTG TATGAGAGCA AACTGCAAGA GCTGCAGAAA 
CAAGTGGAGA CTCGTTCTTT GGCTGCAGAG ACTCCAGATG AGGAGGAAGA AGAAGAGGAG 
GAGGATGAAG TGCCCTGGAC CCAGCATAAA TATGAGCTGG CTCAGTGGGC TTTCAGGAAG 
TGGAGATACC ACCAGTTCAC CTCCCTCAGA GACCAATTAT GGGGCAATGC TGTGTACCTC 
AAAGAAGCCA ATGCCATCAG TGTGGAGCTC AAGAAAAAGG TCCAGTTCCA GTTCGTCTTG 
CTCACAGACA CCCTCTATTC TCCACTGCCC CCGGAGCTGC TGCCTCCAGA GCCAGAGAAA 
GAGCGAGACG CCAGGCCTTT TCCGAGAACA GTGGTGGCTG TGGAGGTTCA GGACCTGAAG 
AATGGAGCCA CTCACTACTG GTCCCTGGAG AAGCTGAAGC AGAGGCTGGA CCAGATGCGA 
GAGATGTACG ACCGTGCTGG CGAAATGGCC TCGTCCAATC AGGGAGATGA TGGGGAGGGT 
GCGTTGACCG GCAGTGACCC GTTCTATGAC CGATTTCATT GGTTTAAGCT GGTTGGGAGC 
TCACCTATTT TTCATGGCTG TGTGAACGAG CGTCTGGCCG ACCGCACACC ATCGCCCACC 
TTCTCCACCA CCGATTCGGA GATCACTGAG CTGGCCGACG AGCGACAGAG CGAGATGGAG 
GACTTTATGG ATGACGAGGC TTTCGTGGAT GACACCAGCT CAGATGCTGG AACCGAGGAG 
GGCTCGGACA TCTTCAGTGA TGGCCAGGAC CCCTTCTATG ATCGTTCTCC ATGGTTCATC 
CTGGTGGGCA GAGCCTTCGT GTACCTGAGT AACCTGCTGT ACCCTGTTCC TCTGGTGCAT 
CGAGTCGCTG TAGTGACAGA GAAAGGAGAT GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG 
GCCATCGCCG CGGATGAAGA GGCACCTGAT TATGGGTCTG GTGTGCGTCA GTCAGGAACT 
GCTAAGATTT CCTTTGATAA TGAGTATTTT AAAAAGAATG ACTTCACCTC AGTGGCCATG 
ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG CGCATTGTAG AAGGTCAAGG TCAGAGTTCA 
GAGGTCATTA CGCCTTCAGA GGAGATGAAC AGAATCAATG AAATGGACCT AAAGCTGGGG 
AACGTGGACA CAAAACTGGG AGACGGTTTG GCCGAGCATC TGGAAGTGGG CAGCATTTTC 
ACTTTCCGCG TCACCGTTCT GCAGGCAAGC GGCATCCCGG CCGAATACGC AGACATCTTC 
TGCCAGTTCA ATTTCCTCCA CCGTCACGAT GAGGCGTTCA GCACTGAGCC TCTGAAGAAC 
ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC CACGTACAGA ATATCTCAGT GGAGGTGACA 
GAGTCCTTCA TTGAGTACAT AAAGACCAAG CCAATTGTAT TTGAAGTGTT TGGCCATTAC 
CAGCAACACC CACTGCACAT TCATGGACAG GATGTGGTCA GCCCTTCTCA GCCTTCCAGA 
AAGTACTATC CTCCACCAAT GCCTCTGTCT AAACCAGATC ACGCTCGTAA GATTGAGCTT 
ATCCGTTACC TGATGAGCGG GTATGTTCAT GGTCAGGTGT TGGACACATT GTCAGAGCAT 
GCCAACGCTT TGGCCTCCAC CGCCGTGGCC AGTCTGGAGG AAGGGGCATA TGAGCTGTGC 
CACTTCCCCT TCCTCCCCAT ACCCAACTGC CCCGTCTTCA GAGTGCCCCG ACACGACGTG 
CCAGCCACCA AACTGAACAC CATCACCAAG TCCAACCTGG GTCAGTGTGT GAGCAAGTAC 
GACTTGCTGG CCTGGTTTGA GATCAGCGAA CTGGAGCCCA CTGGAGAGTA TATTCCAGCA 
GTAGTGGATC ATACAGGAGG TCTTCCCTGT CATGGCACAT ACCTGCTGCA CCAGGGCATC 
CAGCGCAGAA TAACAGTGAC TCTGATCCAT GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC 
GTCCGAGAGC TGGTGGTCGG TCGAATCAGG AATAAGCCAG AAGTGGATGA TTCAGCCGCA 
GACGCCGTCT TGTCTCTCAA CATCATCTCA GCTAAAAACC TCAAATCCTC CCACAACTCC 
AGCAGCCTTT GTATTGATAG CGATATTTCT AGGACGTTCT ACCGGTTTGA GGCCGTGTGG 
GACAGTTCTC TGCACAACTC TCTCCTACTG AACCGCGTCA CTCCATACGG AGAGAAGATC 
TACATGACCC TGTCTGCCTA TCTGGAGCTC GATCACTGCA TCCAGCCTGC TATCATCACC 
AAGGACATCT GTATGGTCTT CTACTCCAGA GATGCCAAGA TCTCGCCTCC TCGCTCGCTG 
AGAAACTTGT TTGGCAGCGG CTACTCCAAA ACCCCGGACT GTAATAAAGT GACTGGAGTC 
TATGAGCTGA GCTTGTGCAA GATAGCAGAC ACCGGAAGCC CAGGTATGCA GCGGCGCAGA 
CGGAAGGTTC TGGACACGTC AGTGGCATAT GTGCGTGGAG AGGAGAATCT GGCCGGCTGG 
AGACCCCGAG GAGACAGTCT GATCCTGGAG CACCAGTGGG AGCTGGACAA AATGGAGCAG 
CTGCATGAGG TGGAAAAGAC GCGTCACTTG CTTCTGCTGC GTGAGAAGCT GGGAGAAACA 
CCTCCACCTA AATCTCTGAG CGAGTCTCTG TCCCCCAGCC TGAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC CCAGATCTCC TCCACAACTT TCGAGAGTGC CATCACCCCC 
AGCGAAAGCA GCGGCTATGA CTCCACGGAC ATCGAGAGCT TGGTGGATCG AGAGAAGGAG 
CTGGCCACTA AGTGCCTCCG TCTCCTGACA CACACTTTCA ACAGTGAATA CAACCAAATG 
TGCAACAGCA TTAGTGACTG CAAGTTTTCA GATATCTCTC CTATGGGTCG GGATCCATCA 
GTCAGTAGTT TCAGCAGTGC CACCCTCACA CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC 
TCCAGGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296815.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295090.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.

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CloneID	ODa40264
Clone ID Related Accession (Same CDS sequence)	XM_009296815.3 , XM_009296815.2
Accession Version	XM_009296815.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAGTG ACGATATTAA CGATGTTAAA 
ACGCTGCTAA GTTCACAAAC TGAAGCAATG CTCTATCGGA GAAAAGCACC CATGGGCTGT 
CTGACAGCCT CTCCATCTTC AGGCTCTCTC TGCAGCCAGG CAGGCCTACA GTCCGTCTCC 
AGCATCCAGG AGCGCATCAT GTCCACGCCA GGCGGAGAAG AGGCCATCGA GAGGCTCAAG 
GAATCAGAAA AGATCATAGC CGAGCTGAAT GAGACCTGGG AAGAGAAGCT GAGGAAGACC 
GAGGCCATCC GTATGGAGAG AGAGGCGCTG CTGGCAGAGA TGGGAGTTGC CATACGGGAG 
GACGGGGGAA CCCTGGGCGT CTTCTCTCCT AAAAAGTTTG GTCCTGATAG ACCAACGTCA 
CTGTCCATTG AGGACTTTAG TGTTTATTCT TCCAATCAGC TTTTCGTTGA AAGGCCCTGT 
GAAGTCTATA CTGACTTTAA TGGGGTGTTT TCACCCAAAA AGACGCCTCA CCTGGTGAAC 
CTGAACGAGG ATCCTTTGAT GTCAGAATGT CTGCTCTATT ATATCAAAGA TGGAATAACA 
AGGGTGGGCC AGGCAGATGC TGAAAGAAGA CAGGATATCG TTCTAAGTGG AGCTCACATT 
AAAGAGGAGC ACTGTATTTT CCGCAGTGAG AGGAATGCCA ATGGACATGT GATTGTCACG 
TTGGAGCCAT GTGAGGGATC AGAAACATAT GTAAATGGTA AACGTGTGAA TTCTGCGGTC 
CAGCTGCGCT CAGGTAACCG CATTATCATG GGAAAGAACC ACGTGTTCCG GTTTAACCAC 
CCTGAGCAGG CTCGCGCTGA GCGTGAGAAG ACCCCGTCAG CAGAGACACC TGTAGAACCT 
GTTGACTGGA CTTTTGCCCA AAGAGAACTG CTGGAGAAAC AGGGTATCGA CATGAAGCAA 
GAGATGGAGA AGAGGCTTAC AGAAATGGAG ATCCTGTACA AAAAAGAGAA GGAGGAAGCG 
GACCAGCTAC TGGAGCAACA GAGACTGGTG TATGAGAGCA AACTGCAAGA GCTGCAGAAA 
CAAGTGGAGA CTCGTTCTTT GGCTGCAGAG ACTCCAGATG AGGAGGAAGA AGAAGAGGAG 
GAGGATGAAG TGCCCTGGAC CCAGCATAAA TATGAGCTGG CTCAGTGGGC TTTCAGGAAG 
TGGAGATACC ACCAGTTCAC CTCCCTCAGA GACCAATTAT GGGGCAATGC TGTGTACCTC 
AAAGAAGCCA ATGCCATCAG TGTGGAGCTC AAGAAAAAGG TCCAGTTCCA GTTCGTCTTG 
CTCACAGACA CCCTCTATTC TCCACTGCCC CCGGAGCTGC TGCCTCCAGA GCCAGAGAAA 
GAGCGAGACG CCAGGCCTTT TCCGAGAACA GTGGTGGCTG TGGAGGTTCA GGACCTGAAG 
AATGGAGCCA CTCACTACTG GTCCCTGGAG AAGCTGAAGC AGAGGCTGGA CCAGATGCGA 
GAGATGTACG ACCGTGCTGG CGAAATGGCC TCGTCCAATC AGGGAGATGA TGGGGAGGGT 
GCGTTGACCG GCAGTGACCC GTTCTATGAC CGATTTCATT GGTTTAAGCT GGTTGGGAGC 
TCACCTATTT TTCATGGCTG TGTGAACGAG CGTCTGGCCG ACCGCACACC ATCGCCCACC 
TTCTCCACCA CCGATTCGGA GATCACTGAG CTGGCCGACG AGCGACAGAG CGAGATGGAG 
GACTTTATGG ATGACGAGGC TTTCGTGGAT GACACCAGCT CAGATGCTGG AACCGAGGAG 
GGCTCGGACA TCTTCAGTGA TGGCCAGGAC CCCTTCTATG ATCGTTCTCC ATGGTTCATC 
CTGGTGGGCA GAGCCTTCGT GTACCTGAGT AACCTGCTGT ACCCTGTTCC TCTGGTGCAT 
CGAGTCGCTG TAGTGACAGA GAAAGGAGAT GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG 
GCCATCGCCG CGGATGAAGA GGCACCTGAT TATGGGTCTG GTGTGCGTCA GTCAGGAACT 
GCTAAGATTT CCTTTGATAA TGAGTATTTT AAAAAGAATG ACTTCACCTC AGTGGCCATG 
ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG CGCATTGTAG AAGGTCAAGG TCAGAGTTCA 
GAGGTCATTA CGCCTTCAGA GGAGATGAAC AGAATCAATG AAATGGACCT AAAGCTGGGG 
AACGTGGACA CAAAACTGGG AGACGGTTTG GCCGAGCATC TGGAAGTGGG CAGCATTTTC 
ACTTTCCGCG TCACCGTTCT GCAGGCAAGC GGCATCCCGG CCGAATACGC AGACATCTTC 
TGCCAGTTCA ATTTCCTCCA CCGTCACGAT GAGGCGTTCA GCACTGAGCC TCTGAAGAAC 
ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC CACGTACAGA ATATCTCAGT GGAGGTGACA 
GAGTCCTTCA TTGAGTACAT AAAGACCAAG CCAATTGTAT TTGAAGTGTT TGGCCATTAC 
CAGCAACACC CACTGCACAT TCATGGACAG GATGTGGTCA GCCCTTCTCA GCCTTCCAGA 
AAGTACTATC CTCCACCAAT GCCTCTGTCT AAACCAGATC ACGCTCGTAA GATTGAGCTT 
ATCCGTTACC TGATGAGCGG GTATGTTCAT GGTCAGGTGT TGGACACATT GTCAGAGCAT 
GCCAACGCTT TGGCCTCCAC CGCCGTGGCC AGTCTGGAGG AAGGGGCATA TGAGCTGTGC 
CACTTCCCCT TCCTCCCCAT ACCCAACTGC CCCGTCTTCA GAGTGCCCCG ACACGACGTG 
CCAGCCACCA AACTGAACAC CATCACCAAG TCCAACCTGG GTCAGTGTGT GAGCAAGTAC 
GACTTGCTGG CCTGGTTTGA GATCAGCGAA CTGGAGCCCA CTGGAGAGTA TATTCCAGCA 
GTAGTGGATC ATACAGGAGG TCTTCCCTGT CATGGCACAT ACCTGCTGCA CCAGGGCATC 
CAGCGCAGAA TAACAGTGAC TCTGATCCAT GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC 
GTCCGAGAGC TGGTGGTCGG TCGAATCAGG AATAAGCCAG AAGTGGATGA TTCAGCCGCA 
GACGCCGTCT TGTCTCTCAA CATCATCTCA GCTAAAAACC TCAAATCCTC CCACAACTCC 
AGCAGCCTTT GTATTGATAG CGATATTTCT AGGACGTTCT ACCGGTTTGA GGCCGTGTGG 
GACAGTTCTC TGCACAACTC TCTCCTACTG AACCGCGTCA CTCCATACGG AGAGAAGATC 
TACATGACCC TGTCTGCCTA TCTGGAGCTC GATCACTGCA TCCAGCCTGC TATCATCACC 
AAGGACATCT GTATGGTCTT CTACTCCAGA GATGCCAAGA TCTCGCCTCC TCGCTCGCTG 
AGAAACTTGT TTGGCAGCGG CTACTCCAAA ACCCCGGACT GTAATAAAGT GACTGGAGTC 
TATGAGCTGA GCTTGTGCAA GATAGCAGAC ACCGGAAGCC CAGGTATGCA GCGGCGCAGA 
CGGAAGGTTC TGGACACGTC AGTGGCATAT GTGCGTGGAG AGGAGAATCT GGCCGGCTGG 
AGACCCCGAG GAGACAGTCT GATCCTGGAG CACCAGTGGG AGCTGGACAA AATGGAGCAG 
CTGCATGAGG TGGAAAAGAC GCGTCACTTG CTTCTGCTGC GTGAGAAGCT GGGAGAAACA 
CCTCCACCTA AATCTCTGAG CGAGTCTCTG TCCCCCAGCC TGAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC CCAGATCTCC TCCACAACTT TCGAGAGTGC CATCACCCCC 
AGCGAAAGCA GCGGCTATGA CTCCACGGAC ATCGAGAGCT TGGTGGATCG AGAGAAGGAG 
CTGGCCACTA AGTGCCTCCG TCTCCTGACA CACACTTTCA ACAGTGAATA CAACCAAATG 
TGCAACAGCA TTAGTGACTG CAAGTTTTCA GATATCTCTC CTATGGGTCG GGATCCATCA 
GTCAGTAGTT TCAGCAGTGC CACCCTCACA CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC 
TCCAGGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295090.1
CDS	319..6051
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDSDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296815.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296815.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACAGTG ACGATATTAA CGATGTTAAA 
ACGCTGCTAA GTTCACAAAC TGAAGCAATG CTCTATCGGA GAAAAGCACC CATGGGCTGT 
CTGACAGCCT CTCCATCTTC AGGCTCTCTC TGCAGCCAGG CAGGCCTACA GTCCGTCTCC 
AGCATCCAGG AGCGCATCAT GTCCACGCCA GGCGGAGAAG AGGCCATCGA GAGGCTCAAG 
GAATCAGAAA AGATCATAGC CGAGCTGAAT GAGACCTGGG AAGAGAAGCT GAGGAAGACC 
GAGGCCATCC GTATGGAGAG AGAGGCGCTG CTGGCAGAGA TGGGAGTTGC CATACGGGAG 
GACGGGGGAA CCCTGGGCGT CTTCTCTCCT AAAAAGTTTG GTCCTGATAG ACCAACGTCA 
CTGTCCATTG AGGACTTTAG TGTTTATTCT TCCAATCAGC TTTTCGTTGA AAGGCCCTGT 
GAAGTCTATA CTGACTTTAA TGGGGTGTTT TCACCCAAAA AGACGCCTCA CCTGGTGAAC 
CTGAACGAGG ATCCTTTGAT GTCAGAATGT CTGCTCTATT ATATCAAAGA TGGAATAACA 
AGGGTGGGCC AGGCAGATGC TGAAAGAAGA CAGGATATCG TTCTAAGTGG AGCTCACATT 
AAAGAGGAGC ACTGTATTTT CCGCAGTGAG AGGAATGCCA ATGGACATGT GATTGTCACG 
TTGGAGCCAT GTGAGGGATC AGAAACATAT GTAAATGGTA AACGTGTGAA TTCTGCGGTC 
CAGCTGCGCT CAGGTAACCG CATTATCATG GGAAAGAACC ACGTGTTCCG GTTTAACCAC 
CCTGAGCAGG CTCGCGCTGA GCGTGAGAAG ACCCCGTCAG CAGAGACACC TGTAGAACCT 
GTTGACTGGA CTTTTGCCCA AAGAGAACTG CTGGAGAAAC AGGGTATCGA CATGAAGCAA 
GAGATGGAGA AGAGGCTTAC AGAAATGGAG ATCCTGTACA AAAAAGAGAA GGAGGAAGCG 
GACCAGCTAC TGGAGCAACA GAGACTGGTG TATGAGAGCA AACTGCAAGA GCTGCAGAAA 
CAAGTGGAGA CTCGTTCTTT GGCTGCAGAG ACTCCAGATG AGGAGGAAGA AGAAGAGGAG 
GAGGATGAAG TGCCCTGGAC CCAGCATAAA TATGAGCTGG CTCAGTGGGC TTTCAGGAAG 
TGGAGATACC ACCAGTTCAC CTCCCTCAGA GACCAATTAT GGGGCAATGC TGTGTACCTC 
AAAGAAGCCA ATGCCATCAG TGTGGAGCTC AAGAAAAAGG TCCAGTTCCA GTTCGTCTTG 
CTCACAGACA CCCTCTATTC TCCACTGCCC CCGGAGCTGC TGCCTCCAGA GCCAGAGAAA 
GAGCGAGACG CCAGGCCTTT TCCGAGAACA GTGGTGGCTG TGGAGGTTCA GGACCTGAAG 
AATGGAGCCA CTCACTACTG GTCCCTGGAG AAGCTGAAGC AGAGGCTGGA CCAGATGCGA 
GAGATGTACG ACCGTGCTGG CGAAATGGCC TCGTCCAATC AGGGAGATGA TGGGGAGGGT 
GCGTTGACCG GCAGTGACCC GTTCTATGAC CGATTTCATT GGTTTAAGCT GGTTGGGAGC 
TCACCTATTT TTCATGGCTG TGTGAACGAG CGTCTGGCCG ACCGCACACC ATCGCCCACC 
TTCTCCACCA CCGATTCGGA GATCACTGAG CTGGCCGACG AGCGACAGAG CGAGATGGAG 
GACTTTATGG ATGACGAGGC TTTCGTGGAT GACACCAGCT CAGATGCTGG AACCGAGGAG 
GGCTCGGACA TCTTCAGTGA TGGCCAGGAC CCCTTCTATG ATCGTTCTCC ATGGTTCATC 
CTGGTGGGCA GAGCCTTCGT GTACCTGAGT AACCTGCTGT ACCCTGTTCC TCTGGTGCAT 
CGAGTCGCTG TAGTGACAGA GAAAGGAGAT GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG 
GCCATCGCCG CGGATGAAGA GGCACCTGAT TATGGGTCTG GTGTGCGTCA GTCAGGAACT 
GCTAAGATTT CCTTTGATAA TGAGTATTTT AAAAAGAATG ACTTCACCTC AGTGGCCATG 
ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG CGCATTGTAG AAGGTCAAGG TCAGAGTTCA 
GAGGTCATTA CGCCTTCAGA GGAGATGAAC AGAATCAATG AAATGGACCT AAAGCTGGGG 
AACGTGGACA CAAAACTGGG AGACGGTTTG GCCGAGCATC TGGAAGTGGG CAGCATTTTC 
ACTTTCCGCG TCACCGTTCT GCAGGCAAGC GGCATCCCGG CCGAATACGC AGACATCTTC 
TGCCAGTTCA ATTTCCTCCA CCGTCACGAT GAGGCGTTCA GCACTGAGCC TCTGAAGAAC 
ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC CACGTACAGA ATATCTCAGT GGAGGTGACA 
GAGTCCTTCA TTGAGTACAT AAAGACCAAG CCAATTGTAT TTGAAGTGTT TGGCCATTAC 
CAGCAACACC CACTGCACAT TCATGGACAG GATGTGGTCA GCCCTTCTCA GCCTTCCAGA 
AAGTACTATC CTCCACCAAT GCCTCTGTCT AAACCAGATC ACGCTCGTAA GATTGAGCTT 
ATCCGTTACC TGATGAGCGG GTATGTTCAT GGTCAGGTGT TGGACACATT GTCAGAGCAT 
GCCAACGCTT TGGCCTCCAC CGCCGTGGCC AGTCTGGAGG AAGGGGCATA TGAGCTGTGC 
CACTTCCCCT TCCTCCCCAT ACCCAACTGC CCCGTCTTCA GAGTGCCCCG ACACGACGTG 
CCAGCCACCA AACTGAACAC CATCACCAAG TCCAACCTGG GTCAGTGTGT GAGCAAGTAC 
GACTTGCTGG CCTGGTTTGA GATCAGCGAA CTGGAGCCCA CTGGAGAGTA TATTCCAGCA 
GTAGTGGATC ATACAGGAGG TCTTCCCTGT CATGGCACAT ACCTGCTGCA CCAGGGCATC 
CAGCGCAGAA TAACAGTGAC TCTGATCCAT GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC 
GTCCGAGAGC TGGTGGTCGG TCGAATCAGG AATAAGCCAG AAGTGGATGA TTCAGCCGCA 
GACGCCGTCT TGTCTCTCAA CATCATCTCA GCTAAAAACC TCAAATCCTC CCACAACTCC 
AGCAGCCTTT GTATTGATAG CGATATTTCT AGGACGTTCT ACCGGTTTGA GGCCGTGTGG 
GACAGTTCTC TGCACAACTC TCTCCTACTG AACCGCGTCA CTCCATACGG AGAGAAGATC 
TACATGACCC TGTCTGCCTA TCTGGAGCTC GATCACTGCA TCCAGCCTGC TATCATCACC 
AAGGACATCT GTATGGTCTT CTACTCCAGA GATGCCAAGA TCTCGCCTCC TCGCTCGCTG 
AGAAACTTGT TTGGCAGCGG CTACTCCAAA ACCCCGGACT GTAATAAAGT GACTGGAGTC 
TATGAGCTGA GCTTGTGCAA GATAGCAGAC ACCGGAAGCC CAGGTATGCA GCGGCGCAGA 
CGGAAGGTTC TGGACACGTC AGTGGCATAT GTGCGTGGAG AGGAGAATCT GGCCGGCTGG 
AGACCCCGAG GAGACAGTCT GATCCTGGAG CACCAGTGGG AGCTGGACAA AATGGAGCAG 
CTGCATGAGG TGGAAAAGAC GCGTCACTTG CTTCTGCTGC GTGAGAAGCT GGGAGAAACA 
CCTCCACCTA AATCTCTGAG CGAGTCTCTG TCCCCCAGCC TGAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC CCAGATCTCC TCCACAACTT TCGAGAGTGC CATCACCCCC 
AGCGAAAGCA GCGGCTATGA CTCCACGGAC ATCGAGAGCT TGGTGGATCG AGAGAAGGAG 
CTGGCCACTA AGTGCCTCCG TCTCCTGACA CACACTTTCA ACAGTGAATA CAACCAAATG 
TGCAACAGCA TTAGTGACTG CAAGTTTTCA GATATCTCTC CTATGGGTCG GGATCCATCA 
GTCAGTAGTT TCAGCAGTGC CACCCTCACA CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC 
TCCAGGACCC CTGAAGCAAA CTCTCGTGCA ACCAGCCCCT CAGGCTCAGA CTATGAGAAC 
TTCCCCATGG TGCCCATTCT GGAGACGTCC TACCTGGCCC GCGCCGGCAA ACACGAGTTC 
CTCAACCTTG TGCCTAACAT AGAGGAAATG AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC 
ATCTTGAATT TCATGGAGCC CCGCTCGAAC ACCTGGGTGA AGCACTTTGT GGTGGTGCGT 
CGGCCGTATG TGTTCATCTA CAACAATGAC AAGGACCCTG TGGAGAGAGG AGTGCTGAAC 
TTATCCACTG CACAGGTGGA GTACAGTGAA GACCAGCAGG CCATGCTCAA GACGCCTCAT 
ACATTTGCCA TGTGCACCAA ACACAGAGGA ATCCTGCTTC AGGCCAACAA TGACAAAGAC 
ATGAACGACT GGCTGTATGC CTTTAACCCA CTGCTAGCAG GAACAATAAG GTCGAAGCTT 
GCAAGGAGAC GGTCTGGTGT GATGAAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296816.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295091.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.

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CloneID	ODa40265
Clone ID Related Accession (Same CDS sequence)	XM_009296816.3 , XM_009296816.2
Accession Version	XM_009296816.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGT TTGGTCCTGA TAGACCAACG TCACTGTCCA TTGAGGACTT TAGTGTTTAT 
TCTTCCAATC AGCTTTTCGT TGAAAGGCCC TGTGAAGTCT ATACTGACTT TAATGGGGTG 
TTTTCACCCA AAAAGACGCC TCACCTGGTG AACCTGAACG AGGATCCTTT GATGTCAGAA 
TGTCTGCTCT ATTATATCAA AGATGGAATA ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA 
AGACAGGATA TCGTTCTAAG TGGAGCTCAC ATTAAAGAGG AGCACTGTAT TTTCCGCAGT 
GAGAGGAATG CCAATGGACA TGTGATTGTC ACGTTGGAGC CATGTGAGGG ATCAGAAACA 
TATGTAAATG GTAAACGTGT GAATTCTGCG GTCCAGCTGC GCTCAGGTAA CCGCATTATC 
ATGGGAAAGA ACCACGTGTT CCGGTTTAAC CACCCTGAGC AGGCTCGCGC TGAGCGTGAG 
AAGACCCCGT CAGCAGAGAC ACCTGTAGAA CCTGTTGACT GGACTTTTGC CCAAAGAGAA 
CTGCTGGAGA AACAGGGTAT CGACATGAAG CAAGAGATGG AGAAGAGGCT TACAGAAATG 
GAGATCCTGT ACAAAAAAGA GAAGGAGGAA GCGGACCAGC TACTGGAGCA ACAGAGACTG 
GTGTATGAGA GCAAACTGCA AGAGCTGCAG AAACAAGTGG AGACTCGTTC TTTGGCTGCA 
GAGACTCCAG ATGAGGAGGA AGAAGAAGAG GAGGAGGATG AAGTGCCCTG GACCCAGCAT 
AAATATGAGC TGGCTCAGTG GGCTTTCAGG AAGTGGAGAT ACCACCAGTT CACCTCCCTC 
AGAGACCAAT TATGGGGCAA TGCTGTGTAC CTCAAAGAAG CCAATGCCAT CAGTGTGGAG 
CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC TTGCTCACAG ACACCCTCTA TTCTCCACTG 
CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA 
ACAGTGGTGG CTGTGGAGGT TCAGGACCTG AAGAATGGAG CCACTCACTA CTGGTCCCTG 
GAGAAGCTGA AGCAGAGGCT GGACCAGATG CGAGAGATGT ACGACCGTGC TGGCGAAATG 
GCCTCGTCCA ATCAGGGAGA TGATGGGGAG GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT 
GACCGATTTC ATTGGTTTAA GCTGGTTGGG AGCTCACCTA TTTTTCATGG CTGTGTGAAC 
GAGCGTCTGG CCGACCGCAC ACCATCGCCC ACCTTCTCCA CCACCGATTC GGAGATCACT 
GAGCTGGCCG ACGAGCGACA GAGCGAGATG GAGGACTTTA TGGATGACGA GGCTTTCGTG 
GATGACACCA GCTCAGATGC TGGAACCGAG GAGGGCTCGG ACATCTTCAG TGATGGCCAG 
GACCCCTTCT ATGATCGTTC TCCATGGTTC ATCCTGGTGG GCAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295091.1
CDS	320..6025
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296816.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296816.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGT TTGGTCCTGA TAGACCAACG TCACTGTCCA TTGAGGACTT TAGTGTTTAT 
TCTTCCAATC AGCTTTTCGT TGAAAGGCCC TGTGAAGTCT ATACTGACTT TAATGGGGTG 
TTTTCACCCA AAAAGACGCC TCACCTGGTG AACCTGAACG AGGATCCTTT GATGTCAGAA 
TGTCTGCTCT ATTATATCAA AGATGGAATA ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA 
AGACAGGATA TCGTTCTAAG TGGAGCTCAC ATTAAAGAGG AGCACTGTAT TTTCCGCAGT 
GAGAGGAATG CCAATGGACA TGTGATTGTC ACGTTGGAGC CATGTGAGGG ATCAGAAACA 
TATGTAAATG GTAAACGTGT GAATTCTGCG GTCCAGCTGC GCTCAGGTAA CCGCATTATC 
ATGGGAAAGA ACCACGTGTT CCGGTTTAAC CACCCTGAGC AGGCTCGCGC TGAGCGTGAG 
AAGACCCCGT CAGCAGAGAC ACCTGTAGAA CCTGTTGACT GGACTTTTGC CCAAAGAGAA 
CTGCTGGAGA AACAGGGTAT CGACATGAAG CAAGAGATGG AGAAGAGGCT TACAGAAATG 
GAGATCCTGT ACAAAAAAGA GAAGGAGGAA GCGGACCAGC TACTGGAGCA ACAGAGACTG 
GTGTATGAGA GCAAACTGCA AGAGCTGCAG AAACAAGTGG AGACTCGTTC TTTGGCTGCA 
GAGACTCCAG ATGAGGAGGA AGAAGAAGAG GAGGAGGATG AAGTGCCCTG GACCCAGCAT 
AAATATGAGC TGGCTCAGTG GGCTTTCAGG AAGTGGAGAT ACCACCAGTT CACCTCCCTC 
AGAGACCAAT TATGGGGCAA TGCTGTGTAC CTCAAAGAAG CCAATGCCAT CAGTGTGGAG 
CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC TTGCTCACAG ACACCCTCTA TTCTCCACTG 
CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA 
ACAGTGGTGG CTGTGGAGGT TCAGGACCTG AAGAATGGAG CCACTCACTA CTGGTCCCTG 
GAGAAGCTGA AGCAGAGGCT GGACCAGATG CGAGAGATGT ACGACCGTGC TGGCGAAATG 
GCCTCGTCCA ATCAGGGAGA TGATGGGGAG GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT 
GACCGATTTC ATTGGTTTAA GCTGGTTGGG AGCTCACCTA TTTTTCATGG CTGTGTGAAC 
GAGCGTCTGG CCGACCGCAC ACCATCGCCC ACCTTCTCCA CCACCGATTC GGAGATCACT 
GAGCTGGCCG ACGAGCGACA GAGCGAGATG GAGGACTTTA TGGATGACGA GGCTTTCGTG 
GATGACACCA GCTCAGATGC TGGAACCGAG GAGGGCTCGG ACATCTTCAG TGATGGCCAG 
GACCCCTTCT ATGATCGTTC TCCATGGTTC ATCCTGGTGG GCAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296816.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295091.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.

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CloneID	ODa40265
Clone ID Related Accession (Same CDS sequence)	XM_009296816.3 , XM_009296816.2
Accession Version	XM_009296816.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGT TTGGTCCTGA TAGACCAACG TCACTGTCCA TTGAGGACTT TAGTGTTTAT 
TCTTCCAATC AGCTTTTCGT TGAAAGGCCC TGTGAAGTCT ATACTGACTT TAATGGGGTG 
TTTTCACCCA AAAAGACGCC TCACCTGGTG AACCTGAACG AGGATCCTTT GATGTCAGAA 
TGTCTGCTCT ATTATATCAA AGATGGAATA ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA 
AGACAGGATA TCGTTCTAAG TGGAGCTCAC ATTAAAGAGG AGCACTGTAT TTTCCGCAGT 
GAGAGGAATG CCAATGGACA TGTGATTGTC ACGTTGGAGC CATGTGAGGG ATCAGAAACA 
TATGTAAATG GTAAACGTGT GAATTCTGCG GTCCAGCTGC GCTCAGGTAA CCGCATTATC 
ATGGGAAAGA ACCACGTGTT CCGGTTTAAC CACCCTGAGC AGGCTCGCGC TGAGCGTGAG 
AAGACCCCGT CAGCAGAGAC ACCTGTAGAA CCTGTTGACT GGACTTTTGC CCAAAGAGAA 
CTGCTGGAGA AACAGGGTAT CGACATGAAG CAAGAGATGG AGAAGAGGCT TACAGAAATG 
GAGATCCTGT ACAAAAAAGA GAAGGAGGAA GCGGACCAGC TACTGGAGCA ACAGAGACTG 
GTGTATGAGA GCAAACTGCA AGAGCTGCAG AAACAAGTGG AGACTCGTTC TTTGGCTGCA 
GAGACTCCAG ATGAGGAGGA AGAAGAAGAG GAGGAGGATG AAGTGCCCTG GACCCAGCAT 
AAATATGAGC TGGCTCAGTG GGCTTTCAGG AAGTGGAGAT ACCACCAGTT CACCTCCCTC 
AGAGACCAAT TATGGGGCAA TGCTGTGTAC CTCAAAGAAG CCAATGCCAT CAGTGTGGAG 
CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC TTGCTCACAG ACACCCTCTA TTCTCCACTG 
CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA 
ACAGTGGTGG CTGTGGAGGT TCAGGACCTG AAGAATGGAG CCACTCACTA CTGGTCCCTG 
GAGAAGCTGA AGCAGAGGCT GGACCAGATG CGAGAGATGT ACGACCGTGC TGGCGAAATG 
GCCTCGTCCA ATCAGGGAGA TGATGGGGAG GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT 
GACCGATTTC ATTGGTTTAA GCTGGTTGGG AGCTCACCTA TTTTTCATGG CTGTGTGAAC 
GAGCGTCTGG CCGACCGCAC ACCATCGCCC ACCTTCTCCA CCACCGATTC GGAGATCACT 
GAGCTGGCCG ACGAGCGACA GAGCGAGATG GAGGACTTTA TGGATGACGA GGCTTTCGTG 
GATGACACCA GCTCAGATGC TGGAACCGAG GAGGGCTCGG ACATCTTCAG TGATGGCCAG 
GACCCCTTCT ATGATCGTTC TCCATGGTTC ATCCTGGTGG GCAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295091.1
CDS	319..6024
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296816.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296816.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGT TTGGTCCTGA TAGACCAACG TCACTGTCCA TTGAGGACTT TAGTGTTTAT 
TCTTCCAATC AGCTTTTCGT TGAAAGGCCC TGTGAAGTCT ATACTGACTT TAATGGGGTG 
TTTTCACCCA AAAAGACGCC TCACCTGGTG AACCTGAACG AGGATCCTTT GATGTCAGAA 
TGTCTGCTCT ATTATATCAA AGATGGAATA ACAAGGGTGG GCCAGGCAGA TGCTGAAAGA 
AGACAGGATA TCGTTCTAAG TGGAGCTCAC ATTAAAGAGG AGCACTGTAT TTTCCGCAGT 
GAGAGGAATG CCAATGGACA TGTGATTGTC ACGTTGGAGC CATGTGAGGG ATCAGAAACA 
TATGTAAATG GTAAACGTGT GAATTCTGCG GTCCAGCTGC GCTCAGGTAA CCGCATTATC 
ATGGGAAAGA ACCACGTGTT CCGGTTTAAC CACCCTGAGC AGGCTCGCGC TGAGCGTGAG 
AAGACCCCGT CAGCAGAGAC ACCTGTAGAA CCTGTTGACT GGACTTTTGC CCAAAGAGAA 
CTGCTGGAGA AACAGGGTAT CGACATGAAG CAAGAGATGG AGAAGAGGCT TACAGAAATG 
GAGATCCTGT ACAAAAAAGA GAAGGAGGAA GCGGACCAGC TACTGGAGCA ACAGAGACTG 
GTGTATGAGA GCAAACTGCA AGAGCTGCAG AAACAAGTGG AGACTCGTTC TTTGGCTGCA 
GAGACTCCAG ATGAGGAGGA AGAAGAAGAG GAGGAGGATG AAGTGCCCTG GACCCAGCAT 
AAATATGAGC TGGCTCAGTG GGCTTTCAGG AAGTGGAGAT ACCACCAGTT CACCTCCCTC 
AGAGACCAAT TATGGGGCAA TGCTGTGTAC CTCAAAGAAG CCAATGCCAT CAGTGTGGAG 
CTCAAGAAAA AGGTCCAGTT CCAGTTCGTC TTGCTCACAG ACACCCTCTA TTCTCCACTG 
CCCCCGGAGC TGCTGCCTCC AGAGCCAGAG AAAGAGCGAG ACGCCAGGCC TTTTCCGAGA 
ACAGTGGTGG CTGTGGAGGT TCAGGACCTG AAGAATGGAG CCACTCACTA CTGGTCCCTG 
GAGAAGCTGA AGCAGAGGCT GGACCAGATG CGAGAGATGT ACGACCGTGC TGGCGAAATG 
GCCTCGTCCA ATCAGGGAGA TGATGGGGAG GGTGCGTTGA CCGGCAGTGA CCCGTTCTAT 
GACCGATTTC ATTGGTTTAA GCTGGTTGGG AGCTCACCTA TTTTTCATGG CTGTGTGAAC 
GAGCGTCTGG CCGACCGCAC ACCATCGCCC ACCTTCTCCA CCACCGATTC GGAGATCACT 
GAGCTGGCCG ACGAGCGACA GAGCGAGATG GAGGACTTTA TGGATGACGA GGCTTTCGTG 
GATGACACCA GCTCAGATGC TGGAACCGAG GAGGGCTCGG ACATCTTCAG TGATGGCCAG 
GACCCCTTCT ATGATCGTTC TCCATGGTTC ATCCTGGTGG GCAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296817.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295092.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.

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CloneID	ODa40266
Clone ID Related Accession (Same CDS sequence)	XM_009296817.2 , XM_009296817.3
Accession Version	XM_009296817.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGTTT GGTCCTGATA GACCAACGTC ACTGTCCATT 
GAGGACTTTA GTGTTTATTC TTCCAATCAG CTTTTCGTTG AAAGGCCCTG TGAAGTCTAT 
ACTGACTTTA ATGGGGTGTT TTCACCCAAA AAGACGCCTC ACCTGGTGAA CCTGAACGAG 
GATCCTTTGA TGTCAGAATG TCTGCTCTAT TATATCAAAG ATGGAATAAC AAGGGTGGGC 
CAGGCAGATG CTGAAAGAAG ACAGGATATC GTTCTAAGTG GAGCTCACAT TAAAGAGGAG 
CACTGTATTT TCCGCAGTGA GAGGAATGCC AATGGACATG TGATTGTCAC GTTGGAGCCA 
TGTGAGGGAT CAGAAACATA TGTAAATGGT AAACGTGTGA ATTCTGCGGT CCAGCTGCGC 
TCAGGTAACC GCATTATCAT GGGAAAGAAC CACGTGTTCC GGTTTAACCA CCCTGAGCAG 
GCTCGCGCTG AGCGTGAGAA GACCCCGTCA GCAGAGACAC CTGTAGAACC TGTTGACTGG 
ACTTTTGCCC AAAGAGAACT GCTGGAGAAA CAGGGTATCG ACATGAAGCA AGAGATGGAG 
AAGAGGCTTA CAGAAATGGA GATCCTGTAC AAAAAAGAGA AGGAGGAAGC GGACCAGCTA 
CTGGAGCAAC AGAGACTGGT GTATGAGAGC AAACTGCAAG AGCTGCAGAA ACAAGTGGAG 
ACTCGTTCTT TGGCTGCAGA GACTCCAGAT GAGGAGGAAG AAGAAGAGGA GGAGGATGAA 
GTGCCCTGGA CCCAGCATAA ATATGAGCTG GCTCAGTGGG CTTTCAGGAA GTGGAGATAC 
CACCAGTTCA CCTCCCTCAG AGACCAATTA TGGGGCAATG CTGTGTACCT CAAAGAAGCC 
AATGCCATCA GTGTGGAGCT CAAGAAAAAG GTCCAGTTCC AGTTCGTCTT GCTCACAGAC 
ACCCTCTATT CTCCACTGCC CCCGGAGCTG CTGCCTCCAG AGCCAGAGAA AGAGCGAGAC 
GCCAGGCCTT TTCCGAGAAC AGTGGTGGCT GTGGAGGTTC AGGACCTGAA GAATGGAGCC 
ACTCACTACT GGTCCCTGGA GAAGCTGAAG CAGAGGCTGG ACCAGATGCG AGAGATGTAC 
GACCGTGCTG GCGAAATGGC CTCGTCCAAT CAGGGAGATG ATGGGGAGGG TGCGTTGACC 
GGCAGTGACC CGTTCTATGA CCGATTTCAT TGGTTTAAGC TGGTTGGGAG CTCACCTATT 
TTTCATGGCT GTGTGAACGA GCGTCTGGCC GACCGCACAC CATCGCCCAC CTTCTCCACC 
ACCGATTCGG AGATCACTGA GCTGGCCGAC GAGCGACAGA GCGAGATGGA GGACTTTATG 
GATGACGAGG CTTTCGTGGA TGACACCAGC TCAGATGCTG GAACCGAGGA GGGCTCGGAC 
ATCTTCAGTG ATGGCCAGGA CCCCTTCTAT GATCGTTCTC CATGGTTCAT CCTGGTGGGC 
AGAGCCTTCG TGTACCTGAG TAACCTGCTG TACCCTGTTC CTCTGGTGCA TCGAGTCGCT 
GTAGTGACAG AGAAAGGAGA TGTGCGAGGA TTTCTGCGTG TGGGGGTCCA GGCCATCGCC 
GCGGATGAAG AGGCACCTGA TTATGGGTCT GGTGTGCGTC AGTCAGGAAC TGCTAAGATT 
TCCTTTGATA ATGAGTATTT TAAAAAGAAT GACTTCACCT CAGTGGCCAT GACCCGCTCT 
GGTCTCTCTC TGGAGGAGCT GCGCATTGTA GAAGGTCAAG GTCAGAGTTC AGAGGTCATT 
ACGCCTTCAG AGGAGATGAA CAGAATCAAT GAAATGGACC TAAAGCTGGG GAACGTGGAC 
ACAAAACTGG GAGACGGTTT GGCCGAGCAT CTGGAAGTGG GCAGCATTTT CACTTTCCGC 
GTCACCGTTC TGCAGGCAAG CGGCATCCCG GCCGAATACG CAGACATCTT CTGCCAGTTC 
AATTTCCTCC ACCGTCACGA TGAGGCGTTC AGCACTGAGC CTCTGAAGAA CACTGGCAGA 
GGAGCCCCGC TGGGCTTTTA CCACGTACAG AATATCTCAG TGGAGGTGAC AGAGTCCTTC 
ATTGAGTACA TAAAGACCAA GCCAATTGTA TTTGAAGTGT TTGGCCATTA CCAGCAACAC 
CCACTGCACA TTCATGGACA GGATGTGGTC AGCCCTTCTC AGCCTTCCAG AAAGTACTAT 
CCTCCACCAA TGCCTCTGTC TAAACCAGAT CACGCTCGTA AGATTGAGCT TATCCGTTAC 
CTGATGAGCG GGTATGTTCA TGGTCAGGTG TTGGACACAT TGTCAGAGCA TGCCAACGCT 
TTGGCCTCCA CCGCCGTGGC CAGTCTGGAG GAAGGGGCAT ATGAGCTGTG CCACTTCCCC 
TTCCTCCCCA TACCCAACTG CCCCGTCTTC AGAGTGCCCC GACACGACGT GCCAGCCACC 
AAACTGAACA CCATCACCAA GTCCAACCTG GGTCAGTGTG TGAGCAAGTA CGACTTGCTG 
GCCTGGTTTG AGATCAGCGA ACTGGAGCCC ACTGGAGAGT ATATTCCAGC AGTAGTGGAT 
CATACAGGAG GTCTTCCCTG TCATGGCACA TACCTGCTGC ACCAGGGCAT CCAGCGCAGA 
ATAACAGTGA CTCTGATCCA TGAGAAGGGA AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG 
CTGGTGGTCG GTCGAATCAG GAATAAGCCA GAAGTGGATG ATTCAGCCGC AGACGCCGTC 
TTGTCTCTCA ACATCATCTC AGCTAAAAAC CTCAAATCCT CCCACAACTC CAGCAGCCTT 
TGTATTGATA GCGATATTTC TAGGACGTTC TACCGGTTTG AGGCCGTGTG GGACAGTTCT 
CTGCACAACT CTCTCCTACT GAACCGCGTC ACTCCATACG GAGAGAAGAT CTACATGACC 
CTGTCTGCCT ATCTGGAGCT CGATCACTGC ATCCAGCCTG CTATCATCAC CAAGGACATC 
TGTATGGTCT TCTACTCCAG AGATGCCAAG ATCTCGCCTC CTCGCTCGCT GAGAAACTTG 
TTTGGCAGCG GCTACTCCAA AACCCCGGAC TGTAATAAAG TGACTGGAGT CTATGAGCTG 
AGCTTGTGCA AGATAGCAGA CACCGGAAGC CCAGGTATGC AGCGGCGCAG ACGGAAGGTT 
CTGGACACGT CAGTGGCATA TGTGCGTGGA GAGGAGAATC TGGCCGGCTG GAGACCCCGA 
GGAGACAGTC TGATCCTGGA GCACCAGTGG GAGCTGGACA AAATGGAGCA GCTGCATGAG 
GTGGAAAAGA CGCGTCACTT GCTTCTGCTG CGTGAGAAGC TGGGAGAAAC ACCTCCACCT 
AAATCTCTGA GCGAGTCTCT GTCCCCCAGC CTGAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CCCAGATCTC CTCCACAACT TTCGAGAGTG CCATCACCCC CAGCGAAAGC 
AGCGGCTATG ACTCCACGGA CATCGAGAGC TTGGTGGATC GAGAGAAGGA GCTGGCCACT 
AAGTGCCTCC GTCTCCTGAC ACACACTTTC AACAGTGAAT ACAACCAAAT GTGCAACAGC 
ATTAGTGACT GCAAGTTTTC AGATATCTCT CCTATGGGTC GGGATCCATC AGTCAGTAGT 
TTCAGCAGTG CCACCCTCAC ACCCTCCTCC ACCTGCCCCT CGCTCTCAGA CTCCAGGACC 
CCTGAAGCAA ACTCTCGTGC AACCAGCCCC TCAGGCTCAG ACTATGAGAA CTTCCCCATG 
GTGCCCATTC TGGAGACGTC CTACCTGGCC CGCGCCGGCA AACACGAGTT CCTCAACCTT 
GTGCCTAACA TAGAGGAAAT GAGGCCTGGG TCTGTGGTGT CCAAGAAGGG CATCTTGAAT 
TTCATGGAGC CCCGCTCGAA CACCTGGGTG AAGCACTTTG TGGTGGTGCG TCGGCCGTAT 
GTGTTCATCT ACAACAATGA CAAGGACCCT GTGGAGAGAG GAGTGCTGAA CTTATCCACT 
GCACAGGTGG AGTACAGTGA AGACCAGCAG GCCATGCTCA AGACGCCTCA TACATTTGCC 
ATGTGCACCA AACACAGAGG AATCCTGCTT CAGGCCAACA ATGACAAAGA CATGAACGAC 
TGGCTGTATG CCTTTAACCC ACTGCTAGCA GGAACAATAA GGTCGAAGCT TGCAAGGAGA 
CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295092.1
CDS	319..5982
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296817.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296817.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGTTT GGTCCTGATA GACCAACGTC ACTGTCCATT 
GAGGACTTTA GTGTTTATTC TTCCAATCAG CTTTTCGTTG AAAGGCCCTG TGAAGTCTAT 
ACTGACTTTA ATGGGGTGTT TTCACCCAAA AAGACGCCTC ACCTGGTGAA CCTGAACGAG 
GATCCTTTGA TGTCAGAATG TCTGCTCTAT TATATCAAAG ATGGAATAAC AAGGGTGGGC 
CAGGCAGATG CTGAAAGAAG ACAGGATATC GTTCTAAGTG GAGCTCACAT TAAAGAGGAG 
CACTGTATTT TCCGCAGTGA GAGGAATGCC AATGGACATG TGATTGTCAC GTTGGAGCCA 
TGTGAGGGAT CAGAAACATA TGTAAATGGT AAACGTGTGA ATTCTGCGGT CCAGCTGCGC 
TCAGGTAACC GCATTATCAT GGGAAAGAAC CACGTGTTCC GGTTTAACCA CCCTGAGCAG 
GCTCGCGCTG AGCGTGAGAA GACCCCGTCA GCAGAGACAC CTGTAGAACC TGTTGACTGG 
ACTTTTGCCC AAAGAGAACT GCTGGAGAAA CAGGGTATCG ACATGAAGCA AGAGATGGAG 
AAGAGGCTTA CAGAAATGGA GATCCTGTAC AAAAAAGAGA AGGAGGAAGC GGACCAGCTA 
CTGGAGCAAC AGAGACTGGT GTATGAGAGC AAACTGCAAG AGCTGCAGAA ACAAGTGGAG 
ACTCGTTCTT TGGCTGCAGA GACTCCAGAT GAGGAGGAAG AAGAAGAGGA GGAGGATGAA 
GTGCCCTGGA CCCAGCATAA ATATGAGCTG GCTCAGTGGG CTTTCAGGAA GTGGAGATAC 
CACCAGTTCA CCTCCCTCAG AGACCAATTA TGGGGCAATG CTGTGTACCT CAAAGAAGCC 
AATGCCATCA GTGTGGAGCT CAAGAAAAAG GTCCAGTTCC AGTTCGTCTT GCTCACAGAC 
ACCCTCTATT CTCCACTGCC CCCGGAGCTG CTGCCTCCAG AGCCAGAGAA AGAGCGAGAC 
GCCAGGCCTT TTCCGAGAAC AGTGGTGGCT GTGGAGGTTC AGGACCTGAA GAATGGAGCC 
ACTCACTACT GGTCCCTGGA GAAGCTGAAG CAGAGGCTGG ACCAGATGCG AGAGATGTAC 
GACCGTGCTG GCGAAATGGC CTCGTCCAAT CAGGGAGATG ATGGGGAGGG TGCGTTGACC 
GGCAGTGACC CGTTCTATGA CCGATTTCAT TGGTTTAAGC TGGTTGGGAG CTCACCTATT 
TTTCATGGCT GTGTGAACGA GCGTCTGGCC GACCGCACAC CATCGCCCAC CTTCTCCACC 
ACCGATTCGG AGATCACTGA GCTGGCCGAC GAGCGACAGA GCGAGATGGA GGACTTTATG 
GATGACGAGG CTTTCGTGGA TGACACCAGC TCAGATGCTG GAACCGAGGA GGGCTCGGAC 
ATCTTCAGTG ATGGCCAGGA CCCCTTCTAT GATCGTTCTC CATGGTTCAT CCTGGTGGGC 
AGAGCCTTCG TGTACCTGAG TAACCTGCTG TACCCTGTTC CTCTGGTGCA TCGAGTCGCT 
GTAGTGACAG AGAAAGGAGA TGTGCGAGGA TTTCTGCGTG TGGGGGTCCA GGCCATCGCC 
GCGGATGAAG AGGCACCTGA TTATGGGTCT GGTGTGCGTC AGTCAGGAAC TGCTAAGATT 
TCCTTTGATA ATGAGTATTT TAAAAAGAAT GACTTCACCT CAGTGGCCAT GACCCGCTCT 
GGTCTCTCTC TGGAGGAGCT GCGCATTGTA GAAGGTCAAG GTCAGAGTTC AGAGGTCATT 
ACGCCTTCAG AGGAGATGAA CAGAATCAAT GAAATGGACC TAAAGCTGGG GAACGTGGAC 
ACAAAACTGG GAGACGGTTT GGCCGAGCAT CTGGAAGTGG GCAGCATTTT CACTTTCCGC 
GTCACCGTTC TGCAGGCAAG CGGCATCCCG GCCGAATACG CAGACATCTT CTGCCAGTTC 
AATTTCCTCC ACCGTCACGA TGAGGCGTTC AGCACTGAGC CTCTGAAGAA CACTGGCAGA 
GGAGCCCCGC TGGGCTTTTA CCACGTACAG AATATCTCAG TGGAGGTGAC AGAGTCCTTC 
ATTGAGTACA TAAAGACCAA GCCAATTGTA TTTGAAGTGT TTGGCCATTA CCAGCAACAC 
CCACTGCACA TTCATGGACA GGATGTGGTC AGCCCTTCTC AGCCTTCCAG AAAGTACTAT 
CCTCCACCAA TGCCTCTGTC TAAACCAGAT CACGCTCGTA AGATTGAGCT TATCCGTTAC 
CTGATGAGCG GGTATGTTCA TGGTCAGGTG TTGGACACAT TGTCAGAGCA TGCCAACGCT 
TTGGCCTCCA CCGCCGTGGC CAGTCTGGAG GAAGGGGCAT ATGAGCTGTG CCACTTCCCC 
TTCCTCCCCA TACCCAACTG CCCCGTCTTC AGAGTGCCCC GACACGACGT GCCAGCCACC 
AAACTGAACA CCATCACCAA GTCCAACCTG GGTCAGTGTG TGAGCAAGTA CGACTTGCTG 
GCCTGGTTTG AGATCAGCGA ACTGGAGCCC ACTGGAGAGT ATATTCCAGC AGTAGTGGAT 
CATACAGGAG GTCTTCCCTG TCATGGCACA TACCTGCTGC ACCAGGGCAT CCAGCGCAGA 
ATAACAGTGA CTCTGATCCA TGAGAAGGGA AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG 
CTGGTGGTCG GTCGAATCAG GAATAAGCCA GAAGTGGATG ATTCAGCCGC AGACGCCGTC 
TTGTCTCTCA ACATCATCTC AGCTAAAAAC CTCAAATCCT CCCACAACTC CAGCAGCCTT 
TGTATTGATA GCGATATTTC TAGGACGTTC TACCGGTTTG AGGCCGTGTG GGACAGTTCT 
CTGCACAACT CTCTCCTACT GAACCGCGTC ACTCCATACG GAGAGAAGAT CTACATGACC 
CTGTCTGCCT ATCTGGAGCT CGATCACTGC ATCCAGCCTG CTATCATCAC CAAGGACATC 
TGTATGGTCT TCTACTCCAG AGATGCCAAG ATCTCGCCTC CTCGCTCGCT GAGAAACTTG 
TTTGGCAGCG GCTACTCCAA AACCCCGGAC TGTAATAAAG TGACTGGAGT CTATGAGCTG 
AGCTTGTGCA AGATAGCAGA CACCGGAAGC CCAGGTATGC AGCGGCGCAG ACGGAAGGTT 
CTGGACACGT CAGTGGCATA TGTGCGTGGA GAGGAGAATC TGGCCGGCTG GAGACCCCGA 
GGAGACAGTC TGATCCTGGA GCACCAGTGG GAGCTGGACA AAATGGAGCA GCTGCATGAG 
GTGGAAAAGA CGCGTCACTT GCTTCTGCTG CGTGAGAAGC TGGGAGAAAC ACCTCCACCT 
AAATCTCTGA GCGAGTCTCT GTCCCCCAGC CTGAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CCCAGATCTC CTCCACAACT TTCGAGAGTG CCATCACCCC CAGCGAAAGC 
AGCGGCTATG ACTCCACGGA CATCGAGAGC TTGGTGGATC GAGAGAAGGA GCTGGCCACT 
AAGTGCCTCC GTCTCCTGAC ACACACTTTC AACAGTGAAT ACAACCAAAT GTGCAACAGC 
ATTAGTGACT GCAAGTTTTC AGATATCTCT CCTATGGGTC GGGATCCATC AGTCAGTAGT 
TTCAGCAGTG CCACCCTCAC ACCCTCCTCC ACCTGCCCCT CGCTCTCAGA CTCCAGGACC 
CCTGAAGCAA ACTCTCGTGC AACCAGCCCC TCAGGCTCAG ACTATGAGAA CTTCCCCATG 
GTGCCCATTC TGGAGACGTC CTACCTGGCC CGCGCCGGCA AACACGAGTT CCTCAACCTT 
GTGCCTAACA TAGAGGAAAT GAGGCCTGGG TCTGTGGTGT CCAAGAAGGG CATCTTGAAT 
TTCATGGAGC CCCGCTCGAA CACCTGGGTG AAGCACTTTG TGGTGGTGCG TCGGCCGTAT 
GTGTTCATCT ACAACAATGA CAAGGACCCT GTGGAGAGAG GAGTGCTGAA CTTATCCACT 
GCACAGGTGG AGTACAGTGA AGACCAGCAG GCCATGCTCA AGACGCCTCA TACATTTGCC 
ATGTGCACCA AACACAGAGG AATCCTGCTT CAGGCCAACA ATGACAAAGA CATGAACGAC 
TGGCTGTATG CCTTTAACCC ACTGCTAGCA GGAACAATAA GGTCGAAGCT TGCAAGGAGA 
CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296817.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295092.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.

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CloneID	ODa40266
Clone ID Related Accession (Same CDS sequence)	XM_009296817.2 , XM_009296817.3
Accession Version	XM_009296817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGTTT GGTCCTGATA GACCAACGTC ACTGTCCATT 
GAGGACTTTA GTGTTTATTC TTCCAATCAG CTTTTCGTTG AAAGGCCCTG TGAAGTCTAT 
ACTGACTTTA ATGGGGTGTT TTCACCCAAA AAGACGCCTC ACCTGGTGAA CCTGAACGAG 
GATCCTTTGA TGTCAGAATG TCTGCTCTAT TATATCAAAG ATGGAATAAC AAGGGTGGGC 
CAGGCAGATG CTGAAAGAAG ACAGGATATC GTTCTAAGTG GAGCTCACAT TAAAGAGGAG 
CACTGTATTT TCCGCAGTGA GAGGAATGCC AATGGACATG TGATTGTCAC GTTGGAGCCA 
TGTGAGGGAT CAGAAACATA TGTAAATGGT AAACGTGTGA ATTCTGCGGT CCAGCTGCGC 
TCAGGTAACC GCATTATCAT GGGAAAGAAC CACGTGTTCC GGTTTAACCA CCCTGAGCAG 
GCTCGCGCTG AGCGTGAGAA GACCCCGTCA GCAGAGACAC CTGTAGAACC TGTTGACTGG 
ACTTTTGCCC AAAGAGAACT GCTGGAGAAA CAGGGTATCG ACATGAAGCA AGAGATGGAG 
AAGAGGCTTA CAGAAATGGA GATCCTGTAC AAAAAAGAGA AGGAGGAAGC GGACCAGCTA 
CTGGAGCAAC AGAGACTGGT GTATGAGAGC AAACTGCAAG AGCTGCAGAA ACAAGTGGAG 
ACTCGTTCTT TGGCTGCAGA GACTCCAGAT GAGGAGGAAG AAGAAGAGGA GGAGGATGAA 
GTGCCCTGGA CCCAGCATAA ATATGAGCTG GCTCAGTGGG CTTTCAGGAA GTGGAGATAC 
CACCAGTTCA CCTCCCTCAG AGACCAATTA TGGGGCAATG CTGTGTACCT CAAAGAAGCC 
AATGCCATCA GTGTGGAGCT CAAGAAAAAG GTCCAGTTCC AGTTCGTCTT GCTCACAGAC 
ACCCTCTATT CTCCACTGCC CCCGGAGCTG CTGCCTCCAG AGCCAGAGAA AGAGCGAGAC 
GCCAGGCCTT TTCCGAGAAC AGTGGTGGCT GTGGAGGTTC AGGACCTGAA GAATGGAGCC 
ACTCACTACT GGTCCCTGGA GAAGCTGAAG CAGAGGCTGG ACCAGATGCG AGAGATGTAC 
GACCGTGCTG GCGAAATGGC CTCGTCCAAT CAGGGAGATG ATGGGGAGGG TGCGTTGACC 
GGCAGTGACC CGTTCTATGA CCGATTTCAT TGGTTTAAGC TGGTTGGGAG CTCACCTATT 
TTTCATGGCT GTGTGAACGA GCGTCTGGCC GACCGCACAC CATCGCCCAC CTTCTCCACC 
ACCGATTCGG AGATCACTGA GCTGGCCGAC GAGCGACAGA GCGAGATGGA GGACTTTATG 
GATGACGAGG CTTTCGTGGA TGACACCAGC TCAGATGCTG GAACCGAGGA GGGCTCGGAC 
ATCTTCAGTG ATGGCCAGGA CCCCTTCTAT GATCGTTCTC CATGGTTCAT CCTGGTGGGC 
AGAGCCTTCG TGTACCTGAG TAACCTGCTG TACCCTGTTC CTCTGGTGCA TCGAGTCGCT 
GTAGTGACAG AGAAAGGAGA TGTGCGAGGA TTTCTGCGTG TGGGGGTCCA GGCCATCGCC 
GCGGATGAAG AGGCACCTGA TTATGGGTCT GGTGTGCGTC AGTCAGGAAC TGCTAAGATT 
TCCTTTGATA ATGAGTATTT TAAAAAGAAT GACTTCACCT CAGTGGCCAT GACCCGCTCT 
GGTCTCTCTC TGGAGGAGCT GCGCATTGTA GAAGGTCAAG GTCAGAGTTC AGAGGTCATT 
ACGCCTTCAG AGGAGATGAA CAGAATCAAT GAAATGGACC TAAAGCTGGG GAACGTGGAC 
ACAAAACTGG GAGACGGTTT GGCCGAGCAT CTGGAAGTGG GCAGCATTTT CACTTTCCGC 
GTCACCGTTC TGCAGGCAAG CGGCATCCCG GCCGAATACG CAGACATCTT CTGCCAGTTC 
AATTTCCTCC ACCGTCACGA TGAGGCGTTC AGCACTGAGC CTCTGAAGAA CACTGGCAGA 
GGAGCCCCGC TGGGCTTTTA CCACGTACAG AATATCTCAG TGGAGGTGAC AGAGTCCTTC 
ATTGAGTACA TAAAGACCAA GCCAATTGTA TTTGAAGTGT TTGGCCATTA CCAGCAACAC 
CCACTGCACA TTCATGGACA GGATGTGGTC AGCCCTTCTC AGCCTTCCAG AAAGTACTAT 
CCTCCACCAA TGCCTCTGTC TAAACCAGAT CACGCTCGTA AGATTGAGCT TATCCGTTAC 
CTGATGAGCG GGTATGTTCA TGGTCAGGTG TTGGACACAT TGTCAGAGCA TGCCAACGCT 
TTGGCCTCCA CCGCCGTGGC CAGTCTGGAG GAAGGGGCAT ATGAGCTGTG CCACTTCCCC 
TTCCTCCCCA TACCCAACTG CCCCGTCTTC AGAGTGCCCC GACACGACGT GCCAGCCACC 
AAACTGAACA CCATCACCAA GTCCAACCTG GGTCAGTGTG TGAGCAAGTA CGACTTGCTG 
GCCTGGTTTG AGATCAGCGA ACTGGAGCCC ACTGGAGAGT ATATTCCAGC AGTAGTGGAT 
CATACAGGAG GTCTTCCCTG TCATGGCACA TACCTGCTGC ACCAGGGCAT CCAGCGCAGA 
ATAACAGTGA CTCTGATCCA TGAGAAGGGA AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG 
CTGGTGGTCG GTCGAATCAG GAATAAGCCA GAAGTGGATG ATTCAGCCGC AGACGCCGTC 
TTGTCTCTCA ACATCATCTC AGCTAAAAAC CTCAAATCCT CCCACAACTC CAGCAGCCTT 
TGTATTGATA GCGATATTTC TAGGACGTTC TACCGGTTTG AGGCCGTGTG GGACAGTTCT 
CTGCACAACT CTCTCCTACT GAACCGCGTC ACTCCATACG GAGAGAAGAT CTACATGACC 
CTGTCTGCCT ATCTGGAGCT CGATCACTGC ATCCAGCCTG CTATCATCAC CAAGGACATC 
TGTATGGTCT TCTACTCCAG AGATGCCAAG ATCTCGCCTC CTCGCTCGCT GAGAAACTTG 
TTTGGCAGCG GCTACTCCAA AACCCCGGAC TGTAATAAAG TGACTGGAGT CTATGAGCTG 
AGCTTGTGCA AGATAGCAGA CACCGGAAGC CCAGGTATGC AGCGGCGCAG ACGGAAGGTT 
CTGGACACGT CAGTGGCATA TGTGCGTGGA GAGGAGAATC TGGCCGGCTG GAGACCCCGA 
GGAGACAGTC TGATCCTGGA GCACCAGTGG GAGCTGGACA AAATGGAGCA GCTGCATGAG 
GTGGAAAAGA CGCGTCACTT GCTTCTGCTG CGTGAGAAGC TGGGAGAAAC ACCTCCACCT 
AAATCTCTGA GCGAGTCTCT GTCCCCCAGC CTGAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CCCAGATCTC CTCCACAACT TTCGAGAGTG CCATCACCCC CAGCGAAAGC 
AGCGGCTATG ACTCCACGGA CATCGAGAGC TTGGTGGATC GAGAGAAGGA GCTGGCCACT 
AAGTGCCTCC GTCTCCTGAC ACACACTTTC AACAGTGAAT ACAACCAAAT GTGCAACAGC 
ATTAGTGACT GCAAGTTTTC AGATATCTCT CCTATGGGTC GGGATCCATC AGTCAGTAGT 
TTCAGCAGTG CCACCCTCAC ACCCTCCTCC ACCTGCCCCT CGCTCTCAGA CTCCAGGACC 
CCTGAAGCAA ACTCTCGTGC AACCAGCCCC TCAGGCTCAG ACTATGAGAA CTTCCCCATG 
GTGCCCATTC TGGAGACGTC CTACCTGGCC CGCGCCGGCA AACACGAGTT CCTCAACCTT 
GTGCCTAACA TAGAGGAAAT GAGGCCTGGG TCTGTGGTGT CCAAGAAGGG CATCTTGAAT 
TTCATGGAGC CCCGCTCGAA CACCTGGGTG AAGCACTTTG TGGTGGTGCG TCGGCCGTAT 
GTGTTCATCT ACAACAATGA CAAGGACCCT GTGGAGAGAG GAGTGCTGAA CTTATCCACT 
GCACAGGTGG AGTACAGTGA AGACCAGCAG GCCATGCTCA AGACGCCTCA TACATTTGCC 
ATGTGCACCA AACACAGAGG AATCCTGCTT CAGGCCAACA ATGACAAAGA CATGAACGAC 
TGGCTGTATG CCTTTAACCC ACTGCTAGCA GGAACAATAA GGTCGAAGCT TGCAAGGAGA 
CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295092.1
CDS	320..5983
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296817.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296817.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGTTT GGTCCTGATA GACCAACGTC ACTGTCCATT 
GAGGACTTTA GTGTTTATTC TTCCAATCAG CTTTTCGTTG AAAGGCCCTG TGAAGTCTAT 
ACTGACTTTA ATGGGGTGTT TTCACCCAAA AAGACGCCTC ACCTGGTGAA CCTGAACGAG 
GATCCTTTGA TGTCAGAATG TCTGCTCTAT TATATCAAAG ATGGAATAAC AAGGGTGGGC 
CAGGCAGATG CTGAAAGAAG ACAGGATATC GTTCTAAGTG GAGCTCACAT TAAAGAGGAG 
CACTGTATTT TCCGCAGTGA GAGGAATGCC AATGGACATG TGATTGTCAC GTTGGAGCCA 
TGTGAGGGAT CAGAAACATA TGTAAATGGT AAACGTGTGA ATTCTGCGGT CCAGCTGCGC 
TCAGGTAACC GCATTATCAT GGGAAAGAAC CACGTGTTCC GGTTTAACCA CCCTGAGCAG 
GCTCGCGCTG AGCGTGAGAA GACCCCGTCA GCAGAGACAC CTGTAGAACC TGTTGACTGG 
ACTTTTGCCC AAAGAGAACT GCTGGAGAAA CAGGGTATCG ACATGAAGCA AGAGATGGAG 
AAGAGGCTTA CAGAAATGGA GATCCTGTAC AAAAAAGAGA AGGAGGAAGC GGACCAGCTA 
CTGGAGCAAC AGAGACTGGT GTATGAGAGC AAACTGCAAG AGCTGCAGAA ACAAGTGGAG 
ACTCGTTCTT TGGCTGCAGA GACTCCAGAT GAGGAGGAAG AAGAAGAGGA GGAGGATGAA 
GTGCCCTGGA CCCAGCATAA ATATGAGCTG GCTCAGTGGG CTTTCAGGAA GTGGAGATAC 
CACCAGTTCA CCTCCCTCAG AGACCAATTA TGGGGCAATG CTGTGTACCT CAAAGAAGCC 
AATGCCATCA GTGTGGAGCT CAAGAAAAAG GTCCAGTTCC AGTTCGTCTT GCTCACAGAC 
ACCCTCTATT CTCCACTGCC CCCGGAGCTG CTGCCTCCAG AGCCAGAGAA AGAGCGAGAC 
GCCAGGCCTT TTCCGAGAAC AGTGGTGGCT GTGGAGGTTC AGGACCTGAA GAATGGAGCC 
ACTCACTACT GGTCCCTGGA GAAGCTGAAG CAGAGGCTGG ACCAGATGCG AGAGATGTAC 
GACCGTGCTG GCGAAATGGC CTCGTCCAAT CAGGGAGATG ATGGGGAGGG TGCGTTGACC 
GGCAGTGACC CGTTCTATGA CCGATTTCAT TGGTTTAAGC TGGTTGGGAG CTCACCTATT 
TTTCATGGCT GTGTGAACGA GCGTCTGGCC GACCGCACAC CATCGCCCAC CTTCTCCACC 
ACCGATTCGG AGATCACTGA GCTGGCCGAC GAGCGACAGA GCGAGATGGA GGACTTTATG 
GATGACGAGG CTTTCGTGGA TGACACCAGC TCAGATGCTG GAACCGAGGA GGGCTCGGAC 
ATCTTCAGTG ATGGCCAGGA CCCCTTCTAT GATCGTTCTC CATGGTTCAT CCTGGTGGGC 
AGAGCCTTCG TGTACCTGAG TAACCTGCTG TACCCTGTTC CTCTGGTGCA TCGAGTCGCT 
GTAGTGACAG AGAAAGGAGA TGTGCGAGGA TTTCTGCGTG TGGGGGTCCA GGCCATCGCC 
GCGGATGAAG AGGCACCTGA TTATGGGTCT GGTGTGCGTC AGTCAGGAAC TGCTAAGATT 
TCCTTTGATA ATGAGTATTT TAAAAAGAAT GACTTCACCT CAGTGGCCAT GACCCGCTCT 
GGTCTCTCTC TGGAGGAGCT GCGCATTGTA GAAGGTCAAG GTCAGAGTTC AGAGGTCATT 
ACGCCTTCAG AGGAGATGAA CAGAATCAAT GAAATGGACC TAAAGCTGGG GAACGTGGAC 
ACAAAACTGG GAGACGGTTT GGCCGAGCAT CTGGAAGTGG GCAGCATTTT CACTTTCCGC 
GTCACCGTTC TGCAGGCAAG CGGCATCCCG GCCGAATACG CAGACATCTT CTGCCAGTTC 
AATTTCCTCC ACCGTCACGA TGAGGCGTTC AGCACTGAGC CTCTGAAGAA CACTGGCAGA 
GGAGCCCCGC TGGGCTTTTA CCACGTACAG AATATCTCAG TGGAGGTGAC AGAGTCCTTC 
ATTGAGTACA TAAAGACCAA GCCAATTGTA TTTGAAGTGT TTGGCCATTA CCAGCAACAC 
CCACTGCACA TTCATGGACA GGATGTGGTC AGCCCTTCTC AGCCTTCCAG AAAGTACTAT 
CCTCCACCAA TGCCTCTGTC TAAACCAGAT CACGCTCGTA AGATTGAGCT TATCCGTTAC 
CTGATGAGCG GGTATGTTCA TGGTCAGGTG TTGGACACAT TGTCAGAGCA TGCCAACGCT 
TTGGCCTCCA CCGCCGTGGC CAGTCTGGAG GAAGGGGCAT ATGAGCTGTG CCACTTCCCC 
TTCCTCCCCA TACCCAACTG CCCCGTCTTC AGAGTGCCCC GACACGACGT GCCAGCCACC 
AAACTGAACA CCATCACCAA GTCCAACCTG GGTCAGTGTG TGAGCAAGTA CGACTTGCTG 
GCCTGGTTTG AGATCAGCGA ACTGGAGCCC ACTGGAGAGT ATATTCCAGC AGTAGTGGAT 
CATACAGGAG GTCTTCCCTG TCATGGCACA TACCTGCTGC ACCAGGGCAT CCAGCGCAGA 
ATAACAGTGA CTCTGATCCA TGAGAAGGGA AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG 
CTGGTGGTCG GTCGAATCAG GAATAAGCCA GAAGTGGATG ATTCAGCCGC AGACGCCGTC 
TTGTCTCTCA ACATCATCTC AGCTAAAAAC CTCAAATCCT CCCACAACTC CAGCAGCCTT 
TGTATTGATA GCGATATTTC TAGGACGTTC TACCGGTTTG AGGCCGTGTG GGACAGTTCT 
CTGCACAACT CTCTCCTACT GAACCGCGTC ACTCCATACG GAGAGAAGAT CTACATGACC 
CTGTCTGCCT ATCTGGAGCT CGATCACTGC ATCCAGCCTG CTATCATCAC CAAGGACATC 
TGTATGGTCT TCTACTCCAG AGATGCCAAG ATCTCGCCTC CTCGCTCGCT GAGAAACTTG 
TTTGGCAGCG GCTACTCCAA AACCCCGGAC TGTAATAAAG TGACTGGAGT CTATGAGCTG 
AGCTTGTGCA AGATAGCAGA CACCGGAAGC CCAGGTATGC AGCGGCGCAG ACGGAAGGTT 
CTGGACACGT CAGTGGCATA TGTGCGTGGA GAGGAGAATC TGGCCGGCTG GAGACCCCGA 
GGAGACAGTC TGATCCTGGA GCACCAGTGG GAGCTGGACA AAATGGAGCA GCTGCATGAG 
GTGGAAAAGA CGCGTCACTT GCTTCTGCTG CGTGAGAAGC TGGGAGAAAC ACCTCCACCT 
AAATCTCTGA GCGAGTCTCT GTCCCCCAGC CTGAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CCCAGATCTC CTCCACAACT TTCGAGAGTG CCATCACCCC CAGCGAAAGC 
AGCGGCTATG ACTCCACGGA CATCGAGAGC TTGGTGGATC GAGAGAAGGA GCTGGCCACT 
AAGTGCCTCC GTCTCCTGAC ACACACTTTC AACAGTGAAT ACAACCAAAT GTGCAACAGC 
ATTAGTGACT GCAAGTTTTC AGATATCTCT CCTATGGGTC GGGATCCATC AGTCAGTAGT 
TTCAGCAGTG CCACCCTCAC ACCCTCCTCC ACCTGCCCCT CGCTCTCAGA CTCCAGGACC 
CCTGAAGCAA ACTCTCGTGC AACCAGCCCC TCAGGCTCAG ACTATGAGAA CTTCCCCATG 
GTGCCCATTC TGGAGACGTC CTACCTGGCC CGCGCCGGCA AACACGAGTT CCTCAACCTT 
GTGCCTAACA TAGAGGAAAT GAGGCCTGGG TCTGTGGTGT CCAAGAAGGG CATCTTGAAT 
TTCATGGAGC CCCGCTCGAA CACCTGGGTG AAGCACTTTG TGGTGGTGCG TCGGCCGTAT 
GTGTTCATCT ACAACAATGA CAAGGACCCT GTGGAGAGAG GAGTGCTGAA CTTATCCACT 
GCACAGGTGG AGTACAGTGA AGACCAGCAG GCCATGCTCA AGACGCCTCA TACATTTGCC 
ATGTGCACCA AACACAGAGG AATCCTGCTT CAGGCCAACA ATGACAAAGA CATGAACGAC 
TGGCTGTATG CCTTTAACCC ACTGCTAGCA GGAACAATAA GGTCGAAGCT TGCAAGGAGA 
CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296818.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295093.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.

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CloneID	ODa40267
Clone ID Related Accession (Same CDS sequence)	XM_009296818.3 , XM_009296818.2
Accession Version	XM_009296818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGCTCAC CTATTTTTCA TGGCTGTGTG 
AACGAGCGTC TGGCCGACCG CACACCATCG CCCACCTTCT CCACCACCGA TTCGGAGATC 
ACTGAGCTGG CCGACGAGCG ACAGAGCGAG ATGGAGGACT TTATGGATGA CGAGGCTTTC 
GTGGATGACA CCAGCTCAGA TGCTGGAACC GAGGAGGGCT CGGACATCTT CAGTGATGGC 
CAGGACCCCT TCTATGATCG TTCTCCATGG TTCATCCTGG TGGGCAGAGC CTTCGTGTAC 
CTGAGTAACC TGCTGTACCC TGTTCCTCTG GTGCATCGAG TCGCTGTAGT GACAGAGAAA 
GGAGATGTGC GAGGATTTCT GCGTGTGGGG GTCCAGGCCA TCGCCGCGGA TGAAGAGGCA 
CCTGATTATG GGTCTGGTGT GCGTCAGTCA GGAACTGCTA AGATTTCCTT TGATAATGAG 
TATTTTAAAA AGAATGACTT CACCTCAGTG GCCATGACCC GCTCTGGTCT CTCTCTGGAG 
GAGCTGCGCA TTGTAGAAGG TCAAGGTCAG AGTTCAGAGG TCATTACGCC TTCAGAGGAG 
ATGAACAGAA TCAATGAAAT GGACCTAAAG CTGGGGAACG TGGACACAAA ACTGGGAGAC 
GGTTTGGCCG AGCATCTGGA AGTGGGCAGC ATTTTCACTT TCCGCGTCAC CGTTCTGCAG 
GCAAGCGGCA TCCCGGCCGA ATACGCAGAC ATCTTCTGCC AGTTCAATTT CCTCCACCGT 
CACGATGAGG CGTTCAGCAC TGAGCCTCTG AAGAACACTG GCAGAGGAGC CCCGCTGGGC 
TTTTACCACG TACAGAATAT CTCAGTGGAG GTGACAGAGT CCTTCATTGA GTACATAAAG 
ACCAAGCCAA TTGTATTTGA AGTGTTTGGC CATTACCAGC AACACCCACT GCACATTCAT 
GGACAGGATG TGGTCAGCCC TTCTCAGCCT TCCAGAAAGT ACTATCCTCC ACCAATGCCT 
CTGTCTAAAC CAGATCACGC TCGTAAGATT GAGCTTATCC GTTACCTGAT GAGCGGGTAT 
GTTCATGGTC AGGTGTTGGA CACATTGTCA GAGCATGCCA ACGCTTTGGC CTCCACCGCC 
GTGGCCAGTC TGGAGGAAGG GGCATATGAG CTGTGCCACT TCCCCTTCCT CCCCATACCC 
AACTGCCCCG TCTTCAGAGT GCCCCGACAC GACGTGCCAG CCACCAAACT GAACACCATC 
ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC AAGTACGACT TGCTGGCCTG GTTTGAGATC 
AGCGAACTGG AGCCCACTGG AGAGTATATT CCAGCAGTAG TGGATCATAC AGGAGGTCTT 
CCCTGTCATG GCACATACCT GCTGCACCAG GGCATCCAGC GCAGAATAAC AGTGACTCTG 
ATCCATGAGA AGGGAAGTGA GCTGCACTGG AAGGACGTCC GAGAGCTGGT GGTCGGTCGA 
ATCAGGAATA AGCCAGAAGT GGATGATTCA GCCGCAGACG CCGTCTTGTC TCTCAACATC 
ATCTCAGCTA AAAACCTCAA ATCCTCCCAC AACTCCAGCA GCCTTTGTAT TGATAGCGAT 
ATTTCTAGGA CGTTCTACCG GTTTGAGGCC GTGTGGGACA GTTCTCTGCA CAACTCTCTC 
CTACTGAACC GCGTCACTCC ATACGGAGAG AAGATCTACA TGACCCTGTC TGCCTATCTG 
GAGCTCGATC ACTGCATCCA GCCTGCTATC ATCACCAAGG ACATCTGTAT GGTCTTCTAC 
TCCAGAGATG CCAAGATCTC GCCTCCTCGC TCGCTGAGAA ACTTGTTTGG CAGCGGCTAC 
TCCAAAACCC CGGACTGTAA TAAAGTGACT GGAGTCTATG AGCTGAGCTT GTGCAAGATA 
GCAGACACCG GAAGCCCAGG TATGCAGCGG CGCAGACGGA AGGTTCTGGA CACGTCAGTG 
GCATATGTGC GTGGAGAGGA GAATCTGGCC GGCTGGAGAC CCCGAGGAGA CAGTCTGATC 
CTGGAGCACC AGTGGGAGCT GGACAAAATG GAGCAGCTGC ATGAGGTGGA AAAGACGCGT 
CACTTGCTTC TGCTGCGTGA GAAGCTGGGA GAAACACCTC CACCTAAATC TCTGAGCGAG 
TCTCTGTCCC CCAGCCTGAG CAGCGGGACC CTCAGCACCT CCACCAGCAT CTCCTCCCAG 
ATCTCCTCCA CAACTTTCGA GAGTGCCATC ACCCCCAGCG AAAGCAGCGG CTATGACTCC 
ACGGACATCG AGAGCTTGGT GGATCGAGAG AAGGAGCTGG CCACTAAGTG CCTCCGTCTC 
CTGACACACA CTTTCAACAG TGAATACAAC CAAATGTGCA ACAGCATTAG TGACTGCAAG 
TTTTCAGATA TCTCTCCTAT GGGTCGGGAT CCATCAGTCA GTAGTTTCAG CAGTGCCACC 
CTCACACCCT CCTCCACCTG CCCCTCGCTC TCAGACTCCA GGACCCCTGA AGCAAACTCT 
CGTGCAACCA GCCCCTCAGG CTCAGACTAT GAGAACTTCC CCATGGTGCC CATTCTGGAG 
ACGTCCTACC TGGCCCGCGC CGGCAAACAC GAGTTCCTCA ACCTTGTGCC TAACATAGAG 
GAAATGAGGC CTGGGTCTGT GGTGTCCAAG AAGGGCATCT TGAATTTCAT GGAGCCCCGC 
TCGAACACCT GGGTGAAGCA CTTTGTGGTG GTGCGTCGGC CGTATGTGTT CATCTACAAC 
AATGACAAGG ACCCTGTGGA GAGAGGAGTG CTGAACTTAT CCACTGCACA GGTGGAGTAC 
AGTGAAGACC AGCAGGCCAT GCTCAAGACG CCTCATACAT TTGCCATGTG CACCAAACAC 
AGAGGAATCC TGCTTCAGGC CAACAATGAC AAAGACATGA ACGACTGGCT GTATGCCTTT 
AACCCACTGC TAGCAGGAAC AATAAGGTCG AAGCTTGCAA GGAGACGGTC TGGTGTGATG 
AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295093.1
CDS	320..5968
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296818.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296818.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGCTCAC CTATTTTTCA TGGCTGTGTG 
AACGAGCGTC TGGCCGACCG CACACCATCG CCCACCTTCT CCACCACCGA TTCGGAGATC 
ACTGAGCTGG CCGACGAGCG ACAGAGCGAG ATGGAGGACT TTATGGATGA CGAGGCTTTC 
GTGGATGACA CCAGCTCAGA TGCTGGAACC GAGGAGGGCT CGGACATCTT CAGTGATGGC 
CAGGACCCCT TCTATGATCG TTCTCCATGG TTCATCCTGG TGGGCAGAGC CTTCGTGTAC 
CTGAGTAACC TGCTGTACCC TGTTCCTCTG GTGCATCGAG TCGCTGTAGT GACAGAGAAA 
GGAGATGTGC GAGGATTTCT GCGTGTGGGG GTCCAGGCCA TCGCCGCGGA TGAAGAGGCA 
CCTGATTATG GGTCTGGTGT GCGTCAGTCA GGAACTGCTA AGATTTCCTT TGATAATGAG 
TATTTTAAAA AGAATGACTT CACCTCAGTG GCCATGACCC GCTCTGGTCT CTCTCTGGAG 
GAGCTGCGCA TTGTAGAAGG TCAAGGTCAG AGTTCAGAGG TCATTACGCC TTCAGAGGAG 
ATGAACAGAA TCAATGAAAT GGACCTAAAG CTGGGGAACG TGGACACAAA ACTGGGAGAC 
GGTTTGGCCG AGCATCTGGA AGTGGGCAGC ATTTTCACTT TCCGCGTCAC CGTTCTGCAG 
GCAAGCGGCA TCCCGGCCGA ATACGCAGAC ATCTTCTGCC AGTTCAATTT CCTCCACCGT 
CACGATGAGG CGTTCAGCAC TGAGCCTCTG AAGAACACTG GCAGAGGAGC CCCGCTGGGC 
TTTTACCACG TACAGAATAT CTCAGTGGAG GTGACAGAGT CCTTCATTGA GTACATAAAG 
ACCAAGCCAA TTGTATTTGA AGTGTTTGGC CATTACCAGC AACACCCACT GCACATTCAT 
GGACAGGATG TGGTCAGCCC TTCTCAGCCT TCCAGAAAGT ACTATCCTCC ACCAATGCCT 
CTGTCTAAAC CAGATCACGC TCGTAAGATT GAGCTTATCC GTTACCTGAT GAGCGGGTAT 
GTTCATGGTC AGGTGTTGGA CACATTGTCA GAGCATGCCA ACGCTTTGGC CTCCACCGCC 
GTGGCCAGTC TGGAGGAAGG GGCATATGAG CTGTGCCACT TCCCCTTCCT CCCCATACCC 
AACTGCCCCG TCTTCAGAGT GCCCCGACAC GACGTGCCAG CCACCAAACT GAACACCATC 
ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC AAGTACGACT TGCTGGCCTG GTTTGAGATC 
AGCGAACTGG AGCCCACTGG AGAGTATATT CCAGCAGTAG TGGATCATAC AGGAGGTCTT 
CCCTGTCATG GCACATACCT GCTGCACCAG GGCATCCAGC GCAGAATAAC AGTGACTCTG 
ATCCATGAGA AGGGAAGTGA GCTGCACTGG AAGGACGTCC GAGAGCTGGT GGTCGGTCGA 
ATCAGGAATA AGCCAGAAGT GGATGATTCA GCCGCAGACG CCGTCTTGTC TCTCAACATC 
ATCTCAGCTA AAAACCTCAA ATCCTCCCAC AACTCCAGCA GCCTTTGTAT TGATAGCGAT 
ATTTCTAGGA CGTTCTACCG GTTTGAGGCC GTGTGGGACA GTTCTCTGCA CAACTCTCTC 
CTACTGAACC GCGTCACTCC ATACGGAGAG AAGATCTACA TGACCCTGTC TGCCTATCTG 
GAGCTCGATC ACTGCATCCA GCCTGCTATC ATCACCAAGG ACATCTGTAT GGTCTTCTAC 
TCCAGAGATG CCAAGATCTC GCCTCCTCGC TCGCTGAGAA ACTTGTTTGG CAGCGGCTAC 
TCCAAAACCC CGGACTGTAA TAAAGTGACT GGAGTCTATG AGCTGAGCTT GTGCAAGATA 
GCAGACACCG GAAGCCCAGG TATGCAGCGG CGCAGACGGA AGGTTCTGGA CACGTCAGTG 
GCATATGTGC GTGGAGAGGA GAATCTGGCC GGCTGGAGAC CCCGAGGAGA CAGTCTGATC 
CTGGAGCACC AGTGGGAGCT GGACAAAATG GAGCAGCTGC ATGAGGTGGA AAAGACGCGT 
CACTTGCTTC TGCTGCGTGA GAAGCTGGGA GAAACACCTC CACCTAAATC TCTGAGCGAG 
TCTCTGTCCC CCAGCCTGAG CAGCGGGACC CTCAGCACCT CCACCAGCAT CTCCTCCCAG 
ATCTCCTCCA CAACTTTCGA GAGTGCCATC ACCCCCAGCG AAAGCAGCGG CTATGACTCC 
ACGGACATCG AGAGCTTGGT GGATCGAGAG AAGGAGCTGG CCACTAAGTG CCTCCGTCTC 
CTGACACACA CTTTCAACAG TGAATACAAC CAAATGTGCA ACAGCATTAG TGACTGCAAG 
TTTTCAGATA TCTCTCCTAT GGGTCGGGAT CCATCAGTCA GTAGTTTCAG CAGTGCCACC 
CTCACACCCT CCTCCACCTG CCCCTCGCTC TCAGACTCCA GGACCCCTGA AGCAAACTCT 
CGTGCAACCA GCCCCTCAGG CTCAGACTAT GAGAACTTCC CCATGGTGCC CATTCTGGAG 
ACGTCCTACC TGGCCCGCGC CGGCAAACAC GAGTTCCTCA ACCTTGTGCC TAACATAGAG 
GAAATGAGGC CTGGGTCTGT GGTGTCCAAG AAGGGCATCT TGAATTTCAT GGAGCCCCGC 
TCGAACACCT GGGTGAAGCA CTTTGTGGTG GTGCGTCGGC CGTATGTGTT CATCTACAAC 
AATGACAAGG ACCCTGTGGA GAGAGGAGTG CTGAACTTAT CCACTGCACA GGTGGAGTAC 
AGTGAAGACC AGCAGGCCAT GCTCAAGACG CCTCATACAT TTGCCATGTG CACCAAACAC 
AGAGGAATCC TGCTTCAGGC CAACAATGAC AAAGACATGA ACGACTGGCT GTATGCCTTT 
AACCCACTGC TAGCAGGAAC AATAAGGTCG AAGCTTGCAA GGAGACGGTC TGGTGTGATG 
AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296818.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295093.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.

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CloneID	ODa40267
Clone ID Related Accession (Same CDS sequence)	XM_009296818.3 , XM_009296818.2
Accession Version	XM_009296818.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGCTCAC CTATTTTTCA TGGCTGTGTG 
AACGAGCGTC TGGCCGACCG CACACCATCG CCCACCTTCT CCACCACCGA TTCGGAGATC 
ACTGAGCTGG CCGACGAGCG ACAGAGCGAG ATGGAGGACT TTATGGATGA CGAGGCTTTC 
GTGGATGACA CCAGCTCAGA TGCTGGAACC GAGGAGGGCT CGGACATCTT CAGTGATGGC 
CAGGACCCCT TCTATGATCG TTCTCCATGG TTCATCCTGG TGGGCAGAGC CTTCGTGTAC 
CTGAGTAACC TGCTGTACCC TGTTCCTCTG GTGCATCGAG TCGCTGTAGT GACAGAGAAA 
GGAGATGTGC GAGGATTTCT GCGTGTGGGG GTCCAGGCCA TCGCCGCGGA TGAAGAGGCA 
CCTGATTATG GGTCTGGTGT GCGTCAGTCA GGAACTGCTA AGATTTCCTT TGATAATGAG 
TATTTTAAAA AGAATGACTT CACCTCAGTG GCCATGACCC GCTCTGGTCT CTCTCTGGAG 
GAGCTGCGCA TTGTAGAAGG TCAAGGTCAG AGTTCAGAGG TCATTACGCC TTCAGAGGAG 
ATGAACAGAA TCAATGAAAT GGACCTAAAG CTGGGGAACG TGGACACAAA ACTGGGAGAC 
GGTTTGGCCG AGCATCTGGA AGTGGGCAGC ATTTTCACTT TCCGCGTCAC CGTTCTGCAG 
GCAAGCGGCA TCCCGGCCGA ATACGCAGAC ATCTTCTGCC AGTTCAATTT CCTCCACCGT 
CACGATGAGG CGTTCAGCAC TGAGCCTCTG AAGAACACTG GCAGAGGAGC CCCGCTGGGC 
TTTTACCACG TACAGAATAT CTCAGTGGAG GTGACAGAGT CCTTCATTGA GTACATAAAG 
ACCAAGCCAA TTGTATTTGA AGTGTTTGGC CATTACCAGC AACACCCACT GCACATTCAT 
GGACAGGATG TGGTCAGCCC TTCTCAGCCT TCCAGAAAGT ACTATCCTCC ACCAATGCCT 
CTGTCTAAAC CAGATCACGC TCGTAAGATT GAGCTTATCC GTTACCTGAT GAGCGGGTAT 
GTTCATGGTC AGGTGTTGGA CACATTGTCA GAGCATGCCA ACGCTTTGGC CTCCACCGCC 
GTGGCCAGTC TGGAGGAAGG GGCATATGAG CTGTGCCACT TCCCCTTCCT CCCCATACCC 
AACTGCCCCG TCTTCAGAGT GCCCCGACAC GACGTGCCAG CCACCAAACT GAACACCATC 
ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC AAGTACGACT TGCTGGCCTG GTTTGAGATC 
AGCGAACTGG AGCCCACTGG AGAGTATATT CCAGCAGTAG TGGATCATAC AGGAGGTCTT 
CCCTGTCATG GCACATACCT GCTGCACCAG GGCATCCAGC GCAGAATAAC AGTGACTCTG 
ATCCATGAGA AGGGAAGTGA GCTGCACTGG AAGGACGTCC GAGAGCTGGT GGTCGGTCGA 
ATCAGGAATA AGCCAGAAGT GGATGATTCA GCCGCAGACG CCGTCTTGTC TCTCAACATC 
ATCTCAGCTA AAAACCTCAA ATCCTCCCAC AACTCCAGCA GCCTTTGTAT TGATAGCGAT 
ATTTCTAGGA CGTTCTACCG GTTTGAGGCC GTGTGGGACA GTTCTCTGCA CAACTCTCTC 
CTACTGAACC GCGTCACTCC ATACGGAGAG AAGATCTACA TGACCCTGTC TGCCTATCTG 
GAGCTCGATC ACTGCATCCA GCCTGCTATC ATCACCAAGG ACATCTGTAT GGTCTTCTAC 
TCCAGAGATG CCAAGATCTC GCCTCCTCGC TCGCTGAGAA ACTTGTTTGG CAGCGGCTAC 
TCCAAAACCC CGGACTGTAA TAAAGTGACT GGAGTCTATG AGCTGAGCTT GTGCAAGATA 
GCAGACACCG GAAGCCCAGG TATGCAGCGG CGCAGACGGA AGGTTCTGGA CACGTCAGTG 
GCATATGTGC GTGGAGAGGA GAATCTGGCC GGCTGGAGAC CCCGAGGAGA CAGTCTGATC 
CTGGAGCACC AGTGGGAGCT GGACAAAATG GAGCAGCTGC ATGAGGTGGA AAAGACGCGT 
CACTTGCTTC TGCTGCGTGA GAAGCTGGGA GAAACACCTC CACCTAAATC TCTGAGCGAG 
TCTCTGTCCC CCAGCCTGAG CAGCGGGACC CTCAGCACCT CCACCAGCAT CTCCTCCCAG 
ATCTCCTCCA CAACTTTCGA GAGTGCCATC ACCCCCAGCG AAAGCAGCGG CTATGACTCC 
ACGGACATCG AGAGCTTGGT GGATCGAGAG AAGGAGCTGG CCACTAAGTG CCTCCGTCTC 
CTGACACACA CTTTCAACAG TGAATACAAC CAAATGTGCA ACAGCATTAG TGACTGCAAG 
TTTTCAGATA TCTCTCCTAT GGGTCGGGAT CCATCAGTCA GTAGTTTCAG CAGTGCCACC 
CTCACACCCT CCTCCACCTG CCCCTCGCTC TCAGACTCCA GGACCCCTGA AGCAAACTCT 
CGTGCAACCA GCCCCTCAGG CTCAGACTAT GAGAACTTCC CCATGGTGCC CATTCTGGAG 
ACGTCCTACC TGGCCCGCGC CGGCAAACAC GAGTTCCTCA ACCTTGTGCC TAACATAGAG 
GAAATGAGGC CTGGGTCTGT GGTGTCCAAG AAGGGCATCT TGAATTTCAT GGAGCCCCGC 
TCGAACACCT GGGTGAAGCA CTTTGTGGTG GTGCGTCGGC CGTATGTGTT CATCTACAAC 
AATGACAAGG ACCCTGTGGA GAGAGGAGTG CTGAACTTAT CCACTGCACA GGTGGAGTAC 
AGTGAAGACC AGCAGGCCAT GCTCAAGACG CCTCATACAT TTGCCATGTG CACCAAACAC 
AGAGGAATCC TGCTTCAGGC CAACAATGAC AAAGACATGA ACGACTGGCT GTATGCCTTT 
AACCCACTGC TAGCAGGAAC AATAAGGTCG AAGCTTGCAA GGAGACGGTC TGGTGTGATG 
AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295093.1
CDS	319..5967
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296818.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296818.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGAC GCCTCACCTG GTGAACCTGA ACGAGGATCC TTTGATGTCA 
GAATGTCTGC TCTATTATAT CAAAGATGGA ATAACAAGGG TGGGCCAGGC AGATGCTGAA 
AGAAGACAGG ATATCGTTCT AAGTGGAGCT CACATTAAAG AGGAGCACTG TATTTTCCGC 
AGTGAGAGGA ATGCCAATGG ACATGTGATT GTCACGTTGG AGCCATGTGA GGGATCAGAA 
ACATATGTAA ATGGTAAACG TGTGAATTCT GCGGTCCAGC TGCGCTCAGG TAACCGCATT 
ATCATGGGAA AGAACCACGT GTTCCGGTTT AACCACCCTG AGCAGGCTCG CGCTGAGCGT 
GAGAAGACCC CGTCAGCAGA GACACCTGTA GAACCTGTTG ACTGGACTTT TGCCCAAAGA 
GAACTGCTGG AGAAACAGGG TATCGACATG AAGCAAGAGA TGGAGAAGAG GCTTACAGAA 
ATGGAGATCC TGTACAAAAA AGAGAAGGAG GAAGCGGACC AGCTACTGGA GCAACAGAGA 
CTGGTGTATG AGAGCAAACT GCAAGAGCTG CAGAAACAAG TGGAGACTCG TTCTTTGGCT 
GCAGAGACTC CAGATGAGGA GGAAGAAGAA GAGGAGGAGG ATGAAGTGCC CTGGACCCAG 
CATAAATATG AGCTGGCTCA GTGGGCTTTC AGGAAGTGGA GATACCACCA GTTCACCTCC 
CTCAGAGACC AATTATGGGG CAATGCTGTG TACCTCAAAG AAGCCAATGC CATCAGTGTG 
GAGCTCAAGA AAAAGGTCCA GTTCCAGTTC GTCTTGCTCA CAGACACCCT CTATTCTCCA 
CTGCCCCCGG AGCTGCTGCC TCCAGAGCCA GAGAAAGAGC GAGACGCCAG GCCTTTTCCG 
AGAACAGTGG TGGCTGTGGA GGTTCAGGAC CTGAAGAATG GAGCCACTCA CTACTGGTCC 
CTGGAGAAGC TGAAGCAGAG GCTGGACCAG ATGCGAGAGA TGTACGACCG TGCTGGCGAA 
ATGGCCTCGT CCAATCAGGG AGATGATGGG GAGGGTGCGT TGACCGGCAG TGACCCGTTC 
TATGACCGAT TTCATTGGTT TAAGCTGGTT GGGAGCTCAC CTATTTTTCA TGGCTGTGTG 
AACGAGCGTC TGGCCGACCG CACACCATCG CCCACCTTCT CCACCACCGA TTCGGAGATC 
ACTGAGCTGG CCGACGAGCG ACAGAGCGAG ATGGAGGACT TTATGGATGA CGAGGCTTTC 
GTGGATGACA CCAGCTCAGA TGCTGGAACC GAGGAGGGCT CGGACATCTT CAGTGATGGC 
CAGGACCCCT TCTATGATCG TTCTCCATGG TTCATCCTGG TGGGCAGAGC CTTCGTGTAC 
CTGAGTAACC TGCTGTACCC TGTTCCTCTG GTGCATCGAG TCGCTGTAGT GACAGAGAAA 
GGAGATGTGC GAGGATTTCT GCGTGTGGGG GTCCAGGCCA TCGCCGCGGA TGAAGAGGCA 
CCTGATTATG GGTCTGGTGT GCGTCAGTCA GGAACTGCTA AGATTTCCTT TGATAATGAG 
TATTTTAAAA AGAATGACTT CACCTCAGTG GCCATGACCC GCTCTGGTCT CTCTCTGGAG 
GAGCTGCGCA TTGTAGAAGG TCAAGGTCAG AGTTCAGAGG TCATTACGCC TTCAGAGGAG 
ATGAACAGAA TCAATGAAAT GGACCTAAAG CTGGGGAACG TGGACACAAA ACTGGGAGAC 
GGTTTGGCCG AGCATCTGGA AGTGGGCAGC ATTTTCACTT TCCGCGTCAC CGTTCTGCAG 
GCAAGCGGCA TCCCGGCCGA ATACGCAGAC ATCTTCTGCC AGTTCAATTT CCTCCACCGT 
CACGATGAGG CGTTCAGCAC TGAGCCTCTG AAGAACACTG GCAGAGGAGC CCCGCTGGGC 
TTTTACCACG TACAGAATAT CTCAGTGGAG GTGACAGAGT CCTTCATTGA GTACATAAAG 
ACCAAGCCAA TTGTATTTGA AGTGTTTGGC CATTACCAGC AACACCCACT GCACATTCAT 
GGACAGGATG TGGTCAGCCC TTCTCAGCCT TCCAGAAAGT ACTATCCTCC ACCAATGCCT 
CTGTCTAAAC CAGATCACGC TCGTAAGATT GAGCTTATCC GTTACCTGAT GAGCGGGTAT 
GTTCATGGTC AGGTGTTGGA CACATTGTCA GAGCATGCCA ACGCTTTGGC CTCCACCGCC 
GTGGCCAGTC TGGAGGAAGG GGCATATGAG CTGTGCCACT TCCCCTTCCT CCCCATACCC 
AACTGCCCCG TCTTCAGAGT GCCCCGACAC GACGTGCCAG CCACCAAACT GAACACCATC 
ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC AAGTACGACT TGCTGGCCTG GTTTGAGATC 
AGCGAACTGG AGCCCACTGG AGAGTATATT CCAGCAGTAG TGGATCATAC AGGAGGTCTT 
CCCTGTCATG GCACATACCT GCTGCACCAG GGCATCCAGC GCAGAATAAC AGTGACTCTG 
ATCCATGAGA AGGGAAGTGA GCTGCACTGG AAGGACGTCC GAGAGCTGGT GGTCGGTCGA 
ATCAGGAATA AGCCAGAAGT GGATGATTCA GCCGCAGACG CCGTCTTGTC TCTCAACATC 
ATCTCAGCTA AAAACCTCAA ATCCTCCCAC AACTCCAGCA GCCTTTGTAT TGATAGCGAT 
ATTTCTAGGA CGTTCTACCG GTTTGAGGCC GTGTGGGACA GTTCTCTGCA CAACTCTCTC 
CTACTGAACC GCGTCACTCC ATACGGAGAG AAGATCTACA TGACCCTGTC TGCCTATCTG 
GAGCTCGATC ACTGCATCCA GCCTGCTATC ATCACCAAGG ACATCTGTAT GGTCTTCTAC 
TCCAGAGATG CCAAGATCTC GCCTCCTCGC TCGCTGAGAA ACTTGTTTGG CAGCGGCTAC 
TCCAAAACCC CGGACTGTAA TAAAGTGACT GGAGTCTATG AGCTGAGCTT GTGCAAGATA 
GCAGACACCG GAAGCCCAGG TATGCAGCGG CGCAGACGGA AGGTTCTGGA CACGTCAGTG 
GCATATGTGC GTGGAGAGGA GAATCTGGCC GGCTGGAGAC CCCGAGGAGA CAGTCTGATC 
CTGGAGCACC AGTGGGAGCT GGACAAAATG GAGCAGCTGC ATGAGGTGGA AAAGACGCGT 
CACTTGCTTC TGCTGCGTGA GAAGCTGGGA GAAACACCTC CACCTAAATC TCTGAGCGAG 
TCTCTGTCCC CCAGCCTGAG CAGCGGGACC CTCAGCACCT CCACCAGCAT CTCCTCCCAG 
ATCTCCTCCA CAACTTTCGA GAGTGCCATC ACCCCCAGCG AAAGCAGCGG CTATGACTCC 
ACGGACATCG AGAGCTTGGT GGATCGAGAG AAGGAGCTGG CCACTAAGTG CCTCCGTCTC 
CTGACACACA CTTTCAACAG TGAATACAAC CAAATGTGCA ACAGCATTAG TGACTGCAAG 
TTTTCAGATA TCTCTCCTAT GGGTCGGGAT CCATCAGTCA GTAGTTTCAG CAGTGCCACC 
CTCACACCCT CCTCCACCTG CCCCTCGCTC TCAGACTCCA GGACCCCTGA AGCAAACTCT 
CGTGCAACCA GCCCCTCAGG CTCAGACTAT GAGAACTTCC CCATGGTGCC CATTCTGGAG 
ACGTCCTACC TGGCCCGCGC CGGCAAACAC GAGTTCCTCA ACCTTGTGCC TAACATAGAG 
GAAATGAGGC CTGGGTCTGT GGTGTCCAAG AAGGGCATCT TGAATTTCAT GGAGCCCCGC 
TCGAACACCT GGGTGAAGCA CTTTGTGGTG GTGCGTCGGC CGTATGTGTT CATCTACAAC 
AATGACAAGG ACCCTGTGGA GAGAGGAGTG CTGAACTTAT CCACTGCACA GGTGGAGTAC 
AGTGAAGACC AGCAGGCCAT GCTCAAGACG CCTCATACAT TTGCCATGTG CACCAAACAC 
AGAGGAATCC TGCTTCAGGC CAACAATGAC AAAGACATGA ACGACTGGCT GTATGCCTTT 
AACCCACTGC TAGCAGGAAC AATAAGGTCG AAGCTTGCAA GGAGACGGTC TGGTGTGATG 
AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296820.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295095.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.

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CloneID	ODa40268
Clone ID Related Accession (Same CDS sequence)	XM_009296820.3 , XM_009296820.2
Accession Version	XM_009296820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGCCTT TGTATTGATA GCGATATTTC TAGGACGTTC 
TACCGGTTTG AGGCCGTGTG GGACAGTTCT CTGCACAACT CTCTCCTACT GAACCGCGTC 
ACTCCATACG GAGAGAAGAT CTACATGACC CTGTCTGCCT ATCTGGAGCT CGATCACTGC 
ATCCAGCCTG CTATCATCAC CAAGGACATC TGTATGGTCT TCTACTCCAG AGATGCCAAG 
ATCTCGCCTC CTCGCTCGCT GAGAAACTTG TTTGGCAGCG GCTACTCCAA AACCCCGGAC 
TGTAATAAAG TGACTGGAGT CTATGAGCTG AGCTTGTGCA AGATAGCAGA CACCGGAAGC 
CCAGGTATGC AGCGGCGCAG ACGGAAGGTT CTGGACACGT CAGTGGCATA TGTGCGTGGA 
GAGGAGAATC TGGCCGGCTG GAGACCCCGA GGAGACAGTC TGATCCTGGA GCACCAGTGG 
GAGCTGGACA AAATGGAGCA GCTGCATGAG GTGGAAAAGA CGCGTCACTT GCTTCTGCTG 
CGTGAGAAGC TGGGAGAAAC ACCTCCACCT AAATCTCTGA GCGAGTCTCT GTCCCCCAGC 
CTGAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CCCAGATCTC CTCCACAACT 
TTCGAGAGTG CCATCACCCC CAGCGAAAGC AGCGGCTATG ACTCCACGGA CATCGAGAGC 
TTGGTGGATC GAGAGAAGGA GCTGGCCACT AAGTGCCTCC GTCTCCTGAC ACACACTTTC 
AACAGTGAAT ACAACCAAAT GTGCAACAGC ATTAGTGACT GCAAGTTTTC AGATATCTCT 
CCTATGGGTC GGGATCCATC AGTCAGTAGT TTCAGCAGTG CCACCCTCAC ACCCTCCTCC 
ACCTGCCCCT CGCTCTCAGA CTCCAGGACC CCTGAAGCAA ACTCTCGTGC AACCAGCCCC 
TCAGGCTCAG ACTATGAGAA CTTCCCCATG GTGCCCATTC TGGAGACGTC CTACCTGGCC 
CGCGCCGGCA AACACGAGTT CCTCAACCTT GTGCCTAACA TAGAGGAAAT GAGGCCTGGG 
TCTGTGGTGT CCAAGAAGGG CATCTTGAAT TTCATGGAGC CCCGCTCGAA CACCTGGGTG 
AAGCACTTTG TGGTGGTGCG TCGGCCGTAT GTGTTCATCT ACAACAATGA CAAGGACCCT 
GTGGAGAGAG GAGTGCTGAA CTTATCCACT GCACAGGTGG AGTACAGTGA AGACCAGCAG 
GCCATGCTCA AGACGCCTCA TACATTTGCC ATGTGCACCA AACACAGAGG AATCCTGCTT 
CAGGCCAACA ATGACAAAGA CATGAACGAC TGGCTGTATG CCTTTAACCC ACTGCTAGCA 
GGAACAATAA GGTCGAAGCT TGCAAGGAGA CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295095.1
CDS	320..5893
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296820.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296820.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGCCTT TGTATTGATA GCGATATTTC TAGGACGTTC 
TACCGGTTTG AGGCCGTGTG GGACAGTTCT CTGCACAACT CTCTCCTACT GAACCGCGTC 
ACTCCATACG GAGAGAAGAT CTACATGACC CTGTCTGCCT ATCTGGAGCT CGATCACTGC 
ATCCAGCCTG CTATCATCAC CAAGGACATC TGTATGGTCT TCTACTCCAG AGATGCCAAG 
ATCTCGCCTC CTCGCTCGCT GAGAAACTTG TTTGGCAGCG GCTACTCCAA AACCCCGGAC 
TGTAATAAAG TGACTGGAGT CTATGAGCTG AGCTTGTGCA AGATAGCAGA CACCGGAAGC 
CCAGGTATGC AGCGGCGCAG ACGGAAGGTT CTGGACACGT CAGTGGCATA TGTGCGTGGA 
GAGGAGAATC TGGCCGGCTG GAGACCCCGA GGAGACAGTC TGATCCTGGA GCACCAGTGG 
GAGCTGGACA AAATGGAGCA GCTGCATGAG GTGGAAAAGA CGCGTCACTT GCTTCTGCTG 
CGTGAGAAGC TGGGAGAAAC ACCTCCACCT AAATCTCTGA GCGAGTCTCT GTCCCCCAGC 
CTGAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CCCAGATCTC CTCCACAACT 
TTCGAGAGTG CCATCACCCC CAGCGAAAGC AGCGGCTATG ACTCCACGGA CATCGAGAGC 
TTGGTGGATC GAGAGAAGGA GCTGGCCACT AAGTGCCTCC GTCTCCTGAC ACACACTTTC 
AACAGTGAAT ACAACCAAAT GTGCAACAGC ATTAGTGACT GCAAGTTTTC AGATATCTCT 
CCTATGGGTC GGGATCCATC AGTCAGTAGT TTCAGCAGTG CCACCCTCAC ACCCTCCTCC 
ACCTGCCCCT CGCTCTCAGA CTCCAGGACC CCTGAAGCAA ACTCTCGTGC AACCAGCCCC 
TCAGGCTCAG ACTATGAGAA CTTCCCCATG GTGCCCATTC TGGAGACGTC CTACCTGGCC 
CGCGCCGGCA AACACGAGTT CCTCAACCTT GTGCCTAACA TAGAGGAAAT GAGGCCTGGG 
TCTGTGGTGT CCAAGAAGGG CATCTTGAAT TTCATGGAGC CCCGCTCGAA CACCTGGGTG 
AAGCACTTTG TGGTGGTGCG TCGGCCGTAT GTGTTCATCT ACAACAATGA CAAGGACCCT 
GTGGAGAGAG GAGTGCTGAA CTTATCCACT GCACAGGTGG AGTACAGTGA AGACCAGCAG 
GCCATGCTCA AGACGCCTCA TACATTTGCC ATGTGCACCA AACACAGAGG AATCCTGCTT 
CAGGCCAACA ATGACAAAGA CATGAACGAC TGGCTGTATG CCTTTAACCC ACTGCTAGCA 
GGAACAATAA GGTCGAAGCT TGCAAGGAGA CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296820.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295095.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.

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CloneID	ODa40268
Clone ID Related Accession (Same CDS sequence)	XM_009296820.3 , XM_009296820.2
Accession Version	XM_009296820.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGCCTT TGTATTGATA GCGATATTTC TAGGACGTTC 
TACCGGTTTG AGGCCGTGTG GGACAGTTCT CTGCACAACT CTCTCCTACT GAACCGCGTC 
ACTCCATACG GAGAGAAGAT CTACATGACC CTGTCTGCCT ATCTGGAGCT CGATCACTGC 
ATCCAGCCTG CTATCATCAC CAAGGACATC TGTATGGTCT TCTACTCCAG AGATGCCAAG 
ATCTCGCCTC CTCGCTCGCT GAGAAACTTG TTTGGCAGCG GCTACTCCAA AACCCCGGAC 
TGTAATAAAG TGACTGGAGT CTATGAGCTG AGCTTGTGCA AGATAGCAGA CACCGGAAGC 
CCAGGTATGC AGCGGCGCAG ACGGAAGGTT CTGGACACGT CAGTGGCATA TGTGCGTGGA 
GAGGAGAATC TGGCCGGCTG GAGACCCCGA GGAGACAGTC TGATCCTGGA GCACCAGTGG 
GAGCTGGACA AAATGGAGCA GCTGCATGAG GTGGAAAAGA CGCGTCACTT GCTTCTGCTG 
CGTGAGAAGC TGGGAGAAAC ACCTCCACCT AAATCTCTGA GCGAGTCTCT GTCCCCCAGC 
CTGAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CCCAGATCTC CTCCACAACT 
TTCGAGAGTG CCATCACCCC CAGCGAAAGC AGCGGCTATG ACTCCACGGA CATCGAGAGC 
TTGGTGGATC GAGAGAAGGA GCTGGCCACT AAGTGCCTCC GTCTCCTGAC ACACACTTTC 
AACAGTGAAT ACAACCAAAT GTGCAACAGC ATTAGTGACT GCAAGTTTTC AGATATCTCT 
CCTATGGGTC GGGATCCATC AGTCAGTAGT TTCAGCAGTG CCACCCTCAC ACCCTCCTCC 
ACCTGCCCCT CGCTCTCAGA CTCCAGGACC CCTGAAGCAA ACTCTCGTGC AACCAGCCCC 
TCAGGCTCAG ACTATGAGAA CTTCCCCATG GTGCCCATTC TGGAGACGTC CTACCTGGCC 
CGCGCCGGCA AACACGAGTT CCTCAACCTT GTGCCTAACA TAGAGGAAAT GAGGCCTGGG 
TCTGTGGTGT CCAAGAAGGG CATCTTGAAT TTCATGGAGC CCCGCTCGAA CACCTGGGTG 
AAGCACTTTG TGGTGGTGCG TCGGCCGTAT GTGTTCATCT ACAACAATGA CAAGGACCCT 
GTGGAGAGAG GAGTGCTGAA CTTATCCACT GCACAGGTGG AGTACAGTGA AGACCAGCAG 
GCCATGCTCA AGACGCCTCA TACATTTGCC ATGTGCACCA AACACAGAGG AATCCTGCTT 
CAGGCCAACA ATGACAAAGA CATGAACGAC TGGCTGTATG CCTTTAACCC ACTGCTAGCA 
GGAACAATAA GGTCGAAGCT TGCAAGGAGA CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295095.1
CDS	319..5892
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296820.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296820.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGCCTT TGTATTGATA GCGATATTTC TAGGACGTTC 
TACCGGTTTG AGGCCGTGTG GGACAGTTCT CTGCACAACT CTCTCCTACT GAACCGCGTC 
ACTCCATACG GAGAGAAGAT CTACATGACC CTGTCTGCCT ATCTGGAGCT CGATCACTGC 
ATCCAGCCTG CTATCATCAC CAAGGACATC TGTATGGTCT TCTACTCCAG AGATGCCAAG 
ATCTCGCCTC CTCGCTCGCT GAGAAACTTG TTTGGCAGCG GCTACTCCAA AACCCCGGAC 
TGTAATAAAG TGACTGGAGT CTATGAGCTG AGCTTGTGCA AGATAGCAGA CACCGGAAGC 
CCAGGTATGC AGCGGCGCAG ACGGAAGGTT CTGGACACGT CAGTGGCATA TGTGCGTGGA 
GAGGAGAATC TGGCCGGCTG GAGACCCCGA GGAGACAGTC TGATCCTGGA GCACCAGTGG 
GAGCTGGACA AAATGGAGCA GCTGCATGAG GTGGAAAAGA CGCGTCACTT GCTTCTGCTG 
CGTGAGAAGC TGGGAGAAAC ACCTCCACCT AAATCTCTGA GCGAGTCTCT GTCCCCCAGC 
CTGAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CCCAGATCTC CTCCACAACT 
TTCGAGAGTG CCATCACCCC CAGCGAAAGC AGCGGCTATG ACTCCACGGA CATCGAGAGC 
TTGGTGGATC GAGAGAAGGA GCTGGCCACT AAGTGCCTCC GTCTCCTGAC ACACACTTTC 
AACAGTGAAT ACAACCAAAT GTGCAACAGC ATTAGTGACT GCAAGTTTTC AGATATCTCT 
CCTATGGGTC GGGATCCATC AGTCAGTAGT TTCAGCAGTG CCACCCTCAC ACCCTCCTCC 
ACCTGCCCCT CGCTCTCAGA CTCCAGGACC CCTGAAGCAA ACTCTCGTGC AACCAGCCCC 
TCAGGCTCAG ACTATGAGAA CTTCCCCATG GTGCCCATTC TGGAGACGTC CTACCTGGCC 
CGCGCCGGCA AACACGAGTT CCTCAACCTT GTGCCTAACA TAGAGGAAAT GAGGCCTGGG 
TCTGTGGTGT CCAAGAAGGG CATCTTGAAT TTCATGGAGC CCCGCTCGAA CACCTGGGTG 
AAGCACTTTG TGGTGGTGCG TCGGCCGTAT GTGTTCATCT ACAACAATGA CAAGGACCCT 
GTGGAGAGAG GAGTGCTGAA CTTATCCACT GCACAGGTGG AGTACAGTGA AGACCAGCAG 
GCCATGCTCA AGACGCCTCA TACATTTGCC ATGTGCACCA AACACAGAGG AATCCTGCTT 
CAGGCCAACA ATGACAAAGA CATGAACGAC TGGCTGTATG CCTTTAACCC ACTGCTAGCA 
GGAACAATAA GGTCGAAGCT TGCAAGGAGA CGGTCTGGTG TGATGAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296821.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295096.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.

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CloneID	ODa40269
Clone ID Related Accession (Same CDS sequence)	XM_009296821.2 , XM_009296821.3
Accession Version	XM_009296821.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGGACG TTCTACCGGT TTGAGGCCGT GTGGGACAGT 
TCTCTGCACA ACTCTCTCCT ACTGAACCGC GTCACTCCAT ACGGAGAGAA GATCTACATG 
ACCCTGTCTG CCTATCTGGA GCTCGATCAC TGCATCCAGC CTGCTATCAT CACCAAGGAC 
ATCTGTATGG TCTTCTACTC CAGAGATGCC AAGATCTCGC CTCCTCGCTC GCTGAGAAAC 
TTGTTTGGCA GCGGCTACTC CAAAACCCCG GACTGTAATA AAGTGACTGG AGTCTATGAG 
CTGAGCTTGT GCAAGATAGC AGACACCGGA AGCCCAGGTA TGCAGCGGCG CAGACGGAAG 
GTTCTGGACA CGTCAGTGGC ATATGTGCGT GGAGAGGAGA ATCTGGCCGG CTGGAGACCC 
CGAGGAGACA GTCTGATCCT GGAGCACCAG TGGGAGCTGG ACAAAATGGA GCAGCTGCAT 
GAGGTGGAAA AGACGCGTCA CTTGCTTCTG CTGCGTGAGA AGCTGGGAGA AACACCTCCA 
CCTAAATCTC TGAGCGAGTC TCTGTCCCCC AGCCTGAGCA GCGGGACCCT CAGCACCTCC 
ACCAGCATCT CCTCCCAGAT CTCCTCCACA ACTTTCGAGA GTGCCATCAC CCCCAGCGAA 
AGCAGCGGCT ATGACTCCAC GGACATCGAG AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC 
ACTAAGTGCC TCCGTCTCCT GACACACACT TTCAACAGTG AATACAACCA AATGTGCAAC 
AGCATTAGTG ACTGCAAGTT TTCAGATATC TCTCCTATGG GTCGGGATCC ATCAGTCAGT 
AGTTTCAGCA GTGCCACCCT CACACCCTCC TCCACCTGCC CCTCGCTCTC AGACTCCAGG 
ACCCCTGAAG CAAACTCTCG TGCAACCAGC CCCTCAGGCT CAGACTATGA GAACTTCCCC 
ATGGTGCCCA TTCTGGAGAC GTCCTACCTG GCCCGCGCCG GCAAACACGA GTTCCTCAAC 
CTTGTGCCTA ACATAGAGGA AATGAGGCCT GGGTCTGTGG TGTCCAAGAA GGGCATCTTG 
AATTTCATGG AGCCCCGCTC GAACACCTGG GTGAAGCACT TTGTGGTGGT GCGTCGGCCG 
TATGTGTTCA TCTACAACAA TGACAAGGAC CCTGTGGAGA GAGGAGTGCT GAACTTATCC 
ACTGCACAGG TGGAGTACAG TGAAGACCAG CAGGCCATGC TCAAGACGCC TCATACATTT 
GCCATGTGCA CCAAACACAG AGGAATCCTG CTTCAGGCCA ACAATGACAA AGACATGAAC 
GACTGGCTGT ATGCCTTTAA CCCACTGCTA GCAGGAACAA TAAGGTCGAA GCTTGCAAGG 
AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295096.1
CDS	319..5865
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296821.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296821.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGGACG TTCTACCGGT TTGAGGCCGT GTGGGACAGT 
TCTCTGCACA ACTCTCTCCT ACTGAACCGC GTCACTCCAT ACGGAGAGAA GATCTACATG 
ACCCTGTCTG CCTATCTGGA GCTCGATCAC TGCATCCAGC CTGCTATCAT CACCAAGGAC 
ATCTGTATGG TCTTCTACTC CAGAGATGCC AAGATCTCGC CTCCTCGCTC GCTGAGAAAC 
TTGTTTGGCA GCGGCTACTC CAAAACCCCG GACTGTAATA AAGTGACTGG AGTCTATGAG 
CTGAGCTTGT GCAAGATAGC AGACACCGGA AGCCCAGGTA TGCAGCGGCG CAGACGGAAG 
GTTCTGGACA CGTCAGTGGC ATATGTGCGT GGAGAGGAGA ATCTGGCCGG CTGGAGACCC 
CGAGGAGACA GTCTGATCCT GGAGCACCAG TGGGAGCTGG ACAAAATGGA GCAGCTGCAT 
GAGGTGGAAA AGACGCGTCA CTTGCTTCTG CTGCGTGAGA AGCTGGGAGA AACACCTCCA 
CCTAAATCTC TGAGCGAGTC TCTGTCCCCC AGCCTGAGCA GCGGGACCCT CAGCACCTCC 
ACCAGCATCT CCTCCCAGAT CTCCTCCACA ACTTTCGAGA GTGCCATCAC CCCCAGCGAA 
AGCAGCGGCT ATGACTCCAC GGACATCGAG AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC 
ACTAAGTGCC TCCGTCTCCT GACACACACT TTCAACAGTG AATACAACCA AATGTGCAAC 
AGCATTAGTG ACTGCAAGTT TTCAGATATC TCTCCTATGG GTCGGGATCC ATCAGTCAGT 
AGTTTCAGCA GTGCCACCCT CACACCCTCC TCCACCTGCC CCTCGCTCTC AGACTCCAGG 
ACCCCTGAAG CAAACTCTCG TGCAACCAGC CCCTCAGGCT CAGACTATGA GAACTTCCCC 
ATGGTGCCCA TTCTGGAGAC GTCCTACCTG GCCCGCGCCG GCAAACACGA GTTCCTCAAC 
CTTGTGCCTA ACATAGAGGA AATGAGGCCT GGGTCTGTGG TGTCCAAGAA GGGCATCTTG 
AATTTCATGG AGCCCCGCTC GAACACCTGG GTGAAGCACT TTGTGGTGGT GCGTCGGCCG 
TATGTGTTCA TCTACAACAA TGACAAGGAC CCTGTGGAGA GAGGAGTGCT GAACTTATCC 
ACTGCACAGG TGGAGTACAG TGAAGACCAG CAGGCCATGC TCAAGACGCC TCATACATTT 
GCCATGTGCA CCAAACACAG AGGAATCCTG CTTCAGGCCA ACAATGACAA AGACATGAAC 
GACTGGCTGT ATGCCTTTAA CCCACTGCTA GCAGGAACAA TAAGGTCGAA GCTTGCAAGG 
AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296821.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295096.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.

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CloneID	ODa40269
Clone ID Related Accession (Same CDS sequence)	XM_009296821.2 , XM_009296821.3
Accession Version	XM_009296821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGGACG TTCTACCGGT TTGAGGCCGT GTGGGACAGT 
TCTCTGCACA ACTCTCTCCT ACTGAACCGC GTCACTCCAT ACGGAGAGAA GATCTACATG 
ACCCTGTCTG CCTATCTGGA GCTCGATCAC TGCATCCAGC CTGCTATCAT CACCAAGGAC 
ATCTGTATGG TCTTCTACTC CAGAGATGCC AAGATCTCGC CTCCTCGCTC GCTGAGAAAC 
TTGTTTGGCA GCGGCTACTC CAAAACCCCG GACTGTAATA AAGTGACTGG AGTCTATGAG 
CTGAGCTTGT GCAAGATAGC AGACACCGGA AGCCCAGGTA TGCAGCGGCG CAGACGGAAG 
GTTCTGGACA CGTCAGTGGC ATATGTGCGT GGAGAGGAGA ATCTGGCCGG CTGGAGACCC 
CGAGGAGACA GTCTGATCCT GGAGCACCAG TGGGAGCTGG ACAAAATGGA GCAGCTGCAT 
GAGGTGGAAA AGACGCGTCA CTTGCTTCTG CTGCGTGAGA AGCTGGGAGA AACACCTCCA 
CCTAAATCTC TGAGCGAGTC TCTGTCCCCC AGCCTGAGCA GCGGGACCCT CAGCACCTCC 
ACCAGCATCT CCTCCCAGAT CTCCTCCACA ACTTTCGAGA GTGCCATCAC CCCCAGCGAA 
AGCAGCGGCT ATGACTCCAC GGACATCGAG AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC 
ACTAAGTGCC TCCGTCTCCT GACACACACT TTCAACAGTG AATACAACCA AATGTGCAAC 
AGCATTAGTG ACTGCAAGTT TTCAGATATC TCTCCTATGG GTCGGGATCC ATCAGTCAGT 
AGTTTCAGCA GTGCCACCCT CACACCCTCC TCCACCTGCC CCTCGCTCTC AGACTCCAGG 
ACCCCTGAAG CAAACTCTCG TGCAACCAGC CCCTCAGGCT CAGACTATGA GAACTTCCCC 
ATGGTGCCCA TTCTGGAGAC GTCCTACCTG GCCCGCGCCG GCAAACACGA GTTCCTCAAC 
CTTGTGCCTA ACATAGAGGA AATGAGGCCT GGGTCTGTGG TGTCCAAGAA GGGCATCTTG 
AATTTCATGG AGCCCCGCTC GAACACCTGG GTGAAGCACT TTGTGGTGGT GCGTCGGCCG 
TATGTGTTCA TCTACAACAA TGACAAGGAC CCTGTGGAGA GAGGAGTGCT GAACTTATCC 
ACTGCACAGG TGGAGTACAG TGAAGACCAG CAGGCCATGC TCAAGACGCC TCATACATTT 
GCCATGTGCA CCAAACACAG AGGAATCCTG CTTCAGGCCA ACAATGACAA AGACATGAAC 
GACTGGCTGT ATGCCTTTAA CCCACTGCTA GCAGGAACAA TAAGGTCGAA GCTTGCAAGG 
AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295096.1
CDS	320..5866
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296821.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296821.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GCTCACCTAT TTTTCATGGC 
TGTGTGAACG AGCGTCTGGC CGACCGCACA CCATCGCCCA CCTTCTCCAC CACCGATTCG 
GAGATCACTG AGCTGGCCGA CGAGCGACAG AGCGAGATGG AGGACTTTAT GGATGACGAG 
GCTTTCGTGG ATGACACCAG CTCAGATGCT GGAACCGAGG AGGGCTCGGA CATCTTCAGT 
GATGGCCAGG ACCCCTTCTA TGATCGTTCT CCATGGTTCA TCCTGGTGGG CAGAGCCTTC 
GTGTACCTGA GTAACCTGCT GTACCCTGTT CCTCTGGTGC ATCGAGTCGC TGTAGTGACA 
GAGAAAGGAG ATGTGCGAGG ATTTCTGCGT GTGGGGGTCC AGGCCATCGC CGCGGATGAA 
GAGGCACCTG ATTATGGGTC TGGTGTGCGT CAGTCAGGAA CTGCTAAGAT TTCCTTTGAT 
AATGAGTATT TTAAAAAGAA TGACTTCACC TCAGTGGCCA TGACCCGCTC TGGTCTCTCT 
CTGGAGGAGC TGCGCATTGT AGAAGGTCAA GGTCAGAGTT CAGAGGTCAT TACGCCTTCA 
GAGGAGATGA ACAGAATCAA TGAAATGGAC CTAAAGCTGG GGAACGTGGA CACAAAACTG 
GGAGACGGTT TGGCCGAGCA TCTGGAAGTG GGCAGCATTT TCACTTTCCG CGTCACCGTT 
CTGCAGGCAA GCGGCATCCC GGCCGAATAC GCAGACATCT TCTGCCAGTT CAATTTCCTC 
CACCGTCACG ATGAGGCGTT CAGCACTGAG CCTCTGAAGA ACACTGGCAG AGGAGCCCCG 
CTGGGCTTTT ACCACGTACA GAATATCTCA GTGGAGGTGA CAGAGTCCTT CATTGAGTAC 
ATAAAGACCA AGCCAATTGT ATTTGAAGTG TTTGGCCATT ACCAGCAACA CCCACTGCAC 
ATTCATGGAC AGGATGTGGT CAGCCCTTCT CAGCCTTCCA GAAAGTACTA TCCTCCACCA 
ATGCCTCTGT CTAAACCAGT GCCAGCCACC AAACTGAACA CCATCACCAA GTCCAACCTG 
GGTCAGTGTG TGAGCAAGTA CGACTTGCTG GCCTGGTTTG AGATCAGCGA ACTGGAGCCC 
ACTGGAGAGT ATATTCCAGC AGTAGTGGAT CATACAGGAG GTCTTCCCTG TCATGGCACA 
TACCTGCTGC ACCAGGGCAT CCAGCGCAGA ATAACAGTGA CTCTGATCCA TGAGAAGGGA 
AGTGAGCTGC ACTGGAAGGA CGTCCGAGAG CTGGTGGTCG GTCGAATCAG GAATAAGCCA 
GAAGTGGATG ATTCAGCCGC AGACGCCGTC TTGTCTCTCA ACATCATCTC AGCTAAAAAC 
CTCAAATCCT CCCACAACTC CAGCAGGACG TTCTACCGGT TTGAGGCCGT GTGGGACAGT 
TCTCTGCACA ACTCTCTCCT ACTGAACCGC GTCACTCCAT ACGGAGAGAA GATCTACATG 
ACCCTGTCTG CCTATCTGGA GCTCGATCAC TGCATCCAGC CTGCTATCAT CACCAAGGAC 
ATCTGTATGG TCTTCTACTC CAGAGATGCC AAGATCTCGC CTCCTCGCTC GCTGAGAAAC 
TTGTTTGGCA GCGGCTACTC CAAAACCCCG GACTGTAATA AAGTGACTGG AGTCTATGAG 
CTGAGCTTGT GCAAGATAGC AGACACCGGA AGCCCAGGTA TGCAGCGGCG CAGACGGAAG 
GTTCTGGACA CGTCAGTGGC ATATGTGCGT GGAGAGGAGA ATCTGGCCGG CTGGAGACCC 
CGAGGAGACA GTCTGATCCT GGAGCACCAG TGGGAGCTGG ACAAAATGGA GCAGCTGCAT 
GAGGTGGAAA AGACGCGTCA CTTGCTTCTG CTGCGTGAGA AGCTGGGAGA AACACCTCCA 
CCTAAATCTC TGAGCGAGTC TCTGTCCCCC AGCCTGAGCA GCGGGACCCT CAGCACCTCC 
ACCAGCATCT CCTCCCAGAT CTCCTCCACA ACTTTCGAGA GTGCCATCAC CCCCAGCGAA 
AGCAGCGGCT ATGACTCCAC GGACATCGAG AGCTTGGTGG ATCGAGAGAA GGAGCTGGCC 
ACTAAGTGCC TCCGTCTCCT GACACACACT TTCAACAGTG AATACAACCA AATGTGCAAC 
AGCATTAGTG ACTGCAAGTT TTCAGATATC TCTCCTATGG GTCGGGATCC ATCAGTCAGT 
AGTTTCAGCA GTGCCACCCT CACACCCTCC TCCACCTGCC CCTCGCTCTC AGACTCCAGG 
ACCCCTGAAG CAAACTCTCG TGCAACCAGC CCCTCAGGCT CAGACTATGA GAACTTCCCC 
ATGGTGCCCA TTCTGGAGAC GTCCTACCTG GCCCGCGCCG GCAAACACGA GTTCCTCAAC 
CTTGTGCCTA ACATAGAGGA AATGAGGCCT GGGTCTGTGG TGTCCAAGAA GGGCATCTTG 
AATTTCATGG AGCCCCGCTC GAACACCTGG GTGAAGCACT TTGTGGTGGT GCGTCGGCCG 
TATGTGTTCA TCTACAACAA TGACAAGGAC CCTGTGGAGA GAGGAGTGCT GAACTTATCC 
ACTGCACAGG TGGAGTACAG TGAAGACCAG CAGGCCATGC TCAAGACGCC TCATACATTT 
GCCATGTGCA CCAAACACAG AGGAATCCTG CTTCAGGCCA ACAATGACAA AGACATGAAC 
GACTGGCTGT ATGCCTTTAA CCCACTGCTA GCAGGAACAA TAAGGTCGAA GCTTGCAAGG 
AGACGGTCTG GTGTGATGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296822.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295097.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.

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CloneID	ODa40270
Clone ID Related Accession (Same CDS sequence)	XM_009296822.2 , XM_009296822.3
Accession Version	XM_009296822.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GAGCCTTCGT GTACCTGAGT 
AACCTGCTGT ACCCTGTTCC TCTGGTGCAT CGAGTCGCTG TAGTGACAGA GAAAGGAGAT 
GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG GCCATCGCCG CGGATGAAGA GGCACCTGAT 
TATGGGTCTG GTGTGCGTCA GTCAGGAACT GCTAAGATTT CCTTTGATAA TGAGTATTTT 
AAAAAGAATG ACTTCACCTC AGTGGCCATG ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG 
CGCATTGTAG AAGGTCAAGG TCAGAGTTCA GAGGTCATTA CGCCTTCAGA GGAGATGAAC 
AGAATCAATG AAATGGACCT AAAGCTGGGG AACGTGGACA CAAAACTGGG AGACGGTTTG 
GCCGAGCATC TGGAAGTGGG CAGCATTTTC ACTTTCCGCG TCACCGTTCT GCAGGCAAGC 
GGCATCCCGG CCGAATACGC AGACATCTTC TGCCAGTTCA ATTTCCTCCA CCGTCACGAT 
GAGGCGTTCA GCACTGAGCC TCTGAAGAAC ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC 
CACGTACAGA ATATCTCAGT GGAGGTGACA GAGTCCTTCA TTGAGTACAT AAAGACCAAG 
CCAATTGTAT TTGAAGTGTT TGGCCATTAC CAGCAACACC CACTGCACAT TCATGGACAG 
GATGTGGTCA GCCCTTCTCA GCCTTCCAGA AAGTACTATC CTCCACCAAT GCCTCTGTCT 
AAACCAGATC ACGCTCGTAA GATTGAGCTT ATCCGTTACC TGATGAGCGG GTATGTTCAT 
GGTCAGGTGT TGGACACATT GTCAGAGCAT GCCAACGCTT TGGCCTCCAC CGCCGTGGCC 
AGTCTGGAGG AAGGGGCATA TGAGCTGTGC CACTTCCCCT TCCTCCCCAT ACCCAACTGC 
CCCGTCTTCA GAGTGCCCCG ACACGACGTG CCAGCCACCA AACTGAACAC CATCACCAAG 
TCCAACCTGG GTCAGTGTGT GAGCAAGTAC GACTTGCTGG CCTGGTTTGA GATCAGCGAA 
CTGGAGCCCA CTGGAGAGTA TATTCCAGCA GTAGTGGATC ATACAGGAGG TCTTCCCTGT 
CATGGCACAT ACCTGCTGCA CCAGGGCATC CAGCGCAGAA TAACAGTGAC TCTGATCCAT 
GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC GTCCGAGAGC TGGTGGTCGG TCGAATCAGG 
AATAAGCCAG AAGTGGATGA TTCAGCCGCA GACGCCGTCT TGTCTCTCAA CATCATCTCA 
GCTAAAAACC TCAAATCCTC CCACAACTCC AGCAGCCTTT GTATTGATAG CGATATTTCT 
AGGACGTTCT ACCGGTTTGA GGCCGTGTGG GACAGTTCTC TGCACAACTC TCTCCTACTG 
AACCGCGTCA CTCCATACGG AGAGAAGATC TACATGACCC TGTCTGCCTA TCTGGAGCTC 
GATCACTGCA TCCAGCCTGC TATCATCACC AAGGACATCT GTATGGTCTT CTACTCCAGA 
GATGCCAAGA TCTCGCCTCC TCGCTCGCTG AGAAACTTGT TTGGCAGCGG CTACTCCAAA 
ACCCCGGACT GTAATAAAGT GACTGGAGTC TATGAGCTGA GCTTGTGCAA GATAGCAGAC 
ACCGGAAGCC CAGGTATGCA GCGGCGCAGA CGGAAGGTTC TGGACACGTC AGTGGCATAT 
GTGCGTGGAG AGGAGAATCT GGCCGGCTGG AGACCCCGAG GAGACAGTCT GATCCTGGAG 
CACCAGTGGG AGCTGGACAA AATGGAGCAG CTGCATGAGG TGGAAAAGAC GCGTCACTTG 
CTTCTGCTGC GTGAGAAGCT GGGAGAAACA CCTCCACCTA AATCTCTGAG CGAGTCTCTG 
TCCCCCAGCC TGAGCAGCGG GACCCTCAGC ACCTCCACCA GCATCTCCTC CCAGATCTCC 
TCCACAACTT TCGAGAGTGC CATCACCCCC AGCGAAAGCA GCGGCTATGA CTCCACGGAC 
ATCGAGAGCT TGGTGGATCG AGAGAAGGAG CTGGCCACTA AGTGCCTCCG TCTCCTGACA 
CACACTTTCA ACAGTGAATA CAACCAAATG TGCAACAGCA TTAGTGACTG CAAGTTTTCA 
GATATCTCTC CTATGGGTCG GGATCCATCA GTCAGTAGTT TCAGCAGTGC CACCCTCACA 
CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC TCCAGGACCC CTGAAGCAAA CTCTCGTGCA 
ACCAGCCCCT CAGGCTCAGA CTATGAGAAC TTCCCCATGG TGCCCATTCT GGAGACGTCC 
TACCTGGCCC GCGCCGGCAA ACACGAGTTC CTCAACCTTG TGCCTAACAT AGAGGAAATG 
AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC ATCTTGAATT TCATGGAGCC CCGCTCGAAC 
ACCTGGGTGA AGCACTTTGT GGTGGTGCGT CGGCCGTATG TGTTCATCTA CAACAATGAC 
AAGGACCCTG TGGAGAGAGG AGTGCTGAAC TTATCCACTG CACAGGTGGA GTACAGTGAA 
GACCAGCAGG CCATGCTCAA GACGCCTCAT ACATTTGCCA TGTGCACCAA ACACAGAGGA 
ATCCTGCTTC AGGCCAACAA TGACAAAGAC ATGAACGACT GGCTGTATGC CTTTAACCCA 
CTGCTAGCAG GAACAATAAG GTCGAAGCTT GCAAGGAGAC GGTCTGGTGT GATGAAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295097.1
CDS	319..5841
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296822.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296822.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GAGCCTTCGT GTACCTGAGT 
AACCTGCTGT ACCCTGTTCC TCTGGTGCAT CGAGTCGCTG TAGTGACAGA GAAAGGAGAT 
GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG GCCATCGCCG CGGATGAAGA GGCACCTGAT 
TATGGGTCTG GTGTGCGTCA GTCAGGAACT GCTAAGATTT CCTTTGATAA TGAGTATTTT 
AAAAAGAATG ACTTCACCTC AGTGGCCATG ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG 
CGCATTGTAG AAGGTCAAGG TCAGAGTTCA GAGGTCATTA CGCCTTCAGA GGAGATGAAC 
AGAATCAATG AAATGGACCT AAAGCTGGGG AACGTGGACA CAAAACTGGG AGACGGTTTG 
GCCGAGCATC TGGAAGTGGG CAGCATTTTC ACTTTCCGCG TCACCGTTCT GCAGGCAAGC 
GGCATCCCGG CCGAATACGC AGACATCTTC TGCCAGTTCA ATTTCCTCCA CCGTCACGAT 
GAGGCGTTCA GCACTGAGCC TCTGAAGAAC ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC 
CACGTACAGA ATATCTCAGT GGAGGTGACA GAGTCCTTCA TTGAGTACAT AAAGACCAAG 
CCAATTGTAT TTGAAGTGTT TGGCCATTAC CAGCAACACC CACTGCACAT TCATGGACAG 
GATGTGGTCA GCCCTTCTCA GCCTTCCAGA AAGTACTATC CTCCACCAAT GCCTCTGTCT 
AAACCAGATC ACGCTCGTAA GATTGAGCTT ATCCGTTACC TGATGAGCGG GTATGTTCAT 
GGTCAGGTGT TGGACACATT GTCAGAGCAT GCCAACGCTT TGGCCTCCAC CGCCGTGGCC 
AGTCTGGAGG AAGGGGCATA TGAGCTGTGC CACTTCCCCT TCCTCCCCAT ACCCAACTGC 
CCCGTCTTCA GAGTGCCCCG ACACGACGTG CCAGCCACCA AACTGAACAC CATCACCAAG 
TCCAACCTGG GTCAGTGTGT GAGCAAGTAC GACTTGCTGG CCTGGTTTGA GATCAGCGAA 
CTGGAGCCCA CTGGAGAGTA TATTCCAGCA GTAGTGGATC ATACAGGAGG TCTTCCCTGT 
CATGGCACAT ACCTGCTGCA CCAGGGCATC CAGCGCAGAA TAACAGTGAC TCTGATCCAT 
GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC GTCCGAGAGC TGGTGGTCGG TCGAATCAGG 
AATAAGCCAG AAGTGGATGA TTCAGCCGCA GACGCCGTCT TGTCTCTCAA CATCATCTCA 
GCTAAAAACC TCAAATCCTC CCACAACTCC AGCAGCCTTT GTATTGATAG CGATATTTCT 
AGGACGTTCT ACCGGTTTGA GGCCGTGTGG GACAGTTCTC TGCACAACTC TCTCCTACTG 
AACCGCGTCA CTCCATACGG AGAGAAGATC TACATGACCC TGTCTGCCTA TCTGGAGCTC 
GATCACTGCA TCCAGCCTGC TATCATCACC AAGGACATCT GTATGGTCTT CTACTCCAGA 
GATGCCAAGA TCTCGCCTCC TCGCTCGCTG AGAAACTTGT TTGGCAGCGG CTACTCCAAA 
ACCCCGGACT GTAATAAAGT GACTGGAGTC TATGAGCTGA GCTTGTGCAA GATAGCAGAC 
ACCGGAAGCC CAGGTATGCA GCGGCGCAGA CGGAAGGTTC TGGACACGTC AGTGGCATAT 
GTGCGTGGAG AGGAGAATCT GGCCGGCTGG AGACCCCGAG GAGACAGTCT GATCCTGGAG 
CACCAGTGGG AGCTGGACAA AATGGAGCAG CTGCATGAGG TGGAAAAGAC GCGTCACTTG 
CTTCTGCTGC GTGAGAAGCT GGGAGAAACA CCTCCACCTA AATCTCTGAG CGAGTCTCTG 
TCCCCCAGCC TGAGCAGCGG GACCCTCAGC ACCTCCACCA GCATCTCCTC CCAGATCTCC 
TCCACAACTT TCGAGAGTGC CATCACCCCC AGCGAAAGCA GCGGCTATGA CTCCACGGAC 
ATCGAGAGCT TGGTGGATCG AGAGAAGGAG CTGGCCACTA AGTGCCTCCG TCTCCTGACA 
CACACTTTCA ACAGTGAATA CAACCAAATG TGCAACAGCA TTAGTGACTG CAAGTTTTCA 
GATATCTCTC CTATGGGTCG GGATCCATCA GTCAGTAGTT TCAGCAGTGC CACCCTCACA 
CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC TCCAGGACCC CTGAAGCAAA CTCTCGTGCA 
ACCAGCCCCT CAGGCTCAGA CTATGAGAAC TTCCCCATGG TGCCCATTCT GGAGACGTCC 
TACCTGGCCC GCGCCGGCAA ACACGAGTTC CTCAACCTTG TGCCTAACAT AGAGGAAATG 
AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC ATCTTGAATT TCATGGAGCC CCGCTCGAAC 
ACCTGGGTGA AGCACTTTGT GGTGGTGCGT CGGCCGTATG TGTTCATCTA CAACAATGAC 
AAGGACCCTG TGGAGAGAGG AGTGCTGAAC TTATCCACTG CACAGGTGGA GTACAGTGAA 
GACCAGCAGG CCATGCTCAA GACGCCTCAT ACATTTGCCA TGTGCACCAA ACACAGAGGA 
ATCCTGCTTC AGGCCAACAA TGACAAAGAC ATGAACGACT GGCTGTATGC CTTTAACCCA 
CTGCTAGCAG GAACAATAAG GTCGAAGCTT GCAAGGAGAC GGTCTGGTGT GATGAAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296822.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295097.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.

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CloneID	ODa40270
Clone ID Related Accession (Same CDS sequence)	XM_009296822.2 , XM_009296822.3
Accession Version	XM_009296822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GAGCCTTCGT GTACCTGAGT 
AACCTGCTGT ACCCTGTTCC TCTGGTGCAT CGAGTCGCTG TAGTGACAGA GAAAGGAGAT 
GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG GCCATCGCCG CGGATGAAGA GGCACCTGAT 
TATGGGTCTG GTGTGCGTCA GTCAGGAACT GCTAAGATTT CCTTTGATAA TGAGTATTTT 
AAAAAGAATG ACTTCACCTC AGTGGCCATG ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG 
CGCATTGTAG AAGGTCAAGG TCAGAGTTCA GAGGTCATTA CGCCTTCAGA GGAGATGAAC 
AGAATCAATG AAATGGACCT AAAGCTGGGG AACGTGGACA CAAAACTGGG AGACGGTTTG 
GCCGAGCATC TGGAAGTGGG CAGCATTTTC ACTTTCCGCG TCACCGTTCT GCAGGCAAGC 
GGCATCCCGG CCGAATACGC AGACATCTTC TGCCAGTTCA ATTTCCTCCA CCGTCACGAT 
GAGGCGTTCA GCACTGAGCC TCTGAAGAAC ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC 
CACGTACAGA ATATCTCAGT GGAGGTGACA GAGTCCTTCA TTGAGTACAT AAAGACCAAG 
CCAATTGTAT TTGAAGTGTT TGGCCATTAC CAGCAACACC CACTGCACAT TCATGGACAG 
GATGTGGTCA GCCCTTCTCA GCCTTCCAGA AAGTACTATC CTCCACCAAT GCCTCTGTCT 
AAACCAGATC ACGCTCGTAA GATTGAGCTT ATCCGTTACC TGATGAGCGG GTATGTTCAT 
GGTCAGGTGT TGGACACATT GTCAGAGCAT GCCAACGCTT TGGCCTCCAC CGCCGTGGCC 
AGTCTGGAGG AAGGGGCATA TGAGCTGTGC CACTTCCCCT TCCTCCCCAT ACCCAACTGC 
CCCGTCTTCA GAGTGCCCCG ACACGACGTG CCAGCCACCA AACTGAACAC CATCACCAAG 
TCCAACCTGG GTCAGTGTGT GAGCAAGTAC GACTTGCTGG CCTGGTTTGA GATCAGCGAA 
CTGGAGCCCA CTGGAGAGTA TATTCCAGCA GTAGTGGATC ATACAGGAGG TCTTCCCTGT 
CATGGCACAT ACCTGCTGCA CCAGGGCATC CAGCGCAGAA TAACAGTGAC TCTGATCCAT 
GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC GTCCGAGAGC TGGTGGTCGG TCGAATCAGG 
AATAAGCCAG AAGTGGATGA TTCAGCCGCA GACGCCGTCT TGTCTCTCAA CATCATCTCA 
GCTAAAAACC TCAAATCCTC CCACAACTCC AGCAGCCTTT GTATTGATAG CGATATTTCT 
AGGACGTTCT ACCGGTTTGA GGCCGTGTGG GACAGTTCTC TGCACAACTC TCTCCTACTG 
AACCGCGTCA CTCCATACGG AGAGAAGATC TACATGACCC TGTCTGCCTA TCTGGAGCTC 
GATCACTGCA TCCAGCCTGC TATCATCACC AAGGACATCT GTATGGTCTT CTACTCCAGA 
GATGCCAAGA TCTCGCCTCC TCGCTCGCTG AGAAACTTGT TTGGCAGCGG CTACTCCAAA 
ACCCCGGACT GTAATAAAGT GACTGGAGTC TATGAGCTGA GCTTGTGCAA GATAGCAGAC 
ACCGGAAGCC CAGGTATGCA GCGGCGCAGA CGGAAGGTTC TGGACACGTC AGTGGCATAT 
GTGCGTGGAG AGGAGAATCT GGCCGGCTGG AGACCCCGAG GAGACAGTCT GATCCTGGAG 
CACCAGTGGG AGCTGGACAA AATGGAGCAG CTGCATGAGG TGGAAAAGAC GCGTCACTTG 
CTTCTGCTGC GTGAGAAGCT GGGAGAAACA CCTCCACCTA AATCTCTGAG CGAGTCTCTG 
TCCCCCAGCC TGAGCAGCGG GACCCTCAGC ACCTCCACCA GCATCTCCTC CCAGATCTCC 
TCCACAACTT TCGAGAGTGC CATCACCCCC AGCGAAAGCA GCGGCTATGA CTCCACGGAC 
ATCGAGAGCT TGGTGGATCG AGAGAAGGAG CTGGCCACTA AGTGCCTCCG TCTCCTGACA 
CACACTTTCA ACAGTGAATA CAACCAAATG TGCAACAGCA TTAGTGACTG CAAGTTTTCA 
GATATCTCTC CTATGGGTCG GGATCCATCA GTCAGTAGTT TCAGCAGTGC CACCCTCACA 
CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC TCCAGGACCC CTGAAGCAAA CTCTCGTGCA 
ACCAGCCCCT CAGGCTCAGA CTATGAGAAC TTCCCCATGG TGCCCATTCT GGAGACGTCC 
TACCTGGCCC GCGCCGGCAA ACACGAGTTC CTCAACCTTG TGCCTAACAT AGAGGAAATG 
AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC ATCTTGAATT TCATGGAGCC CCGCTCGAAC 
ACCTGGGTGA AGCACTTTGT GGTGGTGCGT CGGCCGTATG TGTTCATCTA CAACAATGAC 
AAGGACCCTG TGGAGAGAGG AGTGCTGAAC TTATCCACTG CACAGGTGGA GTACAGTGAA 
GACCAGCAGG CCATGCTCAA GACGCCTCAT ACATTTGCCA TGTGCACCAA ACACAGAGGA 
ATCCTGCTTC AGGCCAACAA TGACAAAGAC ATGAACGACT GGCTGTATGC CTTTAACCCA 
CTGCTAGCAG GAACAATAAG GTCGAAGCTT GCAAGGAGAC GGTCTGGTGT GATGAAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295097.1
CDS	320..5842
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296822.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296822.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATG TGACGATATT AACGATGTTA AAACGCTGCT AAGTTCACAA 
ACTGAAGCAA TGCTCTATCG GAGAAAAGCA CCCATGGGCT GTCTGACAGC CTCTCCATCT 
TCAGGCTCTC TCTGCAGCCA GGCAGGCCTA CAGTCCGTCT CCAGCATCCA GGAGCGCATC 
ATGTCCACGC CAGGCGGAGA AGAGGCCATC GAGAGGCTCA AGGAATCAGA AAAGATCATA 
GCCGAGCTGA ATGAGACCTG GGAAGAGAAG CTGAGGAAGA CCGAGGCCAT CCGTATGGAG 
AGAGAGGCGC TGCTGGCAGA GATGGGAGTT GCCATACGGG AGGACGGGGG AACCCTGGGC 
GTCTTCTCTC CTAAAAAGTT TGGTCCTGAT AGACCAACGT CACTGTCCAT TGAGGACTTT 
AGTGTTTATT CTTCCAATCA GCTTTTCGTT GAAAGGCCCT GTGAAGTCTA TACTGACTTT 
AATGGGGTGT TTTCACCCAA AAAGACGCCT CACCTGGTGA ACCTGAACGA GGATCCTTTG 
ATGTCAGAAT GTCTGCTCTA TTATATCAAA GATGGAATAA CAAGGGTGGG CCAGGCAGAT 
GCTGAAAGAA GACAGGATAT CGTTCTAAGT GGAGCTCACA TTAAAGAGGA GCACTGTATT 
TTCCGCAGTG AGAGGAATGC CAATGGACAT GTGATTGTCA CGTTGGAGCC ATGTGAGGGA 
TCAGAAACAT ATGTAAATGG TAAACGTGTG AATTCTGCGG TCCAGCTGCG CTCAGGTAAC 
CGCATTATCA TGGGAAAGAA CCACGTGTTC CGGTTTAACC ACCCTGAGCA GGCTCGCGCT 
GAGCGTGAGA AGACCCCGTC AGCAGAGACA CCTGTAGAAC CTGTTGACTG GACTTTTGCC 
CAAAGAGAAC TGCTGGAGAA ACAGGGTATC GACATGAAGC AAGAGATGGA GAAGAGGCTT 
ACAGAAATGG AGATCCTGTA CAAAAAAGAG AAGGAGGAAG CGGACCAGCT ACTGGAGCAA 
CAGAGACTGG TGTATGAGAG CAAACTGCAA GAGCTGCAGA AACAAGTGGA GACTCGTTCT 
TTGGCTGCAG AGACTCCAGA TGAGGAGGAA GAAGAAGAGG AGGAGGATGA AGTGCCCTGG 
ACCCAGCATA AATATGAGCT GGCTCAGTGG GCTTTCAGGA AGTGGAGATA CCACCAGTTC 
ACCTCCCTCA GAGACCAATT ATGGGGCAAT GCTGTGTACC TCAAAGAAGC CAATGCCATC 
AGTGTGGAGC TCAAGAAAAA GGTCCAGTTC CAGTTCGTCT TGCTCACAGA CACCCTCTAT 
TCTCCACTGC CCCCGGAGCT GCTGCCTCCA GAGCCAGAGA AAGAGCGAGA CGCCAGGCCT 
TTTCCGAGAA CAGTGGTGGC TGTGGAGGTT CAGGACCTGA AGAATGGAGC CACTCACTAC 
TGGTCCCTGG AGAAGCTGAA GCAGAGGCTG GACCAGATGC GAGAGATGTA CGACCGTGCT 
GGCGAAATGG CCTCGTCCAA TCAGGGAGAT GATGGGGAGG GTGCGTTGAC CGGCAGTGAC 
CCGTTCTATG ACCGATTTCA TTGGTTTAAG CTGGTTGGGA GAGCCTTCGT GTACCTGAGT 
AACCTGCTGT ACCCTGTTCC TCTGGTGCAT CGAGTCGCTG TAGTGACAGA GAAAGGAGAT 
GTGCGAGGAT TTCTGCGTGT GGGGGTCCAG GCCATCGCCG CGGATGAAGA GGCACCTGAT 
TATGGGTCTG GTGTGCGTCA GTCAGGAACT GCTAAGATTT CCTTTGATAA TGAGTATTTT 
AAAAAGAATG ACTTCACCTC AGTGGCCATG ACCCGCTCTG GTCTCTCTCT GGAGGAGCTG 
CGCATTGTAG AAGGTCAAGG TCAGAGTTCA GAGGTCATTA CGCCTTCAGA GGAGATGAAC 
AGAATCAATG AAATGGACCT AAAGCTGGGG AACGTGGACA CAAAACTGGG AGACGGTTTG 
GCCGAGCATC TGGAAGTGGG CAGCATTTTC ACTTTCCGCG TCACCGTTCT GCAGGCAAGC 
GGCATCCCGG CCGAATACGC AGACATCTTC TGCCAGTTCA ATTTCCTCCA CCGTCACGAT 
GAGGCGTTCA GCACTGAGCC TCTGAAGAAC ACTGGCAGAG GAGCCCCGCT GGGCTTTTAC 
CACGTACAGA ATATCTCAGT GGAGGTGACA GAGTCCTTCA TTGAGTACAT AAAGACCAAG 
CCAATTGTAT TTGAAGTGTT TGGCCATTAC CAGCAACACC CACTGCACAT TCATGGACAG 
GATGTGGTCA GCCCTTCTCA GCCTTCCAGA AAGTACTATC CTCCACCAAT GCCTCTGTCT 
AAACCAGATC ACGCTCGTAA GATTGAGCTT ATCCGTTACC TGATGAGCGG GTATGTTCAT 
GGTCAGGTGT TGGACACATT GTCAGAGCAT GCCAACGCTT TGGCCTCCAC CGCCGTGGCC 
AGTCTGGAGG AAGGGGCATA TGAGCTGTGC CACTTCCCCT TCCTCCCCAT ACCCAACTGC 
CCCGTCTTCA GAGTGCCCCG ACACGACGTG CCAGCCACCA AACTGAACAC CATCACCAAG 
TCCAACCTGG GTCAGTGTGT GAGCAAGTAC GACTTGCTGG CCTGGTTTGA GATCAGCGAA 
CTGGAGCCCA CTGGAGAGTA TATTCCAGCA GTAGTGGATC ATACAGGAGG TCTTCCCTGT 
CATGGCACAT ACCTGCTGCA CCAGGGCATC CAGCGCAGAA TAACAGTGAC TCTGATCCAT 
GAGAAGGGAA GTGAGCTGCA CTGGAAGGAC GTCCGAGAGC TGGTGGTCGG TCGAATCAGG 
AATAAGCCAG AAGTGGATGA TTCAGCCGCA GACGCCGTCT TGTCTCTCAA CATCATCTCA 
GCTAAAAACC TCAAATCCTC CCACAACTCC AGCAGCCTTT GTATTGATAG CGATATTTCT 
AGGACGTTCT ACCGGTTTGA GGCCGTGTGG GACAGTTCTC TGCACAACTC TCTCCTACTG 
AACCGCGTCA CTCCATACGG AGAGAAGATC TACATGACCC TGTCTGCCTA TCTGGAGCTC 
GATCACTGCA TCCAGCCTGC TATCATCACC AAGGACATCT GTATGGTCTT CTACTCCAGA 
GATGCCAAGA TCTCGCCTCC TCGCTCGCTG AGAAACTTGT TTGGCAGCGG CTACTCCAAA 
ACCCCGGACT GTAATAAAGT GACTGGAGTC TATGAGCTGA GCTTGTGCAA GATAGCAGAC 
ACCGGAAGCC CAGGTATGCA GCGGCGCAGA CGGAAGGTTC TGGACACGTC AGTGGCATAT 
GTGCGTGGAG AGGAGAATCT GGCCGGCTGG AGACCCCGAG GAGACAGTCT GATCCTGGAG 
CACCAGTGGG AGCTGGACAA AATGGAGCAG CTGCATGAGG TGGAAAAGAC GCGTCACTTG 
CTTCTGCTGC GTGAGAAGCT GGGAGAAACA CCTCCACCTA AATCTCTGAG CGAGTCTCTG 
TCCCCCAGCC TGAGCAGCGG GACCCTCAGC ACCTCCACCA GCATCTCCTC CCAGATCTCC 
TCCACAACTT TCGAGAGTGC CATCACCCCC AGCGAAAGCA GCGGCTATGA CTCCACGGAC 
ATCGAGAGCT TGGTGGATCG AGAGAAGGAG CTGGCCACTA AGTGCCTCCG TCTCCTGACA 
CACACTTTCA ACAGTGAATA CAACCAAATG TGCAACAGCA TTAGTGACTG CAAGTTTTCA 
GATATCTCTC CTATGGGTCG GGATCCATCA GTCAGTAGTT TCAGCAGTGC CACCCTCACA 
CCCTCCTCCA CCTGCCCCTC GCTCTCAGAC TCCAGGACCC CTGAAGCAAA CTCTCGTGCA 
ACCAGCCCCT CAGGCTCAGA CTATGAGAAC TTCCCCATGG TGCCCATTCT GGAGACGTCC 
TACCTGGCCC GCGCCGGCAA ACACGAGTTC CTCAACCTTG TGCCTAACAT AGAGGAAATG 
AGGCCTGGGT CTGTGGTGTC CAAGAAGGGC ATCTTGAATT TCATGGAGCC CCGCTCGAAC 
ACCTGGGTGA AGCACTTTGT GGTGGTGCGT CGGCCGTATG TGTTCATCTA CAACAATGAC 
AAGGACCCTG TGGAGAGAGG AGTGCTGAAC TTATCCACTG CACAGGTGGA GTACAGTGAA 
GACCAGCAGG CCATGCTCAA GACGCCTCAT ACATTTGCCA TGTGCACCAA ACACAGAGGA 
ATCCTGCTTC AGGCCAACAA TGACAAAGAC ATGAACGACT GGCTGTATGC CTTTAACCCA 
CTGCTAGCAG GAACAATAAG GTCGAAGCTT GCAAGGAGAC GGTCTGGTGT GATGAAGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296823.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295098.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.

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CloneID	ODa40271
Clone ID Related Accession (Same CDS sequence)	XM_009296823.2 , XM_009296823.3
Accession Version	XM_009296823.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATA CCTGAAAGAT ATACACAACA ATAAGCATAG ATACTTGCTT 
GCCTCAGAGA ACCAACGGCC TGGCCATTTC TCCACAGCAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295098.1
CDS	319..5748
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKYLKDIHNNKHRYLLASENQRPGHFSTAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296823.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296823.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATA CCTGAAAGAT ATACACAACA ATAAGCATAG ATACTTGCTT 
GCCTCAGAGA ACCAACGGCC TGGCCATTTC TCCACAGCAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296823.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295098.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.

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CloneID	ODa40271
Clone ID Related Accession (Same CDS sequence)	XM_009296823.2 , XM_009296823.3
Accession Version	XM_009296823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATA CCTGAAAGAT ATACACAACA ATAAGCATAG ATACTTGCTT 
GCCTCAGAGA ACCAACGGCC TGGCCATTTC TCCACAGCAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295098.1
CDS	320..5749
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKYLKDIHNNKHRYLLASENQRPGHFSTAPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296823.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296823.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATA CCTGAAAGAT ATACACAACA ATAAGCATAG ATACTTGCTT 
GCCTCAGAGA ACCAACGGCC TGGCCATTTC TCCACAGCAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296824.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295099.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.

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CloneID	ODa40272
Clone ID Related Accession (Same CDS sequence)	XM_009296824.2 , XM_009296824.3
Accession Version	XM_009296824.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295099.1
CDS	319..5670
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296824.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296824.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296824.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295099.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.

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CloneID	ODa40272
Clone ID Related Accession (Same CDS sequence)	XM_009296824.2 , XM_009296824.3
Accession Version	XM_009296824.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295099.1
CDS	320..5671
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDIEPMGDDCLGSGSKSPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296824.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296824.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACATCG AGCCAATGGG GGATGATTGC 
CTTGGAAGTG GAAGCAAATC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296825.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295100.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.

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CloneID	ODa40273
Clone ID Related Accession (Same CDS sequence)	XM_009296825.3 , XM_009296825.2
Accession Version	XM_009296825.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295100.1
CDS	319..5628
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296825.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296825.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296825.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295100.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.

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CloneID	ODa40273
Clone ID Related Accession (Same CDS sequence)	XM_009296825.3 , XM_009296825.2
Accession Version	XM_009296825.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295100.1
CDS	319..5628
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296825.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296825.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGCTCACC TATTTTTCAT 
GGCTGTGTGA ACGAGCGTCT GGCCGACCGC ACACCATCGC CCACCTTCTC CACCACCGAT 
TCGGAGATCA CTGAGCTGGC CGACGAGCGA CAGAGCGAGA TGGAGGACTT TATGGATGAC 
GAGGCTTTCG TGGATGACAC CAGCTCAGAT GCTGGAACCG AGGAGGGCTC GGACATCTTC 
AGTGATGGCC AGGACCCCTT CTATGATCGT TCTCCATGGT TCATCCTGGT GGGCAGAGCC 
TTCGTGTACC TGAGTAACCT GCTGTACCCT GTTCCTCTGG TGCATCGAGT CGCTGTAGTG 
ACAGAGAAAG GAGATGTGCG AGGATTTCTG CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT 
GAAGAGGCAC CTGATTATGG GTCTGGTGTG CGTCAGTCAG GAACTGCTAA GATTTCCTTT 
GATAATGAGT ATTTTAAAAA GAATGACTTC ACCTCAGTGG CCATGACCCG CTCTGGTCTC 
TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT CAAGGTCAGA GTTCAGAGGT CATTACGCCT 
TCAGAGGAGA TGAACAGAAT CAATGAAATG GACCTAAAGC TGGGGAACGT GGACACAAAA 
CTGGGAGACG GTTTGGCCGA GCATCTGGAA GTGGGCAGCA TTTTCACTTT CCGCGTCACC 
GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA TACGCAGACA TCTTCTGCCA GTTCAATTTC 
CTCCACCGTC ACGATGAGGC GTTCAGCACT GAGCCTCTGA AGAACACTGG CAGAGGAGCC 
CCGCTGGGCT TTTACCACGT ACAGAATATC TCAGTGGAGG TGACAGAGTC CTTCATTGAG 
TACATAAAGA CCAAGCCAAT TGTATTTGAA GTGTTTGGCC ATTACCAGCA ACACCCACTG 
CACATTCATG GACAGGATGT GGTCAGCCCT TCTCAGCCTT CCAGAAAGTA CTATCCTCCA 
CCAATGCCTC TGTCTAAACC AGTGCCAGCC ACCAAACTGA ACACCATCAC CAAGTCCAAC 
CTGGGTCAGT GTGTGAGCAA GTACGACTTG CTGGCCTGGT TTGAGATCAG CGAACTGGAG 
CCCACTGGAG AGTATATTCC AGCAGTAGTG GATCATACAG GAGGTCTTCC CTGTCATGGC 
ACATACCTGC TGCACCAGGG CATCCAGCGC AGAATAACAG TGACTCTGAT CCATGAGAAG 
GGAAGTGAGC TGCACTGGAA GGACGTCCGA GAGCTGGTGG TCGGTCGAAT CAGGAATAAG 
CCAGAAGTGG ATGATTCAGC CGCAGACGCC GTCTTGTCTC TCAACATCAT CTCAGCTAAA 
AACCTCAAAT CCTCCCACAA CTCCAGCAGG ACGTTCTACC GGTTTGAGGC CGTGTGGGAC 
AGTTCTCTGC ACAACTCTCT CCTACTGAAC CGCGTCACTC CATACGGAGA GAAGATCTAC 
ATGACCCTGT CTGCCTATCT GGAGCTCGAT CACTGCATCC AGCCTGCTAT CATCACCAAG 
GACATCTGTA TGGTCTTCTA CTCCAGAGAT GCCAAGATCT CGCCTCCTCG CTCGCTGAGA 
AACTTGTTTG GCAGCGGCTA CTCCAAAACC CCGGACTGTA ATAAAGTGAC TGGAGTCTAT 
GAGCTGAGCT TGTGCAAGAT AGCAGACACC GGAAGCCCAG GTATGCAGCG GCGCAGACGG 
AAGGTTCTGG ACACGTCAGT GGCATATGTG CGTGGAGAGG AGAATCTGGC CGGCTGGAGA 
CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC CAGTGGGAGC TGGACAAAAT GGAGCAGCTG 
CATGAGGTGG AAAAGACGCG TCACTTGCTT CTGCTGCGTG AGAAGCTGGG AGAAACACCT 
CCACCTAAAT CTCTGAGCGA GTCTCTGTCC CCCAGCCTGA GCAGCGGGAC CCTCAGCACC 
TCCACCAGCA TCTCCTCCCA GATCTCCTCC ACAACTTTCG AGAGTGCCAT CACCCCCAGC 
GAAAGCAGCG GCTATGACTC CACGGACATC GAGAGCTTGG TGGATCGAGA GAAGGAGCTG 
GCCACTAAGT GCCTCCGTCT CCTGACACAC ACTTTCAACA GTGAATACAA CCAAATGTGC 
AACAGCATTA GTGACTGCAA GTTTTCAGAT ATCTCTCCTA TGGGTCGGGA TCCATCAGTC 
AGTAGTTTCA GCAGTGCCAC CCTCACACCC TCCTCCACCT GCCCCTCGCT CTCAGACTCC 
AGGACCCCTG AAGCAAACTC TCGTGCAACC AGCCCCTCAG GCTCAGACTA TGAGAACTTC 
CCCATGGTGC CCATTCTGGA GACGTCCTAC CTGGCCCGCG CCGGCAAACA CGAGTTCCTC 
AACCTTGTGC CTAACATAGA GGAAATGAGG CCTGGGTCTG TGGTGTCCAA GAAGGGCATC 
TTGAATTTCA TGGAGCCCCG CTCGAACACC TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG 
CCGTATGTGT TCATCTACAA CAATGACAAG GACCCTGTGG AGAGAGGAGT GCTGAACTTA 
TCCACTGCAC AGGTGGAGTA CAGTGAAGAC CAGCAGGCCA TGCTCAAGAC GCCTCATACA 
TTTGCCATGT GCACCAAACA CAGAGGAATC CTGCTTCAGG CCAACAATGA CAAAGACATG 
AACGACTGGC TGTATGCCTT TAACCCACTG CTAGCAGGAA CAATAAGGTC GAAGCTTGCA 
AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296826.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295101.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.

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CloneID	ODa40274
Clone ID Related Accession (Same CDS sequence)	XM_009296826.2 , XM_009296826.3
Accession Version	XM_009296826.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295101.1
CDS	319..5610
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296826.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296826.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296826.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295101.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.

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CloneID	ODa40274
Clone ID Related Accession (Same CDS sequence)	XM_009296826.2 , XM_009296826.3
Accession Version	XM_009296826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296826.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295101.1
CDS	319..5610
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTTDSEITELADERQSEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFILVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296826.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296826.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGCTCA CCTATTTTTC ATGGCTGTGT GAACGAGCGT 
CTGGCCGACC GCACACCATC GCCCACCTTC TCCACCACCG ATTCGGAGAT CACTGAGCTG 
GCCGACGAGC GACAGAGCGA GATGGAGGAC TTTATGGATG ACGAGGCTTT CGTGGATGAC 
ACCAGCTCAG ATGCTGGAAC CGAGGAGGGC TCGGACATCT TCAGTGATGG CCAGGACCCC 
TTCTATGATC GTTCTCCATG GTTCATCCTG GTGGGCAGAG CCTTCGTGTA CCTGAGTAAC 
CTGCTGTACC CTGTTCCTCT GGTGCATCGA GTCGCTGTAG TGACAGAGAA AGGAGATGTG 
CGAGGATTTC TGCGTGTGGG GGTCCAGGCC ATCGCCGCGG ATGAAGAGGC ACCTGATTAT 
GGGTCTGGTG TGCGTCAGTC AGGAACTGCT AAGATTTCCT TTGATAATGA GTATTTTAAA 
AAGAATGACT TCACCTCAGT GGCCATGACC CGCTCTGGTC TCTCTCTGGA GGAGCTGCGC 
ATTGTAGAAG GTCAAGGTCA GAGTTCAGAG GTCATTACGC CTTCAGAGGA GATGAACAGA 
ATCAATGAAA TGGACCTAAA GCTGGGGAAC GTGGACACAA AACTGGGAGA CGGTTTGGCC 
GAGCATCTGG AAGTGGGCAG CATTTTCACT TTCCGCGTCA CCGTTCTGCA GGCAAGCGGC 
ATCCCGGCCG AATACGCAGA CATCTTCTGC CAGTTCAATT TCCTCCACCG TCACGATGAG 
GCGTTCAGCA CTGAGCCTCT GAAGAACACT GGCAGAGGAG CCCCGCTGGG CTTTTACCAC 
GTACAGAATA TCTCAGTGGA GGTGACAGAG TCCTTCATTG AGTACATAAA GACCAAGCCA 
ATTGTATTTG AAGTGTTTGG CCATTACCAG CAACACCCAC TGCACATTCA TGGACAGGAT 
GTGGTCAGCC CTTCTCAGCC TTCCAGAAAG TACTATCCTC CACCAATGCC TCTGTCTAAA 
CCAGTGCCAG CCACCAAACT GAACACCATC ACCAAGTCCA ACCTGGGTCA GTGTGTGAGC 
AAGTACGACT TGCTGGCCTG GTTTGAGATC AGCGAACTGG AGCCCACTGG AGAGTATATT 
CCAGCAGTAG TGGATCATAC AGGAGGTCTT CCCTGTCATG GCACATACCT GCTGCACCAG 
GGCATCCAGC GCAGAATAAC AGTGACTCTG ATCCATGAGA AGGGAAGTGA GCTGCACTGG 
AAGGACGTCC GAGAGCTGGT GGTCGGTCGA ATCAGGAATA AGCCAGAAGT GGATGATTCA 
GCCGCAGACG CCGTCTTGTC TCTCAACATC ATCTCAGCTA AAAACCTCAA ATCCTCCCAC 
AACTCCAGCA GGACGTTCTA CCGGTTTGAG GCCGTGTGGG ACAGTTCTCT GCACAACTCT 
CTCCTACTGA ACCGCGTCAC TCCATACGGA GAGAAGATCT ACATGACCCT GTCTGCCTAT 
CTGGAGCTCG ATCACTGCAT CCAGCCTGCT ATCATCACCA AGGACATCTG TATGGTCTTC 
TACTCCAGAG ATGCCAAGAT CTCGCCTCCT CGCTCGCTGA GAAACTTGTT TGGCAGCGGC 
TACTCCAAAA CCCCGGACTG TAATAAAGTG ACTGGAGTCT ATGAGCTGAG CTTGTGCAAG 
ATAGCAGACA CCGGAAGCCC AGGTATGCAG CGGCGCAGAC GGAAGGTTCT GGACACGTCA 
GTGGCATATG TGCGTGGAGA GGAGAATCTG GCCGGCTGGA GACCCCGAGG AGACAGTCTG 
ATCCTGGAGC ACCAGTGGGA GCTGGACAAA ATGGAGCAGC TGCATGAGGT GGAAAAGACG 
CGTCACTTGC TTCTGCTGCG TGAGAAGCTG GGAGAAACAC CTCCACCTAA ATCTCTGAGC 
GAGTCTCTGT CCCCCAGCCT GAGCAGCGGG ACCCTCAGCA CCTCCACCAG CATCTCCTCC 
CAGATCTCCT CCACAACTTT CGAGAGTGCC ATCACCCCCA GCGAAAGCAG CGGCTATGAC 
TCCACGGACA TCGAGAGCTT GGTGGATCGA GAGAAGGAGC TGGCCACTAA GTGCCTCCGT 
CTCCTGACAC ACACTTTCAA CAGTGAATAC AACCAAATGT GCAACAGCAT TAGTGACTGC 
AAGTTTTCAG ATATCTCTCC TATGGGTCGG GATCCATCAG TCAGTAGTTT CAGCAGTGCC 
ACCCTCACAC CCTCCTCCAC CTGCCCCTCG CTCTCAGACT CCAGGACCCC TGAAGCAAAC 
TCTCGTGCAA CCAGCCCCTC AGGCTCAGAC TATGAGAACT TCCCCATGGT GCCCATTCTG 
GAGACGTCCT ACCTGGCCCG CGCCGGCAAA CACGAGTTCC TCAACCTTGT GCCTAACATA 
GAGGAAATGA GGCCTGGGTC TGTGGTGTCC AAGAAGGGCA TCTTGAATTT CATGGAGCCC 
CGCTCGAACA CCTGGGTGAA GCACTTTGTG GTGGTGCGTC GGCCGTATGT GTTCATCTAC 
AACAATGACA AGGACCCTGT GGAGAGAGGA GTGCTGAACT TATCCACTGC ACAGGTGGAG 
TACAGTGAAG ACCAGCAGGC CATGCTCAAG ACGCCTCATA CATTTGCCAT GTGCACCAAA 
CACAGAGGAA TCCTGCTTCA GGCCAACAAT GACAAAGACA TGAACGACTG GCTGTATGCC 
TTTAACCCAC TGCTAGCAGG AACAATAAGG TCGAAGCTTG CAAGGAGACG GTCTGGTGTG 
ATGAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296827.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295102.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.

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CloneID	ODa40275
Clone ID Related Accession (Same CDS sequence)	XM_009296827.2 , XM_009296827.3
Accession Version	XM_009296827.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295102.1
CDS	319..5604
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296827.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296827.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296827.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295102.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.

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CloneID	ODa40275
Clone ID Related Accession (Same CDS sequence)	XM_009296827.2 , XM_009296827.3
Accession Version	XM_009296827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295102.1
CDS	320..5605
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCPVFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296827.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296827.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG ATCACGCTCG TAAGATTGAG CTTATCCGTT ACCTGATGAG CGGGTATGTT 
CATGGTCAGG TGTTGGACAC ATTGTCAGAG CATGCCAACG CTTTGGCCTC CACCGCCGTG 
GCCAGTCTGG AGGAAGGGGC ATATGAGCTG TGCCACTTCC CCTTCCTCCC CATACCCAAC 
TGCCCCGTCT TCAGAGTGCC CCGACACGAC GTGCCAGCCA CCAAACTGAA CACCATCACC 
AAGTCCAACC TGGGTCAGTG TGTGAGCAAG TACGACTTGC TGGCCTGGTT TGAGATCAGC 
GAACTGGAGC CCACTGGAGA GTATATTCCA GCAGTAGTGG ATCATACAGG AGGTCTTCCC 
TGTCATGGCA CATACCTGCT GCACCAGGGC ATCCAGCGCA GAATAACAGT GACTCTGATC 
CATGAGAAGG GAAGTGAGCT GCACTGGAAG GACGTCCGAG AGCTGGTGGT CGGTCGAATC 
AGGAATAAGC CAGAAGTGGA TGATTCAGCC GCAGACGCCG TCTTGTCTCT CAACATCATC 
TCAGCTAAAA ACCTCAAATC CTCCCACAAC TCCAGCAGCC TTTGTATTGA TAGCGATATT 
TCTAGGACGT TCTACCGGTT TGAGGCCGTG TGGGACAGTT CTCTGCACAA CTCTCTCCTA 
CTGAACCGCG TCACTCCATA CGGAGAGAAG ATCTACATGA CCCTGTCTGC CTATCTGGAG 
CTCGATCACT GCATCCAGCC TGCTATCATC ACCAAGGACA TCTGTATGGT CTTCTACTCC 
AGAGATGCCA AGATCTCGCC TCCTCGCTCG CTGAGAAACT TGTTTGGCAG CGGCTACTCC 
AAAACCCCGG ACTGTAATAA AGTGACTGGA GTCTATGAGC TGAGCTTGTG CAAGATAGCA 
GACACCGGAA GCCCAGGTAT GCAGCGGCGC AGACGGAAGG TTCTGGACAC GTCAGTGGCA 
TATGTGCGTG GAGAGGAGAA TCTGGCCGGC TGGAGACCCC GAGGAGACAG TCTGATCCTG 
GAGCACCAGT GGGAGCTGGA CAAAATGGAG CAGCTGCATG AGGTGGAAAA GACGCGTCAC 
TTGCTTCTGC TGCGTGAGAA GCTGGGAGAA ACACCTCCAC CTAAATCTCT GAGCGAGTCT 
CTGTCCCCCA GCCTGAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCCCAGATC 
TCCTCCACAA CTTTCGAGAG TGCCATCACC CCCAGCGAAA GCAGCGGCTA TGACTCCACG 
GACATCGAGA GCTTGGTGGA TCGAGAGAAG GAGCTGGCCA CTAAGTGCCT CCGTCTCCTG 
ACACACACTT TCAACAGTGA ATACAACCAA ATGTGCAACA GCATTAGTGA CTGCAAGTTT 
TCAGATATCT CTCCTATGGG TCGGGATCCA TCAGTCAGTA GTTTCAGCAG TGCCACCCTC 
ACACCCTCCT CCACCTGCCC CTCGCTCTCA GACTCCAGGA CCCCTGAAGC AAACTCTCGT 
GCAACCAGCC CCTCAGGCTC AGACTATGAG AACTTCCCCA TGGTGCCCAT TCTGGAGACG 
TCCTACCTGG CCCGCGCCGG CAAACACGAG TTCCTCAACC TTGTGCCTAA CATAGAGGAA 
ATGAGGCCTG GGTCTGTGGT GTCCAAGAAG GGCATCTTGA ATTTCATGGA GCCCCGCTCG 
AACACCTGGG TGAAGCACTT TGTGGTGGTG CGTCGGCCGT ATGTGTTCAT CTACAACAAT 
GACAAGGACC CTGTGGAGAG AGGAGTGCTG AACTTATCCA CTGCACAGGT GGAGTACAGT 
GAAGACCAGC AGGCCATGCT CAAGACGCCT CATACATTTG CCATGTGCAC CAAACACAGA 
GGAATCCTGC TTCAGGCCAA CAATGACAAA GACATGAACG ACTGGCTGTA TGCCTTTAAC 
CCACTGCTAG CAGGAACAAT AAGGTCGAAG CTTGCAAGGA GACGGTCTGG TGTGATGAAG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296828.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295103.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.

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CloneID	ODa40276
Clone ID Related Accession (Same CDS sequence)	XM_009296828.3 , XM_009296828.2
Accession Version	XM_009296828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGAGCC TTCGTGTACC TGAGTAACCT GCTGTACCCT 
GTTCCTCTGG TGCATCGAGT CGCTGTAGTG ACAGAGAAAG GAGATGTGCG AGGATTTCTG 
CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT GAAGAGGCAC CTGATTATGG GTCTGGTGTG 
CGTCAGTCAG GAACTGCTAA GATTTCCTTT GATAATGAGT ATTTTAAAAA GAATGACTTC 
ACCTCAGTGG CCATGACCCG CTCTGGTCTC TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT 
CAAGGTCAGA GTTCAGAGGT CATTACGCCT TCAGAGGAGA TGAACAGAAT CAATGAAATG 
GACCTAAAGC TGGGGAACGT GGACACAAAA CTGGGAGACG GTTTGGCCGA GCATCTGGAA 
GTGGGCAGCA TTTTCACTTT CCGCGTCACC GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA 
TACGCAGACA TCTTCTGCCA GTTCAATTTC CTCCACCGTC ACGATGAGGC GTTCAGCACT 
GAGCCTCTGA AGAACACTGG CAGAGGAGCC CCGCTGGGCT TTTACCACGT ACAGAATATC 
TCAGTGGAGG TGACAGAGTC CTTCATTGAG TACATAAAGA CCAAGCCAAT TGTATTTGAA 
GTGTTTGGCC ATTACCAGCA ACACCCACTG CACATTCATG GACAGGATGT GGTCAGCCCT 
TCTCAGCCTT CCAGAAAGTA CTATCCTCCA CCAATGCCTC TGTCTAAACC AGTGCCAGCC 
ACCAAACTGA ACACCATCAC CAAGTCCAAC CTGGGTCAGT GTGTGAGCAA GTACGACTTG 
CTGGCCTGGT TTGAGATCAG CGAACTGGAG CCCACTGGAG AGTATATTCC AGCAGTAGTG 
GATCATACAG GAGGTCTTCC CTGTCATGGC ACATACCTGC TGCACCAGGG CATCCAGCGC 
AGAATAACAG TGACTCTGAT CCATGAGAAG GGAAGTGAGC TGCACTGGAA GGACGTCCGA 
GAGCTGGTGG TCGGTCGAAT CAGGAATAAG CCAGAAGTGG ATGATTCAGC CGCAGACGCC 
GTCTTGTCTC TCAACATCAT CTCAGCTAAA AACCTCAAAT CCTCCCACAA CTCCAGCAGG 
ACGTTCTACC GGTTTGAGGC CGTGTGGGAC AGTTCTCTGC ACAACTCTCT CCTACTGAAC 
CGCGTCACTC CATACGGAGA GAAGATCTAC ATGACCCTGT CTGCCTATCT GGAGCTCGAT 
CACTGCATCC AGCCTGCTAT CATCACCAAG GACATCTGTA TGGTCTTCTA CTCCAGAGAT 
GCCAAGATCT CGCCTCCTCG CTCGCTGAGA AACTTGTTTG GCAGCGGCTA CTCCAAAACC 
CCGGACTGTA ATAAAGTGAC TGGAGTCTAT GAGCTGAGCT TGTGCAAGAT AGCAGACACC 
GGAAGCCCAG GTATGCAGCG GCGCAGACGG AAGGTTCTGG ACACGTCAGT GGCATATGTG 
CGTGGAGAGG AGAATCTGGC CGGCTGGAGA CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC 
CAGTGGGAGC TGGACAAAAT GGAGCAGCTG CATGAGGTGG AAAAGACGCG TCACTTGCTT 
CTGCTGCGTG AGAAGCTGGG AGAAACACCT CCACCTAAAT CTCTGAGCGA GTCTCTGTCC 
CCCAGCCTGA GCAGCGGGAC CCTCAGCACC TCCACCAGCA TCTCCTCCCA GATCTCCTCC 
ACAACTTTCG AGAGTGCCAT CACCCCCAGC GAAAGCAGCG GCTATGACTC CACGGACATC 
GAGAGCTTGG TGGATCGAGA GAAGGAGCTG GCCACTAAGT GCCTCCGTCT CCTGACACAC 
ACTTTCAACA GTGAATACAA CCAAATGTGC AACAGCATTA GTGACTGCAA GTTTTCAGAT 
ATCTCTCCTA TGGGTCGGGA TCCATCAGTC AGTAGTTTCA GCAGTGCCAC CCTCACACCC 
TCCTCCACCT GCCCCTCGCT CTCAGACTCC AGGACCCCTG AAGCAAACTC TCGTGCAACC 
AGCCCCTCAG GCTCAGACTA TGAGAACTTC CCCATGGTGC CCATTCTGGA GACGTCCTAC 
CTGGCCCGCG CCGGCAAACA CGAGTTCCTC AACCTTGTGC CTAACATAGA GGAAATGAGG 
CCTGGGTCTG TGGTGTCCAA GAAGGGCATC TTGAATTTCA TGGAGCCCCG CTCGAACACC 
TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG CCGTATGTGT TCATCTACAA CAATGACAAG 
GACCCTGTGG AGAGAGGAGT GCTGAACTTA TCCACTGCAC AGGTGGAGTA CAGTGAAGAC 
CAGCAGGCCA TGCTCAAGAC GCCTCATACA TTTGCCATGT GCACCAAACA CAGAGGAATC 
CTGCTTCAGG CCAACAATGA CAAAGACATG AACGACTGGC TGTATGCCTT TAACCCACTG 
CTAGCAGGAA CAATAAGGTC GAAGCTTGCA AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295103.1
CDS	319..5358
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296828.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296828.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGAGCC TTCGTGTACC TGAGTAACCT GCTGTACCCT 
GTTCCTCTGG TGCATCGAGT CGCTGTAGTG ACAGAGAAAG GAGATGTGCG AGGATTTCTG 
CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT GAAGAGGCAC CTGATTATGG GTCTGGTGTG 
CGTCAGTCAG GAACTGCTAA GATTTCCTTT GATAATGAGT ATTTTAAAAA GAATGACTTC 
ACCTCAGTGG CCATGACCCG CTCTGGTCTC TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT 
CAAGGTCAGA GTTCAGAGGT CATTACGCCT TCAGAGGAGA TGAACAGAAT CAATGAAATG 
GACCTAAAGC TGGGGAACGT GGACACAAAA CTGGGAGACG GTTTGGCCGA GCATCTGGAA 
GTGGGCAGCA TTTTCACTTT CCGCGTCACC GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA 
TACGCAGACA TCTTCTGCCA GTTCAATTTC CTCCACCGTC ACGATGAGGC GTTCAGCACT 
GAGCCTCTGA AGAACACTGG CAGAGGAGCC CCGCTGGGCT TTTACCACGT ACAGAATATC 
TCAGTGGAGG TGACAGAGTC CTTCATTGAG TACATAAAGA CCAAGCCAAT TGTATTTGAA 
GTGTTTGGCC ATTACCAGCA ACACCCACTG CACATTCATG GACAGGATGT GGTCAGCCCT 
TCTCAGCCTT CCAGAAAGTA CTATCCTCCA CCAATGCCTC TGTCTAAACC AGTGCCAGCC 
ACCAAACTGA ACACCATCAC CAAGTCCAAC CTGGGTCAGT GTGTGAGCAA GTACGACTTG 
CTGGCCTGGT TTGAGATCAG CGAACTGGAG CCCACTGGAG AGTATATTCC AGCAGTAGTG 
GATCATACAG GAGGTCTTCC CTGTCATGGC ACATACCTGC TGCACCAGGG CATCCAGCGC 
AGAATAACAG TGACTCTGAT CCATGAGAAG GGAAGTGAGC TGCACTGGAA GGACGTCCGA 
GAGCTGGTGG TCGGTCGAAT CAGGAATAAG CCAGAAGTGG ATGATTCAGC CGCAGACGCC 
GTCTTGTCTC TCAACATCAT CTCAGCTAAA AACCTCAAAT CCTCCCACAA CTCCAGCAGG 
ACGTTCTACC GGTTTGAGGC CGTGTGGGAC AGTTCTCTGC ACAACTCTCT CCTACTGAAC 
CGCGTCACTC CATACGGAGA GAAGATCTAC ATGACCCTGT CTGCCTATCT GGAGCTCGAT 
CACTGCATCC AGCCTGCTAT CATCACCAAG GACATCTGTA TGGTCTTCTA CTCCAGAGAT 
GCCAAGATCT CGCCTCCTCG CTCGCTGAGA AACTTGTTTG GCAGCGGCTA CTCCAAAACC 
CCGGACTGTA ATAAAGTGAC TGGAGTCTAT GAGCTGAGCT TGTGCAAGAT AGCAGACACC 
GGAAGCCCAG GTATGCAGCG GCGCAGACGG AAGGTTCTGG ACACGTCAGT GGCATATGTG 
CGTGGAGAGG AGAATCTGGC CGGCTGGAGA CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC 
CAGTGGGAGC TGGACAAAAT GGAGCAGCTG CATGAGGTGG AAAAGACGCG TCACTTGCTT 
CTGCTGCGTG AGAAGCTGGG AGAAACACCT CCACCTAAAT CTCTGAGCGA GTCTCTGTCC 
CCCAGCCTGA GCAGCGGGAC CCTCAGCACC TCCACCAGCA TCTCCTCCCA GATCTCCTCC 
ACAACTTTCG AGAGTGCCAT CACCCCCAGC GAAAGCAGCG GCTATGACTC CACGGACATC 
GAGAGCTTGG TGGATCGAGA GAAGGAGCTG GCCACTAAGT GCCTCCGTCT CCTGACACAC 
ACTTTCAACA GTGAATACAA CCAAATGTGC AACAGCATTA GTGACTGCAA GTTTTCAGAT 
ATCTCTCCTA TGGGTCGGGA TCCATCAGTC AGTAGTTTCA GCAGTGCCAC CCTCACACCC 
TCCTCCACCT GCCCCTCGCT CTCAGACTCC AGGACCCCTG AAGCAAACTC TCGTGCAACC 
AGCCCCTCAG GCTCAGACTA TGAGAACTTC CCCATGGTGC CCATTCTGGA GACGTCCTAC 
CTGGCCCGCG CCGGCAAACA CGAGTTCCTC AACCTTGTGC CTAACATAGA GGAAATGAGG 
CCTGGGTCTG TGGTGTCCAA GAAGGGCATC TTGAATTTCA TGGAGCCCCG CTCGAACACC 
TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG CCGTATGTGT TCATCTACAA CAATGACAAG 
GACCCTGTGG AGAGAGGAGT GCTGAACTTA TCCACTGCAC AGGTGGAGTA CAGTGAAGAC 
CAGCAGGCCA TGCTCAAGAC GCCTCATACA TTTGCCATGT GCACCAAACA CAGAGGAATC 
CTGCTTCAGG CCAACAATGA CAAAGACATG AACGACTGGC TGTATGCCTT TAACCCACTG 
CTAGCAGGAA CAATAAGGTC GAAGCTTGCA AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296828.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295103.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.

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CloneID	ODa40276
Clone ID Related Accession (Same CDS sequence)	XM_009296828.3 , XM_009296828.2
Accession Version	XM_009296828.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGAGCC TTCGTGTACC TGAGTAACCT GCTGTACCCT 
GTTCCTCTGG TGCATCGAGT CGCTGTAGTG ACAGAGAAAG GAGATGTGCG AGGATTTCTG 
CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT GAAGAGGCAC CTGATTATGG GTCTGGTGTG 
CGTCAGTCAG GAACTGCTAA GATTTCCTTT GATAATGAGT ATTTTAAAAA GAATGACTTC 
ACCTCAGTGG CCATGACCCG CTCTGGTCTC TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT 
CAAGGTCAGA GTTCAGAGGT CATTACGCCT TCAGAGGAGA TGAACAGAAT CAATGAAATG 
GACCTAAAGC TGGGGAACGT GGACACAAAA CTGGGAGACG GTTTGGCCGA GCATCTGGAA 
GTGGGCAGCA TTTTCACTTT CCGCGTCACC GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA 
TACGCAGACA TCTTCTGCCA GTTCAATTTC CTCCACCGTC ACGATGAGGC GTTCAGCACT 
GAGCCTCTGA AGAACACTGG CAGAGGAGCC CCGCTGGGCT TTTACCACGT ACAGAATATC 
TCAGTGGAGG TGACAGAGTC CTTCATTGAG TACATAAAGA CCAAGCCAAT TGTATTTGAA 
GTGTTTGGCC ATTACCAGCA ACACCCACTG CACATTCATG GACAGGATGT GGTCAGCCCT 
TCTCAGCCTT CCAGAAAGTA CTATCCTCCA CCAATGCCTC TGTCTAAACC AGTGCCAGCC 
ACCAAACTGA ACACCATCAC CAAGTCCAAC CTGGGTCAGT GTGTGAGCAA GTACGACTTG 
CTGGCCTGGT TTGAGATCAG CGAACTGGAG CCCACTGGAG AGTATATTCC AGCAGTAGTG 
GATCATACAG GAGGTCTTCC CTGTCATGGC ACATACCTGC TGCACCAGGG CATCCAGCGC 
AGAATAACAG TGACTCTGAT CCATGAGAAG GGAAGTGAGC TGCACTGGAA GGACGTCCGA 
GAGCTGGTGG TCGGTCGAAT CAGGAATAAG CCAGAAGTGG ATGATTCAGC CGCAGACGCC 
GTCTTGTCTC TCAACATCAT CTCAGCTAAA AACCTCAAAT CCTCCCACAA CTCCAGCAGG 
ACGTTCTACC GGTTTGAGGC CGTGTGGGAC AGTTCTCTGC ACAACTCTCT CCTACTGAAC 
CGCGTCACTC CATACGGAGA GAAGATCTAC ATGACCCTGT CTGCCTATCT GGAGCTCGAT 
CACTGCATCC AGCCTGCTAT CATCACCAAG GACATCTGTA TGGTCTTCTA CTCCAGAGAT 
GCCAAGATCT CGCCTCCTCG CTCGCTGAGA AACTTGTTTG GCAGCGGCTA CTCCAAAACC 
CCGGACTGTA ATAAAGTGAC TGGAGTCTAT GAGCTGAGCT TGTGCAAGAT AGCAGACACC 
GGAAGCCCAG GTATGCAGCG GCGCAGACGG AAGGTTCTGG ACACGTCAGT GGCATATGTG 
CGTGGAGAGG AGAATCTGGC CGGCTGGAGA CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC 
CAGTGGGAGC TGGACAAAAT GGAGCAGCTG CATGAGGTGG AAAAGACGCG TCACTTGCTT 
CTGCTGCGTG AGAAGCTGGG AGAAACACCT CCACCTAAAT CTCTGAGCGA GTCTCTGTCC 
CCCAGCCTGA GCAGCGGGAC CCTCAGCACC TCCACCAGCA TCTCCTCCCA GATCTCCTCC 
ACAACTTTCG AGAGTGCCAT CACCCCCAGC GAAAGCAGCG GCTATGACTC CACGGACATC 
GAGAGCTTGG TGGATCGAGA GAAGGAGCTG GCCACTAAGT GCCTCCGTCT CCTGACACAC 
ACTTTCAACA GTGAATACAA CCAAATGTGC AACAGCATTA GTGACTGCAA GTTTTCAGAT 
ATCTCTCCTA TGGGTCGGGA TCCATCAGTC AGTAGTTTCA GCAGTGCCAC CCTCACACCC 
TCCTCCACCT GCCCCTCGCT CTCAGACTCC AGGACCCCTG AAGCAAACTC TCGTGCAACC 
AGCCCCTCAG GCTCAGACTA TGAGAACTTC CCCATGGTGC CCATTCTGGA GACGTCCTAC 
CTGGCCCGCG CCGGCAAACA CGAGTTCCTC AACCTTGTGC CTAACATAGA GGAAATGAGG 
CCTGGGTCTG TGGTGTCCAA GAAGGGCATC TTGAATTTCA TGGAGCCCCG CTCGAACACC 
TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG CCGTATGTGT TCATCTACAA CAATGACAAG 
GACCCTGTGG AGAGAGGAGT GCTGAACTTA TCCACTGCAC AGGTGGAGTA CAGTGAAGAC 
CAGCAGGCCA TGCTCAAGAC GCCTCATACA TTTGCCATGT GCACCAAACA CAGAGGAATC 
CTGCTTCAGG CCAACAATGA CAAAGACATG AACGACTGGC TGTATGCCTT TAACCCACTG 
CTAGCAGGAA CAATAAGGTC GAAGCTTGCA AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295103.1
CDS	319..5358
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296828.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296828.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GAGCAAGAAG AAAAAGAAGA CCGACTTCAT TCCTTACCGA 
GACTCAGTGT TGACATGGCT GCTAAGAGAG AATTTAGGAG GCAATTCGAG GACCGCAATG 
GTGGCTGCAC TGAGCCCTGC TGACATCAAC TTTGATGAAA CGCTCAGCAC ACTCCGATAT 
GCGGACAGAG CCAAGCAGAT CAAGTGCAAT GCTGTCATCA ACGAGGACCC CAATGCCAAA 
CTGGTGCGGG AGCTGAAGGA CGAGGTGTCA CGGCTGAAGG AGCTACTCCG TGCCCAAGGC 
CTCGGGGACA TCCTGGACAC ACCCATGGGC TGTCTGACAG CCTCTCCATC TTCAGGCTCT 
CTCTGCAGCC AGGCAGGCCT ACAGTCCGTC TCCAGCATCC AGGAGCGCAT CATGTCCACG 
CCAGGCGGAG AAGAGGCCAT CGAGAGGCTC AAGGAATCAG AAAAGATCAT AGCCGAGCTG 
AATGAGACCT GGGAAGAGAA GCTGAGGAAG ACCGAGGCCA TCCGTATGGA GAGAGAGGCG 
CTGCTGGCAG AGATGGGAGT TGCCATACGG GAGGACGGGG GAACCCTGGG CGTCTTCTCT 
CCTAAAAAGA CGCCTCACCT GGTGAACCTG AACGAGGATC CTTTGATGTC AGAATGTCTG 
CTCTATTATA TCAAAGATGG AATAACAAGG GTGGGCCAGG CAGATGCTGA AAGAAGACAG 
GATATCGTTC TAAGTGGAGC TCACATTAAA GAGGAGCACT GTATTTTCCG CAGTGAGAGG 
AATGCCAATG GACATGTGAT TGTCACGTTG GAGCCATGTG AGGGATCAGA AACATATGTA 
AATGGTAAAC GTGTGAATTC TGCGGTCCAG CTGCGCTCAG GTAACCGCAT TATCATGGGA 
AAGAACCACG TGTTCCGGTT TAACCACCCT GAGCAGGCTC GCGCTGAGCG TGAGAAGACC 
CCGTCAGCAG AGACACCTGT AGAACCTGTT GACTGGACTT TTGCCCAAAG AGAACTGCTG 
GAGAAACAGG GTATCGACAT GAAGCAAGAG ATGGAGAAGA GGCTTACAGA AATGGAGATC 
CTGTACAAAA AAGAGAAGGA GGAAGCGGAC CAGCTACTGG AGCAACAGAG ACTGGTGTAT 
GAGAGCAAAC TGCAAGAGCT GCAGAAACAA GTGGAGACTC GTTCTTTGGC TGCAGAGACT 
CCAGATGAGG AGGAAGAAGA AGAGGAGGAG GATGAAGTGC CCTGGACCCA GCATAAATAT 
GAGCTGGCTC AGTGGGCTTT CAGGAAGTGG AGATACCACC AGTTCACCTC CCTCAGAGAC 
CAATTATGGG GCAATGCTGT GTACCTCAAA GAAGCCAATG CCATCAGTGT GGAGCTCAAG 
AAAAAGGTCC AGTTCCAGTT CGTCTTGCTC ACAGACACCC TCTATTCTCC ACTGCCCCCG 
GAGCTGCTGC CTCCAGAGCC AGAGAAAGAG CGAGACGCCA GGCCTTTTCC GAGAACAGTG 
GTGGCTGTGG AGGTTCAGGA CCTGAAGAAT GGAGCCACTC ACTACTGGTC CCTGGAGAAG 
CTGAAGCAGA GGCTGGACCA GATGCGAGAG ATGTACGACC GTGCTGGCGA AATGGCCTCG 
TCCAATCAGG GAGATGATGG GGAGGGTGCG TTGACCGGCA GTGACCCGTT CTATGACCGA 
TTTCATTGGT TTAAGCTGGT TGGGAGAGCC TTCGTGTACC TGAGTAACCT GCTGTACCCT 
GTTCCTCTGG TGCATCGAGT CGCTGTAGTG ACAGAGAAAG GAGATGTGCG AGGATTTCTG 
CGTGTGGGGG TCCAGGCCAT CGCCGCGGAT GAAGAGGCAC CTGATTATGG GTCTGGTGTG 
CGTCAGTCAG GAACTGCTAA GATTTCCTTT GATAATGAGT ATTTTAAAAA GAATGACTTC 
ACCTCAGTGG CCATGACCCG CTCTGGTCTC TCTCTGGAGG AGCTGCGCAT TGTAGAAGGT 
CAAGGTCAGA GTTCAGAGGT CATTACGCCT TCAGAGGAGA TGAACAGAAT CAATGAAATG 
GACCTAAAGC TGGGGAACGT GGACACAAAA CTGGGAGACG GTTTGGCCGA GCATCTGGAA 
GTGGGCAGCA TTTTCACTTT CCGCGTCACC GTTCTGCAGG CAAGCGGCAT CCCGGCCGAA 
TACGCAGACA TCTTCTGCCA GTTCAATTTC CTCCACCGTC ACGATGAGGC GTTCAGCACT 
GAGCCTCTGA AGAACACTGG CAGAGGAGCC CCGCTGGGCT TTTACCACGT ACAGAATATC 
TCAGTGGAGG TGACAGAGTC CTTCATTGAG TACATAAAGA CCAAGCCAAT TGTATTTGAA 
GTGTTTGGCC ATTACCAGCA ACACCCACTG CACATTCATG GACAGGATGT GGTCAGCCCT 
TCTCAGCCTT CCAGAAAGTA CTATCCTCCA CCAATGCCTC TGTCTAAACC AGTGCCAGCC 
ACCAAACTGA ACACCATCAC CAAGTCCAAC CTGGGTCAGT GTGTGAGCAA GTACGACTTG 
CTGGCCTGGT TTGAGATCAG CGAACTGGAG CCCACTGGAG AGTATATTCC AGCAGTAGTG 
GATCATACAG GAGGTCTTCC CTGTCATGGC ACATACCTGC TGCACCAGGG CATCCAGCGC 
AGAATAACAG TGACTCTGAT CCATGAGAAG GGAAGTGAGC TGCACTGGAA GGACGTCCGA 
GAGCTGGTGG TCGGTCGAAT CAGGAATAAG CCAGAAGTGG ATGATTCAGC CGCAGACGCC 
GTCTTGTCTC TCAACATCAT CTCAGCTAAA AACCTCAAAT CCTCCCACAA CTCCAGCAGG 
ACGTTCTACC GGTTTGAGGC CGTGTGGGAC AGTTCTCTGC ACAACTCTCT CCTACTGAAC 
CGCGTCACTC CATACGGAGA GAAGATCTAC ATGACCCTGT CTGCCTATCT GGAGCTCGAT 
CACTGCATCC AGCCTGCTAT CATCACCAAG GACATCTGTA TGGTCTTCTA CTCCAGAGAT 
GCCAAGATCT CGCCTCCTCG CTCGCTGAGA AACTTGTTTG GCAGCGGCTA CTCCAAAACC 
CCGGACTGTA ATAAAGTGAC TGGAGTCTAT GAGCTGAGCT TGTGCAAGAT AGCAGACACC 
GGAAGCCCAG GTATGCAGCG GCGCAGACGG AAGGTTCTGG ACACGTCAGT GGCATATGTG 
CGTGGAGAGG AGAATCTGGC CGGCTGGAGA CCCCGAGGAG ACAGTCTGAT CCTGGAGCAC 
CAGTGGGAGC TGGACAAAAT GGAGCAGCTG CATGAGGTGG AAAAGACGCG TCACTTGCTT 
CTGCTGCGTG AGAAGCTGGG AGAAACACCT CCACCTAAAT CTCTGAGCGA GTCTCTGTCC 
CCCAGCCTGA GCAGCGGGAC CCTCAGCACC TCCACCAGCA TCTCCTCCCA GATCTCCTCC 
ACAACTTTCG AGAGTGCCAT CACCCCCAGC GAAAGCAGCG GCTATGACTC CACGGACATC 
GAGAGCTTGG TGGATCGAGA GAAGGAGCTG GCCACTAAGT GCCTCCGTCT CCTGACACAC 
ACTTTCAACA GTGAATACAA CCAAATGTGC AACAGCATTA GTGACTGCAA GTTTTCAGAT 
ATCTCTCCTA TGGGTCGGGA TCCATCAGTC AGTAGTTTCA GCAGTGCCAC CCTCACACCC 
TCCTCCACCT GCCCCTCGCT CTCAGACTCC AGGACCCCTG AAGCAAACTC TCGTGCAACC 
AGCCCCTCAG GCTCAGACTA TGAGAACTTC CCCATGGTGC CCATTCTGGA GACGTCCTAC 
CTGGCCCGCG CCGGCAAACA CGAGTTCCTC AACCTTGTGC CTAACATAGA GGAAATGAGG 
CCTGGGTCTG TGGTGTCCAA GAAGGGCATC TTGAATTTCA TGGAGCCCCG CTCGAACACC 
TGGGTGAAGC ACTTTGTGGT GGTGCGTCGG CCGTATGTGT TCATCTACAA CAATGACAAG 
GACCCTGTGG AGAGAGGAGT GCTGAACTTA TCCACTGCAC AGGTGGAGTA CAGTGAAGAC 
CAGCAGGCCA TGCTCAAGAC GCCTCATACA TTTGCCATGT GCACCAAACA CAGAGGAATC 
CTGCTTCAGG CCAACAATGA CAAAGACATG AACGACTGGC TGTATGCCTT TAACCCACTG 
CTAGCAGGAA CAATAAGGTC GAAGCTTGCA AGGAGACGGT CTGGTGTGAT GAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296832.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295107.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X23, mRNA.

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CloneID	ODa40280
Clone ID Related Accession (Same CDS sequence)	XM_009296832.3 , XM_009296832.2
Accession Version	XM_009296832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF1B isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG TGCCAGCCAC CAAACTGAAC ACCATCACCA AGTCCAACCT GGGTCAGTGT 
GTGAGCAAGT ACGACTTGCT GGCCTGGTTT GAGATCAGCG AACTGGAGCC CACTGGAGAG 
TATATTCCAG CAGTAGTGGA TCATACAGGA GGTCTTCCCT GTCATGGCAC ATACCTGCTG 
CACCAGGGCA TCCAGCGCAG AATAACAGTG ACTCTGATCC ATGAGAAGGG AAGTGAGCTG 
CACTGGAAGG ACGTCCGAGA GCTGGTGGTC GGTCGAATCA GGAATAAGCC AGAAGTGGAT 
GATTCAGCCG CAGACGCCGT CTTGTCTCTC AACATCATCT CAGCTAAAAA CCTCAAATCC 
TCCCACAACT CCAGCAGGAC GTTCTACCGG TTTGAGGCCG TGTGGGACAG TTCTCTGCAC 
AACTCTCTCC TACTGAACCG CGTCACTCCA TACGGAGAGA AGATCTACAT GACCCTGTCT 
GCCTATCTGG AGCTCGATCA CTGCATCCAG CCTGCTATCA TCACCAAGGA CATCTGTATG 
GTCTTCTACT CCAGAGATGC CAAGATCTCG CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC 
AGCGGCTACT CCAAAACCCC GGACTGTAAT AAAGTGACTG GAGTCTATGA GCTGAGCTTG 
TGCAAGATAG CAGACACCGG AAGCCCAGGT ATGCAGCGGC GCAGACGGAA GGTTCTGGAC 
ACGTCAGTGG CATATGTGCG TGGAGAGGAG AATCTGGCCG GCTGGAGACC CCGAGGAGAC 
AGTCTGATCC TGGAGCACCA GTGGGAGCTG GACAAAATGG AGCAGCTGCA TGAGGTGGAA 
AAGACGCGTC ACTTGCTTCT GCTGCGTGAG AAGCTGGGAG AAACACCTCC ACCTAAATCT 
CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC AGCGGGACCC TCAGCACCTC CACCAGCATC 
TCCTCCCAGA TCTCCTCCAC AACTTTCGAG AGTGCCATCA CCCCCAGCGA AAGCAGCGGC 
TATGACTCCA CGGACATCGA GAGCTTGGTG GATCGAGAGA AGGAGCTGGC CACTAAGTGC 
CTCCGTCTCC TGACACACAC TTTCAACAGT GAATACAACC AAATGTGCAA CAGCATTAGT 
GACTGCAAGT TTTCAGATAT CTCTCCTATG GGTCGGGATC CATCAGTCAG TAGTTTCAGC 
AGTGCCACCC TCACACCCTC CTCCACCTGC CCCTCGCTCT CAGACTCCAG GACCCCTGAA 
GCAAACTCTC GTGCAACCAG CCCCTCAGGC TCAGACTATG AGAACTTCCC CATGGTGCCC 
ATTCTGGAGA CGTCCTACCT GGCCCGCGCC GGCAAACACG AGTTCCTCAA CCTTGTGCCT 
AACATAGAGG AAATGAGGCC TGGGTCTGTG GTGTCCAAGA AGGGCATCTT GAATTTCATG 
GAGCCCCGCT CGAACACCTG GGTGAAGCAC TTTGTGGTGG TGCGTCGGCC GTATGTGTTC 
ATCTACAACA ATGACAAGGA CCCTGTGGAG AGAGGAGTGC TGAACTTATC CACTGCACAG 
GTGGAGTACA GTGAAGACCA GCAGGCCATG CTCAAGACGC CTCATACATT TGCCATGTGC 
ACCAAACACA GAGGAATCCT GCTTCAGGCC AACAATGACA AAGACATGAA CGACTGGCTG 
TATGCCTTTA ACCCACTGCT AGCAGGAACA ATAAGGTCGA AGCTTGCAAG GAGACGGTCT 
GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295107.1
CDS	319..5376
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296832.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296832.3

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG TGCCAGCCAC CAAACTGAAC ACCATCACCA AGTCCAACCT GGGTCAGTGT 
GTGAGCAAGT ACGACTTGCT GGCCTGGTTT GAGATCAGCG AACTGGAGCC CACTGGAGAG 
TATATTCCAG CAGTAGTGGA TCATACAGGA GGTCTTCCCT GTCATGGCAC ATACCTGCTG 
CACCAGGGCA TCCAGCGCAG AATAACAGTG ACTCTGATCC ATGAGAAGGG AAGTGAGCTG 
CACTGGAAGG ACGTCCGAGA GCTGGTGGTC GGTCGAATCA GGAATAAGCC AGAAGTGGAT 
GATTCAGCCG CAGACGCCGT CTTGTCTCTC AACATCATCT CAGCTAAAAA CCTCAAATCC 
TCCCACAACT CCAGCAGGAC GTTCTACCGG TTTGAGGCCG TGTGGGACAG TTCTCTGCAC 
AACTCTCTCC TACTGAACCG CGTCACTCCA TACGGAGAGA AGATCTACAT GACCCTGTCT 
GCCTATCTGG AGCTCGATCA CTGCATCCAG CCTGCTATCA TCACCAAGGA CATCTGTATG 
GTCTTCTACT CCAGAGATGC CAAGATCTCG CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC 
AGCGGCTACT CCAAAACCCC GGACTGTAAT AAAGTGACTG GAGTCTATGA GCTGAGCTTG 
TGCAAGATAG CAGACACCGG AAGCCCAGGT ATGCAGCGGC GCAGACGGAA GGTTCTGGAC 
ACGTCAGTGG CATATGTGCG TGGAGAGGAG AATCTGGCCG GCTGGAGACC CCGAGGAGAC 
AGTCTGATCC TGGAGCACCA GTGGGAGCTG GACAAAATGG AGCAGCTGCA TGAGGTGGAA 
AAGACGCGTC ACTTGCTTCT GCTGCGTGAG AAGCTGGGAG AAACACCTCC ACCTAAATCT 
CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC AGCGGGACCC TCAGCACCTC CACCAGCATC 
TCCTCCCAGA TCTCCTCCAC AACTTTCGAG AGTGCCATCA CCCCCAGCGA AAGCAGCGGC 
TATGACTCCA CGGACATCGA GAGCTTGGTG GATCGAGAGA AGGAGCTGGC CACTAAGTGC 
CTCCGTCTCC TGACACACAC TTTCAACAGT GAATACAACC AAATGTGCAA CAGCATTAGT 
GACTGCAAGT TTTCAGATAT CTCTCCTATG GGTCGGGATC CATCAGTCAG TAGTTTCAGC 
AGTGCCACCC TCACACCCTC CTCCACCTGC CCCTCGCTCT CAGACTCCAG GACCCCTGAA 
GCAAACTCTC GTGCAACCAG CCCCTCAGGC TCAGACTATG AGAACTTCCC CATGGTGCCC 
ATTCTGGAGA CGTCCTACCT GGCCCGCGCC GGCAAACACG AGTTCCTCAA CCTTGTGCCT 
AACATAGAGG AAATGAGGCC TGGGTCTGTG GTGTCCAAGA AGGGCATCTT GAATTTCATG 
GAGCCCCGCT CGAACACCTG GGTGAAGCAC TTTGTGGTGG TGCGTCGGCC GTATGTGTTC 
ATCTACAACA ATGACAAGGA CCCTGTGGAG AGAGGAGTGC TGAACTTATC CACTGCACAG 
GTGGAGTACA GTGAAGACCA GCAGGCCATG CTCAAGACGC CTCATACATT TGCCATGTGC 
ACCAAACACA GAGGAATCCT GCTTCAGGCC AACAATGACA AAGACATGAA CGACTGGCTG 
TATGCCTTTA ACCCACTGCT AGCAGGAACA ATAAGGTCGA AGCTTGCAAG GAGACGGTCT 
GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_009296832.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295107.1 Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.

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CloneID	ODa40280
Clone ID Related Accession (Same CDS sequence)	XM_009296832.3 , XM_009296832.2
Accession Version	XM_009296832.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF1B isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG TGCCAGCCAC CAAACTGAAC ACCATCACCA AGTCCAACCT GGGTCAGTGT 
GTGAGCAAGT ACGACTTGCT GGCCTGGTTT GAGATCAGCG AACTGGAGCC CACTGGAGAG 
TATATTCCAG CAGTAGTGGA TCATACAGGA GGTCTTCCCT GTCATGGCAC ATACCTGCTG 
CACCAGGGCA TCCAGCGCAG AATAACAGTG ACTCTGATCC ATGAGAAGGG AAGTGAGCTG 
CACTGGAAGG ACGTCCGAGA GCTGGTGGTC GGTCGAATCA GGAATAAGCC AGAAGTGGAT 
GATTCAGCCG CAGACGCCGT CTTGTCTCTC AACATCATCT CAGCTAAAAA CCTCAAATCC 
TCCCACAACT CCAGCAGGAC GTTCTACCGG TTTGAGGCCG TGTGGGACAG TTCTCTGCAC 
AACTCTCTCC TACTGAACCG CGTCACTCCA TACGGAGAGA AGATCTACAT GACCCTGTCT 
GCCTATCTGG AGCTCGATCA CTGCATCCAG CCTGCTATCA TCACCAAGGA CATCTGTATG 
GTCTTCTACT CCAGAGATGC CAAGATCTCG CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC 
AGCGGCTACT CCAAAACCCC GGACTGTAAT AAAGTGACTG GAGTCTATGA GCTGAGCTTG 
TGCAAGATAG CAGACACCGG AAGCCCAGGT ATGCAGCGGC GCAGACGGAA GGTTCTGGAC 
ACGTCAGTGG CATATGTGCG TGGAGAGGAG AATCTGGCCG GCTGGAGACC CCGAGGAGAC 
AGTCTGATCC TGGAGCACCA GTGGGAGCTG GACAAAATGG AGCAGCTGCA TGAGGTGGAA 
AAGACGCGTC ACTTGCTTCT GCTGCGTGAG AAGCTGGGAG AAACACCTCC ACCTAAATCT 
CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC AGCGGGACCC TCAGCACCTC CACCAGCATC 
TCCTCCCAGA TCTCCTCCAC AACTTTCGAG AGTGCCATCA CCCCCAGCGA AAGCAGCGGC 
TATGACTCCA CGGACATCGA GAGCTTGGTG GATCGAGAGA AGGAGCTGGC CACTAAGTGC 
CTCCGTCTCC TGACACACAC TTTCAACAGT GAATACAACC AAATGTGCAA CAGCATTAGT 
GACTGCAAGT TTTCAGATAT CTCTCCTATG GGTCGGGATC CATCAGTCAG TAGTTTCAGC 
AGTGCCACCC TCACACCCTC CTCCACCTGC CCCTCGCTCT CAGACTCCAG GACCCCTGAA 
GCAAACTCTC GTGCAACCAG CCCCTCAGGC TCAGACTATG AGAACTTCCC CATGGTGCCC 
ATTCTGGAGA CGTCCTACCT GGCCCGCGCC GGCAAACACG AGTTCCTCAA CCTTGTGCCT 
AACATAGAGG AAATGAGGCC TGGGTCTGTG GTGTCCAAGA AGGGCATCTT GAATTTCATG 
GAGCCCCGCT CGAACACCTG GGTGAAGCAC TTTGTGGTGG TGCGTCGGCC GTATGTGTTC 
ATCTACAACA ATGACAAGGA CCCTGTGGAG AGAGGAGTGC TGAACTTATC CACTGCACAG 
GTGGAGTACA GTGAAGACCA GCAGGCCATG CTCAAGACGC CTCATACATT TGCCATGTGC 
ACCAAACACA GAGGAATCCT GCTTCAGGCC AACAATGACA AAGACATGAA CGACTGGCTG 
TATGCCTTTA ACCCACTGCT AGCAGGAACA ATAAGGTCGA AGCTTGCAAG GAGACGGTCT 
GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295107.1
CDS	319..5376
Translation	MSGASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEPKTFSFDYSYWSHTSPDDPSFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKINDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGDILDTPMGCLTASPSSGSLCSQAGLQSVSSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKRVNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPVEPVDWTFAQRELLEKQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSLAAETPDEEEEEEEEDEVPWTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFDNEYFKKNDFTSVAMTRSGLSLEELRIVEGQGQSSEVITPSEEMNRINEMDLKLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTGRGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHGQDVVSPSQPSRKYYPPPMPLSKPVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLLHQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPEVDDSAADAVLSLNIISAKNLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDICMVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVAYVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLGETPPPKSLSESLSPSLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLVDREKELATKCLRLLTHTFNSEYNQMCNSISDCKFSDISPMGRDPSVSSFSSATLTPSSTCPSLSDSRTPEANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNLVPNIEEMRPGSVVSKKGILNFMEPRSNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILLQANNDKDMNDWLYAFNPLLAGTIRSKLARRRSGVMKN

Target ORF information:

RefSeq Version	XM_009296832.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 1B (kif1b), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296832.2

ORF Insert Sequence:

ATGTCTGGCG CCTCAGTGAA AGTGGCTGTC AGAGTTCGGC CCTTCAACTC AAGGGAGACC 
GGCAAAGAAT CAAAATGCAT TATACAGATG CAAGGGAACT CCACCACTAT TTTGAACCCC 
AAGAACCCAA AGGAACCCAA AACCTTCAGC TTTGACTACT CCTACTGGTC TCACACCTCA 
CCGGACGACC CCAGCTTTGC GTCACAAAAC CAAGTCTACA ATGACATCGG GAAAGAGATG 
CTGCAGCATG CTTTTGAAGG ATATAACGTC TGCATTTTTG CATATGGCCA GACTGGAGCT 
GGCAAGTCCT ATACCATGAT GGGAAAGCAA GAGGAAGGAC AGGAAGGCAT TATTCCTCAG 
CTCTGTGAAG AACTCTTCGA AAAAATCAAT GACAATAACA ATGAGGAGAT CTCGTACTCG 
GTGGAGGTGG CTTATATGGA GATATACTGT GAGCGGGTGA GGGACTTGCT GAACCCTAAG 
AACAAAGGGA ACCTTCGTGT CCGGGAGCAT CCTCTACTTG GGCCGTATGT TGAAGACCTC 
TCGAAACTGG CTGTCACCTC GTACACCGAC ATAGCTGACC TGATGGATGC TGGTAACAAA 
GCCAGAACTG TCGCTGCAAC CAACATGAAC GAGACGAGCA GTCGTTCTCA CGCCGTCTTT 
ACTATCGTTT TCACTCAGAG GAAGTACGAC AGCGAGACGG ACCTCTCCAC CGAGAAGGTC 
AGCAAAATCA GCCTGGTGGA TCTGGCAGGA AGTGAGCGTG CTGACTCAAC AGGGGCCAAA 
GGAACCAGAC TAAAGGAGGG AGCAAACATC AATAAATCTT TAACTACACT AGGAAAGGTG 
ATTTCTGCCC TGGCTGAGGT GGATAACTGT ACCAGCAAGA GCAAGAAGAA AAAGAAGACC 
GACTTCATTC CTTACCGAGA CTCAGTGTTG ACATGGCTGC TAAGAGAGAA TTTAGGAGGC 
AATTCGAGGA CCGCAATGGT GGCTGCACTG AGCCCTGCTG ACATCAACTT TGATGAAACG 
CTCAGCACAC TCCGATATGC GGACAGAGCC AAGCAGATCA AGTGCAATGC TGTCATCAAC 
GAGGACCCCA ATGCCAAACT GGTGCGGGAG CTGAAGGACG AGGTGTCACG GCTGAAGGAG 
CTACTCCGTG CCCAAGGCCT CGGGGACATC CTGGACACAC CCATGGGCTG TCTGACAGCC 
TCTCCATCTT CAGGCTCTCT CTGCAGCCAG GCAGGCCTAC AGTCCGTCTC CAGCATCCAG 
GAGCGCATCA TGTCCACGCC AGGCGGAGAA GAGGCCATCG AGAGGCTCAA GGAATCAGAA 
AAGATCATAG CCGAGCTGAA TGAGACCTGG GAAGAGAAGC TGAGGAAGAC CGAGGCCATC 
CGTATGGAGA GAGAGGCGCT GCTGGCAGAG ATGGGAGTTG CCATACGGGA GGACGGGGGA 
ACCCTGGGCG TCTTCTCTCC TAAAAAGACG CCTCACCTGG TGAACCTGAA CGAGGATCCT 
TTGATGTCAG AATGTCTGCT CTATTATATC AAAGATGGAA TAACAAGGGT GGGCCAGGCA 
GATGCTGAAA GAAGACAGGA TATCGTTCTA AGTGGAGCTC ACATTAAAGA GGAGCACTGT 
ATTTTCCGCA GTGAGAGGAA TGCCAATGGA CATGTGATTG TCACGTTGGA GCCATGTGAG 
GGATCAGAAA CATATGTAAA TGGTAAACGT GTGAATTCTG CGGTCCAGCT GCGCTCAGGT 
AACCGCATTA TCATGGGAAA GAACCACGTG TTCCGGTTTA ACCACCCTGA GCAGGCTCGC 
GCTGAGCGTG AGAAGACCCC GTCAGCAGAG ACACCTGTAG AACCTGTTGA CTGGACTTTT 
GCCCAAAGAG AACTGCTGGA GAAACAGGGT ATCGACATGA AGCAAGAGAT GGAGAAGAGG 
CTTACAGAAA TGGAGATCCT GTACAAAAAA GAGAAGGAGG AAGCGGACCA GCTACTGGAG 
CAACAGAGAC TGGTGTATGA GAGCAAACTG CAAGAGCTGC AGAAACAAGT GGAGACTCGT 
TCTTTGGCTG CAGAGACTCC AGATGAGGAG GAAGAAGAAG AGGAGGAGGA TGAAGTGCCC 
TGGACCCAGC ATAAATATGA GCTGGCTCAG TGGGCTTTCA GGAAGTGGAG ATACCACCAG 
TTCACCTCCC TCAGAGACCA ATTATGGGGC AATGCTGTGT ACCTCAAAGA AGCCAATGCC 
ATCAGTGTGG AGCTCAAGAA AAAGGTCCAG TTCCAGTTCG TCTTGCTCAC AGACACCCTC 
TATTCTCCAC TGCCCCCGGA GCTGCTGCCT CCAGAGCCAG AGAAAGAGCG AGACGCCAGG 
CCTTTTCCGA GAACAGTGGT GGCTGTGGAG GTTCAGGACC TGAAGAATGG AGCCACTCAC 
TACTGGTCCC TGGAGAAGCT GAAGCAGAGG CTGGACCAGA TGCGAGAGAT GTACGACCGT 
GCTGGCGAAA TGGCCTCGTC CAATCAGGGA GATGATGGGG AGGGTGCGTT GACCGGCAGT 
GACCCGTTCT ATGACCGATT TCATTGGTTT AAGCTGGTTG GGAGAGCCTT CGTGTACCTG 
AGTAACCTGC TGTACCCTGT TCCTCTGGTG CATCGAGTCG CTGTAGTGAC AGAGAAAGGA 
GATGTGCGAG GATTTCTGCG TGTGGGGGTC CAGGCCATCG CCGCGGATGA AGAGGCACCT 
GATTATGGGT CTGGTGTGCG TCAGTCAGGA ACTGCTAAGA TTTCCTTTGA TAATGAGTAT 
TTTAAAAAGA ATGACTTCAC CTCAGTGGCC ATGACCCGCT CTGGTCTCTC TCTGGAGGAG 
CTGCGCATTG TAGAAGGTCA AGGTCAGAGT TCAGAGGTCA TTACGCCTTC AGAGGAGATG 
AACAGAATCA ATGAAATGGA CCTAAAGCTG GGGAACGTGG ACACAAAACT GGGAGACGGT 
TTGGCCGAGC ATCTGGAAGT GGGCAGCATT TTCACTTTCC GCGTCACCGT TCTGCAGGCA 
AGCGGCATCC CGGCCGAATA CGCAGACATC TTCTGCCAGT TCAATTTCCT CCACCGTCAC 
GATGAGGCGT TCAGCACTGA GCCTCTGAAG AACACTGGCA GAGGAGCCCC GCTGGGCTTT 
TACCACGTAC AGAATATCTC AGTGGAGGTG ACAGAGTCCT TCATTGAGTA CATAAAGACC 
AAGCCAATTG TATTTGAAGT GTTTGGCCAT TACCAGCAAC ACCCACTGCA CATTCATGGA 
CAGGATGTGG TCAGCCCTTC TCAGCCTTCC AGAAAGTACT ATCCTCCACC AATGCCTCTG 
TCTAAACCAG TGCCAGCCAC CAAACTGAAC ACCATCACCA AGTCCAACCT GGGTCAGTGT 
GTGAGCAAGT ACGACTTGCT GGCCTGGTTT GAGATCAGCG AACTGGAGCC CACTGGAGAG 
TATATTCCAG CAGTAGTGGA TCATACAGGA GGTCTTCCCT GTCATGGCAC ATACCTGCTG 
CACCAGGGCA TCCAGCGCAG AATAACAGTG ACTCTGATCC ATGAGAAGGG AAGTGAGCTG 
CACTGGAAGG ACGTCCGAGA GCTGGTGGTC GGTCGAATCA GGAATAAGCC AGAAGTGGAT 
GATTCAGCCG CAGACGCCGT CTTGTCTCTC AACATCATCT CAGCTAAAAA CCTCAAATCC 
TCCCACAACT CCAGCAGGAC GTTCTACCGG TTTGAGGCCG TGTGGGACAG TTCTCTGCAC 
AACTCTCTCC TACTGAACCG CGTCACTCCA TACGGAGAGA AGATCTACAT GACCCTGTCT 
GCCTATCTGG AGCTCGATCA CTGCATCCAG CCTGCTATCA TCACCAAGGA CATCTGTATG 
GTCTTCTACT CCAGAGATGC CAAGATCTCG CCTCCTCGCT CGCTGAGAAA CTTGTTTGGC 
AGCGGCTACT CCAAAACCCC GGACTGTAAT AAAGTGACTG GAGTCTATGA GCTGAGCTTG 
TGCAAGATAG CAGACACCGG AAGCCCAGGT ATGCAGCGGC GCAGACGGAA GGTTCTGGAC 
ACGTCAGTGG CATATGTGCG TGGAGAGGAG AATCTGGCCG GCTGGAGACC CCGAGGAGAC 
AGTCTGATCC TGGAGCACCA GTGGGAGCTG GACAAAATGG AGCAGCTGCA TGAGGTGGAA 
AAGACGCGTC ACTTGCTTCT GCTGCGTGAG AAGCTGGGAG AAACACCTCC ACCTAAATCT 
CTGAGCGAGT CTCTGTCCCC CAGCCTGAGC AGCGGGACCC TCAGCACCTC CACCAGCATC 
TCCTCCCAGA TCTCCTCCAC AACTTTCGAG AGTGCCATCA CCCCCAGCGA AAGCAGCGGC 
TATGACTCCA CGGACATCGA GAGCTTGGTG GATCGAGAGA AGGAGCTGGC CACTAAGTGC 
CTCCGTCTCC TGACACACAC TTTCAACAGT GAATACAACC AAATGTGCAA CAGCATTAGT 
GACTGCAAGT TTTCAGATAT CTCTCCTATG GGTCGGGATC CATCAGTCAG TAGTTTCAGC 
AGTGCCACCC TCACACCCTC CTCCACCTGC CCCTCGCTCT CAGACTCCAG GACCCCTGAA 
GCAAACTCTC GTGCAACCAG CCCCTCAGGC TCAGACTATG AGAACTTCCC CATGGTGCCC 
ATTCTGGAGA CGTCCTACCT GGCCCGCGCC GGCAAACACG AGTTCCTCAA CCTTGTGCCT 
AACATAGAGG AAATGAGGCC TGGGTCTGTG GTGTCCAAGA AGGGCATCTT GAATTTCATG 
GAGCCCCGCT CGAACACCTG GGTGAAGCAC TTTGTGGTGG TGCGTCGGCC GTATGTGTTC 
ATCTACAACA ATGACAAGGA CCCTGTGGAG AGAGGAGTGC TGAACTTATC CACTGCACAG 
GTGGAGTACA GTGAAGACCA GCAGGCCATG CTCAAGACGC CTCATACATT TGCCATGTGC 
ACCAAACACA GAGGAATCCT GCTTCAGGCC AACAATGACA AAGACATGAA CGACTGGCTG 
TATGCCTTTA ACCCACTGCT AGCAGGAACA ATAAGGTCGA AGCTTGCAAG GAGACGGTCT 
GGTGTGATGA AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(26)7014-26(2016 06)
Drerup CM,Lusk S,Nechiporuk A

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Unique function of Kinesin Kif5A in localization of mitochondria in axons.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(44)14717-32(2014 Oct)
Campbell PD,Shen K,Sapio MR,Glenn TD,Talbot WS,Marlow FL

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kif1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: