g2e3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	g2e3
Entrez Gene ID	368848
Full Name	G2/M-phase specific E3 ubiquitin protein ligase
Synonyms	sb:cb726,si:busm1-234g15.1,si:dz234g15.1,wu:fe02a06,wu:fe24c12
General protein information	Preferred Names G2/M-phase specific E3 ubiquitin protein ligase Names G2/M phase-specific E3 ubiquitin-protein ligase G2/M-phase specific E3 ubiquitin ligase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467121.1	XP_021322796.1	G2/M phase-specific E3 ubiquitin-protein ligase isoform X1
NM_001003822.1	NP_001003822.1	G2/M phase-specific E3 ubiquitin-protein ligase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	G2e3	NP_001161435.1
Bos taurus (cattle)	G2E3	NP_001033760.2
Xenopus tropicalis (tropical clawed frog)	g2e3	XP_002932878.1
Pan troglodytes (chimpanzee)	G2E3	XP_003314336.1
Danio rerio (zebrafish)	g2e3	NP_001003822.1
Rattus norvegicus (Norway rat)	G2e3	NP_001100196.1
Homo sapiens (human)	G2E3	NP_060239.2
	G2E3	XP_001114662.1
Canis lupus familiaris (dog)	G2E3	XP_547761.4
Gallus gallus (chicken)	G2E3	NP_001012918.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

A subtracted cDNA library from the zebrafish (Danio rerio) embryonic inner ear.
Coimbra RS, Weil D, Brottier P, Blanchard S, Levi M, Hardelin JP, Weissenbach J, Petit C
Genome research12(6)1007-11(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following g2e3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the g2e3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03773	NM_001003822.1 Latest version!	Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa149517	XM_021467121.1 Latest version!	Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

g2e3 ( NM_001003822.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003822.1 Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), mRNA.

Price & Availability↑

CloneID	ODa03773
Clone ID Related Accession (Same CDS sequence)	NM_001003822.1
Accession Version	NM_001003822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	G2/M phase-specific E3 ubiquitin-protein ligase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL596026.13. ##Evidence-Data-START## Transcript exon combination :: BC100036.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAAGCGT TAAATGAAAA GTCCGCTGAA ATAAAGCTGC AGGACAGAGA TCTCATTTGC 
TGTCTATGTA AGAGATCTGA GAATAATAAA GAGAAGTATG GAGAGAAGGT TCACCTTGAG 
CAGCACAACC TGGCTGTCCA CTTTTTTTGT CTGCTGATGT CTAGTGGAAT ATGCCAGAGA 
GGAGAGGAAG ATGAAGACGT GTATGGTTTT CTTGTGGGTG ACATTAAAAA GGAGATCCGC 
AGATCATCGA GGCTGAGATG CTTTCATTGC AAAAAGGCTG GTGCTTCAGT GGGCTGCTCC 
ATCAAAAGCT GTCGGCAAAT GGTCCACATG CCCTGTGGAT TAGAGCAGGA GTTTGTTTTC 
CAGTTCACAG GCTTGTTTCC GTCTTTCTGT AAAAAGCATG CGCCTACTCA GTCCTGTGAC 
TCCAGCCACA GTCTGCCCCA CTCCTGCTCC ATCTGTCTGG ATCCCATTGA ACCCATCCTC 
TCCTATCATG TCCTCAAATG CCCTGCATGT CATGGAAGCT GGTTTCACAG GAAATGTGTA 
CAGAATTATG CACACAGTGC CGCAATGTTT TTCTTTAAAT GCACACTCTG CAATAACAAG 
GAGCAGTTTC AGCAGGAAAT GCTCAGAATG GGAATATATA TACCAGAGCG TGATGCATCT 
TGGGAACTGG AGGAGAATGC ATTTGTAGAG TTATTGCAGG TCTACCACCG CTGTGATGCA 
GTGAAGTGTC TGTGTCACAA AGGTCGTGAA CACAGCTCAC AGTCAGGGTA TTTTGAAATA 
GTTCTCTGCA AGTTGTGTGG ATCCAGGGGA ACTCATCGAA AGTGCTCTAA TATTCGAGTT 
TATGAAGCTG ACTGGATGTG TGCTGACTGT AAAACTGCAG CTGAGGGCAA AACATCATTG 
CCATTGCCAA ATCATGTTGA ATCGCCACTG GCTATAAGAC AAGAGAGGAA GAGGCTGTTT 
AAATCGATCT CTGACATTCA CTCTTCAATC GTCGCTAAAA GGCAGTGTGT GCCAGCCACT 
TCTCCGGAGG TCTTAATGGA TCTGGCCTGT CAGATCTCAC AGCAGCAGTT CACAGAGGTG 
CTGGTGGGAG ATGATGATGG TGGCGTGATG GAAGCAGCTC TGCAGGTGCT GCGCCAAAGT 
GACTTTAATC CCTGCTGCAC ACTTTCTGTC AAATTCTCCA AGGACAAACT GAACAACAAC 
ATTAGAAACC AGCGTCGCTT CCTCAGGCGT CTCGTTTCAA AACTGCAGGT CTCAGACATT 
TTTGAGGGAT CCGATGGAGC TAAAAACCTG GCTCTGAACT CAAAAGCTCT GAGGGACGAT 
CTCTACTTTG ATGTCGGCAG TCTATTGGCC CTCTCTTTGG TTCACGGAGG TCCTCCTCTG 
GGCTTCTTCT CTCCTGCACT GTATCATAGT CTGTTCAACT ATCCCAAAAA CTACAGGCCT 
ACTTTAGAAG ACCTCGGAGA CACTGCTTTT GGGCACAAAA TCAGACAAAT CGCAGAAGCA 
AAGTCAATGA AGGAGCTGAG ACATGCGATG AAGTCAGCAT CACAATATTT GGAAGCTGCA 
GGCTGTTGGC GGAAGATCAG CAAACTCTCT GAGAAAGACA TGTTGGTGGA GGATGTACTG 
AATTTCTACC TGATCATCAG ACTGCAGCTT CCTCTGCAAA GATTCCGTGA AGGTTTGAGG 
ACACTAGGAA TGTTCGAGCA GGTCCAGATG TGCTCAGAGA CGTTCTTTCC AGTCTTCTGT 
GGACCTGTGG AAAGGCTGAC AGCTGAATCT GTCATGGAGC TCTTCACAAC CCGGTTATCA 
GAAGAAAAAG AAAAACAAGT TCTAGAAAAG ACCACGATAA GCTTTTGGAA ACAGTATCTG 
CATGAATGTG AAGAGGGTCA ATGTGCAGCA TCGCTGGAGG ACCTCCTAAC ATTTGCCACT 
GGTACAGATT TGGTTCCAGC CATCGGATTC AAGCCCACTC CGTCCATCTC ATTCCTCAGC 
TCACCAGACA ACTTGTGTGC TTTTCCTCAG AGCAACTGTG ATGCCAATCA TCTCATTCTG 
CCTACTCTAC CCTCTTACCA GCTCTTCAAG AAACATTTGG ATTACACAGT GTGTCAGTTC 
TCAGTTATGC AGGACATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003822.1
CDS	227..2347
Translation	MKALNEKSAEIKLQDRDLICCLCKRSENNKEKYGEKVHLEQHNLAVHFFCLLMSSGICQRGEEDEDVYGFLVGDIKKEIRRSSRLRCFHCKKAGASVGCSIKSCRQMVHMPCGLEQEFVFQFTGLFPSFCKKHAPTQSCDSSHSLPHSCSICLDPIEPILSYHVLKCPACHGSWFHRKCVQNYAHSAAMFFFKCTLCNNKEQFQQEMLRMGIYIPERDASWELEENAFVELLQVYHRCDAVKCLCHKGREHSSQSGYFEIVLCKLCGSRGTHRKCSNIRVYEADWMCADCKTAAEGKTSLPLPNHVESPLAIRQERKRLFKSISDIHSSIVAKRQCVPATSPEVLMDLACQISQQQFTEVLVGDDDGGVMEAALQVLRQSDFNPCCTLSVKFSKDKLNNNIRNQRRFLRRLVSKLQVSDIFEGSDGAKNLALNSKALRDDLYFDVGSLLALSLVHGGPPLGFFSPALYHSLFNYPKNYRPTLEDLGDTAFGHKIRQIAEAKSMKELRHAMKSASQYLEAAGCWRKISKLSEKDMLVEDVLNFYLIIRLQLPLQRFREGLRTLGMFEQVQMCSETFFPVFCGPVERLTAESVMELFTTRLSEEKEKQVLEKTTISFWKQYLHECEEGQCAASLEDLLTFATGTDLVPAIGFKPTPSISFLSSPDNLCAFPQSNCDANHLILPTLPSYQLFKKHLDYTVCQFSVMQDI

Target ORF information:

RefSeq Version	NM_001003822.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003822.1

ORF Insert Sequence:

ATGAAAGCGT TAAATGAAAA GTCCGCTGAA ATAAAGCTGC AGGACAGAGA TCTCATTTGC 
TGTCTATGTA AGAGATCTGA GAATAATAAA GAGAAGTATG GAGAGAAGGT TCACCTTGAG 
CAGCACAACC TGGCTGTCCA CTTTTTTTGT CTGCTGATGT CTAGTGGAAT ATGCCAGAGA 
GGAGAGGAAG ATGAAGACGT GTATGGTTTT CTTGTGGGTG ACATTAAAAA GGAGATCCGC 
AGATCATCGA GGCTGAGATG CTTTCATTGC AAAAAGGCTG GTGCTTCAGT GGGCTGCTCC 
ATCAAAAGCT GTCGGCAAAT GGTCCACATG CCCTGTGGAT TAGAGCAGGA GTTTGTTTTC 
CAGTTCACAG GCTTGTTTCC GTCTTTCTGT AAAAAGCATG CGCCTACTCA GTCCTGTGAC 
TCCAGCCACA GTCTGCCCCA CTCCTGCTCC ATCTGTCTGG ATCCCATTGA ACCCATCCTC 
TCCTATCATG TCCTCAAATG CCCTGCATGT CATGGAAGCT GGTTTCACAG GAAATGTGTA 
CAGAATTATG CACACAGTGC CGCAATGTTT TTCTTTAAAT GCACACTCTG CAATAACAAG 
GAGCAGTTTC AGCAGGAAAT GCTCAGAATG GGAATATATA TACCAGAGCG TGATGCATCT 
TGGGAACTGG AGGAGAATGC ATTTGTAGAG TTATTGCAGG TCTACCACCG CTGTGATGCA 
GTGAAGTGTC TGTGTCACAA AGGTCGTGAA CACAGCTCAC AGTCAGGGTA TTTTGAAATA 
GTTCTCTGCA AGTTGTGTGG ATCCAGGGGA ACTCATCGAA AGTGCTCTAA TATTCGAGTT 
TATGAAGCTG ACTGGATGTG TGCTGACTGT AAAACTGCAG CTGAGGGCAA AACATCATTG 
CCATTGCCAA ATCATGTTGA ATCGCCACTG GCTATAAGAC AAGAGAGGAA GAGGCTGTTT 
AAATCGATCT CTGACATTCA CTCTTCAATC GTCGCTAAAA GGCAGTGTGT GCCAGCCACT 
TCTCCGGAGG TCTTAATGGA TCTGGCCTGT CAGATCTCAC AGCAGCAGTT CACAGAGGTG 
CTGGTGGGAG ATGATGATGG TGGCGTGATG GAAGCAGCTC TGCAGGTGCT GCGCCAAAGT 
GACTTTAATC CCTGCTGCAC ACTTTCTGTC AAATTCTCCA AGGACAAACT GAACAACAAC 
ATTAGAAACC AGCGTCGCTT CCTCAGGCGT CTCGTTTCAA AACTGCAGGT CTCAGACATT 
TTTGAGGGAT CCGATGGAGC TAAAAACCTG GCTCTGAACT CAAAAGCTCT GAGGGACGAT 
CTCTACTTTG ATGTCGGCAG TCTATTGGCC CTCTCTTTGG TTCACGGAGG TCCTCCTCTG 
GGCTTCTTCT CTCCTGCACT GTATCATAGT CTGTTCAACT ATCCCAAAAA CTACAGGCCT 
ACTTTAGAAG ACCTCGGAGA CACTGCTTTT GGGCACAAAA TCAGACAAAT CGCAGAAGCA 
AAGTCAATGA AGGAGCTGAG ACATGCGATG AAGTCAGCAT CACAATATTT GGAAGCTGCA 
GGCTGTTGGC GGAAGATCAG CAAACTCTCT GAGAAAGACA TGTTGGTGGA GGATGTACTG 
AATTTCTACC TGATCATCAG ACTGCAGCTT CCTCTGCAAA GATTCCGTGA AGGTTTGAGG 
ACACTAGGAA TGTTCGAGCA GGTCCAGATG TGCTCAGAGA CGTTCTTTCC AGTCTTCTGT 
GGACCTGTGG AAAGGCTGAC AGCTGAATCT GTCATGGAGC TCTTCACAAC CCGGTTATCA 
GAAGAAAAAG AAAAACAAGT TCTAGAAAAG ACCACGATAA GCTTTTGGAA ACAGTATCTG 
CATGAATGTG AAGAGGGTCA ATGTGCAGCA TCGCTGGAGG ACCTCCTAAC ATTTGCCACT 
GGTACAGATT TGGTTCCAGC CATCGGATTC AAGCCCACTC CGTCCATCTC ATTCCTCAGC 
TCACCAGACA ACTTGTGTGC TTTTCCTCAG AGCAACTGTG ATGCCAATCA TCTCATTCTG 
CCTACTCTAC CCTCTTACCA GCTCTTCAAG AAACATTTGG ATTACACAGT GTGTCAGTTC 
TCAGTTATGC AGGACATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

g2e3 ( XM_021467121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322796.1 Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149517
Clone ID Related Accession (Same CDS sequence)	XM_021467121.1
Accession Version	XM_021467121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	G2/M phase-specific E3 ubiquitin-protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGTG GAATATGCCA GAGAGGAGAG GAAGATGAAG ACGTGTATGG TTTTCTTGTG 
GGTGACATTA AAAAGGAGAT CCGCAGATCA TCGAGGCTGA GATGCTTTCA TTGCAAAAAG 
GCTGGTGCTT CAGTGGGCTG CTCCATCAAA AGCTGTCGGC AAATGGTCCA CATGCCCTGT 
GGATTAGAGC AGGAGTTTGT TTTCCAGTTC ACAGGCTTGT TTCCGTCTTT CTGTAAAAAG 
CATGCGCCTA CTCAGTCCTG TGACTCCAGC CACAGTCTGC CCCACTCCTG CTCCATCTGT 
CTAGATCCCA TTGAACCCAT CCTCTCCTAT CATGTCCTCA AATGCCCTGC ATGTCATGGA 
AGCTGGTTTC ACAGGAAATG TGTACAGAAT TATGCACACA GCGCGGCAAT GTTTTTCTTT 
AAATGCACAC TCTGCAATAA CAAGGAGCAG TTTCAGCAGG AAATGCTCCG AATGGGAATA 
TATATACCAG AGCGTGATGC ATCTTGGGAA CTGGAGGAGA ATGCATTTGT AGAGTTATTG 
CAGGTCTACC ACCGCTGTGA TGCAGTGAAG TGTCTGTGTC ACAAAGGTCG TGAACACAGC 
TCACAGTCAG GGTATTTTGA AATAGTTCTC TGCAAGTTGT GTGGATCCAG GGGAACTCAT 
CGAAAGTGCT CTAATATTCG AGTTTATGAA GCTGACTGGA TGTGTGCTGA CTGTAAAACT 
GCAGCTGAGG GCAAAACATC ATTGCCATTG CCAAATCATG TTGAATCGCC ACTGGCTATA 
AGACAAGAGA GGAAGAGGCT GTTTAAATCG ATCTCTGACA TTCACTCTTC AATCGTCGCT 
AAAAGGCAGT GTGTGCCAGC CACTTCTCCG GAGGTCTTAA TGGATCTGGC CTGTCAGATC 
TCACAGCAGC AGTTCACAGA GGTGCTGGTG GGAGATGATG ATGGTGGCGT GATGGAAGCA 
GCTCTGCAGG TGCTGCGCCA AAGTGACTTT AATCCCTGCT GCACACTTTC TGTCAAATTC 
TCCAAGGACA AACTGAACAA CAACATTAGA AACCAGCGTC GCTTCCTCAG GCGTCTCGTT 
TCAAAACTGC AGGTCTCAGA CATTTTTGAG GGATCCGATG GAGCTAAAAA CCTGGCTCTG 
AACTCAAAAG CTCTGAGGGA CGATCTCTAC TTTGATGTCG GCAGTCTATT GGCCCTCTCT 
TTGGTTCACG GAGGTCCTCC TCTGGGCTTC TTCTCTCCTG CACTGTATCA TAGTCTGTTC 
AACTATCCCA AAAACTACAG GCCTACTTTA GAAGACCTCG GAGACACTGC TTTTGGGCAC 
AAAATCAGAC AAATCGCAGA AGCAAAGTCA ATGAAGGAGC TGAGACATGC GATGAAGTCA 
GCATCACAAT ATTTGGAAGC TGCAGGCTGT TGGCGGAAGA TCAGCAAACT CTCTGAGAAA 
GACATGTTGG TGGAGGATGT ACTGAATTTC TACCTGATCA TCAGACTGCA GCTTCCTCTG 
CAAAGATTCC GTGAAGGTTT GAGGACACTA GGAATGTTCG AGCAGGTCCA GATGTGCTCA 
GAGACGTTCT TTCCAGTCTT CTGTGGACCT GTGGAAAGGC TGACGGCTGA ATCTGTCATG 
GAGCTCTTCA CAACCCGGTT ATCAGAAGAA AAAGAAAAAC AAGTTCTAGA AAAGACCACG 
ATAAGCTTTT GGAAACAGTA TCTGCATGAA TGTGAAGAGG GTCAATGTGC AGCATCGCTG 
GAGGACCTCC TAACATTTGC CACTGGTACA GATTTGGTTC CAGCCATCGG ATTCAAGCCC 
ACTCCGTCCA TCTCATTCCT CAGCTCACCA GACAACTTGT GTGCTTTTCC TCAGAGCAAC 
TGTGATGCCA ATCATCTCAT TCTGCCTACT CTACCCTCTT ACCAGCTCTT CAAGAAACAT 
TTGGATTACA CAGTGTGTCA GTTCTCAGTT ATGCAGGACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322796.1
CDS	351..2315
Translation	MSSGICQRGEEDEDVYGFLVGDIKKEIRRSSRLRCFHCKKAGASVGCSIKSCRQMVHMPCGLEQEFVFQFTGLFPSFCKKHAPTQSCDSSHSLPHSCSICLDPIEPILSYHVLKCPACHGSWFHRKCVQNYAHSAAMFFFKCTLCNNKEQFQQEMLRMGIYIPERDASWELEENAFVELLQVYHRCDAVKCLCHKGREHSSQSGYFEIVLCKLCGSRGTHRKCSNIRVYEADWMCADCKTAAEGKTSLPLPNHVESPLAIRQERKRLFKSISDIHSSIVAKRQCVPATSPEVLMDLACQISQQQFTEVLVGDDDGGVMEAALQVLRQSDFNPCCTLSVKFSKDKLNNNIRNQRRFLRRLVSKLQVSDIFEGSDGAKNLALNSKALRDDLYFDVGSLLALSLVHGGPPLGFFSPALYHSLFNYPKNYRPTLEDLGDTAFGHKIRQIAEAKSMKELRHAMKSASQYLEAAGCWRKISKLSEKDMLVEDVLNFYLIIRLQLPLQRFREGLRTLGMFEQVQMCSETFFPVFCGPVERLTAESVMELFTTRLSEEKEKQVLEKTTISFWKQYLHECEEGQCAASLEDLLTFATGTDLVPAIGFKPTPSISFLSSPDNLCAFPQSNCDANHLILPTLPSYQLFKKHLDYTVCQFSVMQDI

Target ORF information:

RefSeq Version	XM_021467121.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G2/M-phase specific E3 ubiquitin protein ligase (g2e3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467121.1

ORF Insert Sequence:

ATGTCTAGTG GAATATGCCA GAGAGGAGAG GAAGATGAAG ACGTGTATGG TTTTCTTGTG 
GGTGACATTA AAAAGGAGAT CCGCAGATCA TCGAGGCTGA GATGCTTTCA TTGCAAAAAG 
GCTGGTGCTT CAGTGGGCTG CTCCATCAAA AGCTGTCGGC AAATGGTCCA CATGCCCTGT 
GGATTAGAGC AGGAGTTTGT TTTCCAGTTC ACAGGCTTGT TTCCGTCTTT CTGTAAAAAG 
CATGCGCCTA CTCAGTCCTG TGACTCCAGC CACAGTCTGC CCCACTCCTG CTCCATCTGT 
CTAGATCCCA TTGAACCCAT CCTCTCCTAT CATGTCCTCA AATGCCCTGC ATGTCATGGA 
AGCTGGTTTC ACAGGAAATG TGTACAGAAT TATGCACACA GCGCGGCAAT GTTTTTCTTT 
AAATGCACAC TCTGCAATAA CAAGGAGCAG TTTCAGCAGG AAATGCTCCG AATGGGAATA 
TATATACCAG AGCGTGATGC ATCTTGGGAA CTGGAGGAGA ATGCATTTGT AGAGTTATTG 
CAGGTCTACC ACCGCTGTGA TGCAGTGAAG TGTCTGTGTC ACAAAGGTCG TGAACACAGC 
TCACAGTCAG GGTATTTTGA AATAGTTCTC TGCAAGTTGT GTGGATCCAG GGGAACTCAT 
CGAAAGTGCT CTAATATTCG AGTTTATGAA GCTGACTGGA TGTGTGCTGA CTGTAAAACT 
GCAGCTGAGG GCAAAACATC ATTGCCATTG CCAAATCATG TTGAATCGCC ACTGGCTATA 
AGACAAGAGA GGAAGAGGCT GTTTAAATCG ATCTCTGACA TTCACTCTTC AATCGTCGCT 
AAAAGGCAGT GTGTGCCAGC CACTTCTCCG GAGGTCTTAA TGGATCTGGC CTGTCAGATC 
TCACAGCAGC AGTTCACAGA GGTGCTGGTG GGAGATGATG ATGGTGGCGT GATGGAAGCA 
GCTCTGCAGG TGCTGCGCCA AAGTGACTTT AATCCCTGCT GCACACTTTC TGTCAAATTC 
TCCAAGGACA AACTGAACAA CAACATTAGA AACCAGCGTC GCTTCCTCAG GCGTCTCGTT 
TCAAAACTGC AGGTCTCAGA CATTTTTGAG GGATCCGATG GAGCTAAAAA CCTGGCTCTG 
AACTCAAAAG CTCTGAGGGA CGATCTCTAC TTTGATGTCG GCAGTCTATT GGCCCTCTCT 
TTGGTTCACG GAGGTCCTCC TCTGGGCTTC TTCTCTCCTG CACTGTATCA TAGTCTGTTC 
AACTATCCCA AAAACTACAG GCCTACTTTA GAAGACCTCG GAGACACTGC TTTTGGGCAC 
AAAATCAGAC AAATCGCAGA AGCAAAGTCA ATGAAGGAGC TGAGACATGC GATGAAGTCA 
GCATCACAAT ATTTGGAAGC TGCAGGCTGT TGGCGGAAGA TCAGCAAACT CTCTGAGAAA 
GACATGTTGG TGGAGGATGT ACTGAATTTC TACCTGATCA TCAGACTGCA GCTTCCTCTG 
CAAAGATTCC GTGAAGGTTT GAGGACACTA GGAATGTTCG AGCAGGTCCA GATGTGCTCA 
GAGACGTTCT TTCCAGTCTT CTGTGGACCT GTGGAAAGGC TGACGGCTGA ATCTGTCATG 
GAGCTCTTCA CAACCCGGTT ATCAGAAGAA AAAGAAAAAC AAGTTCTAGA AAAGACCACG 
ATAAGCTTTT GGAAACAGTA TCTGCATGAA TGTGAAGAGG GTCAATGTGC AGCATCGCTG 
GAGGACCTCC TAACATTTGC CACTGGTACA GATTTGGTTC CAGCCATCGG ATTCAAGCCC 
ACTCCGTCCA TCTCATTCCT CAGCTCACCA GACAACTTGT GTGCTTTTCC TCAGAGCAAC 
TGTGATGCCA ATCATCTCAT TCTGCCTACT CTACCCTCTT ACCAGCTCTT CAAGAAACAT 
TTGGATTACA CAGTGTGTCA GTTCTCAGTT ATGCAGGACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

A subtracted cDNA library from the zebrafish (Danio rerio) embryonic inner ear.
Genome research12(6)1007-11(2002 Jun)
Coimbra RS,Weil D,Brottier P,Blanchard S,Levi M,Hardelin JP,Weissenbach J,Petit C

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g2e3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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