heatr5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	heatr5a
Entrez Gene ID	368728
Full Name	HEAT repeat containing 5a
Synonyms	CG2747,si:busm1-142b24.3,si:dz142b24.3,zgc:63665
General protein information	Preferred Names HEAT repeat containing 5a Names HEAT repeat-containing protein 5A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200478.2	NP_956772.2	HEAT repeat-containing protein 5A
XM_009293121.1	XP_009291396.1	HEAT repeat-containing protein 5A isoform X1
XM_009293122.1	XP_009291397.1	HEAT repeat-containing protein 5A isoform X1
XM_009293123.1	XP_009291398.1	HEAT repeat-containing protein 5A isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HEATR5A	XP_005222124.1
Mus musculus (house mouse)	Heatr5a	NP_796145.2
Gallus gallus (chicken)	HEATR5A	XP_004941794.1
Homo sapiens (human)	HEATR5A	NP_056288.2
Danio rerio (zebrafish)	heatr5a	NP_956772.2
Canis lupus familiaris (dog)	HEATR5A	XP_005623357.1
Pan troglodytes (chimpanzee)	HEATR5A	XP_522993.3
	LOC716984	XP_001108090.2
Rattus norvegicus (Norway rat)	Heatr5a	XP_006240152.1
Xenopus tropicalis (tropical clawed frog)	heatr5a	NP_001090667.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following heatr5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the heatr5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01840	NM_200478.2 Latest version!	Danio rerio HEAT repeat containing 5a (heatr5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40238	XM_009293121.1 Latest version!	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40238	XM_009293122.1 Latest version!	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40239	XM_009293123.1 Latest version!	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

heatr5a ( NM_200478.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956772.2 Danio rerio HEAT repeat containing 5a (heatr5a), mRNA.

Price & Availability↑

CloneID	ODa01840
Clone ID Related Accession (Same CDS sequence)	NM_200478.2
Accession Version	NM_200478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Danio rerio(zebrafish)
Product	HEAT repeat-containing protein 5A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL593845.5. On Jul 30, 2004 this sequence version replaced NM_200478.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA TAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
CTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAGACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG CGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTACTCGGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTGCTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATAAAGAGT 
CTGGTGGTTC ATTGCAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTGA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTGG CTCTGCTGGG TCCCGGACTC CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AAGAGGTCCT GGAGCAGTTT TGTGAATTTG TGCGACAGCT TAAAGGAGCT 
CGGCAGCAAA CAGTCCAAAT ACACGTAACT GCTGCTTTCT TTTGCACTTT GAAGAGTTTG 
GCATCAAGTC GGAGGGAATT GGGGCCAGAG GAGGTCCAAA GACCAGCATT GTCTCTCCTG 
TTGGGGGCGT TGGAGGGCTC AAACCCTCTA CTGCGCTGCA TGGCTGCAGA GGGTTTAGCA 
AGGCTGGTGC AAGTTCTTAT TGACCCCGGC TTCACAGTTT CCCTCACTCT CATGAGCTTT 
GACAAACTGA AGACAGCAAG GGATGCCATC ACACGCACAG GCCATGCCTT GGCCCTCGGA 
ACGGTTTACC GTTATCTTGG TGGGATTAGC TCCCCTCAGT ACCTCAGTGC CTGTGTTGGG 
GTTCTTTTTA CCCTGTCCCA GGATAATACC TCACCAGAAG TACAGATGTG GGCTCTTCAT 
GCTCTGTCCA CTGTGGTGGA CCTAGCAGGT CCTTTGTACC ACAGTCACCT GGAGGCCAGC 
TTCACTCTGG TGCTGCGTTT GCTCCTCTCT ACCCCACACA CACACGTGGA GGTGCAGCAA 
AGCCTGGGCC GCTGTCTTAA CGCCCTCATC ACCTCTGTGG GGCCGGATCT GCAAGGTGAG 
GGTCCAGGTG TGTGTGCGGT GAGGACCTCG TGTTTGGTGG GCTGTGCTGT GATGCAGGAT 
AGCCAGGACT GTTTAGTCCA GGCTCTGGCC ATCTCTTGCC TCCAGCAGCT GCACATGTTT 
GCGCCACGCT TCGTCAATCT GTCCAGCCTT GTGCCAAGTC TCTGTATAAA TCTGTGCAGC 
TCATACCTGT CTCTGCGGCG AGCGGTTGTG GCCTGTTTAA GACAGCTGGC TCAGAGAGAG 
GCAGTGGAGG TATCGGAACA CGCAGTGGCT CTGGTTAAAG AGCTTCCCCG CAGAGACAAC 
ACACAGCTGG ATGTCACTAT TAAGGAGGTG GGCTTGGAGG GAGCTTTGTT CAGTCTCCTG 
GACAGGGAGT CGGATCCCCG CCTGTGCAGA GACATTCAGG AAACACTGGT CCATATGATG 
AGTTCAGCAG CTGAGAGTAA GCTGGCCCAC TGGCTGAAGC TCTGCAAAGA TGTACTGTCC 
GCTTCTGCAG ACTCCGCTGC AGCCGCCTCT GTAGAAACCC AGCAAGAGGA GGATGGAGAT 
CGATATGATG ACTCCTCTGC ATTCCATGCA AAATCTGAGT CCAGCGGGCC CTTCAATAAC 
CTGCGCTGGT CCACTCGTGT GTTTTCTATG GAGTGTGTGT GTCGCATAAT AGCACAGTGT 
GAGAACAGAG ACTCAGCTCA CTTTAACATG GCACTTGCTC AAGAGCAGCG TTTACATGAG 
TCCACAGATT TCCTGGTTCT TCACTTGGCG GATCTGATCC GCATGGCATT CATGGCATCC 
ACAGACCACA GTGACCAGCT CCGACTGGCT GGTTTGCAAA CGCTTCTGGT CATTATTCGT 
AAATTCTCCA ATGTACCAGA GCCTGAGTTC CCTGGGCATG TCATTCTGGA GCAGTATCAA 
GCCAATGTTG GAGCTGCGCT TAGACCTGCC TTCCATGTGG ACGCTCCTCC AAACGTGACC 
GCCAAAGCTT GCCAGGTCTG CAGTGCATGG ATCGCCAGTG GGGTCATCAG TGACCTGCGG 
GACCTGAGAA GGGTACATCA ACTTTTGGCA TCCTCTCTGG CCAAAGTGCA GGTGGGGAGA 
GATGTTCCCA GTCAGCTCTA TAATGAAAGC ACCTTCACCA TGGAGTCGCT GGCGGTGCTC 
AAAGCCTGGG CGGAGGTCTA CATCACAGCA GTGCAGGGCT CTAGGCAGAG GGACAGTCCG 
GTCAGTCATC AGCAGCAGCA GAGTGAGGAG GCTGGGACTG CAGGTCAAGC AGGGGCTGGT 
TTGCTCAAAC TAGTTCAGAC TGATCTGGCA ACCCTGAGTC GCCTATGGCT GGCAGCCCTT 
CAGGATCATG CCCTGCTCAC ACTGCCCCCA CACTACTCTA GTCATCTTCC CTCCACAGGC 
GGCTCATTTT ACACTGCAGA AACGGTGGAA CAGGCCCGTC CACATTACTA CAGTGCCTGG 
GCAACCATTC TCCATGCCAC TGCCCTCTGG CTCAACAGCA CCGGCTTTAT CGTGGTGGAT 
GAGGGCCCTG CAAACCTTTC CAGGCCCGTC ACGCCCACTT CCATGGGCCA GTCCACATCA 
CTGAGCAACG TTAAATCTCC AGAGGACATC AACAGTGATC GCCTACACCT CATCCTTGGG 
ATCAGTGTGG AGTTTTTGTG CTCTCCTCAT TCTGGAGACC AAATGGAAAA CATTCATTCA 
TGTCTTCAGG CTCTGCAGGC CTTATTGGAG GTGCCTTGGC CTCGCTCAAA AGTGGGCAAT 
GATCAGGCTC TGAGTGTGGA GCTGCTTAGC ATCCTGCATA GGCTCATAGT CACTCGGGAA 
TCTCCCAGCA TTCAGCTGGC TGTGCTGGAA CTGGTGCAGC AGATTATTTG TGCAGCTCAG 
GAACACGTCA GAGAAAAACG CCACAGCGCA GAGGTGGATG ACGGTGCGGC AGAGAAGGAA 
ACAGTTCCTG AGTTTGGTGA GGGACGAGAC ACTGGTGGCC TGGTGCCAGG GAGATCTCTC 
GTGTTTGGAG CACTGGAGCT GTGTCTCTGC ACGCTTGTGA GGAAGCTTCC GCAGCTCAGC 
CCCAAACTGA CCGGCAGTCC CACGGGACGG GGAGGACAGA CGTGCTCCCT CAGCGCCACT 
GACTGCAGAC TCGTCACATC TGCTCTGGCC ATCCTCTCTG AACTGCCCTC CATCTGTTCA 
CCAGAGGGCA GTGTGTCAGT GCTGCCCACA GTGCTGTATC TGCTGCTCGG GGTTTTGAAA 
GAGGCGGTGA AAGGCTCTGT TGGAACTGAG AGTGGACAGC TGGTGTCAGG CATCCTGCAG 
GCCCTGCGCA CTCTTCTCAC TTCTCCCATG AGCAGAGCTG AGAAGAGTCG TGGGGCCTGG 
ACTCAGCTAC TGCAATGCGC ACTTCACACA CTCCTGGAGT CCTGGAGTAC AGATAAAGTG 
GAGCCTGGAG TAGATGAGGT CACCATGTTG ACATCGCTCA CCATCTTCCT GCTGTACGCC 
AGTGCTGAGG TCACCTCTGT AGAGCCACTG CAAACCCGCT GCATCCAGAC ATTCAGAGCA 
AGTCTGGACT CTAAAGACCC CGTGGTGCTG AGCAGGAGTT ACCAGTTGCT ATTATCTTTA 
TTTCAAGGCC CAGCACCAGT AGCAAGGCCA TTTATTCTGG CTCTAGGTGG TCGTCTGGTG 
TCTCAACTGG AGGAAGCTGA AAAGAGTCGT CCTCAAGGTC CAGCAGAGCT ACAGGCCGTA 
CAAGATGCAA TCCGGGCCCT GGAAGCACTA GTGTTTGCTG CGGATGAAAC TCACCGTCCT 
CAGCTGGTGG CTGTGCTGCT GCCTATCCTC ATCTCCCTCC TCTTAGATGA GAATGCTTTG 
GCATCGGCTC CGGCGGCTTC TCGCTCGCTG CACGAATCTG CCTTGAGAGA CCTCATGCGC 
ATCGGCCCCC AGCACTCGGC TGTCTTAAAA GCGCTGATGG CCTCCGCTCC GCACATGAAA 
GCCAGACTGG AAGCAGCAGT CAAGGGGAAC CAGGAGAGCG TGAACTCTAA AGCACAAGCA 
CCTCCAGCCA TCAGCAAAAA CACACCCAGC ATTCAACTGA AGATCAATTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956772.2
CDS	1..5997
Translation	MEQAHSLLLNEEACSQLPEHQRAEFVFEWLRFLKKLLPATDRADVKQKQKRLVEQLTTVLTSSPGPPTRLLLAQCLALVYRVGDSLTSSLTVDRCNDIIRSKDDSPSFLPTRLAAVACLGALYEQLGRLLINSFKETTTNLLKAMRSAESQGRCEIMLCVERILRGLGVSAVSCHRDIYKAARTGLTDRSMAVRCAAAKCLLELQREAVFLWSTELDNVATLCFRALEGSNYDVRVSISKLLGTLLASALEPRQAIAPRPGSKRSSLEEVMELLSSGFLRGGAGFLRASGDMLKGTSSVSRDVRVGITQTCVVFVSILGGVWLETHFSRLLSLLMEWASHTRATQYPADAVSCRCCVSFILRATLGTLLGEKAQIAAAKEICQVISKQKRVVDASLHEGNMETRVSPADVAASQHVLVCALLELGSLVQDLSSTAAPLLQDTGIGMLDTVISVLLHPSASARLASAWCLRCIAVGMPAQVAVLLDRCVERLNALKSCPEAVAGYSAAIAALLGAVQLSPLGISHSKGKMVMTLAEDLLRSAAQNSRISIQRTQGGWLLLGALSTLGPTVMEHHLPRLLLLWKCAFPLSVKDVEMELRRGDSFTWQVTLEGRAGALCAIKSLVVHCKELLTDDVICRFVPLLSCAVALLTQLPSIIKSYGNQIKNAATVFKLRLYEILKLLQPKIYEESFGTVLKQLLNDLTGPEITACAERNLLPSLCYSQDLALLGPGLQDMDQRYIEEQLHGGGSGGGTLEYDSFTIFEKSQEVLEQFCEFVRQLKGARQQTVQIHVTAAFFCTLKSLASSRRELGPEEVQRPALSLLLGALEGSNPLLRCMAAEGLARLVQVLIDPGFTVSLTLMSFDKLKTARDAITRTGHALALGTVYRYLGGISSPQYLSACVGVLFTLSQDNTSPEVQMWALHALSTVVDLAGPLYHSHLEASFTLVLRLLLSTPHTHVEVQQSLGRCLNALITSVGPDLQGEGPGVCAVRTSCLVGCAVMQDSQDCLVQALAISCLQQLHMFAPRFVNLSSLVPSLCINLCSSYLSLRRAVVACLRQLAQREAVEVSEHAVALVKELPRRDNTQLDVTIKEVGLEGALFSLLDRESDPRLCRDIQETLVHMMSSAAESKLAHWLKLCKDVLSASADSAAAASVETQQEEDGDRYDDSSAFHAKSESSGPFNNLRWSTRVFSMECVCRIIAQCENRDSAHFNMALAQEQRLHESTDFLVLHLADLIRMAFMASTDHSDQLRLAGLQTLLVIIRKFSNVPEPEFPGHVILEQYQANVGAALRPAFHVDAPPNVTAKACQVCSAWIASGVISDLRDLRRVHQLLASSLAKVQVGRDVPSQLYNESTFTMESLAVLKAWAEVYITAVQGSRQRDSPVSHQQQQSEEAGTAGQAGAGLLKLVQTDLATLSRLWLAALQDHALLTLPPHYSSHLPSTGGSFYTAETVEQARPHYYSAWATILHATALWLNSTGFIVVDEGPANLSRPVTPTSMGQSTSLSNVKSPEDINSDRLHLILGISVEFLCSPHSGDQMENIHSCLQALQALLEVPWPRSKVGNDQALSVELLSILHRLIVTRESPSIQLAVLELVQQIICAAQEHVREKRHSAEVDDGAAEKETVPEFGEGRDTGGLVPGRSLVFGALELCLCTLVRKLPQLSPKLTGSPTGRGGQTCSLSATDCRLVTSALAILSELPSICSPEGSVSVLPTVLYLLLGVLKEAVKGSVGTESGQLVSGILQALRTLLTSPMSRAEKSRGAWTQLLQCALHTLLESWSTDKVEPGVDEVTMLTSLTIFLLYASAEVTSVEPLQTRCIQTFRASLDSKDPVVLSRSYQLLLSLFQGPAPVARPFILALGGRLVSQLEEAEKSRPQGPAELQAVQDAIRALEALVFAADETHRPQLVAVLLPILISLLLDENALASAPAASRSLHESALRDLMRIGPQHSAVLKALMASAPHMKARLEAAVKGNQESVNSKAQAPPAISKNTPSIQLKINFL

Target ORF information:

RefSeq Version	NM_200478.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HEAT repeat containing 5a (heatr5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200478.2

ORF Insert Sequence:

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA TAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
CTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAGACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG CGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTACTCGGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTGCTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATAAAGAGT 
CTGGTGGTTC ATTGCAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTGA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTGG CTCTGCTGGG TCCCGGACTC CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AAGAGGTCCT GGAGCAGTTT TGTGAATTTG TGCGACAGCT TAAAGGAGCT 
CGGCAGCAAA CAGTCCAAAT ACACGTAACT GCTGCTTTCT TTTGCACTTT GAAGAGTTTG 
GCATCAAGTC GGAGGGAATT GGGGCCAGAG GAGGTCCAAA GACCAGCATT GTCTCTCCTG 
TTGGGGGCGT TGGAGGGCTC AAACCCTCTA CTGCGCTGCA TGGCTGCAGA GGGTTTAGCA 
AGGCTGGTGC AAGTTCTTAT TGACCCCGGC TTCACAGTTT CCCTCACTCT CATGAGCTTT 
GACAAACTGA AGACAGCAAG GGATGCCATC ACACGCACAG GCCATGCCTT GGCCCTCGGA 
ACGGTTTACC GTTATCTTGG TGGGATTAGC TCCCCTCAGT ACCTCAGTGC CTGTGTTGGG 
GTTCTTTTTA CCCTGTCCCA GGATAATACC TCACCAGAAG TACAGATGTG GGCTCTTCAT 
GCTCTGTCCA CTGTGGTGGA CCTAGCAGGT CCTTTGTACC ACAGTCACCT GGAGGCCAGC 
TTCACTCTGG TGCTGCGTTT GCTCCTCTCT ACCCCACACA CACACGTGGA GGTGCAGCAA 
AGCCTGGGCC GCTGTCTTAA CGCCCTCATC ACCTCTGTGG GGCCGGATCT GCAAGGTGAG 
GGTCCAGGTG TGTGTGCGGT GAGGACCTCG TGTTTGGTGG GCTGTGCTGT GATGCAGGAT 
AGCCAGGACT GTTTAGTCCA GGCTCTGGCC ATCTCTTGCC TCCAGCAGCT GCACATGTTT 
GCGCCACGCT TCGTCAATCT GTCCAGCCTT GTGCCAAGTC TCTGTATAAA TCTGTGCAGC 
TCATACCTGT CTCTGCGGCG AGCGGTTGTG GCCTGTTTAA GACAGCTGGC TCAGAGAGAG 
GCAGTGGAGG TATCGGAACA CGCAGTGGCT CTGGTTAAAG AGCTTCCCCG CAGAGACAAC 
ACACAGCTGG ATGTCACTAT TAAGGAGGTG GGCTTGGAGG GAGCTTTGTT CAGTCTCCTG 
GACAGGGAGT CGGATCCCCG CCTGTGCAGA GACATTCAGG AAACACTGGT CCATATGATG 
AGTTCAGCAG CTGAGAGTAA GCTGGCCCAC TGGCTGAAGC TCTGCAAAGA TGTACTGTCC 
GCTTCTGCAG ACTCCGCTGC AGCCGCCTCT GTAGAAACCC AGCAAGAGGA GGATGGAGAT 
CGATATGATG ACTCCTCTGC ATTCCATGCA AAATCTGAGT CCAGCGGGCC CTTCAATAAC 
CTGCGCTGGT CCACTCGTGT GTTTTCTATG GAGTGTGTGT GTCGCATAAT AGCACAGTGT 
GAGAACAGAG ACTCAGCTCA CTTTAACATG GCACTTGCTC AAGAGCAGCG TTTACATGAG 
TCCACAGATT TCCTGGTTCT TCACTTGGCG GATCTGATCC GCATGGCATT CATGGCATCC 
ACAGACCACA GTGACCAGCT CCGACTGGCT GGTTTGCAAA CGCTTCTGGT CATTATTCGT 
AAATTCTCCA ATGTACCAGA GCCTGAGTTC CCTGGGCATG TCATTCTGGA GCAGTATCAA 
GCCAATGTTG GAGCTGCGCT TAGACCTGCC TTCCATGTGG ACGCTCCTCC AAACGTGACC 
GCCAAAGCTT GCCAGGTCTG CAGTGCATGG ATCGCCAGTG GGGTCATCAG TGACCTGCGG 
GACCTGAGAA GGGTACATCA ACTTTTGGCA TCCTCTCTGG CCAAAGTGCA GGTGGGGAGA 
GATGTTCCCA GTCAGCTCTA TAATGAAAGC ACCTTCACCA TGGAGTCGCT GGCGGTGCTC 
AAAGCCTGGG CGGAGGTCTA CATCACAGCA GTGCAGGGCT CTAGGCAGAG GGACAGTCCG 
GTCAGTCATC AGCAGCAGCA GAGTGAGGAG GCTGGGACTG CAGGTCAAGC AGGGGCTGGT 
TTGCTCAAAC TAGTTCAGAC TGATCTGGCA ACCCTGAGTC GCCTATGGCT GGCAGCCCTT 
CAGGATCATG CCCTGCTCAC ACTGCCCCCA CACTACTCTA GTCATCTTCC CTCCACAGGC 
GGCTCATTTT ACACTGCAGA AACGGTGGAA CAGGCCCGTC CACATTACTA CAGTGCCTGG 
GCAACCATTC TCCATGCCAC TGCCCTCTGG CTCAACAGCA CCGGCTTTAT CGTGGTGGAT 
GAGGGCCCTG CAAACCTTTC CAGGCCCGTC ACGCCCACTT CCATGGGCCA GTCCACATCA 
CTGAGCAACG TTAAATCTCC AGAGGACATC AACAGTGATC GCCTACACCT CATCCTTGGG 
ATCAGTGTGG AGTTTTTGTG CTCTCCTCAT TCTGGAGACC AAATGGAAAA CATTCATTCA 
TGTCTTCAGG CTCTGCAGGC CTTATTGGAG GTGCCTTGGC CTCGCTCAAA AGTGGGCAAT 
GATCAGGCTC TGAGTGTGGA GCTGCTTAGC ATCCTGCATA GGCTCATAGT CACTCGGGAA 
TCTCCCAGCA TTCAGCTGGC TGTGCTGGAA CTGGTGCAGC AGATTATTTG TGCAGCTCAG 
GAACACGTCA GAGAAAAACG CCACAGCGCA GAGGTGGATG ACGGTGCGGC AGAGAAGGAA 
ACAGTTCCTG AGTTTGGTGA GGGACGAGAC ACTGGTGGCC TGGTGCCAGG GAGATCTCTC 
GTGTTTGGAG CACTGGAGCT GTGTCTCTGC ACGCTTGTGA GGAAGCTTCC GCAGCTCAGC 
CCCAAACTGA CCGGCAGTCC CACGGGACGG GGAGGACAGA CGTGCTCCCT CAGCGCCACT 
GACTGCAGAC TCGTCACATC TGCTCTGGCC ATCCTCTCTG AACTGCCCTC CATCTGTTCA 
CCAGAGGGCA GTGTGTCAGT GCTGCCCACA GTGCTGTATC TGCTGCTCGG GGTTTTGAAA 
GAGGCGGTGA AAGGCTCTGT TGGAACTGAG AGTGGACAGC TGGTGTCAGG CATCCTGCAG 
GCCCTGCGCA CTCTTCTCAC TTCTCCCATG AGCAGAGCTG AGAAGAGTCG TGGGGCCTGG 
ACTCAGCTAC TGCAATGCGC ACTTCACACA CTCCTGGAGT CCTGGAGTAC AGATAAAGTG 
GAGCCTGGAG TAGATGAGGT CACCATGTTG ACATCGCTCA CCATCTTCCT GCTGTACGCC 
AGTGCTGAGG TCACCTCTGT AGAGCCACTG CAAACCCGCT GCATCCAGAC ATTCAGAGCA 
AGTCTGGACT CTAAAGACCC CGTGGTGCTG AGCAGGAGTT ACCAGTTGCT ATTATCTTTA 
TTTCAAGGCC CAGCACCAGT AGCAAGGCCA TTTATTCTGG CTCTAGGTGG TCGTCTGGTG 
TCTCAACTGG AGGAAGCTGA AAAGAGTCGT CCTCAAGGTC CAGCAGAGCT ACAGGCCGTA 
CAAGATGCAA TCCGGGCCCT GGAAGCACTA GTGTTTGCTG CGGATGAAAC TCACCGTCCT 
CAGCTGGTGG CTGTGCTGCT GCCTATCCTC ATCTCCCTCC TCTTAGATGA GAATGCTTTG 
GCATCGGCTC CGGCGGCTTC TCGCTCGCTG CACGAATCTG CCTTGAGAGA CCTCATGCGC 
ATCGGCCCCC AGCACTCGGC TGTCTTAAAA GCGCTGATGG CCTCCGCTCC GCACATGAAA 
GCCAGACTGG AAGCAGCAGT CAAGGGGAAC CAGGAGAGCG TGAACTCTAA AGCACAAGCA 
CCTCCAGCCA TCAGCAAAAA CACACCCAGC ATTCAACTGA AGATCAATTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

heatr5a ( XM_009293121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291396.1 Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X1, mRNA.

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CloneID	ODa40238
Clone ID Related Accession (Same CDS sequence)	XM_009293121.1 , XM_009293122.1
Accession Version	XM_009293121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTGAGATC TTGTTGGATT ATTCTGTGTT GATAAATCTG 
TGCAGCTCAT ACCTGTCTCT GCGGCGAGCG GTTGTGGCCT GTTTAAGACA GCTGGCTCAG 
AGAGAGGCAG TGGAGGTATC GGAACACGCA GTGGCTCTGG TTAAAGAGCT TCCCCGCAGA 
GACAACACAC AGCTGGATGT CACTATTAAG GAGGTGGGCT TGGAGGGAGC TTTGTTCAGT 
CTCCTGGACA GGGAGTCGGA TCCCCGCCTG TGCAGAGACA TTCAGGAAAC ACTGGTCCAT 
ATGATGAGTT CAGCAGCTGA GAGTAAGCTG GCCCACTGGC TGAAGCTCTG CAAAGATGTA 
CTGTCCGCTT CTGCAGACTC CGCTGCAGCC GCCTCTGTAG AAACCCAGCA AGAGGAGGAT 
GGAGATCGAT ATGATGACTC CTCTGCATTC CATGCAAAAT CTGAGTCCAG CGGGCCCTTC 
AATAACCTGC GCTGGTCCAC TCGTGTGTTT TCTATGGAGT GTGTGTGTCG CATAATAGCA 
CAGTGTGAGA ACAGAGACTC AGCTCACTTT AACATGGCAC TTGCTCAAGA GCAGCGTTTA 
CATGAGTCCA CAGATTTCCT GGTTCTTCAC TTGGCGGATC TGATCCGCAT GGCGTTCATG 
GCATCCACAG ACCACAGTGA CCAGCTCCGA CTGGCTGGTT TGCAAACGCT TCTGGTCATT 
ATTCGTAAAT TCTCCAATGT ACCAGAGCCT GAGTTCCCTG GGCATGTCAT TCTGGAGCAG 
TATCAAGCCA ATGTTGGAGC TGCGCTTAGA CCTGCCTTCC ATGTGGACGC TCCTCCAAAC 
GTGACCGCCA AAGCTTGCCA GGTCTGCAGT GCATGGATCG CCAGTGGGGT CATCAGTGAC 
CTGCGGGACC TGAGAAGGGT ACATCAACTT TTGGCATCCT CTCTGGCCAA AGTGCAGGTG 
GGGAGAGATG TTCCCAGTCA GCTCTATAAT GAAAGCACCT TCACCATGGA GTCGCTGGCG 
GTGCTCAAAG CCTGGGCGGA GGTCTACATC ACAGCAGTGC AGGGCTCTAG GCAGAGGGAC 
AGTCCGGTCA GTCATCAGCA GCAGCAGAGT GAGGAGGCTG GGACTGCAGG TCAAGCAGGG 
GCTGGTTTGC TCAAACTAGT TCAGACTGAT CTGGCAACCC TGAGTCGCCT ATGGCTGGCA 
GCCCTTCAGG ATCATGCCCT GCTCACACTG CCCCCACACT ACTCTAGTCA TCTTCCCTCC 
ACAGGCGGCT CATTTTACAC TGCAGAAACG GTGGAACAGG CCCGTCCACA TTACTACAGT 
GCCTGGGCAA CCATTCTCCA TGCCACTGCC CTCTGGCTCA ACAGCACCGG CTTTATCGTG 
GTGGATGAGG GCCCTGCAAA CCTTTCCAGG CCCGTCACGC CCACTTCCAT GGGCCAGTCC 
ACATCACTGA GCAACGTTAA ATCTCCAGAG GACATCAACA GTGATCGCCT ACACCTCATC 
CTTGGGATCA GTGTGGAGTT TTTGTGCTCT CCTCATTCTG GAGACCAAAT GGAAAACATT 
CATTCATGTC TTCAGGCTCT GCAGGCCTTA TTGGAGGTGC CTTGGCCTCG CTCAAAAGTG 
GGCAATGATC AGGCTCTGAG TGTGGAGCTG CTTAGCATCC TGCATAGGCT CATAGTCACT 
CGGGAATCTC CCAGCATTCA GCTGGCTGTG CTGGAACTGG TGCAGCAGAT TATTTGTGCA 
GCTCAGGAAC ACGTCAGAGA AAAACGCCAC AGCGCAGAGG TGGATGACGG TGCGGCAGAG 
AAGGAAACAG TTCCTGAGTT TGGTGAGGGA CGAGACACTG GTGGCCTGGT GCCAGGGAGA 
TCTCTCGTGT TTGGAGCACT GGAGCTGTGT CTCTGCACGC TTGTGAGGAA GCTTCCGCAG 
CTCAGCCCCA AACTGACCGG CAGTCCCACG GGACGGGGAG GACAGACGTG CTCCCTCAGC 
GCCACTGACT GCAGACTCGT CACATCTGCT CTGGCCATCC TCTCTGAACT GCCCTCCATC 
TGTTCACCAG AGGGCAGTGT GTCAGTGCTG CCCACAGTGC TGTATCTGCT GCTCGGGGTT 
TTGAAAGAGG CAGTGAAAGG CTCTGTTGGA ACTGAGAGTG GACAGCTGGT GTCAGGCATC 
CTGCAGGCCC TGCGCACTCT TCTCACTTCT CCCATGAGCA GAGCTGAGAA GAGTCGTGGG 
GCCTGGACTC AACTACTGCA ATGCGCACTT CACACACTCC TGGAGTCCTG GAGTACAGAT 
AAAGTGGAGC CTGGAGTAGA TGAGGTCACC ATGTTGACAT CGCTCACCAT CTTCCTGCTG 
TACGCCAGCG CTGAGGTCAC CTCTGTAGAG CCACTGCAAA CCCGCTGCAT CCAGACATTC 
AGAGCAAGTC TGGACTCCAA AGACCCCGTG GTGCTGAGCA GGAGTTACCA GTTGCTATTA 
TCTTTATTTC AAGGCCCAGC GCCAGTAGCA AGGCCATTTA TTCTGGCTCT AGGTGGTCGT 
CTGGTGTCTC AACTGGAGGA AGCTGAAAAG AGTCGTCCTC AAGGCCCAGC AGAGCTACAG 
GCCGTACAAG ATGCAATCCG GGCCCTGGAA GCACTAGTGT TTGCTGCGGA TGAAACTCAC 
CGTCCTCAGC TGGTGGCTGT GCTGCTGCCT ATCCTCATCT CCCTCCTCTT AGATGAGAAT 
GCTTTGGCAT CGGCTCCGGC GGCTTCTCGC TTGCTGCACG AATCTGCCTT GAGAGACCTC 
ATGCGCATCG GCCCCCAGCA CTCGGCTGTC TTAAAAGCGC TGATGGCCTC CGCTCCGCAC 
ATGAAAGCCA GACTGGAAGC AGCAGTCAAG GGGAACCAGG AGAGCGTGAA CTCTAAAGCA 
CAAGCACCTC CAGCCATCAG CAAAAACACA CCCAGCATTC AACTGAAGAT CAATTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291396.1
CDS	223..6345
Translation	MEQAHSLLLNEEACSQLPEHQRAEFVFEWLRFLKKLLPATDRADVKQKQKRLVEQLTTVLTSSPGPPTRLLLAQCLALVYRVGDSLTSSLTVDRCNDIIRSKDDSPSFLPTRLAAVACLGALYEQLGRLLINSFKETTTNLLKAMRSAESQGRCEIMLCVERILRGLGVSAVSCHRDIYKAARTGLTDRSMAVRCAAAKCLLELQREAVFLWSTELENVATLCFRALEGSNYDVRVSISKLLGTLLASALEPRQAIAPRPGSKRSSLEEVMELLSSGFLRGGAGFLRASGDMLKGTSSVSRDVRVGITQTCVVFVSILGGVWLETHFSRLLSLLMEWASHTRATQYPADAVSCRCCVSFILRATLGTLLGEKAQIAAAKEICQVISKQKRVVDASLHEGNMETRVSPADVAASQHVLVCALLELGSLVQDLSSTAAPLLQDTGIGMLDTVISVLLHPSASARLASAWCLRCIAVGMPAQVAVLLDRCVERLNALKSCPEAVAGYSAAIAALLGAVQLSPLGISHSKGKMVMTLAEDLLRSAAQNSRISIQRTQGGWLLLSALSTLGPTVMEHHLPRLLLLWKCAFPLSVKDVEMELRRGDSFTWQVTLEGRAGALCAMKSLVVHCKELLTDDVICRFVPLLSCAVALLTQLPSIIKSYGNQIKNAATVFKLRLYEILKLLQPKIYEESFGTVLKQLLNDLTGPEITACAERNLLPSLCYSQDLALLGPGLQDMDQRYIEEQLHGGGSGGGTLEYDSFTIFEKSQEVPTPLPPASALTVAAVQLFGVIFPHLSVQQRAQVLEQFCEFVRQLKGARQQTVQIHVTAAFFCTLKSLASSRSELGPEEVQRPALSLLLGALEGSNPLLRCMAAEGLARLVQVLNDPGFTVSLTLMSFDKLKTARDAITRTGHALALGTVYRYLGGISSPQYLSACVGVLFTLSQDNTSPEVKMWALHALSTVVDLAGPLYHSHLEASFTLVLRLLLSTPHTHMEVQQSLGRCLNALITSVGPDLQGEGAGVCAVRTSCLVGCAVMQDSQDCLVQAQAISCLQQLHMFAPRFVNLSSLVPSLCEILLDYSVLINLCSSYLSLRRAVVACLRQLAQREAVEVSEHAVALVKELPRRDNTQLDVTIKEVGLEGALFSLLDRESDPRLCRDIQETLVHMMSSAAESKLAHWLKLCKDVLSASADSAAAASVETQQEEDGDRYDDSSAFHAKSESSGPFNNLRWSTRVFSMECVCRIIAQCENRDSAHFNMALAQEQRLHESTDFLVLHLADLIRMAFMASTDHSDQLRLAGLQTLLVIIRKFSNVPEPEFPGHVILEQYQANVGAALRPAFHVDAPPNVTAKACQVCSAWIASGVISDLRDLRRVHQLLASSLAKVQVGRDVPSQLYNESTFTMESLAVLKAWAEVYITAVQGSRQRDSPVSHQQQQSEEAGTAGQAGAGLLKLVQTDLATLSRLWLAALQDHALLTLPPHYSSHLPSTGGSFYTAETVEQARPHYYSAWATILHATALWLNSTGFIVVDEGPANLSRPVTPTSMGQSTSLSNVKSPEDINSDRLHLILGISVEFLCSPHSGDQMENIHSCLQALQALLEVPWPRSKVGNDQALSVELLSILHRLIVTRESPSIQLAVLELVQQIICAAQEHVREKRHSAEVDDGAAEKETVPEFGEGRDTGGLVPGRSLVFGALELCLCTLVRKLPQLSPKLTGSPTGRGGQTCSLSATDCRLVTSALAILSELPSICSPEGSVSVLPTVLYLLLGVLKEAVKGSVGTESGQLVSGILQALRTLLTSPMSRAEKSRGAWTQLLQCALHTLLESWSTDKVEPGVDEVTMLTSLTIFLLYASAEVTSVEPLQTRCIQTFRASLDSKDPVVLSRSYQLLLSLFQGPAPVARPFILALGGRLVSQLEEAEKSRPQGPAELQAVQDAIRALEALVFAADETHRPQLVAVLLPILISLLLDENALASAPAASRLLHESALRDLMRIGPQHSAVLKALMASAPHMKARLEAAVKGNQESVNSKAQAPPAISKNTPSIQLKINFL

Target ORF information:

RefSeq Version	XM_009293121.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293121.1

ORF Insert Sequence:

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTGAGATC TTGTTGGATT ATTCTGTGTT GATAAATCTG 
TGCAGCTCAT ACCTGTCTCT GCGGCGAGCG GTTGTGGCCT GTTTAAGACA GCTGGCTCAG 
AGAGAGGCAG TGGAGGTATC GGAACACGCA GTGGCTCTGG TTAAAGAGCT TCCCCGCAGA 
GACAACACAC AGCTGGATGT CACTATTAAG GAGGTGGGCT TGGAGGGAGC TTTGTTCAGT 
CTCCTGGACA GGGAGTCGGA TCCCCGCCTG TGCAGAGACA TTCAGGAAAC ACTGGTCCAT 
ATGATGAGTT CAGCAGCTGA GAGTAAGCTG GCCCACTGGC TGAAGCTCTG CAAAGATGTA 
CTGTCCGCTT CTGCAGACTC CGCTGCAGCC GCCTCTGTAG AAACCCAGCA AGAGGAGGAT 
GGAGATCGAT ATGATGACTC CTCTGCATTC CATGCAAAAT CTGAGTCCAG CGGGCCCTTC 
AATAACCTGC GCTGGTCCAC TCGTGTGTTT TCTATGGAGT GTGTGTGTCG CATAATAGCA 
CAGTGTGAGA ACAGAGACTC AGCTCACTTT AACATGGCAC TTGCTCAAGA GCAGCGTTTA 
CATGAGTCCA CAGATTTCCT GGTTCTTCAC TTGGCGGATC TGATCCGCAT GGCGTTCATG 
GCATCCACAG ACCACAGTGA CCAGCTCCGA CTGGCTGGTT TGCAAACGCT TCTGGTCATT 
ATTCGTAAAT TCTCCAATGT ACCAGAGCCT GAGTTCCCTG GGCATGTCAT TCTGGAGCAG 
TATCAAGCCA ATGTTGGAGC TGCGCTTAGA CCTGCCTTCC ATGTGGACGC TCCTCCAAAC 
GTGACCGCCA AAGCTTGCCA GGTCTGCAGT GCATGGATCG CCAGTGGGGT CATCAGTGAC 
CTGCGGGACC TGAGAAGGGT ACATCAACTT TTGGCATCCT CTCTGGCCAA AGTGCAGGTG 
GGGAGAGATG TTCCCAGTCA GCTCTATAAT GAAAGCACCT TCACCATGGA GTCGCTGGCG 
GTGCTCAAAG CCTGGGCGGA GGTCTACATC ACAGCAGTGC AGGGCTCTAG GCAGAGGGAC 
AGTCCGGTCA GTCATCAGCA GCAGCAGAGT GAGGAGGCTG GGACTGCAGG TCAAGCAGGG 
GCTGGTTTGC TCAAACTAGT TCAGACTGAT CTGGCAACCC TGAGTCGCCT ATGGCTGGCA 
GCCCTTCAGG ATCATGCCCT GCTCACACTG CCCCCACACT ACTCTAGTCA TCTTCCCTCC 
ACAGGCGGCT CATTTTACAC TGCAGAAACG GTGGAACAGG CCCGTCCACA TTACTACAGT 
GCCTGGGCAA CCATTCTCCA TGCCACTGCC CTCTGGCTCA ACAGCACCGG CTTTATCGTG 
GTGGATGAGG GCCCTGCAAA CCTTTCCAGG CCCGTCACGC CCACTTCCAT GGGCCAGTCC 
ACATCACTGA GCAACGTTAA ATCTCCAGAG GACATCAACA GTGATCGCCT ACACCTCATC 
CTTGGGATCA GTGTGGAGTT TTTGTGCTCT CCTCATTCTG GAGACCAAAT GGAAAACATT 
CATTCATGTC TTCAGGCTCT GCAGGCCTTA TTGGAGGTGC CTTGGCCTCG CTCAAAAGTG 
GGCAATGATC AGGCTCTGAG TGTGGAGCTG CTTAGCATCC TGCATAGGCT CATAGTCACT 
CGGGAATCTC CCAGCATTCA GCTGGCTGTG CTGGAACTGG TGCAGCAGAT TATTTGTGCA 
GCTCAGGAAC ACGTCAGAGA AAAACGCCAC AGCGCAGAGG TGGATGACGG TGCGGCAGAG 
AAGGAAACAG TTCCTGAGTT TGGTGAGGGA CGAGACACTG GTGGCCTGGT GCCAGGGAGA 
TCTCTCGTGT TTGGAGCACT GGAGCTGTGT CTCTGCACGC TTGTGAGGAA GCTTCCGCAG 
CTCAGCCCCA AACTGACCGG CAGTCCCACG GGACGGGGAG GACAGACGTG CTCCCTCAGC 
GCCACTGACT GCAGACTCGT CACATCTGCT CTGGCCATCC TCTCTGAACT GCCCTCCATC 
TGTTCACCAG AGGGCAGTGT GTCAGTGCTG CCCACAGTGC TGTATCTGCT GCTCGGGGTT 
TTGAAAGAGG CAGTGAAAGG CTCTGTTGGA ACTGAGAGTG GACAGCTGGT GTCAGGCATC 
CTGCAGGCCC TGCGCACTCT TCTCACTTCT CCCATGAGCA GAGCTGAGAA GAGTCGTGGG 
GCCTGGACTC AACTACTGCA ATGCGCACTT CACACACTCC TGGAGTCCTG GAGTACAGAT 
AAAGTGGAGC CTGGAGTAGA TGAGGTCACC ATGTTGACAT CGCTCACCAT CTTCCTGCTG 
TACGCCAGCG CTGAGGTCAC CTCTGTAGAG CCACTGCAAA CCCGCTGCAT CCAGACATTC 
AGAGCAAGTC TGGACTCCAA AGACCCCGTG GTGCTGAGCA GGAGTTACCA GTTGCTATTA 
TCTTTATTTC AAGGCCCAGC GCCAGTAGCA AGGCCATTTA TTCTGGCTCT AGGTGGTCGT 
CTGGTGTCTC AACTGGAGGA AGCTGAAAAG AGTCGTCCTC AAGGCCCAGC AGAGCTACAG 
GCCGTACAAG ATGCAATCCG GGCCCTGGAA GCACTAGTGT TTGCTGCGGA TGAAACTCAC 
CGTCCTCAGC TGGTGGCTGT GCTGCTGCCT ATCCTCATCT CCCTCCTCTT AGATGAGAAT 
GCTTTGGCAT CGGCTCCGGC GGCTTCTCGC TTGCTGCACG AATCTGCCTT GAGAGACCTC 
ATGCGCATCG GCCCCCAGCA CTCGGCTGTC TTAAAAGCGC TGATGGCCTC CGCTCCGCAC 
ATGAAAGCCA GACTGGAAGC AGCAGTCAAG GGGAACCAGG AGAGCGTGAA CTCTAAAGCA 
CAAGCACCTC CAGCCATCAG CAAAAACACA CCCAGCATTC AACTGAAGAT CAATTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

heatr5a ( XM_009293122.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291397.1 Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X2, mRNA.

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CloneID	ODa40238
Clone ID Related Accession (Same CDS sequence)	XM_009293121.1 , XM_009293122.1
Accession Version	XM_009293122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTGAGATC TTGTTGGATT ATTCTGTGTT GATAAATCTG 
TGCAGCTCAT ACCTGTCTCT GCGGCGAGCG GTTGTGGCCT GTTTAAGACA GCTGGCTCAG 
AGAGAGGCAG TGGAGGTATC GGAACACGCA GTGGCTCTGG TTAAAGAGCT TCCCCGCAGA 
GACAACACAC AGCTGGATGT CACTATTAAG GAGGTGGGCT TGGAGGGAGC TTTGTTCAGT 
CTCCTGGACA GGGAGTCGGA TCCCCGCCTG TGCAGAGACA TTCAGGAAAC ACTGGTCCAT 
ATGATGAGTT CAGCAGCTGA GAGTAAGCTG GCCCACTGGC TGAAGCTCTG CAAAGATGTA 
CTGTCCGCTT CTGCAGACTC CGCTGCAGCC GCCTCTGTAG AAACCCAGCA AGAGGAGGAT 
GGAGATCGAT ATGATGACTC CTCTGCATTC CATGCAAAAT CTGAGTCCAG CGGGCCCTTC 
AATAACCTGC GCTGGTCCAC TCGTGTGTTT TCTATGGAGT GTGTGTGTCG CATAATAGCA 
CAGTGTGAGA ACAGAGACTC AGCTCACTTT AACATGGCAC TTGCTCAAGA GCAGCGTTTA 
CATGAGTCCA CAGATTTCCT GGTTCTTCAC TTGGCGGATC TGATCCGCAT GGCGTTCATG 
GCATCCACAG ACCACAGTGA CCAGCTCCGA CTGGCTGGTT TGCAAACGCT TCTGGTCATT 
ATTCGTAAAT TCTCCAATGT ACCAGAGCCT GAGTTCCCTG GGCATGTCAT TCTGGAGCAG 
TATCAAGCCA ATGTTGGAGC TGCGCTTAGA CCTGCCTTCC ATGTGGACGC TCCTCCAAAC 
GTGACCGCCA AAGCTTGCCA GGTCTGCAGT GCATGGATCG CCAGTGGGGT CATCAGTGAC 
CTGCGGGACC TGAGAAGGGT ACATCAACTT TTGGCATCCT CTCTGGCCAA AGTGCAGGTG 
GGGAGAGATG TTCCCAGTCA GCTCTATAAT GAAAGCACCT TCACCATGGA GTCGCTGGCG 
GTGCTCAAAG CCTGGGCGGA GGTCTACATC ACAGCAGTGC AGGGCTCTAG GCAGAGGGAC 
AGTCCGGTCA GTCATCAGCA GCAGCAGAGT GAGGAGGCTG GGACTGCAGG TCAAGCAGGG 
GCTGGTTTGC TCAAACTAGT TCAGACTGAT CTGGCAACCC TGAGTCGCCT ATGGCTGGCA 
GCCCTTCAGG ATCATGCCCT GCTCACACTG CCCCCACACT ACTCTAGTCA TCTTCCCTCC 
ACAGGCGGCT CATTTTACAC TGCAGAAACG GTGGAACAGG CCCGTCCACA TTACTACAGT 
GCCTGGGCAA CCATTCTCCA TGCCACTGCC CTCTGGCTCA ACAGCACCGG CTTTATCGTG 
GTGGATGAGG GCCCTGCAAA CCTTTCCAGG CCCGTCACGC CCACTTCCAT GGGCCAGTCC 
ACATCACTGA GCAACGTTAA ATCTCCAGAG GACATCAACA GTGATCGCCT ACACCTCATC 
CTTGGGATCA GTGTGGAGTT TTTGTGCTCT CCTCATTCTG GAGACCAAAT GGAAAACATT 
CATTCATGTC TTCAGGCTCT GCAGGCCTTA TTGGAGGTGC CTTGGCCTCG CTCAAAAGTG 
GGCAATGATC AGGCTCTGAG TGTGGAGCTG CTTAGCATCC TGCATAGGCT CATAGTCACT 
CGGGAATCTC CCAGCATTCA GCTGGCTGTG CTGGAACTGG TGCAGCAGAT TATTTGTGCA 
GCTCAGGAAC ACGTCAGAGA AAAACGCCAC AGCGCAGAGG TGGATGACGG TGCGGCAGAG 
AAGGAAACAG TTCCTGAGTT TGGTGAGGGA CGAGACACTG GTGGCCTGGT GCCAGGGAGA 
TCTCTCGTGT TTGGAGCACT GGAGCTGTGT CTCTGCACGC TTGTGAGGAA GCTTCCGCAG 
CTCAGCCCCA AACTGACCGG CAGTCCCACG GGACGGGGAG GACAGACGTG CTCCCTCAGC 
GCCACTGACT GCAGACTCGT CACATCTGCT CTGGCCATCC TCTCTGAACT GCCCTCCATC 
TGTTCACCAG AGGGCAGTGT GTCAGTGCTG CCCACAGTGC TGTATCTGCT GCTCGGGGTT 
TTGAAAGAGG CAGTGAAAGG CTCTGTTGGA ACTGAGAGTG GACAGCTGGT GTCAGGCATC 
CTGCAGGCCC TGCGCACTCT TCTCACTTCT CCCATGAGCA GAGCTGAGAA GAGTCGTGGG 
GCCTGGACTC AACTACTGCA ATGCGCACTT CACACACTCC TGGAGTCCTG GAGTACAGAT 
AAAGTGGAGC CTGGAGTAGA TGAGGTCACC ATGTTGACAT CGCTCACCAT CTTCCTGCTG 
TACGCCAGCG CTGAGGTCAC CTCTGTAGAG CCACTGCAAA CCCGCTGCAT CCAGACATTC 
AGAGCAAGTC TGGACTCCAA AGACCCCGTG GTGCTGAGCA GGAGTTACCA GTTGCTATTA 
TCTTTATTTC AAGGCCCAGC GCCAGTAGCA AGGCCATTTA TTCTGGCTCT AGGTGGTCGT 
CTGGTGTCTC AACTGGAGGA AGCTGAAAAG AGTCGTCCTC AAGGCCCAGC AGAGCTACAG 
GCCGTACAAG ATGCAATCCG GGCCCTGGAA GCACTAGTGT TTGCTGCGGA TGAAACTCAC 
CGTCCTCAGC TGGTGGCTGT GCTGCTGCCT ATCCTCATCT CCCTCCTCTT AGATGAGAAT 
GCTTTGGCAT CGGCTCCGGC GGCTTCTCGC TTGCTGCACG AATCTGCCTT GAGAGACCTC 
ATGCGCATCG GCCCCCAGCA CTCGGCTGTC TTAAAAGCGC TGATGGCCTC CGCTCCGCAC 
ATGAAAGCCA GACTGGAAGC AGCAGTCAAG GGGAACCAGG AGAGCGTGAA CTCTAAAGCA 
CAAGCACCTC CAGCCATCAG CAAAAACACA CCCAGCATTC AACTGAAGAT CAATTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291397.1
CDS	218..6340
Translation	MEQAHSLLLNEEACSQLPEHQRAEFVFEWLRFLKKLLPATDRADVKQKQKRLVEQLTTVLTSSPGPPTRLLLAQCLALVYRVGDSLTSSLTVDRCNDIIRSKDDSPSFLPTRLAAVACLGALYEQLGRLLINSFKETTTNLLKAMRSAESQGRCEIMLCVERILRGLGVSAVSCHRDIYKAARTGLTDRSMAVRCAAAKCLLELQREAVFLWSTELENVATLCFRALEGSNYDVRVSISKLLGTLLASALEPRQAIAPRPGSKRSSLEEVMELLSSGFLRGGAGFLRASGDMLKGTSSVSRDVRVGITQTCVVFVSILGGVWLETHFSRLLSLLMEWASHTRATQYPADAVSCRCCVSFILRATLGTLLGEKAQIAAAKEICQVISKQKRVVDASLHEGNMETRVSPADVAASQHVLVCALLELGSLVQDLSSTAAPLLQDTGIGMLDTVISVLLHPSASARLASAWCLRCIAVGMPAQVAVLLDRCVERLNALKSCPEAVAGYSAAIAALLGAVQLSPLGISHSKGKMVMTLAEDLLRSAAQNSRISIQRTQGGWLLLSALSTLGPTVMEHHLPRLLLLWKCAFPLSVKDVEMELRRGDSFTWQVTLEGRAGALCAMKSLVVHCKELLTDDVICRFVPLLSCAVALLTQLPSIIKSYGNQIKNAATVFKLRLYEILKLLQPKIYEESFGTVLKQLLNDLTGPEITACAERNLLPSLCYSQDLALLGPGLQDMDQRYIEEQLHGGGSGGGTLEYDSFTIFEKSQEVPTPLPPASALTVAAVQLFGVIFPHLSVQQRAQVLEQFCEFVRQLKGARQQTVQIHVTAAFFCTLKSLASSRSELGPEEVQRPALSLLLGALEGSNPLLRCMAAEGLARLVQVLNDPGFTVSLTLMSFDKLKTARDAITRTGHALALGTVYRYLGGISSPQYLSACVGVLFTLSQDNTSPEVKMWALHALSTVVDLAGPLYHSHLEASFTLVLRLLLSTPHTHMEVQQSLGRCLNALITSVGPDLQGEGAGVCAVRTSCLVGCAVMQDSQDCLVQAQAISCLQQLHMFAPRFVNLSSLVPSLCEILLDYSVLINLCSSYLSLRRAVVACLRQLAQREAVEVSEHAVALVKELPRRDNTQLDVTIKEVGLEGALFSLLDRESDPRLCRDIQETLVHMMSSAAESKLAHWLKLCKDVLSASADSAAAASVETQQEEDGDRYDDSSAFHAKSESSGPFNNLRWSTRVFSMECVCRIIAQCENRDSAHFNMALAQEQRLHESTDFLVLHLADLIRMAFMASTDHSDQLRLAGLQTLLVIIRKFSNVPEPEFPGHVILEQYQANVGAALRPAFHVDAPPNVTAKACQVCSAWIASGVISDLRDLRRVHQLLASSLAKVQVGRDVPSQLYNESTFTMESLAVLKAWAEVYITAVQGSRQRDSPVSHQQQQSEEAGTAGQAGAGLLKLVQTDLATLSRLWLAALQDHALLTLPPHYSSHLPSTGGSFYTAETVEQARPHYYSAWATILHATALWLNSTGFIVVDEGPANLSRPVTPTSMGQSTSLSNVKSPEDINSDRLHLILGISVEFLCSPHSGDQMENIHSCLQALQALLEVPWPRSKVGNDQALSVELLSILHRLIVTRESPSIQLAVLELVQQIICAAQEHVREKRHSAEVDDGAAEKETVPEFGEGRDTGGLVPGRSLVFGALELCLCTLVRKLPQLSPKLTGSPTGRGGQTCSLSATDCRLVTSALAILSELPSICSPEGSVSVLPTVLYLLLGVLKEAVKGSVGTESGQLVSGILQALRTLLTSPMSRAEKSRGAWTQLLQCALHTLLESWSTDKVEPGVDEVTMLTSLTIFLLYASAEVTSVEPLQTRCIQTFRASLDSKDPVVLSRSYQLLLSLFQGPAPVARPFILALGGRLVSQLEEAEKSRPQGPAELQAVQDAIRALEALVFAADETHRPQLVAVLLPILISLLLDENALASAPAASRLLHESALRDLMRIGPQHSAVLKALMASAPHMKARLEAAVKGNQESVNSKAQAPPAISKNTPSIQLKINFL

Target ORF information:

RefSeq Version	XM_009293122.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293122.1

ORF Insert Sequence:

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTGAGATC TTGTTGGATT ATTCTGTGTT GATAAATCTG 
TGCAGCTCAT ACCTGTCTCT GCGGCGAGCG GTTGTGGCCT GTTTAAGACA GCTGGCTCAG 
AGAGAGGCAG TGGAGGTATC GGAACACGCA GTGGCTCTGG TTAAAGAGCT TCCCCGCAGA 
GACAACACAC AGCTGGATGT CACTATTAAG GAGGTGGGCT TGGAGGGAGC TTTGTTCAGT 
CTCCTGGACA GGGAGTCGGA TCCCCGCCTG TGCAGAGACA TTCAGGAAAC ACTGGTCCAT 
ATGATGAGTT CAGCAGCTGA GAGTAAGCTG GCCCACTGGC TGAAGCTCTG CAAAGATGTA 
CTGTCCGCTT CTGCAGACTC CGCTGCAGCC GCCTCTGTAG AAACCCAGCA AGAGGAGGAT 
GGAGATCGAT ATGATGACTC CTCTGCATTC CATGCAAAAT CTGAGTCCAG CGGGCCCTTC 
AATAACCTGC GCTGGTCCAC TCGTGTGTTT TCTATGGAGT GTGTGTGTCG CATAATAGCA 
CAGTGTGAGA ACAGAGACTC AGCTCACTTT AACATGGCAC TTGCTCAAGA GCAGCGTTTA 
CATGAGTCCA CAGATTTCCT GGTTCTTCAC TTGGCGGATC TGATCCGCAT GGCGTTCATG 
GCATCCACAG ACCACAGTGA CCAGCTCCGA CTGGCTGGTT TGCAAACGCT TCTGGTCATT 
ATTCGTAAAT TCTCCAATGT ACCAGAGCCT GAGTTCCCTG GGCATGTCAT TCTGGAGCAG 
TATCAAGCCA ATGTTGGAGC TGCGCTTAGA CCTGCCTTCC ATGTGGACGC TCCTCCAAAC 
GTGACCGCCA AAGCTTGCCA GGTCTGCAGT GCATGGATCG CCAGTGGGGT CATCAGTGAC 
CTGCGGGACC TGAGAAGGGT ACATCAACTT TTGGCATCCT CTCTGGCCAA AGTGCAGGTG 
GGGAGAGATG TTCCCAGTCA GCTCTATAAT GAAAGCACCT TCACCATGGA GTCGCTGGCG 
GTGCTCAAAG CCTGGGCGGA GGTCTACATC ACAGCAGTGC AGGGCTCTAG GCAGAGGGAC 
AGTCCGGTCA GTCATCAGCA GCAGCAGAGT GAGGAGGCTG GGACTGCAGG TCAAGCAGGG 
GCTGGTTTGC TCAAACTAGT TCAGACTGAT CTGGCAACCC TGAGTCGCCT ATGGCTGGCA 
GCCCTTCAGG ATCATGCCCT GCTCACACTG CCCCCACACT ACTCTAGTCA TCTTCCCTCC 
ACAGGCGGCT CATTTTACAC TGCAGAAACG GTGGAACAGG CCCGTCCACA TTACTACAGT 
GCCTGGGCAA CCATTCTCCA TGCCACTGCC CTCTGGCTCA ACAGCACCGG CTTTATCGTG 
GTGGATGAGG GCCCTGCAAA CCTTTCCAGG CCCGTCACGC CCACTTCCAT GGGCCAGTCC 
ACATCACTGA GCAACGTTAA ATCTCCAGAG GACATCAACA GTGATCGCCT ACACCTCATC 
CTTGGGATCA GTGTGGAGTT TTTGTGCTCT CCTCATTCTG GAGACCAAAT GGAAAACATT 
CATTCATGTC TTCAGGCTCT GCAGGCCTTA TTGGAGGTGC CTTGGCCTCG CTCAAAAGTG 
GGCAATGATC AGGCTCTGAG TGTGGAGCTG CTTAGCATCC TGCATAGGCT CATAGTCACT 
CGGGAATCTC CCAGCATTCA GCTGGCTGTG CTGGAACTGG TGCAGCAGAT TATTTGTGCA 
GCTCAGGAAC ACGTCAGAGA AAAACGCCAC AGCGCAGAGG TGGATGACGG TGCGGCAGAG 
AAGGAAACAG TTCCTGAGTT TGGTGAGGGA CGAGACACTG GTGGCCTGGT GCCAGGGAGA 
TCTCTCGTGT TTGGAGCACT GGAGCTGTGT CTCTGCACGC TTGTGAGGAA GCTTCCGCAG 
CTCAGCCCCA AACTGACCGG CAGTCCCACG GGACGGGGAG GACAGACGTG CTCCCTCAGC 
GCCACTGACT GCAGACTCGT CACATCTGCT CTGGCCATCC TCTCTGAACT GCCCTCCATC 
TGTTCACCAG AGGGCAGTGT GTCAGTGCTG CCCACAGTGC TGTATCTGCT GCTCGGGGTT 
TTGAAAGAGG CAGTGAAAGG CTCTGTTGGA ACTGAGAGTG GACAGCTGGT GTCAGGCATC 
CTGCAGGCCC TGCGCACTCT TCTCACTTCT CCCATGAGCA GAGCTGAGAA GAGTCGTGGG 
GCCTGGACTC AACTACTGCA ATGCGCACTT CACACACTCC TGGAGTCCTG GAGTACAGAT 
AAAGTGGAGC CTGGAGTAGA TGAGGTCACC ATGTTGACAT CGCTCACCAT CTTCCTGCTG 
TACGCCAGCG CTGAGGTCAC CTCTGTAGAG CCACTGCAAA CCCGCTGCAT CCAGACATTC 
AGAGCAAGTC TGGACTCCAA AGACCCCGTG GTGCTGAGCA GGAGTTACCA GTTGCTATTA 
TCTTTATTTC AAGGCCCAGC GCCAGTAGCA AGGCCATTTA TTCTGGCTCT AGGTGGTCGT 
CTGGTGTCTC AACTGGAGGA AGCTGAAAAG AGTCGTCCTC AAGGCCCAGC AGAGCTACAG 
GCCGTACAAG ATGCAATCCG GGCCCTGGAA GCACTAGTGT TTGCTGCGGA TGAAACTCAC 
CGTCCTCAGC TGGTGGCTGT GCTGCTGCCT ATCCTCATCT CCCTCCTCTT AGATGAGAAT 
GCTTTGGCAT CGGCTCCGGC GGCTTCTCGC TTGCTGCACG AATCTGCCTT GAGAGACCTC 
ATGCGCATCG GCCCCCAGCA CTCGGCTGTC TTAAAAGCGC TGATGGCCTC CGCTCCGCAC 
ATGAAAGCCA GACTGGAAGC AGCAGTCAAG GGGAACCAGG AGAGCGTGAA CTCTAAAGCA 
CAAGCACCTC CAGCCATCAG CAAAAACACA CCCAGCATTC AACTGAAGAT CAATTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

heatr5a ( XM_009293123.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291398.1 Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X3, mRNA.

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CloneID	ODa40239
Clone ID Related Accession (Same CDS sequence)	XM_009293123.1
Accession Version	XM_009293123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	HEAT repeat-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTATAAAT CTGTGCAGCT CATACCTGTC TCTGCGGCGA 
GCGGTTGTGG CCTGTTTAAG ACAGCTGGCT CAGAGAGAGG CAGTGGAGGT ATCGGAACAC 
GCAGTGGCTC TGGTTAAAGA GCTTCCCCGC AGAGACAACA CACAGCTGGA TGTCACTATT 
AAGGAGGTGG GCTTGGAGGG AGCTTTGTTC AGTCTCCTGG ACAGGGAGTC GGATCCCCGC 
CTGTGCAGAG ACATTCAGGA AACACTGGTC CATATGATGA GTTCAGCAGC TGAGAGTAAG 
CTGGCCCACT GGCTGAAGCT CTGCAAAGAT GTACTGTCCG CTTCTGCAGA CTCCGCTGCA 
GCCGCCTCTG TAGAAACCCA GCAAGAGGAG GATGGAGATC GATATGATGA CTCCTCTGCA 
TTCCATGCAA AATCTGAGTC CAGCGGGCCC TTCAATAACC TGCGCTGGTC CACTCGTGTG 
TTTTCTATGG AGTGTGTGTG TCGCATAATA GCACAGTGTG AGAACAGAGA CTCAGCTCAC 
TTTAACATGG CACTTGCTCA AGAGCAGCGT TTACATGAGT CCACAGATTT CCTGGTTCTT 
CACTTGGCGG ATCTGATCCG CATGGCGTTC ATGGCATCCA CAGACCACAG TGACCAGCTC 
CGACTGGCTG GTTTGCAAAC GCTTCTGGTC ATTATTCGTA AATTCTCCAA TGTACCAGAG 
CCTGAGTTCC CTGGGCATGT CATTCTGGAG CAGTATCAAG CCAATGTTGG AGCTGCGCTT 
AGACCTGCCT TCCATGTGGA CGCTCCTCCA AACGTGACCG CCAAAGCTTG CCAGGTCTGC 
AGTGCATGGA TCGCCAGTGG GGTCATCAGT GACCTGCGGG ACCTGAGAAG GGTACATCAA 
CTTTTGGCAT CCTCTCTGGC CAAAGTGCAG GTGGGGAGAG ATGTTCCCAG TCAGCTCTAT 
AATGAAAGCA CCTTCACCAT GGAGTCGCTG GCGGTGCTCA AAGCCTGGGC GGAGGTCTAC 
ATCACAGCAG TGCAGGGCTC TAGGCAGAGG GACAGTCCGG TCAGTCATCA GCAGCAGCAG 
AGTGAGGAGG CTGGGACTGC AGGTCAAGCA GGGGCTGGTT TGCTCAAACT AGTTCAGACT 
GATCTGGCAA CCCTGAGTCG CCTATGGCTG GCAGCCCTTC AGGATCATGC CCTGCTCACA 
CTGCCCCCAC ACTACTCTAG TCATCTTCCC TCCACAGGCG GCTCATTTTA CACTGCAGAA 
ACGGTGGAAC AGGCCCGTCC ACATTACTAC AGTGCCTGGG CAACCATTCT CCATGCCACT 
GCCCTCTGGC TCAACAGCAC CGGCTTTATC GTGGTGGATG AGGGCCCTGC AAACCTTTCC 
AGGCCCGTCA CGCCCACTTC CATGGGCCAG TCCACATCAC TGAGCAACGT TAAATCTCCA 
GAGGACATCA ACAGTGATCG CCTACACCTC ATCCTTGGGA TCAGTGTGGA GTTTTTGTGC 
TCTCCTCATT CTGGAGACCA AATGGAAAAC ATTCATTCAT GTCTTCAGGC TCTGCAGGCC 
TTATTGGAGG TGCCTTGGCC TCGCTCAAAA GTGGGCAATG ATCAGGCTCT GAGTGTGGAG 
CTGCTTAGCA TCCTGCATAG GCTCATAGTC ACTCGGGAAT CTCCCAGCAT TCAGCTGGCT 
GTGCTGGAAC TGGTGCAGCA GATTATTTGT GCAGCTCAGG AACACGTCAG AGAAAAACGC 
CACAGCGCAG AGGTGGATGA CGGTGCGGCA GAGAAGGAAA CAGTTCCTGA GTTTGGTGAG 
GGACGAGACA CTGGTGGCCT GGTGCCAGGG AGATCTCTCG TGTTTGGAGC ACTGGAGCTG 
TGTCTCTGCA CGCTTGTGAG GAAGCTTCCG CAGCTCAGCC CCAAACTGAC CGGCAGTCCC 
ACGGGACGGG GAGGACAGAC GTGCTCCCTC AGCGCCACTG ACTGCAGACT CGTCACATCT 
GCTCTGGCCA TCCTCTCTGA ACTGCCCTCC ATCTGTTCAC CAGAGGGCAG TGTGTCAGTG 
CTGCCCACAG TGCTGTATCT GCTGCTCGGG GTTTTGAAAG AGGCAGTGAA AGGCTCTGTT 
GGAACTGAGA GTGGACAGCT GGTGTCAGGC ATCCTGCAGG CCCTGCGCAC TCTTCTCACT 
TCTCCCATGA GCAGAGCTGA GAAGAGTCGT GGGGCCTGGA CTCAACTACT GCAATGCGCA 
CTTCACACAC TCCTGGAGTC CTGGAGTACA GATAAAGTGG AGCCTGGAGT AGATGAGGTC 
ACCATGTTGA CATCGCTCAC CATCTTCCTG CTGTACGCCA GCGCTGAGGT CACCTCTGTA 
GAGCCACTGC AAACCCGCTG CATCCAGACA TTCAGAGCAA GTCTGGACTC CAAAGACCCC 
GTGGTGCTGA GCAGGAGTTA CCAGTTGCTA TTATCTTTAT TTCAAGGCCC AGCGCCAGTA 
GCAAGGCCAT TTATTCTGGC TCTAGGTGGT CGTCTGGTGT CTCAACTGGA GGAAGCTGAA 
AAGAGTCGTC CTCAAGGCCC AGCAGAGCTA CAGGCCGTAC AAGATGCAAT CCGGGCCCTG 
GAAGCACTAG TGTTTGCTGC GGATGAAACT CACCGTCCTC AGCTGGTGGC TGTGCTGCTG 
CCTATCCTCA TCTCCCTCCT CTTAGATGAG AATGCTTTGG CATCGGCTCC GGCGGCTTCT 
CGCTTGCTGC ACGAATCTGC CTTGAGAGAC CTCATGCGCA TCGGCCCCCA GCACTCGGCT 
GTCTTAAAAG CGCTGATGGC CTCCGCTCCG CACATGAAAG CCAGACTGGA AGCAGCAGTC 
AAGGGGAACC AGGAGAGCGT GAACTCTAAA GCACAAGCAC CTCCAGCCAT CAGCAAAAAC 
ACACCCAGCA TTCAACTGAA GATCAATTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291398.1
CDS	223..6318
Translation	MEQAHSLLLNEEACSQLPEHQRAEFVFEWLRFLKKLLPATDRADVKQKQKRLVEQLTTVLTSSPGPPTRLLLAQCLALVYRVGDSLTSSLTVDRCNDIIRSKDDSPSFLPTRLAAVACLGALYEQLGRLLINSFKETTTNLLKAMRSAESQGRCEIMLCVERILRGLGVSAVSCHRDIYKAARTGLTDRSMAVRCAAAKCLLELQREAVFLWSTELENVATLCFRALEGSNYDVRVSISKLLGTLLASALEPRQAIAPRPGSKRSSLEEVMELLSSGFLRGGAGFLRASGDMLKGTSSVSRDVRVGITQTCVVFVSILGGVWLETHFSRLLSLLMEWASHTRATQYPADAVSCRCCVSFILRATLGTLLGEKAQIAAAKEICQVISKQKRVVDASLHEGNMETRVSPADVAASQHVLVCALLELGSLVQDLSSTAAPLLQDTGIGMLDTVISVLLHPSASARLASAWCLRCIAVGMPAQVAVLLDRCVERLNALKSCPEAVAGYSAAIAALLGAVQLSPLGISHSKGKMVMTLAEDLLRSAAQNSRISIQRTQGGWLLLSALSTLGPTVMEHHLPRLLLLWKCAFPLSVKDVEMELRRGDSFTWQVTLEGRAGALCAMKSLVVHCKELLTDDVICRFVPLLSCAVALLTQLPSIIKSYGNQIKNAATVFKLRLYEILKLLQPKIYEESFGTVLKQLLNDLTGPEITACAERNLLPSLCYSQDLALLGPGLQDMDQRYIEEQLHGGGSGGGTLEYDSFTIFEKSQEVPTPLPPASALTVAAVQLFGVIFPHLSVQQRAQVLEQFCEFVRQLKGARQQTVQIHVTAAFFCTLKSLASSRSELGPEEVQRPALSLLLGALEGSNPLLRCMAAEGLARLVQVLNDPGFTVSLTLMSFDKLKTARDAITRTGHALALGTVYRYLGGISSPQYLSACVGVLFTLSQDNTSPEVKMWALHALSTVVDLAGPLYHSHLEASFTLVLRLLLSTPHTHMEVQQSLGRCLNALITSVGPDLQGEGAGVCAVRTSCLVGCAVMQDSQDCLVQAQAISCLQQLHMFAPRFVNLSSLVPSLCINLCSSYLSLRRAVVACLRQLAQREAVEVSEHAVALVKELPRRDNTQLDVTIKEVGLEGALFSLLDRESDPRLCRDIQETLVHMMSSAAESKLAHWLKLCKDVLSASADSAAAASVETQQEEDGDRYDDSSAFHAKSESSGPFNNLRWSTRVFSMECVCRIIAQCENRDSAHFNMALAQEQRLHESTDFLVLHLADLIRMAFMASTDHSDQLRLAGLQTLLVIIRKFSNVPEPEFPGHVILEQYQANVGAALRPAFHVDAPPNVTAKACQVCSAWIASGVISDLRDLRRVHQLLASSLAKVQVGRDVPSQLYNESTFTMESLAVLKAWAEVYITAVQGSRQRDSPVSHQQQQSEEAGTAGQAGAGLLKLVQTDLATLSRLWLAALQDHALLTLPPHYSSHLPSTGGSFYTAETVEQARPHYYSAWATILHATALWLNSTGFIVVDEGPANLSRPVTPTSMGQSTSLSNVKSPEDINSDRLHLILGISVEFLCSPHSGDQMENIHSCLQALQALLEVPWPRSKVGNDQALSVELLSILHRLIVTRESPSIQLAVLELVQQIICAAQEHVREKRHSAEVDDGAAEKETVPEFGEGRDTGGLVPGRSLVFGALELCLCTLVRKLPQLSPKLTGSPTGRGGQTCSLSATDCRLVTSALAILSELPSICSPEGSVSVLPTVLYLLLGVLKEAVKGSVGTESGQLVSGILQALRTLLTSPMSRAEKSRGAWTQLLQCALHTLLESWSTDKVEPGVDEVTMLTSLTIFLLYASAEVTSVEPLQTRCIQTFRASLDSKDPVVLSRSYQLLLSLFQGPAPVARPFILALGGRLVSQLEEAEKSRPQGPAELQAVQDAIRALEALVFAADETHRPQLVAVLLPILISLLLDENALASAPAASRLLHESALRDLMRIGPQHSAVLKALMASAPHMKARLEAAVKGNQESVNSKAQAPPAISKNTPSIQLKINFL

Target ORF information:

RefSeq Version	XM_009293123.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HEAT repeat containing 5a (heatr5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293123.1

ORF Insert Sequence:

ATGGAGCAGG CTCATAGTTT ACTGCTCAAC GAGGAGGCCT GCAGTCAGCT GCCGGAGCAC 
CAGAGGGCTG AGTTCGTCTT CGAGTGGCTG CGCTTCCTCA AGAAACTTCT CCCTGCCACA 
GACCGGGCTG ATGTAAAACA GAAACAGAAG CGTCTGGTGG AGCAGCTGAC GACAGTACTG 
ACGAGTTCTC CGGGTCCCCC GACGCGTCTG CTGCTGGCAC AGTGCCTAGC GCTAGTGTAT 
CGAGTGGGCG ACTCCCTCAC GTCCAGCCTC ACTGTGGACC GCTGTAATGA TATCATCCGC 
AGCAAGGATG ACTCTCCCAG CTTCCTGCCT ACCCGACTGG CAGCTGTGGC GTGTTTGGGT 
GCGCTGTATG AGCAGCTGGG CCGACTGCTC ATCAACTCAT TTAAAGAGAC CACAACAAAC 
CTTCTGAAAG CTATGAGGAG TGCAGAGTCT CAGGGTCGCT GTGAGATCAT GCTGTGCGTT 
GAGAGGATTC TGAGGGGTTT AGGTGTAAGT GCTGTATCCT GTCACAGAGA CATCTATAAA 
GCTGCTCGCA CTGGTCTCAC TGATCGCTCC ATGGCTGTGC GCTGTGCTGC TGCAAAGTGT 
CTTTTGGAGC TCCAGCGAGA GGCTGTCTTC CTGTGGTCCA CAGAGCTGGA GAATGTGGCC 
ACTCTTTGTT TCCGAGCGCT TGAGGGCTCA AACTACGATG TCCGAGTCAG CATCTCAAAG 
CTGCTTGGCA CTTTATTAGC ATCAGCACTG GAGCCCAGAC AGGCTATAGC TCCGAGGCCG 
GGCTCCAAGC GCAGCTCCCT GGAGGAGGTG ATGGAGCTTC TGAGCTCTGG TTTCCTGCGG 
GGTGGAGCTG GCTTCCTGCG GGCCAGTGGA GACATGCTGA AAGGGACCAG CTCAGTCAGC 
AGAGATGTGC GCGTGGGCAT CACACAGACA TGTGTGGTGT TTGTGTCTAT TCTGGGTGGT 
GTGTGGCTGG AGACGCACTT CTCCAGACTT CTTTCTCTCC TCATGGAGTG GGCATCTCAC 
ACGCGGGCCA CACAATACCC AGCAGATGCT GTGTCTTGCC GCTGCTGTGT CTCCTTCATA 
TTGCGGGCCA CTCTTGGCAC TCTGCTAGGG GAAAAGGCCC AAATTGCCGC TGCCAAGGAG 
ATCTGTCAAG TGATCAGCAA ACAGAAGAGG GTTGTGGATG CATCTCTTCA TGAAGGAAAC 
ATGGAAACGC GTGTGAGCCC TGCGGATGTT GCAGCCAGTC AGCATGTGTT GGTGTGTGCC 
CTGCTGGAGC TGGGCAGTTT GGTACAGGAC CTGAGCTCTA CTGCTGCTCC ATTGTTGCAG 
GACACTGGAA TAGGAATGTT GGACACCGTG ATCTCTGTTC TTCTTCACCC CAGTGCATCT 
GCTCGTCTGG CCTCCGCCTG GTGCCTACGG TGTATTGCCG TAGGGATGCC AGCTCAGGTG 
GCGGTGTTGC TAGACCGCTG TGTAGAGAGG CTCAATGCCC TGAAGTCATG CCCAGAGGCC 
GTGGCAGGAT ACAGTGCTGC CATCGCTGCT CTGCTGGGGG CCGTACAGCT CTCTCCGCTG 
GGAATATCAC ATTCAAAGGG CAAGATGGTG ATGACTTTGG CTGAAGATCT GCTGAGATCT 
GCAGCTCAGA ACAGCAGAAT CTCCATTCAG CGCACTCAAG GAGGCTGGTT GCTGCTCAGT 
GCCCTCTCAA CACTAGGTCC CACTGTAATG GAGCATCACT TGCCTCGATT GCTGTTACTG 
TGGAAATGTG CCTTCCCGCT GTCTGTAAAG GATGTGGAGA TGGAGCTGCG GCGTGGAGAC 
TCTTTCACCT GGCAGGTGAC CCTGGAGGGT CGGGCAGGAG CACTGTGTGC AATGAAGAGT 
CTGGTGGTTC ATTGTAAAGA GCTTCTCACT GATGATGTCA TCTGTCGCTT TGTTCCTCTC 
CTGTCCTGTG CGGTGGCCCT ACTAACTCAG CTGCCGTCTA TTATTAAATC TTATGGAAAT 
CAGATAAAAA ATGCAGCAAC AGTCTTTAAA TTAAGATTGT ATGAGATCCT CAAGTTGCTT 
CAACCTAAAA TCTATGAAGA GAGTTTTGGC ACAGTTCTAA AGCAGCTGCT GAATGATCTG 
ACCGGGCCAG AAATCACAGC ATGTGCTGAG CGGAACCTGC TGCCCTCCCT GTGCTACAGC 
CAAGACCTTG CTCTGCTGGG TCCCGGACTG CAGGATATGG ATCAGCGCTA TATAGAAGAG 
CAGCTGCATG GAGGTGGTTC AGGAGGTGGG ACACTAGAAT ATGACTCATT TACCATCTTT 
GAGAAATCTC AGGAGGTTCC TACTCCGCTC CCTCCAGCCT CAGCTCTAAC AGTGGCTGCT 
GTCCAGCTGT TTGGAGTGAT TTTTCCCCAC CTGAGTGTCC AGCAGAGGGC TCAGGTCCTG 
GAGCAGTTTT GTGAATTTGT GCGACAGCTT AAAGGAGCTC GGCAGCAAAC AGTCCAAATA 
CACGTAACTG CTGCTTTCTT TTGCACTTTG AAGAGTTTGG CATCAAGTCG GAGCGAATTG 
GGGCCAGAGG AGGTCCAAAG ACCAGCATTG TCTCTCCTGT TGGGGGCGTT GGAGGGCTCA 
AACCCTCTAC TGCGCTGCAT GGCTGCAGAG GGTTTAGCAA GGCTGGTGCA AGTTCTTAAT 
GACCCCGGCT TCACAGTTTC CCTCACTCTC ATGAGCTTTG ACAAACTGAA GACAGCAAGG 
GATGCCATCA CACGCACAGG CCATGCCTTG GCCCTCGGAA CGGTTTACCG TTATCTTGGT 
GGGATTAGCT CCCCTCAGTA CCTCAGTGCC TGTGTTGGGG TTCTTTTTAC CCTGTCCCAG 
GATAATACCT CACCAGAAGT AAAGATGTGG GCTCTTCATG CTCTGTCCAC TGTGGTGGAC 
CTAGCAGGTC CTTTGTACCA CAGTCACCTG GAGGCCAGCT TCACTCTGGT GCTGCGTTTG 
CTCCTCTCTA CCCCACACAC ACACATGGAG GTGCAGCAAA GCCTGGGCCG CTGTCTTAAC 
GCCCTCATCA CCTCTGTGGG GCCGGATCTG CAAGGTGAGG GTGCAGGTGT GTGTGCGGTG 
AGGACCTCGT GTTTGGTGGG CTGTGCTGTG ATGCAGGATA GCCAGGACTG TTTAGTCCAG 
GCTCAGGCCA TCTCTTGCCT CCAGCAGCTG CACATGTTTG CGCCACGCTT CGTCAATCTG 
TCCAGCCTTG TGCCAAGTCT CTGTATAAAT CTGTGCAGCT CATACCTGTC TCTGCGGCGA 
GCGGTTGTGG CCTGTTTAAG ACAGCTGGCT CAGAGAGAGG CAGTGGAGGT ATCGGAACAC 
GCAGTGGCTC TGGTTAAAGA GCTTCCCCGC AGAGACAACA CACAGCTGGA TGTCACTATT 
AAGGAGGTGG GCTTGGAGGG AGCTTTGTTC AGTCTCCTGG ACAGGGAGTC GGATCCCCGC 
CTGTGCAGAG ACATTCAGGA AACACTGGTC CATATGATGA GTTCAGCAGC TGAGAGTAAG 
CTGGCCCACT GGCTGAAGCT CTGCAAAGAT GTACTGTCCG CTTCTGCAGA CTCCGCTGCA 
GCCGCCTCTG TAGAAACCCA GCAAGAGGAG GATGGAGATC GATATGATGA CTCCTCTGCA 
TTCCATGCAA AATCTGAGTC CAGCGGGCCC TTCAATAACC TGCGCTGGTC CACTCGTGTG 
TTTTCTATGG AGTGTGTGTG TCGCATAATA GCACAGTGTG AGAACAGAGA CTCAGCTCAC 
TTTAACATGG CACTTGCTCA AGAGCAGCGT TTACATGAGT CCACAGATTT CCTGGTTCTT 
CACTTGGCGG ATCTGATCCG CATGGCGTTC ATGGCATCCA CAGACCACAG TGACCAGCTC 
CGACTGGCTG GTTTGCAAAC GCTTCTGGTC ATTATTCGTA AATTCTCCAA TGTACCAGAG 
CCTGAGTTCC CTGGGCATGT CATTCTGGAG CAGTATCAAG CCAATGTTGG AGCTGCGCTT 
AGACCTGCCT TCCATGTGGA CGCTCCTCCA AACGTGACCG CCAAAGCTTG CCAGGTCTGC 
AGTGCATGGA TCGCCAGTGG GGTCATCAGT GACCTGCGGG ACCTGAGAAG GGTACATCAA 
CTTTTGGCAT CCTCTCTGGC CAAAGTGCAG GTGGGGAGAG ATGTTCCCAG TCAGCTCTAT 
AATGAAAGCA CCTTCACCAT GGAGTCGCTG GCGGTGCTCA AAGCCTGGGC GGAGGTCTAC 
ATCACAGCAG TGCAGGGCTC TAGGCAGAGG GACAGTCCGG TCAGTCATCA GCAGCAGCAG 
AGTGAGGAGG CTGGGACTGC AGGTCAAGCA GGGGCTGGTT TGCTCAAACT AGTTCAGACT 
GATCTGGCAA CCCTGAGTCG CCTATGGCTG GCAGCCCTTC AGGATCATGC CCTGCTCACA 
CTGCCCCCAC ACTACTCTAG TCATCTTCCC TCCACAGGCG GCTCATTTTA CACTGCAGAA 
ACGGTGGAAC AGGCCCGTCC ACATTACTAC AGTGCCTGGG CAACCATTCT CCATGCCACT 
GCCCTCTGGC TCAACAGCAC CGGCTTTATC GTGGTGGATG AGGGCCCTGC AAACCTTTCC 
AGGCCCGTCA CGCCCACTTC CATGGGCCAG TCCACATCAC TGAGCAACGT TAAATCTCCA 
GAGGACATCA ACAGTGATCG CCTACACCTC ATCCTTGGGA TCAGTGTGGA GTTTTTGTGC 
TCTCCTCATT CTGGAGACCA AATGGAAAAC ATTCATTCAT GTCTTCAGGC TCTGCAGGCC 
TTATTGGAGG TGCCTTGGCC TCGCTCAAAA GTGGGCAATG ATCAGGCTCT GAGTGTGGAG 
CTGCTTAGCA TCCTGCATAG GCTCATAGTC ACTCGGGAAT CTCCCAGCAT TCAGCTGGCT 
GTGCTGGAAC TGGTGCAGCA GATTATTTGT GCAGCTCAGG AACACGTCAG AGAAAAACGC 
CACAGCGCAG AGGTGGATGA CGGTGCGGCA GAGAAGGAAA CAGTTCCTGA GTTTGGTGAG 
GGACGAGACA CTGGTGGCCT GGTGCCAGGG AGATCTCTCG TGTTTGGAGC ACTGGAGCTG 
TGTCTCTGCA CGCTTGTGAG GAAGCTTCCG CAGCTCAGCC CCAAACTGAC CGGCAGTCCC 
ACGGGACGGG GAGGACAGAC GTGCTCCCTC AGCGCCACTG ACTGCAGACT CGTCACATCT 
GCTCTGGCCA TCCTCTCTGA ACTGCCCTCC ATCTGTTCAC CAGAGGGCAG TGTGTCAGTG 
CTGCCCACAG TGCTGTATCT GCTGCTCGGG GTTTTGAAAG AGGCAGTGAA AGGCTCTGTT 
GGAACTGAGA GTGGACAGCT GGTGTCAGGC ATCCTGCAGG CCCTGCGCAC TCTTCTCACT 
TCTCCCATGA GCAGAGCTGA GAAGAGTCGT GGGGCCTGGA CTCAACTACT GCAATGCGCA 
CTTCACACAC TCCTGGAGTC CTGGAGTACA GATAAAGTGG AGCCTGGAGT AGATGAGGTC 
ACCATGTTGA CATCGCTCAC CATCTTCCTG CTGTACGCCA GCGCTGAGGT CACCTCTGTA 
GAGCCACTGC AAACCCGCTG CATCCAGACA TTCAGAGCAA GTCTGGACTC CAAAGACCCC 
GTGGTGCTGA GCAGGAGTTA CCAGTTGCTA TTATCTTTAT TTCAAGGCCC AGCGCCAGTA 
GCAAGGCCAT TTATTCTGGC TCTAGGTGGT CGTCTGGTGT CTCAACTGGA GGAAGCTGAA 
AAGAGTCGTC CTCAAGGCCC AGCAGAGCTA CAGGCCGTAC AAGATGCAAT CCGGGCCCTG 
GAAGCACTAG TGTTTGCTGC GGATGAAACT CACCGTCCTC AGCTGGTGGC TGTGCTGCTG 
CCTATCCTCA TCTCCCTCCT CTTAGATGAG AATGCTTTGG CATCGGCTCC GGCGGCTTCT 
CGCTTGCTGC ACGAATCTGC CTTGAGAGAC CTCATGCGCA TCGGCCCCCA GCACTCGGCT 
GTCTTAAAAG CGCTGATGGC CTCCGCTCCG CACATGAAAG CCAGACTGGA AGCAGCAGTC 
AAGGGGAACC AGGAGAGCGT GAACTCTAAA GCACAAGCAC CTCCAGCCAT CAGCAAAAAC 
ACACCCAGCA TTCAACTGAA GATCAATTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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heatr5a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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