PRICKLE1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRICKLE1A
Entrez Gene ID	368249
Full Name	prickle homolog 1a
Synonyms	fk71b09,pk1,prickle1,wu:fk71b09
General protein information	Preferred Names prickle homolog 1a Names prickle homolog 1a pk1a prickle-like 1 a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005174210.4	XP_005174267.1	prickle homolog 1a isoform X1
NM_183342.2	NP_899185.2	prickle homolog 1a
XM_017354059.1	XP_017209548.1	prickle homolog 1a isoform X1
XM_005174210.3	XP_005174267.1	prickle homolog 1a isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PRICKLE1	NP_001096004.1
Mus musculus (house mouse)	Prickle1	NP_001028389.1
Rattus norvegicus (Norway rat)	Prickle1	NP_955428.1
Xenopus tropicalis (tropical clawed frog)	prickle1	NP_001016939.1
Pan troglodytes (chimpanzee)	PRICKLE1	XP_001166831.1
Gallus gallus (chicken)	PRICKLE1	XP_416036.2
Homo sapiens (human)	PRICKLE1	NP_001138355.1
	PRICKLE1	XP_002798581.1
Canis lupus familiaris (dog)	PRICKLE1	XP_005637023.1
Danio rerio (zebrafish)	prickle1a	NP_899185.2

Gene Ontology
Bibliography

Related articles in PubMed

Functional and evolutionary insights from the
Reeves WM, Wu Y, Harder MJ, Veeman MT
Development (Cambridge, England)144(18)3375-3387(2017 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Similar to vangl2, loss of zebrafish prickle1a decreases fibronectin protein levels in gastrula embryos.
Title: Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation.

the wild-type Pk1a partially suppresses the gene knockdown retinal defects but not the mutant forms.
Title: Mechanisms of prickle1a function in zebrafish epilepsy and retinal neurogenesis.

Mechanistically, mir206 targets prickle1a (pk1a) mRNA and as a result regulates c-Jun N-terminal protein kinase 2 (JNK2) phosphorylation.
Title: MicroRNA-206 regulates cell movements during zebrafish gastrulation by targeting prickle1a and regulating c-Jun N-terminal kinase 2 phosphorylation.

homeodomain gene previously shown to be involved in biliary development and in kidney cyst formation
Title: Disruption of planar cell polarity activity leads to developmental biliary defects.

Wnt11 and Prickle1a are expressed in the dorsal forerunner cells and regulate Kupffer's vesicle morphogenesis.
Title: Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ.

The following PRICKLE1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRICKLE1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03820	NM_183342.2 Latest version!	Danio rerio prickle homolog 1a (prickle1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa28982	XM_017354059.1 Latest version!	Danio rerio prickle homolog 1a (prickle1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa28982	XM_005174210.4 Latest version!	Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa28982	XM_005174210.3	Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRICKLE1A ( NM_183342.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_899185.2 Danio rerio prickle homolog 1a (prickle1a), mRNA.

Price & Availability↑

CloneID	ODa03820
Clone ID Related Accession (Same CDS sequence)	NM_183342.2
Accession Version	NM_183342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Danio rerio(zebrafish)
Product	prickle homolog 1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY286492.1. On Dec 20, 2003 this sequence version replaced NM_183342.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY286492.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTGG AGAATCACGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAC GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCAC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGCCTCAGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCTGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGACG CGTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCTGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCTGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CTGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA ACAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCGTTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGTTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCCCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCA GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899185.2
CDS	182..2563
Translation	MELENHGGLHMGSKVNKLTLGFQRSSTSDDDSGCALEEYAWVPPGLRPEQVALYFSCLPEDKVPYVNSPGEKQRIKQLLYQLPPHDNEVRYCQSLSEEEKKELHMFSIQRKKEALGRGTLKILPRTVHHAACEHCGERLSGGEMAVCVSRAAAAGQCWHPACFTCSTCSELLVDLIYFYHDGKVHCGRHHAELLKPRCSSCDEIIFADECTEAEGRHWHMKHFSCSECEVILGGQRYIMKDGRPFCCGCFQSLYAEYCQACAQHIGVDHAQMTYDGLHWHATDACFSCAQCKSSLLGCPFLPRQGRIYCSKACSLGEDVQASDSSDSAFQSARSRESRRSVKMGKSSRSADQCRQSLLFSPASNYKFPGLSGNADDTLSKRLSQLNFADNHFWRNREDQEAPEDHEEWAEHDDYMTQLLLKFGEHGMFQDTRTDLSIWTDSELKNKTCSGGNSSLASKKYKADMYWTQSQDGLGDSAYGSHPGPASSRKLQDLDLEHGASFKHEDNKQWFNDSLECITDQLKEADLNIRDSMDSLALSNITGASVDGDIKDKPLLFSLQKFSDLDRCENTSNMGTLNSSMKSLTSDLDRTQVIEEQQEQGVPSPEVHPKPPRLPVLRRSKSQSKTQQVKFSDDVIDNGRYDELKVRQPPMSERTRRRAFQFDEQDHQRPRHHHRRRRSRMSRSDNALHLAPKEEKPRVCFKDDRRKHGLGHNGAQYGDGLRTERFTRLYEDDWCSTCSSSSSESEEEGFFLGQPIPQPRQPRYQYYASDEAAFSTRTKSKQKRGRKGKNCIIS

Target ORF information:

RefSeq Version	NM_183342.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 1a (prickle1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183342.2

ORF Insert Sequence:

ATGGAGCTGG AGAATCACGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAC GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCAC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGCCTCAGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCTGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGACG CGTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCTGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCTGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CTGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA ACAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCGTTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGTTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCCCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCA GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE1A ( XM_005174210.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174267.1 Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28982
Clone ID Related Accession (Same CDS sequence)	XM_005174210.4 , XM_017354059.1 , XM_005174210.3
Accession Version	XM_005174210.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prickle homolog 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174210.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174267.1
CDS	271..2652
Translation	MELENRGGLHMGSKVNKLTLGFQRSSTSDDDSGCALEEYAWVPPGLRPEQVALYFSCLPEDKVPYVNSPGEKQRIKQLLYQLPPHDNEVRYCQSLSEEEKKELHMFSIQRKKEALGRGTLKILPRTVHHAACEHCGERLGGGEMAVCVSRAAAAGQCWHPACFTCSTCSELLVDLIYFYHDGKVHCGRHHAELLKPRCSSCDEIIFADECTEAEGRHWHMKHFSCSECEVILGGQRYIMKDGRPFCCGCFQSLYAEYCQACAQHIGVDHAQMTYDGLHWHATDACFSCAQCKSSLLGCPFLPRQGRIYCSKACSLGEDVQASDSSDSAFQSARSRESRRSVKMGKSSRSADQCRQSLLFSPASNYKFPGLSGNADDTLSKRLSQLNFADNHFWRNREDQEAPEDHEEWAEHDDYMTQLLLKFGEHGMFQDTRTDLSIWTDSELKNKTGSGGNSSLASKKYKADMYWTQSQDGLGDSAYGSHPGPASSRKLQDLDLEHGASFKHEDNKQWFNDSLECITDQLKEADLNIRDSMDSLALSNITGASVDGDIKDKPLLFSLQKFSDLDRCENTSNMGTLNSSMKSLTSDLDRTQVIEEQQEQGVPSPEVHPKPPRLPVLRRSKSQSKTQQVKFSDDVIDNGRYDELKVRQPPMSERTRRRAFQFDEQDHQRPRHHHRRRRSRMSRSDNALHLAPKEEKPRVCFKDDRRKHGLGHNGAQYGDGLRTERFTRLYEDDWCSTCSSSSSESEEEGFFLGQPIPQPRQPRYQYYASDEAAFGTRTKSKQKRGRKGKNCIIS

Target ORF information:

RefSeq Version	XM_005174210.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174210.4

ORF Insert Sequence:

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE1A ( XM_017354059.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209548.1 Danio rerio prickle homolog 1a (prickle1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28982
Clone ID Related Accession (Same CDS sequence)	XM_005174210.4 , XM_017354059.1 , XM_005174210.3
Accession Version	XM_017354059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prickle homolog 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209548.1
CDS	45..2426
Translation	MELENRGGLHMGSKVNKLTLGFQRSSTSDDDSGCALEEYAWVPPGLRPEQVALYFSCLPEDKVPYVNSPGEKQRIKQLLYQLPPHDNEVRYCQSLSEEEKKELHMFSIQRKKEALGRGTLKILPRTVHHAACEHCGERLGGGEMAVCVSRAAAAGQCWHPACFTCSTCSELLVDLIYFYHDGKVHCGRHHAELLKPRCSSCDEIIFADECTEAEGRHWHMKHFSCSECEVILGGQRYIMKDGRPFCCGCFQSLYAEYCQACAQHIGVDHAQMTYDGLHWHATDACFSCAQCKSSLLGCPFLPRQGRIYCSKACSLGEDVQASDSSDSAFQSARSRESRRSVKMGKSSRSADQCRQSLLFSPASNYKFPGLSGNADDTLSKRLSQLNFADNHFWRNREDQEAPEDHEEWAEHDDYMTQLLLKFGEHGMFQDTRTDLSIWTDSELKNKTGSGGNSSLASKKYKADMYWTQSQDGLGDSAYGSHPGPASSRKLQDLDLEHGASFKHEDNKQWFNDSLECITDQLKEADLNIRDSMDSLALSNITGASVDGDIKDKPLLFSLQKFSDLDRCENTSNMGTLNSSMKSLTSDLDRTQVIEEQQEQGVPSPEVHPKPPRLPVLRRSKSQSKTQQVKFSDDVIDNGRYDELKVRQPPMSERTRRRAFQFDEQDHQRPRHHHRRRRSRMSRSDNALHLAPKEEKPRVCFKDDRRKHGLGHNGAQYGDGLRTERFTRLYEDDWCSTCSSSSSESEEEGFFLGQPIPQPRQPRYQYYASDEAAFGTRTKSKQKRGRKGKNCIIS

Target ORF information:

RefSeq Version	XM_017354059.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prickle homolog 1a (prickle1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354059.1

ORF Insert Sequence:

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE1A ( XM_005174210.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174267.1 Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28982
Clone ID Related Accession (Same CDS sequence)	XM_005174210.4 , XM_017354059.1 , XM_005174210.3
Accession Version	XM_005174210.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prickle homolog 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174267.1
CDS	203..2584
Translation	MELENRGGLHMGSKVNKLTLGFQRSSTSDDDSGCALEEYAWVPPGLRPEQVALYFSCLPEDKVPYVNSPGEKQRIKQLLYQLPPHDNEVRYCQSLSEEEKKELHMFSIQRKKEALGRGTLKILPRTVHHAACEHCGERLGGGEMAVCVSRAAAAGQCWHPACFTCSTCSELLVDLIYFYHDGKVHCGRHHAELLKPRCSSCDEIIFADECTEAEGRHWHMKHFSCSECEVILGGQRYIMKDGRPFCCGCFQSLYAEYCQACAQHIGVDHAQMTYDGLHWHATDACFSCAQCKSSLLGCPFLPRQGRIYCSKACSLGEDVQASDSSDSAFQSARSRESRRSVKMGKSSRSADQCRQSLLFSPASNYKFPGLSGNADDTLSKRLSQLNFADNHFWRNREDQEAPEDHEEWAEHDDYMTQLLLKFGEHGMFQDTRTDLSIWTDSELKNKTGSGGNSSLASKKYKADMYWTQSQDGLGDSAYGSHPGPASSRKLQDLDLEHGASFKHEDNKQWFNDSLECITDQLKEADLNIRDSMDSLALSNITGASVDGDIKDKPLLFSLQKFSDLDRCENTSNMGTLNSSMKSLTSDLDRTQVIEEQQEQGVPSPEVHPKPPRLPVLRRSKSQSKTQQVKFSDDVIDNGRYDELKVRQPPMSERTRRRAFQFDEQDHQRPRHHHRRRRSRMSRSDNALHLAPKEEKPRVCFKDDRRKHGLGHNGAQYGDGLRTERFTRLYEDDWCSTCSSSSSESEEEGFFLGQPIPQPRQPRYQYYASDEAAFGTRTKSKQKRGRKGKNCIIS

Target ORF information:

RefSeq Version	XM_005174210.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prickle homolog 1a (prickle1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174210.3

ORF Insert Sequence:

ATGGAGCTGG AGAATCGCGG TGGGCTCCAC ATGGGGTCAA AGGTCAACAA ACTCACCTTG 
GGTTTCCAGC GCAGCTCCAC GTCAGATGAT GACTCCGGCT GCGCTCTGGA GGAGTACGCC 
TGGGTGCCGC CCGGACTCAG ACCAGAGCAG GTCGCGCTGT ATTTCTCATG TCTCCCGGAG 
GATAAAGTCC CGTATGTGAA CAGCCCCGGA GAGAAACAGC GCATTAAACA GCTCCTGTAC 
CAGCTGCCTC CTCACGATAA TGAAGTGCGG TACTGCCAGT CTCTGAGCGA AGAGGAGAAG 
AAGGAGCTGC ACATGTTCAG CATACAGAGA AAGAAAGAGG CGCTGGGACG AGGAACGCTC 
AAAATACTGC CCCGCACCGT ACATCATGCA GCGTGTGAAC ATTGTGGAGA GCGTCTCGGC 
GGGGGGGAGA TGGCGGTGTG TGTGTCGCGG GCGGCAGCAG CAGGTCAGTG TTGGCATCCG 
GCCTGCTTCA CCTGCTCCAC CTGCTCGGAG CTCCTGGTGG ATCTCATTTA CTTCTATCAT 
GATGGGAAAG TGCACTGCGG CCGACACCAC GCTGAGCTCC TGAAGCCCCG CTGCTCCTCC 
TGTGATGAGA TTATATTTGC GGATGAATGC ACAGAAGCAG AAGGCCGGCA CTGGCACATG 
AAGCACTTCT CCTGCAGTGA GTGTGAGGTT ATTCTGGGTG GCCAGCGCTA CATCATGAAG 
GACGGACGGC CGTTCTGCTG CGGGTGCTTC CAGTCGCTGT ATGCCGAGTA CTGCCAGGCC 
TGCGCACAGC ACATCGGTGT GGATCATGCC CAGATGACGT ATGACGGCCT TCACTGGCAC 
GCCACAGATG CTTGTTTCAG CTGCGCTCAG TGTAAAAGCT CCCTGCTGGG CTGTCCGTTT 
CTACCCAGAC AGGGCAGAAT CTACTGCTCC AAAGCCTGCA GTTTGGGAGA AGATGTGCAG 
GCTTCAGACT CCTCCGATTC TGCCTTCCAG TCCGCCAGGT CCCGCGAGTC CCGCCGCAGT 
GTGAAGATGG GAAAAAGCAG TCGTTCCGCA GACCAGTGCC GACAGTCGCT TTTATTTTCA 
CCCGCAAGCA ACTATAAGTT CCCGGGACTC TCCGGAAACG CTGATGACAC CCTGTCCAAA 
CGGCTATCAC AGCTAAACTT TGCTGATAAT CACTTCTGGA GGAATAGAGA GGATCAGGAG 
GCTCCGGAAG ACCACGAGGA ATGGGCCGAA CATGACGACT ACATGACACA ACTCCTGCTC 
AAATTTGGCG AGCACGGGAT GTTTCAGGAC ACCAGGACTG ATCTATCAAT CTGGACGGAT 
TCTGAGCTCA AGAATAAGAC CGGCAGTGGT GGGAACAGCA GTCTGGCAAG TAAGAAATAC 
AAGGCAGACA TGTACTGGAC TCAGTCCCAG GATGGGCTAG GAGACTCTGC GTACGGGAGC 
CACCCCGGTC CCGCCAGCAG CAGAAAGCTC CAGGATCTGG ACCTGGAGCA TGGAGCGAGC 
TTTAAACATG AAGACAATAA GCAGTGGTTT AATGACTCGC TAGAATGCAT CACTGATCAA 
CTTAAAGAAG CTGATCTGAA CATCAGAGAC TCCATGGACT CTTTAGCGCT CTCCAACATC 
ACAGGTGCTT CAGTTGATGG CGATATTAAA GACAAGCCCT TGCTCTTCTC CCTACAAAAG 
TTCTCTGATC TGGATCGCTG TGAGAACACG AGTAACATGG GAACGCTGAA CTCCTCGATG 
AAGAGCCTGA CGTCTGATCT GGACCGAACT CAAGTGATAG AGGAACAGCA GGAGCAGGGC 
GTACCGAGCC CTGAGGTACA CCCCAAACCA CCGCGCTTAC CTGTGCTGAG AAGATCAAAG 
TCCCAGTCCA AAACACAGCA GGTGAAGTTC TCTGATGATG TCATAGATAA CGGGAGATAC 
GATGAGCTGA AGGTTCGACA GCCGCCAATG AGCGAACGAA CTCGTAGACG AGCATTTCAG 
TTTGACGAAC AAGATCATCA GCGGCCGCGG CATCACCACA GAAGAAGAAG AAGCCGCATG 
TCTCGGTCGG ATAACGCACT TCATTTGGCA CCAAAAGAAG AAAAGCCTCG GGTTTGCTTT 
AAAGATGATC GTCGGAAGCA TGGATTGGGT CATAATGGCG CTCAGTATGG CGACGGTTTG 
CGTACGGAGA GATTTACACG TCTTTATGAA GACGATTGGT GCTCCACGTG CTCTTCCTCA 
TCGTCAGAAT CAGAAGAGGA AGGGTTTTTT TTGGGTCAGC CGATTCCTCA ACCTAGACAA 
CCTCGGTACC AGTATTACGC TTCAGATGAA GCTGCTTTCG GCACAAGGAC AAAGTCCAAA 
CAGAAGAGAG GACGGAAAGG CAAAAACTGT ATTATTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional and evolutionary insights from the
Development (Cambridge, England)144(18)3375-3387(2017 09)
Reeves WM,Wu Y,Harder MJ,Veeman MT

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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PRICKLE1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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