mapk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mapk1
Entrez Gene ID	360144
Full Name	mitogen-activated protein kinase 1
Synonyms	zERK2
General protein information	Preferred Names mitogen-activated protein kinase 1 Names mitogen-activated protein kinase 1 ERK2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_182888.2	NP_878308.2	mitogen-activated protein kinase 1

Pathways from BioSystems

Homologies

	MAPK1	XP_001089600.2
Bos taurus (cattle)	MAPK1	NP_786987.1
Gallus gallus (chicken)	MAPK1	NP_989481.1
Caenorhabditis elegans (roundworm)	mpk-1	NP_001022583.1
Oryza sativa (rice)	Os06g0154500	NP_001056846.1
Homo sapiens (human)	MAPK1	NP_002736.3
Canis lupus familiaris (dog)	MAPK1	NP_001104270.1
Rattus norvegicus (Norway rat)	Mapk1	NP_446294.1
Danio rerio (zebrafish)	mapk1	NP_878308.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009207	XP_319983.4
Xenopus tropicalis (tropical clawed frog)	mapk1	NP_001017127.1
Mus musculus (house mouse)	Mapk1	NP_001033752.1
Drosophila melanogaster (fruit fly)	rl	NP_001015122.1
Saccharomyces cerevisiae (baker's yeast)	KSS1	NP_011554.3
Eremothecium gossypii	AGOS_ACL191C	NP_983213.1
Arabidopsis thaliana (thale cress)	MPK6	NP_181907.1
Pan troglodytes (chimpanzee)	MAPK1	XP_515005.2
Kluyveromyces lactis	KLLA0A02497g	XP_451110.1

Gene Ontology
Bibliography

Related articles in PubMed

Relative importance of phosphatidylinositol-3 kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK3/1) signaling during maturational steroid-induced meiotic G2-M1 transition in zebrafish oocytes.
Das D, Nath P, Pal S, Hajra S, Ghosh P, Maitra S
Zygote (Cambridge, England)1-14(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer.
Title: Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer.

ERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish.
Title: ERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish.

These results demonstrate that induction of Hsp70 in response to heat stress is dependent on ERK activation in Pac2 cells.
Title: Heat stress-induced heat shock protein 70 expression is dependent on ERK activation in zebrafish (Danio rerio) cells.

Data define distinct roles for ERK1 and ERK2 in developmental cell migration processes during zebrafish embryogenesis.
Title: Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.

ERK1 and ERK2 target common and distinct gene sets, confirming diverse roles for these kinases during embryogenesis; for ERK2 genes involved in cell-migration, mesendoderm differentiation and patterning were identified.
Title: ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis.

The following mapk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mapk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00813	NM_182888.2 Latest version!	Danio rerio mitogen-activated protein kinase 1 (mapk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mapk1 ( NM_182888.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_878308.2 Danio rerio mitogen-activated protein kinase 1 (mapk1), mRNA.

Price & Availability↑

CloneID	ODa00813
Clone ID Related Accession (Same CDS sequence)	NM_182888.2
Accession Version	NM_182888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050169.1. On Dec 20, 2003 this sequence version replaced NM_182888.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050169.1, BC065868.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACAG CTGCGGTTTC TGCCCCTGCT GGCGGCGGCC CGAACCCCGG ATCCGGTGCC 
GAGATGGTCC GCGGGCAGGC TTTCGATGTC GGGCCGCGTT ACAGCAACCT CTCCTACATC 
GGAGGAGGCG CTTACGGCAT GGTTTGTTCA GCATATGACC GTGACAATAA AGTACGTGTG 
GCGATCAAGA AGATCAGCCC GTTTGAGCAC CAGACGTACT GTCAGCGCAC ACTGCGTGAG 
ATTAAAATCC TGCTCCGCTT CAAGCATGAA AACATCATCG GTATCAACGA CATCATCCGC 
ACGCCCACCA TCGACCAGAT GAAAGACGTT TACATCGTAC AGGACCTGAT GGAGACAGAC 
TTGTACAAGC TGTTGAAGAC GCAGCACTTG AGCAATGACC ACATCTGTTA CTTCCTGTAT 
CAGATCCTGC GCGGCCTGAA GTACATCCAC TCCGCAAACG TTCTTCACCG AGACCTGAAG 
CCATCAAACC TGTTGCTCAA CACTACCTGT GACCTCAAGA TCTGTGATTT TGGGCTCGCT 
CGGGTGGCTG ATCCAGATCA TGACCACACC GGCTTCCTGA CGGAGTATGT AGCCACACGT 
TGGTACCGCG CACCAGAAAT TATGCTCAAT TCTAAGGGTT ACACTAAATC CATTGATATA 
TGGTCTGTTG GTTGTATTCT GGCTGAGATG CTTTCAAATA GACCCATCTT CCCCGGGAAA 
CACTATCTGG ACCAGCTTAA CCACATCTTA GGTATTTTGG GCTCCCCTTC TCAGGAGGAC 
CTGAACTGCA TCATCAACAT TAAAGCAAGA AATTACCTGC TGTCCCTTCC TCTCCGCAGC 
AAAGTGCCCT GGAACCGCCT GTTTCCCAAC GCAGACCCTA AAGCGCTGGA CCTGTTGGAT 
AAGATGTTGA CCTTTAACCC CCATAAGAGG ATTGAGGTGG AGGAAGCGCT CGCTCACCCG 
TACCTGGAGC AGTACTATGA TCCTACAGAT GAGCCTGTTG CTGAGGCTCC GTTCAAGTTC 
GACATGGAGC TGGATGATTT GCCCAAAGAG ACTCTGAAAG AGCTGATCTT TGAAGAGACC 
GCACGCTTCC AGCCAGGCTA CAGACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878308.2
CDS	237..1346
Translation	MATAAVSAPAGGGPNPGSGAEMVRGQAFDVGPRYSNLSYIGGGAYGMVCSAYDRDNKVRVAIKKISPFEHQTYCQRTLREIKILLRFKHENIIGINDIIRTPTIDQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINIKARNYLLSLPLRSKVPWNRLFPNADPKALDLLDKMLTFNPHKRIEVEEALAHPYLEQYYDPTDEPVAEAPFKFDMELDDLPKETLKELIFEETARFQPGYRP

Target ORF information:

RefSeq Version	NM_182888.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 1 (mapk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182888.2

ORF Insert Sequence:

ATGGCGACAG CTGCGGTTTC TGCCCCTGCT GGCGGCGGCC CGAACCCCGG ATCCGGTGCC 
GAGATGGTCC GCGGGCAGGC TTTCGATGTC GGGCCGCGTT ACAGCAACCT CTCCTACATC 
GGAGGAGGCG CTTACGGCAT GGTTTGTTCA GCATATGACC GTGACAATAA AGTACGTGTG 
GCGATCAAGA AGATCAGCCC GTTTGAGCAC CAGACGTACT GTCAGCGCAC ACTGCGTGAG 
ATTAAAATCC TGCTCCGCTT CAAGCATGAA AACATCATCG GTATCAACGA CATCATCCGC 
ACGCCCACCA TCGACCAGAT GAAAGACGTT TACATCGTAC AGGACCTGAT GGAGACAGAC 
TTGTACAAGC TGTTGAAGAC GCAGCACTTG AGCAATGACC ACATCTGTTA CTTCCTGTAT 
CAGATCCTGC GCGGCCTGAA GTACATCCAC TCCGCAAACG TTCTTCACCG AGACCTGAAG 
CCATCAAACC TGTTGCTCAA CACTACCTGT GACCTCAAGA TCTGTGATTT TGGGCTCGCT 
CGGGTGGCTG ATCCAGATCA TGACCACACC GGCTTCCTGA CGGAGTATGT AGCCACACGT 
TGGTACCGCG CACCAGAAAT TATGCTCAAT TCTAAGGGTT ACACTAAATC CATTGATATA 
TGGTCTGTTG GTTGTATTCT GGCTGAGATG CTTTCAAATA GACCCATCTT CCCCGGGAAA 
CACTATCTGG ACCAGCTTAA CCACATCTTA GGTATTTTGG GCTCCCCTTC TCAGGAGGAC 
CTGAACTGCA TCATCAACAT TAAAGCAAGA AATTACCTGC TGTCCCTTCC TCTCCGCAGC 
AAAGTGCCCT GGAACCGCCT GTTTCCCAAC GCAGACCCTA AAGCGCTGGA CCTGTTGGAT 
AAGATGTTGA CCTTTAACCC CCATAAGAGG ATTGAGGTGG AGGAAGCGCT CGCTCACCCG 
TACCTGGAGC AGTACTATGA TCCTACAGAT GAGCCTGTTG CTGAGGCTCC GTTCAAGTTC 
GACATGGAGC TGGATGATTT GCCCAAAGAG ACTCTGAAAG AGCTGATCTT TGAAGAGACC 
GCACGCTTCC AGCCAGGCTA CAGACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Relative importance of phosphatidylinositol-3 kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK3/1) signaling during maturational steroid-induced meiotic G2-M1 transition in zebrafish oocytes.
Zygote (Cambridge, England)1-14(2017 Dec)
Das D,Nath P,Pal S,Hajra S,Ghosh P,Maitra S

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mapk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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