SESTD1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SESTD1
Entrez Gene ID	360133
Full Name	SEC14 and spectrin domains 1
Synonyms	HAPIP,cb248,dZ106A20.1,duo,sb:cb248,si:dz106a20.1,si:dz106a20.2,si:dz42h5.2,sol
General protein information	Preferred Names SEC14 and spectrin domains 1 Names SEC14 domain and spectrin repeat-containing protein 1 huntingtin-associated protein Solo solo
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167875.4	XP_005167932.1	SEC14 domain and spectrin repeat-containing protein 1 isoform X1
NM_182881.2	NP_878301.2	SEC14 domain and spectrin repeat-containing protein 1
XM_005167875.3	XP_005167932.1	SEC14 domain and spectrin repeat-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SESTD1	NP_001230034.1
Drosophila melanogaster (fruit fly)	CG15822	NP_647693.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007560	XP_308320.4
Bos taurus (cattle)	SESTD1	NP_001178179.1
Homo sapiens (human)	SESTD1	NP_835224.3
Canis lupus familiaris (dog)	SESTD1	XP_535985.1
Mus musculus (house mouse)	Sestd1	NP_780674.1
Gallus gallus (chicken)	SESTD1	XP_004942752.1
Caenorhabditis elegans (roundworm)	CELE_F55D12.2	NP_001250623.1
	SESTD1	XP_001099516.1
Rattus norvegicus (Norway rat)	Sestd1	NP_001127986.2
Danio rerio (zebrafish)	sestd1	NP_878301.2
Xenopus tropicalis (tropical clawed frog)	sestd1	NP_001011359.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

CERKL knockdown causes retinal degeneration in zebrafish.
Riera M, Burguera D, Garcia-Fern?ndez J, Gonz?lez-Duarte R
PloS one8(5)e64048(2013)

A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos.
Jin SW, Herzog W, Santoro MM, Mitchell TS, Frantsve J, Jungblut B, Beis D, Scott IC, D'Amico LA, Ober EA, Verkade H, Field HA, Chi NC, Wehman AM, Baier H, Stainier DY
Developmental biology307(1)29-42(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SESTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SESTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15464	NM_182881.2 Latest version!	Danio rerio SEC14 and spectrin domains 1 (sestd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa15464	XM_005167875.4 Latest version!	Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa15464	XM_005167875.3	Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SESTD1 ( NM_182881.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_878301.2 Danio rerio SEC14 and spectrin domains 1 (sestd1), mRNA.

Price & Availability↑

CloneID	ODa15464
Clone ID Related Accession (Same CDS sequence)	XM_005167875.4 , NM_182881.2 , XM_005167875.3
Accession Version	NM_182881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	SEC14 domain and spectrin repeat-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134893.1. On Apr 1, 2007 this sequence version replaced NM_182881.1. ##Evidence-Data-START## Transcript exon combination :: BC134893.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878301.2
CDS	159..2303
Translation	MEASCILPVLKKKLAFLSGGKDRRSGLILTIPLCTEQTSMEELSTTLDYLLGIPSEKCKARGFTVIVDGRKSQWNIVKTVVLMLQNVIPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTDEFGGSLLYDHLDWVNKRLVFEKFTKESTSLLDELIVINENEKGSQAEKDRSSESNILPSFDPETVLQTGHELLSELQQRRFNGSEGGGGGGTAWSPMDDELLAQPQVMKLLDSLREQYTKYQEVCRQRSKRSQLEEIQTKVMQVVNWLEGPGTDQLRTQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQIAALLSAGDEEDLMELKGLQQQLSDVCYRQASQLEFRQNVLQSAYEFHMTAQDLSQQLDGLLGMLCADVAPADGAAIQQTLKHLEEKLKSVESALQTLREKGQALLDQMSNQTSFSYGKEVGVENKENIDHIHSVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDASQAVEWLGELLDALLKTHIRLGDDSQETKVLLEKHKKFVDVAQSTYDYGRQLLQATVVLCQSLRCTTRSSGDTLPRLNRVWKQFTVTSDERQHRLEMASAFHTAAEKIVRESPELAELLVDVEAYEEVETLGKGLLDRLTVPVIFPDGSEQFFGSPGDMASSAESIRERMKLVEEKRFLQEEAEQRLEEEEEEEEAALEVEPRES

Target ORF information:

RefSeq Version	NM_182881.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC14 and spectrin domains 1 (sestd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182881.2

ORF Insert Sequence:

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESTD1 ( XM_005167875.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167932.1 Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15464
Clone ID Related Accession (Same CDS sequence)	XM_005167875.4 , NM_182881.2 , XM_005167875.3
Accession Version	XM_005167875.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SEC14 domain and spectrin repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167875.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167932.1
CDS	233..2377
Translation	MEASCILPVLKKKLAFLSGGKDRRSGLILTIPLCTEQTSMEELSTTLDYLLGIPSEKCKARGFTVIVDGRKSQWNIVKTVVLMLQNVIPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTDEFGGSLLYDHLDWVNKRLVFEKFTKESTSLLDELIVINENEKGSQAEKDRSSESNILPSFDPETVLQTGHELLSELQQRRFNGSEGGGGGGTAWSPMDDELLAQPQVMKLLDSLREQYTKYQEVCRQRSKRSQLEEIQTKVMQVVNWLEGPGTDQLRTQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQIAALLSAGDEEDLMELKGLQQQLSDVCYRQASQLEFRQNVLQSAYEFHMTAQDLSQQLDGLLGMLCADVAPADGAAIQQTLKHLEEKLKSVESALQTLREKGQALLDQMSNQTSFSYGKEVGVENKENIDHIHSVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDASQAVEWLGELLDALLKTHIRLGDDSQETKVLLEKHKKFVDVAQSTYDYGRQLLQATVVLCQSLRCTTRSSGDTLPRLNRVWKQFTVTSDERQHRLEMASAFHTAAEKIVRESPELAELLVDVEAYEEVETLGKGLLDRLTVPVIFPDGSEQFFGSPGDMASSAESIRERMKLVEEKRFLQEEAEQRLEEEEEEEEAALEVEPRES

Target ORF information:

RefSeq Version	XM_005167875.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167875.4

ORF Insert Sequence:

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESTD1 ( XM_005167875.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167932.1 Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.

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CloneID	ODa15464
Clone ID Related Accession (Same CDS sequence)	XM_005167875.4 , NM_182881.2 , XM_005167875.3
Accession Version	XM_005167875.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SEC14 domain and spectrin repeat-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167875.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167932.1
CDS	211..2355
Translation	MEASCILPVLKKKLAFLSGGKDRRSGLILTIPLCTEQTSMEELSTTLDYLLGIPSEKCKARGFTVIVDGRKSQWNIVKTVVLMLQNVIPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTDEFGGSLLYDHLDWVNKRLVFEKFTKESTSLLDELIVINENEKGSQAEKDRSSESNILPSFDPETVLQTGHELLSELQQRRFNGSEGGGGGGTAWSPMDDELLAQPQVMKLLDSLREQYTKYQEVCRQRSKRSQLEEIQTKVMQVVNWLEGPGTDQLRTQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQIAALLSAGDEEDLMELKGLQQQLSDVCYRQASQLEFRQNVLQSAYEFHMTAQDLSQQLDGLLGMLCADVAPADGAAIQQTLKHLEEKLKSVESALQTLREKGQALLDQMSNQTSFSYGKEVGVENKENIDHIHSVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDASQAVEWLGELLDALLKTHIRLGDDSQETKVLLEKHKKFVDVAQSTYDYGRQLLQATVVLCQSLRCTTRSSGDTLPRLNRVWKQFTVTSDERQHRLEMASAFHTAAEKIVRESPELAELLVDVEAYEEVETLGKGLLDRLTVPVIFPDGSEQFFGSPGDMASSAESIRERMKLVEEKRFLQEEAEQRLEEEEEEEEAALEVEPRES

Target ORF information:

RefSeq Version	XM_005167875.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC14 and spectrin domains 1 (sestd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167875.3

ORF Insert Sequence:

ATGGAAGCTT CCTGTATATT GCCGGTGCTG AAGAAGAAGC TGGCCTTTCT GTCAGGAGGC 
AAGGACAGGC GCAGTGGTTT AATCCTGACC ATCCCACTAT GCACCGAACA GACCAGCATG 
GAGGAGCTCA GCACCACTCT GGACTACCTT CTTGGTATTC CCAGCGAGAA ATGCAAAGCT 
CGGGGTTTCA CAGTCATCGT CGATGGGAGG AAGTCTCAAT GGAATATAGT GAAGACTGTT 
GTTTTGATGT TACAGAACGT GATCCCAGCT GAAGTTTCTC TTGTGTGTGT GGTGAAACCT 
GATGAATTCT GGGACAAAAA AGTGACCCAT TTCTGCTTCT GGAAGGAGAA GGACAGGCTT 
GGCTTTGAGG TGATTCTGGT GTCTGCCAAC AAACTCACAC GCTACATCGA GCCATGCCAG 
CTTACAGATG AGTTTGGTGG GAGTCTGCTT TATGATCATT TGGACTGGGT GAACAAACGA 
TTGGTTTTTG AAAAATTCAC AAAAGAATCC ACGTCTCTTT TGGATGAGCT TATCGTCATC 
AATGAAAACG AGAAGGGAAG CCAGGCAGAG AAGGACAGGT CTTCGGAGAG CAATATTCTG 
CCTTCATTTG ATCCTGAAAC GGTTCTTCAA ACTGGACACG AACTGCTCTC AGAGCTCCAG 
CAGAGGCGTT TCAATGGTTC TGAGGGTGGA GGAGGTGGAG GAACAGCCTG GTCACCCATG 
GACGATGAGC TGCTCGCTCA GCCTCAGGTG ATGAAGCTCC TGGACTCTCT GAGGGAGCAG 
TACACAAAGT ATCAGGAAGT CTGTCGGCAG CGCAGCAAAC GCTCCCAGCT AGAGGAAATC 
CAGACCAAGG TCATGCAGGT TGTGAATTGG CTGGAGGGTC CTGGTACAGA CCAGCTCCGC 
ACACAGTGGG GGATTGGAGA CTCCATTCGC GCCTCTCAGG CCCTGCAGCA GAAACATGAA 
GAGATCGAGA GTCAACACAG TGAGTGGTTT GCTGTTTACG TGGAGTTGAA CCAGCAGATT 
GCGGCACTGC TGAGTGCAGG AGATGAAGAG GACCTGATGG AGCTCAAGGG TCTGCAGCAG 
CAGCTCAGTG ACGTCTGCTA CCGACAGGCC AGCCAGTTAG AGTTCCGACA GAACGTCCTG 
CAGTCTGCAT ATGAGTTCCA CATGACCGCA CAAGATCTGT CTCAGCAGTT AGATGGGCTG 
TTGGGAATGC TCTGTGCAGA CGTCGCCCCT GCAGATGGAG CTGCAATCCA GCAAACTCTC 
AAACATCTGG AGGAGAAGCT CAAGAGCGTA GAGAGTGCCT TGCAGACCTT GAGAGAAAAA 
GGCCAAGCGC TGCTTGACCA GATGTCCAAC CAGACATCTT TTTCCTACGG CAAGGAAGTA 
GGGGTTGAGA ACAAGGAGAA TATCGACCAC ATTCACAGCG TCATGGAGGA CATGCAGCTC 
CGCAAACAGA GGTGTGAGGA TATGGTTGAT GTCAGGAGAC TGAAGATGCT GCAGATGGTT 
CAGCTCTTCA AGTGTGAGGA AGATGCTTCA CAGGCAGTAG AGTGGTTGGG AGAGTTGCTC 
GATGCTTTGT TGAAGACCCA CATCCGACTG GGAGATGATT CACAAGAAAC CAAAGTGCTG 
CTGGAGAAAC ACAAGAAGTT TGTGGATGTT GCACAGAGCA CGTATGATTA CGGTCGTCAG 
CTGCTGCAGG CCACAGTGGT GCTGTGCCAG TCTCTGCGCT GTACCACCCG CTCCTCAGGA 
GACACGCTGC CCAGACTCAA CCGCGTGTGG AAACAGTTCA CCGTCACGTC TGATGAGCGC 
CAGCACCGGC TGGAGATGGC CAGCGCCTTT CACACGGCTG CTGAGAAGAT TGTGAGAGAG 
AGCCCAGAGT TGGCAGAGCT CCTGGTAGAT GTGGAGGCTT ATGAAGAGGT GGAGACGCTG 
GGGAAAGGGC TACTGGACAG GCTCACTGTG CCAGTCATTT TCCCTGATGG GAGCGAACAG 
TTTTTCGGTA GTCCGGGTGA CATGGCCTCC TCAGCTGAAA GTATTCGTGA GCGAATGAAG 
CTGGTGGAGG AGAAGAGGTT CCTGCAGGAG GAAGCAGAGC AGAGGCTGGA GGAGGAGGAG 
GAGGAAGAGG AGGCGGCTCT GGAGGTGGAA CCCAGGGAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

CERKL knockdown causes retinal degeneration in zebrafish.
PloS one8(5)e64048(2013)
Riera M,Burguera D,Garcia-Fern?ndez J,Gonz?lez-Duarte R

A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos.
Developmental biology307(1)29-42(2007 Jul)
Jin SW,Herzog W,Santoro MM,Mitchell TS,Frantsve J,Jungblut B,Beis D,Scott IC,D'Amico LA,Ober EA,Verkade H,Field HA,Chi NC,Wehman AM,Baier H,Stainier DY

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SESTD1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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