tfpia cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tfpia
Entrez Gene ID	359833
Full Name	tissue factor pathway inhibitor a
Synonyms	TFPI,si:dz44f8.1,tfpib,wu:fb92g01,zgc:152844,zgc:158592
General protein information	Preferred Names tissue factor pathway inhibitor a Names tissue factor pathway inhibitor coagulation inhibitor lipoprotein-associated tissue factor pathway inhibitor b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_182872.2	NP_878292.2	tissue factor pathway inhibitor isoform 2 precursor
NM_001309803.1	NP_001296732.1	tissue factor pathway inhibitor isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TFPI	NP_006278.1
Pan troglodytes (chimpanzee)	TFPI	XP_003949980.1
Bos taurus (cattle)	TFPI	NP_001231133.1
Mus musculus (house mouse)	Tfpi	NP_035706.1
	TFPI	XP_001104806.1
Rattus norvegicus (Norway rat)	Tfpi	NP_058896.1
Gallus gallus (chicken)	TFPI	XP_004942460.1
Danio rerio (zebrafish)	tfpia	NP_878292.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a new
Wang L, Wang X, Wang L, Yousaf M, Li J, Zuo M, Yang Z, Gou D, Bao B, Li L, Xiang N, Jia H, Xu C, Chen Q, Wang QK
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(1)183-194(2018 Jan)

Bone morphogenetic protein signaling governs biliary-driven liver regeneration in zebrafish through tbx2b and id2a.
Choi TY, Khaliq M, Tsurusaki S, Ninov N, Stainier DYR, Tanaka M, Shin D
Hepatology (Baltimore, Md.)66(5)1616-1630(2017 11)

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Calcitonin receptor family evolution and fishing for function using in silico promoter analysis.
Martins R, Vieira FA, Power DM
General and comparative endocrinology20961-73(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tfpia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tfpia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa15463	NM_001309803.1 Latest version!	Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa40153	NM_182872.2 Latest version!	Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tfpia ( NM_001309803.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296732.1 Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa15463
Clone ID Related Accession (Same CDS sequence)	NM_001309803.1
Accession Version	NM_001309803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tissue factor pathway inhibitor isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT943665.10 and BX470245.8. On Jun 12, 2015 this sequence version replaced XM_005167874.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01001691.1, BC124151.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505377, SAMEA3505382 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCAT ACCTGAACTC TTCATGCAAA CCGCTGCTTC TGCTGTCTCT TTTTGGAGTT 
TGTTTCGCGA GATTTGAAAG ATCAGATGGA GTGAGGTCTG AACTCCGCAT CTTCCATCAA 
TCGTGCGCCC TGAAGAAAGA TGAAGGGCCG TGCAAGGCAA TGAAGGACAG GTTTTACTTT 
GACATCGACA CCGGCCGTTG TGAGCCCTTT GAATATGGAG GCTGTCAAGG CAATGCAAAC 
AACTTTGAGA CCCTGCAAGA CTGCGAGGAA ATGTGTCTTG TGACAGAGAA TAAAAGTCCA 
TGTCATCTGG AGGATGAGCC GGGGCCATGT AGAGGACTGG TGCCTCGCTA TTTTTTTGAT 
CAGAAGAGCC AGGAATGCAA ACAGTTCTTT TATGGTGGAT GCTTTGGGAA CGCCAACAAC 
TTCAAGACCA TAAAAGCATG CCAACAGAGA TGTCAGCTTA CCGCTGTATT AAAGTCAGAG 
GAAGAGGAAG CCAAACCTGT GATAGAATCT CCTGCTATAC ATGATGATGC TCATTTGAAC 
TCATCCCATT CAGCGCTGAG GAGAGTCTCC CAACCAGCTA AACAGGAAGC AGAGTTCAGT 
CCTCCAGAGT TGTGCATGAG TGCAGTTGAC CGGGGGGATT GTGACGGCTC TGAGAGGAGA 
TACGTGTTCA ATCCTAGGAT TGGCAGATGT CAAGTCTTTC GCTACTCTGG ATGTGGAGGA 
AACAAAAACA ACTTCATCCA CAAAAGACAC TGCATGAAAA TGTGCATGAA AGATCAACAC 
CGGAGAAAGC AAATACGGAT AAAGACGAGG AACTCTAATA TCTTATTCCG ATCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296732.1
CDS	137..976
Translation	MAPYLNSSCKPLLLLSLFGVCFARFERSDGVRSELRIFHQSCALKKDEGPCKAMKDRFYFDIDTGRCEPFEYGGCQGNANNFETLQDCEEMCLVTENKSPCHLEDEPGPCRGLVPRYFFDQKSQECKQFFYGGCFGNANNFKTIKACQQRCQLTAVLKSEEEEAKPVIESPAIHDDAHLNSSHSALRRVSQPAKQEAEFSPPELCMSAVDRGDCDGSERRYVFNPRIGRCQVFRYSGCGGNKNNFIHKRHCMKMCMKDQHRRKQIRIKTRNSNILFRSV

Target ORF information:

RefSeq Version	NM_001309803.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309803.1

ORF Insert Sequence:

ATGGCTCCAT ACCTGAACTC TTCATGCAAA CCGCTGCTTC TGCTGTCTCT TTTTGGAGTT 
TGTTTCGCGA GATTTGAAAG ATCAGATGGA GTGAGGTCTG AACTCCGCAT CTTCCATCAA 
TCGTGCGCCC TGAAGAAAGA TGAAGGGCCG TGCAAGGCAA TGAAGGACAG GTTTTACTTT 
GACATCGACA CCGGCCGTTG TGAGCCCTTT GAATATGGAG GCTGTCAAGG CAATGCAAAC 
AACTTTGAGA CCCTGCAAGA CTGCGAGGAA ATGTGTCTTG TGACAGAGAA TAAAAGTCCA 
TGTCATCTGG AGGATGAGCC GGGGCCATGT AGAGGACTGG TGCCTCGCTA TTTTTTTGAT 
CAGAAGAGCC AGGAATGCAA ACAGTTCTTT TATGGTGGAT GCTTTGGGAA CGCCAACAAC 
TTCAAGACCA TAAAAGCATG CCAACAGAGA TGTCAGCTTA CCGCTGTATT AAAGTCAGAG 
GAAGAGGAAG CCAAACCTGT GATAGAATCT CCTGCTATAC ATGATGATGC TCATTTGAAC 
TCATCCCATT CAGCGCTGAG GAGAGTCTCC CAACCAGCTA AACAGGAAGC AGAGTTCAGT 
CCTCCAGAGT TGTGCATGAG TGCAGTTGAC CGGGGGGATT GTGACGGCTC TGAGAGGAGA 
TACGTGTTCA ATCCTAGGAT TGGCAGATGT CAAGTCTTTC GCTACTCTGG ATGTGGAGGA 
AACAAAAACA ACTTCATCCA CAAAAGACAC TGCATGAAAA TGTGCATGAA AGATCAACAC 
CGGAGAAAGC AAATACGGAT AAAGACGAGG AACTCTAATA TCTTATTCCG ATCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tfpia ( NM_182872.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_878292.2 Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa40153
Clone ID Related Accession (Same CDS sequence)	NM_182872.2
Accession Version	NM_182872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tissue factor pathway inhibitor isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT943665.10 and BX470245.8. On Jun 12, 2015 this sequence version replaced NM_182872.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC133127.1, AF515274.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA3505377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCAT ACCTGAACTC TTCATGCAAA CCGCTGCTTC TGCTGTCTCT TTTTGGAGTT 
TGTTTCGCGA GATTTGAAAG ATATGGAGTG AGGTCTGAAC TCCGCATCTT CCATCAATCG 
TGCGCCCTGA AGAAAGATGA AGGGCCGTGC AAGGCAATGA AGGACAGGTT TTACTTTGAC 
ATCGACACCG GCCGTTGTGA GCCCTTTGAA TATGGAGGCT GTCAAGGCAA TGCAAACAAC 
TTTGAGACCC TGCAAGACTG CGAGGAAATG TGTCTTGTGA CAGAGAATAA AAGTCCATGT 
CATCTGGAGG ATGAGCCGGG GCCATGTAGA GGACTGGTGC CTCGCTATTT TTTTGATCAG 
AAGAGCCAGG AATGCAAACA GTTCTTTTAT GGTGGATGCT TTGGGAACGC CAACAACTTC 
AAGACCATAA AAGCATGCCA ACAGAGATGT CAGCTTACCG CTGTATTAAA GTCAGAGGAA 
GAGGAAGCCA AACCTGTGAT AGAATCTCCT GCTATACATG ATGATGCTCA TTTGAACTCA 
TCCCATTCAG CGCTGAGGAG AGTCTCCCAA CCAGCTAAAC AGGAAGCAGA GTTCAGTCCT 
CCAGAGTTGT GCATGAGTGC AGTTGACCGG GGGGATTGTG ACGGCTCTGA GAGGAGATAC 
GTGTTCAATC CTAGGATTGG CAGATGTCAA GTCTTTCGCT ACTCTGGATG TGGAGGAAAC 
AAAAACAACT TCATCCACAA AAGACACTGC ATGAAAATGT GCATGAAAGA TCAACACCGG 
AGAAAGCAAA TACGGATAAA GACGAGGAAC TCTAATATCT TATTCCGATC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878292.2
CDS	137..973
Translation	MAPYLNSSCKPLLLLSLFGVCFARFERYGVRSELRIFHQSCALKKDEGPCKAMKDRFYFDIDTGRCEPFEYGGCQGNANNFETLQDCEEMCLVTENKSPCHLEDEPGPCRGLVPRYFFDQKSQECKQFFYGGCFGNANNFKTIKACQQRCQLTAVLKSEEEEAKPVIESPAIHDDAHLNSSHSALRRVSQPAKQEAEFSPPELCMSAVDRGDCDGSERRYVFNPRIGRCQVFRYSGCGGNKNNFIHKRHCMKMCMKDQHRRKQIRIKTRNSNILFRSV

Target ORF information:

RefSeq Version	NM_182872.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tissue factor pathway inhibitor a (tfpia), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182872.2

ORF Insert Sequence:

ATGGCTCCAT ACCTGAACTC TTCATGCAAA CCGCTGCTTC TGCTGTCTCT TTTTGGAGTT 
TGTTTCGCGA GATTTGAAAG ATATGGAGTG AGGTCTGAAC TCCGCATCTT CCATCAATCG 
TGCGCCCTGA AGAAAGATGA AGGGCCGTGC AAGGCAATGA AGGACAGGTT TTACTTTGAC 
ATCGACACCG GCCGTTGTGA GCCCTTTGAA TATGGAGGCT GTCAAGGCAA TGCAAACAAC 
TTTGAGACCC TGCAAGACTG CGAGGAAATG TGTCTTGTGA CAGAGAATAA AAGTCCATGT 
CATCTGGAGG ATGAGCCGGG GCCATGTAGA GGACTGGTGC CTCGCTATTT TTTTGATCAG 
AAGAGCCAGG AATGCAAACA GTTCTTTTAT GGTGGATGCT TTGGGAACGC CAACAACTTC 
AAGACCATAA AAGCATGCCA ACAGAGATGT CAGCTTACCG CTGTATTAAA GTCAGAGGAA 
GAGGAAGCCA AACCTGTGAT AGAATCTCCT GCTATACATG ATGATGCTCA TTTGAACTCA 
TCCCATTCAG CGCTGAGGAG AGTCTCCCAA CCAGCTAAAC AGGAAGCAGA GTTCAGTCCT 
CCAGAGTTGT GCATGAGTGC AGTTGACCGG GGGGATTGTG ACGGCTCTGA GAGGAGATAC 
GTGTTCAATC CTAGGATTGG CAGATGTCAA GTCTTTCGCT ACTCTGGATG TGGAGGAAAC 
AAAAACAACT TCATCCACAA AAGACACTGC ATGAAAATGT GCATGAAAGA TCAACACCGG 
AGAAAGCAAA TACGGATAAA GACGAGGAAC TCTAATATCT TATTCCGATC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a new
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(1)183-194(2018 Jan)
Wang L,Wang X,Wang L,Yousaf M,Li J,Zuo M,Yang Z,Gou D,Bao B,Li L,Xiang N,Jia H,Xu C,Chen Q,Wang QK

Bone morphogenetic protein signaling governs biliary-driven liver regeneration in zebrafish through tbx2b and id2a.
Hepatology (Baltimore, Md.)66(5)1616-1630(2017 11)
Choi TY,Khaliq M,Tsurusaki S,Ninov N,Stainier DYR,Tanaka M,Shin D

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Calcitonin receptor family evolution and fishing for function using in silico promoter analysis.
General and comparative endocrinology20961-73(2014 Dec)
Martins R,Vieira FA,Power DM