f9a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	f9a
Entrez Gene ID	359826
Full Name	coagulation factor IXa
Synonyms	f9,zgc:109775
General protein information	Preferred Names coagulation factor IXa Names coagulation factor IX
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173201.4	XP_005173258.1	coagulation factor IX isoform X1
XM_005173201.3	XP_005173258.1	coagulation factor IX isoform X1
NM_182868.2	NP_878288.2	coagulation factor IX precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	F9	NP_032005.1
Rattus norvegicus (Norway rat)	F9	NP_113728.1
	F9	NP_001103153.1
Gallus gallus (chicken)	F9	NP_989674.1
Danio rerio (zebrafish)	f9b	NP_001035400.1
Danio rerio (zebrafish)	f9a	NP_878288.1
Xenopus tropicalis (tropical clawed frog)	f9	NP_001011223.1
Homo sapiens (human)	F9	NP_000124.1
Pan troglodytes (chimpanzee)	F9	NP_001129063.1
Canis lupus familiaris (dog)	F9	NP_001003323.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

Insights into the evolutionary history of the vertebrate zic3 locus from a teleost-specific zic6 gene in the zebrafish, Danio rerio.
Keller MJ, Chitnis AB
Development genes and evolution217(7)541-7(2007 Jul)

Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.
Okuda Y, Yoda H, Uchikawa M, Furutani-Seiki M, Takeda H, Kondoh H, Kamachi Y
Developmental dynamics : an official publication of the American Association of Anatomists235(3)811-25(2006 Mar)

Comprehensive analysis of blood coagulation pathways in teleostei: evolution of coagulation factor genes and identification of zebrafish factor VIIi.
Hanumanthaiah R, Day K, Jagadeeswaran P
Blood cells, molecules & diseases29(1)57-68(2002 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following f9a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the f9a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40150	NM_182868.2 Latest version!	Danio rerio coagulation factor IXa (f9a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa19681	XM_005173201.4 Latest version!	Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa19681	XM_005173201.3	Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

f9a ( NM_182868.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_878288.2 Danio rerio coagulation factor IXa (f9a), mRNA.

Price & Availability↑

CloneID	ODa40150
Clone ID Related Accession (Same CDS sequence)	NM_182868.2
Accession Version	NM_182868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	coagulation factor IX precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004988.5, EH506608.1, EH490346.1, EH532764.1 and EH516696.1. On Jun 19, 2015 this sequence version replaced NM_182868.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01010022.1, AF515270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02418767, SAMN02418768 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG ATGGGAACCA GTGTAAACCA TCACCTTGTC AGAACCAGGG CACATGTGAG 
GACCAGATGG GCACATACAG CTGCAACTGT CTGCCAGGTT TTGTTGGCAA AAACTGTGAA 
ATAGTGACTG CCAAAAAGTG TGATGTTGAT AATGGTGGCT GTGAGCATTT CTGTGTTCTC 
CTGGAGTCTC ACAAGACCCA GTGTCAGTGT GCATATGGAT ACAAACTCTC AGAAGATGGA 
CTTACGTGTG AGCCAATAGT GAAGTTCCCA TGTGGCAAAA CCAGTAAACC TGCAGTGAAG 
ACCTATGGCA CAGCATCCGT GACGAATACA ACGGCTCAGT TTTCCAATAT GACTGTCAAA 
TCCACGTCCA GCATTCCCAA ACCAGACAAT CTGACAAACC CTACAAATTC CAAAGACATA 
AAAGAGAAAC TCGCGCCGCC ACGTGCTAAA CTGCCAAAAT GGGCCTTTGA GGAGTATTTG 
ACATCTCCTG CTCCTACAGT AAGTGGCCCA AAAAGTCGTA TAATTGGTGG CAATTCGGCC 
TTACCTGGAG AAATCCCTTG GCAGGTGGCG CTGGTGTCGC GCTCAACACA GCAGGTGTTC 
TGTGGAGGAT CCATCCTCAA CCCGCTGTGG GTGATCACTG CTGCCCACTG CCTGCTGGGA 
AACCACAACG GATCATTTTA CATCAGAGTT GGGGAGCATG ATGTCTCTAA AATTGAAGGC 
ACAGAACAGA ATGTGGACGT CATCAAATTA ATCTCACACC CTCGCTACAA CTCCAAAGTC 
AGCCTTTTCA ATCACGACAT CGCTTTGCTG CGGCTCCGCT CCCCCATTCG GTTAACTCCT 
ACGGTGCGTC CCATCTGCCT GGGCCCCATG GTCTTCTCCA ATACCCTGCT GCAGTCTGGA 
ACTCTGGCCA CAGTCAGCGG CTGGGGTCGT GTGCGCTTCC AGGGCCGAAG TGCAGCGACT 
TTACAGAAGA TTGAGTTGCC TTATGTGGAC AGAACAGTGT GTAAGGAAAG CAGCAGTGAC 
CCAATCACAC ACTTCATGTT CTGTGCCGGT CACTCGGACT CTCCTAAAGA TGCGTGTCAG 
GGGGACAGCG GAGGACCTCA TGTTATGCGC TACCACAACA CCTGGTTCCT CACTGGGATC 
ATCAGCTGGG GAGAGGAGTG TGCCAAGAAA GGAAAGTATG GTGTGTATAC ACAGGTTGGC 
AACTATTACC GGTGGATACA GCATACGATG GGGGTTACTA CAGGAGCCCT GCTAACTAAC 
GTGGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878288.2
CDS	91..1602
Translation	MASFILVLFPFLLLQAALGSVFLDGEKADSVLWRRRRANTWLFEEFLPGNVERECIEEKCVLEEAREAFENDEKTMEFWASYVDGNQCKPSPCQNQGTCEDQMGTYSCNCLPGFVGKNCEIVTAKKCDVDNGGCEHFCVLLESHKTQCQCAYGYKLSEDGLTCEPIVKFPCGKTSKPAVKTYGTASVTNTTAQFSNMTVKSTSSIPKPDNLTNPTNSKDIKEKLAPPRAKLPKWAFEEYLTSPAPTVSGPKSRIIGGNSALPGEIPWQVALVSRSTQQVFCGGSILNPLWVITAAHCLLGNHNGSFYIRVGEHDVSKIEGTEQNVDVIKLISHPRYNSKVSLFNHDIALLRLRSPIRLTPTVRPICLGPMVFSNTLLQSGTLATVSGWGRVRFQGRSAATLQKIELPYVDRTVCKESSSDPITHFMFCAGHSDSPKDACQGDSGGPHVMRYHNTWFLTGIISWGEECAKKGKYGVYTQVGNYYRWIQHTMGVTTGALLTNVDQ

Target ORF information:

RefSeq Version	NM_182868.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coagulation factor IXa (f9a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182868.2

ORF Insert Sequence:

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG ATGGGAACCA GTGTAAACCA TCACCTTGTC AGAACCAGGG CACATGTGAG 
GACCAGATGG GCACATACAG CTGCAACTGT CTGCCAGGTT TTGTTGGCAA AAACTGTGAA 
ATAGTGACTG CCAAAAAGTG TGATGTTGAT AATGGTGGCT GTGAGCATTT CTGTGTTCTC 
CTGGAGTCTC ACAAGACCCA GTGTCAGTGT GCATATGGAT ACAAACTCTC AGAAGATGGA 
CTTACGTGTG AGCCAATAGT GAAGTTCCCA TGTGGCAAAA CCAGTAAACC TGCAGTGAAG 
ACCTATGGCA CAGCATCCGT GACGAATACA ACGGCTCAGT TTTCCAATAT GACTGTCAAA 
TCCACGTCCA GCATTCCCAA ACCAGACAAT CTGACAAACC CTACAAATTC CAAAGACATA 
AAAGAGAAAC TCGCGCCGCC ACGTGCTAAA CTGCCAAAAT GGGCCTTTGA GGAGTATTTG 
ACATCTCCTG CTCCTACAGT AAGTGGCCCA AAAAGTCGTA TAATTGGTGG CAATTCGGCC 
TTACCTGGAG AAATCCCTTG GCAGGTGGCG CTGGTGTCGC GCTCAACACA GCAGGTGTTC 
TGTGGAGGAT CCATCCTCAA CCCGCTGTGG GTGATCACTG CTGCCCACTG CCTGCTGGGA 
AACCACAACG GATCATTTTA CATCAGAGTT GGGGAGCATG ATGTCTCTAA AATTGAAGGC 
ACAGAACAGA ATGTGGACGT CATCAAATTA ATCTCACACC CTCGCTACAA CTCCAAAGTC 
AGCCTTTTCA ATCACGACAT CGCTTTGCTG CGGCTCCGCT CCCCCATTCG GTTAACTCCT 
ACGGTGCGTC CCATCTGCCT GGGCCCCATG GTCTTCTCCA ATACCCTGCT GCAGTCTGGA 
ACTCTGGCCA CAGTCAGCGG CTGGGGTCGT GTGCGCTTCC AGGGCCGAAG TGCAGCGACT 
TTACAGAAGA TTGAGTTGCC TTATGTGGAC AGAACAGTGT GTAAGGAAAG CAGCAGTGAC 
CCAATCACAC ACTTCATGTT CTGTGCCGGT CACTCGGACT CTCCTAAAGA TGCGTGTCAG 
GGGGACAGCG GAGGACCTCA TGTTATGCGC TACCACAACA CCTGGTTCCT CACTGGGATC 
ATCAGCTGGG GAGAGGAGTG TGCCAAGAAA GGAAAGTATG GTGTGTATAC ACAGGTTGGC 
AACTATTACC GGTGGATACA GCATACGATG GGGGTTACTA CAGGAGCCCT GCTAACTAAC 
GTGGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

f9a ( XM_005173201.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173258.1 Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19681
Clone ID Related Accession (Same CDS sequence)	XM_005173201.4 , XM_005173201.3
Accession Version	XM_005173201.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	coagulation factor IX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173201.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG TGAAGTTCCC ATGTGGCAAA ACCAGTAAAC CTGCAGTGAA GACCTATGGC 
ACAGCATCCG TGACGAATAC AACGGCTCAG TTTTCCAATA TGACTGTCAA ATCCACGTCC 
AGCATTCCCA AACCAGACAA TCTGACAAAC CCTACAAATT CCAAAGACAT AAAAGAGAAA 
CTCGCGCCGC CACGTGCTAA ACTGCCAAAA TGGGCCTTTG AGGAGTATTT GACATCTCCT 
GCTCCTACAG TAAGTGGCCC AAAAAGTCGT ATAATTGGTG GCAATTCGGC CTTACCTGGA 
GAAATCCCTT GGCAGGTGGC GCTGGTGTCG CGCTCAACAC AGCAGGTGTT CTGTGGAGGA 
TCCATCCTCA ACCCGCTGTG GGTGATCACT GCTGCCCACT GCCTGCTGGG AAACCACAAC 
GGATCATTTT ACATCAGAGT TGGGGAGCAT GATGTCTCTA AAATTGAAGG CACAGAACAG 
AATGTGGACG TCATCAAATT AATCTCACAC CCTCGCTACA ACTCCAAAGT CAGCCTTTTC 
AATCACGACA TCGCTTTGCT GCGGCTCCGC TCCCCCATTC GGTTAACTCC TACGGTGCGT 
CCCATCTGCC TGGGCCCCAT GGTCTTCTCC AATACCCTGC TGCAGTCTGG AACTCTGGCC 
ACAGTCAGCG GCTGGGGTCG TGTGCGCTTC CAGGGCCGAA GTGCAGCGAC TTTACAGAAG 
ATTGAGTTGC CTTATGTGGA CAGAACAGTG TGTAAGGAAA GCAGCAGTGA CCCAATCACA 
CACTTCATGT TCTGTGCCGG TCACTCGGAC TCTCCTAAAG ATGCGTGTCA GGGGGACAGC 
GGAGGACCTC ATGTTATGCG CTACCACAAC ACCTGGTTCC TCACTGGGAT CATCAGCTGG 
GGAGAGGAGT GTGCCAAGAA AGGAAAGTAT GGTGTGTATA CACAGGTTGG CAACTATTAC 
CGGTGGATAC AGCATACGAT GGGGGTTACT ACAGGAGCCC TGCTAACTAA CGTGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173258.1
CDS	73..1335
Translation	MASFILVLFPFLLLQAALGSVFLDGEKADSVLWRRRRANTWLFEEFLPGNVERECIEEKCVLEEAREAFENDEKTMEFWASYVVKFPCGKTSKPAVKTYGTASVTNTTAQFSNMTVKSTSSIPKPDNLTNPTNSKDIKEKLAPPRAKLPKWAFEEYLTSPAPTVSGPKSRIIGGNSALPGEIPWQVALVSRSTQQVFCGGSILNPLWVITAAHCLLGNHNGSFYIRVGEHDVSKIEGTEQNVDVIKLISHPRYNSKVSLFNHDIALLRLRSPIRLTPTVRPICLGPMVFSNTLLQSGTLATVSGWGRVRFQGRSAATLQKIELPYVDRTVCKESSSDPITHFMFCAGHSDSPKDACQGDSGGPHVMRYHNTWFLTGIISWGEECAKKGKYGVYTQVGNYYRWIQHTMGVTTGALLTNVDQ

Target ORF information:

RefSeq Version	XM_005173201.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173201.4

ORF Insert Sequence:

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG TGAAGTTCCC ATGTGGCAAA ACCAGTAAAC CTGCAGTGAA GACCTATGGC 
ACAGCATCCG TGACGAATAC AACGGCTCAG TTTTCCAATA TGACTGTCAA ATCCACGTCC 
AGCATTCCCA AACCAGACAA TCTGACAAAC CCTACAAATT CCAAAGACAT AAAAGAGAAA 
CTCGCGCCGC CACGTGCTAA ACTGCCAAAA TGGGCCTTTG AGGAGTATTT GACATCTCCT 
GCTCCTACAG TAAGTGGCCC AAAAAGTCGT ATAATTGGTG GCAATTCGGC CTTACCTGGA 
GAAATCCCTT GGCAGGTGGC GCTGGTGTCG CGCTCAACAC AGCAGGTGTT CTGTGGAGGA 
TCCATCCTCA ACCCGCTGTG GGTGATCACT GCTGCCCACT GCCTGCTGGG AAACCACAAC 
GGATCATTTT ACATCAGAGT TGGGGAGCAT GATGTCTCTA AAATTGAAGG CACAGAACAG 
AATGTGGACG TCATCAAATT AATCTCACAC CCTCGCTACA ACTCCAAAGT CAGCCTTTTC 
AATCACGACA TCGCTTTGCT GCGGCTCCGC TCCCCCATTC GGTTAACTCC TACGGTGCGT 
CCCATCTGCC TGGGCCCCAT GGTCTTCTCC AATACCCTGC TGCAGTCTGG AACTCTGGCC 
ACAGTCAGCG GCTGGGGTCG TGTGCGCTTC CAGGGCCGAA GTGCAGCGAC TTTACAGAAG 
ATTGAGTTGC CTTATGTGGA CAGAACAGTG TGTAAGGAAA GCAGCAGTGA CCCAATCACA 
CACTTCATGT TCTGTGCCGG TCACTCGGAC TCTCCTAAAG ATGCGTGTCA GGGGGACAGC 
GGAGGACCTC ATGTTATGCG CTACCACAAC ACCTGGTTCC TCACTGGGAT CATCAGCTGG 
GGAGAGGAGT GTGCCAAGAA AGGAAAGTAT GGTGTGTATA CACAGGTTGG CAACTATTAC 
CGGTGGATAC AGCATACGAT GGGGGTTACT ACAGGAGCCC TGCTAACTAA CGTGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

f9a ( XM_005173201.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173258.1 Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19681
Clone ID Related Accession (Same CDS sequence)	XM_005173201.4 , XM_005173201.3
Accession Version	XM_005173201.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	coagulation factor IX isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG TGAAGTTCCC ATGTGGCAAA ACCAGTAAAC CTGCAGTGAA GACCTATGGC 
ACAGCATCCG TGACGAATAC AACGGCTCAG TTTTCCAATA TGACTGTCAA ATCCACGTCC 
AGCATTCCCA AACCAGACAA TCTGACAAAC CCTACAAATT CCAAAGACAT AAAAGAGAAA 
CTCGCGCCGC CACGTGCTAA ACTGCCAAAA TGGGCCTTTG AGGAGTATTT GACATCTCCT 
GCTCCTACAG TAAGTGGCCC AAAAAGTCGT ATAATTGGTG GCAATTCGGC CTTACCTGGA 
GAAATCCCTT GGCAGGTGGC GCTGGTGTCG CGCTCAACAC AGCAGGTGTT CTGTGGAGGA 
TCCATCCTCA ACCCGCTGTG GGTGATCACT GCTGCCCACT GCCTGCTGGG AAACCACAAC 
GGATCATTTT ACATCAGAGT TGGGGAGCAT GATGTCTCTA AAATTGAAGG CACAGAACAG 
AATGTGGACG TCATCAAATT AATCTCACAC CCTCGCTACA ACTCCAAAGT CAGCCTTTTC 
AATCACGACA TCGCTTTGCT GCGGCTCCGC TCCCCCATTC GGTTAACTCC TACGGTGCGT 
CCCATCTGCC TGGGCCCCAT GGTCTTCTCC AATACCCTGC TGCAGTCTGG AACTCTGGCC 
ACAGTCAGCG GCTGGGGTCG TGTGCGCTTC CAGGGCCGAA GTGCAGCGAC TTTACAGAAG 
ATTGAGTTGC CTTATGTGGA CAGAACAGTG TGTAAGGAAA GCAGCAGTGA CCCAATCACA 
CACTTCATGT TCTGTGCCGG TCACTCGGAC TCTCCTAAAG ATGCGTGTCA GGGGGACAGC 
GGAGGACCTC ATGTTATGCG CTACCACAAC ACCTGGTTCC TCACTGGGAT CATCAGCTGG 
GGAGAGGAGT GTGCCAAGAA AGGAAAGTAT GGTGTGTATA CACAGGTTGG CAACTATTAC 
CGGTGGATAC AGCATACGAT GGGGGTTACT ACAGGAGCCC TGCTAACTAA CGTGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173258.1
CDS	41..1303
Translation	MASFILVLFPFLLLQAALGSVFLDGEKADSVLWRRRRANTWLFEEFLPGNVERECIEEKCVLEEAREAFENDEKTMEFWASYVVKFPCGKTSKPAVKTYGTASVTNTTAQFSNMTVKSTSSIPKPDNLTNPTNSKDIKEKLAPPRAKLPKWAFEEYLTSPAPTVSGPKSRIIGGNSALPGEIPWQVALVSRSTQQVFCGGSILNPLWVITAAHCLLGNHNGSFYIRVGEHDVSKIEGTEQNVDVIKLISHPRYNSKVSLFNHDIALLRLRSPIRLTPTVRPICLGPMVFSNTLLQSGTLATVSGWGRVRFQGRSAATLQKIELPYVDRTVCKESSSDPITHFMFCAGHSDSPKDACQGDSGGPHVMRYHNTWFLTGIISWGEECAKKGKYGVYTQVGNYYRWIQHTMGVTTGALLTNVDQ

Target ORF information:

RefSeq Version	XM_005173201.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio coagulation factor IXa (f9a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173201.3

ORF Insert Sequence:

ATGGCTTCAT TCATTCTTGT TCTTTTCCCA TTTCTCCTGC TACAGGCAGC CCTAGGTTCT 
GTGTTCCTGG ACGGAGAGAA AGCTGACAGT GTGTTGTGGA GACGCAGACG AGCGAACACA 
TGGCTGTTTG AGGAGTTTTT GCCAGGCAAT GTGGAAAGGG AGTGTATAGA GGAGAAGTGT 
GTCCTGGAGG AGGCCAGAGA AGCCTTTGAA AATGACGAGA AGACAATGGA GTTTTGGGCA 
AGTTATGTTG TGAAGTTCCC ATGTGGCAAA ACCAGTAAAC CTGCAGTGAA GACCTATGGC 
ACAGCATCCG TGACGAATAC AACGGCTCAG TTTTCCAATA TGACTGTCAA ATCCACGTCC 
AGCATTCCCA AACCAGACAA TCTGACAAAC CCTACAAATT CCAAAGACAT AAAAGAGAAA 
CTCGCGCCGC CACGTGCTAA ACTGCCAAAA TGGGCCTTTG AGGAGTATTT GACATCTCCT 
GCTCCTACAG TAAGTGGCCC AAAAAGTCGT ATAATTGGTG GCAATTCGGC CTTACCTGGA 
GAAATCCCTT GGCAGGTGGC GCTGGTGTCG CGCTCAACAC AGCAGGTGTT CTGTGGAGGA 
TCCATCCTCA ACCCGCTGTG GGTGATCACT GCTGCCCACT GCCTGCTGGG AAACCACAAC 
GGATCATTTT ACATCAGAGT TGGGGAGCAT GATGTCTCTA AAATTGAAGG CACAGAACAG 
AATGTGGACG TCATCAAATT AATCTCACAC CCTCGCTACA ACTCCAAAGT CAGCCTTTTC 
AATCACGACA TCGCTTTGCT GCGGCTCCGC TCCCCCATTC GGTTAACTCC TACGGTGCGT 
CCCATCTGCC TGGGCCCCAT GGTCTTCTCC AATACCCTGC TGCAGTCTGG AACTCTGGCC 
ACAGTCAGCG GCTGGGGTCG TGTGCGCTTC CAGGGCCGAA GTGCAGCGAC TTTACAGAAG 
ATTGAGTTGC CTTATGTGGA CAGAACAGTG TGTAAGGAAA GCAGCAGTGA CCCAATCACA 
CACTTCATGT TCTGTGCCGG TCACTCGGAC TCTCCTAAAG ATGCGTGTCA GGGGGACAGC 
GGAGGACCTC ATGTTATGCG CTACCACAAC ACCTGGTTCC TCACTGGGAT CATCAGCTGG 
GGAGAGGAGT GTGCCAAGAA AGGAAAGTAT GGTGTGTATA CACAGGTTGG CAACTATTAC 
CGGTGGATAC AGCATACGAT GGGGGTTACT ACAGGAGCCC TGCTAACTAA CGTGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ

Insights into the evolutionary history of the vertebrate zic3 locus from a teleost-specific zic6 gene in the zebrafish, Danio rerio.
Development genes and evolution217(7)541-7(2007 Jul)
Keller MJ,Chitnis AB

Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.
Developmental dynamics : an official publication of the American Association of Anatomists235(3)811-25(2006 Mar)
Okuda Y,Yoda H,Uchikawa M,Furutani-Seiki M,Takeda H,Kondoh H,Kamachi Y

Comprehensive analysis of blood coagulation pathways in teleostei: evolution of coagulation factor genes and identification of zebrafish factor VIIi.
Blood cells, molecules & diseases29(1)57-68(2002 Jul-Aug)
Hanumanthaiah R,Day K,Jagadeeswaran P