smarca4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smarca4a
Entrez Gene ID	353295
Full Name	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a
Synonyms	brg1,smarca2,smarca4,wu:fb51a08
General protein information	Preferred Names SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a Names transcription activator BRG1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 brahma protein-like protein 1 brahma-related gene 1 yng young
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469728.1	XP_021325403.1	transcription activator BRG1 isoform X1
NM_181603.1	NP_853634.1	transcription activator BRG1

Pathways from BioSystems

Homologies

	SMARCA4	XP_002808217.1
Gallus gallus (chicken)	SMARCA4	NP_990390.1
Danio rerio (zebrafish)	smarca4a	NP_853634.1
Rattus norvegicus (Norway rat)	Smarca4	NP_599195.1
Homo sapiens (human)	SMARCA4	NP_001122321.1
Pan troglodytes (chimpanzee)	SMARCA4	XP_512384.4
Canis lupus familiaris (dog)	SMARCA4	XP_005632936.1
Bos taurus (cattle)	SMARCA4	NP_001099084.1
Mus musculus (house mouse)	Smarca4	NP_001167549.1

Gene Ontology
Bibliography

Related articles in PubMed

Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish.
Xiao C, Gao L, Hou Y, Xu C, Chang N, Wang F, Hu K, He A, Luo Y, Wang J, Peng J, Tang F, Zhu X, Xiong JW
Nature communications713787(2016 Dec)

The Zebrafish Equivalent of Alzheimer's Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress.
Ebrahimie E, Moussavi Nik SH, Newman M, Van Der Hoek M, Lardelli M
Journal of Alzheimer's disease : JAD52(2)581-608(2016 03)

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.
Lou X, Burrows JT, Scott IC
BMC developmental biology1541(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Jia XE, Ma K, Xu T, Gao L, Wu S, Fu C, Zhang W, Wang Z, Liu K, Dong M, Jing C, Ren C, Dong Z, Chen Y, Jin Y, Huang Q, Chang X, Deng M, Li L, Luo L, Zhu J, Dang Y, Chang HC, Zon LI, Zhou Y, Chen S, Pan W
Cell research25(8)946-62(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transplantation experiments demonstrated that med14 and brg1 are required directly in neural crest cells. Analysis of med14; brg1 double mutant embryos suggested the existence of strong genetic interaction between members of Mediator and BAF complexes.
Title: Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.

The purpose of this study was to develop a framework for analyzing retinal pigment epithelium (RPE) expression profiles from zebrafish smarca4 mutants.
Title: Expression profiling of the RPE in zebrafish smarca4 mutant revealed altered signals that potentially affect RPE and retinal differentiation.

These observations suggest a different regulatory mechanism on bhlhe22 expression by smarca4 and irx7.
Title: Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas.

Cellular expression of Smarca4 (Brg1)-regulated genes in zebrafish retinas
Title: Cellular expression of Smarca4 (Brg1)-regulated genes in zebrafish retinas.

Disrupting the balance between Brg1 and disease-causing cardiac transcription factors, including Tbx5, Tbx20 and Nkx2-5, causes severe cardiac anomalies.
Title: Chromatin remodelling complex dosage modulates transcription factor function in heart development.

The following smarca4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smarca4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01174	NM_181603.1 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa01174	XM_021469728.1 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smarca4a ( NM_181603.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_853634.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), mRNA.

Price & Availability↑

CloneID	ODa01174
Clone ID Related Accession (Same CDS sequence)	XM_021469728.1 , NM_181603.1
Accession Version	NM_181603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription activator BRG1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY205256.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY205256.1, BC163918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACTC CTGACCCACC CATGGGCGGG ACCCCTCGGC CGGGACCTTC CCCAGGCCCT 
GGGCCGTCTC CTGGGGCCAT GTTAGGGCCT AGTCCTGGAC CCTCACCTGG CTCTGCCCAT 
GGCATGATGG GCCCCAGCCC TGGGCCCCCC TCTTCGGGAC ACCCTCTACC TCCACAGGGC 
CCCTCAGGTT ACCCGCAGGA CAACATGCAC CAGATGCACA AGCCCATGGA GGGCATGCAT 
GAGAAAGGCA TTCCTGAAGA CCAGCGCTAT GGTCAGATGA AAGGAATGGG CATGAGGCAG 
GGTGGGCACA GCGGTATGGG ACCCCCACCC AGCCCTATGG ACCAACATTC TCAAGGTTAC 
CCATCTCCTC TGGGTGGTTC GGATCACGCC CCCAGCCCGG TACCTGCTAA TGGCCCACCA 
TCTGGCCCCA TGATGCCCTC TGGTCCTGGT GCTATGCCTA TGGAAGGCGG AGACCCGCAG 
GCAATGGCTC AACAGAACCG GAGTGGAGGT GCAGGTGGTG TTGCAGGGCC AGGACCCAGC 
GGAGGACCTC CAAACGCAGG TGGCCCTGGT GGTGCTGGCG GACCCACTCC TTTCAACCAA 
AACCAACTTC ATCAGCTGCG GGCACAGATC ATGGCCTATA AAATGCTTGC TCGCGGGCAG 
CCTTTACCAG ATCACCTACA GATGGCAGTG CAGGGAAAGC GACCCATGCC CGGCATGCAG 
CAGGGGATGC CCAACATGCC CCCTGCTTCA GGACAAGGAG CAGGACCTCC TGGACCTGGG 
GTTCCCGGTC CTGGTGGACC CAACTACAAT AGACCACATG GAATGGTTGG ACCAAACATG 
CCTCCTCCAG GACCCTCTGG TGTACCTCCA GGCCTGCAAG GACAACCCAC CAATGGACCA 
CCTAAACCTT GGCCTGAAGG ACCCATGGTG AACGCTGCTG CCCCCGCAAG TGCCCCACAG 
AAGCTGATAC CCCCTCAGCC CACAGGCCGC CCTTCTCCAG CACCACCGTC AGTTCCTCCC 
GCTGCCTCCC CTGTCATGCC CCCTCAGACC CAGTCTCCAG GTCAGCCAGC CCAGCCACCG 
ATGGTGCTGC ACCAGAAACA GAACCGCATC ACACCCATCC AGAAACCCCG GGGTCTAGAC 
CCGGTGGAGA TCCTCCAGGA AAGAGAGTAC AGGTTACAGG CACGTATTGC GCATCGTATT 
CAGGAACTGG AGAATCTTCC AGGCTCTCTG GCTGGAGACT TGAGGACCAA AGCCAACATT 
GAGCTCAAGG CACTTAGACT TCTGAACTTT CAGAGACAGC TCCGTCAGGA AGTGGTAGTG 
TGTATGCGGC GTGATACGGC CCTGGAGACT GCACTCAACG CCAAGGCCTA CAAGCGAAGT 
AAGAGACAGT CCCTGCGCGA GGCTCGTATC ACTGAGAAAC TGGAGAAACA GCAGAAGATC 
GAGCAGGAAC GCAAACGCAG ACAGAAACAC CAGGAGTATC TCAACAGTAT CCTGCAGCAT 
GCAAAGGACT TCAAGGAATA TCATCGTTCC ATCACCGCAA AGATGCAGAA GTTGACCAAA 
GCCGTAGCCA CGTACCACGC CAACACTGAG CGAGAGCAGA AGAAGGAGAA TGAGCGTATC 
GAGAAGGAGA GAATGAGAAG GCTTATGGCT GAAGATGAAG AGGGTTACAG GAAGCTGATC 
GATCAAAAGA AGGACAAACG TTTGGCGTAC CTGCTGCAGC AGACCGATGA GTATGTGGCC 
AATCTCACAG AGCTTGTCAG AGCTCACAAA GCAGCTCAGG CCCTCAAAGA CAAGAAGAAG 
AAGAAGAAAA AGAAGAAGCC AGAGAACGCT GAAGGTGGAG CTCCTGCGCT TGGTCCTGAT 
GGAGAGCCTC TGGATGAGAC TAGTCAAATG AGTGACCTGC CGGTGAAGGT GATTCACGTG 
GACAGTGGGA AGATCCTCAC TGGCATGGAC GCTCCTAAAG CTGGACAACT GGATACTTGG 
CTGGAGATGA ATCCAGGGTA CGAGGTGGCC CCGCGCTCAG ACAGTGAAGA TAGTGGCTCA 
GATGAGGAAG AGGATGATGA GGAAGAGCCT CAGCCCTCTC AGGCCCCGAC AGAGGAGAAG 
AAGGTGATTC CTGATCCAGA CAGCGAGGAT GTTTCTGAGG TTGATGCTCG TCACATTATT 
GAACATGCCA AGCAAGATGT AGACGATGAA TACGGGAACT CTGCATTCAT TCGGGGCCTG 
CAGTCATATT ATGCTGTGGC CCACGCCGTC ACAGAGAAGG TGGAAAAGCA ATCGAGTCTG 
CTAGTGAACG GGCAGCTCAA ACAATATCAG ATTAAAGGTC TGGAGTGGCT GGTGTCTCTG 
TATAACAATA ACCTAAATGG CATTCTGGCT GATGAAATGG GTTTGGGCAA AACTATCCAG 
ACCATCGCAC TCATCACCTA CCTCATGGAG TTCAAACGCC TCAACGGACC CTTCCTTATC 
ATCGTCCCCC TCTCGACTCT GTCGAACTGG GTGTACGAGT TTGATAAGTG GGCTCCATCT 
GTGGTGAAAG TTTCTTACAA GGGCTCTCCT GCTGCTCGCC GTGCGTTCCT TCCCATTTTG 
CGCAGTGGCA AATTCAACGT ACTGGTCACT ACTTATGAAT ACATCATCAA AGATAAGCAA 
GTGCTGGCCA AGCTCCGTTG GAAGTACATG ATCGTGGATG AAGGTCACCG CATGAAAAAT 
CACCACTGTA AACTGACTCA GGTATTAAAT ACGCACTACC TGGCACCCCG GCGTGTGCTG 
CTAACTGGCA CTCCACTGCA AAACAAGCTG CCCGAGCTGT GGGCGCTGCT CAACTTCCTG 
CTGCCCACCA TCTTCAAGAG CTGCAGCACC TTTGAGCAGT GGTTCAACGC GCCCTTCGCC 
ATGACTGGAG AGAAGGTGGA CCTAAATGAA GAAGAAACCA TCCTGATCAT CCGTCGTCTG 
CACAAAGTGC TTCGGCCCTT CCTGCTCAGG AGACTTAAGA AGGAAGTAGA GGCGCAGCTA 
CCAGAGAAGG TGGAATATGT GATCAAGTGC GACATGTCGG CTCTTCAAAG AGTGCTGTAC 
AGGCACATGC AGGCTAAAGG AGTGCTGCTC ACTGACGGCT CTGAGAAAGA CAAGAAGGGC 
AAAGGGGGCA CTAAGACACT GATGAACACT ATTATGCAGC TGAGGAAGAT CTGTAACCAC 
CCGTACATGT TTCAGCACAT TGAGGAATCT TTCTCTGAGC ACCTGGGCTT CACTGGAGGC 
ATCGTTCAGG GGTCTGATTT GTACCGTGCC TCTGGTAAAT TTGAGCTGCT GGACCGCATT 
CTGCCAAAGC TCCGCGCCAC CAACCACAAA GTGCTGCTCT TTTGTCAGAT GACCACACTC 
ATGACCATCA TGGAGGACTA CTTTGCTTAC CGCAACTTCA AATACCTCCG CTTAGACGGA 
ACCACTAAGG CTGAGGATCG TGGCATGTTG CTGAAGAACT TTAACGATCC TTCCCATCAG 
TACTTCATTT TCCTGCTGAG CACCAGAGCA GGGGGTTTGG GGCTGAACCT GCAGTCTGCC 
GACACTGTCA TCATCTTTGA CAGCGACTGG AACCCTCACC AGGATCTACA GGCTCAGGAT 
CGAGCCCATC GCATCGGGCA GCAGAATGAG GTGCGTGTGC TGCGTCTGTG CACTGTGAAC 
AGTGTGGAAG AAAAGATCTT GGCTGCTGCC AAATACAAAC TCAATGTGGA CCAGAAGGTC 
ATCCAAGCCG GTATGTTCGA CCAGAAGTCC TCCAGCCACG AGCGCAGAGC TTTCCTGCAG 
GCCATCCTGG AGCACGAAGA GCAGGATGAG GAGGAAGATG AAGTACCTGA TGATGAAACT 
GTTAATCAGA TGATCGCCAG AAGCGAGGAA GAGTTCGACC ACTTTATGCG CATGGATCTG 
GACAGGCGAC GTGAGGAGGC TCGTAACCCG AAGCGCAGGC CGAGGCTGAT GGAGGAAGAT 
GAGCTGCCCA CCTGGATCAT GAAGGACGAT GCTGAGGTGG AGAGGCTCAC CTGTGAAGAG 
GAAGAGGAGA AGATGTTCGG CCGTGGCTCC CGCCAGAGGA AGGAGGTGGA TTACAGTGAC 
TCTCTGACAG AGAAACAGTG GCTGAAGGCC ATCGAGGAAG GCACCCTGGA TGAGATTGAG 
GAGGAAGTGC GCCACAAGAA AACAACCCGG AAGAGGAAAC GTGACCGAGA TCTTGACCTT 
CCCGGTCCGG CCACACCTAG CTCCAGTGGG CGGAGCAGAG ATAAAGATGA CGACGGTAAG 
AAGCAGAAGA AACGCGGCCG CCCTCCTGCT GAGAAACTCT CACCCAATCC TCCATCACTA 
ACCAAGAAGA TGAAGAAGAC TGTGGACGCA GTGATCAAAT ACAAGGATGG CAATGGGAGG 
CAACTGAGCG AGGTGTTCAT TCAACTGCCG TCTCGTAAAG AGCTGCCCGA GTATTATGAA 
CTTATCCGCA AACCTGTGGA CTTCAGGAAG ATCAAGGAGC GCATCAGGAG TCACAAGTAC 
CGCAGTCTGA ATGACCTAGA GAAGGACGTT ATGCTGCTGT GTCAGAACGC TCAGACCTTC 
AACCTGGAGG GATCTTTGAT CTACGAGGAC TCTATCGTGC TGCAGTCTGT GTTCACCAGC 
GTGCGGCAGA AGATCGAGAA AGAGGAGGAA GAGAGTGAGG GAGATGACAG CGAGGAGGAA 
GAGGATGATG TCGATGAAGG ATCCGAGTCT GAATCTCGTT CAGTGAAGGT GAAGATAAAG 
TTGAGTAGAA AGGACAAGGC GGAGAGAGGA AAAGGACGAC GCCGAACCGG ACGCAGCTCG 
CGGCCTAAAC CTGTTGTTAG TGATGACGAC AGTGAGGAAG ACCAGGAAGA GGAGCATTCG 
GCTAGTGGCA GCGAGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853634.1
CDS	1..4884
Translation	MSTPDPPMGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHGMMGPSPGPPSSGHPLPPQGPSGYPQDNMHQMHKPMEGMHEKGIPEDQRYGQMKGMGMRQGGHSGMGPPPSPMDQHSQGYPSPLGGSDHAPSPVPANGPPSGPMMPSGPGAMPMEGGDPQAMAQQNRSGGAGGVAGPGPSGGPPNAGGPGGAGGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQGMPNMPPASGQGAGPPGPGVPGPGGPNYNRPHGMVGPNMPPPGPSGVPPGLQGQPTNGPPKPWPEGPMVNAAAPASAPQKLIPPQPTGRPSPAPPSVPPAASPVMPPQTQSPGQPAQPPMVLHQKQNRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKANIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSITAKMQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRAHKAAQALKDKKKKKKKKKPENAEGGAPALGPDGEPLDETSQMSDLPVKVIHVDSGKILTGMDAPKAGQLDTWLEMNPGYEVAPRSDSEDSGSDEEEDDEEEPQPSQAPTEEKKVIPDPDSEDVSEVDARHIIEHAKQDVDDEYGNSAFIRGLQSYYAVAHAVTEKVEKQSSLLVNGQLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEFKRLNGPFLIIVPLSTLSNWVYEFDKWAPSVVKVSYKGSPAARRAFLPILRSGKFNVLVTTYEYIIKDKQVLAKLRWKYMIVDEGHRMKNHHCKLTQVLNTHYLAPRRVLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGSDLYRASGKFELLDRILPKLRATNHKVLLFCQMTTLMTIMEDYFAYRNFKYLRLDGTTKAEDRGMLLKNFNDPSHQYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEEDEVPDDETVNQMIARSEEEFDHFMRMDLDRRREEARNPKRRPRLMEEDELPTWIMKDDAEVERLTCEEEEEKMFGRGSRQRKEVDYSDSLTEKQWLKAIEEGTLDEIEEEVRHKKTTRKRKRDRDLDLPGPATPSSSGRSRDKDDDGKKQKKRGRPPAEKLSPNPPSLTKKMKKTVDAVIKYKDGNGRQLSEVFIQLPSRKELPEYYELIRKPVDFRKIKERIRSHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEEEESEGDDSEEEEDDVDEGSESESRSVKVKIKLSRKDKAERGKGRRRTGRSSRPKPVVSDDDSEEDQEEEHSASGSEED

Target ORF information:

RefSeq Version	NM_181603.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181603.1

ORF Insert Sequence:

ATGTCCACTC CTGACCCACC CATGGGCGGG ACCCCTCGGC CGGGACCTTC CCCAGGCCCT 
GGGCCGTCTC CTGGGGCCAT GTTAGGGCCT AGTCCTGGAC CCTCACCTGG CTCTGCCCAT 
GGCATGATGG GCCCCAGCCC TGGGCCCCCC TCTTCGGGAC ACCCTCTACC TCCACAGGGC 
CCCTCAGGTT ACCCGCAGGA CAACATGCAC CAGATGCACA AGCCCATGGA GGGCATGCAT 
GAGAAAGGCA TTCCTGAAGA CCAGCGCTAT GGTCAGATGA AAGGAATGGG CATGAGGCAG 
GGTGGGCACA GCGGTATGGG ACCCCCACCC AGCCCTATGG ACCAACATTC TCAAGGTTAC 
CCATCTCCTC TGGGTGGTTC GGATCACGCC CCCAGCCCGG TACCTGCTAA TGGCCCACCA 
TCTGGCCCCA TGATGCCCTC TGGTCCTGGT GCTATGCCTA TGGAAGGCGG AGACCCGCAG 
GCAATGGCTC AACAGAACCG GAGTGGAGGT GCAGGTGGTG TTGCAGGGCC AGGACCCAGC 
GGAGGACCTC CAAACGCAGG TGGCCCTGGT GGTGCTGGCG GACCCACTCC TTTCAACCAA 
AACCAACTTC ATCAGCTGCG GGCACAGATC ATGGCCTATA AAATGCTTGC TCGCGGGCAG 
CCTTTACCAG ATCACCTACA GATGGCAGTG CAGGGAAAGC GACCCATGCC CGGCATGCAG 
CAGGGGATGC CCAACATGCC CCCTGCTTCA GGACAAGGAG CAGGACCTCC TGGACCTGGG 
GTTCCCGGTC CTGGTGGACC CAACTACAAT AGACCACATG GAATGGTTGG ACCAAACATG 
CCTCCTCCAG GACCCTCTGG TGTACCTCCA GGCCTGCAAG GACAACCCAC CAATGGACCA 
CCTAAACCTT GGCCTGAAGG ACCCATGGTG AACGCTGCTG CCCCCGCAAG TGCCCCACAG 
AAGCTGATAC CCCCTCAGCC CACAGGCCGC CCTTCTCCAG CACCACCGTC AGTTCCTCCC 
GCTGCCTCCC CTGTCATGCC CCCTCAGACC CAGTCTCCAG GTCAGCCAGC CCAGCCACCG 
ATGGTGCTGC ACCAGAAACA GAACCGCATC ACACCCATCC AGAAACCCCG GGGTCTAGAC 
CCGGTGGAGA TCCTCCAGGA AAGAGAGTAC AGGTTACAGG CACGTATTGC GCATCGTATT 
CAGGAACTGG AGAATCTTCC AGGCTCTCTG GCTGGAGACT TGAGGACCAA AGCCAACATT 
GAGCTCAAGG CACTTAGACT TCTGAACTTT CAGAGACAGC TCCGTCAGGA AGTGGTAGTG 
TGTATGCGGC GTGATACGGC CCTGGAGACT GCACTCAACG CCAAGGCCTA CAAGCGAAGT 
AAGAGACAGT CCCTGCGCGA GGCTCGTATC ACTGAGAAAC TGGAGAAACA GCAGAAGATC 
GAGCAGGAAC GCAAACGCAG ACAGAAACAC CAGGAGTATC TCAACAGTAT CCTGCAGCAT 
GCAAAGGACT TCAAGGAATA TCATCGTTCC ATCACCGCAA AGATGCAGAA GTTGACCAAA 
GCCGTAGCCA CGTACCACGC CAACACTGAG CGAGAGCAGA AGAAGGAGAA TGAGCGTATC 
GAGAAGGAGA GAATGAGAAG GCTTATGGCT GAAGATGAAG AGGGTTACAG GAAGCTGATC 
GATCAAAAGA AGGACAAACG TTTGGCGTAC CTGCTGCAGC AGACCGATGA GTATGTGGCC 
AATCTCACAG AGCTTGTCAG AGCTCACAAA GCAGCTCAGG CCCTCAAAGA CAAGAAGAAG 
AAGAAGAAAA AGAAGAAGCC AGAGAACGCT GAAGGTGGAG CTCCTGCGCT TGGTCCTGAT 
GGAGAGCCTC TGGATGAGAC TAGTCAAATG AGTGACCTGC CGGTGAAGGT GATTCACGTG 
GACAGTGGGA AGATCCTCAC TGGCATGGAC GCTCCTAAAG CTGGACAACT GGATACTTGG 
CTGGAGATGA ATCCAGGGTA CGAGGTGGCC CCGCGCTCAG ACAGTGAAGA TAGTGGCTCA 
GATGAGGAAG AGGATGATGA GGAAGAGCCT CAGCCCTCTC AGGCCCCGAC AGAGGAGAAG 
AAGGTGATTC CTGATCCAGA CAGCGAGGAT GTTTCTGAGG TTGATGCTCG TCACATTATT 
GAACATGCCA AGCAAGATGT AGACGATGAA TACGGGAACT CTGCATTCAT TCGGGGCCTG 
CAGTCATATT ATGCTGTGGC CCACGCCGTC ACAGAGAAGG TGGAAAAGCA ATCGAGTCTG 
CTAGTGAACG GGCAGCTCAA ACAATATCAG ATTAAAGGTC TGGAGTGGCT GGTGTCTCTG 
TATAACAATA ACCTAAATGG CATTCTGGCT GATGAAATGG GTTTGGGCAA AACTATCCAG 
ACCATCGCAC TCATCACCTA CCTCATGGAG TTCAAACGCC TCAACGGACC CTTCCTTATC 
ATCGTCCCCC TCTCGACTCT GTCGAACTGG GTGTACGAGT TTGATAAGTG GGCTCCATCT 
GTGGTGAAAG TTTCTTACAA GGGCTCTCCT GCTGCTCGCC GTGCGTTCCT TCCCATTTTG 
CGCAGTGGCA AATTCAACGT ACTGGTCACT ACTTATGAAT ACATCATCAA AGATAAGCAA 
GTGCTGGCCA AGCTCCGTTG GAAGTACATG ATCGTGGATG AAGGTCACCG CATGAAAAAT 
CACCACTGTA AACTGACTCA GGTATTAAAT ACGCACTACC TGGCACCCCG GCGTGTGCTG 
CTAACTGGCA CTCCACTGCA AAACAAGCTG CCCGAGCTGT GGGCGCTGCT CAACTTCCTG 
CTGCCCACCA TCTTCAAGAG CTGCAGCACC TTTGAGCAGT GGTTCAACGC GCCCTTCGCC 
ATGACTGGAG AGAAGGTGGA CCTAAATGAA GAAGAAACCA TCCTGATCAT CCGTCGTCTG 
CACAAAGTGC TTCGGCCCTT CCTGCTCAGG AGACTTAAGA AGGAAGTAGA GGCGCAGCTA 
CCAGAGAAGG TGGAATATGT GATCAAGTGC GACATGTCGG CTCTTCAAAG AGTGCTGTAC 
AGGCACATGC AGGCTAAAGG AGTGCTGCTC ACTGACGGCT CTGAGAAAGA CAAGAAGGGC 
AAAGGGGGCA CTAAGACACT GATGAACACT ATTATGCAGC TGAGGAAGAT CTGTAACCAC 
CCGTACATGT TTCAGCACAT TGAGGAATCT TTCTCTGAGC ACCTGGGCTT CACTGGAGGC 
ATCGTTCAGG GGTCTGATTT GTACCGTGCC TCTGGTAAAT TTGAGCTGCT GGACCGCATT 
CTGCCAAAGC TCCGCGCCAC CAACCACAAA GTGCTGCTCT TTTGTCAGAT GACCACACTC 
ATGACCATCA TGGAGGACTA CTTTGCTTAC CGCAACTTCA AATACCTCCG CTTAGACGGA 
ACCACTAAGG CTGAGGATCG TGGCATGTTG CTGAAGAACT TTAACGATCC TTCCCATCAG 
TACTTCATTT TCCTGCTGAG CACCAGAGCA GGGGGTTTGG GGCTGAACCT GCAGTCTGCC 
GACACTGTCA TCATCTTTGA CAGCGACTGG AACCCTCACC AGGATCTACA GGCTCAGGAT 
CGAGCCCATC GCATCGGGCA GCAGAATGAG GTGCGTGTGC TGCGTCTGTG CACTGTGAAC 
AGTGTGGAAG AAAAGATCTT GGCTGCTGCC AAATACAAAC TCAATGTGGA CCAGAAGGTC 
ATCCAAGCCG GTATGTTCGA CCAGAAGTCC TCCAGCCACG AGCGCAGAGC TTTCCTGCAG 
GCCATCCTGG AGCACGAAGA GCAGGATGAG GAGGAAGATG AAGTACCTGA TGATGAAACT 
GTTAATCAGA TGATCGCCAG AAGCGAGGAA GAGTTCGACC ACTTTATGCG CATGGATCTG 
GACAGGCGAC GTGAGGAGGC TCGTAACCCG AAGCGCAGGC CGAGGCTGAT GGAGGAAGAT 
GAGCTGCCCA CCTGGATCAT GAAGGACGAT GCTGAGGTGG AGAGGCTCAC CTGTGAAGAG 
GAAGAGGAGA AGATGTTCGG CCGTGGCTCC CGCCAGAGGA AGGAGGTGGA TTACAGTGAC 
TCTCTGACAG AGAAACAGTG GCTGAAGGCC ATCGAGGAAG GCACCCTGGA TGAGATTGAG 
GAGGAAGTGC GCCACAAGAA AACAACCCGG AAGAGGAAAC GTGACCGAGA TCTTGACCTT 
CCCGGTCCGG CCACACCTAG CTCCAGTGGG CGGAGCAGAG ATAAAGATGA CGACGGTAAG 
AAGCAGAAGA AACGCGGCCG CCCTCCTGCT GAGAAACTCT CACCCAATCC TCCATCACTA 
ACCAAGAAGA TGAAGAAGAC TGTGGACGCA GTGATCAAAT ACAAGGATGG CAATGGGAGG 
CAACTGAGCG AGGTGTTCAT TCAACTGCCG TCTCGTAAAG AGCTGCCCGA GTATTATGAA 
CTTATCCGCA AACCTGTGGA CTTCAGGAAG ATCAAGGAGC GCATCAGGAG TCACAAGTAC 
CGCAGTCTGA ATGACCTAGA GAAGGACGTT ATGCTGCTGT GTCAGAACGC TCAGACCTTC 
AACCTGGAGG GATCTTTGAT CTACGAGGAC TCTATCGTGC TGCAGTCTGT GTTCACCAGC 
GTGCGGCAGA AGATCGAGAA AGAGGAGGAA GAGAGTGAGG GAGATGACAG CGAGGAGGAA 
GAGGATGATG TCGATGAAGG ATCCGAGTCT GAATCTCGTT CAGTGAAGGT GAAGATAAAG 
TTGAGTAGAA AGGACAAGGC GGAGAGAGGA AAAGGACGAC GCCGAACCGG ACGCAGCTCG 
CGGCCTAAAC CTGTTGTTAG TGATGACGAC AGTGAGGAAG ACCAGGAAGA GGAGCATTCG 
GCTAGTGGCA GCGAGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smarca4a ( XM_021469728.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325403.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), transcript variant X1, mRNA.

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CloneID	ODa01174
Clone ID Related Accession (Same CDS sequence)	XM_021469728.1 , NM_181603.1
Accession Version	XM_021469728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription activator BRG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACTC CTGACCCACC CATGGGCGGG ACCCCTCGGC CGGGACCTTC CCCAGGCCCT 
GGGCCGTCTC CTGGGGCCAT GTTAGGGCCT AGTCCTGGAC CCTCACCTGG CTCTGCCCAT 
GGCATGATGG GCCCCAGCCC TGGGCCCCCC TCTTCGGGAC ACCCTCTACC TCCACAGGGC 
CCCTCAGGTT ACCCGCAGGA CAACATGCAC CAGATGCACA AGCCCATGGA GGGCATGCAT 
GAGAAAGGCA TTCCTGAAGA CCAGCGCTAT GGTCAGATGA AAGGAATGGG CATGAGGCAG 
GGTGGGCACA GCGGTATGGG ACCCCCACCC AGCCCTATGG ACCAACATTC TCAAGGTTAC 
CCATCTCCTC TGGGTGGTTC GGATCACGCC CCCAGCCCGG TACCTGCTAA TGGCCCACCA 
TCTGGCCCCA TGATGCCCTC TGGTCCTGGT GCTATGCCTA TGGAAGGCGG AGACCCGCAG 
GCAATGGCTC AACAGAACCG GAGTGGAGGT GCAGGTGGTG TTGCAGGGCC AGGACCCAGC 
GGAGGACCTC CAAACGCAGG TGGCCCTGGT GGTGCTGGCG GACCCACTCC TTTCAACCAA 
AACCAACTTC ATCAGCTGCG GGCACAGATC ATGGCCTATA AAATGCTTGC TCGCGGGCAG 
CCTTTACCAG ATCACCTACA GATGGCAGTG CAGGGAAAGC GACCCATGCC CGGCATGCAG 
CAGGGGATGC CCAACATGCC CCCTGCTTCA GGACAAGGAG CAGGACCTCC TGGACCTGGG 
GTTCCCGGTC CTGGTGGACC CAACTACAAT AGACCACATG GAATGGTTGG ACCAAACATG 
CCTCCTCCAG GACCCTCTGG TGTACCTCCA GGCCTGCAAG GACAACCCAC CAATGGACCA 
CCTAAACCTT GGCCTGAAGG ACCCATGGTG AACGCTGCTG CCCCCGCAAG TGCCCCACAG 
AAGCTGATAC CCCCTCAGCC CACAGGCCGC CCTTCTCCAG CACCACCGTC AGTTCCTCCC 
GCTGCCTCCC CTGTCATGCC CCCTCAGACC CAGTCTCCAG GTCAGCCAGC CCAGCCACCG 
ATGGTGCTGC ACCAGAAACA GAACCGCATC ACACCCATCC AGAAACCCCG GGGTCTAGAC 
CCGGTGGAGA TCCTCCAGGA AAGAGAGTAC AGGTTACAGG CACGTATTGC GCATCGTATT 
CAGGAACTGG AGAATCTTCC AGGCTCTCTG GCTGGAGACT TGAGGACCAA AGCCAACATT 
GAGCTCAAGG CACTTAGACT TCTGAACTTT CAGAGACAGC TCCGTCAGGA AGTGGTAGTG 
TGTATGCGGC GTGATACGGC CCTGGAGACT GCACTCAACG CCAAGGCCTA CAAGCGAAGT 
AAGAGACAGT CCCTGCGCGA GGCTCGTATC ACTGAGAAAC TGGAGAAACA GCAGAAGATC 
GAGCAGGAAC GCAAACGCAG ACAGAAACAC CAGGAGTATC TCAACAGTAT CCTGCAGCAT 
GCAAAGGACT TCAAGGAATA TCATCGTTCC ATCACCGCAA AGATGCAGAA GTTGACCAAA 
GCCGTAGCCA CGTACCACGC CAACACTGAG CGAGAGCAGA AGAAGGAGAA TGAGCGTATC 
GAGAAGGAGA GAATGAGAAG GCTTATGGCT GAAGATGAAG AGGGTTACAG GAAGCTGATC 
GATCAAAAGA AGGACAAACG TTTGGCGTAC CTGCTGCAGC AGACCGATGA GTATGTGGCC 
AATCTCACAG AGCTTGTCAG AGCTCACAAA GCAGCTCAGG CCCTCAAAGA CAAGAAGAAG 
AAGAAGAAAA AGAAGAAGCC AGAGAACGCT GAAGGTGGAG CTCCTGCGCT TGGTCCTGAT 
GGAGAGCCTC TGGATGAGAC TAGTCAAATG AGTGACCTGC CGGTGAAGGT GATTCACGTG 
GACAGTGGGA AGATCCTCAC TGGCATGGAC GCTCCTAAAG CTGGACAACT GGATACTTGG 
CTGGAGATGA ATCCAGGGTA CGAGGTGGCC CCGCGCTCAG ACAGTGAAGA TAGTGGCTCA 
GATGAGGAAG AGGATGATGA GGAAGAGCCT CAGCCCTCTC AGGCCCCGAC AGAGGAGAAG 
AAGGTGATTC CTGATCCAGA CAGCGAGGAT GTTTCTGAGG TTGATGCTCG TCACATTATT 
GAACATGCCA AGCAAGATGT AGACGATGAA TACGGGAACT CTGCATTCAT TCGGGGCCTG 
CAGTCATATT ATGCTGTGGC CCACGCCGTC ACAGAGAAGG TGGAAAAGCA ATCGAGTCTG 
CTAGTGAACG GGCAGCTCAA ACAATATCAG ATTAAAGGTC TGGAGTGGCT GGTGTCTCTG 
TATAACAATA ACCTAAATGG CATTCTGGCT GATGAAATGG GTTTGGGCAA AACTATCCAG 
ACCATCGCAC TCATCACCTA CCTCATGGAG TTCAAACGCC TCAACGGACC CTTCCTTATC 
ATCGTCCCCC TCTCGACTCT GTCGAACTGG GTGTACGAGT TTGATAAGTG GGCTCCATCT 
GTGGTGAAAG TTTCTTACAA GGGCTCTCCT GCTGCTCGCC GTGCGTTCCT TCCCATTTTG 
CGCAGTGGCA AATTCAACGT ACTGGTCACT ACTTATGAAT ACATCATCAA AGATAAGCAA 
GTGCTGGCCA AGCTCCGTTG GAAGTACATG ATCGTGGATG AAGGTCACCG CATGAAAAAT 
CACCACTGTA AACTGACTCA GGTATTAAAT ACGCACTACC TGGCACCCCG GCGTGTGCTG 
CTAACTGGCA CTCCACTGCA AAACAAGCTG CCCGAGCTGT GGGCGCTGCT CAACTTCCTG 
CTGCCCACCA TCTTCAAGAG CTGCAGCACC TTTGAGCAGT GGTTCAACGC GCCCTTCGCC 
ATGACTGGAG AGAAGGTGGA CCTAAATGAA GAAGAAACCA TCCTGATCAT CCGTCGTCTG 
CACAAAGTGC TTCGGCCCTT CCTGCTCAGG AGACTTAAGA AGGAAGTAGA GGCGCAGCTA 
CCAGAGAAGG TGGAATATGT GATCAAGTGC GACATGTCGG CTCTTCAAAG AGTGCTGTAC 
AGGCACATGC AGGCTAAAGG AGTGCTGCTC ACTGACGGCT CTGAGAAAGA CAAGAAGGGC 
AAAGGGGGCA CTAAGACACT GATGAACACT ATTATGCAGC TGAGGAAGAT CTGTAACCAC 
CCGTACATGT TTCAGCACAT TGAGGAATCT TTCTCTGAGC ACCTGGGCTT CACTGGAGGC 
ATCGTTCAGG GGTCTGATTT GTACCGTGCC TCTGGTAAAT TTGAGCTGCT GGACCGCATT 
CTGCCAAAGC TCCGCGCCAC CAACCACAAA GTGCTGCTCT TTTGTCAGAT GACCACACTC 
ATGACCATCA TGGAGGACTA CTTTGCTTAC CGCAACTTCA AATACCTCCG CTTAGACGGA 
ACCACTAAGG CTGAGGATCG TGGCATGTTG CTGAAGAACT TTAACGATCC TTCCCATCAG 
TACTTCATTT TCCTGCTGAG CACCAGAGCA GGGGGTTTGG GGCTGAACCT GCAGTCTGCC 
GACACTGTCA TCATCTTTGA CAGCGACTGG AACCCTCACC AGGATCTACA GGCTCAGGAT 
CGAGCCCATC GCATCGGGCA GCAGAATGAG GTGCGTGTGC TGCGTCTGTG CACTGTGAAC 
AGTGTGGAAG AAAAGATCTT GGCTGCTGCC AAATACAAAC TCAATGTGGA CCAGAAGGTC 
ATCCAAGCCG GTATGTTCGA CCAGAAGTCC TCCAGCCACG AGCGCAGAGC TTTCCTGCAG 
GCCATCCTGG AGCACGAAGA GCAGGATGAG GAGGAAGATG AAGTACCTGA TGATGAAACT 
GTTAATCAGA TGATCGCCAG AAGCGAGGAA GAGTTCGACC ACTTTATGCG CATGGATCTG 
GACAGGCGAC GTGAGGAGGC TCGTAACCCG AAGCGCAGGC CGAGGCTGAT GGAGGAAGAT 
GAGCTGCCCA CCTGGATCAT GAAGGACGAT GCTGAGGTGG AGAGGCTCAC CTGTGAAGAG 
GAAGAGGAGA AGATGTTCGG CCGTGGCTCC CGCCAGAGGA AGGAGGTGGA TTACAGTGAC 
TCTCTGACAG AGAAACAGTG GCTGAAGGCC ATCGAGGAAG GCACCCTGGA TGAGATTGAG 
GAGGAAGTGC GCCACAAGAA AACAACCCGG AAGAGGAAAC GTGACCGAGA TCTTGACCTT 
CCCGGTCCGG CCACACCTAG CTCCAGTGGG CGGAGCAGAG ATAAAGATGA CGACGGTAAG 
AAGCAGAAGA AACGCGGCCG CCCTCCTGCT GAGAAACTCT CACCCAATCC TCCATCACTA 
ACCAAGAAGA TGAAGAAGAC TGTGGACGCA GTGATCAAAT ACAAGGATGG CAATGGGAGG 
CAACTGAGCG AGGTGTTCAT TCAACTGCCG TCTCGTAAAG AGCTGCCCGA GTATTATGAA 
CTTATCCGCA AACCTGTGGA CTTCAGGAAG ATCAAGGAGC GCATCAGGAG TCACAAGTAC 
CGCAGTCTGA ATGACCTAGA GAAGGACGTT ATGCTGCTGT GTCAGAACGC TCAGACCTTC 
AACCTGGAGG GATCTTTGAT CTACGAGGAC TCTATCGTGC TGCAGTCTGT GTTCACCAGC 
GTGCGGCAGA AGATCGAGAA AGAGGAGGAA GAGAGTGAGG GAGATGACAG CGAGGAGGAA 
GAGGATGATG TCGATGAAGG ATCCGAGTCT GAATCTCGTT CAGTGAAGGT GAAGATAAAG 
TTGAGTAGAA AGGACAAGGC GGAGAGAGGA AAAGGACGAC GCCGAACCGG ACGCAGCTCG 
CGGCCTAAAC CTGTTGTTAG TGATGACGAC AGTGAGGAAG ACCAGGAAGA GGAGCATTCG 
GCTAGTGGCA GCGAGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325403.1
CDS	188..5071
Translation	MSTPDPPMGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHGMMGPSPGPPSSGHPLPPQGPSGYPQDNMHQMHKPMEGMHEKGIPEDQRYGQMKGMGMRQGGHSGMGPPPSPMDQHSQGYPSPLGGSDHAPSPVPANGPPSGPMMPSGPGAMPMEGGDPQAMAQQNRSGGAGGVAGPGPSGGPPNAGGPGGAGGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQGMPNMPPASGQGAGPPGPGVPGPGGPNYNRPHGMVGPNMPPPGPSGVPPGLQGQPTNGPPKPWPEGPMVNAAAPASAPQKLIPPQPTGRPSPAPPSVPPAASPVMPPQTQSPGQPAQPPMVLHQKQNRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKANIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSITAKMQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRAHKAAQALKDKKKKKKKKKPENAEGGAPALGPDGEPLDETSQMSDLPVKVIHVDSGKILTGMDAPKAGQLDTWLEMNPGYEVAPRSDSEDSGSDEEEDDEEEPQPSQAPTEEKKVIPDPDSEDVSEVDARHIIEHAKQDVDDEYGNSAFIRGLQSYYAVAHAVTEKVEKQSSLLVNGQLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEFKRLNGPFLIIVPLSTLSNWVYEFDKWAPSVVKVSYKGSPAARRAFLPILRSGKFNVLVTTYEYIIKDKQVLAKLRWKYMIVDEGHRMKNHHCKLTQVLNTHYLAPRRVLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGSDLYRASGKFELLDRILPKLRATNHKVLLFCQMTTLMTIMEDYFAYRNFKYLRLDGTTKAEDRGMLLKNFNDPSHQYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEEDEVPDDETVNQMIARSEEEFDHFMRMDLDRRREEARNPKRRPRLMEEDELPTWIMKDDAEVERLTCEEEEEKMFGRGSRQRKEVDYSDSLTEKQWLKAIEEGTLDEIEEEVRHKKTTRKRKRDRDLDLPGPATPSSSGRSRDKDDDGKKQKKRGRPPAEKLSPNPPSLTKKMKKTVDAVIKYKDGNGRQLSEVFIQLPSRKELPEYYELIRKPVDFRKIKERIRSHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEEEESEGDDSEEEEDDVDEGSESESRSVKVKIKLSRKDKAERGKGRRRTGRSSRPKPVVSDDDSEEDQEEEHSASGSEED

Target ORF information:

RefSeq Version	XM_021469728.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a (smarca4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469728.1

ORF Insert Sequence:

ATGTCCACTC CTGACCCACC CATGGGCGGG ACCCCTCGGC CGGGACCTTC CCCAGGCCCT 
GGGCCGTCTC CTGGGGCCAT GTTAGGGCCT AGTCCTGGAC CCTCACCTGG CTCTGCCCAT 
GGCATGATGG GCCCCAGCCC TGGGCCCCCC TCTTCGGGAC ACCCTCTACC TCCACAGGGC 
CCCTCAGGTT ACCCGCAGGA CAACATGCAC CAGATGCACA AGCCCATGGA GGGCATGCAT 
GAGAAAGGCA TTCCTGAAGA CCAGCGCTAT GGTCAGATGA AAGGAATGGG CATGAGGCAG 
GGTGGGCACA GCGGTATGGG ACCCCCACCC AGCCCTATGG ACCAACATTC TCAAGGTTAC 
CCATCTCCTC TGGGTGGTTC GGATCACGCC CCCAGCCCGG TACCTGCTAA TGGCCCACCA 
TCTGGCCCCA TGATGCCCTC TGGTCCTGGT GCTATGCCTA TGGAAGGCGG AGACCCGCAG 
GCAATGGCTC AACAGAACCG GAGTGGAGGT GCAGGTGGTG TTGCAGGGCC AGGACCCAGC 
GGAGGACCTC CAAACGCAGG TGGCCCTGGT GGTGCTGGCG GACCCACTCC TTTCAACCAA 
AACCAACTTC ATCAGCTGCG GGCACAGATC ATGGCCTATA AAATGCTTGC TCGCGGGCAG 
CCTTTACCAG ATCACCTACA GATGGCAGTG CAGGGAAAGC GACCCATGCC CGGCATGCAG 
CAGGGGATGC CCAACATGCC CCCTGCTTCA GGACAAGGAG CAGGACCTCC TGGACCTGGG 
GTTCCCGGTC CTGGTGGACC CAACTACAAT AGACCACATG GAATGGTTGG ACCAAACATG 
CCTCCTCCAG GACCCTCTGG TGTACCTCCA GGCCTGCAAG GACAACCCAC CAATGGACCA 
CCTAAACCTT GGCCTGAAGG ACCCATGGTG AACGCTGCTG CCCCCGCAAG TGCCCCACAG 
AAGCTGATAC CCCCTCAGCC CACAGGCCGC CCTTCTCCAG CACCACCGTC AGTTCCTCCC 
GCTGCCTCCC CTGTCATGCC CCCTCAGACC CAGTCTCCAG GTCAGCCAGC CCAGCCACCG 
ATGGTGCTGC ACCAGAAACA GAACCGCATC ACACCCATCC AGAAACCCCG GGGTCTAGAC 
CCGGTGGAGA TCCTCCAGGA AAGAGAGTAC AGGTTACAGG CACGTATTGC GCATCGTATT 
CAGGAACTGG AGAATCTTCC AGGCTCTCTG GCTGGAGACT TGAGGACCAA AGCCAACATT 
GAGCTCAAGG CACTTAGACT TCTGAACTTT CAGAGACAGC TCCGTCAGGA AGTGGTAGTG 
TGTATGCGGC GTGATACGGC CCTGGAGACT GCACTCAACG CCAAGGCCTA CAAGCGAAGT 
AAGAGACAGT CCCTGCGCGA GGCTCGTATC ACTGAGAAAC TGGAGAAACA GCAGAAGATC 
GAGCAGGAAC GCAAACGCAG ACAGAAACAC CAGGAGTATC TCAACAGTAT CCTGCAGCAT 
GCAAAGGACT TCAAGGAATA TCATCGTTCC ATCACCGCAA AGATGCAGAA GTTGACCAAA 
GCCGTAGCCA CGTACCACGC CAACACTGAG CGAGAGCAGA AGAAGGAGAA TGAGCGTATC 
GAGAAGGAGA GAATGAGAAG GCTTATGGCT GAAGATGAAG AGGGTTACAG GAAGCTGATC 
GATCAAAAGA AGGACAAACG TTTGGCGTAC CTGCTGCAGC AGACCGATGA GTATGTGGCC 
AATCTCACAG AGCTTGTCAG AGCTCACAAA GCAGCTCAGG CCCTCAAAGA CAAGAAGAAG 
AAGAAGAAAA AGAAGAAGCC AGAGAACGCT GAAGGTGGAG CTCCTGCGCT TGGTCCTGAT 
GGAGAGCCTC TGGATGAGAC TAGTCAAATG AGTGACCTGC CGGTGAAGGT GATTCACGTG 
GACAGTGGGA AGATCCTCAC TGGCATGGAC GCTCCTAAAG CTGGACAACT GGATACTTGG 
CTGGAGATGA ATCCAGGGTA CGAGGTGGCC CCGCGCTCAG ACAGTGAAGA TAGTGGCTCA 
GATGAGGAAG AGGATGATGA GGAAGAGCCT CAGCCCTCTC AGGCCCCGAC AGAGGAGAAG 
AAGGTGATTC CTGATCCAGA CAGCGAGGAT GTTTCTGAGG TTGATGCTCG TCACATTATT 
GAACATGCCA AGCAAGATGT AGACGATGAA TACGGGAACT CTGCATTCAT TCGGGGCCTG 
CAGTCATATT ATGCTGTGGC CCACGCCGTC ACAGAGAAGG TGGAAAAGCA ATCGAGTCTG 
CTAGTGAACG GGCAGCTCAA ACAATATCAG ATTAAAGGTC TGGAGTGGCT GGTGTCTCTG 
TATAACAATA ACCTAAATGG CATTCTGGCT GATGAAATGG GTTTGGGCAA AACTATCCAG 
ACCATCGCAC TCATCACCTA CCTCATGGAG TTCAAACGCC TCAACGGACC CTTCCTTATC 
ATCGTCCCCC TCTCGACTCT GTCGAACTGG GTGTACGAGT TTGATAAGTG GGCTCCATCT 
GTGGTGAAAG TTTCTTACAA GGGCTCTCCT GCTGCTCGCC GTGCGTTCCT TCCCATTTTG 
CGCAGTGGCA AATTCAACGT ACTGGTCACT ACTTATGAAT ACATCATCAA AGATAAGCAA 
GTGCTGGCCA AGCTCCGTTG GAAGTACATG ATCGTGGATG AAGGTCACCG CATGAAAAAT 
CACCACTGTA AACTGACTCA GGTATTAAAT ACGCACTACC TGGCACCCCG GCGTGTGCTG 
CTAACTGGCA CTCCACTGCA AAACAAGCTG CCCGAGCTGT GGGCGCTGCT CAACTTCCTG 
CTGCCCACCA TCTTCAAGAG CTGCAGCACC TTTGAGCAGT GGTTCAACGC GCCCTTCGCC 
ATGACTGGAG AGAAGGTGGA CCTAAATGAA GAAGAAACCA TCCTGATCAT CCGTCGTCTG 
CACAAAGTGC TTCGGCCCTT CCTGCTCAGG AGACTTAAGA AGGAAGTAGA GGCGCAGCTA 
CCAGAGAAGG TGGAATATGT GATCAAGTGC GACATGTCGG CTCTTCAAAG AGTGCTGTAC 
AGGCACATGC AGGCTAAAGG AGTGCTGCTC ACTGACGGCT CTGAGAAAGA CAAGAAGGGC 
AAAGGGGGCA CTAAGACACT GATGAACACT ATTATGCAGC TGAGGAAGAT CTGTAACCAC 
CCGTACATGT TTCAGCACAT TGAGGAATCT TTCTCTGAGC ACCTGGGCTT CACTGGAGGC 
ATCGTTCAGG GGTCTGATTT GTACCGTGCC TCTGGTAAAT TTGAGCTGCT GGACCGCATT 
CTGCCAAAGC TCCGCGCCAC CAACCACAAA GTGCTGCTCT TTTGTCAGAT GACCACACTC 
ATGACCATCA TGGAGGACTA CTTTGCTTAC CGCAACTTCA AATACCTCCG CTTAGACGGA 
ACCACTAAGG CTGAGGATCG TGGCATGTTG CTGAAGAACT TTAACGATCC TTCCCATCAG 
TACTTCATTT TCCTGCTGAG CACCAGAGCA GGGGGTTTGG GGCTGAACCT GCAGTCTGCC 
GACACTGTCA TCATCTTTGA CAGCGACTGG AACCCTCACC AGGATCTACA GGCTCAGGAT 
CGAGCCCATC GCATCGGGCA GCAGAATGAG GTGCGTGTGC TGCGTCTGTG CACTGTGAAC 
AGTGTGGAAG AAAAGATCTT GGCTGCTGCC AAATACAAAC TCAATGTGGA CCAGAAGGTC 
ATCCAAGCCG GTATGTTCGA CCAGAAGTCC TCCAGCCACG AGCGCAGAGC TTTCCTGCAG 
GCCATCCTGG AGCACGAAGA GCAGGATGAG GAGGAAGATG AAGTACCTGA TGATGAAACT 
GTTAATCAGA TGATCGCCAG AAGCGAGGAA GAGTTCGACC ACTTTATGCG CATGGATCTG 
GACAGGCGAC GTGAGGAGGC TCGTAACCCG AAGCGCAGGC CGAGGCTGAT GGAGGAAGAT 
GAGCTGCCCA CCTGGATCAT GAAGGACGAT GCTGAGGTGG AGAGGCTCAC CTGTGAAGAG 
GAAGAGGAGA AGATGTTCGG CCGTGGCTCC CGCCAGAGGA AGGAGGTGGA TTACAGTGAC 
TCTCTGACAG AGAAACAGTG GCTGAAGGCC ATCGAGGAAG GCACCCTGGA TGAGATTGAG 
GAGGAAGTGC GCCACAAGAA AACAACCCGG AAGAGGAAAC GTGACCGAGA TCTTGACCTT 
CCCGGTCCGG CCACACCTAG CTCCAGTGGG CGGAGCAGAG ATAAAGATGA CGACGGTAAG 
AAGCAGAAGA AACGCGGCCG CCCTCCTGCT GAGAAACTCT CACCCAATCC TCCATCACTA 
ACCAAGAAGA TGAAGAAGAC TGTGGACGCA GTGATCAAAT ACAAGGATGG CAATGGGAGG 
CAACTGAGCG AGGTGTTCAT TCAACTGCCG TCTCGTAAAG AGCTGCCCGA GTATTATGAA 
CTTATCCGCA AACCTGTGGA CTTCAGGAAG ATCAAGGAGC GCATCAGGAG TCACAAGTAC 
CGCAGTCTGA ATGACCTAGA GAAGGACGTT ATGCTGCTGT GTCAGAACGC TCAGACCTTC 
AACCTGGAGG GATCTTTGAT CTACGAGGAC TCTATCGTGC TGCAGTCTGT GTTCACCAGC 
GTGCGGCAGA AGATCGAGAA AGAGGAGGAA GAGAGTGAGG GAGATGACAG CGAGGAGGAA 
GAGGATGATG TCGATGAAGG ATCCGAGTCT GAATCTCGTT CAGTGAAGGT GAAGATAAAG 
TTGAGTAGAA AGGACAAGGC GGAGAGAGGA AAAGGACGAC GCCGAACCGG ACGCAGCTCG 
CGGCCTAAAC CTGTTGTTAG TGATGACGAC AGTGAGGAAG ACCAGGAAGA GGAGCATTCG 
GCTAGTGGCA GCGAGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish.
Nature communications713787(2016 Dec)
Xiao C,Gao L,Hou Y,Xu C,Chang N,Wang F,Hu K,He A,Luo Y,Wang J,Peng J,Tang F,Zhu X,Xiong JW

The Zebrafish Equivalent of Alzheimer's Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress.
Journal of Alzheimer's disease : JAD52(2)581-608(2016 03)
Ebrahimie E,Moussavi Nik SH,Newman M,Van Der Hoek M,Lardelli M

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.
BMC developmental biology1541(2015 Nov)
Lou X,Burrows JT,Scott IC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Cell research25(8)946-62(2015 Aug)
Jia XE,Ma K,Xu T,Gao L,Wu S,Fu C,Zhang W,Wang Z,Liu K,Dong M,Jing C,Ren C,Dong Z,Chen Y,Jin Y,Huang Q,Chang X,Deng M,Li L,Luo L,Zhu J,Dang Y,Chang HC,Zon LI,Zhou Y,Chen S,Pan W

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smarca4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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