gpx4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpx4a
Entrez Gene ID	352928
Full Name	glutathione peroxidase 4a
Synonyms	PHGPxa,cb563,cb691,sb:cb563,wu:fb82e03,zgc:92253
General protein information	Preferred Names glutathione peroxidase 4a Names glutathione peroxidase 4a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development. This isozyme is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. A paralog of this gene is present on chromosome 2. Each paralog has a distinct pattern of expression during embryonic development. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007282.2	NP_001007283.2	glutathione peroxidase 4a isoform 2 precursor
NM_001346537.1	NP_001333466.1	glutathione peroxidase 4a isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPX4	NP_001139295.1
Caenorhabditis elegans (roundworm)	F26E4.12	NP_492598.1
Arabidopsis thaliana (thale cress)	GPX6	NP_192897.2
Oryza sativa (rice)	Os02g0664000	NP_001047659.1
Danio rerio (zebrafish)	gpx4a	NP_001007283.2
Drosophila melanogaster (fruit fly)	PHGPx	NP_728869.1
Anopheles gambiae (African malaria mosquito)	GPXH1	XP_003436755.1
Caenorhabditis elegans (roundworm)	R05H10.5	NP_497078.1
Oryza sativa (rice)	Os04g0556300	NP_001053524.1

Gene Ontology
Bibliography

Related articles in PubMed

Transforming growth factor-?1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Zhang CY, Yin HM, Wang H, Su D, Xia Y, Yan LF, Fang B, Liu W, Wang YM, Gu AH, Zhou Y
Leukemia(2017 Jun)

Glutathione peroxidase 4 inhibits Wnt/?-catenin signaling and regulates dorsal organizer formation in zebrafish embryos.
Rong X, Zhou Y, Liu Y, Zhao B, Wang B, Wang C, Gong X, Tang P, Lu L, Li Y, Zhao C, Zhou J
Development (Cambridge, England)144(9)1687-1697(2017 05)

Phenotypically anchored transcriptome profiling of developmental exposure to the antimicrobial agent, triclosan, reveals hepatotoxicity in embryonic zebrafish.
Haggard DE, Noyes PD, Waters KM, Tanguay RL
Toxicology and applied pharmacology30832-45(2016 10)

Spatial and temporal expression of zebrafish glutathione peroxidase 4 a and b genes during early embryo development.
Mendieta-Serrano MA, Schnabel D, Lomel? H, Salas-Vidal E
Gene expression patterns : GEP19(1-2)98-107(2015 Sep-Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that the gpx4b gene shows maternal and zygotic gene expression in the embryo proper compared to gpx4a that showed zygotic gene expression in the periderm covering the yolk cell only.
Title: Spatial and temporal expression of zebrafish glutathione peroxidase 4 a and b genes during early embryo development.

The following gpx4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpx4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00260	NM_001007282.2 Latest version!	Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa98464	NM_001346537.1 Latest version!	Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpx4a ( NM_001007282.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007283.2 Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa00260
Clone ID Related Accession (Same CDS sequence)	NM_001007282.2
Accession Version	NM_001007282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	glutathione peroxidase 4a isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH582507.1 and EH584097.1. On Oct 18, 2016 this sequence version replaced NM_001007282.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and uses an alternate translation start site compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EH584097.1, CN329699.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505372, SAMEA3505392 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTTTCT TAGGGTCTGC TGTCGTTTTC TCTCTAGTGT TGCAGACTAT GTCTGCACAG 
CTAGAGGACT GGCAGACGGC CAAATCCATT TATGAATTCA CAGCCACAGA TATAGATGGA 
AATGAAGTTT CCCTGGAAAA GTACAGGGGA AAAGTTGTGA TCATCACAAA CGTTGCCTCC 
AAATGAGGTA AAACCCCAGT AAACTACTCT CAGTTTGCAG AGATGCACGC CAAGTACTCT 
GAGAGAGGTT TACGCATCCT GGCTTTCCCT TCCAACCAGT TCGGACGTCA GGAACCAGGA 
ACTAATTCCC AAATCAAGGA ATTCGCCAAG TCATACAATG CAGAGTTTGA CATGTTCAGC 
AAGATTGATG TGAATGGCGA CGGGGCTCAT CCGCTCTGGA AGTGGCTGAA GGATCAGCCT 
AATGGAAAAG GATTCCTTGG AAATGGCATC AAATGGAATT TCACAAAGTT CCTTATTAAC 
CGTGAAGGCC AGGTTGTGAA GAGATACTCC CCACTGCAGG ATCCAAGTGT GGTGGAGAAG 
GATCTTTCTA AATATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007283.2
CDS	75..635
Translation	MRFLGSAVVFSLVLQTMSAQLEDWQTAKSIYEFTATDIDGNEVSLEKYRGKVVIITNVASK*GKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQEPGTNSQIKEFAKSYNAEFDMFSKIDVNGDGAHPLWKWLKDQPNGKGFLGNGIKWNFTKFLINREGQVVKRYSPLQDPSVVEKDLSKYL

Target ORF information:

RefSeq Version	NM_001007282.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007282.2

ORF Insert Sequence:

ATGCGTTTCT TAGGGTCTGC TGTCGTTTTC TCTCTAGTGT TGCAGACTAT GTCTGCACAG 
CTAGAGGACT GGCAGACGGC CAAATCCATT TATGAATTCA CAGCCACAGA TATAGATGGA 
AATGAAGTTT CCCTGGAAAA GTACAGGGGA AAAGTTGTGA TCATCACAAA CGTTGCCTCC 
AAATGAGGTA AAACCCCAGT AAACTACTCT CAGTTTGCAG AGATGCACGC CAAGTACTCT 
GAGAGAGGTT TACGCATCCT GGCTTTCCCT TCCAACCAGT TCGGACGTCA GGAACCAGGA 
ACTAATTCCC AAATCAAGGA ATTCGCCAAG TCATACAATG CAGAGTTTGA CATGTTCAGC 
AAGATTGATG TGAATGGCGA CGGGGCTCAT CCGCTCTGGA AGTGGCTGAA GGATCAGCCT 
AATGGAAAAG GATTCCTTGG AAATGGCATC AAATGGAATT TCACAAAGTT CCTTATTAAC 
CGTGAAGGCC AGGTTGTGAA GAGATACTCC CCACTGCAGG ATCCAAGTGT GGTGGAGAAG 
GATCTTTCTA AATATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpx4a ( NM_001346537.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001333466.1 Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa98464
Clone ID Related Accession (Same CDS sequence)	NM_001346537.1
Accession Version	NM_001346537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	glutathione peroxidase 4a isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH454234.1 and EH584097.1. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EH594416.1, EH463305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476741, SAMEA4476757 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAA TGGGGTTCAT CCACCGTTTT TTGCTGTTCG GGGCTTTATC GAGCAGTGGG 
ATCATTGGTG CAACGTCTGC ACAGCTAGAG GACTGGCAGA CGGCCAAATC CATTTATGAA 
TTCACAGCCA CAGATATAGA TGGAAATGAA GTTTCCCTGG AAAAGTACAG GGGAAAAGTT 
GTGATCATCA CAAACGTTGC CTCCAAATGA GGTAAAACCC CAGTAAACTA CTCTCAGTTT 
GCAGAGATGC ACGCCAAGTA CTCTGAGAGA GGTTTACGCA TCCTGGCTTT CCCTTCCAAC 
CAGTTCGGAC GTCAGGAACC AGGAACTAAT TCCCAAATCA AGGAATTCGC CAAGTCATAC 
AATGCAGAGT TTGACATGTT CAGCAAGATT GATGTGAATG GCGACGGGGC TCATCCGCTC 
TGGAAGTGGC TGAAGGATCA GCCTAATGGA AAAGGATTCC TTGGAAATGG CATCAAATGG 
AATTTCACAA AGTTCCTTAT TAACCGTGAA GGCCAGGTTG TGAAGAGATA CTCCCCACTG 
CAGGATCCAA GTGTGGTGGA GAAGGATCTT TCTAAATATC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333466.1
CDS	59..643
Translation	MQKMGFIHRFLLFGALSSSGIIGATSAQLEDWQTAKSIYEFTATDIDGNEVSLEKYRGKVVIITNVASK*GKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQEPGTNSQIKEFAKSYNAEFDMFSKIDVNGDGAHPLWKWLKDQPNGKGFLGNGIKWNFTKFLINREGQVVKRYSPLQDPSVVEKDLSKYL

Target ORF information:

RefSeq Version	NM_001346537.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutathione peroxidase 4a (gpx4a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346537.1

ORF Insert Sequence:

ATGCAGAAAA TGGGGTTCAT CCACCGTTTT TTGCTGTTCG GGGCTTTATC GAGCAGTGGG 
ATCATTGGTG CAACGTCTGC ACAGCTAGAG GACTGGCAGA CGGCCAAATC CATTTATGAA 
TTCACAGCCA CAGATATAGA TGGAAATGAA GTTTCCCTGG AAAAGTACAG GGGAAAAGTT 
GTGATCATCA CAAACGTTGC CTCCAAATGA GGTAAAACCC CAGTAAACTA CTCTCAGTTT 
GCAGAGATGC ACGCCAAGTA CTCTGAGAGA GGTTTACGCA TCCTGGCTTT CCCTTCCAAC 
CAGTTCGGAC GTCAGGAACC AGGAACTAAT TCCCAAATCA AGGAATTCGC CAAGTCATAC 
AATGCAGAGT TTGACATGTT CAGCAAGATT GATGTGAATG GCGACGGGGC TCATCCGCTC 
TGGAAGTGGC TGAAGGATCA GCCTAATGGA AAAGGATTCC TTGGAAATGG CATCAAATGG 
AATTTCACAA AGTTCCTTAT TAACCGTGAA GGCCAGGTTG TGAAGAGATA CTCCCCACTG 
CAGGATCCAA GTGTGGTGGA GAAGGATCTT TCTAAATATC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transforming growth factor-?1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Leukemia(2017 Jun)
Zhang CY,Yin HM,Wang H,Su D,Xia Y,Yan LF,Fang B,Liu W,Wang YM,Gu AH,Zhou Y

Glutathione peroxidase 4 inhibits Wnt/?-catenin signaling and regulates dorsal organizer formation in zebrafish embryos.
Development (Cambridge, England)144(9)1687-1697(2017 05)
Rong X,Zhou Y,Liu Y,Zhao B,Wang B,Wang C,Gong X,Tang P,Lu L,Li Y,Zhao C,Zhou J

Phenotypically anchored transcriptome profiling of developmental exposure to the antimicrobial agent, triclosan, reveals hepatotoxicity in embryonic zebrafish.
Toxicology and applied pharmacology30832-45(2016 10)
Haggard DE,Noyes PD,Waters KM,Tanguay RL

Spatial and temporal expression of zebrafish glutathione peroxidase 4 a and b genes during early embryo development.
Gene expression patterns : GEP19(1-2)98-107(2015 Sep-Nov)
Mendieta-Serrano MA,Schnabel D,Lomel? H,Salas-Vidal E

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gpx4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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