selenot1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenot1a
Entrez Gene ID	352919
Full Name	selenoprotein T, 1a
Synonyms	cb700,selt1a,sept1a,wu:fb63h09,wu:fc79a12
General protein information	Preferred Names selenoprotein T, 1a Names thioredoxin reductase-like selenoprotein T1a thioredoxin reductase-like enzyme
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs in vertebrates contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Paralogs of this gene are found on chromosomes 18 (GeneID:352921) and 14 (GeneID:352917). [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178290.6	NP_840075.2	thioredoxin reductase-like selenoprotein T1a precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SELT	NP_001096573.2
Mus musculus (house mouse)	Selt	NP_001035486.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009202	XP_319979.4
Canis lupus familiaris (dog)	SELT	NP_001157959.1
Homo sapiens (human)	SELT	NP_057359.2
Danio rerio (zebrafish)	selt1a	NP_840075.2
Rattus norvegicus (Norway rat)	Selt	NP_001014275.2
Gallus gallus (chicken)	SELT	NP_001006557.3
Xenopus tropicalis (tropical clawed frog)	selt	NP_988868.2
	SELT	NP_001152886.1
Drosophila melanogaster (fruit fly)	CG3887	NP_608897.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Hsu CH, Lin JS, Po Lai K, Li JW, Chan TF, You MS, Tse WK, Jiang YJ
Scientific reports510673(2015 Jun)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenot1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenot1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38888	NM_178290.6 Latest version!	Danio rerio selenoprotein T, 1a (selenot1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenot1a ( NM_178290.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840075.2 Danio rerio selenoprotein T, 1a (selenot1a), mRNA.

Price & Availability↑

CloneID	ODa38888
Clone ID Related Accession (Same CDS sequence)	NM_178290.6
Accession Version	NM_178290.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Danio rerio(zebrafish)
Product	thioredoxin reductase-like selenoprotein T1a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB359649.1, EH438162.1, BC054578.1 and EH473273.1. On Feb 3, 2010 this sequence version replaced NM_178290.5. ##Evidence-Data-START## Transcript exon combination :: BC054578.1, EH550504.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGA TGCGCTGGCT GCCGTTCTCG GCTCTACTAT TATGGGCCTT ATGTCTTCAC 
AGCGCTTCTG CCGATAACAA TGGCGTGAAG AAAATGAAGA TGCAGTTTGC CACGGGTCCA 
CTTCTCAAAT TCCAGATTTG TGTCTCCTGA GGGTACAAGC GGGTGTTTGA GGAGTACACG 
CAGGCTTTGT ACCAGCGGTA CCCAGACATC CGCATAGAGG GAGAGAACTA CCTTCCTCTG 
CCCCTCTACA GGCACATTGC TTCCTTTCTG TCAATGTTTA AGCTGTTGTT AATCGGAGTG 
ATCATCTTGG GCAAGGATCC TTTTGCTTTA TGTGGCATGC AGGCTCCAGG GATCTGGGTT 
TGGAGTCAGG AGAATAAGAT ATATGCTTGT ATGATGGTGT TCTTCTTCAG CAATATGATC 
GAAAATCAGT GCATGTCTAC AGGCGCATTT GAAATCACGC TCAATGATGT CCCAGTTTGG 
TCCAAGCTGG AGTCAGGACA CCTGCCTTCA ATGCAGCAGC TAGTCCAAAT CCTGGAGAAT 
GAGATGAAGA TGAGCATGCA CATGGACACA CTTCCACCCC ATCAGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840075.2
CDS	49..639
Translation	MGKMRWLPFSALLLWALCLHSASADNNGVKKMKMQFATGPLLKFQICVS*GYKRVFEEYTQALYQRYPDIRIEGENYLPLPLYRHIASFLSMFKLLLIGVIILGKDPFALCGMQAPGIWVWSQENKIYACMMVFFFSNMIENQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILENEMKMSMHMDTLPPHQS

Target ORF information:

RefSeq Version	NM_178290.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein T, 1a (selenot1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178290.6

ORF Insert Sequence:

ATGGGGAAGA TGCGCTGGCT GCCGTTCTCG GCTCTACTAT TATGGGCCTT ATGTCTTCAC 
AGCGCTTCTG CCGATAACAA TGGCGTGAAG AAAATGAAGA TGCAGTTTGC CACGGGTCCA 
CTTCTCAAAT TCCAGATTTG TGTCTCCTGA GGGTACAAGC GGGTGTTTGA GGAGTACACG 
CAGGCTTTGT ACCAGCGGTA CCCAGACATC CGCATAGAGG GAGAGAACTA CCTTCCTCTG 
CCCCTCTACA GGCACATTGC TTCCTTTCTG TCAATGTTTA AGCTGTTGTT AATCGGAGTG 
ATCATCTTGG GCAAGGATCC TTTTGCTTTA TGTGGCATGC AGGCTCCAGG GATCTGGGTT 
TGGAGTCAGG AGAATAAGAT ATATGCTTGT ATGATGGTGT TCTTCTTCAG CAATATGATC 
GAAAATCAGT GCATGTCTAC AGGCGCATTT GAAATCACGC TCAATGATGT CCCAGTTTGG 
TCCAAGCTGG AGTCAGGACA CCTGCCTTCA ATGCAGCAGC TAGTCCAAAT CCTGGAGAAT 
GAGATGAAGA TGAGCATGCA CATGGACACA CTTCCACCCC ATCAGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Scientific reports510673(2015 Jun)
Hsu CH,Lin JS,Po Lai K,Li JW,Chan TF,You MS,Tse WK,Jiang YJ

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

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selenot1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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