selenon cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenon
Entrez Gene ID	352914
Full Name	selenoprotein N
Synonyms	SePN,cb686,selN,sepn1,wu:fb06g01,wu:fb73d02,wu:fv41b08,zgc:101091
General protein information	Preferred Names selenoprotein N Names selenoprotein N selenoprotein N, 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in the orthologous gene in human are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. Inhibition of this gene in zebrafish also showed the importance of this gene in muscle organization during early development. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs in vertebrates contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004294.4	NP_001004294.4	selenoprotein N

Pathways from BioSystems

Homologies

	SEPN1	XP_002802303.1
Danio rerio (zebrafish)	sepn1	NP_001004294.4
Xenopus tropicalis (tropical clawed frog)	sepn1	NP_001070901.1
Rattus norvegicus (Norway rat)	Sepn1	XP_006239194.1
Gallus gallus (chicken)	SEPN1	NP_001108444.1
Bos taurus (cattle)	SEPN1	NP_001108448.1
Mus musculus (house mouse)	Sepn1	NP_083376.2
Homo sapiens (human)	SEPN1	NP_996809.1
Pan troglodytes (chimpanzee)	SEPN1	XP_003307954.2
Canis lupus familiaris (dog)	SEPN1	NP_001108447.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Selenoprotein N is required for ryanodine receptor calcium release channel activity in human and zebrafish muscle.
Jurynec MJ, Xia R, Mackrill JJ, Gunther D, Crawford T, Flanigan KM, Abramson JJ, Howard MT, Grunwald DJ
Proceedings of the National Academy of Sciences of the United States of America105(34)12485-90(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

sepn1 and ryr1 are required for the same cellular differentiation events and are needed for normal calcium fluxes
Title: Selenoprotein N is required for ryanodine receptor calcium release channel activity in human and zebrafish muscle.

alteration of myofibril architecture and tendon-like structure in embryos deficient for SelN function provide new insights into the pathological mechanism of SelN-related myopathy
Title: Loss of selenoprotein N function causes disruption of muscle architecture in the zebrafish embryo.

The following selenon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00601	NM_001004294.4 Latest version!	Danio rerio selenoprotein N (selenon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenon ( NM_001004294.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004294.4 Danio rerio selenoprotein N (selenon), mRNA.

Price & Availability↑

CloneID	ODa00601
Clone ID Related Accession (Same CDS sequence)	NM_001004294.4
Accession Version	NM_001004294.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Danio rerio(zebrafish)
Product	selenoprotein N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EB794202.1, BC078430.1, AY221262.1, BM103116.1, EH479327.1, BM103963.1, CT668851.2 and CT678693.2. On Feb 4, 2010 this sequence version replaced NM_001004294.3. ##Evidence-Data-START## Transcript exon combination :: DQ160295.1, BC078430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ACGTGGATAA AACTCCAGCA GGAGAGCAAA AGGATGATCA TGAGGATCGC 
GGGACTCCAT CCAGCAGACG CGGCCGCTCA CGTTTCACAC AGATCTCCAG TTTATTCATC 
ATTGCTGCTA TTCCTGTCAT CAGCTTTTGC ATCAAATATT ACCTGGACAT CCAGTTTGTG 
AAACGACACG AGGCAGGACT GAAGGCACTG GGAGCAGACG GACTGTTTTT CTTCTCATCC 
TTGGATACTG ATCATGACCT TTATCTCAGT CCAGAGGAAT TCAAACCTAT CGCAGAAAAA 
CTCACAGGTG TGGCCCCTCC CCCAGAGTAT GAAGAGGAGA TTCCACATGA CCCAAATGGA 
GAGACGCTGA CCCTGCATGC TAAAATGCAG CCACTGCTGC TGGAAAGCAT GACGAAGAGC 
AAAGATGGCT TTCTAGGGGT CTCCCACAGC TCTCTTAGTG GCCTGCGCTC TTGGAAACGT 
CCAGCTATTT CTTCATCCAC GTTTTATGCA AGTCAGTTCA AGGTCTTCCT GCCGCCCAGT 
GGTAAATCGG CCGTGGGTGA CACTTGGTGG ATCATTCCCA GTGAACTGAA CATCTTCACT 
GGCTACCTAC CCAACAACCG TTTTCATCCT CCCACACCGC GAGGCAAGGA GGTGCTGATC 
CACTCTCTGC TCAGCATGTT TCACCCACGG CCTTTTGTAA AATCTCGCTT CGCTCCACAG 
GGAGCTGTGG CTTGTATACG GGCTACTAGT GACTTTTATT ATGACATTGT TTTTAGAATC 
CATGCTGAGT TCCAGCTAAA TGATGTTCCA GATTTCCCAT TTTGGTTCAC TCCTGGGCAG 
TTTGCCGGTC ACATTATTCT CTCTAAAGAT GCTTCCCATG TCCGAGATTT TCACATCTAC 
GTCCCAAATG ACAAAACTCT GAATGTGGAC ATGGAGTGGC TGTACGGGGC CAGCGAGACC 
AGTAACATGG AAGTGGATAT TGGATACCTT CCTCAGATGG AGCTTGGTGC TGAAGGACCC 
TCCACTCCCT CAGTGATCTA CGATGAGCAG GGGAACATGA TCGACAGTCG AGGAGAAGGA 
GGTGAACCCA TTCAGTTTGT TTTTGAGGAG ATAGTCTGGA GTGAAGAGCT GAAAAGAGAA 
GAAGCATCCA GACGTCTGGA AGTCACAATG TACCCGTTTA AAAAGGTGCC ATATTTACCA 
TTCAGTGAGG CCTTCAGCAG AGCTTCAGCT GAAAAGAAGC TGGTTCACTC CATCCTGCTC 
TGGGGAGCGC TGGATGACCA GTCATGCTGA GGTTCAGGGC GGACTCTTCG GGAGACAGTT 
CTCGAGAGTT CGCCCGTTCT GGCCTTACTC AACCAGAGCT TCATCAGCAG CTGGTCTCTT 
GTCAAAGAGC TGGAGGATCT GCAGGGTGAC GTGAAAAATC TGGAATTGAG TGAAAAGGCT 
CGTTTACATT TGGAGAAGTA TACTTTTCCA GTGCAGATGA TGGTGGTGCT GCCCAATGGC 
ACTGTAGTCC ATCATATCAA TGCCAATAAT TTCCTGGATC AAACATCTAT GAAACCAGAA 
GATGAAGGGC CAGGTCTTAG TTTCTCAGCA GGTTTTGAGG ATCCCTCAAC TTCCACATAC 
ATCCGCTTCC TGCAGGAAGG ACTTGAAAAG GCCAAACCTT ACTTGGAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004294.4
CDS	183..1856
Translation	MATDVDKTPAGEQKDDHEDRGTPSSRRGRSRFTQISSLFIIAAIPVISFCIKYYLDIQFVKRHEAGLKALGADGLFFFSSLDTDHDLYLSPEEFKPIAEKLTGVAPPPEYEEEIPHDPNGETLTLHAKMQPLLLESMTKSKDGFLGVSHSSLSGLRSWKRPAISSSTFYASQFKVFLPPSGKSAVGDTWWIIPSELNIFTGYLPNNRFHPPTPRGKEVLIHSLLSMFHPRPFVKSRFAPQGAVACIRATSDFYYDIVFRIHAEFQLNDVPDFPFWFTPGQFAGHIILSKDASHVRDFHIYVPNDKTLNVDMEWLYGASETSNMEVDIGYLPQMELGAEGPSTPSVIYDEQGNMIDSRGEGGEPIQFVFEEIVWSEELKREEASRRLEVTMYPFKKVPYLPFSEAFSRASAEKKLVHSILLWGALDDQSC*GSGRTLRETVLESSPVLALLNQSFISSWSLVKELEDLQGDVKNLELSEKARLHLEKYTFPVQMMVVLPNGTVVHHINANNFLDQTSMKPEDEGPGLSFSAGFEDPSTSTYIRFLQEGLEKAKPYLES

Target ORF information:

RefSeq Version	NM_001004294.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein N (selenon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004294.4

ORF Insert Sequence:

ATGGCCACAG ACGTGGATAA AACTCCAGCA GGAGAGCAAA AGGATGATCA TGAGGATCGC 
GGGACTCCAT CCAGCAGACG CGGCCGCTCA CGTTTCACAC AGATCTCCAG TTTATTCATC 
ATTGCTGCTA TTCCTGTCAT CAGCTTTTGC ATCAAATATT ACCTGGACAT CCAGTTTGTG 
AAACGACACG AGGCAGGACT GAAGGCACTG GGAGCAGACG GACTGTTTTT CTTCTCATCC 
TTGGATACTG ATCATGACCT TTATCTCAGT CCAGAGGAAT TCAAACCTAT CGCAGAAAAA 
CTCACAGGTG TGGCCCCTCC CCCAGAGTAT GAAGAGGAGA TTCCACATGA CCCAAATGGA 
GAGACGCTGA CCCTGCATGC TAAAATGCAG CCACTGCTGC TGGAAAGCAT GACGAAGAGC 
AAAGATGGCT TTCTAGGGGT CTCCCACAGC TCTCTTAGTG GCCTGCGCTC TTGGAAACGT 
CCAGCTATTT CTTCATCCAC GTTTTATGCA AGTCAGTTCA AGGTCTTCCT GCCGCCCAGT 
GGTAAATCGG CCGTGGGTGA CACTTGGTGG ATCATTCCCA GTGAACTGAA CATCTTCACT 
GGCTACCTAC CCAACAACCG TTTTCATCCT CCCACACCGC GAGGCAAGGA GGTGCTGATC 
CACTCTCTGC TCAGCATGTT TCACCCACGG CCTTTTGTAA AATCTCGCTT CGCTCCACAG 
GGAGCTGTGG CTTGTATACG GGCTACTAGT GACTTTTATT ATGACATTGT TTTTAGAATC 
CATGCTGAGT TCCAGCTAAA TGATGTTCCA GATTTCCCAT TTTGGTTCAC TCCTGGGCAG 
TTTGCCGGTC ACATTATTCT CTCTAAAGAT GCTTCCCATG TCCGAGATTT TCACATCTAC 
GTCCCAAATG ACAAAACTCT GAATGTGGAC ATGGAGTGGC TGTACGGGGC CAGCGAGACC 
AGTAACATGG AAGTGGATAT TGGATACCTT CCTCAGATGG AGCTTGGTGC TGAAGGACCC 
TCCACTCCCT CAGTGATCTA CGATGAGCAG GGGAACATGA TCGACAGTCG AGGAGAAGGA 
GGTGAACCCA TTCAGTTTGT TTTTGAGGAG ATAGTCTGGA GTGAAGAGCT GAAAAGAGAA 
GAAGCATCCA GACGTCTGGA AGTCACAATG TACCCGTTTA AAAAGGTGCC ATATTTACCA 
TTCAGTGAGG CCTTCAGCAG AGCTTCAGCT GAAAAGAAGC TGGTTCACTC CATCCTGCTC 
TGGGGAGCGC TGGATGACCA GTCATGCTGA GGTTCAGGGC GGACTCTTCG GGAGACAGTT 
CTCGAGAGTT CGCCCGTTCT GGCCTTACTC AACCAGAGCT TCATCAGCAG CTGGTCTCTT 
GTCAAAGAGC TGGAGGATCT GCAGGGTGAC GTGAAAAATC TGGAATTGAG TGAAAAGGCT 
CGTTTACATT TGGAGAAGTA TACTTTTCCA GTGCAGATGA TGGTGGTGCT GCCCAATGGC 
ACTGTAGTCC ATCATATCAA TGCCAATAAT TTCCTGGATC AAACATCTAT GAAACCAGAA 
GATGAAGGGC CAGGTCTTAG TTTCTCAGCA GGTTTTGAGG ATCCCTCAAC TTCCACATAC 
ATCCGCTTCC TGCAGGAAGG ACTTGAAAAG GCCAAACCTT ACTTGGAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Selenoprotein N is required for ryanodine receptor calcium release channel activity in human and zebrafish muscle.
Proceedings of the National Academy of Sciences of the United States of America105(34)12485-90(2008 Aug)
Jurynec MJ,Xia R,Mackrill JJ,Gunther D,Crawford T,Flanigan KM,Abramson JJ,Howard MT,Grunwald DJ

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selenon cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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