med14 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	med14
Entrez Gene ID	336923
Full Name	mediator complex subunit 14
Synonyms	EXLM1,crsp150,crsp2,trap170,wu:fd21c05,wu:fk28h11
General protein information	Preferred Names mediator complex subunit 14 Names mediator of RNA polymerase II transcription subunit 14 cofactor required for Sp1 transcriptional activation, subunit 2 log logelei
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167821.4	XP_005167878.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_005167821.3	XP_005167878.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
NM_212765.2	NP_997930.2	mediator of RNA polymerase II transcription subunit 14
XM_005167822.3	XP_005167879.1	mediator of RNA polymerase II transcription subunit 14 isoform X2

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.
Lou X, Burrows JT, Scott IC
BMC developmental biology1541(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

An in?vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations.
Burrows JT, Pearson BJ, Scott IC
Stem cell reports4(4)670-84(2015 Apr)

A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos.
Jin SW, Herzog W, Santoro MM, Mitchell TS, Frantsve J, Jungblut B, Beis D, Scott IC, D'Amico LA, Ober EA, Verkade H, Field HA, Chi NC, Wehman AM, Baier H, Stainier DY
Developmental biology307(1)29-42(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transplantation experiments demonstrated that med14 and brg1 are required directly in neural crest cells. Analysis of med14; brg1 double mutant embryos suggested the existence of strong genetic interaction between members of Mediator and BAF complexes.
Title: Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.

The following med14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32051	NM_212765.2 Latest version!	Danio rerio mediator complex subunit 14 (med14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa15389	XM_005167821.4 Latest version!	Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa15390	XM_005167822.3 Latest version!	Danio rerio mediator complex subunit 14 (med14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa15389	XM_005167821.3	Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med14 ( NM_212765.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997930.2 Danio rerio mediator complex subunit 14 (med14), mRNA.

Price & Availability↑

CloneID	ODa32051
Clone ID Related Accession (Same CDS sequence)	NM_212765.2
Accession Version	NM_212765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 14
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX294189.11. On Jul 23, 2010 this sequence version replaced NM_212765.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGCAGGGAAC 
ATCCACGCAG CCAATTCCCC TAGTGGAGCT CTGCGGGCCC CTTCTCCCTT TGGGCCCACC 
CCTTCTCCCT CTTCTCTGGG CATTTCTATG GGGCAGACCT CCAACTTTGC CAGCCCACAT 
GGAACTCTTG ACCCAAGTTC ACCATACACA CTAGTGTCCG CCAGCCCTAG GGTAGGCCCC 
TGGCCAGGTT CCCCACAGGT TTCTGGACCT TCTCCTGGAG CACGAATCCC TGGCATGTCA 
CCAGGAAACC CTTCTTTGCA CTCACCCATT CCAGACCCAT CCCACTCCCC TCGAGCTGGT 
ACCAGTTCAC AGGCGATGCC TCCACCTCGT AAACTGCCAC AGCGGTCTTG GGCAGCCTCT 
ATTCCCACCA TCCTCACCCA CAGCAAGCTA CATGTGTTGC TTCTGCCTTC ACCCATGCCA 
TGCCCGGTCC CAGGGCTGGC AGGCAGCTAC CTGTGCTCAC CTCTGGAGCG CTTCCTGGGC 
TCAGTCATCA TGAGACGACA CCTGCAGAGA ATCATCCAAT TAGAACCCAA CCTCACTATT 
GTGAACTCAA ATGAACCAGG CGTCATCATG TTTAAAACAG AAGTGCTTAA ATGCAGAGTG 
GCTCTCAACC CTAAATCCTA CCAGACTCTC CAGCTGAAAG TCACACCAGA AAACACGGGA 
CCCTGGTCAC AGGAGGAGCT GCAGGTCTTG GAGAAGTTCT TTGAGACCAG GGTTGCAGGA 
CCACCCTTTA AGTACAACAC ACTTAATGCC TTTACAAAAC TGTTGGGAGC TCCCACCAAC 
ATACTCAGAG ACTGTGTTCG CATCATGAAA TTGGAGTTGT TCCCAGACCA GGCGGCTCAG 
TTGAACTGGA ATGTGCAGTT CTGTCTGACC ATCCCACCCA GCGCTCCTCC CATTGCCCCA 
CCAGGAACCA TCGCTGTGGT GCTAAAGACC AAGATGCTCT TCTTTCTCCA GCTGACCCAG 
AGGTTACCAG TGCCCCAGGA GCCCATCAGT ATCATCGTGC CTATTGTGTA TGACATGGCC 
ACTGGACTGA CCCAACAGGC CGACATCCCT CGCCAGCACA GCTCTTCAGG AGCCGCGGCC 
CTGATGGTCA GCTCCATCCT CAGACGCTTC AATGAGCACC ATCCACCACG ACCAGGGGAG 
TGCACAATAT TTGCAGCTGT TCACGAGCTC ATGGCTAATC TGACGCTAAA CTCCGGTGGA 
CGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997930.2
CDS	208..4476
Translation	MAPVQIGSDGQLVPAGGPTSTPQPPPGAPATQGVRLSLLIEFLLQRTYHEITLLAELLPRKTDMERKIEIVQFASRTRQLFVRLLALVKWASNAGKVEKCAMISSFLDQQAYLFVDTADRLASLARDALVHARLPSFAIPFAIDVLTTGSYPRLPTCIRDKIIPPDPITKAEKQTTLNQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQVNYIHELVQSRLFGDDKPLQDMYTCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYIPAKCLTLSVWNQQVLGRKTGTASVHKVTIKIDESDGSKPLQISHEPPLPACDSKLMERAMKIDHLSVEKLLIDSVHARSHQKLQELKAILKSSNPNDNSFIETALPTLVIPILEPCGRSECLHIFVDLHSGMFQPMLYGVDQSMLDDIEKSINDDMKRIVTWLLQLKFWLGEQRCRQSVKHLPTLCTDTLHLSNLTTHPVGNLSKHRLFIKLTRLPQYYIVVEMFDVPSNLTELQYKYYFLSVSQMESEDGLPSAQLFRHFKPNLEELVQDISSCRMVRPGTKRKLSGEQSAVEPKKPKRSGEMCAFNKELAHLVAMCDTNMPLIGLRSELSNMEIPHQGVQVEGDGCSHAIRILKVPPCMAVGEETRKALERSLLDCTFRLQGRNNRTWVAELIFSSCPLNSTSIKEQASTRHVYLTYENPLSEPVGGRKVVEMFLNDWNSISKLYECVLEFSRSLTEMPSYLSLFSEIRLYNYRKLVLCYGSTKGSSVTIQWNSVTHRYHLALGTVGPNSGCSNCHNIILHQLQEMFNKTPNVVQLLQVLSDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNTKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPISMEDTFMPHTQTQPPGRGVYPPLTSPPNPYHVPPSPSMITTQSPAGNIHAANSPSGALRAPSPFGPTPSPSSLGISMGQTSNFASPHGTLDPSSPYTLVSASPRVGPWPGSPQVSGPSPGARIPGMSPGNPSLHSPIPDPSHSPRAGTSSQAMPPPRKLPQRSWAASIPTILTHSKLHVLLLPSPMPCPVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQLEPNLTIVNSNEPGVIMFKTEVLKCRVALNPKSYQTLQLKVTPENTGPWSQEELQVLEKFFETRVAGPPFKYNTLNAFTKLLGAPTNILRDCVRIMKLELFPDQAAQLNWNVQFCLTIPPSAPPIAPPGTIAVVLKTKMLFFLQLTQRLPVPQEPISIIVPIVYDMATGLTQQADIPRQHSSSGAAALMVSSILRRFNEHHPPRPGECTIFAAVHELMANLTLNSGGRP

Target ORF information:

RefSeq Version	NM_212765.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 14 (med14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212765.2

ORF Insert Sequence:

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGCAGGGAAC 
ATCCACGCAG CCAATTCCCC TAGTGGAGCT CTGCGGGCCC CTTCTCCCTT TGGGCCCACC 
CCTTCTCCCT CTTCTCTGGG CATTTCTATG GGGCAGACCT CCAACTTTGC CAGCCCACAT 
GGAACTCTTG ACCCAAGTTC ACCATACACA CTAGTGTCCG CCAGCCCTAG GGTAGGCCCC 
TGGCCAGGTT CCCCACAGGT TTCTGGACCT TCTCCTGGAG CACGAATCCC TGGCATGTCA 
CCAGGAAACC CTTCTTTGCA CTCACCCATT CCAGACCCAT CCCACTCCCC TCGAGCTGGT 
ACCAGTTCAC AGGCGATGCC TCCACCTCGT AAACTGCCAC AGCGGTCTTG GGCAGCCTCT 
ATTCCCACCA TCCTCACCCA CAGCAAGCTA CATGTGTTGC TTCTGCCTTC ACCCATGCCA 
TGCCCGGTCC CAGGGCTGGC AGGCAGCTAC CTGTGCTCAC CTCTGGAGCG CTTCCTGGGC 
TCAGTCATCA TGAGACGACA CCTGCAGAGA ATCATCCAAT TAGAACCCAA CCTCACTATT 
GTGAACTCAA ATGAACCAGG CGTCATCATG TTTAAAACAG AAGTGCTTAA ATGCAGAGTG 
GCTCTCAACC CTAAATCCTA CCAGACTCTC CAGCTGAAAG TCACACCAGA AAACACGGGA 
CCCTGGTCAC AGGAGGAGCT GCAGGTCTTG GAGAAGTTCT TTGAGACCAG GGTTGCAGGA 
CCACCCTTTA AGTACAACAC ACTTAATGCC TTTACAAAAC TGTTGGGAGC TCCCACCAAC 
ATACTCAGAG ACTGTGTTCG CATCATGAAA TTGGAGTTGT TCCCAGACCA GGCGGCTCAG 
TTGAACTGGA ATGTGCAGTT CTGTCTGACC ATCCCACCCA GCGCTCCTCC CATTGCCCCA 
CCAGGAACCA TCGCTGTGGT GCTAAAGACC AAGATGCTCT TCTTTCTCCA GCTGACCCAG 
AGGTTACCAG TGCCCCAGGA GCCCATCAGT ATCATCGTGC CTATTGTGTA TGACATGGCC 
ACTGGACTGA CCCAACAGGC CGACATCCCT CGCCAGCACA GCTCTTCAGG AGCCGCGGCC 
CTGATGGTCA GCTCCATCCT CAGACGCTTC AATGAGCACC ATCCACCACG ACCAGGGGAG 
TGCACAATAT TTGCAGCTGT TCACGAGCTC ATGGCTAATC TGACGCTAAA CTCCGGTGGA 
CGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_005167821.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167878.1 Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15389
Clone ID Related Accession (Same CDS sequence)	XM_005167821.4 , XM_005167821.3
Accession Version	XM_005167821.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167821.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGGAACATC 
CACGCAGCCA ATTCCCCTAG TGGAGCTCTG CGGGCCCCTT CTCCCTTTGG GCCCACCCCT 
TCTCCCTCTT CTCTGGGCAT TTCTATGGGG CAGACCTCCA ACTTTGCCAG CCCACATGGA 
ACTCTTGACC CAAGTTCACC ATACACACTA GTGTCCGCCA GCCCTAGGGT AGGCCCCTGG 
CCAGGTTCCC CACAGGTTTC TGGACCTTCT CCTGGAGCAC GAATCCCTGG CATGTCACCA 
GGAAACCCTT CTTTGCACTC ACCCATTCCA GACCCATCCC ACTCCCCTCG AGCTGGTACC 
AGTTCACAGG CGATGCCTCC ACCTCGTAAA CTGCCACAGC GGTCTTGGGC AGCCTCTATT 
CCCACCATCC TCACCCACAG CAAGCTACAT GTGTTGCTTC TGCCTTCACC CATGCCATGC 
CCGGTCCCAG GGCTGGCAGG CAGCTACCTG TGCTCACCTC TGGAGCGCTT CCTGGGCTCA 
GTCATCATGA GACGACACCT GCAGAGAATC ATCCAATTAG AACCCAACCT CACTATTGTG 
AACTCAAATG AACCAGGCGT CATCATGTTT AAAACAGAAG TGCTTAAATG CAGAGTGGCT 
CTCAACCCTA AATCCTACCA GACTCTCCAG CTGAAAGTCA CACCAGAAAA CACGGGACCC 
TGGTCACAGG AGGAGCTGCA GGTCTTGGAG AAGTTCTTTG AGACCAGGGT TGCAGGACCA 
CCCTTTAAGT ACAACACACT TAATGCCTTT ACAAAACTGT TGGGAGCTCC CACCAACATA 
CTCAGAGACT GTGTTCGCAT CATGAAATTG GAGTTGTTCC CAGACCAGGC GGCTCAGTTG 
AACTGGAATG TGCAGTTCTG TCTGACCATC CCACCCAGCG CTCCTCCCAT TGCCCCACCA 
GGAACCATCG CTGTGGTGCT AAAGACCAAG ATGCTCTTCT TTCTCCAGCT GACCCAGAGG 
TTACCAGTGC CCCAGGAGCC CATCAGTATC ATCGTGCCTA TTGTGTATGA CATGGCCACT 
GGACTGACCC AACAGGCCGA CATCCCTCGC CAGCACAGCT CTTCAGGAGC CGCGGCCCTG 
ATGGTCAGCT CCATCCTCAG ACGCTTCAAT GAGCACCATC CACCACGACC AGGGGAGTGC 
ACAATATTTG CAGCTGTTCA CGAGCTCATG GCTAATCTGA CGCTAAACTC CGGTGGACGC 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167878.1
CDS	238..4503
Translation	MAPVQIGSDGQLVPAGGPTSTPQPPPGAPATQGVRLSLLIEFLLQRTYHEITLLAELLPRKTDMERKIEIVQFASRTRQLFVRLLALVKWASNAGKVEKCAMISSFLDQQAYLFVDTADRLASLARDALVHARLPSFAIPFAIDVLTTGSYPRLPTCIRDKIIPPDPITKAEKQTTLNQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQVNYIHELVQSRLFGDDKPLQDMYTCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYIPAKCLTLSVWNQQVLGRKTGTASVHKVTIKIDESDGSKPLQISHEPPLPACDSKLMERAMKIDHLSVEKLLIDSVHARSHQKLQELKAILKSSNPNDNSFIETALPTLVIPILEPCGRSECLHIFVDLHSGMFQPMLYGVDQSMLDDIEKSINDDMKRIVTWLLQLKFWLGEQRCRQSVKHLPTLCTDTLHLSNLTTHPVGNLSKHRLFIKLTRLPQYYIVVEMFDVPSNLTELQYKYYFLSVSQMESEDGLPSAQLFRHFKPNLEELVQDISSCRMVRPGTKRKLSGEQSAVEPKKPKRSGEMCAFNKELAHLVAMCDTNMPLIGLRSELSNMEIPHQGVQVEGDGCSHAIRILKVPPCMAVGEETRKALERSLLDCTFRLQGRNNRTWVAELIFSSCPLNSTSIKEQASTRHVYLTYENPLSEPVGGRKVVEMFLNDWNSISKLYECVLEFSRSLTEMPSYLSLFSEIRLYNYRKLVLCYGSTKGSSVTIQWNSVTHRYHLALGTVGPNSGCSNCHNIILHQLQEMFNKTPNVVQLLQVLSDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNTKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPISMEDTFMPHTQTQPPGRGVYPPLTSPPNPYHVPPSPSMITTQSPGNIHAANSPSGALRAPSPFGPTPSPSSLGISMGQTSNFASPHGTLDPSSPYTLVSASPRVGPWPGSPQVSGPSPGARIPGMSPGNPSLHSPIPDPSHSPRAGTSSQAMPPPRKLPQRSWAASIPTILTHSKLHVLLLPSPMPCPVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQLEPNLTIVNSNEPGVIMFKTEVLKCRVALNPKSYQTLQLKVTPENTGPWSQEELQVLEKFFETRVAGPPFKYNTLNAFTKLLGAPTNILRDCVRIMKLELFPDQAAQLNWNVQFCLTIPPSAPPIAPPGTIAVVLKTKMLFFLQLTQRLPVPQEPISIIVPIVYDMATGLTQQADIPRQHSSSGAAALMVSSILRRFNEHHPPRPGECTIFAAVHELMANLTLNSGGRP

Target ORF information:

RefSeq Version	XM_005167821.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167821.4

ORF Insert Sequence:

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGGAACATC 
CACGCAGCCA ATTCCCCTAG TGGAGCTCTG CGGGCCCCTT CTCCCTTTGG GCCCACCCCT 
TCTCCCTCTT CTCTGGGCAT TTCTATGGGG CAGACCTCCA ACTTTGCCAG CCCACATGGA 
ACTCTTGACC CAAGTTCACC ATACACACTA GTGTCCGCCA GCCCTAGGGT AGGCCCCTGG 
CCAGGTTCCC CACAGGTTTC TGGACCTTCT CCTGGAGCAC GAATCCCTGG CATGTCACCA 
GGAAACCCTT CTTTGCACTC ACCCATTCCA GACCCATCCC ACTCCCCTCG AGCTGGTACC 
AGTTCACAGG CGATGCCTCC ACCTCGTAAA CTGCCACAGC GGTCTTGGGC AGCCTCTATT 
CCCACCATCC TCACCCACAG CAAGCTACAT GTGTTGCTTC TGCCTTCACC CATGCCATGC 
CCGGTCCCAG GGCTGGCAGG CAGCTACCTG TGCTCACCTC TGGAGCGCTT CCTGGGCTCA 
GTCATCATGA GACGACACCT GCAGAGAATC ATCCAATTAG AACCCAACCT CACTATTGTG 
AACTCAAATG AACCAGGCGT CATCATGTTT AAAACAGAAG TGCTTAAATG CAGAGTGGCT 
CTCAACCCTA AATCCTACCA GACTCTCCAG CTGAAAGTCA CACCAGAAAA CACGGGACCC 
TGGTCACAGG AGGAGCTGCA GGTCTTGGAG AAGTTCTTTG AGACCAGGGT TGCAGGACCA 
CCCTTTAAGT ACAACACACT TAATGCCTTT ACAAAACTGT TGGGAGCTCC CACCAACATA 
CTCAGAGACT GTGTTCGCAT CATGAAATTG GAGTTGTTCC CAGACCAGGC GGCTCAGTTG 
AACTGGAATG TGCAGTTCTG TCTGACCATC CCACCCAGCG CTCCTCCCAT TGCCCCACCA 
GGAACCATCG CTGTGGTGCT AAAGACCAAG ATGCTCTTCT TTCTCCAGCT GACCCAGAGG 
TTACCAGTGC CCCAGGAGCC CATCAGTATC ATCGTGCCTA TTGTGTATGA CATGGCCACT 
GGACTGACCC AACAGGCCGA CATCCCTCGC CAGCACAGCT CTTCAGGAGC CGCGGCCCTG 
ATGGTCAGCT CCATCCTCAG ACGCTTCAAT GAGCACCATC CACCACGACC AGGGGAGTGC 
ACAATATTTG CAGCTGTTCA CGAGCTCATG GCTAATCTGA CGCTAAACTC CGGTGGACGC 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_005167821.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167878.1 Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.

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CloneID	ODa15389
Clone ID Related Accession (Same CDS sequence)	XM_005167821.4 , XM_005167821.3
Accession Version	XM_005167821.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGGAACATC 
CACGCAGCCA ATTCCCCTAG TGGAGCTCTG CGGGCCCCTT CTCCCTTTGG GCCCACCCCT 
TCTCCCTCTT CTCTGGGCAT TTCTATGGGG CAGACCTCCA ACTTTGCCAG CCCACATGGA 
ACTCTTGACC CAAGTTCACC ATACACACTA GTGTCCGCCA GCCCTAGGGT AGGCCCCTGG 
CCAGGTTCCC CACAGGTTTC TGGACCTTCT CCTGGAGCAC GAATCCCTGG CATGTCACCA 
GGAAACCCTT CTTTGCACTC ACCCATTCCA GACCCATCCC ACTCCCCTCG AGCTGGTACC 
AGTTCACAGG CGATGCCTCC ACCTCGTAAA CTGCCACAGC GGTCTTGGGC AGCCTCTATT 
CCCACCATCC TCACCCACAG CAAGCTACAT GTGTTGCTTC TGCCTTCACC CATGCCATGC 
CCGGTCCCAG GGCTGGCAGG CAGCTACCTG TGCTCACCTC TGGAGCGCTT CCTGGGCTCA 
GTCATCATGA GACGACACCT GCAGAGAATC ATCCAATTAG AACCCAACCT CACTATTGTG 
AACTCAAATG AACCAGGCGT CATCATGTTT AAAACAGAAG TGCTTAAATG CAGAGTGGCT 
CTCAACCCTA AATCCTACCA GACTCTCCAG CTGAAAGTCA CACCAGAAAA CACGGGACCC 
TGGTCACAGG AGGAGCTGCA GGTCTTGGAG AAGTTCTTTG AGACCAGGGT TGCAGGACCA 
CCCTTTAAGT ACAACACACT TAATGCCTTT ACAAAACTGT TGGGAGCTCC CACCAACATA 
CTCAGAGACT GTGTTCGCAT CATGAAATTG GAGTTGTTCC CAGACCAGGC GGCTCAGTTG 
AACTGGAATG TGCAGTTCTG TCTGACCATC CCACCCAGCG CTCCTCCCAT TGCCCCACCA 
GGAACCATCG CTGTGGTGCT AAAGACCAAG ATGCTCTTCT TTCTCCAGCT GACCCAGAGG 
TTACCAGTGC CCCAGGAGCC CATCAGTATC ATCGTGCCTA TTGTGTATGA CATGGCCACT 
GGACTGACCC AACAGGCCGA CATCCCTCGC CAGCACAGCT CTTCAGGAGC CGCGGCCCTG 
ATGGTCAGCT CCATCCTCAG ACGCTTCAAT GAGCACCATC CACCACGACC AGGGGAGTGC 
ACAATATTTG CAGCTGTTCA CGAGCTCATG GCTAATCTGA CGCTAAACTC CGGTGGACGC 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167878.1
CDS	239..4504
Translation	MAPVQIGSDGQLVPAGGPTSTPQPPPGAPATQGVRLSLLIEFLLQRTYHEITLLAELLPRKTDMERKIEIVQFASRTRQLFVRLLALVKWASNAGKVEKCAMISSFLDQQAYLFVDTADRLASLARDALVHARLPSFAIPFAIDVLTTGSYPRLPTCIRDKIIPPDPITKAEKQTTLNQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQVNYIHELVQSRLFGDDKPLQDMYTCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYIPAKCLTLSVWNQQVLGRKTGTASVHKVTIKIDESDGSKPLQISHEPPLPACDSKLMERAMKIDHLSVEKLLIDSVHARSHQKLQELKAILKSSNPNDNSFIETALPTLVIPILEPCGRSECLHIFVDLHSGMFQPMLYGVDQSMLDDIEKSINDDMKRIVTWLLQLKFWLGEQRCRQSVKHLPTLCTDTLHLSNLTTHPVGNLSKHRLFIKLTRLPQYYIVVEMFDVPSNLTELQYKYYFLSVSQMESEDGLPSAQLFRHFKPNLEELVQDISSCRMVRPGTKRKLSGEQSAVEPKKPKRSGEMCAFNKELAHLVAMCDTNMPLIGLRSELSNMEIPHQGVQVEGDGCSHAIRILKVPPCMAVGEETRKALERSLLDCTFRLQGRNNRTWVAELIFSSCPLNSTSIKEQASTRHVYLTYENPLSEPVGGRKVVEMFLNDWNSISKLYECVLEFSRSLTEMPSYLSLFSEIRLYNYRKLVLCYGSTKGSSVTIQWNSVTHRYHLALGTVGPNSGCSNCHNIILHQLQEMFNKTPNVVQLLQVLSDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNTKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPISMEDTFMPHTQTQPPGRGVYPPLTSPPNPYHVPPSPSMITTQSPGNIHAANSPSGALRAPSPFGPTPSPSSLGISMGQTSNFASPHGTLDPSSPYTLVSASPRVGPWPGSPQVSGPSPGARIPGMSPGNPSLHSPIPDPSHSPRAGTSSQAMPPPRKLPQRSWAASIPTILTHSKLHVLLLPSPMPCPVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQLEPNLTIVNSNEPGVIMFKTEVLKCRVALNPKSYQTLQLKVTPENTGPWSQEELQVLEKFFETRVAGPPFKYNTLNAFTKLLGAPTNILRDCVRIMKLELFPDQAAQLNWNVQFCLTIPPSAPPIAPPGTIAVVLKTKMLFFLQLTQRLPVPQEPISIIVPIVYDMATGLTQQADIPRQHSSSGAAALMVSSILRRFNEHHPPRPGECTIFAAVHELMANLTLNSGGRP

Target ORF information:

RefSeq Version	XM_005167821.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167821.3

ORF Insert Sequence:

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGGAACATC 
CACGCAGCCA ATTCCCCTAG TGGAGCTCTG CGGGCCCCTT CTCCCTTTGG GCCCACCCCT 
TCTCCCTCTT CTCTGGGCAT TTCTATGGGG CAGACCTCCA ACTTTGCCAG CCCACATGGA 
ACTCTTGACC CAAGTTCACC ATACACACTA GTGTCCGCCA GCCCTAGGGT AGGCCCCTGG 
CCAGGTTCCC CACAGGTTTC TGGACCTTCT CCTGGAGCAC GAATCCCTGG CATGTCACCA 
GGAAACCCTT CTTTGCACTC ACCCATTCCA GACCCATCCC ACTCCCCTCG AGCTGGTACC 
AGTTCACAGG CGATGCCTCC ACCTCGTAAA CTGCCACAGC GGTCTTGGGC AGCCTCTATT 
CCCACCATCC TCACCCACAG CAAGCTACAT GTGTTGCTTC TGCCTTCACC CATGCCATGC 
CCGGTCCCAG GGCTGGCAGG CAGCTACCTG TGCTCACCTC TGGAGCGCTT CCTGGGCTCA 
GTCATCATGA GACGACACCT GCAGAGAATC ATCCAATTAG AACCCAACCT CACTATTGTG 
AACTCAAATG AACCAGGCGT CATCATGTTT AAAACAGAAG TGCTTAAATG CAGAGTGGCT 
CTCAACCCTA AATCCTACCA GACTCTCCAG CTGAAAGTCA CACCAGAAAA CACGGGACCC 
TGGTCACAGG AGGAGCTGCA GGTCTTGGAG AAGTTCTTTG AGACCAGGGT TGCAGGACCA 
CCCTTTAAGT ACAACACACT TAATGCCTTT ACAAAACTGT TGGGAGCTCC CACCAACATA 
CTCAGAGACT GTGTTCGCAT CATGAAATTG GAGTTGTTCC CAGACCAGGC GGCTCAGTTG 
AACTGGAATG TGCAGTTCTG TCTGACCATC CCACCCAGCG CTCCTCCCAT TGCCCCACCA 
GGAACCATCG CTGTGGTGCT AAAGACCAAG ATGCTCTTCT TTCTCCAGCT GACCCAGAGG 
TTACCAGTGC CCCAGGAGCC CATCAGTATC ATCGTGCCTA TTGTGTATGA CATGGCCACT 
GGACTGACCC AACAGGCCGA CATCCCTCGC CAGCACAGCT CTTCAGGAGC CGCGGCCCTG 
ATGGTCAGCT CCATCCTCAG ACGCTTCAAT GAGCACCATC CACCACGACC AGGGGAGTGC 
ACAATATTTG CAGCTGTTCA CGAGCTCATG GCTAATCTGA CGCTAAACTC CGGTGGACGC 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_005167822.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167879.1 Danio rerio mediator complex subunit 14 (med14), transcript variant X2, mRNA.

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CloneID	ODa15390
Clone ID Related Accession (Same CDS sequence)	XM_005167822.3
Accession Version	XM_005167822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGAACTCTT 
GACCCAAGTT CACCATACAC ACTAGTGTCC GCCAGCCCTA GGGTAGGCCC CTGGCCAGGT 
TCCCCACAGG TTTCTGGACC TTCTCCTGGA GCACGAATCC CTGGCATGTC ACCAGGAAAC 
CCTTCTTTGC ACTCACCCAT TCCAGACCCA TCCCACTCCC CTCGAGCTGG TACCAGTTCA 
CAGGCGATGC CTCCACCTCG TAAACTGCCA CAGCGGTCTT GGGCAGCCTC TATTCCCACC 
ATCCTCACCC ACAGCAAGCT ACATGTGTTG CTTCTGCCTT CACCCATGCC ATGCCCGGTC 
CCAGGGCTGG CAGGCAGCTA CCTGTGCTCA CCTCTGGAGC GCTTCCTGGG CTCAGTCATC 
ATGAGACGAC ACCTGCAGAG AATCATCCAA TTAGAACCCA ACCTCACTAT TGTGAACTCA 
AATGAACCAG GCGTCATCAT GTTTAAAACA GAAGTGCTTA AATGCAGAGT GGCTCTCAAC 
CCTAAATCCT ACCAGACTCT CCAGCTGAAA GTCACACCAG AAAACACGGG ACCCTGGTCA 
CAGGAGGAGC TGCAGGTCTT GGAGAAGTTC TTTGAGACCA GGGTTGCAGG ACCACCCTTT 
AAGTACAACA CACTTAATGC CTTTACAAAA CTGTTGGGAG CTCCCACCAA CATACTCAGA 
GACTGTGTTC GCATCATGAA ATTGGAGTTG TTCCCAGACC AGGCGGCTCA GTTGAACTGG 
AATGTGCAGT TCTGTCTGAC CATCCCACCC AGCGCTCCTC CCATTGCCCC ACCAGGAACC 
ATCGCTGTGG TGCTAAAGAC CAAGATGCTC TTCTTTCTCC AGCTGACCCA GAGGTTACCA 
GTGCCCCAGG AGCCCATCAG TATCATCGTG CCTATTGTGT ATGACATGGC CACTGGACTG 
ACCCAACAGG CCGACATCCC TCGCCAGCAC AGCTCTTCAG GAGCCGCGGC CCTGATGGTC 
AGCTCCATCC TCAGACGCTT CAATGAGCAC CATCCACCAC GACCAGGGGA GTGCACAATA 
TTTGCAGCTG TTCACGAGCT CATGGCTAAT CTGACGCTAA ACTCCGGTGG ACGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167879.1
CDS	208..4347
Translation	MAPVQIGSDGQLVPAGGPTSTPQPPPGAPATQGVRLSLLIEFLLQRTYHEITLLAELLPRKTDMERKIEIVQFASRTRQLFVRLLALVKWASNAGKVEKCAMISSFLDQQAYLFVDTADRLASLARDALVHARLPSFAIPFAIDVLTTGSYPRLPTCIRDKIIPPDPITKAEKQTTLNQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQVNYIHELVQSRLFGDDKPLQDMYTCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYIPAKCLTLSVWNQQVLGRKTGTASVHKVTIKIDESDGSKPLQISHEPPLPACDSKLMERAMKIDHLSVEKLLIDSVHARSHQKLQELKAILKSSNPNDNSFIETALPTLVIPILEPCGRSECLHIFVDLHSGMFQPMLYGVDQSMLDDIEKSINDDMKRIVTWLLQLKFWLGEQRCRQSVKHLPTLCTDTLHLSNLTTHPVGNLSKHRLFIKLTRLPQYYIVVEMFDVPSNLTELQYKYYFLSVSQMESEDGLPSAQLFRHFKPNLEELVQDISSCRMVRPGTKRKLSGEQSAVEPKKPKRSGEMCAFNKELAHLVAMCDTNMPLIGLRSELSNMEIPHQGVQVEGDGCSHAIRILKVPPCMAVGEETRKALERSLLDCTFRLQGRNNRTWVAELIFSSCPLNSTSIKEQASTRHVYLTYENPLSEPVGGRKVVEMFLNDWNSISKLYECVLEFSRSLTEMPSYLSLFSEIRLYNYRKLVLCYGSTKGSSVTIQWNSVTHRYHLALGTVGPNSGCSNCHNIILHQLQEMFNKTPNVVQLLQVLSDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNTKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPISMEDTFMPHTQTQPPGRGVYPPLTSPPNPYHVPPSPSMITTQSPGTLDPSSPYTLVSASPRVGPWPGSPQVSGPSPGARIPGMSPGNPSLHSPIPDPSHSPRAGTSSQAMPPPRKLPQRSWAASIPTILTHSKLHVLLLPSPMPCPVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQLEPNLTIVNSNEPGVIMFKTEVLKCRVALNPKSYQTLQLKVTPENTGPWSQEELQVLEKFFETRVAGPPFKYNTLNAFTKLLGAPTNILRDCVRIMKLELFPDQAAQLNWNVQFCLTIPPSAPPIAPPGTIAVVLKTKMLFFLQLTQRLPVPQEPISIIVPIVYDMATGLTQQADIPRQHSSSGAAALMVSSILRRFNEHHPPRPGECTIFAAVHELMANLTLNSGGRP

Target ORF information:

RefSeq Version	XM_005167822.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 14 (med14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167822.3

ORF Insert Sequence:

ATGGCTCCAG TTCAGATCGG TTCGGACGGG CAGCTGGTGC CCGCCGGGGG TCCGACCTCA 
ACCCCGCAGC CTCCTCCAGG AGCCCCGGCA ACCCAAGGAG TACGGCTGAG CCTTCTGATC 
GAGTTTTTGC TACAGCGAAC CTATCATGAG ATCACACTGC TGGCTGAACT ACTTCCTCGT 
AAAACAGACA TGGAAAGAAA AATTGAAATT GTTCAGTTTG CCAGTCGGAC ACGTCAACTC 
TTTGTGCGTC TGCTTGCTCT TGTCAAATGG GCAAGTAATG CAGGAAAGGT GGAGAAATGT 
GCGATGATCT CCTCATTTCT GGATCAACAA GCATATTTAT TTGTTGACAC GGCTGACCGG 
CTGGCATCAC TAGCCAGGGA TGCGTTGGTA CATGCACGTT TGCCTAGTTT TGCCATCCCT 
TTTGCCATTG ATGTACTAAC CACAGGGTCT TATCCACGTC TGCCCACGTG CATCCGAGAT 
AAAATCATTC CTCCCGACCC AATAACCAAA GCAGAGAAGC AGACCACGCT AAATCAGCTC 
AATCAAATCC TGCGACACAG ACTAGTCACC ACTGATTTGC CTCCACAGCT GGCTAACTTA 
ACCGTGGCCA ATGGACGAGT CAAGTTTCGT GTTGAGGGTG AATTTGAAGC AACGCTGACG 
GTGATGGGGG ATGATCCTGA TATTCCCTGG AGACTTCTTA AACTAGAGAT TCTGGTTGAG 
GATAAGGAAA CTGGAGATGG ACGGGCATTG GTCCATAGCA TGCAGGTGAA TTATATCCAC 
GAGCTGGTCC AGTCCCGCTT GTTTGGAGAT GACAAGCCTC TTCAGGACAT GTACACCTGC 
CTCCATTCAT TCTGCCTGTC ACTCCAACTG GAGGTTCTGC ACTCTCAGAC TCTAATGTTA 
ATCAGAGAGC GGTGGGGGGA TCTGGTGCAG GTCGAGCGCT ACATCCCTGC AAAGTGCCTC 
ACATTATCTG TCTGGAATCA ACAGGTGCTG GGTAGAAAGA CTGGCACGGC ATCTGTTCAC 
AAGGTTACCA TAAAAATCGA TGAAAGCGAT GGTTCTAAGC CATTACAGAT CTCCCATGAG 
CCTCCACTGC CTGCCTGTGA TTCCAAATTA ATGGAAAGAG CCATGAAGAT CGACCATCTC 
TCAGTGGAAA AGCTGTTGAT TGATAGTGTA CATGCTCGCT CACATCAGAA ACTGCAAGAA 
CTTAAAGCCA TTCTGAAAAG TAGCAACCCT AATGACAACT CCTTTATAGA GACTGCTCTG 
CCCACGCTGG TCATACCAAT ACTGGAGCCC TGCGGTCGAT CTGAGTGCCT CCACATATTT 
GTCGATCTGC ATTCAGGAAT GTTTCAGCCA ATGCTCTACG GAGTCGATCA ATCCATGCTG 
GACGATATTG AGAAGAGTAT CAACGATGAC ATGAAGCGCA TCGTCACATG GTTACTACAG 
CTAAAATTCT GGTTGGGCGA GCAGAGATGT CGGCAATCCG TGAAACATCT TCCCACACTG 
TGCACAGACA CTCTCCATCT ATCGAACCTA ACCACACATC CTGTGGGCAA CCTTTCAAAA 
CATCGCCTCT TCATCAAACT CACTCGGCTC CCACAATACT ATATTGTGGT GGAGATGTTT 
GACGTGCCCA GCAATCTGAC AGAGCTTCAG TATAAGTACT ACTTCCTGTC TGTGAGTCAG 
ATGGAGAGCG AGGACGGACT TCCTTCTGCT CAGCTGTTTA GGCACTTTAA GCCCAACCTG 
GAGGAGCTTG TGCAGGACAT TTCATCTTGT CGAATGGTTC GCCCTGGGAC CAAGAGGAAG 
TTGTCAGGAG AGCAAAGTGC CGTTGAACCA AAGAAACCCA AGAGGTCTGG AGAGATGTGT 
GCCTTTAATA AGGAGCTTGC TCACCTGGTG GCCATGTGTG ACACCAACAT GCCCCTTATC 
GGACTGCGCT CTGAGTTGTC CAATATGGAG ATTCCGCATC AGGGAGTGCA GGTCGAAGGA 
GACGGCTGTA GTCATGCCAT ACGTATTCTG AAAGTTCCAC CTTGTATGGC AGTTGGTGAA 
GAAACCAGAA AAGCTCTAGA GCGCTCTCTG CTGGACTGCA CCTTCAGACT ACAGGGACGC 
AATAATCGCA CATGGGTGGC AGAATTGATT TTCTCCAGTT GCCCACTCAA CAGCACATCC 
ATCAAAGAGC AAGCTTCAAC GCGGCATGTC TACCTGACAT ATGAGAATCC TCTGTCAGAG 
CCAGTGGGTG GCAGGAAAGT GGTGGAGATG TTTTTGAATG ACTGGAACAG CATTAGTAAG 
CTTTATGAAT GTGTGCTGGA GTTCTCGCGC TCTTTAACTG AAATGCCCTC CTATCTGAGC 
CTTTTCTCGG AGATACGGTT GTACAATTAT CGGAAACTGG TTCTGTGTTA TGGCAGCACT 
AAAGGCAGCT CGGTAACGAT ACAATGGAAC TCAGTGACAC ACCGATACCA TCTGGCCCTG 
GGGACTGTGG GGCCCAATTC AGGCTGTAGC AACTGTCACA ACATAATTCT GCACCAACTT 
CAGGAGATGT TCAACAAGAC ACCCAATGTT GTACAACTTC TGCAGGTCCT CTCAGATACT 
CAGGCTCCTC TAAATGCCAT CAACAAGCTT CCCACAGTGC CTATGTTGGG TCTTACCCAA 
AGGACTAATA CGGCGTACCA GTGCTTTTCC ATCCTGCCCC AGTCGCCCAC CCACATCCGC 
CTGGCCTTCC GCAACATGTA CTGCATTGAC ATCTACTGCC GAAGCAGAGG GGTGGTGGCC 
ATCCGGGATG GGGCCTACAG TTTGTTTGAC AACACTAAGA TAGTCGAAGG CTTCTATCCA 
GCTCCTGGAC TTAAGACGTT CCTGAACATG TTTGTGGATA GTAACCAGGA TGCTCGAAGA 
CGCTCCGTCA ATGAAGACGA CAACCCTCCC TCGCCAATTA GCATGGAGGA CACCTTCATG 
CCCCACACCC AGACACAACC TCCAGGAAGA GGGGTGTATC CGCCCCTTAC ATCACCTCCC 
AACCCGTACC ATGTGCCTCC TTCACCGTCC ATGATCACAA CGCAATCTCC AGGAACTCTT 
GACCCAAGTT CACCATACAC ACTAGTGTCC GCCAGCCCTA GGGTAGGCCC CTGGCCAGGT 
TCCCCACAGG TTTCTGGACC TTCTCCTGGA GCACGAATCC CTGGCATGTC ACCAGGAAAC 
CCTTCTTTGC ACTCACCCAT TCCAGACCCA TCCCACTCCC CTCGAGCTGG TACCAGTTCA 
CAGGCGATGC CTCCACCTCG TAAACTGCCA CAGCGGTCTT GGGCAGCCTC TATTCCCACC 
ATCCTCACCC ACAGCAAGCT ACATGTGTTG CTTCTGCCTT CACCCATGCC ATGCCCGGTC 
CCAGGGCTGG CAGGCAGCTA CCTGTGCTCA CCTCTGGAGC GCTTCCTGGG CTCAGTCATC 
ATGAGACGAC ACCTGCAGAG AATCATCCAA TTAGAACCCA ACCTCACTAT TGTGAACTCA 
AATGAACCAG GCGTCATCAT GTTTAAAACA GAAGTGCTTA AATGCAGAGT GGCTCTCAAC 
CCTAAATCCT ACCAGACTCT CCAGCTGAAA GTCACACCAG AAAACACGGG ACCCTGGTCA 
CAGGAGGAGC TGCAGGTCTT GGAGAAGTTC TTTGAGACCA GGGTTGCAGG ACCACCCTTT 
AAGTACAACA CACTTAATGC CTTTACAAAA CTGTTGGGAG CTCCCACCAA CATACTCAGA 
GACTGTGTTC GCATCATGAA ATTGGAGTTG TTCCCAGACC AGGCGGCTCA GTTGAACTGG 
AATGTGCAGT TCTGTCTGAC CATCCCACCC AGCGCTCCTC CCATTGCCCC ACCAGGAACC 
ATCGCTGTGG TGCTAAAGAC CAAGATGCTC TTCTTTCTCC AGCTGACCCA GAGGTTACCA 
GTGCCCCAGG AGCCCATCAG TATCATCGTG CCTATTGTGT ATGACATGGC CACTGGACTG 
ACCCAACAGG CCGACATCCC TCGCCAGCAC AGCTCTTCAG GAGCCGCGGC CCTGATGGTC 
AGCTCCATCC TCAGACGCTT CAATGAGCAC CATCCACCAC GACCAGGGGA GTGCACAATA 
TTTGCAGCTG TTCACGAGCT CATGGCTAAT CTGACGCTAA ACTCCGGTGG ACGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.
BMC developmental biology1541(2015 Nov)
Lou X,Burrows JT,Scott IC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

An in?vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations.
Stem cell reports4(4)670-84(2015 Apr)
Burrows JT,Pearson BJ,Scott IC

A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos.
Developmental biology307(1)29-42(2007 Jul)
Jin SW,Herzog W,Santoro MM,Mitchell TS,Frantsve J,Jungblut B,Beis D,Scott IC,D'Amico LA,Ober EA,Verkade H,Field HA,Chi NC,Wehman AM,Baier H,Stainier DY

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med14 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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