DHX16 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX16
Entrez Gene ID	336645
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 16
Synonyms	fa91b12,wu:fa91b12,zgc:55590
General protein information	Preferred Names DEAH (Asp-Glu-Ala-His) box polypeptide 16 Names putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 mis mission impossible
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200024.1	NP_956318.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
XM_017359220.1	XP_017214709.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX16	NP_003578.2
Rattus norvegicus (Norway rat)	Dhx16	NP_997661.1
Caenorhabditis elegans (roundworm)	mog-4	NP_497027.1
Schizosaccharomyces pombe (fission yeast)	cdc28	NP_595686.2
Neurospora crassa	NCU08928	XP_960795.1
Drosophila melanogaster (fruit fly)	l(2)37Cb	NP_609946.1
Pan troglodytes (chimpanzee)	DHX16	NP_001035839.1
	DHX16	NP_001098636.1
Mus musculus (house mouse)	Dhx16	NP_081263.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009093	XP_319843.4
Arabidopsis thaliana (thale cress)	ESP3	NP_174527.2
Canis lupus familiaris (dog)	DHX16	XP_538827.2
Danio rerio (zebrafish)	dhx16	NP_956318.1
Bos taurus (cattle)	DHX16	NP_001193139.1
Magnaporthe oryzae (rice blast fungus)	MGG_07501	XP_003711413.1
Arabidopsis thaliana (thale cress)	MEE29	NP_181077.3
Oryza sativa (rice)	Os05g0389800	NP_001055437.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.
Putiri E, Pelegri F
Developmental biology353(2)275-89(2011 May)

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
Sambrook JG, Figueroa F, Beck S
BMC genomics6152(2005 Nov)

Identification of recessive maternal-effect mutations in the zebrafish using a gynogenesis-based method.
Pelegri F, Dekens MP, Schulte-Merker S, Maischein HM, Weiler C, N?sslein-Volhard C
Developmental dynamics : an official publication of the American Association of Anatomists231(2)324-35(2004 Oct)

Identification of 315 genes essential for early zebrafish development.
Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Within the endoderm induction pathway, mis is not required for the activation of early zygotic genes, but is essential to implement nodal activity downstream of casanova/sox 32 but upstream of sox17 expression
Title: The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.

The following DHX16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa55066	XM_017359220.1 Latest version!	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa36296	NM_200024.1 Latest version!	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX16 ( XM_017359220.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214709.1 Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55066
Clone ID Related Accession (Same CDS sequence)	XM_017359220.1
Accession Version	XM_017359220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATC TGGAACAGTG GGTTAGTGAC CAGCTCCATG ACATCCTGGG TTTAAGTGAC 
CGATATGTGG CCCAGTTCAT GATCGGACTG GTTCAGAAGT CGTCTGGACC TCAGGATTTT 
GTGTCTCGGC TTCAGGACAC TGGAACTATT GACATTGATC AAAGAATCAC AGCGTTTGCA 
CATGAGTTGT ACGACAAGGC TCCCAAAAAG CATGTGGTTG AAAAACCTGC TCGAGCAATC 
GAACGTCAGG TCATGGAGAT GGAGAGAAAG AACAGAACCT ACACCCTTCT GGAGGACAGC 
GAGAGCGATG GAGAGGCAGC CAGAGAAGGA GGAAAGGAGA AGAAAAGCAA AGAACGAGAC 
AAAGGAAAGA AGAGGAAACA TCTCAGACAG AAACGAGACG AAAGTCCATC GTCCAGTGAA 
GAGGACAAGA AAAAGAGCAT TACGTTCAAG GATGAGGCAA AAAAAGCTGG AAAAGAAGAT 
GAGGAGGAAG AAGAATGGGA GCGGGAAGAG CGAGAGAGAC TTCAGGATCT GGAGGAGAGG 
GACGCCTTTG CTGAACGAGT GAAACAGAAG GATAAAGACA AGACGAGACA CATACTTGAA 
AGAAATGACA AAAAGGCGTA TGAGGAAGCT CAGAAAAGGC TCAAAATGGC TGAAGAGGAC 
CAAAGGAAAA TGCTTCCAGA GTTGAGGAAA CAGTCACGTC GGCAGTATCT GTCCAAAAGA 
GAGCAGGAGA AGCTGGAAGA CCTGGAGGCT GAAATTAAGG ATGAGGAGTA CCTCTTCTCC 
ACTCAAAACC TCACTGATCG GGAGAGGAAA GATTTGGAGT ACAAGCGGAC CATTAGAGAC 
ATGGCCAAGG AATACAAGAA AGCGGGAGCG AAAGAACAAG AGGAGAGAAA GAACAGATAC 
TACATGCCAG AAGAGAAAAG AAATAAGTCG ATTCCTCAAA AAGACATGGA TCTGGAGTTT 
GAGGAGACTC CCAGAGAGGG CGGTGGAGAG CAGGGCCGAT GGGAGGAGGC ACGAGTGGCC 
ACTGCCACAC TGCAGTTTGG GGCCAGACAA GAGCGCGAAC GGCGGATCAA GGAGGAGCAG 
GAGAGATACC AGCTCATTTT AGAGGAAGAG GAGATGATCA CCTTCGTCAG CACCGCAATT 
ACCATGAAGG GAACGCTGTC AGAGAAGGAA AGTGAACCTG AGTTATCACA AGCTGAGAAA 
CAGAAGCAGT CCATCCAGGA GGTGAGACGC AGCCTTCCCA TCTTTCCCTA CAGAGAAGAC 
CTGCTAGCAG CCATCGGAGA CCACCAGATC CTGGTCATAG AGGGTGAAAC CGGCTCTGGG 
AAGACGACCC AGATCCCTCA GTATCTCCTT GAAGAGGGTT ACACAAAAGG AGGAATGAAG 
ATCGGCTGTA CTCAACCTCG AAGAGTGGCA GCCATGTCCG TAGCAGCCAG AGTTGCACAG 
GAAATGAGCG TCAAACTAGG AAATGAGGTG GGCTACAGCA TCCGTTTTGA GGACTGTACA 
TCAGAGCGCA CCATTCTTAA GTATATGACT GACGGTATGC TCCTCAGAGA GTTTCTTACA 
GAGCCTGACC TCGCCAGCTA CAGTGTGATC ATCATTGATG AGGCTCATGA GCGAACGTTA 
CACACAGACA TCCTCTTCGG TCTGATTAAA GACATTGCAC GTTTCCGCCC TGACCTGAAG 
GTCCTGGTTG CCAGCGCCAC ACTTGACACA GAACGCTTCT CCAGCTTTTT TGATGATGCA 
CCTGTGTTTA GGATTCCAGG CCGCAGGTTC CCTGTGGACA TATATTACAC AAAGGCTCCA 
GAAGCCGACT ATCTGGAAGC ATGTGTGGTG TCAGTGCTAC AGATCCACGT CACTCAGTCG 
CCTGGAGACG TACTGGTCTT CCTCACGGGA CAGGAGGAGA TCGAGGCATG CTGTGAACTT 
CTACAGGAGA GATGTAGACG ACTGGGTTCA AAGATATCTG AGCTTCTTGT TCTGCCCATC 
TATGCCAACC TGCCCTCAGA CATGCAAGCC AAGATCTTCA ACCCCACTCC GCCTGGAGCA 
CGCAAAGTTG TAGTGGCCAC AAATATCGCA GAGACATCTC TCACCATCGA CGGCATCATC 
TATGTGATCG ATCCGGGATT TTGCAAGCAA AAGAGCTACA ATGCAAAAAC AGGAATGGAG 
TCTCTCATTG TCACTCCTTG TTCACGGGCC TCAGCCAATC AGAGAGCTGG CCGTGCAGGC 
AGAGTGGCTG CTGGGAAATG TTTTCGGCTG TACACTGCCT GGGCGTTTAA ACATGAGATG 
GAGGAGACCA CTGTTCCTGA GATCCAGAGA ACAAACCTGG GCAATGTGGT TCTGCTGCTC 
AAAAGTCTTG GCATAAATGA CCTGATTCAT TTTGACTTCA TGGATCCTCC TCCTCATGAA 
ACTCTGGTGT TGGCTTTGGA GCAGCTCTAT GCTTTAGGCG CCCTCAATCA CCTCGGCGAA 
CTCACTAAGC TTGGCCGAAG GATGGCTGAA CTTCCTGTTG ACCCGATGCT GAGTAAAATG 
ATTCTGGCTT CTGAACAATA TAAGTGCTCT GAGGAGGTGT TGACCATTGC GGCCATGCTG 
TCAGTAAACA ACTCCATCTT CTACCGGCCC AAGGACAAAG TGGTCCATGC CGACAACGCT 
CGGATGAACT TTGTGGTACC AGGAGGAGAT CACCTAGTGC TGCTTAATGT ATATACACAG 
TGGGTCGAAA GCGGTTACTC TACTCAGTGG TGCTTTGAAA ACTTCATTCA GTTTCGCTCC 
ATGAAGAGAG CTCGAGATGT CCGAGATCAG CTGGAGGGGT TGATGGACAG GATCGAGGTG 
GAGCTGTGCA GCTCCAATGG CGACAGCATG CCCATTCGCA AGGCAGTAAC TGCGGGATAT 
TTCTACCATA CAGCACGGTT GAGTAAAGGG GGATATAAAA CGGTAAAACA CCAGCAGACA 
GTTTACGTCC ATCCCAACAG CTCACTGTTT GAGGAGCAGC CGCGATGGAT GATCTACCAT 
GAGCTGGTCT TCACCACCAA AGAGTTCATG AGACAGGTGA TCGAGATTGA AAGCGGCTGG 
CTGCTGGAAG TGGCTCCTCA CTATTACAAG AACAAAGAGC TGGAGGACAG CAGCAGCAAG 
AAAATGCCCC GAAAACAAGG CAAAGCCCGC GAAGAACTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214709.1
CDS	21..3185
Translation	MANLEQWVSDQLHDILGLSDRYVAQFMIGLVQKSSGPQDFVSRLQDTGTIDIDQRITAFAHELYDKAPKKHVVEKPARAIERQVMEMERKNRTYTLLEDSESDGEAAREGGKEKKSKERDKGKKRKHLRQKRDESPSSSEEDKKKSITFKDEAKKAGKEDEEEEEWEREERERLQDLEERDAFAERVKQKDKDKTRHILERNDKKAYEEAQKRLKMAEEDQRKMLPELRKQSRRQYLSKREQEKLEDLEAEIKDEEYLFSTQNLTDRERKDLEYKRTIRDMAKEYKKAGAKEQEERKNRYYMPEEKRNKSIPQKDMDLEFEETPREGGGEQGRWEEARVATATLQFGARQERERRIKEEQERYQLILEEEEMITFVSTAITMKGTLSEKESEPELSQAEKQKQSIQEVRRSLPIFPYREDLLAAIGDHQILVIEGETGSGKTTQIPQYLLEEGYTKGGMKIGCTQPRRVAAMSVAARVAQEMSVKLGNEVGYSIRFEDCTSERTILKYMTDGMLLREFLTEPDLASYSVIIIDEAHERTLHTDILFGLIKDIARFRPDLKVLVASATLDTERFSSFFDDAPVFRIPGRRFPVDIYYTKAPEADYLEACVVSVLQIHVTQSPGDVLVFLTGQEEIEACCELLQERCRRLGSKISELLVLPIYANLPSDMQAKIFNPTPPGARKVVVATNIAETSLTIDGIIYVIDPGFCKQKSYNAKTGMESLIVTPCSRASANQRAGRAGRVAAGKCFRLYTAWAFKHEMEETTVPEIQRTNLGNVVLLLKSLGINDLIHFDFMDPPPHETLVLALEQLYALGALNHLGELTKLGRRMAELPVDPMLSKMILASEQYKCSEEVLTIAAMLSVNNSIFYRPKDKVVHADNARMNFVVPGGDHLVLLNVYTQWVESGYSTQWCFENFIQFRSMKRARDVRDQLEGLMDRIEVELCSSNGDSMPIRKAVTAGYFYHTARLSKGGYKTVKHQQTVYVHPNSSLFEEQPRWMIYHELVFTTKEFMRQVIEIESGWLLEVAPHYYKNKELEDSSSKKMPRKQGKAREELG

Target ORF information:

RefSeq Version	XM_017359220.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359220.1

ORF Insert Sequence:

ATGGCCAATC TGGAACAGTG GGTTAGTGAC CAGCTCCATG ACATCCTGGG TTTAAGTGAC 
CGATATGTGG CCCAGTTCAT GATCGGACTG GTTCAGAAGT CGTCTGGACC TCAGGATTTT 
GTGTCTCGGC TTCAGGACAC TGGAACTATT GACATTGATC AAAGAATCAC AGCGTTTGCA 
CATGAGTTGT ACGACAAGGC TCCCAAAAAG CATGTGGTTG AAAAACCTGC TCGAGCAATC 
GAACGTCAGG TCATGGAGAT GGAGAGAAAG AACAGAACCT ACACCCTTCT GGAGGACAGC 
GAGAGCGATG GAGAGGCAGC CAGAGAAGGA GGAAAGGAGA AGAAAAGCAA AGAACGAGAC 
AAAGGAAAGA AGAGGAAACA TCTCAGACAG AAACGAGACG AAAGTCCATC GTCCAGTGAA 
GAGGACAAGA AAAAGAGCAT TACGTTCAAG GATGAGGCAA AAAAAGCTGG AAAAGAAGAT 
GAGGAGGAAG AAGAATGGGA GCGGGAAGAG CGAGAGAGAC TTCAGGATCT GGAGGAGAGG 
GACGCCTTTG CTGAACGAGT GAAACAGAAG GATAAAGACA AGACGAGACA CATACTTGAA 
AGAAATGACA AAAAGGCGTA TGAGGAAGCT CAGAAAAGGC TCAAAATGGC TGAAGAGGAC 
CAAAGGAAAA TGCTTCCAGA GTTGAGGAAA CAGTCACGTC GGCAGTATCT GTCCAAAAGA 
GAGCAGGAGA AGCTGGAAGA CCTGGAGGCT GAAATTAAGG ATGAGGAGTA CCTCTTCTCC 
ACTCAAAACC TCACTGATCG GGAGAGGAAA GATTTGGAGT ACAAGCGGAC CATTAGAGAC 
ATGGCCAAGG AATACAAGAA AGCGGGAGCG AAAGAACAAG AGGAGAGAAA GAACAGATAC 
TACATGCCAG AAGAGAAAAG AAATAAGTCG ATTCCTCAAA AAGACATGGA TCTGGAGTTT 
GAGGAGACTC CCAGAGAGGG CGGTGGAGAG CAGGGCCGAT GGGAGGAGGC ACGAGTGGCC 
ACTGCCACAC TGCAGTTTGG GGCCAGACAA GAGCGCGAAC GGCGGATCAA GGAGGAGCAG 
GAGAGATACC AGCTCATTTT AGAGGAAGAG GAGATGATCA CCTTCGTCAG CACCGCAATT 
ACCATGAAGG GAACGCTGTC AGAGAAGGAA AGTGAACCTG AGTTATCACA AGCTGAGAAA 
CAGAAGCAGT CCATCCAGGA GGTGAGACGC AGCCTTCCCA TCTTTCCCTA CAGAGAAGAC 
CTGCTAGCAG CCATCGGAGA CCACCAGATC CTGGTCATAG AGGGTGAAAC CGGCTCTGGG 
AAGACGACCC AGATCCCTCA GTATCTCCTT GAAGAGGGTT ACACAAAAGG AGGAATGAAG 
ATCGGCTGTA CTCAACCTCG AAGAGTGGCA GCCATGTCCG TAGCAGCCAG AGTTGCACAG 
GAAATGAGCG TCAAACTAGG AAATGAGGTG GGCTACAGCA TCCGTTTTGA GGACTGTACA 
TCAGAGCGCA CCATTCTTAA GTATATGACT GACGGTATGC TCCTCAGAGA GTTTCTTACA 
GAGCCTGACC TCGCCAGCTA CAGTGTGATC ATCATTGATG AGGCTCATGA GCGAACGTTA 
CACACAGACA TCCTCTTCGG TCTGATTAAA GACATTGCAC GTTTCCGCCC TGACCTGAAG 
GTCCTGGTTG CCAGCGCCAC ACTTGACACA GAACGCTTCT CCAGCTTTTT TGATGATGCA 
CCTGTGTTTA GGATTCCAGG CCGCAGGTTC CCTGTGGACA TATATTACAC AAAGGCTCCA 
GAAGCCGACT ATCTGGAAGC ATGTGTGGTG TCAGTGCTAC AGATCCACGT CACTCAGTCG 
CCTGGAGACG TACTGGTCTT CCTCACGGGA CAGGAGGAGA TCGAGGCATG CTGTGAACTT 
CTACAGGAGA GATGTAGACG ACTGGGTTCA AAGATATCTG AGCTTCTTGT TCTGCCCATC 
TATGCCAACC TGCCCTCAGA CATGCAAGCC AAGATCTTCA ACCCCACTCC GCCTGGAGCA 
CGCAAAGTTG TAGTGGCCAC AAATATCGCA GAGACATCTC TCACCATCGA CGGCATCATC 
TATGTGATCG ATCCGGGATT TTGCAAGCAA AAGAGCTACA ATGCAAAAAC AGGAATGGAG 
TCTCTCATTG TCACTCCTTG TTCACGGGCC TCAGCCAATC AGAGAGCTGG CCGTGCAGGC 
AGAGTGGCTG CTGGGAAATG TTTTCGGCTG TACACTGCCT GGGCGTTTAA ACATGAGATG 
GAGGAGACCA CTGTTCCTGA GATCCAGAGA ACAAACCTGG GCAATGTGGT TCTGCTGCTC 
AAAAGTCTTG GCATAAATGA CCTGATTCAT TTTGACTTCA TGGATCCTCC TCCTCATGAA 
ACTCTGGTGT TGGCTTTGGA GCAGCTCTAT GCTTTAGGCG CCCTCAATCA CCTCGGCGAA 
CTCACTAAGC TTGGCCGAAG GATGGCTGAA CTTCCTGTTG ACCCGATGCT GAGTAAAATG 
ATTCTGGCTT CTGAACAATA TAAGTGCTCT GAGGAGGTGT TGACCATTGC GGCCATGCTG 
TCAGTAAACA ACTCCATCTT CTACCGGCCC AAGGACAAAG TGGTCCATGC CGACAACGCT 
CGGATGAACT TTGTGGTACC AGGAGGAGAT CACCTAGTGC TGCTTAATGT ATATACACAG 
TGGGTCGAAA GCGGTTACTC TACTCAGTGG TGCTTTGAAA ACTTCATTCA GTTTCGCTCC 
ATGAAGAGAG CTCGAGATGT CCGAGATCAG CTGGAGGGGT TGATGGACAG GATCGAGGTG 
GAGCTGTGCA GCTCCAATGG CGACAGCATG CCCATTCGCA AGGCAGTAAC TGCGGGATAT 
TTCTACCATA CAGCACGGTT GAGTAAAGGG GGATATAAAA CGGTAAAACA CCAGCAGACA 
GTTTACGTCC ATCCCAACAG CTCACTGTTT GAGGAGCAGC CGCGATGGAT GATCTACCAT 
GAGCTGGTCT TCACCACCAA AGAGTTCATG AGACAGGTGA TCGAGATTGA AAGCGGCTGG 
CTGCTGGAAG TGGCTCCTCA CTATTACAAG AACAAAGAGC TGGAGGACAG CAGCAGCAAG 
AAAATGCCCC GAAAACAAGG CAAAGCCCGC GAAGAACTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX16 ( NM_200024.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_956318.1 Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), mRNA.

Price & Availability↑

CloneID	ODa36296
Clone ID Related Accession (Same CDS sequence)	NM_200024.1
Accession Version	NM_200024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045393.1. ##Evidence-Data-START## Transcript exon combination :: BC045393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAATC TGGAACAGTG GGTTAGTGAC CAGCTCCATG ACATCCTGGG TTTAAGTGAC 
CGATATGTGG CCCAGTTCAT GATCGGACTG GTTCAGAAGT CGTCTGGACC TCAGGATTTT 
GTGTCTCGGC TTCAGGACAC TGGAACTATT GACATTGATC AAAGAATCAC AGCGTTTGCA 
CATGAGTTGT ACGACAAGGC TCCCAAAAAG CATGTGGTTG AAAAACCTGC TCGAGCAATC 
GAACGTCAGG TCATGGAGAT GGAGAGAAAG AACAGAACCT ACACCCTTCT GGAGGACAGC 
GAGAGCGATG GAGAGGCAGC CAGAGAAGGA GGAAAGGAGA AGAAAAGCAA AGAACGAGAC 
AAAGGAAAGA AGAGGAAACA TCTCAGACAG AAACGAGACG AAAGTCCATC GTCCAGTGAA 
GAGGACAAGA AAAAGAGCAT TACGTTCAAG GATGAGCCAA AAAAAGCTGG AAAAGAAGAT 
GAGGAGGAAG AAGAATGGGA GCGGGGAGAG CGAGAGAGAC TTCAGGATCT GGAGGAGAGG 
GACGCCTTTG CTGAACGAGT GAAACAGAAG GATAAAGACA AGACGAGAAA CATACTTGAA 
AGAAATGACA AAAAGGCGTA TGAGGAAGCT CAGAAAAGGA TCAAAATGGC TGAAGAGGAC 
AAAAGGAAAA TGCTTCCAGA GTTGAGGAAA CAGTCACGTC GGCAGTATCT GTCCAAAAGA 
GAGCAGGAGA AGCTGGAAGA CCTGGAGGCT GAAATTAAGG ATGAGGAGTA CCTCTTCTCC 
ACTCAAAACC TCACTGATCG GGAGAGGAAA GATTTGGAGT ACAAGCGGAC CATTAGAGAC 
ATGGCCAAGG AATACAAGAA AGCGGGAGCG AAAGAACAAG AGGAGAGAAA GAACAGATAC 
TACATGCCAG AAGAGAAAAG AAATAAGTCG ATTCCTCAAA AAGACATGGA TCTGGAGTTT 
GAGGAGACTC CCAGAGAGGG CGGTGGAGAG CAGGGCCGAT GGGAGGAGGC ACGAGTGGCC 
ACTGCCACAC TGCAGTTTGG GGCCAGACAA GAGCGCGAAC GGCGGATCAA GGAGGAGCAG 
GAGAGATACC AGCTCATTTT AGAGGAAGAG GAGATGATCA CCTTCGTCAG CACCGCAATT 
ACCATGAAGG GAACGCTGTC AGAGAAGGAA AGTGAACCTG AGTTATCACA AGCTGAGAAA 
CAGAAGCAGT CCATCCAGGA GGTGAGACGC AGCCTTCCCA TCTTTCCCTA CAGAGAAGAC 
CTGCTAGCAG CCATCGGAGA CCACCAGATC CTGGTCATAG AGGGTGAAAC CGGCTCTGGG 
AAGACGACCC AGATCCCTCA GTATCTCCTT GAAGAGGGTT ACACAAAAGG AGGAATGAAG 
ATCGGCTGTA CTCAACCTCG AAGAGTGGCG GCCATGTCCG TAGCAGCCAG AGTTGCACAG 
GAAATGAGCG TCAAACTAGG AAATGAGGTG GGCTACAGCA TCCGTTTTGA GGACTGTACA 
TCAGAGCGCA CCATTCTTAA GTATATGACT GACGGTATGC TCCTCAGAGA GTTTCTTACA 
GAGCCTGACC TCGCCAGCTA CAGTGTGATC ATCATTGATG AGGCTCATGA GCGAACGTTA 
CACACAGACA TCCTCTTCGG TCTGATTAAA GACATTGCAC GTTTCCGCCC TGACCTGAAG 
GTCCTGGTTG CCAGCGCCAC ACTTGACACA GAACGCTTCT CCAGCTTTTT TGATGATGCA 
CCTGTGTTTA GGATTCCAGG CCGCAGGTTC CCTGTGGACA TATATTACAC AAAGGCTCCA 
GAAGCCGACT ATCTTGAAGC ATGTGTGGTG TCAGTGCTAC AGATCCACGT CACTCAGTCG 
CCTGGAGACG TACTGGTCTT CCTCACGGGA CAGGAGGAGA TCGAGGCATG CTGTGAACTT 
CTACAGGAGA GATGTAGACG ACTGGGTTCA AAGATATCTG AGCTTCTTGT TCTGCCCATC 
TATGCCAACC TGCCCTCAGA CATGCAAGCC AAGATCTTCA ACCCCACTCC GCCTGGAGCA 
CGCAAAGTTG TAGTGGCCAC AAATATCGCA GAGACATCTC TCACCATCGA CGGCATCATC 
TATGTGATCG ATCCGGGATT TTGTAAGCAA AAGAGCTACA ATGCAAAAAC AGGAATGGAG 
TCTCTCATTG TCACTCCTTG TTCACGGGCC TCAGCCAATC AGAGAGCTGG CCGTGCAGGC 
AGAGTGGCTG CTGGGAAATG TTTTCGGCTG TACACTGCCT GGGCGTTTAA ACATGAGATG 
GAGGAGACCA CTGTTCCTGA GATCCAGAGA ACAAACCTGG GCAATGTGGT TCTGCTGCTC 
AAAAGTCTTG GCATAAATGA CCTGATTCAT TTTGACTTCA TGGATCCTCC TCCTCATGAA 
ACTCTGGTGT TGGCTTTGGA GCAGCTCTAT GCTTTAGGCG CCCTCAATCA CCTCGGCGAA 
CTCACTAAGC TTGGCCGAAG GATGGCTGAA CTTCCTGTTG ACCCGATGCT GAGTAAAATG 
ATTCTGGCTT CTGAACAATA TAAGTGCTCT GAGGAGGTGT TGACCATTGC GGCCATGCTG 
TCAGTAAACA ACTCCATCTT CTACCGGCCC AAGGACAAAG TGGTCCATGC CGACAACGCT 
CGGATGAACT TTGTGGTACC AGGAGGAGAT CACCTAGTGC TGCTTAATGT ATATACACAG 
TGGGTCGAAA GCGGTTACTC TACTCAGTGG TGCTTTGAAA ACTTCATTCA GTTTCGCTCC 
ATGAAGAGAG CTCGAGATGT CCGAGATCAG CTGGAGGGGT TGATGGACAG GATCGAGGTG 
GAGCTGTGCA GCTCCAATGG CGACAGCATG CCCATTCGCA AGGCAGTAAC TGCGGGGTAT 
TTCTACCATA CAGCACGGTT GAGTAAAGGG GGATATAAAA CGGTAAAACA CCAGCAGACA 
GTTTACGTCC ATCCCAACAG CTCACTGTTT GAGGAGCAGC CGCGATGGAT GATCTACCAT 
GAGCTGGTCT TCACCACCAA AGAGTTCATG AGACAGGTGA TCGAGATTGA AAGCGGCTGG 
CTGCTGGAAG TGGCTCCTCA CTATTACAAG AACAAAGAGC TGGAGGACAG CAGCAGCAAG 
AAAATGCCCC GAAAACAAGG CAAAGCCCGC GAAGAACTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956318.1
CDS	81..3245
Translation	MANLEQWVSDQLHDILGLSDRYVAQFMIGLVQKSSGPQDFVSRLQDTGTIDIDQRITAFAHELYDKAPKKHVVEKPARAIERQVMEMERKNRTYTLLEDSESDGEAAREGGKEKKSKERDKGKKRKHLRQKRDESPSSSEEDKKKSITFKDEPKKAGKEDEEEEEWERGERERLQDLEERDAFAERVKQKDKDKTRNILERNDKKAYEEAQKRIKMAEEDKRKMLPELRKQSRRQYLSKREQEKLEDLEAEIKDEEYLFSTQNLTDRERKDLEYKRTIRDMAKEYKKAGAKEQEERKNRYYMPEEKRNKSIPQKDMDLEFEETPREGGGEQGRWEEARVATATLQFGARQERERRIKEEQERYQLILEEEEMITFVSTAITMKGTLSEKESEPELSQAEKQKQSIQEVRRSLPIFPYREDLLAAIGDHQILVIEGETGSGKTTQIPQYLLEEGYTKGGMKIGCTQPRRVAAMSVAARVAQEMSVKLGNEVGYSIRFEDCTSERTILKYMTDGMLLREFLTEPDLASYSVIIIDEAHERTLHTDILFGLIKDIARFRPDLKVLVASATLDTERFSSFFDDAPVFRIPGRRFPVDIYYTKAPEADYLEACVVSVLQIHVTQSPGDVLVFLTGQEEIEACCELLQERCRRLGSKISELLVLPIYANLPSDMQAKIFNPTPPGARKVVVATNIAETSLTIDGIIYVIDPGFCKQKSYNAKTGMESLIVTPCSRASANQRAGRAGRVAAGKCFRLYTAWAFKHEMEETTVPEIQRTNLGNVVLLLKSLGINDLIHFDFMDPPPHETLVLALEQLYALGALNHLGELTKLGRRMAELPVDPMLSKMILASEQYKCSEEVLTIAAMLSVNNSIFYRPKDKVVHADNARMNFVVPGGDHLVLLNVYTQWVESGYSTQWCFENFIQFRSMKRARDVRDQLEGLMDRIEVELCSSNGDSMPIRKAVTAGYFYHTARLSKGGYKTVKHQQTVYVHPNSSLFEEQPRWMIYHELVFTTKEFMRQVIEIESGWLLEVAPHYYKNKELEDSSSKKMPRKQGKAREELG

Target ORF information:

RefSeq Version	NM_200024.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAH (Asp-Glu-Ala-His) box polypeptide 16 (dhx16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200024.1

ORF Insert Sequence:

ATGGCTAATC TGGAACAGTG GGTTAGTGAC CAGCTCCATG ACATCCTGGG TTTAAGTGAC 
CGATATGTGG CCCAGTTCAT GATCGGACTG GTTCAGAAGT CGTCTGGACC TCAGGATTTT 
GTGTCTCGGC TTCAGGACAC TGGAACTATT GACATTGATC AAAGAATCAC AGCGTTTGCA 
CATGAGTTGT ACGACAAGGC TCCCAAAAAG CATGTGGTTG AAAAACCTGC TCGAGCAATC 
GAACGTCAGG TCATGGAGAT GGAGAGAAAG AACAGAACCT ACACCCTTCT GGAGGACAGC 
GAGAGCGATG GAGAGGCAGC CAGAGAAGGA GGAAAGGAGA AGAAAAGCAA AGAACGAGAC 
AAAGGAAAGA AGAGGAAACA TCTCAGACAG AAACGAGACG AAAGTCCATC GTCCAGTGAA 
GAGGACAAGA AAAAGAGCAT TACGTTCAAG GATGAGCCAA AAAAAGCTGG AAAAGAAGAT 
GAGGAGGAAG AAGAATGGGA GCGGGGAGAG CGAGAGAGAC TTCAGGATCT GGAGGAGAGG 
GACGCCTTTG CTGAACGAGT GAAACAGAAG GATAAAGACA AGACGAGAAA CATACTTGAA 
AGAAATGACA AAAAGGCGTA TGAGGAAGCT CAGAAAAGGA TCAAAATGGC TGAAGAGGAC 
AAAAGGAAAA TGCTTCCAGA GTTGAGGAAA CAGTCACGTC GGCAGTATCT GTCCAAAAGA 
GAGCAGGAGA AGCTGGAAGA CCTGGAGGCT GAAATTAAGG ATGAGGAGTA CCTCTTCTCC 
ACTCAAAACC TCACTGATCG GGAGAGGAAA GATTTGGAGT ACAAGCGGAC CATTAGAGAC 
ATGGCCAAGG AATACAAGAA AGCGGGAGCG AAAGAACAAG AGGAGAGAAA GAACAGATAC 
TACATGCCAG AAGAGAAAAG AAATAAGTCG ATTCCTCAAA AAGACATGGA TCTGGAGTTT 
GAGGAGACTC CCAGAGAGGG CGGTGGAGAG CAGGGCCGAT GGGAGGAGGC ACGAGTGGCC 
ACTGCCACAC TGCAGTTTGG GGCCAGACAA GAGCGCGAAC GGCGGATCAA GGAGGAGCAG 
GAGAGATACC AGCTCATTTT AGAGGAAGAG GAGATGATCA CCTTCGTCAG CACCGCAATT 
ACCATGAAGG GAACGCTGTC AGAGAAGGAA AGTGAACCTG AGTTATCACA AGCTGAGAAA 
CAGAAGCAGT CCATCCAGGA GGTGAGACGC AGCCTTCCCA TCTTTCCCTA CAGAGAAGAC 
CTGCTAGCAG CCATCGGAGA CCACCAGATC CTGGTCATAG AGGGTGAAAC CGGCTCTGGG 
AAGACGACCC AGATCCCTCA GTATCTCCTT GAAGAGGGTT ACACAAAAGG AGGAATGAAG 
ATCGGCTGTA CTCAACCTCG AAGAGTGGCG GCCATGTCCG TAGCAGCCAG AGTTGCACAG 
GAAATGAGCG TCAAACTAGG AAATGAGGTG GGCTACAGCA TCCGTTTTGA GGACTGTACA 
TCAGAGCGCA CCATTCTTAA GTATATGACT GACGGTATGC TCCTCAGAGA GTTTCTTACA 
GAGCCTGACC TCGCCAGCTA CAGTGTGATC ATCATTGATG AGGCTCATGA GCGAACGTTA 
CACACAGACA TCCTCTTCGG TCTGATTAAA GACATTGCAC GTTTCCGCCC TGACCTGAAG 
GTCCTGGTTG CCAGCGCCAC ACTTGACACA GAACGCTTCT CCAGCTTTTT TGATGATGCA 
CCTGTGTTTA GGATTCCAGG CCGCAGGTTC CCTGTGGACA TATATTACAC AAAGGCTCCA 
GAAGCCGACT ATCTTGAAGC ATGTGTGGTG TCAGTGCTAC AGATCCACGT CACTCAGTCG 
CCTGGAGACG TACTGGTCTT CCTCACGGGA CAGGAGGAGA TCGAGGCATG CTGTGAACTT 
CTACAGGAGA GATGTAGACG ACTGGGTTCA AAGATATCTG AGCTTCTTGT TCTGCCCATC 
TATGCCAACC TGCCCTCAGA CATGCAAGCC AAGATCTTCA ACCCCACTCC GCCTGGAGCA 
CGCAAAGTTG TAGTGGCCAC AAATATCGCA GAGACATCTC TCACCATCGA CGGCATCATC 
TATGTGATCG ATCCGGGATT TTGTAAGCAA AAGAGCTACA ATGCAAAAAC AGGAATGGAG 
TCTCTCATTG TCACTCCTTG TTCACGGGCC TCAGCCAATC AGAGAGCTGG CCGTGCAGGC 
AGAGTGGCTG CTGGGAAATG TTTTCGGCTG TACACTGCCT GGGCGTTTAA ACATGAGATG 
GAGGAGACCA CTGTTCCTGA GATCCAGAGA ACAAACCTGG GCAATGTGGT TCTGCTGCTC 
AAAAGTCTTG GCATAAATGA CCTGATTCAT TTTGACTTCA TGGATCCTCC TCCTCATGAA 
ACTCTGGTGT TGGCTTTGGA GCAGCTCTAT GCTTTAGGCG CCCTCAATCA CCTCGGCGAA 
CTCACTAAGC TTGGCCGAAG GATGGCTGAA CTTCCTGTTG ACCCGATGCT GAGTAAAATG 
ATTCTGGCTT CTGAACAATA TAAGTGCTCT GAGGAGGTGT TGACCATTGC GGCCATGCTG 
TCAGTAAACA ACTCCATCTT CTACCGGCCC AAGGACAAAG TGGTCCATGC CGACAACGCT 
CGGATGAACT TTGTGGTACC AGGAGGAGAT CACCTAGTGC TGCTTAATGT ATATACACAG 
TGGGTCGAAA GCGGTTACTC TACTCAGTGG TGCTTTGAAA ACTTCATTCA GTTTCGCTCC 
ATGAAGAGAG CTCGAGATGT CCGAGATCAG CTGGAGGGGT TGATGGACAG GATCGAGGTG 
GAGCTGTGCA GCTCCAATGG CGACAGCATG CCCATTCGCA AGGCAGTAAC TGCGGGGTAT 
TTCTACCATA CAGCACGGTT GAGTAAAGGG GGATATAAAA CGGTAAAACA CCAGCAGACA 
GTTTACGTCC ATCCCAACAG CTCACTGTTT GAGGAGCAGC CGCGATGGAT GATCTACCAT 
GAGCTGGTCT TCACCACCAA AGAGTTCATG AGACAGGTGA TCGAGATTGA AAGCGGCTGG 
CTGCTGGAAG TGGCTCCTCA CTATTACAAG AACAAAGAGC TGGAGGACAG CAGCAGCAAG 
AAAATGCCCC GAAAACAAGG CAAAGCCCGC GAAGAACTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes.
Developmental biology353(2)275-89(2011 May)
Putiri E,Pelegri F

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
BMC genomics6152(2005 Nov)
Sambrook JG,Figueroa F,Beck S

Identification of recessive maternal-effect mutations in the zebrafish using a gynogenesis-based method.
Developmental dynamics : an official publication of the American Association of Anatomists231(2)324-35(2004 Oct)
Pelegri F,Dekens MP,Schulte-Merker S,Maischein HM,Weiler C,N?sslein-Volhard C

Identification of 315 genes essential for early zebrafish development.
Proceedings of the National Academy of Sciences of the United States of America101(35)12792-7(2004 Aug)
Amsterdam A,Nissen RM,Sun Z,Swindell EC,Farrington S,Hopkins N

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DHX16 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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