ppp4r3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppp4r3b
Entrez Gene ID	336198
Full Name	protein phosphatase 4, regulatory subunit 3B
Synonyms	flfl,smek2,wu:fj55c11,zgc:56492,zgc:77352
General protein information	Preferred Names protein phosphatase 4, regulatory subunit 3B Names serine/threonine-protein phosphatase 4 regulatory subunit 3B SMEK homolog 2, suppressor of mek1 falafel
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200001.2	NP_956295.2	serine/threonine-protein phosphatase 4 regulatory subunit 3B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMEK2	XP_003309065.1
Bos taurus (cattle)	SMEK2	NP_001178079.1
Gallus gallus (chicken)	SMEK2	XP_424305.3
Drosophila melanogaster (fruit fly)	flfl	NP_731849.2
Canis lupus familiaris (dog)	SMEK2	XP_005626173.1
	SMEK2	XP_001112386.2
Rattus norvegicus (Norway rat)	Smek2	NP_001101837.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003402	XP_311690.5
Homo sapiens (human)	SMEK2	NP_001116436.2
Mus musculus (house mouse)	Smek2	NP_598795.2
Danio rerio (zebrafish)	smek2	NP_956295.2
Xenopus tropicalis (tropical clawed frog)	smek2	NP_001005004.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppp4r3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppp4r3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05017	NM_200001.2 Latest version!	Danio rerio protein phosphatase 4, regulatory subunit 3B (ppp4r3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppp4r3b ( NM_200001.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956295.2 Danio rerio protein phosphatase 4, regulatory subunit 3B (ppp4r3b), mRNA.

Price & Availability↑

CloneID	ODa05017
Clone ID Related Accession (Same CDS sequence)	NM_200001.2
Accession Version	NM_200001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065348.1. On Apr 25, 2004 this sequence version replaced NM_200001.1. ##Evidence-Data-START## Transcript exon combination :: BC065348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGACA CCCGACGGCG CGTGAAGGTT TACACGCTGA ATGAAGAGCG GCAGTGGGAG 
GACAGAGGAA CCGGCCATGT GTGCTGCTCC GCCGAGGACA CCGCGCTGTC GGTCACCGCA 
GAGTCTGACG GGTCACTGCT GCTCGAGTCC AAGATCAAGC CCAGCACTGC CTATCAGAAA 
CAGCAGGACA CACTGATCGT CTGGTCTGAA GCGGAGAACT ATGATCTGGC TCTGAGTTTC 
CAGGAGAAGG CCGGCTGTGA CGAGATCTGG GAGAAGATCT GTCAGGTCCA AGGCAAGGAC 
CCGTCAGTGG AGATCACGCA GGAGCCGGTG GATGAGTCTG AGGAGGAGCG CTGTGAGGAC 
TCTGGAGCGG AGTGTGACGC TCTGGATCTG CCCGAGTGTG TGCCGGAGCG TCTGCAGGAG 
CTGGCGGAGC TGGTGTCGTC GGTGCTGCCG TCGCCGGTGC GCAGGGAGAA GCTGGCGCTG 
GCGCTGGTGA GCGGCGGGTA CCTGCGGCAG CTGCTGCTGC TGTTCCGGCG CTGTGAGGAG 
CAGGAGAACC GGCCGCGGCT GCAGCAGCTC TTCCAGATCC TGCGCGGGCT GCTGTTCCTC 
AATAAGGCGG CGCTGTTCGA GGTGCTGTTC TCCGACGACT GCATCATGGA CGTGGTGGGC 
TGCCTGGAGT ACGACCCCGC GCTGCTGCAG CCCAAACGCC ACCGGGACTT CCTCACCAAG 
ACGGCGCGCT TCCGGGAGGT CATCCCCATC CAGGACTCGG AGCTGCGGCA GAAGATCCAC 
CAGACCTACC GCGTGCAGTA CATCCAGGAC ATCATCCTGC CCACGCCCTC CGTCTTCGAG 
GAGAACTTCC TGTCCACGCT CTCCTCCTTC ATCTTCTTCA ACAAAGTGGA GATCGTCAGC 
ATGCTGCAGG AGGACGAGAA GTTTCTGACA GAGGTATTTG CTCAGCTCAC TGATGAATCC 
ACAGAGGACA GCAAGAGGAG AGAGCTGGTG AACTTCTTCA AGGAGTTCTG TGCGTTCTCG 
CAGACGCTGC AGCCACAGAA CAGAGACGCC TTCTTCAAGA CTCTGGCCAA TCTGGGCATC 
CTGCCGGCAC TGGAGATCGT GATGGGCATG GATGATGTGC AGGTGAAGGC CGCCGCCACA 
GATATCTTCT CCTACCTGGT GGAGTTCAGT CCATCGATGG TGCGTGAGTT CGTGATGCAG 
GAGCCGCAGC AGGCTGATGA TGACGTGCTG CTGATTAACG TGGTGATCAA GCAGATGATC 
TGTGATTCAG ACCCTGAGCT GGGCGGAGCC GTGCAGCTGA TGGGGCTCCT GCGCACACTC 
ATAGACCCCG AGAACATGCT CGCACCCTCC AACAAAACAG AGAAGAGTGA GTTTCTGAGC 
TTCTTCTACA AGTACTGTAT GCATGTGCTG ACGGCTCCAC TGCTGGCCAA CACCGCCGAG 
GAGGACCAAG ATTTGGATGA AGGATCAGCT AAAGTCAACC CTGTGTGTCC AGATAACTTC 
CAGACGGCGC AGCTGCTGGC GCTGATCCTG GAGCTGCTGA CCTTCTGTGT GGAGCATCAC 
ACGTACCACA TCAAGAACTA CATCATGACC AGAGACCTGC TGCGGAGGGT GCTGACGCTC 
ATGAACTCCA AACACACCTT CCTGGCGCTC TGTGCGCTGC GCTTCATGCG GAGGATCATC 
GGCCTGAAGG ATGAGTATTA TAACCGCTAC ATCATGAAGG GGAACCTGTT TGAGCCGGTC 
ATCAACGCTC TGCTGGACAA CGGCACGCGC TACAACCTGC TGAACTCCGC CATCATCGAG 
CTGTTCGACT TCATACGCGT GGAGGACATC CGCTCGCTCA CTGCACACAT TGTGGAGAAC 
TTCCAGAGCG CGCTGCAGTC CATCCAGTAT GTGCAGACCT TCAAGGGCCT GAAGGGGAGA 
TACGAGCAGG AGCAGGAGCG CCAGAGCCGG GGCCTCAGCA GGTTCCGCAG GGACGCGCGC 
TCCTTCCCGG AGGATGAAGA GCTGTGGAAG GAGGAAGAGG AGGATGAGGA GGAGCCAGCG 
GACACGGAGA CGCAACACTG CAAGAGCGAC TACGGGAGAT TCATGGAGAG CGAGAAAGCG 
AAAGAGAACC AGGAGAAGGA GAACCAGGCC AAGCGGAGCA GCAGCGGCAG CTTCAAGTTC 
ACCTTCTCCC ACTCAGCAGC GTCTGCTAAC AGCGCTAAAG CTACATCTGC GTCATCCAGC 
GGAGCCAGCA CTGCAGCCAG GACGGCGCTG GTGGATTACC CAGATGATGA AGATGAGGAT 
GAGGATGATG ATGAAGATCA GCAGGCTCCA CACAAGCGCC CCCGTCTGGG CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956295.2
CDS	272..2608
Translation	MSDTRRRVKVYTLNEERQWEDRGTGHVCCSAEDTALSVTAESDGSLLLESKIKPSTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEITQEPVDESEEERCEDSGAECDALDLPECVPERLQELAELVSSVLPSPVRREKLALALVSGGYLRQLLLLFRRCEEQENRPRLQQLFQILRGLLFLNKAALFEVLFSDDCIMDVVGCLEYDPALLQPKRHRDFLTKTARFREVIPIQDSELRQKIHQTYRVQYIQDIILPTPSVFEENFLSTLSSFIFFNKVEIVSMLQEDEKFLTEVFAQLTDESTEDSKRRELVNFFKEFCAFSQTLQPQNRDAFFKTLANLGILPALEIVMGMDDVQVKAAATDIFSYLVEFSPSMVREFVMQEPQQADDDVLLINVVIKQMICDSDPELGGAVQLMGLLRTLIDPENMLAPSNKTEKSEFLSFFYKYCMHVLTAPLLANTAEEDQDLDEGSAKVNPVCPDNFQTAQLLALILELLTFCVEHHTYHIKNYIMTRDLLRRVLTLMNSKHTFLALCALRFMRRIIGLKDEYYNRYIMKGNLFEPVINALLDNGTRYNLLNSAIIELFDFIRVEDIRSLTAHIVENFQSALQSIQYVQTFKGLKGRYEQEQERQSRGLSRFRRDARSFPEDEELWKEEEEDEEEPADTETQHCKSDYGRFMESEKAKENQEKENQAKRSSSGSFKFTFSHSAASANSAKATSASSSGASTAARTALVDYPDDEDEDEDDDEDQQAPHKRPRLGS

Target ORF information:

RefSeq Version	NM_200001.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase 4, regulatory subunit 3B (ppp4r3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200001.2

ORF Insert Sequence:

ATGAGCGACA CCCGACGGCG CGTGAAGGTT TACACGCTGA ATGAAGAGCG GCAGTGGGAG 
GACAGAGGAA CCGGCCATGT GTGCTGCTCC GCCGAGGACA CCGCGCTGTC GGTCACCGCA 
GAGTCTGACG GGTCACTGCT GCTCGAGTCC AAGATCAAGC CCAGCACTGC CTATCAGAAA 
CAGCAGGACA CACTGATCGT CTGGTCTGAA GCGGAGAACT ATGATCTGGC TCTGAGTTTC 
CAGGAGAAGG CCGGCTGTGA CGAGATCTGG GAGAAGATCT GTCAGGTCCA AGGCAAGGAC 
CCGTCAGTGG AGATCACGCA GGAGCCGGTG GATGAGTCTG AGGAGGAGCG CTGTGAGGAC 
TCTGGAGCGG AGTGTGACGC TCTGGATCTG CCCGAGTGTG TGCCGGAGCG TCTGCAGGAG 
CTGGCGGAGC TGGTGTCGTC GGTGCTGCCG TCGCCGGTGC GCAGGGAGAA GCTGGCGCTG 
GCGCTGGTGA GCGGCGGGTA CCTGCGGCAG CTGCTGCTGC TGTTCCGGCG CTGTGAGGAG 
CAGGAGAACC GGCCGCGGCT GCAGCAGCTC TTCCAGATCC TGCGCGGGCT GCTGTTCCTC 
AATAAGGCGG CGCTGTTCGA GGTGCTGTTC TCCGACGACT GCATCATGGA CGTGGTGGGC 
TGCCTGGAGT ACGACCCCGC GCTGCTGCAG CCCAAACGCC ACCGGGACTT CCTCACCAAG 
ACGGCGCGCT TCCGGGAGGT CATCCCCATC CAGGACTCGG AGCTGCGGCA GAAGATCCAC 
CAGACCTACC GCGTGCAGTA CATCCAGGAC ATCATCCTGC CCACGCCCTC CGTCTTCGAG 
GAGAACTTCC TGTCCACGCT CTCCTCCTTC ATCTTCTTCA ACAAAGTGGA GATCGTCAGC 
ATGCTGCAGG AGGACGAGAA GTTTCTGACA GAGGTATTTG CTCAGCTCAC TGATGAATCC 
ACAGAGGACA GCAAGAGGAG AGAGCTGGTG AACTTCTTCA AGGAGTTCTG TGCGTTCTCG 
CAGACGCTGC AGCCACAGAA CAGAGACGCC TTCTTCAAGA CTCTGGCCAA TCTGGGCATC 
CTGCCGGCAC TGGAGATCGT GATGGGCATG GATGATGTGC AGGTGAAGGC CGCCGCCACA 
GATATCTTCT CCTACCTGGT GGAGTTCAGT CCATCGATGG TGCGTGAGTT CGTGATGCAG 
GAGCCGCAGC AGGCTGATGA TGACGTGCTG CTGATTAACG TGGTGATCAA GCAGATGATC 
TGTGATTCAG ACCCTGAGCT GGGCGGAGCC GTGCAGCTGA TGGGGCTCCT GCGCACACTC 
ATAGACCCCG AGAACATGCT CGCACCCTCC AACAAAACAG AGAAGAGTGA GTTTCTGAGC 
TTCTTCTACA AGTACTGTAT GCATGTGCTG ACGGCTCCAC TGCTGGCCAA CACCGCCGAG 
GAGGACCAAG ATTTGGATGA AGGATCAGCT AAAGTCAACC CTGTGTGTCC AGATAACTTC 
CAGACGGCGC AGCTGCTGGC GCTGATCCTG GAGCTGCTGA CCTTCTGTGT GGAGCATCAC 
ACGTACCACA TCAAGAACTA CATCATGACC AGAGACCTGC TGCGGAGGGT GCTGACGCTC 
ATGAACTCCA AACACACCTT CCTGGCGCTC TGTGCGCTGC GCTTCATGCG GAGGATCATC 
GGCCTGAAGG ATGAGTATTA TAACCGCTAC ATCATGAAGG GGAACCTGTT TGAGCCGGTC 
ATCAACGCTC TGCTGGACAA CGGCACGCGC TACAACCTGC TGAACTCCGC CATCATCGAG 
CTGTTCGACT TCATACGCGT GGAGGACATC CGCTCGCTCA CTGCACACAT TGTGGAGAAC 
TTCCAGAGCG CGCTGCAGTC CATCCAGTAT GTGCAGACCT TCAAGGGCCT GAAGGGGAGA 
TACGAGCAGG AGCAGGAGCG CCAGAGCCGG GGCCTCAGCA GGTTCCGCAG GGACGCGCGC 
TCCTTCCCGG AGGATGAAGA GCTGTGGAAG GAGGAAGAGG AGGATGAGGA GGAGCCAGCG 
GACACGGAGA CGCAACACTG CAAGAGCGAC TACGGGAGAT TCATGGAGAG CGAGAAAGCG 
AAAGAGAACC AGGAGAAGGA GAACCAGGCC AAGCGGAGCA GCAGCGGCAG CTTCAAGTTC 
ACCTTCTCCC ACTCAGCAGC GTCTGCTAAC AGCGCTAAAG CTACATCTGC GTCATCCAGC 
GGAGCCAGCA CTGCAGCCAG GACGGCGCTG GTGGATTACC CAGATGATGA AGATGAGGAT 
GAGGATGATG ATGAAGATCA GCAGGCTCCA CACAAGCGCC CCCGTCTGGG CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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ppp4r3b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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