grm2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	grm2a
Entrez Gene ID	336153
Full Name	glutamate receptor, metabotropic 2a
Synonyms	wu:fj52c07
General protein information	Preferred Names glutamate receptor, metabotropic 2a Names metabotropic glutamate receptor 2 metabotropic glutamate receptor 2a mglur2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166117.4	XP_005166174.1	metabotropic glutamate receptor 2 isoform X1
XM_005166117.3	XP_005166174.1	metabotropic glutamate receptor 2 isoform X1
NM_001302225.1	NP_001289154.1	metabotropic glutamate receptor 2 precursor
XM_021476790.1	XP_021332465.1	metabotropic glutamate receptor 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GRM2	XP_541867.2
Xenopus tropicalis (tropical clawed frog)	grm2	XP_004914127.1
Rattus norvegicus (Norway rat)	Grm2	NP_001099181.1
Mus musculus (house mouse)	Grm2	NP_001153825.1
Gallus gallus (chicken)	GRM2	XP_004944551.1
Homo sapiens (human)	GRM2	NP_000830.2
	GRM2	XP_001095565.1
Bos taurus (cattle)	GRM2	NP_001192795.1
Danio rerio (zebrafish)	grm2a	XP_002662553.3
Danio rerio (zebrafish)	grm7	XP_005172969.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Rab32 and Rab38 genes in chordate pigmentation: an evolutionary perspective.
Coppola U, Annona G, D'Aniello S, Ristoratore F
BMC evolutionary biology1626(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogeny and expression divergence of metabotropic glutamate receptor genes in the brain of zebrafish (Danio rerio).
Haug MF, Gesemann M, Mueller T, Neuhauss SC
The Journal of comparative neurology521(7)1533-60(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following grm2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grm2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40062	NM_001302225.1 Latest version!	Danio rerio glutamate receptor, metabotropic 2a (grm2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa12155	XM_005166117.4 Latest version!	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa12155	XM_021476790.1 Latest version!	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa12155	XM_005166117.3	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grm2a ( NM_001302225.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001289154.1 Danio rerio glutamate receptor, metabotropic 2a (grm2a), mRNA.

Price & Availability↑

CloneID	ODa40062
Clone ID Related Accession (Same CDS sequence)	NM_001302225.1
Accession Version	NM_001302225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	metabotropic glutamate receptor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KF234083.1. ##Evidence-Data-START## Transcript exon combination :: KF234083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476784 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGA GGTCAGCTCA TTTTAGAGGT CACGGGCCCT CCTGGCCGCT TCACCTCACT 
CTGCTGCTGC TTCTGTTGGC CAGGACACCT CAAGCATTGG CAATTCCAGG GTACAACACT 
GACACCTCCA AGAAGGAGAT CGTAATGGAT GGAGATCTAG TGATCGGAGG CCTGTTCCCT 
GTTCATCATA AAGGGGAAGG AGCTCAGGAC TGTGGCCGAA TTAATACACA GAGAGGGATT 
CAGAGACTTG AGGCAATGCT GTTAGCTTTG GACAAGATCA ATCAGGATGA TCAGATTTTG 
CCCGGGGTGA CGTTAGGTGC CCACATTCTG GACACTTGCT CCAAAGACAC ATACGCACTC 
GAGCAGTCAC TTGAATTTGT GCGTGCTTCG CTTACGAAAG TGGACGATAG TGAGTACACG 
TGTCCGGATG GATCCTACGC CATCCACGAT GACGTTCCGC TCGCAATATC AGGCGTGATT 
GGAGGATCCT ACAGCGATGT ATCCATACAG GTGGCCAATC TCCTACGCCT TTTCCAGATC 
CCTCAGATCA GTTATGCCTC CACCAGTGCA AAACTGAGTG ACAAATCCCG CTATGATTAC 
TTTGCCCGGA CTGTACCTCC TGACTTCTAC CAAGCCAAAG CCATGGCTGA GATCCTACGC 
TTCTTCAACT GGACGTACGT CTCCACTGTG GCCTCAGAGG GAGATTACGG CGAGACGGGC 
ATCGACGCCT TTCAGCAAGA AGCTCGGGCC TTACAAATCT GCATTGCAAC ATCTGCCAAA 
GTCAGTCGCT CCATGGACCA ATACAGTTAT GATGGAGTGA TCAGATCTCT GTTGCAGAAT 
TCCAACGCTA AAGTGGTAAT CCTGTTCACC AGAAGGGAAG ATGCAAGAGA GTTGTTGGTC 
GCTGCGAAGC GAATGAATGT CTCCTTCATC TGGGTGGCCA GTGATGGATG GGGAGCGCAG 
GAGGTTGTGG TGAGAGGGAG TGAAAGTGTT GCGGATGGAG CTTTTACTAT TGAGCTTGCG 
TCATATACCA TCCCTGAATT CGCAGCGTAC TTTACAAACC TCAATCCGTA TAACAACACA 
CGTAACCCGT GGTTCAGAGA GTTCTGGGAG AACAAATTCC AGTGCAGTCT GCATGATATT 
ACCTGTGGGA AACATTCTCT TCGGGATGGT AAATTCGAGC AGGAGTCTAA AATCATGTTT 
GTGGTGAATG CAGTGTATGC TATGGCCAAT GCGCTTCACC ACATGAGGCA GGCATTGTGC 
GCCAATAGCA CCAAACTTTG CAGTGCCATG AAGCCTGTGA ACGGGAAAAG GCTGTATAAG 
GACTACATCC TGAAGACAAA GTTTGATGCT CCATTTCGCC CGGCCGATAC TGATAACATC 
ATTCACTTTG ATGCATACGG AGACAGTTTG GGCCGCTACA ACATCTTCCA TTATCACAAA 
GAGAATGATG AGTACGTCTA TAGAAAGGTG GGCTACTGGG CTCAGGATTT GACTCTGAAC 
ACAACCTTAA TTCCTTGGGA GAGCCAAGCG GTGCCCACAT CCCAGTGCAG CGACCCATGT 
AGGAAAAACG AAATTAAAAG CATGCAGCCC GGAGATGTTT GCTGCTGGAT TTGCATTCCC 
TGTCAGCCCT ACCAATACCT CCTGGATGAA TTCACATGTG CGGATTGTAG CTTCGGACAG 
TGGCCTCTAG ACAATCTCAC AGGCTGTTAT GACTTACCTG AGGAGTACAT TCATTGGGAG 
GATGCCTGGG CAATTGGACC TGTCTCTATT GCTTGCCTCG GCATGCTTTG CACGCTCTTC 
GTCATTGGCC TCTTCCTGAA AAACAACGAG ACTCCGGTGG TGAAGGCTAG TGGACGGGAA 
CTTTCCTATA TTCTTCTCCT AGGCGTCCTT CTTTGCTATT TCATGACTTT CATCTACATC 
AGCAAACCTT CAGCCGTTGT GTGCACGTTA CGACGGCTCG GTCTGGGCAC GTCCTTCGCC 
GTTTGCTACT CTGCATTACT CACCAAGACC AATCGCATTG CTCGGATTTT CAACGGTGTT 
AAAGATGGAG CTCAAAGGCC GAGGTTCATC AGTCCAGCCT CACAGGTGGC CATCTGCGCT 
GCTCTTATCT CCTGTCAGCT TCTTGTGGTA GTGGTCTGGC TCCTGGTCGA GGCTCCGGGG 
GTGAGAAAGG AGGTCAGTCC TGAAAGAAGA GACGTGGTGA CATTGAAGTG CAACAGCAAG 
GACTCGAGCA TGCTGATGTC CTTAACCTAC AACTGTGTCC TCATCATCCT CTGTACCTTT 
TATGCCTTTA AGACTCGAAA GTGCCCAGAG AACTTCAACG AAGCCAAGTT CATCGGCTTC 
ACCATGTACA CCACATGTAT AATCTGGCTG GCTTTCCAGC CCATCTTCTA TGTTACTGCC 
AGTGACTATA GGGTACAGAC CACAACCATG TGCATCTCTG TGAGTCTGAG CGGTTCGGTG 
GTTCTGGGCT GCCTCTTCGC TCCCAAAATC CACATCATCC TCTTTCAGCC GCAAAAGAAC 
GTCACCACAC TCAGAGTGGC CACCACGCGC TTTAGTGTGA CCACCGGCCC CGGCTCCAGC 
TTCTCTCAAG CCTCGGCCTC CAACATTGTG CCTACAGTAT GTAATGGCAG AGAGGTAGTG 
GACTCAACAA CATCTTCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289154.1
CDS	3..2666
Translation	MAQRSAHFRGHGPSWPLHLTLLLLLLARTPQALAIPGYNTDTSKKEIVMDGDLVIGGLFPVHHKGEGAQDCGRINTQRGIQRLEAMLLALDKINQDDQILPGVTLGAHILDTCSKDTYALEQSLEFVRASLTKVDDSEYTCPDGSYAIHDDVPLAISGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIDAFQQEARALQICIATSAKVSRSMDQYSYDGVIRSLLQNSNAKVVILFTRREDARELLVAAKRMNVSFIWVASDGWGAQEVVVRGSESVADGAFTIELASYTIPEFAAYFTNLNPYNNTRNPWFREFWENKFQCSLHDITCGKHSLRDGKFEQESKIMFVVNAVYAMANALHHMRQALCANSTKLCSAMKPVNGKRLYKDYILKTKFDAPFRPADTDNIIHFDAYGDSLGRYNIFHYHKENDEYVYRKVGYWAQDLTLNTTLIPWESQAVPTSQCSDPCRKNEIKSMQPGDVCCWICIPCQPYQYLLDEFTCADCSFGQWPLDNLTGCYDLPEEYIHWEDAWAIGPVSIACLGMLCTLFVIGLFLKNNETPVVKASGRELSYILLLGVLLCYFMTFIYISKPSAVVCTLRRLGLGTSFAVCYSALLTKTNRIARIFNGVKDGAQRPRFISPASQVAICAALISCQLLVVVVWLLVEAPGVRKEVSPERRDVVTLKCNSKDSSMLMSLTYNCVLIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIILFQPQKNVTTLRVATTRFSVTTGPGSSFSQASASNIVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	NM_001302225.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, metabotropic 2a (grm2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302225.1

ORF Insert Sequence:

ATGGCTCAGA GGTCAGCTCA TTTTAGAGGT CACGGGCCCT CCTGGCCGCT TCACCTCACT 
CTGCTGCTGC TTCTGTTGGC CAGGACACCT CAAGCATTGG CAATTCCAGG GTACAACACT 
GACACCTCCA AGAAGGAGAT CGTAATGGAT GGAGATCTAG TGATCGGAGG CCTGTTCCCT 
GTTCATCATA AAGGGGAAGG AGCTCAGGAC TGTGGCCGAA TTAATACACA GAGAGGGATT 
CAGAGACTTG AGGCAATGCT GTTAGCTTTG GACAAGATCA ATCAGGATGA TCAGATTTTG 
CCCGGGGTGA CGTTAGGTGC CCACATTCTG GACACTTGCT CCAAAGACAC ATACGCACTC 
GAGCAGTCAC TTGAATTTGT GCGTGCTTCG CTTACGAAAG TGGACGATAG TGAGTACACG 
TGTCCGGATG GATCCTACGC CATCCACGAT GACGTTCCGC TCGCAATATC AGGCGTGATT 
GGAGGATCCT ACAGCGATGT ATCCATACAG GTGGCCAATC TCCTACGCCT TTTCCAGATC 
CCTCAGATCA GTTATGCCTC CACCAGTGCA AAACTGAGTG ACAAATCCCG CTATGATTAC 
TTTGCCCGGA CTGTACCTCC TGACTTCTAC CAAGCCAAAG CCATGGCTGA GATCCTACGC 
TTCTTCAACT GGACGTACGT CTCCACTGTG GCCTCAGAGG GAGATTACGG CGAGACGGGC 
ATCGACGCCT TTCAGCAAGA AGCTCGGGCC TTACAAATCT GCATTGCAAC ATCTGCCAAA 
GTCAGTCGCT CCATGGACCA ATACAGTTAT GATGGAGTGA TCAGATCTCT GTTGCAGAAT 
TCCAACGCTA AAGTGGTAAT CCTGTTCACC AGAAGGGAAG ATGCAAGAGA GTTGTTGGTC 
GCTGCGAAGC GAATGAATGT CTCCTTCATC TGGGTGGCCA GTGATGGATG GGGAGCGCAG 
GAGGTTGTGG TGAGAGGGAG TGAAAGTGTT GCGGATGGAG CTTTTACTAT TGAGCTTGCG 
TCATATACCA TCCCTGAATT CGCAGCGTAC TTTACAAACC TCAATCCGTA TAACAACACA 
CGTAACCCGT GGTTCAGAGA GTTCTGGGAG AACAAATTCC AGTGCAGTCT GCATGATATT 
ACCTGTGGGA AACATTCTCT TCGGGATGGT AAATTCGAGC AGGAGTCTAA AATCATGTTT 
GTGGTGAATG CAGTGTATGC TATGGCCAAT GCGCTTCACC ACATGAGGCA GGCATTGTGC 
GCCAATAGCA CCAAACTTTG CAGTGCCATG AAGCCTGTGA ACGGGAAAAG GCTGTATAAG 
GACTACATCC TGAAGACAAA GTTTGATGCT CCATTTCGCC CGGCCGATAC TGATAACATC 
ATTCACTTTG ATGCATACGG AGACAGTTTG GGCCGCTACA ACATCTTCCA TTATCACAAA 
GAGAATGATG AGTACGTCTA TAGAAAGGTG GGCTACTGGG CTCAGGATTT GACTCTGAAC 
ACAACCTTAA TTCCTTGGGA GAGCCAAGCG GTGCCCACAT CCCAGTGCAG CGACCCATGT 
AGGAAAAACG AAATTAAAAG CATGCAGCCC GGAGATGTTT GCTGCTGGAT TTGCATTCCC 
TGTCAGCCCT ACCAATACCT CCTGGATGAA TTCACATGTG CGGATTGTAG CTTCGGACAG 
TGGCCTCTAG ACAATCTCAC AGGCTGTTAT GACTTACCTG AGGAGTACAT TCATTGGGAG 
GATGCCTGGG CAATTGGACC TGTCTCTATT GCTTGCCTCG GCATGCTTTG CACGCTCTTC 
GTCATTGGCC TCTTCCTGAA AAACAACGAG ACTCCGGTGG TGAAGGCTAG TGGACGGGAA 
CTTTCCTATA TTCTTCTCCT AGGCGTCCTT CTTTGCTATT TCATGACTTT CATCTACATC 
AGCAAACCTT CAGCCGTTGT GTGCACGTTA CGACGGCTCG GTCTGGGCAC GTCCTTCGCC 
GTTTGCTACT CTGCATTACT CACCAAGACC AATCGCATTG CTCGGATTTT CAACGGTGTT 
AAAGATGGAG CTCAAAGGCC GAGGTTCATC AGTCCAGCCT CACAGGTGGC CATCTGCGCT 
GCTCTTATCT CCTGTCAGCT TCTTGTGGTA GTGGTCTGGC TCCTGGTCGA GGCTCCGGGG 
GTGAGAAAGG AGGTCAGTCC TGAAAGAAGA GACGTGGTGA CATTGAAGTG CAACAGCAAG 
GACTCGAGCA TGCTGATGTC CTTAACCTAC AACTGTGTCC TCATCATCCT CTGTACCTTT 
TATGCCTTTA AGACTCGAAA GTGCCCAGAG AACTTCAACG AAGCCAAGTT CATCGGCTTC 
ACCATGTACA CCACATGTAT AATCTGGCTG GCTTTCCAGC CCATCTTCTA TGTTACTGCC 
AGTGACTATA GGGTACAGAC CACAACCATG TGCATCTCTG TGAGTCTGAG CGGTTCGGTG 
GTTCTGGGCT GCCTCTTCGC TCCCAAAATC CACATCATCC TCTTTCAGCC GCAAAAGAAC 
GTCACCACAC TCAGAGTGGC CACCACGCGC TTTAGTGTGA CCACCGGCCC CGGCTCCAGC 
TTCTCTCAAG CCTCGGCCTC CAACATTGTG CCTACAGTAT GTAATGGCAG AGAGGTAGTG 
GACTCAACAA CATCTTCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm2a ( XM_005166117.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166174.1 Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12155
Clone ID Related Accession (Same CDS sequence)	XM_005166117.4 , XM_005166117.3 , XM_021476790.1
Accession Version	XM_005166117.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	metabotropic glutamate receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166117.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166174.1
CDS	858..3542
Translation	MHRSLRTMAQRSAHFRGHGPSWPLHLTLLLLLLARTPQALAIPGYNTDTSKKEIVMDGDLVIGGLFPVHHKGEGAQDCGRINTQRGIQRLEAMLLALDKINQDDQILPGVTLGAHILDTCSKDTYALEQSLEFVRASLTKVDDSEYTCPDGSYAIHDDVPLAISGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIDAFQQEARALQICIATSAKVSRSMDQYSYDGVIRSLLQKSNAKVVILFTRSEDARELLVAAKRMNVSFIWVASDGWGAQEVVVRGSESVADGAFTIELASYTIPEFAAYFTNLNPYNNTRNPWFREFWENKFQCSLHDITCGKHSLRDGKFEQESKIMFVVNAVYAMANALHHMRQALCANSTKLCSAMKPVNGKRLYKDYILKTKFDAPFRPADTDNIIHFDAYGDSLGRYNIFHYHKENDEYVYRKVGYWAQDLTLNTTLIPWESQAVPTSQCSDPCRKNEIKSMQPGDVCCWICIPCQPYQYLLDEFTCADCSFGQWPLDNLTGCYDLPEEYIHWEDAWAIGPVSIACLGMLCTLFVIGLFLKNNETPVVKASGRELSYILLLGVLLCYFMTFIYISKPSAVVCTLRRLGLGTSFAVCYSALLTKTNRIARIFNGVKDGAQRPRFISPASQVAICAALISCQLLVVVVWLLVEAPGVRKEVSPERRDVVTLKCNSKDSSMLMSLTYNCVLIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIILFQPQKNVTTLRVATTRFSVTTGPGSSFSQASASNIVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	XM_005166117.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166117.4

ORF Insert Sequence:

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm2a ( XM_005166117.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166174.1 Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12155
Clone ID Related Accession (Same CDS sequence)	XM_005166117.4 , XM_005166117.3 , XM_021476790.1
Accession Version	XM_005166117.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	metabotropic glutamate receptor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166174.1
CDS	841..3525
Translation	MHRSLRTMAQRSAHFRGHGPSWPLHLTLLLLLLARTPQALAIPGYNTDTSKKEIVMDGDLVIGGLFPVHHKGEGAQDCGRINTQRGIQRLEAMLLALDKINQDDQILPGVTLGAHILDTCSKDTYALEQSLEFVRASLTKVDDSEYTCPDGSYAIHDDVPLAISGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIDAFQQEARALQICIATSAKVSRSMDQYSYDGVIRSLLQKSNAKVVILFTRSEDARELLVAAKRMNVSFIWVASDGWGAQEVVVRGSESVADGAFTIELASYTIPEFAAYFTNLNPYNNTRNPWFREFWENKFQCSLHDITCGKHSLRDGKFEQESKIMFVVNAVYAMANALHHMRQALCANSTKLCSAMKPVNGKRLYKDYILKTKFDAPFRPADTDNIIHFDAYGDSLGRYNIFHYHKENDEYVYRKVGYWAQDLTLNTTLIPWESQAVPTSQCSDPCRKNEIKSMQPGDVCCWICIPCQPYQYLLDEFTCADCSFGQWPLDNLTGCYDLPEEYIHWEDAWAIGPVSIACLGMLCTLFVIGLFLKNNETPVVKASGRELSYILLLGVLLCYFMTFIYISKPSAVVCTLRRLGLGTSFAVCYSALLTKTNRIARIFNGVKDGAQRPRFISPASQVAICAALISCQLLVVVVWLLVEAPGVRKEVSPERRDVVTLKCNSKDSSMLMSLTYNCVLIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIILFQPQKNVTTLRVATTRFSVTTGPGSSFSQASASNIVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	XM_005166117.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166117.3

ORF Insert Sequence:

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm2a ( XM_021476790.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332465.1 Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa12155
Clone ID Related Accession (Same CDS sequence)	XM_005166117.4 , XM_005166117.3 , XM_021476790.1
Accession Version	XM_021476790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	metabotropic glutamate receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332465.1
CDS	853..3537
Translation	MHRSLRTMAQRSAHFRGHGPSWPLHLTLLLLLLARTPQALAIPGYNTDTSKKEIVMDGDLVIGGLFPVHHKGEGAQDCGRINTQRGIQRLEAMLLALDKINQDDQILPGVTLGAHILDTCSKDTYALEQSLEFVRASLTKVDDSEYTCPDGSYAIHDDVPLAISGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIDAFQQEARALQICIATSAKVSRSMDQYSYDGVIRSLLQKSNAKVVILFTRSEDARELLVAAKRMNVSFIWVASDGWGAQEVVVRGSESVADGAFTIELASYTIPEFAAYFTNLNPYNNTRNPWFREFWENKFQCSLHDITCGKHSLRDGKFEQESKIMFVVNAVYAMANALHHMRQALCANSTKLCSAMKPVNGKRLYKDYILKTKFDAPFRPADTDNIIHFDAYGDSLGRYNIFHYHKENDEYVYRKVGYWAQDLTLNTTLIPWESQAVPTSQCSDPCRKNEIKSMQPGDVCCWICIPCQPYQYLLDEFTCADCSFGQWPLDNLTGCYDLPEEYIHWEDAWAIGPVSIACLGMLCTLFVIGLFLKNNETPVVKASGRELSYILLLGVLLCYFMTFIYISKPSAVVCTLRRLGLGTSFAVCYSALLTKTNRIARIFNGVKDGAQRPRFISPASQVAICAALISCQLLVVVVWLLVEAPGVRKEVSPERRDVVTLKCNSKDSSMLMSLTYNCVLIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIILFQPQKNVTTLRVATTRFSVTTGPGSSFSQASASNIVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	XM_021476790.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, metabotropic 2a (grm2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476790.1

ORF Insert Sequence:

ATGCACCGAT CTTTGCGCAC CATGGCTCAG AGGTCAGCTC ATTTTAGAGG TCACGGGCCC 
TCCTGGCCGC TTCACCTCAC TCTGCTGCTG CTTCTGTTGG CCAGGACACC TCAAGCATTG 
GCAATTCCAG GGTACAACAC TGACACCTCC AAGAAGGAGA TCGTAATGGA TGGAGATCTA 
GTGATCGGAG GCCTGTTCCC TGTTCATCAT AAAGGGGAAG GAGCTCAGGA CTGTGGCCGA 
ATTAATACAC AGAGAGGGAT TCAGAGACTT GAGGCAATGC TGTTAGCTTT GGACAAGATC 
AATCAGGATG ATCAGATTTT GCCCGGGGTG ACGTTAGGTG CCCACATTCT GGACACTTGC 
TCCAAAGACA CATACGCACT CGAGCAGTCA CTTGAATTTG TGCGTGCTTC GCTTACGAAA 
GTGGACGATA GTGAGTACAC GTGTCCGGAT GGATCCTACG CCATCCACGA TGACGTTCCG 
CTCGCAATAT CAGGCGTGAT TGGAGGATCC TACAGCGATG TATCCATACA GGTGGCCAAT 
CTCCTACGCC TTTTCCAGAT CCCTCAGATC AGTTATGCCT CCACCAGTGC AAAACTGAGT 
GACAAATCCC GCTATGATTA CTTTGCCCGG ACTGTACCTC CTGACTTCTA CCAAGCCAAA 
GCCATGGCTG AGATCCTACG CTTCTTCAAC TGGACGTACG TCTCCACTGT GGCCTCAGAG 
GGAGATTACG GCGAGACGGG CATCGACGCC TTTCAGCAAG AAGCTCGGGC CTTACAAATC 
TGCATTGCAA CATCTGCCAA AGTCAGTCGC TCCATGGACC AATACAGTTA TGATGGAGTG 
ATCAGATCTC TGTTGCAGAA GTCCAACGCT AAAGTGGTAA TCCTGTTCAC CAGAAGTGAA 
GATGCAAGAG AGTTGTTGGT CGCTGCGAAG CGAATGAATG TCTCCTTCAT CTGGGTGGCC 
AGTGATGGAT GGGGAGCGCA GGAGGTTGTG GTGAGAGGGA GTGAAAGTGT TGCGGATGGA 
GCTTTTACTA TTGAGCTTGC GTCATATACC ATCCCTGAAT TCGCAGCGTA CTTTACAAAC 
CTCAATCCGT ATAACAACAC ACGTAACCCG TGGTTCAGAG AGTTCTGGGA GAACAAATTC 
CAGTGCAGTC TGCATGATAT TACCTGTGGG AAACATTCTC TTCGGGATGG TAAATTCGAG 
CAGGAGTCTA AAATCATGTT TGTGGTGAAT GCAGTGTATG CTATGGCCAA TGCGCTTCAC 
CACATGAGGC AGGCATTGTG CGCCAATAGC ACCAAACTTT GCAGTGCCAT GAAACCTGTG 
AACGGGAAAA GGCTGTATAA GGACTACATC CTGAAGACAA AGTTTGATGC TCCATTTCGC 
CCGGCCGATA CTGATAACAT CATTCACTTT GATGCATACG GAGACAGTTT GGGCCGCTAC 
AACATCTTCC ATTATCACAA AGAGAATGAT GAGTACGTCT ATAGAAAGGT GGGCTACTGG 
GCTCAGGATT TGACTCTGAA CACAACCTTA ATTCCTTGGG AGAGCCAAGC GGTGCCCACA 
TCCCAGTGCA GCGACCCATG TAGGAAAAAC GAAATTAAAA GCATGCAGCC CGGAGATGTT 
TGCTGCTGGA TTTGCATTCC CTGTCAGCCC TACCAATACC TCCTGGATGA ATTCACATGT 
GCGGATTGTA GCTTCGGACA GTGGCCTCTA GACAATCTCA CAGGCTGTTA TGACTTACCT 
GAGGAGTACA TTCATTGGGA GGATGCCTGG GCAATTGGAC CTGTCTCTAT TGCTTGCCTC 
GGCATGCTTT GCACGCTCTT CGTCATTGGC CTCTTCCTGA AAAACAACGA GACTCCGGTG 
GTGAAGGCTA GTGGACGGGA ACTTTCCTAT ATTCTTCTCC TAGGCGTCCT TCTTTGCTAT 
TTCATGACTT TCATCTACAT CAGCAAACCT TCAGCCGTTG TGTGCACGTT ACGACGGCTC 
GGTCTGGGCA CGTCCTTCGC CGTTTGCTAC TCTGCATTAC TCACCAAGAC CAATCGCATT 
GCTCGGATTT TCAACGGTGT TAAAGATGGA GCTCAAAGGC CGAGGTTCAT CAGTCCAGCC 
TCACAGGTGG CCATCTGCGC TGCTCTTATC TCCTGTCAGC TTCTTGTGGT AGTGGTCTGG 
CTCCTGGTCG AGGCTCCGGG GGTGAGAAAG GAGGTCAGTC CTGAAAGAAG AGACGTGGTG 
ACATTGAAGT GCAACAGCAA GGACTCGAGC ATGCTGATGT CCTTAACCTA CAACTGTGTC 
CTCATCATCC TCTGTACCTT TTATGCCTTT AAGACTCGAA AGTGCCCAGA GAACTTCAAC 
GAAGCCAAGT TCATCGGCTT CACCATGTAC ACCACATGTA TAATCTGGCT GGCTTTCCAG 
CCCATCTTCT ATGTTACTGC CAGTGACTAT AGGGTACAGA CCACAACCAT GTGCATCTCT 
GTGAGTCTGA GCGGTTCGGT GGTTCTGGGC TGCCTCTTCG CTCCCAAAAT CCACATCATC 
CTCTTTCAGC CGCAAAAGAA CGTCACCACA CTCAGAGTGG CCACCACGCG CTTTAGTGTG 
ACCACCGGCC CCGGCTCCAG CTTCTCTCAA GCCTCGGCCT CCAACATTGT GCCTACAGTA 
TGTAATGGCA GAGAGGTAGT GGACTCAACA ACATCTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Rab32 and Rab38 genes in chordate pigmentation: an evolutionary perspective.
BMC evolutionary biology1626(2016 Jan)
Coppola U,Annona G,D'Aniello S,Ristoratore F

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogeny and expression divergence of metabotropic glutamate receptor genes in the brain of zebrafish (Danio rerio).
The Journal of comparative neurology521(7)1533-60(2013 May)
Haug MF,Gesemann M,Mueller T,Neuhauss SC

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grm2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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