bnip3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bnip3
Entrez Gene ID	336116
Full Name	BCL2 interacting protein 3
Synonyms	fj49c01,nip3c,wu:fj49c01,zgc:198406
General protein information	Preferred Names BCL2 interacting protein 3 Names BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 BCL2/adenovirus E1B interacting protein 3 Bcl-2/adenovirus E1B 19kD interaction protein 3C
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172968.3	XP_005173025.1	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 isoform X1
XM_005172968.4	XP_005173025.1	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 isoform X1
NM_001012245.1	NP_001012245.1	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC607408	XP_849147.4
Pan troglodytes (chimpanzee)	BNIP3	XP_508116.2
Bos taurus (cattle)	BNIP3	NP_001069834.1
Rattus norvegicus (Norway rat)	Bnip3	NP_445872.1
Danio rerio (zebrafish)	bnip3	NP_001012245.1
Mus musculus (house mouse)	Bnip3	NP_033890.1
Gallus gallus (chicken)	BNIP3	XP_421829.3
Homo sapiens (human)	BNIP3	NP_004043.2
Xenopus tropicalis (tropical clawed frog)	bnip3	NP_001015859.1

Gene Ontology
Bibliography

Related articles in PubMed

The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erb? and indirectly via Cebpb/(C/ebp?) in zebrafish.
Huang G, Zhang F, Ye Q, Wang H
Autophagy12(8)1292-309(2016 Aug)

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Garcia-Reyero N, Escalon L, Prats E, Faria M, Soares AM, Rald?a D
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)

p53 directly suppresses BNIP3 expression to protect against hypoxia-induced cell death.
Feng X, Liu X, Zhang W, Xiao W
The EMBO journal30(16)3397-415(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish nip3a was induced by hypoxia. p53 mutation/knockdown enhanced nip3a expression under hypoxia resulted in cell death enhancement in p53 mutant embryos.
Title: p53 directly suppresses BNIP3 expression to protect against hypoxia-induced cell death.

The following bnip3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bnip3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36564	NM_001012245.1 Latest version!	Danio rerio BCL2 interacting protein 3 (bnip3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa18174	XM_005172968.4 Latest version!	Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa18174	XM_005172968.3	Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bnip3 ( NM_001012245.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001012245.1 Danio rerio BCL2 interacting protein 3 (bnip3), mRNA.

Price & Availability↑

CloneID	ODa36564
Clone ID Related Accession (Same CDS sequence)	NM_001012245.1
Accession Version	NM_001012245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Danio rerio(zebrafish)
Product	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY424950.1. On Jul 6, 2005 this sequence version replaced XM_687959.1. ##Evidence-Data-START## Transcript exon combination :: AY424950.1, BC171679.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTACAA GATCCCAGAC TCCTTTGCAC 
TTGCGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATGTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012245.1
CDS	76..627
Translation	MSIEKHSVSEENLQGSWVELHFNNGGGSTSKAATDEQSASTAPSGDLEKMLLDAQHESGRSSSRGSLPCDSPTRSQTPLHLRRGSEVHSSGEKNSSQSEEDYVERRKEVEILMKKNADWIWDWSSRPENLPPKEFLLRHPKRSSTLSMRNTSVMKKGGIFSAEFLKVFLPSLVLSHILAVGLG

Target ORF information:

RefSeq Version	NM_001012245.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BCL2 interacting protein 3 (bnip3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012245.1

ORF Insert Sequence:

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTACAA GATCCCAGAC TCCTTTGCAC 
TTGCGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATGTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bnip3 ( XM_005172968.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173025.1 Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18174
Clone ID Related Accession (Same CDS sequence)	XM_005172968.3 , XM_005172968.4
Accession Version	XM_005172968.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTCCAA GATCCCAGAC TCCTTTGCAC 
TTGTGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATTTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGG TGTACATCGG AAGGCGTTTT ACTGCTTCTG GCACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173025.1
CDS	354..944
Translation	MSIEKHSVSEENLQGSWVELHFNNGGGSTSKAATDEQSASTAPSGDLEKMLLDAQHESGRSSSRGSLPCDSPPRSQTPLHLCRGSEVHSSGEKNSSQSEEDYLERRKEVEILMKKNADWIWDWSSRPENLPPKEFLLRHPKRSSTLSMRNTSVMKKGGIFSAEFLKVFLPSLVLSHILAVGLGVYIGRRFTASGTF

Target ORF information:

RefSeq Version	XM_005172968.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172968.3

ORF Insert Sequence:

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTCCAA GATCCCAGAC TCCTTTGCAC 
TTGTGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATTTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGG TGTACATCGG AAGGCGTTTT ACTGCTTCTG GCACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bnip3 ( XM_005172968.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173025.1 Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18174
Clone ID Related Accession (Same CDS sequence)	XM_005172968.3 , XM_005172968.4
Accession Version	XM_005172968.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172968.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTCCAA GATCCCAGAC TCCTTTGCAC 
TTGTGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATTTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGG TGTACATCGG AAGGCGTTTT ACTGCTTCTG GCACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173025.1
CDS	349..939
Translation	MSIEKHSVSEENLQGSWVELHFNNGGGSTSKAATDEQSASTAPSGDLEKMLLDAQHESGRSSSRGSLPCDSPPRSQTPLHLCRGSEVHSSGEKNSSQSEEDYLERRKEVEILMKKNADWIWDWSSRPENLPPKEFLLRHPKRSSTLSMRNTSVMKKGGIFSAEFLKVFLPSLVLSHILAVGLGVYIGRRFTASGTF

Target ORF information:

RefSeq Version	XM_005172968.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BCL2/adenovirus E1B interacting protein 3 (bnip3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172968.4

ORF Insert Sequence:

ATGTCGATTG AAAAGCACAG CGTGTCTGAA GAAAACCTTC AGGGTTCTTG GGTGGAGCTG 
CATTTTAATA ATGGGGGAGG CAGCACTTCT AAAGCAGCCA CAGATGAACA GTCAGCTAGC 
ACGGCCCCGA GCGGAGACCT GGAGAAGATG CTTCTGGATG CTCAGCACGA GTCGGGTCGG 
AGCAGCTCCA GAGGAAGCCT ACCATGTGAC AGTCCTCCAA GATCCCAGAC TCCTTTGCAC 
TTGTGCAGGG GCTCCGAGGT CCACAGCTCT GGGGAAAAAA ACAGCTCACA GTCAGAGGAA 
GACTATTTGG AGAGGAGAAA AGAGGTGGAG ATCCTGATGA AGAAAAATGC TGATTGGATC 
TGGGACTGGT CGAGTCGCCC AGAAAACCTG CCACCCAAGG AGTTTCTGCT GCGGCACCCG 
AAGCGCTCCA GCACACTCAG CATGAGGAAC ACCAGTGTGA TGAAGAAAGG AGGAATCTTC 
TCTGCTGAGT TCCTCAAAGT CTTCCTGCCC TCTCTGGTCC TCTCACACAT CCTCGCTGTG 
GGTCTCGGGG TGTACATCGG AAGGCGTTTT ACTGCTTCTG GCACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erb? and indirectly via Cebpb/(C/ebp?) in zebrafish.
Autophagy12(8)1292-309(2016 Aug)
Huang G,Zhang F,Ye Q,Wang H

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)
Garcia-Reyero N,Escalon L,Prats E,Faria M,Soares AM,Rald?a D

p53 directly suppresses BNIP3 expression to protect against hypoxia-induced cell death.
The EMBO journal30(16)3397-415(2011 Jul)
Feng X,Liu X,Zhang W,Xiao W

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bnip3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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