trim9 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim9
Entrez Gene ID	336099
Full Name	tripartite motif containing 9
Synonyms	fj48c11,wu:fj48c11,zgc:77357
General protein information	Preferred Names tripartite motif containing 9 Names E3 ubiquitin-protein ligase TRIM9
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156984.3	XP_005157041.1	E3 ubiquitin-protein ligase TRIM9 isoform X2
XM_005156986.3	XP_005157043.1	E3 ubiquitin-protein ligase TRIM9 isoform X4
XM_005156983.3	XP_005157040.1	E3 ubiquitin-protein ligase TRIM9 isoform X1
XM_009307466.2	XP_009305741.1	E3 ubiquitin-protein ligase TRIM9 isoform X3
XM_005156984.4	XP_005157041.1	E3 ubiquitin-protein ligase TRIM9 isoform X2
XM_005156986.4	XP_005157043.1	E3 ubiquitin-protein ligase TRIM9 isoform X3
NM_205563.1	NP_991126.1	E3 ubiquitin-protein ligase TRIM9
XM_005156983.4	XP_005157040.1	E3 ubiquitin-protein ligase TRIM9 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Trim9	NP_569104.1
Danio rerio (zebrafish)	trim9	NP_991126.1
Drosophila melanogaster (fruit fly)	Trim9	NP_001188786.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008750	XP_314868.3
Caenorhabditis elegans (roundworm)	trim-9	NP_503395.1
Xenopus tropicalis (tropical clawed frog)	trim9	XP_004917217.1
Canis lupus familiaris (dog)	TRIM9	XP_547809.3
Bos taurus (cattle)	TRIM9	NP_001070005.1
Mus musculus (house mouse)	Trim9	NP_001273317.1
Homo sapiens (human)	TRIM9	NP_055978.4
Pan troglodytes (chimpanzee)	TRIM9	XP_001156684.2
	TRIM9	XP_001102351.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of Trim9 function abrogates macrophage motility in vivo.
Tokarz DA, Heffelfinger AK, Jima DD, Gerlach J, Shah RN, Rodriguez-Nunez I, Kortum AN, Fletcher AA, Nordone SK, Law JM, Heber S, Yoder JA
Journal of leukocyte biology102(6)1371-1380(2017 Dec)

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Loponte S, Segr? CV, Senese S, Miccolo C, Santaguida S, Deflorian G, Citro S, Mattoscio D, Pisati F, Moser MA, Visintin R, Seiser C, Chiocca S
Scientific reports630213(2016 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Novel cardiovascular gene functions revealed via systematic phenotype prediction in zebrafish.
Musso G, Tasan M, Mosimann C, Beaver JE, Plovie E, Carr LA, Chua HN, Dunham J, Zuberi K, Rodriguez H, Morris Q, Zon L, Roth FP, MacRae CA
Development (Cambridge, England)141(1)224-35(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Trim9 mediates cellular movement and migration in macrophages as well as neurons.
Title: Disruption of Trim9 function abrogates macrophage motility in vivo.

The following trim9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03119	NM_205563.1 Latest version!	Danio rerio tripartite motif containing 9 (trim9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18974	XM_005156983.4 Latest version!	Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18975	XM_005156984.4 Latest version!	Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18977	XM_005156986.4 Latest version!	Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa40061	XM_009307466.2 Latest version!	Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa18975	XM_005156984.3	Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18977	XM_005156986.3	Danio rerio tripartite motif containing 9 (trim9), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18974	XM_005156983.3	Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim9 ( NM_205563.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991126.1 Danio rerio tripartite motif containing 9 (trim9), mRNA.

Price & Availability↑

CloneID	ODa03119
Clone ID Related Accession (Same CDS sequence)	NM_205563.1
Accession Version	NM_205563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065940.1. ##Evidence-Data-START## Transcript exon combination :: BC065940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505372, SAMEA4476746 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCAG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATA 
ACTGATGGAG GGATAAGTAA AGGAGCTACG GTGGGTGTTT TGCTGGATTT CACCCGTGGG 
ATTCTCTTGT TTTTGATAAA TGATGAGCAA CAGGGACCCG TTGCCTTCAA TACACTGGAG 
GGCATGTACT ACCCAGCCAT CAGCCTCAAC CGCAATGTTC AGGTGACTCT TCATTCTGGG 
CTGCCTATTC CTGATTTTTA TACACCCGGC GAGTCTGAAG CTGCAGGCTC AGTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991126.1
CDS	97..2196
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSSFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEIAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAITDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	NM_205563.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205563.1

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCAG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATA 
ACTGATGGAG GGATAAGTAA AGGAGCTACG GTGGGTGTTT TGCTGGATTT CACCCGTGGG 
ATTCTCTTGT TTTTGATAAA TGATGAGCAA CAGGGACCCG TTGCCTTCAA TACACTGGAG 
GGCATGTACT ACCCAGCCAT CAGCCTCAAC CGCAATGTTC AGGTGACTCT TCATTCTGGG 
CTGCCTATTC CTGATTTTTA TACACCCGGC GAGTCTGAAG CTGCAGGCTC AGTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156983.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157040.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18974
Clone ID Related Accession (Same CDS sequence)	XM_005156983.3 , XM_005156983.4
Accession Version	XM_005156983.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATGT ATGTGGACAA CAACCGCTCC 
TGGTTCATGC ATAACAACTC ACACACCAAC CGAACTGATG GAGGGATAAG TAAAGGAGCT 
ACGGTGGGTG TTTTGCTGGA TTTCACCCGT GGGATTCTCT TGTTTTTGAT AAATGATGAG 
CAACAGGGAC CCGTTGCCTT CAATACACTG GAGGGCATGT ACTACCCAGC CATCAGCCTC 
AACCGCAATG TTCAGGTGAC TCTTCATTCT GGGCTGCCTA TTCCTGATTT TTATACACCC 
GGCGAGTCTG AAGCTGCAGG CTCAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157040.1
CDS	399..2591
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEMSLPSYGSQTLATVAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156983.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156983.3

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATGT ATGTGGACAA CAACCGCTCC 
TGGTTCATGC ATAACAACTC ACACACCAAC CGAACTGATG GAGGGATAAG TAAAGGAGCT 
ACGGTGGGTG TTTTGCTGGA TTTCACCCGT GGGATTCTCT TGTTTTTGAT AAATGATGAG 
CAACAGGGAC CCGTTGCCTT CAATACACTG GAGGGCATGT ACTACCCAGC CATCAGCCTC 
AACCGCAATG TTCAGGTGAC TCTTCATTCT GGGCTGCCTA TTCCTGATTT TTATACACCC 
GGCGAGTCTG AAGCTGCAGG CTCAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156983.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157040.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18974
Clone ID Related Accession (Same CDS sequence)	XM_005156983.3 , XM_005156983.4
Accession Version	XM_005156983.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156983.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATGT ATGTGGACAA CAACCGCTCC 
TGGTTCATGC ATAACAACTC ACACACCAAC CGAACTGATG GAGGGATAAG TAAAGGAGCT 
ACGGTGGGTG TTTTGCTGGA TTTCACCCGT GGGATTCTCT TGTTTTTGAT AAATGATGAG 
CAACAGGGAC CCGTTGCCTT CAATACACTG GAGGGCATGT ACTACCCAGC CATCAGCCTC 
AACCGCAATG TTCAGGTGAC TCTTCATTCT GGGCTGCCTA TTCCTGATTT TTATACACCC 
GGCGAGTCTG AAGCTGCAGG CTCAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157040.1
CDS	447..2639
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEMSLPSYGSQTLATVAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156983.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156983.4

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATGT ATGTGGACAA CAACCGCTCC 
TGGTTCATGC ATAACAACTC ACACACCAAC CGAACTGATG GAGGGATAAG TAAAGGAGCT 
ACGGTGGGTG TTTTGCTGGA TTTCACCCGT GGGATTCTCT TGTTTTTGAT AAATGATGAG 
CAACAGGGAC CCGTTGCCTT CAATACACTG GAGGGCATGT ACTACCCAGC CATCAGCCTC 
AACCGCAATG TTCAGGTGAC TCTTCATTCT GGGCTGCCTA TTCCTGATTT TTATACACCC 
GGCGAGTCTG AAGCTGCAGG CTCAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156984.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157041.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18975
Clone ID Related Accession (Same CDS sequence)	XM_005156984.3 , XM_005156984.4
Accession Version	XM_005156984.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156984.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATG 
TATGTGGACA ACAACCGCTC CTGGTTCATG CATAACAACT CACACACCAA CCGAACTGAT 
GGAGGGATAA GTAAAGGAGC TACGGTGGGT GTTTTGCTGG ATTTCACCCG TGGGATTCTC 
TTGTTTTTGA TAAATGATGA GCAACAGGGA CCCGTTGCCT TCAATACACT GGAGGGCATG 
TACTACCCAG CCATCAGCCT CAACCGCAAT GTTCAGGTGA CTCTTCATTC TGGGCTGCCT 
ATTCCTGATT TTTATACACC CGGCGAGTCT GAAGCTGCAG GCTCAGTCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157041.1
CDS	396..2549
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEIAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156984.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156984.3

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATG 
TATGTGGACA ACAACCGCTC CTGGTTCATG CATAACAACT CACACACCAA CCGAACTGAT 
GGAGGGATAA GTAAAGGAGC TACGGTGGGT GTTTTGCTGG ATTTCACCCG TGGGATTCTC 
TTGTTTTTGA TAAATGATGA GCAACAGGGA CCCGTTGCCT TCAATACACT GGAGGGCATG 
TACTACCCAG CCATCAGCCT CAACCGCAAT GTTCAGGTGA CTCTTCATTC TGGGCTGCCT 
ATTCCTGATT TTTATACACC CGGCGAGTCT GAAGCTGCAG GCTCAGTCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156984.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157041.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18975
Clone ID Related Accession (Same CDS sequence)	XM_005156984.3 , XM_005156984.4
Accession Version	XM_005156984.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156984.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATG 
TATGTGGACA ACAACCGCTC CTGGTTCATG CATAACAACT CACACACCAA CCGAACTGAT 
GGAGGGATAA GTAAAGGAGC TACGGTGGGT GTTTTGCTGG ATTTCACCCG TGGGATTCTC 
TTGTTTTTGA TAAATGATGA GCAACAGGGA CCCGTTGCCT TCAATACACT GGAGGGCATG 
TACTACCCAG CCATCAGCCT CAACCGCAAT GTTCAGGTGA CTCTTCATTC TGGGCTGCCT 
ATTCCTGATT TTTATACACC CGGCGAGTCT GAAGCTGCAG GCTCAGTCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157041.1
CDS	416..2569
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEIAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156984.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156984.4

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT AGCCTGGTTC 
ACCTTTGACT CCGCATCGGC TCATCCTGAC ATCATCTTGT CCAATGACAA CCTCACGGTG 
ACGTGTAACA GCTACGACGA CCGCGTGGTC ATGGGAAACA CTGGCTTTTC TCGTGGCGTG 
CATTACTGGG AGATGACCAT CGATCGCTAT GATAACCACC CTGACCCAGC GTTTGGCGTC 
GCCCGGGCCG ATGTGATGAA GGATGTGATG CTGGGCAAGG ATGATAAAGC ATGGGCCATG 
TATGTGGACA ACAACCGCTC CTGGTTCATG CATAACAACT CACACACCAA CCGAACTGAT 
GGAGGGATAA GTAAAGGAGC TACGGTGGGT GTTTTGCTGG ATTTCACCCG TGGGATTCTC 
TTGTTTTTGA TAAATGATGA GCAACAGGGA CCCGTTGCCT TCAATACACT GGAGGGCATG 
TACTACCCAG CCATCAGCCT CAACCGCAAT GTTCAGGTGA CTCTTCATTC TGGGCTGCCT 
ATTCCTGATT TTTATACACC CGGCGAGTCT GAAGCTGCAG GCTCAGTCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156986.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157043.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa18977
Clone ID Related Accession (Same CDS sequence)	XM_005156986.3 , XM_005156986.4
Accession Version	XM_005156986.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATAA CTGATGGAGG GATAAGTAAA 
GGAGCTACGG TGGGTGTTTT GCTGGATTTC ACCCGTGGGA TTCTCTTGTT TTTGATAAAT 
GATGAGCAAC AGGGACCCGT TGCCTTCAAT ACACTGGAGG GCATGTACTA CCCAGCCATC 
AGCCTCAACC GCAATGTTCA GGTGACTCTT CATTCTGGGC TGCCTATTCC TGATTTTTAT 
ACACCCGGCG AGTCTGAAGC TGCAGGCTCA GTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157043.1
CDS	402..2540
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEMSLPSYGSQTLATVAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAITDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156986.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156986.3

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATAA CTGATGGAGG GATAAGTAAA 
GGAGCTACGG TGGGTGTTTT GCTGGATTTC ACCCGTGGGA TTCTCTTGTT TTTGATAAAT 
GATGAGCAAC AGGGACCCGT TGCCTTCAAT ACACTGGAGG GCATGTACTA CCCAGCCATC 
AGCCTCAACC GCAATGTTCA GGTGACTCTT CATTCTGGGC TGCCTATTCC TGATTTTTAT 
ACACCCGGCG AGTCTGAAGC TGCAGGCTCA GTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_005156986.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157043.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa18977
Clone ID Related Accession (Same CDS sequence)	XM_005156986.3 , XM_005156986.4
Accession Version	XM_005156986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156986.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATAA CTGATGGAGG GATAAGTAAA 
GGAGCTACGG TGGGTGTTTT GCTGGATTTC ACCCGTGGGA TTCTCTTGTT TTTGATAAAT 
GATGAGCAAC AGGGACCCGT TGCCTTCAAT ACACTGGAGG GCATGTACTA CCCAGCCATC 
AGCCTCAACC GCAATGTTCA GGTGACTCTT CATTCTGGGC TGCCTATTCC TGATTTTTAT 
ACACCCGGCG AGTCTGAAGC TGCAGGCTCA GTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157043.1
CDS	683..2821
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSEMSLPSYGSQTLATVAWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAITDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_005156986.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156986.4

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAT GAGTCTTCCT 
TCATATGGTA GTCAAACTCT GGCAACAGTA GCCTGGTTCA CCTTTGACTC CGCATCGGCT 
CATCCTGACA TCATCTTGTC CAATGACAAC CTCACGGTGA CGTGTAACAG CTACGACGAC 
CGCGTGGTCA TGGGAAACAC TGGCTTTTCT CGTGGCGTGC ATTACTGGGA GATGACCATC 
GATCGCTATG ATAACCACCC TGACCCAGCG TTTGGCGTCG CCCGGGCCGA TGTGATGAAG 
GATGTGATGC TGGGCAAGGA TGATAAAGCA TGGGCCATAA CTGATGGAGG GATAAGTAAA 
GGAGCTACGG TGGGTGTTTT GCTGGATTTC ACCCGTGGGA TTCTCTTGTT TTTGATAAAT 
GATGAGCAAC AGGGACCCGT TGCCTTCAAT ACACTGGAGG GCATGTACTA CCCAGCCATC 
AGCCTCAACC GCAATGTTCA GGTGACTCTT CATTCTGGGC TGCCTATTCC TGATTTTTAT 
ACACCCGGCG AGTCTGAAGC TGCAGGCTCA GTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim9 ( XM_009307466.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305741.1 Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.

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CloneID	ODa40061
Clone ID Related Accession (Same CDS sequence)	XM_009307466.2
Accession Version	XM_009307466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TRIM9 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAC CTGGTTCACC 
TTTGACTCCG CATCGGCTCA TCCTGACATC ATCTTGTCCA ATGACAACCT CACGGTGACG 
TGTAACAGCT ACGACGACCG CGTGGTCATG GGAAACACTG GCTTTTCTCG TGGCGTGCAT 
TACTGGGAGA TGACCATCGA TCGCTATGAT AACCACCCTG ACCCAGCGTT TGGCGTCGCC 
CGGGCCGATG TGATGAAGGA TGTGATGCTG GGCAAGGATG ATAAAGCATG GGCCATGTAT 
GTGGACAACA ACCGCTCCTG GTTCATGCAT AACAACTCAC ACACCAACCG AACTGATGGA 
GGGATAAGTA AAGGAGCTAC GGTGGGTGTT TTGCTGGATT TCACCCGTGG GATTCTCTTG 
TTTTTGATAA ATGATGAGCA ACAGGGACCC GTTGCCTTCA ATACACTGGA GGGCATGTAC 
TACCCAGCCA TCAGCCTCAA CCGCAATGTT CAGGTGACTC TTCATTCTGG GCTGCCTATT 
CCTGATTTTT ATACACCCGG CGAGTCTGAA GCTGCAGGCT CAGTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305741.1
CDS	398..2548
Translation	MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSSRASGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFVSAPTTPCQKSPNGVRVFPPSMPPPQQAQHHLLPHTGVLPPVPRNSCLTCPQCHRSLTLDDRGLRGFPKNRVLEGVVDRYQQSKAAALKCQLCEKSPREATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLVPPAQGRISRRASPRKISTCTEHELENLSMYCVQCKTPVCYQCLEDGKHSTHEVKALGAMWKLHKGQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLSRVTKEHEHKLSVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTENQWGKGSLTPRMSSDFDLTLDSGPLLQTIHQLDFVQMKVPAAPILQLEECCTQTSSATLSWKQPPLSTIAVDGYILELDDGTGGQFREVYVGTEMICTVDGLHFNSTYKSRVKAFNSSGVGQYSKTLVMQTSETWFTFDSASAHPDIILSNDNLTVTCNSYDDRVVMGNTGFSRGVHYWEMTIDRYDNHPDPAFGVARADVMKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGISKGATVGVLLDFTRGILLFLINDEQQGPVAFNTLEGMYYPAISLNRNVQVTLHSGLPIPDFYTPGESEAAGSVC

Target ORF information:

RefSeq Version	XM_009307466.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tripartite motif containing 9 (trim9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307466.2

ORF Insert Sequence:

ATGGATGAAA TGGAAGAGGA GTTAAAATGC CCGGTGTGCG GCTCATTTTT CCGCGAGCCG 
ATCATCCTGC CCTGCTCGCA TAATATTTGC CTGGCCTGCG CGAGAAACAT CCTGGTACAG 
ACGCCCGACG CCGAATCCCC GCAAAGCAGC CGCGCGTCCG GCTCCGGGGT CTCCGATTAT 
GATTACTTAG ACCTGGATAA GATGAGTCTG TACAGCGAGG CGGACAGCGG ATACGGCTCT 
TACGGCGGGT TCGTGAGCGC ACCGACCACC CCTTGCCAGA AGTCCCCCAA CGGCGTGCGT 
GTTTTCCCTC CTTCAATGCC TCCTCCACAG CAGGCGCAAC ACCACCTGCT GCCTCACACC 
GGCGTCCTGC CGCCAGTCCC ACGGAACTCC TGCCTCACCT GCCCGCAGTG CCACCGCAGC 
CTGACGCTGG ATGACAGGGG GCTCCGGGGG TTCCCCAAGA ACCGCGTTTT GGAGGGGGTG 
GTGGACCGGT ATCAGCAGAG CAAAGCCGCC GCGCTCAAGT GTCAACTGTG CGAAAAGAGC 
CCCCGCGAGG CGACTGTCAT GTGCGAGCAG TGCGACGTGT TTTATTGCGA CCCCTGCCGC 
CTCCGGTGCC ACCCTCCCCG AGGCCCGTTG GCCAAGCACC GGCTCGTGCC GCCGGCGCAA 
GGCAGAATCA GCCGCCGCGC GAGCCCGCGG AAAATCTCCA CCTGCACCGA GCACGAGCTG 
GAAAACCTGA GTATGTACTG TGTCCAATGT AAGACCCCCG TGTGCTACCA GTGTCTGGAG 
GACGGCAAAC ACTCCACCCA TGAGGTCAAA GCGCTGGGAG CCATGTGGAA GCTCCACAAG 
GGACAACTCT CTCAAGCACT GAATGTTCTG TCTGATCGGG CCACAGAGGC TAAAGAGTTC 
CTGGTGCAGC TCAGAAATAT GGTCCAACAC ATACAGGAGA ACGGAGTTGA ATTTGAGGCG 
TGTTTGGTGG CCCAGTGTGA TGCTCTGATT GAAGCTCTGA ACCGCAGGAA AGCACAGCTT 
CTCAGCAGAG TAACCAAAGA ACATGAACAC AAACTCTCGG TGGTTCGTGA TCAGATCTCG 
CACTGCACGG TGAAGTTGAG GCAGACCACA GGACTAATGG AGTACTGTCT GGAGGTTATC 
AAAGAGAACG ATCCCAGCGG CTTCCTGCAG ATCTCGGATG CTCTGATCAG ACGGGTCCAT 
ATGACGGAGA ATCAGTGGGG GAAAGGCTCT CTGACCCCCC GCATGAGCAG TGACTTTGAC 
CTGACGCTGG ACAGCGGGCC TCTGCTGCAG ACCATACACC AGCTAGACTT TGTGCAGATG 
AAGGTTCCAG CTGCTCCTAT TCTTCAGTTA GAGGAGTGCT GCACTCAAAC CAGCAGCGCC 
ACTCTGTCCT GGAAACAGCC GCCCCTCTCC ACCATCGCTG TGGACGGGTA TATTCTAGAG 
CTGGATGATG GCACTGGAGG CCAGTTCAGA GAAGTGTACG TTGGGACTGA GATGATCTGC 
ACTGTGGACG GTCTGCACTT CAACAGTACT TACAAATCTC GTGTTAAAGC ATTTAACTCC 
AGCGGAGTCG GCCAGTACAG CAAAACGCTT GTCATGCAGA CGTCAGAGAC CTGGTTCACC 
TTTGACTCCG CATCGGCTCA TCCTGACATC ATCTTGTCCA ATGACAACCT CACGGTGACG 
TGTAACAGCT ACGACGACCG CGTGGTCATG GGAAACACTG GCTTTTCTCG TGGCGTGCAT 
TACTGGGAGA TGACCATCGA TCGCTATGAT AACCACCCTG ACCCAGCGTT TGGCGTCGCC 
CGGGCCGATG TGATGAAGGA TGTGATGCTG GGCAAGGATG ATAAAGCATG GGCCATGTAT 
GTGGACAACA ACCGCTCCTG GTTCATGCAT AACAACTCAC ACACCAACCG AACTGATGGA 
GGGATAAGTA AAGGAGCTAC GGTGGGTGTT TTGCTGGATT TCACCCGTGG GATTCTCTTG 
TTTTTGATAA ATGATGAGCA ACAGGGACCC GTTGCCTTCA ATACACTGGA GGGCATGTAC 
TACCCAGCCA TCAGCCTCAA CCGCAATGTT CAGGTGACTC TTCATTCTGG GCTGCCTATT 
CCTGATTTTT ATACACCCGG CGAGTCTGAA GCTGCAGGCT CAGTCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of Trim9 function abrogates macrophage motility in vivo.
Journal of leukocyte biology102(6)1371-1380(2017 Dec)
Tokarz DA,Heffelfinger AK,Jima DD,Gerlach J,Shah RN,Rodriguez-Nunez I,Kortum AN,Fletcher AA,Nordone SK,Law JM,Heber S,Yoder JA

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Scientific reports630213(2016 Jul)
Loponte S,Segr? CV,Senese S,Miccolo C,Santaguida S,Deflorian G,Citro S,Mattoscio D,Pisati F,Moser MA,Visintin R,Seiser C,Chiocca S

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Novel cardiovascular gene functions revealed via systematic phenotype prediction in zebrafish.
Development (Cambridge, England)141(1)224-35(2014 Jan)
Musso G,Tasan M,Mosimann C,Beaver JE,Plovie E,Carr LA,Chua HN,Dunham J,Zuberi K,Rodriguez H,Morris Q,Zon L,Roth FP,MacRae CA

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trim9 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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