slc1a2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc1a2b
Entrez Gene ID	335836
Full Name	solute carrier family 1 (glial high affinity glutamate transporter), member 2b
Synonyms	eaat2,fj34b12,slc1a2,wu:fj34b12,zgc:65897
General protein information	Preferred Names solute carrier family 1 (glial high affinity glutamate transporter), member 2b Names excitatory amino acid transporter 2 EAAT2a SLC1A2a solute carrier family 1 (glial high affinity glutamate transporter), member 2 tnt
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298221.3	XP_009296496.1	excitatory amino acid transporter 2 isoform X1
XM_009298222.2	XP_009296497.1	excitatory amino acid transporter 2 isoform X1
XM_009298220.2	XP_009296495.1	excitatory amino acid transporter 2 isoform X2
XM_009298222.3	XP_009296497.1	excitatory amino acid transporter 2 isoform X1
XM_009298220.3	XP_009296495.1	excitatory amino acid transporter 2 isoform X2
XM_009298221.2	XP_009296496.1	excitatory amino acid transporter 2 isoform X1
NM_199979.2	NP_956273.2	excitatory amino acid transporter 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC1A2	NP_001003138.1
Bos taurus (cattle)	SLC1A2	XP_002693569.2
Rattus norvegicus (Norway rat)	Slc1a2	NP_058911.2
	SLC1A2	XP_001115008.1
Mus musculus (house mouse)	Slc1a2	NP_001070982.1
Xenopus tropicalis (tropical clawed frog)	slc1a2	XP_002937340.1
Homo sapiens (human)	SLC1A2	NP_004162.2
Gallus gallus (chicken)	SLC1A2	NP_001012917.1
Pan troglodytes (chimpanzee)	SLC1A2	XP_003313030.1
Danio rerio (zebrafish)	slc1a2b	NP_956273.1
Caenorhabditis elegans (roundworm)	glt-1	NP_001024393.1

Gene Ontology
Bibliography

Related articles in PubMed

Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters.
Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF
eNeuro4(3)(2017 May-Jun)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish.
Oosterhof N, Holtman IR, Kuil LE, van der Linde HC, Boddeke EW, Eggen BJ, van Ham TJ
Glia65(1)138-149(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish.
Title: Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish.

The following slc1a2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc1a2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40054	NM_199979.2 Latest version!	Danio rerio solute carrier family 1 member 2b (slc1a2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40054	XM_009298220.3 Latest version!	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40055	XM_009298221.3 Latest version!	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40055	XM_009298222.3 Latest version!	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa40055	XM_009298222.2	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40054	XM_009298220.2	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40055	XM_009298221.2	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc1a2b ( NM_199979.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956273.2 Danio rerio solute carrier family 1 member 2b (slc1a2b), mRNA.

Price & Availability↑

CloneID	ODa40054
Clone ID Related Accession (Same CDS sequence)	XM_009298220.2 , XM_009298220.3 , NM_199979.2
Accession Version	NM_199979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Danio rerio(zebrafish)
Product	excitatory amino acid transporter 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU207301.28 and CU928084.13. On Jun 5, 2015 this sequence version replaced NM_199979.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056751.1, GFIL01038291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956273.2
CDS	265..1953
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQCVFAAQNTGLVDDCKVTLAATNGSTAEITVVEEEPWKND

Target ORF information:

RefSeq Version	NM_199979.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 1 member 2b (slc1a2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199979.2

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298220.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296495.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40054
Clone ID Related Accession (Same CDS sequence)	XM_009298220.2 , XM_009298220.3 , NM_199979.2
Accession Version	XM_009298220.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	excitatory amino acid transporter 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296495.1
CDS	45..1733
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQCVFAAQNTGLVDDCKVTLAATNGSTAEITVVEEEPWKND

Target ORF information:

RefSeq Version	XM_009298220.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298220.2

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298220.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296495.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40054
Clone ID Related Accession (Same CDS sequence)	XM_009298220.2 , XM_009298220.3 , NM_199979.2
Accession Version	XM_009298220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	excitatory amino acid transporter 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296495.1
CDS	50..1738
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQCVFAAQNTGLVDDCKVTLAATNGSTAEITVVEEEPWKND

Target ORF information:

RefSeq Version	XM_009298220.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298220.3

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT GCGTCTTTGC TGCTCAAAAT ACAGGCTTAG TAGATGATTG CAAGGTAACC 
TTGGCGGCCA CCAACGGCTC GACTGCGGAG ATCACAGTCG TTGAGGAAGA ACCCTGGAAA 
AACGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298221.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296496.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40055
Clone ID Related Accession (Same CDS sequence)	XM_009298221.3 , XM_009298222.2 , XM_009298222.3 , XM_009298221.2
Accession Version	XM_009298221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	excitatory amino acid transporter 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296496.1
CDS	29..1600
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQ

Target ORF information:

RefSeq Version	XM_009298221.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298221.3

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298222.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296497.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40055
Clone ID Related Accession (Same CDS sequence)	XM_009298221.3 , XM_009298222.2 , XM_009298222.3 , XM_009298221.2
Accession Version	XM_009298222.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	excitatory amino acid transporter 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296497.1
CDS	262..1833
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQ

Target ORF information:

RefSeq Version	XM_009298222.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298222.2

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298222.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296497.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40055
Clone ID Related Accession (Same CDS sequence)	XM_009298221.3 , XM_009298222.2 , XM_009298222.3 , XM_009298221.2
Accession Version	XM_009298222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	excitatory amino acid transporter 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296497.1
CDS	216..1787
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQ

Target ORF information:

RefSeq Version	XM_009298222.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298222.3

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc1a2b ( XM_009298221.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296496.1 Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.

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CloneID	ODa40055
Clone ID Related Accession (Same CDS sequence)	XM_009298221.3 , XM_009298222.2 , XM_009298222.3 , XM_009298221.2
Accession Version	XM_009298221.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	excitatory amino acid transporter 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296496.1
CDS	27..1598
Translation	MMPKHVEVRMHESHLEPIEARPLSKWGTICQKMRKNLLLTLTVMGVIMGAVSGMLLRVASPIDDNIVMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKSSGRLGSRAMIYYMTTTVIAAVLGVILVLLIHPGNPKLKENLGEGLENDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVVKKVEVQPDLDDTNSTMSEYLSNATKPPPIFKDKKSLQFKSGMNVLGLIGFFIAFGICMGKMGEKARLMIDFFNILNEIVMKLVIMIMWYSPFGIACLICGKIISIKDLEVVARQLGMYMITVIVGLIIHGAIFLPCIYFAIVRKNPFSFFMGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGIDLDPGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSYGAGIVYHLSKAELDALDAQHGKTDDIEMMTKTQSYYDDLKNHHENNSNQ

Target ORF information:

RefSeq Version	XM_009298221.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 1 (glial high affinity glutamate transporter), member 2b (slc1a2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298221.2

ORF Insert Sequence:

ATGATGCCGA AGCACGTGGA GGTACGGATG CACGAAAGCC ACCTGGAGCC CATCGAGGCC 
CGGCCGCTGT CCAAATGGGG CACGATCTGC CAGAAGATGC GGAAGAACCT GCTGCTCACG 
CTGACCGTGA TGGGTGTGAT TATGGGCGCC GTGTCGGGAA TGCTGCTGCG TGTGGCGTCT 
CCCATCGATG ACAACATTGT GATGGTGATC GCCTTCCCTG GAGATATCCT GATGAGGATG 
CTGAAGATGC TGATCCTGCC CTTGATCATC TCCAGTCTCA TCACAGGTCT GGCTGGTCTG 
GATGCCAAGT CGAGCGGCCG TCTGGGCTCC AGAGCCATGA TCTACTACAT GACCACCACA 
GTGATCGCAG CGGTGCTGGG GGTCATCCTG GTGCTGCTCA TCCACCCCGG GAACCCCAAA 
CTCAAGGAGA ATCTGGGAGA AGGACTCGAG AACGACGAGG TGTCCAGTCT GGACGCTTTC 
TTTGACCTGA TCAGAAACCT GTTCCCCGAG AACCTGGTCC AGGCCTGTTT CCAGCAGATC 
CAGACGGTGG TGAAGAAGGT GGAGGTTCAG CCGGATCTGG ACGACACCAA CAGCACCATG 
TCGGAGTATC TGTCCAACGC CACCAAACCT CCACCCATCT TCAAAGACAA GAAATCCCTG 
CAGTTCAAGA GCGGCATGAA TGTGCTGGGT CTGATCGGAT TCTTCATCGC CTTTGGTATT 
TGTATGGGAA AGATGGGGGA GAAGGCGAGA CTGATGATCG ACTTCTTCAA CATCCTCAAT 
GAGATCGTCA TGAAGCTGGT CATCATGATC ATGTGGTATT CTCCATTCGG CATCGCCTGC 
CTGATCTGTG GGAAGATCAT CTCCATCAAG GATCTGGAGG TGGTGGCGCG GCAGCTGGGC 
ATGTACATGA TCACGGTCAT CGTGGGTCTG ATCATCCACG GAGCCATATT CCTGCCCTGC 
ATCTACTTCG CCATCGTCAG GAAAAACCCC TTCTCCTTCT TCATGGGCAT CTTCCAGGCC 
TGGATCACCG CTCTGGGAAC CGCTTCTAGT GCTGGAACTC TGCCCGTGAC GTTCCGCTGT 
CTGGAGGAGA ACCTGGGCAT TGATAAGAGA GTCACGCGCT TCGTTCTTCC AGTCGGAGCC 
ACCATCAACA TGGACGGAAC GGCGCTCTAT GAGGCGGTCG CCGCCATTTT CATCGCACAG 
ATGAACGGGA TCGATCTAGA CCCTGGACAG ATCGTCACCG TCAGTCTTAC AGCAACACTC 
GCCAGTGTGG GAGCCGCTAG TATTCCCAGT GCTGGACTGG TCACCATGCT GCTCATCCTG 
ACGGCGGTGG GTCTGCCCAC TCAGGACATC AGTCTGCTGG TGGCGGTGGA CTGGCTGCTG 
GACCGCTTCC GTACCTCAGT GAATGTGGTG GGAGACTCGT ACGGCGCAGG TATCGTCTAT 
CACCTGTCCA AGGCAGAGCT GGACGCTCTG GACGCCCAGC ACGGGAAGAC TGACGACATC 
GAGATGATGA CCAAGACCCA GTCCTACTAT GATGACCTCA AAAACCATCA TGAAAACAAC 
TCCAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters.
eNeuro4(3)(2017 May-Jun)
Niklaus S,Cadetti L,Vom Berg-Maurer CM,Lehnherr A,Hotz AL,Forster IC,Gesemann M,Neuhauss SCF

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish.
Glia65(1)138-149(2017 Jan)
Oosterhof N,Holtman IR,Kuil LE,van der Linde HC,Boddeke EW,Eggen BJ,van Ham TJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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slc1a2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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