mdm4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mdm4
Entrez Gene ID	334932
Full Name	MDM4, p53 regulator
Synonyms	wu:fa09h09,wu:fi33d10
General protein information	Preferred Names MDM4, p53 regulator Names protein Mdm4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein double minute 4 protein mdm2-like p53-binding protein p53-binding protein Mdm4 protein Mdmx transformed 3T3 cell double minute 4 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172869.3	XP_005172926.1	protein Mdm4 isoform X1
XM_005172871.3	XP_005172928.1	protein Mdm4 isoform X2
NM_212732.2	NP_997897.2	protein Mdm4 isoform 2
NM_001328581.1	NP_001315510.1	protein Mdm4 isoform 1
XM_005172869.4	XP_005172926.1	protein Mdm4 isoform X1
XM_005172871.4	XP_005172928.1	protein Mdm4 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MDM4	XP_004934981.1
Xenopus tropicalis (tropical clawed frog)	mdm4	NP_001135717.1
Rattus norvegicus (Norway rat)	Mdm4	NP_001012026.1
Canis lupus familiaris (dog)	MDM4	XP_005640824.1
Bos taurus (cattle)	MDM4	NP_001039634.1
Mus musculus (house mouse)	Mdm4	NP_032601.2
Danio rerio (zebrafish)	mdm4	NP_997897.1
Pan troglodytes (chimpanzee)	MDM4	XP_003949728.1
	MDM4	XP_001097488.2
Homo sapiens (human)	MDM4	NP_002384.2

Gene Ontology
Bibliography

Related articles in PubMed

Tumor-specific signaling to p53 is mimicked by Mdm2 inactivation in zebrafish: insights from mdm2 and mdm4 mutant zebrafish.
Chua JS, Liew HP, Guo L, Lane DP
Oncogene34(48)5933-41(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Ionizing radiation induces a dramatic persistence of p53 protein accumulation and DNA damage signaling in mutant p53 zebrafish.
Guo L, Liew HP, Camus S, Goh AM, Chee LL, Lunny DP, Lane EB, Lane DP
Oncogene32(34)4009-16(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that knockdown of the Mdm2 and Mdm4 caused dramatic accumulation of mutant p53 protein.
Title: Ionizing radiation induces a dramatic persistence of p53 protein accumulation and DNA damage signaling in mutant p53 zebrafish.

crystal structure of N-terminal domain of Mdmx bound to 15-residue p53 peptide was determined; structure reveals that although principle features of Mdm2-p53 interaction are preserved, the Mdmx hydrophobic cleft on which the p53 peptide binds is altered
Title: Molecular basis for the inhibition of p53 by Mdmx.

The following mdm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mdm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17028	NM_001328581.1 Latest version!	Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa17029	NM_212732.2 Latest version!	Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa17028	XM_005172869.4 Latest version!	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa17029	XM_005172871.4 Latest version!	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa17028	XM_005172869.3	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa17029	XM_005172871.3	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mdm4 ( NM_001328581.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315510.1 Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa17028
Clone ID Related Accession (Same CDS sequence)	XM_005172869.3 , NM_001328581.1 , XM_005172869.4
Accession Version	NM_001328581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	protein Mdm4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX545854.10 and BX571949.21. On Jun 18, 2016 this sequence version replaced XM_005172870.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC097069.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315510.1
CDS	161..1651
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQLSPGQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	NM_001328581.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328581.1

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdm4 ( NM_212732.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997897.2 Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa17029
Clone ID Related Accession (Same CDS sequence)	XM_005172871.3 , NM_212732.2 , XM_005172871.4
Accession Version	NM_212732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	protein Mdm4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX545854.10 and BX571949.21. On Jun 18, 2016 this sequence version replaced NM_212732.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC047801.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997897.2
CDS	161..1636
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	NM_212732.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MDM4 regulator of p53 (mdm4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212732.2

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdm4 ( XM_005172869.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172926.1 Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17028
Clone ID Related Accession (Same CDS sequence)	XM_005172869.3 , NM_001328581.1 , XM_005172869.4
Accession Version	XM_005172869.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Mdm4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172926.1
CDS	350..1840
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQLSPGQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	XM_005172869.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172869.3

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdm4 ( XM_005172869.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172926.1 Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17028
Clone ID Related Accession (Same CDS sequence)	XM_005172869.3 , NM_001328581.1 , XM_005172869.4
Accession Version	XM_005172869.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Mdm4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172869.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172926.1
CDS	350..1840
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQLSPGQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	XM_005172869.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172869.4

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
CTCTCTCCCG GACAGGACGA GGACACGGCC ATCGTGTCTG ACACCACAGA TGATCTTTGG 
TTCCTGAATG AGGCAGAGAG CGAGCAGGTG AGCGTGGAGA TGAAGGAGGC TGTGCTAGAA 
CAGGGCAGTG ATGGAGAGTC TCCGCATGAA GATGAGGACA CTGGGAAAGA CAGTAAAGAT 
GATGGAAAGA TGCAAGAAGA ACAGGAGGAA GACTCTCAGT GTTTAAGTGA TGACACAGAT 
ACTGAGATCT CCACACAGGA TGCATGGCAG TGTTCAGAGT GCCGCAAGTT TAACACCCCT 
CTTCAGCGAT ACTGCATGCG CTGCTGGGCT CTGCGGAAAG ACTGGTACAA GGATTGTCCT 
CGTCTCGTTC ACTCCATCTC AGTGCCAGAC ATCCCTGCCT GCAGTTCACG TCCTGAGAGG 
GATGAAGACG AGGAGGAGGA GGATGATGAT GGAATCGACA TGCCTGACTG CCTCAGGACT 
GTGTCTGACC CAGTCGTCCT CCCATCGCAT CGCGTCTCTA GAAACATCCC CTCATCATCC 
GCTTCCTCAT CTAAAGGAAA GGGTCCTTCC CAGATTCACC ATCACTTACA AGAGACATCA 
GAGGGGGATA GTCAGGACAC TCTGGACATG GAGACCGAAT ATCAACCTGA GGCCTTGTTG 
GAACCCTGCA AGCTGTGTCG TGTGCGGCCG CGAAATGGGA ACATCATCCA TGGCAGAACG 
GCACACCTGA TCACATGTTT CCCCTGTGCT CGCAAGCTGC ACAAATTTCA CGCTCCTTGC 
CCAGGCTGTG GCCAGGTTAT ACAGAAGGTC ATCAAAACCT TCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdm4 ( XM_005172871.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172928.1 Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17029
Clone ID Related Accession (Same CDS sequence)	XM_005172871.3 , NM_212732.2 , XM_005172871.4
Accession Version	XM_005172871.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Mdm4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172928.1
CDS	351..1826
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	XM_005172871.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172871.3

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdm4 ( XM_005172871.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172928.1 Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17029
Clone ID Related Accession (Same CDS sequence)	XM_005172871.3 , NM_212732.2 , XM_005172871.4
Accession Version	XM_005172871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Mdm4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172928.1
CDS	350..1825
Translation	MTSLASSSQLPGSCRTLPGEGTQVHPRAPLLQILKVAGAQEEVFTLKEVMHYLGQYIMMKQLYDKQRQHIVHCHDDPLGELLEVGSFSVKNPSPVYEMLKRNLVILNNSDAAKNLSVGKDSNESPSEDPGQVSSESINSAQPLIAGSSSTGTTQSCSQRRPRDPDEDSSDGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAVLEQGSDGESPHEDEDTGKDSKDDGKMQEEQEEDSQCLSDDTDTEISTQDAWQCSECRKFNTPLQRYCMRCWALRKDWYKDCPRLVHSISVPDIPACSSRPERDEDEEEEDDDGIDMPDCLRTVSDPVVLPSHRVSRNIPSSSASSSKGKGPSQIHHHLQETSEGDSQDTLDMETEYQPEALLEPCKLCRVRPRNGNIIHGRTAHLITCFPCARKLHKFHAPCPGCGQVIQKVIKTFIA

Target ORF information:

RefSeq Version	XM_005172871.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MDM4, p53 regulator (mdm4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172871.4

ORF Insert Sequence:

ATGACCTCAT TGGCATCGTC ATCTCAGTTA CCGGGGTCAT GCAGGACTCT TCCAGGGGAA 
GGGACTCAGG TCCACCCCAG AGCGCCTCTG CTGCAGATCT TGAAAGTAGC AGGTGCTCAG 
GAGGAGGTGT TTACCCTGAA GGAGGTCATG CATTATCTGG GTCAGTACAT CATGATGAAG 
CAGCTGTATG ACAAACAGAG GCAGCACATC GTCCACTGTC ACGATGACCC ACTGGGAGAG 
CTGCTGGAAG TGGGGAGCTT TTCTGTTAAA AACCCCAGCC CTGTGTATGA GATGTTGAAG 
AGGAATTTGG TAATTTTAAA TAATTCGGAT GCGGCAAAGA ATCTTTCTGT CGGCAAGGAT 
TCTAATGAGT CTCCAAGTGA AGATCCTGGT CAGGTGTCCA GTGAGTCAAT AAACTCTGCT 
CAGCCTCTCA TCGCTGGTAG TTCCAGTACT GGCACAACGC AGAGCTGCTC ACAGCGTAGG 
CCACGTGATC CAGATGAAGA CTCCTCAGAT GGTTTGCCCA GGTCTGCCTG CAAACGACCA 
AAGCTGGACG TGACTCTGGA AGAGTGGGAC CTTTCAGGGT TGCCTTGGTG GTTTTTGGGT 
AACTTGCGAA GTAATTACAC TCGGCGGAGC AACGGTTCCA CTGATATTCA CACCAATCAA 
GACGAGGACA CGGCCATCGT GTCTGACACC ACAGATGATC TTTGGTTCCT GAATGAGGCA 
GAGAGCGAGC AGGTGAGCGT GGAGATGAAG GAGGCTGTGC TAGAACAGGG CAGTGATGGA 
GAGTCTCCGC ATGAAGATGA GGACACTGGG AAAGACAGTA AAGATGATGG AAAGATGCAA 
GAAGAACAGG AGGAAGACTC TCAGTGTTTA AGTGATGACA CAGATACTGA GATCTCCACA 
CAGGATGCAT GGCAGTGTTC AGAGTGCCGC AAGTTTAACA CCCCTCTTCA GCGATACTGC 
ATGCGCTGCT GGGCTCTGCG GAAAGACTGG TACAAGGATT GTCCTCGTCT CGTTCACTCC 
ATCTCAGTGC CAGACATCCC TGCCTGCAGT TCACGTCCTG AGAGGGATGA AGACGAGGAG 
GAGGAGGATG ATGATGGAAT CGACATGCCT GACTGCCTCA GGACTGTGTC TGACCCAGTC 
GTCCTCCCAT CGCATCGCGT CTCTAGAAAC ATCCCCTCAT CATCCGCTTC CTCATCTAAA 
GGAAAGGGTC CTTCCCAGAT TCACCATCAC TTACAAGAGA CATCAGAGGG GGATAGTCAG 
GACACTCTGG ACATGGAGAC CGAATATCAA CCTGAGGCCT TGTTGGAACC CTGCAAGCTG 
TGTCGTGTGC GGCCGCGAAA TGGGAACATC ATCCATGGCA GAACGGCACA CCTGATCACA 
TGTTTCCCCT GTGCTCGCAA GCTGCACAAA TTTCACGCTC CTTGCCCAGG CTGTGGCCAG 
GTTATACAGA AGGTCATCAA AACCTTCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor-specific signaling to p53 is mimicked by Mdm2 inactivation in zebrafish: insights from mdm2 and mdm4 mutant zebrafish.
Oncogene34(48)5933-41(2015 Nov)
Chua JS,Liew HP,Guo L,Lane DP

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one9(12)e114632(2014)
Thomas HR,Percival SM,Yoder BK,Parant JM

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Ionizing radiation induces a dramatic persistence of p53 protein accumulation and DNA damage signaling in mutant p53 zebrafish.
Oncogene32(34)4009-16(2013 Aug)
Guo L,Liew HP,Camus S,Goh AM,Chee LL,Lunny DP,Lane EB,Lane DP

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mdm4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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