ap2b1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap2b1
Entrez Gene ID	334632
Full Name	adaptor-related protein complex 2, beta 1 subunit
Synonyms	fa16e07,wu:fa16c06,wu:fa16e07,wu:fc18c08,zgc:55659
General protein information	Preferred Names adaptor-related protein complex 2, beta 1 subunit Names AP-2 complex subunit beta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199919.2	NP_956213.2	AP-2 complex subunit beta

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AP2B1	XP_415772.2
Danio rerio (zebrafish)	ap2b1	NP_956213.2
Bos taurus (cattle)	AP2B1	NP_001068593.1
Xenopus tropicalis (tropical clawed frog)	ap2b1	NP_989206.1
Homo sapiens (human)	AP2B1	NP_001025177.1
Canis lupus familiaris (dog)	AP2B1	XP_853723.1
Rattus norvegicus (Norway rat)	Ap2b1	NP_542150.1
Pan troglodytes (chimpanzee)	AP2B1	XP_003953112.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Ma Z, Zhu P, Pang M, Guo L, Chang N, Zheng J, Zhu X, Gao C, Huang H, Cui Z, Xiong JW, Peng J, Chen J
Scientific reports7(1)10381(2017 Sep)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning.
Umasankar PK, Sanker S, Thieman JR, Chakraborty S, Wendland B, Tsang M, Traub LM
Nature cell biology14(5)488-501(2012 Apr)

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ, Giraldez AJ
Genes & development23(5)619-32(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ap2b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap2b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34375	NM_199919.2 Latest version!	Danio rerio adaptor related protein complex 2 subunit beta 1 (ap2b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap2b1 ( NM_199919.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956213.2 Danio rerio adaptor related protein complex 2 subunit beta 1 (ap2b1), mRNA.

Price & Availability↑

CloneID	ODa34375
Clone ID Related Accession (Same CDS sequence)	NM_199919.2
Accession Version	NM_199919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	AP-2 complex subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066566.1. On Apr 25, 2004 this sequence version replaced NM_199919.1. ##Evidence-Data-START## Transcript exon combination :: BC066566.1, BC049138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACTGACT CAAAGTATTT CACCACCAAC AAAAAAGGAG AAATCTTTGA GCTGAAGGCC 
GAGCTGAATA ATGAGAAGAA GGAGAAGAGG AAAGAGGCAG TGAAGAAAGT CATCGCCGCC 
ATGACGGTGG GCAAGGATGT CAGTTCGTTG TTTCCAGATG TGGTGAACTG CATGCAGACG 
GACAACCTGG AGCTGAAGAA GCTGGTTTAC CTGTACCTTA TGAATTATGC CAAGAGTCAG 
CCGGACATGG CCATCATGGC AGTTAACAGC TTTGTCAAGG ACTGTGAGGA TCCAAACCCT 
CTGATCCGTG CTCTGGCTGT GCGCACGATG GGTTGTATCC GCGTGGACAA GATCACTGAG 
TACCTCTGTG AACCTCTGCG AAAGTGTCTG AAGGATGAAG ACCCGTACGT GAGGAAGACT 
GCAGCCGTGT GTGTGGCCAA ACTGCATGAC ATCAATGCTC AGATGGTGGA GGACCAGGGC 
TTCCTGGACT CTCTCCGGGA CCTGATCGCT GACTCCAATC CCATGGTTGT GGCCAATGCA 
GTTGCTGCTC TGTCTGAGAT CAGCGAGTCT CACCCCAACA GCAACCTTCT GGACCTGAAC 
CCTCAGAACA TCAACAAGCT TCTGACGGCT CTTAATGAGT GCACTGAATG GGGCCAGATC 
TTCATCCTGG ACTGCCTCTC CAACTACAAC CCCAAAGACG AGCGGGAGGC CCAGAGTATC 
TGTGAGCGGG TGACGCCACG TCTCTCGCAT GCTAATTCAG CTGTGGTGCT TTCGGCGGTA 
AAGGTACTGA TGAAGTTTTT GGAGCTCCTG CCCAAAGACT CTGATTACTA CAACACTCTT 
CTGAAGAAGC TCTCTCCTCC CCTCGTCACA CTGTTATCTG GCGAACCAGA GGTCCAATAT 
GTGGCGCTCA GGAATATCAA CCTTATTGTG CAGAAACGGC CTGAGATTTT GAAGCAAGAG 
ATTAAAGTGT TCTTTGTCAA GTACAATGAC CCTATATATG TCAAGTTGGA GAAACTGGAC 
ATCATGATCC GACTAGCCTC TCATGCCAAC ATCGCCCAGG TCCTGGCTGA GCTGAAGGAA 
TATGCCACTG AGGTGGATGT AGACTTTGTT CGGAAAGCCG TGCGAGCCAT TGGTCGCTGT 
GCCATCAAAG TGGAGCAATC CGCTGAGCGC TGTGTTAGCA CCCTGCTGGA CCTTATCCAG 
ACCAAAGTCA ACTACGTCGT GCAGGAAGCC ATTGTGGTCA TCAGGGACAT CTTCCGCAAA 
TATCCCAACA AGTATGAGAG CATAATCGCC ACTCTGTGTG AAAACCTGGA CTCTCTGGAT 
GAACCTGACG CTCGAGGAGC CATGATCTGG ATTGTTGGCG AATATGCAGA GCGTATCGAC 
AACGCTGATG AGCTACTGGA GAGTTTCCTC GAGGGCTTCC ATGATGAAAG CACTCAGGTC 
CAGTTGACTC TGCTGACAGC CATAGTGAAG CTGTTCCTGA AGAAGCCCTC TGAGACTCAG 
GAGCTGGTTC AGCAGGTGCT CAGTCTGGCC ACTCAGGACT CTGATAACCC TGACCTGCGT 
GACCGCGGCT ACATCTACTG GCGTCTGCTG TCCACTGATC CTGTCACCGC TAAAGAGGTG 
GTGCTGTCTG AGAAACCTCT GATCTCAGAG GAGACGGACC TGATTGAGCC CACCCTGCTG 
GACGAGCTCA TCTGCCACAT TGGCTCGCTG GCCTCAGTCT ACCACAAGCC CCCAAATGCC 
TTTGTGGAGG GAAGCCATGG CATTCATCGC AAACATCTGC CCATCCAGCA CGGCAGTATC 
GACACCGGGG AGAGTCCCGT CAGTACCGGC CCGGCTGCTT CCATTGATCA ACCCCAGGTC 
ATCCCATCTC AGGGTGACCT TCTGGGAGAC CTGCTTAATC TGGACCTCGG GCCACCGGTC 
AATGTGCCCC AGGTGTCCTC CATGCAGATA GCGGCTGTGG ATCTCCTGGG AGGTGGTCTT 
GACAGTTTGC TCGGGGGAGA TCTGGGAGGA GGTGTTGGGG GCAGTCCTGC AGTGGGCCAG 
AACTTCATCC CATCATCGGT GCCCAACACC TTTGCACCAT CTCCGACACC TGCCGCTCTT 
AGCAGCGGAC TCAATGACCT GTTTGAGTTG TCCACAGGAA TGGCCACTGC TACAGGAGGA 
TATGTGGCTG CTAAAACTGT GTGGCTGCCA GCAGTCAAGG CCAAAGGACT GGAGATTTCT 
GGAACCTTCT CTCGGCGTCA AGGGCACATG TATATGGACA TGACCTTCAC CAATAAAGCA 
TTGCAGCATA TGACTGACTT TGCCATCCAG TTCAACAAGA ACAGTTTTGG TGTAATCCCC 
ACCACACCTC TCCCCGTCCA CACTCCACTG ATGCCCAGTC AGAGCATCGA CATCTCCCTC 
CCGCTCAACA CCATCGGCCC TGTGATGAAA ATGGATCCAC TCAACAACCT ACAGGTGGCC 
GTGAAAAACA ATATTGATGT CTTCTACTTT AGCACGCTGA TCCCTCTCAA TGTCTTCTTC 
GTGGAGGATG GCAAAATGGA GCGTCAGGTG TTCTTGGCCA CATGGAAGGA CATTCCTAAT 
GAGAATGAGC TGCAATACCA GATCAAAGAC TGCCACTTAA ACGCAGATAC GGTGTCGGGC 
AAACTGCAGA GCAACAATGT CTACACCATC GCAAAGAGGA ACGTGGAAGG CCAGGACATG 
TTGTACCAGT CGCTCAAACT CACCAACGGG ATCTGGATTC TCGCCGAGCT CCGCATACAA 
CCCGGCAATC CCAATTACAC GCTGTCTCTG AAGTGCAGGG CTCCAGAGGT TTCTCAGTAC 
GTGTACCAGA TGTACGATGC CACTCTGAAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956213.2
CDS	119..2974
Translation	MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTALNECTEWGQIFILDCLSNYNPKDEREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNTLLKKLSPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASHANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRKYPNKYESIIATLCENLDSLDEPDARGAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVKLFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLLDELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIQHGSIDTGESPVSTGPAASIDQPQVIPSQGDLLGDLLNLDLGPPVNVPQVSSMQIAAVDLLGGGLDSLLGGDLGGGVGGSPAVGQNFIPSSVPNTFAPSPTPAALSSGLNDLFELSTGMATATGGYVAAKTVWLPAVKAKGLEISGTFSRRQGHMYMDMTFTNKALQHMTDFAIQFNKNSFGVIPTTPLPVHTPLMPSQSIDISLPLNTIGPVMKMDPLNNLQVAVKNNIDVFYFSTLIPLNVFFVEDGKMERQVFLATWKDIPNENELQYQIKDCHLNADTVSGKLQSNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTLSLKCRAPEVSQYVYQMYDATLKN

Target ORF information:

RefSeq Version	NM_199919.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adaptor related protein complex 2 subunit beta 1 (ap2b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199919.2

ORF Insert Sequence:

ATGACTGACT CAAAGTATTT CACCACCAAC AAAAAAGGAG AAATCTTTGA GCTGAAGGCC 
GAGCTGAATA ATGAGAAGAA GGAGAAGAGG AAAGAGGCAG TGAAGAAAGT CATCGCCGCC 
ATGACGGTGG GCAAGGATGT CAGTTCGTTG TTTCCAGATG TGGTGAACTG CATGCAGACG 
GACAACCTGG AGCTGAAGAA GCTGGTTTAC CTGTACCTTA TGAATTATGC CAAGAGTCAG 
CCGGACATGG CCATCATGGC AGTTAACAGC TTTGTCAAGG ACTGTGAGGA TCCAAACCCT 
CTGATCCGTG CTCTGGCTGT GCGCACGATG GGTTGTATCC GCGTGGACAA GATCACTGAG 
TACCTCTGTG AACCTCTGCG AAAGTGTCTG AAGGATGAAG ACCCGTACGT GAGGAAGACT 
GCAGCCGTGT GTGTGGCCAA ACTGCATGAC ATCAATGCTC AGATGGTGGA GGACCAGGGC 
TTCCTGGACT CTCTCCGGGA CCTGATCGCT GACTCCAATC CCATGGTTGT GGCCAATGCA 
GTTGCTGCTC TGTCTGAGAT CAGCGAGTCT CACCCCAACA GCAACCTTCT GGACCTGAAC 
CCTCAGAACA TCAACAAGCT TCTGACGGCT CTTAATGAGT GCACTGAATG GGGCCAGATC 
TTCATCCTGG ACTGCCTCTC CAACTACAAC CCCAAAGACG AGCGGGAGGC CCAGAGTATC 
TGTGAGCGGG TGACGCCACG TCTCTCGCAT GCTAATTCAG CTGTGGTGCT TTCGGCGGTA 
AAGGTACTGA TGAAGTTTTT GGAGCTCCTG CCCAAAGACT CTGATTACTA CAACACTCTT 
CTGAAGAAGC TCTCTCCTCC CCTCGTCACA CTGTTATCTG GCGAACCAGA GGTCCAATAT 
GTGGCGCTCA GGAATATCAA CCTTATTGTG CAGAAACGGC CTGAGATTTT GAAGCAAGAG 
ATTAAAGTGT TCTTTGTCAA GTACAATGAC CCTATATATG TCAAGTTGGA GAAACTGGAC 
ATCATGATCC GACTAGCCTC TCATGCCAAC ATCGCCCAGG TCCTGGCTGA GCTGAAGGAA 
TATGCCACTG AGGTGGATGT AGACTTTGTT CGGAAAGCCG TGCGAGCCAT TGGTCGCTGT 
GCCATCAAAG TGGAGCAATC CGCTGAGCGC TGTGTTAGCA CCCTGCTGGA CCTTATCCAG 
ACCAAAGTCA ACTACGTCGT GCAGGAAGCC ATTGTGGTCA TCAGGGACAT CTTCCGCAAA 
TATCCCAACA AGTATGAGAG CATAATCGCC ACTCTGTGTG AAAACCTGGA CTCTCTGGAT 
GAACCTGACG CTCGAGGAGC CATGATCTGG ATTGTTGGCG AATATGCAGA GCGTATCGAC 
AACGCTGATG AGCTACTGGA GAGTTTCCTC GAGGGCTTCC ATGATGAAAG CACTCAGGTC 
CAGTTGACTC TGCTGACAGC CATAGTGAAG CTGTTCCTGA AGAAGCCCTC TGAGACTCAG 
GAGCTGGTTC AGCAGGTGCT CAGTCTGGCC ACTCAGGACT CTGATAACCC TGACCTGCGT 
GACCGCGGCT ACATCTACTG GCGTCTGCTG TCCACTGATC CTGTCACCGC TAAAGAGGTG 
GTGCTGTCTG AGAAACCTCT GATCTCAGAG GAGACGGACC TGATTGAGCC CACCCTGCTG 
GACGAGCTCA TCTGCCACAT TGGCTCGCTG GCCTCAGTCT ACCACAAGCC CCCAAATGCC 
TTTGTGGAGG GAAGCCATGG CATTCATCGC AAACATCTGC CCATCCAGCA CGGCAGTATC 
GACACCGGGG AGAGTCCCGT CAGTACCGGC CCGGCTGCTT CCATTGATCA ACCCCAGGTC 
ATCCCATCTC AGGGTGACCT TCTGGGAGAC CTGCTTAATC TGGACCTCGG GCCACCGGTC 
AATGTGCCCC AGGTGTCCTC CATGCAGATA GCGGCTGTGG ATCTCCTGGG AGGTGGTCTT 
GACAGTTTGC TCGGGGGAGA TCTGGGAGGA GGTGTTGGGG GCAGTCCTGC AGTGGGCCAG 
AACTTCATCC CATCATCGGT GCCCAACACC TTTGCACCAT CTCCGACACC TGCCGCTCTT 
AGCAGCGGAC TCAATGACCT GTTTGAGTTG TCCACAGGAA TGGCCACTGC TACAGGAGGA 
TATGTGGCTG CTAAAACTGT GTGGCTGCCA GCAGTCAAGG CCAAAGGACT GGAGATTTCT 
GGAACCTTCT CTCGGCGTCA AGGGCACATG TATATGGACA TGACCTTCAC CAATAAAGCA 
TTGCAGCATA TGACTGACTT TGCCATCCAG TTCAACAAGA ACAGTTTTGG TGTAATCCCC 
ACCACACCTC TCCCCGTCCA CACTCCACTG ATGCCCAGTC AGAGCATCGA CATCTCCCTC 
CCGCTCAACA CCATCGGCCC TGTGATGAAA ATGGATCCAC TCAACAACCT ACAGGTGGCC 
GTGAAAAACA ATATTGATGT CTTCTACTTT AGCACGCTGA TCCCTCTCAA TGTCTTCTTC 
GTGGAGGATG GCAAAATGGA GCGTCAGGTG TTCTTGGCCA CATGGAAGGA CATTCCTAAT 
GAGAATGAGC TGCAATACCA GATCAAAGAC TGCCACTTAA ACGCAGATAC GGTGTCGGGC 
AAACTGCAGA GCAACAATGT CTACACCATC GCAAAGAGGA ACGTGGAAGG CCAGGACATG 
TTGTACCAGT CGCTCAAACT CACCAACGGG ATCTGGATTC TCGCCGAGCT CCGCATACAA 
CCCGGCAATC CCAATTACAC GCTGTCTCTG AAGTGCAGGG CTCCAGAGGT TTCTCAGTAC 
GTGTACCAGA TGTACGATGC CACTCTGAAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Scientific reports7(1)10381(2017 Sep)
Ma Z,Zhu P,Pang M,Guo L,Chang N,Zheng J,Zhu X,Gao C,Huang H,Cui Z,Xiong JW,Peng J,Chen J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning.
Nature cell biology14(5)488-501(2012 Apr)
Umasankar PK,Sanker S,Thieman JR,Chakraborty S,Wendland B,Tsang M,Traub LM

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Genes & development23(5)619-32(2009 Mar)
Mishima Y,Abreu-Goodger C,Staton AA,Stahlhut C,Shou C,Cheng C,Gerstein M,Enright AJ,Giraldez AJ

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ap2b1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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