prkcda cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkcda
Entrez Gene ID	334571
Full Name	protein kinase C, delta a
Synonyms	fb09d10,fv43b11,prkcd,wu:fb09d10,wu:fv43b11,zgc:56558
General protein information	Preferred Names protein kinase C, delta a Names protein kinase C delta type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214708.1	NP_999873.1	protein kinase C delta type

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkcd	NP_035233.1
Drosophila melanogaster (fruit fly)	Pkcdelta	NP_511171.3
	LOC694656	XP_001083320.1
Canis lupus familiaris (dog)	PRKCD	NP_001008716.1
Danio rerio (zebrafish)	LOC100150844	XP_001919906.4
Xenopus tropicalis (tropical clawed frog)	prkcd	XP_002934267.1
Homo sapiens (human)	PRKCD	NP_006245.2
Pan troglodytes (chimpanzee)	PRKCD	XP_003309918.2
Rattus norvegicus (Norway rat)	Prkcd	NP_579841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000418	XP_001688308.2
Bos taurus (cattle)	PRKCD	NP_001071323.1
Danio rerio (zebrafish)	prkcda	NP_999873.1
Gallus gallus (chicken)	PRKCD	NP_001006133.1
Caenorhabditis elegans (roundworm)	tpa-1	NP_499860.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death.
Qvit N, Joshi AU, Cunningham AD, Ferreira JC, Mochly-Rosen D
The Journal of biological chemistry291(26)13608-21(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prkcda gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkcda cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32617	NM_214708.1 Latest version!	Danio rerio protein kinase C, delta a (prkcda), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkcda ( NM_214708.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999873.1 Danio rerio protein kinase C, delta a (prkcda), mRNA.

Price & Availability↑

CloneID	ODa32617
Clone ID Related Accession (Same CDS sequence)	NM_214708.1
Accession Version	NM_214708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	protein kinase C delta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049327.1. ##Evidence-Data-START## Transcript exon combination :: BC049327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCTT TCCTGAGAAT TGCATTTAAT GCCTATGATC TTGGCACATT CTCGCCAATG 
GCTGAGGCAC CATTCTGTGC AGTCAAAATG AAGGAAGCTC TCAGCACTGA GAGAGGAAAA 
ACCCTTGTCC AGAAGAAACC CACCATGTAT CCTGCTTGGC GATCCAGTTT TGATGCGCAT 
ATTTTTGAAG GACGAGTCAT ACAAATACTA CTCATGAGAA CTGCAGAGGA ACCTTTAGCA 
GACGTTACTG TTGGCGTATC TGTGTTGGCT GAAAGATGCA AGAAGGCAAA CGGGCATGCA 
GAGTTTTGGG TTGATTTGCA GCCTTCAGGG AAGGTTATGA TGTCTGTACA ATTCTTTTTG 
GAGGATTCAG ATACAGGCTC AGTGTGTCGT CATTCAGTAG TGGCCAACGA AGAGATACCA 
ACCCTCAACC GCCGTCGTGG AGCAATTAAA CAGGCCAAAA TTCACTTCAT CAAAAACCAT 
GAGTTCATCG CCACCTTCTT CAGACAGCCC ACCTTCTGCT CCGTCTGCAG AGATTTTGTT 
TGGGGTCTCA ACAAGCAAGG ATATAAATGT AGACAATGCA ATGCAGCCAT CCACAAGAAG 
TGTATTGATA AAATTATTGG AAGGTGCACT GGCACTGCAG CCAATAGTCG AGATACAATG 
TTTCAGAAGG AGCGTTTTAA GATTGATATG CCACATCGCT TTAAGACTAA CAACTACATG 
AGTCCGACTT TTTGTGACCA CTGCGGCAGT CTGCTGTGGG GTCTTGTCAA ACAAGGCCTC 
AAATGTGAGG ACTGTTCCAT GAATGTCCAT CATAAATGCC AGACTAAAGT GGCTAACCTG 
TGTGGCATCA ACCAGAAACT GCTGGCAGAA GCTCTTACTC AAGTCTCAAC TAAATCATCC 
ACCAAGCGAC CTGACAACAA CACACAGGAT GTTGGTGTTT ACCAGGATTT TAATAAAAGC 
CCAGGACCTG ATGTTACTGA TGGGACTCCT TATGGTCATA TATGGGAAGG TTCAAGTCCT 
CGTCCTCCAT CTCGAATCAC CCACCAAACA CGTATTACTG CTGAGCATTT CATTTTCCAT 
AAAGTCCTGG GGAAGGGAAG CTTTGGCAAG GTGCTGTTGG CTGAGCTGAA AGGCACTGGT 
GAATGGTTTG CAGTGAAGGC ACTGAAGAAA GACGTGGTGC TGATGGATGA TGATGTGGAG 
TGCACCATGG TGGAGAAACG TGTGCTTGCT CTTGCCTGGG ACAATCCCTT TCTGACACAC 
CTCTACTCTA CATTTCAGAC CAAGGAGCAC TTATTCTTTG TGATGGAGTA TCTGAATGGA 
GGTGATCTTA TGTTTCACAT ACAGGAAAAA GGGCGTTTTG ATCTGTACAG AGCCACGTTC 
TACGCCGCTG AAATAGTGTG TGGCTTGCAG TTTCTCCATT CCAAAGGCAT TATTTACAGG 
GATCTCAAGC TGGATAATGT GATGTTAGAT GGAGATGGTC ACATAAAAAT AGCTGATTTT 
GGCATGTGCA AGGAGAATGT GTTTGGAGAC AATCGGGCCA CAACCTTCTG CGGAACTCCT 
GATTATATTG CTCCTGAGAT TCTGCTCGGT CAGCAGTACT CGTTCTCAGT AGACTGGTGG 
TCTTTTGGTG TGCTCGTCTA TGAGATGCTC ATTGGTCAAT CCCCATTCCA CGGCGATGAC 
GAGGACGAGC TGTTTGAATC GATCCGAATG GACACACCTC ACTATCCTCG CTGGATCTTA 
CTGGACACCA GAGACATGCT AGAAAGGCTG TTTGAACGTG ACCCATCACG CCGTTTAGGG 
ATTGTGGGTA ATATACGAGG CCATTCATTC TTCAAAACTG TCAACTGGGC TGCTCTAGAA 
GGGCGAGAAG TTGATCCTCC TTTCAAACCA AAAGTGAAAG CTCCAAATGA CTGCAGTAAC 
TTTGACCGGG AGTTTTTGAG TGAGAAACCT CGTCTCTCAC ACTGTGAGAA AGGCCTGATC 
GATTCTATGG ACCAGTGTGC TTTCGCCGGC TTCTCATTCA TTAATCCCAA AATGGAGCAT 
CTTATTACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999873.1
CDS	94..2148
Translation	MAPFLRIAFNAYDLGTFSPMAEAPFCAVKMKEALSTERGKTLVQKKPTMYPAWRSSFDAHIFEGRVIQILLMRTAEEPLADVTVGVSVLAERCKKANGHAEFWVDLQPSGKVMMSVQFFLEDSDTGSVCRHSVVANEEIPTLNRRRGAIKQAKIHFIKNHEFIATFFRQPTFCSVCRDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTMFQKERFKIDMPHRFKTNNYMSPTFCDHCGSLLWGLVKQGLKCEDCSMNVHHKCQTKVANLCGINQKLLAEALTQVSTKSSTKRPDNNTQDVGVYQDFNKSPGPDVTDGTPYGHIWEGSSPRPPSRITHQTRITAEHFIFHKVLGKGSFGKVLLAELKGTGEWFAVKALKKDVVLMDDDVECTMVEKRVLALAWDNPFLTHLYSTFQTKEHLFFVMEYLNGGDLMFHIQEKGRFDLYRATFYAAEIVCGLQFLHSKGIIYRDLKLDNVMLDGDGHIKIADFGMCKENVFGDNRATTFCGTPDYIAPEILLGQQYSFSVDWWSFGVLVYEMLIGQSPFHGDDEDELFESIRMDTPHYPRWILLDTRDMLERLFERDPSRRLGIVGNIRGHSFFKTVNWAALEGREVDPPFKPKVKAPNDCSNFDREFLSEKPRLSHCEKGLIDSMDQCAFAGFSFINPKMEHLITK

Target ORF information:

RefSeq Version	NM_214708.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, delta a (prkcda), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214708.1

ORF Insert Sequence:

ATGGCCCCTT TCCTGAGAAT TGCATTTAAT GCCTATGATC TTGGCACATT CTCGCCAATG 
GCTGAGGCAC CATTCTGTGC AGTCAAAATG AAGGAAGCTC TCAGCACTGA GAGAGGAAAA 
ACCCTTGTCC AGAAGAAACC CACCATGTAT CCTGCTTGGC GATCCAGTTT TGATGCGCAT 
ATTTTTGAAG GACGAGTCAT ACAAATACTA CTCATGAGAA CTGCAGAGGA ACCTTTAGCA 
GACGTTACTG TTGGCGTATC TGTGTTGGCT GAAAGATGCA AGAAGGCAAA CGGGCATGCA 
GAGTTTTGGG TTGATTTGCA GCCTTCAGGG AAGGTTATGA TGTCTGTACA ATTCTTTTTG 
GAGGATTCAG ATACAGGCTC AGTGTGTCGT CATTCAGTAG TGGCCAACGA AGAGATACCA 
ACCCTCAACC GCCGTCGTGG AGCAATTAAA CAGGCCAAAA TTCACTTCAT CAAAAACCAT 
GAGTTCATCG CCACCTTCTT CAGACAGCCC ACCTTCTGCT CCGTCTGCAG AGATTTTGTT 
TGGGGTCTCA ACAAGCAAGG ATATAAATGT AGACAATGCA ATGCAGCCAT CCACAAGAAG 
TGTATTGATA AAATTATTGG AAGGTGCACT GGCACTGCAG CCAATAGTCG AGATACAATG 
TTTCAGAAGG AGCGTTTTAA GATTGATATG CCACATCGCT TTAAGACTAA CAACTACATG 
AGTCCGACTT TTTGTGACCA CTGCGGCAGT CTGCTGTGGG GTCTTGTCAA ACAAGGCCTC 
AAATGTGAGG ACTGTTCCAT GAATGTCCAT CATAAATGCC AGACTAAAGT GGCTAACCTG 
TGTGGCATCA ACCAGAAACT GCTGGCAGAA GCTCTTACTC AAGTCTCAAC TAAATCATCC 
ACCAAGCGAC CTGACAACAA CACACAGGAT GTTGGTGTTT ACCAGGATTT TAATAAAAGC 
CCAGGACCTG ATGTTACTGA TGGGACTCCT TATGGTCATA TATGGGAAGG TTCAAGTCCT 
CGTCCTCCAT CTCGAATCAC CCACCAAACA CGTATTACTG CTGAGCATTT CATTTTCCAT 
AAAGTCCTGG GGAAGGGAAG CTTTGGCAAG GTGCTGTTGG CTGAGCTGAA AGGCACTGGT 
GAATGGTTTG CAGTGAAGGC ACTGAAGAAA GACGTGGTGC TGATGGATGA TGATGTGGAG 
TGCACCATGG TGGAGAAACG TGTGCTTGCT CTTGCCTGGG ACAATCCCTT TCTGACACAC 
CTCTACTCTA CATTTCAGAC CAAGGAGCAC TTATTCTTTG TGATGGAGTA TCTGAATGGA 
GGTGATCTTA TGTTTCACAT ACAGGAAAAA GGGCGTTTTG ATCTGTACAG AGCCACGTTC 
TACGCCGCTG AAATAGTGTG TGGCTTGCAG TTTCTCCATT CCAAAGGCAT TATTTACAGG 
GATCTCAAGC TGGATAATGT GATGTTAGAT GGAGATGGTC ACATAAAAAT AGCTGATTTT 
GGCATGTGCA AGGAGAATGT GTTTGGAGAC AATCGGGCCA CAACCTTCTG CGGAACTCCT 
GATTATATTG CTCCTGAGAT TCTGCTCGGT CAGCAGTACT CGTTCTCAGT AGACTGGTGG 
TCTTTTGGTG TGCTCGTCTA TGAGATGCTC ATTGGTCAAT CCCCATTCCA CGGCGATGAC 
GAGGACGAGC TGTTTGAATC GATCCGAATG GACACACCTC ACTATCCTCG CTGGATCTTA 
CTGGACACCA GAGACATGCT AGAAAGGCTG TTTGAACGTG ACCCATCACG CCGTTTAGGG 
ATTGTGGGTA ATATACGAGG CCATTCATTC TTCAAAACTG TCAACTGGGC TGCTCTAGAA 
GGGCGAGAAG TTGATCCTCC TTTCAAACCA AAAGTGAAAG CTCCAAATGA CTGCAGTAAC 
TTTGACCGGG AGTTTTTGAG TGAGAAACCT CGTCTCTCAC ACTGTGAGAA AGGCCTGATC 
GATTCTATGG ACCAGTGTGC TTTCGCCGGC TTCTCATTCA TTAATCCCAA AATGGAGCAT 
CTTATTACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death.
The Journal of biological chemistry291(26)13608-21(2016 Jun)
Qvit N,Joshi AU,Cunningham AD,Ferreira JC,Mochly-Rosen D

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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prkcda cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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