clca1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clca1
Entrez Gene ID	334289
Full Name	chloride channel accessory 1
Synonyms	fi39d11,wu:fi39d11
General protein information	Preferred Names chloride channel accessory 1 Names calcium-activated chloride channel regulator 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305589.1	NP_001292518.1	calcium-activated chloride channel regulator 4 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC784768	NP_001229512.1
Danio rerio (zebrafish)	clca1	XP_002664610.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004059	XP_560062.4
Xenopus tropicalis (tropical clawed frog)	LOC100495107	XP_002931721.2
Canis lupus familiaris (dog)	LOC479964	XP_005622181.1
Bos taurus (cattle)	CLCA3	NP_851361.1
Mus musculus (house mouse)	Clca2	NP_085104.1
Rattus norvegicus (Norway rat)	Clca2	NP_001013220.1
Xenopus tropicalis (tropical clawed frog)	clca1	NP_001184129.1
Xenopus tropicalis (tropical clawed frog)	LOC100490932	XP_002934201.1
	LOC715292	XP_001105446.1
Mus musculus (house mouse)	Clca1	NP_034029.2
Xenopus tropicalis (tropical clawed frog)	LOC100488301	XP_002934186.1
Gallus gallus (chicken)	LOC424523	XP_422360.3
Xenopus tropicalis (tropical clawed frog)	clca3p	XP_002934204.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa20290	NM_001305589.1 Latest version!	Danio rerio chloride channel accessory 1 (clca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clca1 ( NM_001305589.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001292518.1 Danio rerio chloride channel accessory 1 (clca1), mRNA.

Price & Availability↑

CloneID	ODa20290
Clone ID Related Accession (Same CDS sequence)	NM_001305589.1
Accession Version	NM_001305589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	calcium-activated chloride channel regulator 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH485006.1 and BX927244.11. On Mar 20, 2015 this sequence version replaced XM_002664564.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01000072.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA4476821, SAMN01765705 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGACTGGA GAACGGTGTT TGTCGTATTA TGGATGCTGC TGCCGTACCC TTCAACTGCA 
ATTAAACTAG ATGGTGGTGG CTATGTTGAC ATTACGATTG CAATTGGTGC AAAAGTCAAG 
CAGGATGATA CACTTATCGA TAAAATCAAG GAAATGGTCA CCGATGGGTC TTTTTATCTT 
TATCATGCTT TGGATAAAAA GGTCTATTTG AAAGACGCCA CAATACTAGT TCCATCCCAC 
TGGAGCTGTA AGAGTTGCAG CAAAGCGAGA ACAGAATCAT TTGAAAAAGC CAAAATAAAA 
ATTGACCATG CTAAATTGAT GGAACCAAGA ACTAAACTGT ACGGAGAATG TGGAAAAGGG 
GGAGAGTACA TTCATTTCAC TCCAGACTTT CTCTTAAATG ACTCTGCCAT TCAGATGTAT 
GGACCAAGAG GAAAGGTTTT TTTGCATGAA TGGGCTCATC TGAGATGGGG TGTTTATGAT 
GAATACAATG AAGAAAAACC CTTTTACCTC TCTAATGGCC GTGTTGAATA TACAAGATGT 
ACTACAAACA TTGAAGGTCA GATTTTTGAG GTTAATGGAG GATCTCCTCA ATCATGCCGC 
ATTAATCCAG AAACGTTTTT ACCTAGCTCG GATTGCAAGT TTTTTCCAAA CAAAGATCAA 
AACACTGACA GCTCTGTAAT GTTTTCACCA AGTCTGGAAG CCGTGACCAC CTTTTGTCGT 
GAAACAGAAC ACAACTATGA AGCCGAAACC CAGCAAAATA TAATATGTAA TAACAAAGCA 
ACATGGACGG TAATATTCGA GGATTCTGTA GATAAAGATG CACTGTCGTT TCTACCACCA 
CTGCCATCGA CTCCATCGCC GCCAATATTT AAACTTTTGC AGCGGAAGAA ACGGGCTGTT 
TGTCTCATCC TTGATGTCTC AGGAAGCATG GCAACAGAAT CTAGAATTCT TCGCATGAGG 
CAGGCGGCCA CACATTTACT GCGGAACTAT GTTGAGGAAC AGGCCAGTGT TGGAATTGTA 
AAATTCAGTA CTGCAGCTTC TATTGTGAGC TCATTGACTA TTATTGAGAG TGATGCCACA 
AGAGATCATC TCATTAACTT GTTGCCCGAA ACTCCAGGTG GATCCACAAA CATGTGTAAT 
GGCCTTCGTC TAGGCTTACA GGTACTTTCA GAAGACGACA TGGATGCAAT AGGAGATGAA 
ATAATTTTTC TGACAGATGG TCAGGCAACA GACGACGTTA CATTGTGTAT TCCGGATGCA 
ATAAACAGTG GTGCTATTAT ACACACAATA GCTTTAAGTG ACAGTGCACA TAATGCATTG 
CAAGAGATGG CAGACAAAAC TGGTGGAATA TTTTTCTATT CCAAAGATGA TTTTACCTCC 
AACCAATTAA TGGATGCATT TGCTTCACTT ACATTATCCA CTGGAGATCA TTCAAATGAA 
CCTGTTCAGC TTGAGAGTGT GGGAACCAGA ACATCTGATT GGTTCAATGG GACGGTATCA 
GTGGATCAGA CTGTTGGCAA CAAAACCAGC TTTGTGATCA TTTATGAACG AATTTTTCCC 
GCTGTTTACA TACAGTCACC GAGTGGCATT GTCTACACTG AGGCACAAAT GAATCATGAT 
AATCGGCTGA AAACAGTCAC TTTAAAAGTT GCTGAAACCG CAGAGCCTGG AGACTGGGCG 
TACAGTATCA AAACTTCAAC AGATCAGGCA TTTACAATAA CAGTAACAAG TCAAGCATCA 
CATGAGGATG TTCCTCCAAT CATTGTCAAA ACCCGCATGA ACCAGCAGGT CAGCGATGGG 
ACTAAACCTA TGACGGTGTT TGCTGAGGTC AGTCAGAATT ACAGGCCTGT GATAAATGTT 
GAAGTGTGGG CTACGCTGGA GCCTGCAACT GGGCCTATAC AAAGTTTACA ACTCCTGGAC 
AATGGAGCAG GAGCCGATGT TATGGCAAAT GATGGAATCT ATTCCAGATA TTTCACAAAA 
ATGGTAAACG GGAGATGCAG CTTGAAAGTG AGAGCGAAGA ATCAAGATGG AAAGGCCAGA 
TTTGCGGTCC AGAAAAGGAG TGGTGCCCCA TACGTACCTG GATATGTGGT AGATGGTGTT 
GTAGAGCTGA ACCCGCCAAA TCCTTCAGTT TCTGATGAAC CAATTGAGGT TGGAAGCTTC 
ACCAGAACAG CCACCGGTGA GAGTTTTGAG GTGACTCTAA CAGGCTCAAC ACCACCAAAT 
TTCCCTCCTA GCAAAGTGAC AGATCTAGGA GCTAAAATCC TTGAGGAGGC TGTGCTTCTC 
AGCTGGACGG CTCCTGGTGA AGACCTCGAC CAAGGAAGAG CTAAATCTTA CGAGATCAGA 
TGGAGCTTTG ACCTGGACAT GCTTCGGCAA GATTTCAGCA ATGGCCATGT AGTCGACACA 
GCAACTGTCT CACCTCAGGA GGCTGGATCA GTGGAACAGC ACGCATTCAA TCTCAGTTTA 
CCAATCCAAA ATGGCACCAC GCTCTTTTTT GCGGTTCAGA CTTTGGATGA ACACGATGCC 
AAATCTGATA CCTCCAACAT TGCTTCAGCA TCAATGATCC TCCCTAATCC AAAACCACCG 
GGAATATCAA ACCCAGGCTT GAATTTGACT GTTCTTGTCA GCTCTTTGTG TGCGGTAACT 
ATCGTCATAT GCATTATTGC TGGTGTAACC ACATGGGCAG TGAGACGAAG AAGACCTGCA 
TTAAGAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292518.1
CDS	14..2725
Translation	MDWRTVFVVLWMLLPYPSTAIKLDGGGYVDITIAIGAKVKQDDTLIDKIKEMVTDGSFYLYHALDKKVYLKDATILVPSHWSCKSCSKARTESFEKAKIKIDHAKLMEPRTKLYGECGKGGEYIHFTPDFLLNDSAIQMYGPRGKVFLHEWAHLRWGVYDEYNEEKPFYLSNGRVEYTRCTTNIEGQIFEVNGGSPQSCRINPETFLPSSDCKFFPNKDQNTDSSVMFSPSLEAVTTFCRETEHNYEAETQQNIICNNKATWTVIFEDSVDKDALSFLPPLPSTPSPPIFKLLQRKKRAVCLILDVSGSMATESRILRMRQAATHLLRNYVEEQASVGIVKFSTAASIVSSLTIIESDATRDHLINLLPETPGGSTNMCNGLRLGLQVLSEDDMDAIGDEIIFLTDGQATDDVTLCIPDAINSGAIIHTIALSDSAHNALQEMADKTGGIFFYSKDDFTSNQLMDAFASLTLSTGDHSNEPVQLESVGTRTSDWFNGTVSVDQTVGNKTSFVIIYERIFPAVYIQSPSGIVYTEAQMNHDNRLKTVTLKVAETAEPGDWAYSIKTSTDQAFTITVTSQASHEDVPPIIVKTRMNQQVSDGTKPMTVFAEVSQNYRPVINVEVWATLEPATGPIQSLQLLDNGAGADVMANDGIYSRYFTKMVNGRCSLKVRAKNQDGKARFAVQKRSGAPYVPGYVVDGVVELNPPNPSVSDEPIEVGSFTRTATGESFEVTLTGSTPPNFPPSKVTDLGAKILEEAVLLSWTAPGEDLDQGRAKSYEIRWSFDLDMLRQDFSNGHVVDTATVSPQEAGSVEQHAFNLSLPIQNGTTLFFAVQTLDEHDAKSDTSNIASASMILPNPKPPGISNPGLNLTVLVSSLCAVTIVICIIAGVTTWAVRRRRPALRI

Target ORF information:

RefSeq Version	NM_001305589.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chloride channel accessory 1 (clca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305589.1

ORF Insert Sequence:

ATGGACTGGA GAACGGTGTT TGTCGTATTA TGGATGCTGC TGCCGTACCC TTCAACTGCA 
ATTAAACTAG ATGGTGGTGG CTATGTTGAC ATTACGATTG CAATTGGTGC AAAAGTCAAG 
CAGGATGATA CACTTATCGA TAAAATCAAG GAAATGGTCA CCGATGGGTC TTTTTATCTT 
TATCATGCTT TGGATAAAAA GGTCTATTTG AAAGACGCCA CAATACTAGT TCCATCCCAC 
TGGAGCTGTA AGAGTTGCAG CAAAGCGAGA ACAGAATCAT TTGAAAAAGC CAAAATAAAA 
ATTGACCATG CTAAATTGAT GGAACCAAGA ACTAAACTGT ACGGAGAATG TGGAAAAGGG 
GGAGAGTACA TTCATTTCAC TCCAGACTTT CTCTTAAATG ACTCTGCCAT TCAGATGTAT 
GGACCAAGAG GAAAGGTTTT TTTGCATGAA TGGGCTCATC TGAGATGGGG TGTTTATGAT 
GAATACAATG AAGAAAAACC CTTTTACCTC TCTAATGGCC GTGTTGAATA TACAAGATGT 
ACTACAAACA TTGAAGGTCA GATTTTTGAG GTTAATGGAG GATCTCCTCA ATCATGCCGC 
ATTAATCCAG AAACGTTTTT ACCTAGCTCG GATTGCAAGT TTTTTCCAAA CAAAGATCAA 
AACACTGACA GCTCTGTAAT GTTTTCACCA AGTCTGGAAG CCGTGACCAC CTTTTGTCGT 
GAAACAGAAC ACAACTATGA AGCCGAAACC CAGCAAAATA TAATATGTAA TAACAAAGCA 
ACATGGACGG TAATATTCGA GGATTCTGTA GATAAAGATG CACTGTCGTT TCTACCACCA 
CTGCCATCGA CTCCATCGCC GCCAATATTT AAACTTTTGC AGCGGAAGAA ACGGGCTGTT 
TGTCTCATCC TTGATGTCTC AGGAAGCATG GCAACAGAAT CTAGAATTCT TCGCATGAGG 
CAGGCGGCCA CACATTTACT GCGGAACTAT GTTGAGGAAC AGGCCAGTGT TGGAATTGTA 
AAATTCAGTA CTGCAGCTTC TATTGTGAGC TCATTGACTA TTATTGAGAG TGATGCCACA 
AGAGATCATC TCATTAACTT GTTGCCCGAA ACTCCAGGTG GATCCACAAA CATGTGTAAT 
GGCCTTCGTC TAGGCTTACA GGTACTTTCA GAAGACGACA TGGATGCAAT AGGAGATGAA 
ATAATTTTTC TGACAGATGG TCAGGCAACA GACGACGTTA CATTGTGTAT TCCGGATGCA 
ATAAACAGTG GTGCTATTAT ACACACAATA GCTTTAAGTG ACAGTGCACA TAATGCATTG 
CAAGAGATGG CAGACAAAAC TGGTGGAATA TTTTTCTATT CCAAAGATGA TTTTACCTCC 
AACCAATTAA TGGATGCATT TGCTTCACTT ACATTATCCA CTGGAGATCA TTCAAATGAA 
CCTGTTCAGC TTGAGAGTGT GGGAACCAGA ACATCTGATT GGTTCAATGG GACGGTATCA 
GTGGATCAGA CTGTTGGCAA CAAAACCAGC TTTGTGATCA TTTATGAACG AATTTTTCCC 
GCTGTTTACA TACAGTCACC GAGTGGCATT GTCTACACTG AGGCACAAAT GAATCATGAT 
AATCGGCTGA AAACAGTCAC TTTAAAAGTT GCTGAAACCG CAGAGCCTGG AGACTGGGCG 
TACAGTATCA AAACTTCAAC AGATCAGGCA TTTACAATAA CAGTAACAAG TCAAGCATCA 
CATGAGGATG TTCCTCCAAT CATTGTCAAA ACCCGCATGA ACCAGCAGGT CAGCGATGGG 
ACTAAACCTA TGACGGTGTT TGCTGAGGTC AGTCAGAATT ACAGGCCTGT GATAAATGTT 
GAAGTGTGGG CTACGCTGGA GCCTGCAACT GGGCCTATAC AAAGTTTACA ACTCCTGGAC 
AATGGAGCAG GAGCCGATGT TATGGCAAAT GATGGAATCT ATTCCAGATA TTTCACAAAA 
ATGGTAAACG GGAGATGCAG CTTGAAAGTG AGAGCGAAGA ATCAAGATGG AAAGGCCAGA 
TTTGCGGTCC AGAAAAGGAG TGGTGCCCCA TACGTACCTG GATATGTGGT AGATGGTGTT 
GTAGAGCTGA ACCCGCCAAA TCCTTCAGTT TCTGATGAAC CAATTGAGGT TGGAAGCTTC 
ACCAGAACAG CCACCGGTGA GAGTTTTGAG GTGACTCTAA CAGGCTCAAC ACCACCAAAT 
TTCCCTCCTA GCAAAGTGAC AGATCTAGGA GCTAAAATCC TTGAGGAGGC TGTGCTTCTC 
AGCTGGACGG CTCCTGGTGA AGACCTCGAC CAAGGAAGAG CTAAATCTTA CGAGATCAGA 
TGGAGCTTTG ACCTGGACAT GCTTCGGCAA GATTTCAGCA ATGGCCATGT AGTCGACACA 
GCAACTGTCT CACCTCAGGA GGCTGGATCA GTGGAACAGC ACGCATTCAA TCTCAGTTTA 
CCAATCCAAA ATGGCACCAC GCTCTTTTTT GCGGTTCAGA CTTTGGATGA ACACGATGCC 
AAATCTGATA CCTCCAACAT TGCTTCAGCA TCAATGATCC TCCCTAATCC AAAACCACCG 
GGAATATCAA ACCCAGGCTT GAATTTGACT GTTCTTGTCA GCTCTTTGTG TGCGGTAACT 
ATCGTCATAT GCATTATTGC TGGTGTAACC ACATGGGCAG TGAGACGAAG AAGACCTGCA 
TTAAGAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M