arhgef10 cDNA ORF clone, Danio rerio(zebrafish)

The following arhgef10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145918 XM_009293079.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa145919 XM_021473176.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa145920 XM_021473177.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa39979 XM_009293082.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa39976 XM_009293079.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa39976 XM_021467421.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa39978 XM_009293081.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa39978 XM_009293081.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145918
    Clone ID Related Accession (Same CDS sequence) XM_009293079.3
    Accession Version XM_009293079.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG
    AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAAGCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CTTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGCT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGGAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACA GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTACCGTT GGATGAGCCA CTAAAGAACG TTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTCAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGGGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCAG AGCTGGAGGG AGCCACACTA AACCAGAACT GCAGCAGGGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291354.2
    CDS202..4440
    Translation

    Target ORF information:

    RefSeq Version XM_009293079.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293079.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG
    AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAAGCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CTTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGCT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGGAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACA GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTACCGTT GGATGAGCCA CTAAAGAACG TTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTCAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGGGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCAG AGCTGGAGGG AGCCACACTA AACCAGAACT GCAGCAGGGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145919
    Clone ID Related Accession (Same CDS sequence) XM_021473176.1
    Accession Version XM_021473176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG
    AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGTATG AGAAGCCTCT GGCAGAAATT GAGCCTCGGT TACTCAGTGA CCGCAAGCGT
    AAGGTGGTGT TTCATCGCAT TCGGGAGATC CTGCAGTGTC ATGCACTCTT CCAGATGGCG
    CTTGCCAGCA GAGTATCTGA CTGGGATGAG CTGGAGATGA TTGGAGATGT GTTTGTAGCA
    TCGTTTTCCA AGTCTATGGT GTTGGACGCT TACAGTGAAT ATGTGAATAA CTTCAGCTTG
    GCGATGGGAG TGGTGCGGAA GGCATGCGCC GCTAAACCCT GTTTCCTGGA CTTCCTCAAG
    CACCGCCAGG AGACAAGTAA AGACCGTCTG ACTCTTTATG GACTGATGAT GAAGCCTATT
    CAGCGGTTCC CTCAGTTTAT TCTGCTGCTG CAAGACATGT TAAAGAACAC GCCAGTGGGC
    CATGCTGATC GCCTTCCCCT TCAGATGGCT CTTACTGAAC TGGAAGTGCT TGCCGAAAAA
    TTAAACGAAA GGAAAAAGGA GGCTGACCAG CGCTGCGAGA TACGACACAT CGCTAAAGCT
    ATGAACGAAC ATTACCTCAA CAAGCTGCTG AGCAGCGGCA GCCGTTATCT GATTCGGTCT
    GATGACATGG TGGAAACCGT TTATAATGAT CGAGGGGAGA TCATTAAGAC TAAAGAACGG
    CGAGTCTTTC TCCTGAACGA TGTGCTCATG TGTGCCACCC CCAACCGGCG GCCATCTCAG
    GATGGTGTGA GTGTTGGAGC TGGAGGTGAA GGGGTTCCTT CAGGACAGCG TTTCTTGCTC
    AAGTGGAGTG TACCGTTAAG TTTTGTGGAG GTCGTGGAGT TTGGCTCCAG CGAGGAGATG
    GTTGACTCCC GGTTTCCACC CTCACACTCT GGAGATAAAG TTGTCATTAA TGCAAAACCT
    AATAAGTTAT ATATGGGGCC GGGACAGCTG TATCAGGACC TGCAGAACCT GATCCATGAC
    CTGGGCTTGG TGAACCAAAT TTCCACCATG ATCAGCAGTC TGAAAGGGAA TTATCAGAAT
    GTTAATGGGA CTGTTGCTCA GGACTGGGTT TCAGGTCTTC AGCGTCTCAT TTTAAAGAAA
    GAGGAGGAGA TTCGGGCAGC TGATCGCTGT AGGATACAAC TACAGTTACC AGGGAAACAA
    GACAAATACG GTCGACCCAC GTTTTTCACT GCAGTCTTCA ACACATTCAG TCCATCCATC
    AAGCAAACAT GGATCAGTAA TCTGCAGATG GCTAAACTGG CTTTACAGGA GGACAACATC
    CAAGGCTGGT TTTGTGCTGA AGATGATGGT AATCAGATGA GGAAGCAGAA TCATCCTCTC
    TTGCTCAAGC AAATGGCAGT GGTCATGTCT AAACTACAGG AGTTTAAGGT GGAGTGTGCC
    GTTCACAACC CCGAGCCTCA TGTGAACACC GACAGCACCG CAGATCTGCC TGTTATGGGT
    CACGGATGTG TGTGGGTGGC GAGTTGCACC AATCACATGG GTCAGGTGTC CATTGTGGCA
    CTGCAAAACT CAAATCCTAA AGTGACAGAG TGTTTTAATG TCGAGTCGCG TATTCTCTGT
    ATGACGCATG TACCGTTGGA TGAGCCACTA AAGAACGTTG AGGGAAAACC TGAGAATCGG
    AATGCCTGTG ATATGCCAAC TGTCTGTTTG GGAATGGAGG AAGGCAGTAT ATCTGTTTAT
    AAGAGCAGCC AGCGCTCTAA GAAAGTTCGC CTGCAGCACT TCTTCTCTCC TGATAAGTCT
    AGCGTCACGT GTTTGGTGTA CACGAGTGGG TGTCTGTATG CAGGTCTAGT CAGTGGTTCT
    CTGGTCATCT ACTCCAGAAC TGATGACGGC TTCTGGGTCG AGTCGGGGGC CTGTGTGTTG
    AAGCTGGGGG TGTTGCCGGT TAAAGCTCTA CTGGTGGTGG GCGAACACAT CTGGGCTTCA
    TCAGGAGGAC AGATCTTCAT CATCAGCACA CAGACAAACA CGGTGGAGCG TCAGTTTGAA
    GCCCATCAGG AAGAAGGAAT GATCGTGTCC CACATGGTGG TGGCTGGAGT GGGTATCTGG
    ATATCCTTCA GCACCGGCTC AACCCTTCGA CTCTTCCACA CCGAGACTCT CGACCACCTG
    CAAGACATCA ACATCGCCAC AGCTGTCAAT AACATACTCC CAGGACAGCA GAGAGTCTCT
    GTGAGCAGTC TGTTGGTGTG TCACGGTCTG CTGCTAGTGG GCACTAATCT GGGGGTCACG
    GTGGCCCTTT CTGTGCCCAG ACTACAGGGC ATCCCAAAGG TCACAGGTCG AGGAATGGTC
    TCTTTTCATG CACACCACGG CCCAGTCAAG TTCCTCACCA TGGCAACAGC AGTGTGTAAC
    GTCTCTCAAG CTACCATGGC AACCCTCACC TCCAGTACTC CCAGTCAGTG CCAGCCTGAG
    GCCTCAGAGC TGGAGGGAGC CACACTAAAC CAGAACTGCA GCAGGGGCTC ACATGCGCCT
    GTTCTCCAGG ACTCCCCGTC CTCCTCGTGT GGCTCTCTGG CTCTGTCTCA GGCATCTTTA
    GAGCACGGCC CAGAGGACGG GGCCATATAC GACCTGCTGA ACGAGCCGGC ACACTTCCAG
    AAGGCCAAGC AGACTGACAG AGTCAGTGCC AGCTCTGTGC TGGTGGTGTC AGGAGGGCTC
    GGACACCGCA GAATCAACCG AAAAACCAAA CAGTCCAAGC ACGAGGAGAT GGCGTCCACT
    ATCATGGTGT GGCAAATACC ACTGCCAAGC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328851.1
    CDS202..4437
    Translation

    Target ORF information:

    RefSeq Version XM_021473176.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG
    AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGTATG AGAAGCCTCT GGCAGAAATT GAGCCTCGGT TACTCAGTGA CCGCAAGCGT
    AAGGTGGTGT TTCATCGCAT TCGGGAGATC CTGCAGTGTC ATGCACTCTT CCAGATGGCG
    CTTGCCAGCA GAGTATCTGA CTGGGATGAG CTGGAGATGA TTGGAGATGT GTTTGTAGCA
    TCGTTTTCCA AGTCTATGGT GTTGGACGCT TACAGTGAAT ATGTGAATAA CTTCAGCTTG
    GCGATGGGAG TGGTGCGGAA GGCATGCGCC GCTAAACCCT GTTTCCTGGA CTTCCTCAAG
    CACCGCCAGG AGACAAGTAA AGACCGTCTG ACTCTTTATG GACTGATGAT GAAGCCTATT
    CAGCGGTTCC CTCAGTTTAT TCTGCTGCTG CAAGACATGT TAAAGAACAC GCCAGTGGGC
    CATGCTGATC GCCTTCCCCT TCAGATGGCT CTTACTGAAC TGGAAGTGCT TGCCGAAAAA
    TTAAACGAAA GGAAAAAGGA GGCTGACCAG CGCTGCGAGA TACGACACAT CGCTAAAGCT
    ATGAACGAAC ATTACCTCAA CAAGCTGCTG AGCAGCGGCA GCCGTTATCT GATTCGGTCT
    GATGACATGG TGGAAACCGT TTATAATGAT CGAGGGGAGA TCATTAAGAC TAAAGAACGG
    CGAGTCTTTC TCCTGAACGA TGTGCTCATG TGTGCCACCC CCAACCGGCG GCCATCTCAG
    GATGGTGTGA GTGTTGGAGC TGGAGGTGAA GGGGTTCCTT CAGGACAGCG TTTCTTGCTC
    AAGTGGAGTG TACCGTTAAG TTTTGTGGAG GTCGTGGAGT TTGGCTCCAG CGAGGAGATG
    GTTGACTCCC GGTTTCCACC CTCACACTCT GGAGATAAAG TTGTCATTAA TGCAAAACCT
    AATAAGTTAT ATATGGGGCC GGGACAGCTG TATCAGGACC TGCAGAACCT GATCCATGAC
    CTGGGCTTGG TGAACCAAAT TTCCACCATG ATCAGCAGTC TGAAAGGGAA TTATCAGAAT
    GTTAATGGGA CTGTTGCTCA GGACTGGGTT TCAGGTCTTC AGCGTCTCAT TTTAAAGAAA
    GAGGAGGAGA TTCGGGCAGC TGATCGCTGT AGGATACAAC TACAGTTACC AGGGAAACAA
    GACAAATACG GTCGACCCAC GTTTTTCACT GCAGTCTTCA ACACATTCAG TCCATCCATC
    AAGCAAACAT GGATCAGTAA TCTGCAGATG GCTAAACTGG CTTTACAGGA GGACAACATC
    CAAGGCTGGT TTTGTGCTGA AGATGATGGT AATCAGATGA GGAAGCAGAA TCATCCTCTC
    TTGCTCAAGC AAATGGCAGT GGTCATGTCT AAACTACAGG AGTTTAAGGT GGAGTGTGCC
    GTTCACAACC CCGAGCCTCA TGTGAACACC GACAGCACCG CAGATCTGCC TGTTATGGGT
    CACGGATGTG TGTGGGTGGC GAGTTGCACC AATCACATGG GTCAGGTGTC CATTGTGGCA
    CTGCAAAACT CAAATCCTAA AGTGACAGAG TGTTTTAATG TCGAGTCGCG TATTCTCTGT
    ATGACGCATG TACCGTTGGA TGAGCCACTA AAGAACGTTG AGGGAAAACC TGAGAATCGG
    AATGCCTGTG ATATGCCAAC TGTCTGTTTG GGAATGGAGG AAGGCAGTAT ATCTGTTTAT
    AAGAGCAGCC AGCGCTCTAA GAAAGTTCGC CTGCAGCACT TCTTCTCTCC TGATAAGTCT
    AGCGTCACGT GTTTGGTGTA CACGAGTGGG TGTCTGTATG CAGGTCTAGT CAGTGGTTCT
    CTGGTCATCT ACTCCAGAAC TGATGACGGC TTCTGGGTCG AGTCGGGGGC CTGTGTGTTG
    AAGCTGGGGG TGTTGCCGGT TAAAGCTCTA CTGGTGGTGG GCGAACACAT CTGGGCTTCA
    TCAGGAGGAC AGATCTTCAT CATCAGCACA CAGACAAACA CGGTGGAGCG TCAGTTTGAA
    GCCCATCAGG AAGAAGGAAT GATCGTGTCC CACATGGTGG TGGCTGGAGT GGGTATCTGG
    ATATCCTTCA GCACCGGCTC AACCCTTCGA CTCTTCCACA CCGAGACTCT CGACCACCTG
    CAAGACATCA ACATCGCCAC AGCTGTCAAT AACATACTCC CAGGACAGCA GAGAGTCTCT
    GTGAGCAGTC TGTTGGTGTG TCACGGTCTG CTGCTAGTGG GCACTAATCT GGGGGTCACG
    GTGGCCCTTT CTGTGCCCAG ACTACAGGGC ATCCCAAAGG TCACAGGTCG AGGAATGGTC
    TCTTTTCATG CACACCACGG CCCAGTCAAG TTCCTCACCA TGGCAACAGC AGTGTGTAAC
    GTCTCTCAAG CTACCATGGC AACCCTCACC TCCAGTACTC CCAGTCAGTG CCAGCCTGAG
    GCCTCAGAGC TGGAGGGAGC CACACTAAAC CAGAACTGCA GCAGGGGCTC ACATGCGCCT
    GTTCTCCAGG ACTCCCCGTC CTCCTCGTGT GGCTCTCTGG CTCTGTCTCA GGCATCTTTA
    GAGCACGGCC CAGAGGACGG GGCCATATAC GACCTGCTGA ACGAGCCGGC ACACTTCCAG
    AAGGCCAAGC AGACTGACAG AGTCAGTGCC AGCTCTGTGC TGGTGGTGTC AGGAGGGCTC
    GGACACCGCA GAATCAACCG AAAAACCAAA CAGTCCAAGC ACGAGGAGAT GGCGTCCACT
    ATCATGGTGT GGCAAATACC ACTGCCAAGC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145920
    Clone ID Related Accession (Same CDS sequence) XM_021473177.1
    Accession Version XM_021473177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCGTTCATCC GCACCAGATC TCTCTGTCAT
    GAGAAGAAAC CCAGCTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA GCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCTTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GCTGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGGAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACAGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTACC GTTGGATGAG CCACTAAAGA ACGTTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTCAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGGGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CAGAGCTGGA GGGAGCCACA CTAAACCAGA ACTGCAGCAG GGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328852.1
    CDS202..4323
    Translation

    Target ORF information:

    RefSeq Version XM_021473177.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCGTTCATCC GCACCAGATC TCTCTGTCAT
    GAGAAGAAAC CCAGCTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA GCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCTTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GCTGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGGAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACAGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTACC GTTGGATGAG CCACTAAAGA ACGTTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTCAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGGGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CAGAGCTGGA GGGAGCCACA CTAAACCAGA ACTGCAGCAG GGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39979
    Clone ID Related Accession (Same CDS sequence) XM_009293082.2
    Accession Version XM_009293082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGTAACTG TTGTTGGAGC AGTAAATACT CCTGTGGACA GTGTTTTTAG TGAAGAGTGT
    CCGGTCATCA AAGAGGAAGA CGCTCTTACT AAGTGGGTTT CAGACCCAGC AAACACAGCC
    TGGATGGAGA GTCCAGATGA AGTGATTTAT GATGATGTAC CAAGAGAAAA CTCTGGATCC
    ACAACAGACC CAGATGAGAT GATCTATGAT GACGTTGAGT TCGGGGAGGA GGGTGGCAGC
    AGCTCGCTGG ACAATGGCTG GAGCTCCAGC GAATTTGAGA GCTATGAGGA AAACAGTGAC
    GTAGAGAACG GACATGGAGA AAACGGCCTC CCGGACGCCT TCGTCAGGGG CCGTGCACCC
    AGCAAGAAAA CACATCTGTC TGAAGATCTG GCACGTTTGA AAGAGCACTA TGAGAGAAAG
    ATGCGAGATC TCATGGCAAA CACAGTGGGA ACAGTAGAGC TACAGCAGCT CAAACACAAA
    CACGAGCAGA AGATGCAGCG GCTGGTAAAA GCTGCCAAAG ATGGAACTAA AGACGGCTTG
    CAGAAAACCA AAGCCGCAGT GAGAAAGGGC CGATCATTCA TCCGCACCAG ATCTCTCTGT
    CATGATAAGA AACCCAACTG CTTTGATGAA GAGTCAGATC TATTTATTGA GGTGGAGTGT
    TTTAATGTGG ACCCAGTGCT TGGACCTGCG CCTGACGGAC TCACTCAACA CCAGGTAATG
    AGGAGATGCA TTCTGGAGAA GATTCTGGAG AGCGAGAAGA GCTATTTGGA AGCTCTGAAG
    AGAATTTTAG AGCAGTATGA GAATCCTCTG GCAGAAATTG AGCCTCGGTT ACTCAGTGAC
    CGCAAGCGTA AGGTGGTGTT TCATCGCATT CGGGAGATCC TGCAGTGTCA TGCACTCTTC
    CAGATGGCGC TTGCCAGCAG AGTATCTGAC TGGGATGAGC TGGAGATGAT TGGAGATGTG
    TTTGTAGCAT CGTTTTCCAA GTCTATGGTG TTGGACGCTT ACAGTGAATA TGTGAATAAC
    TTCAGCTTGG CGATGGGAGT GGTGCGGAAG GCATGCGCCG CTAAACCCTG TTTCCTGGAC
    TTCCTCAAGC ACCGCCAGGA GACAAGTAAA GACCGTCTGA CTCTTTATGG ACTGATGATG
    AAGCCTATTC AGCGGTTCCC TCAGTTTATT CTGCTGCTGC AAGACATGTT AAAGAACACG
    CCAGTGGGCC ATGCTGATCG CCTTCCCCTT CAGATGGCTC TTACTGAACT GGAAGTGCTT
    GCCGAAAAAT TAAACGAAAG GAAAAAGGAG GCTGACCAGC GCTGCGAGAT ACGACACATC
    GCTAAAGCTA TGAACGAACA TTACCTCAAC AAGCTGCTGA GCAGCGGCAG CCGTTATCTG
    ATTCGGTCTG ATGACATGGT GGAAACCGTT TATAATGATC GAGGGGAGAT CATTAAGACT
    AAAGAACGGC GAGTCTTTCT CCTGAACGAT GTGCTCATGT GTGCCACCCC CAACCGGCGG
    CCATCTCAGG ATGGTGTGAG TGTTGGAGCT GGAGGTGAAG GGGTTCCCTC AGGACAGCGT
    TTCTTGCTCA AGTGGAGTGT ACCGTTAAGT TTTGTGGAGG TCGTGGAGTT TGGCTCCAGC
    GAGGAGATGG TTGACTCCCG GTTTCCACCC TCACACTCTG GAGATAAAGT TGTCATTAAT
    GCAAAACCTA ATAAGTTATA TATGGGGCCG GGACAGCTGT ATCAGGACCT GCAGAACCTG
    ATCCATGACC TGGGCTTGGT GAACCAAATT TCCACCATGA TCAGCAGTCT GAAAGGGAAT
    TATCAGAATG TTAATGGGAC TGTTGCTCAG GACTGGGTTT CAGGTCTTCA GCGTCTCATT
    TTAAAGAAAG AGGAGGAGAT TCGGGCAGCT GATCGCTGTA GGATACAACT ACAGTTACCA
    GGGAAACAAG ACAAATACGG TCGACCCACG TTTTTCACTG CAGTCTTCAA CACATTCAGT
    CCATCCATCA AGCAAACATG GATCAGTAAT CTGCAGATGG CTAAACTGGC TTTACAGGAG
    GACAACATCC AAGGATGGTT TTGTGCTGAA GATGATGGTA ATCAGATGAG AAAGCAGAAT
    CATCCTCTCT TGCTCAAGCA AATGGCAGTG GTCATGTCTA AACTACAGGA GTTTAAGGTG
    GAGTGTGCCG TTCACAACCC CGAGCCTCAT GTGAACACCG ACAGCACCGC AGATCTGCCT
    GTTATGGGTC ACGGATGTGT GTGGGTGGCG AGTTGCACCA ATCACATGGG TCAGGTGTCC
    ATTGTGGCAC TGCAAAACTC AAATCCTAAA GTGACGGAGT GTTTTAATGT CGAGTCGCGT
    ATTCTCTGTA TGACGCATGT TCCGTTGGAT GAGCCACTAA AGAACGGTGA GGGAAAACCT
    GAGAATCGGA ATGCCTGTGA TATGCCAACT GTCTGTTTGG GAATGGAGGA AGGCAGTATA
    TCTGTTTATA AGAGCAGCCA GCGCTCTAAG AAAGTTCGCC TGCAGCACTT CTTCTCTCCT
    GATAAGTCTA GCGTCACGTG TTTGGTGTAC ACGAGTGGGT GTCTGTATGC AGGTCTAGTT
    AGTGGTTCTC TGGTCATCTA CTCCAGAACT GATGACGGCT TCTGGGTCGA GTCGGGGGCC
    TGTGTGTTGA AGCTGGGGGT GTTGCCGGTT AAAGCTCTAC TGGTGGTGAG CGAACACATC
    TGGGCTTCAT CAGGAGGACA GATCTTCATC ATCAGCACAC AGACAAACAC GGTGGAGCGT
    CAGTTTGAAG CCCATCAGGA AGAAGGAATG ATCGTGTCCC ACATGGTGGT GGCTGGAGTG
    GGTATCTGGA TATCCTTCAG CACCGGCTCA ACCCTTCGAC TCTTCCACAC CGAGACTCTC
    GACCACCTGC AAGACATCAA CATCGCCACA GCTGTCAATA ACATACTCCC AGGACAGCAG
    AGAGTCTCTG TGAGCAGTCT GTTGGTGTGT CACGGTCTGC TGCTAGTGGG CACTAATCTG
    GGGGTCACGG TGGCCCTTTC TGTGCCCAGA CTACAGGGCA TCCCAAAGGT CACAGGTCGA
    GGAATGGTCT CTTTTCATGC ACACCACGGC CCAGTCAAGT TCCTCACCAT GGCAACAGCA
    GTGTGTAACG TCTCTCAAGC TACCATGGCA ACCCTCACCT CCAGTACTCC CAGTCAGTGC
    CAGCCTGAGG CCTCGGAACT GGAGGTAGCC ACACTAAACC AGAACTGCAG CAGCGGCTCA
    CATGCGCCTG TTCTCCAGGA CTCCCCGTCC TCCTCGTGTG GCTCTCTGGC TCTGTCTCAG
    GCATCTTTAG AGCACGGCCC AGAGGACGGG GCCATATACG ACCTGCTGAA CGAGCCGGCA
    CACTTCCAGA AGGCCAAGCA GACTGACAGA GTCAGTGCCA GCTCTGTGCT GGTGGTGTCA
    GGAGGGCTCG GACACCGCAG AATCAACCGA AAAACCAAAC AGTCCAAGCA CGAGGAGATG
    GCGTCCACTA TCATGGTGTG GCAAATACCA CTGCCAAGCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291357.1
    CDS199..4143
    Translation

    Target ORF information:

    RefSeq Version XM_009293082.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGTAACTG TTGTTGGAGC AGTAAATACT CCTGTGGACA GTGTTTTTAG TGAAGAGTGT
    CCGGTCATCA AAGAGGAAGA CGCTCTTACT AAGTGGGTTT CAGACCCAGC AAACACAGCC
    TGGATGGAGA GTCCAGATGA AGTGATTTAT GATGATGTAC CAAGAGAAAA CTCTGGATCC
    ACAACAGACC CAGATGAGAT GATCTATGAT GACGTTGAGT TCGGGGAGGA GGGTGGCAGC
    AGCTCGCTGG ACAATGGCTG GAGCTCCAGC GAATTTGAGA GCTATGAGGA AAACAGTGAC
    GTAGAGAACG GACATGGAGA AAACGGCCTC CCGGACGCCT TCGTCAGGGG CCGTGCACCC
    AGCAAGAAAA CACATCTGTC TGAAGATCTG GCACGTTTGA AAGAGCACTA TGAGAGAAAG
    ATGCGAGATC TCATGGCAAA CACAGTGGGA ACAGTAGAGC TACAGCAGCT CAAACACAAA
    CACGAGCAGA AGATGCAGCG GCTGGTAAAA GCTGCCAAAG ATGGAACTAA AGACGGCTTG
    CAGAAAACCA AAGCCGCAGT GAGAAAGGGC CGATCATTCA TCCGCACCAG ATCTCTCTGT
    CATGATAAGA AACCCAACTG CTTTGATGAA GAGTCAGATC TATTTATTGA GGTGGAGTGT
    TTTAATGTGG ACCCAGTGCT TGGACCTGCG CCTGACGGAC TCACTCAACA CCAGGTAATG
    AGGAGATGCA TTCTGGAGAA GATTCTGGAG AGCGAGAAGA GCTATTTGGA AGCTCTGAAG
    AGAATTTTAG AGCAGTATGA GAATCCTCTG GCAGAAATTG AGCCTCGGTT ACTCAGTGAC
    CGCAAGCGTA AGGTGGTGTT TCATCGCATT CGGGAGATCC TGCAGTGTCA TGCACTCTTC
    CAGATGGCGC TTGCCAGCAG AGTATCTGAC TGGGATGAGC TGGAGATGAT TGGAGATGTG
    TTTGTAGCAT CGTTTTCCAA GTCTATGGTG TTGGACGCTT ACAGTGAATA TGTGAATAAC
    TTCAGCTTGG CGATGGGAGT GGTGCGGAAG GCATGCGCCG CTAAACCCTG TTTCCTGGAC
    TTCCTCAAGC ACCGCCAGGA GACAAGTAAA GACCGTCTGA CTCTTTATGG ACTGATGATG
    AAGCCTATTC AGCGGTTCCC TCAGTTTATT CTGCTGCTGC AAGACATGTT AAAGAACACG
    CCAGTGGGCC ATGCTGATCG CCTTCCCCTT CAGATGGCTC TTACTGAACT GGAAGTGCTT
    GCCGAAAAAT TAAACGAAAG GAAAAAGGAG GCTGACCAGC GCTGCGAGAT ACGACACATC
    GCTAAAGCTA TGAACGAACA TTACCTCAAC AAGCTGCTGA GCAGCGGCAG CCGTTATCTG
    ATTCGGTCTG ATGACATGGT GGAAACCGTT TATAATGATC GAGGGGAGAT CATTAAGACT
    AAAGAACGGC GAGTCTTTCT CCTGAACGAT GTGCTCATGT GTGCCACCCC CAACCGGCGG
    CCATCTCAGG ATGGTGTGAG TGTTGGAGCT GGAGGTGAAG GGGTTCCCTC AGGACAGCGT
    TTCTTGCTCA AGTGGAGTGT ACCGTTAAGT TTTGTGGAGG TCGTGGAGTT TGGCTCCAGC
    GAGGAGATGG TTGACTCCCG GTTTCCACCC TCACACTCTG GAGATAAAGT TGTCATTAAT
    GCAAAACCTA ATAAGTTATA TATGGGGCCG GGACAGCTGT ATCAGGACCT GCAGAACCTG
    ATCCATGACC TGGGCTTGGT GAACCAAATT TCCACCATGA TCAGCAGTCT GAAAGGGAAT
    TATCAGAATG TTAATGGGAC TGTTGCTCAG GACTGGGTTT CAGGTCTTCA GCGTCTCATT
    TTAAAGAAAG AGGAGGAGAT TCGGGCAGCT GATCGCTGTA GGATACAACT ACAGTTACCA
    GGGAAACAAG ACAAATACGG TCGACCCACG TTTTTCACTG CAGTCTTCAA CACATTCAGT
    CCATCCATCA AGCAAACATG GATCAGTAAT CTGCAGATGG CTAAACTGGC TTTACAGGAG
    GACAACATCC AAGGATGGTT TTGTGCTGAA GATGATGGTA ATCAGATGAG AAAGCAGAAT
    CATCCTCTCT TGCTCAAGCA AATGGCAGTG GTCATGTCTA AACTACAGGA GTTTAAGGTG
    GAGTGTGCCG TTCACAACCC CGAGCCTCAT GTGAACACCG ACAGCACCGC AGATCTGCCT
    GTTATGGGTC ACGGATGTGT GTGGGTGGCG AGTTGCACCA ATCACATGGG TCAGGTGTCC
    ATTGTGGCAC TGCAAAACTC AAATCCTAAA GTGACGGAGT GTTTTAATGT CGAGTCGCGT
    ATTCTCTGTA TGACGCATGT TCCGTTGGAT GAGCCACTAA AGAACGGTGA GGGAAAACCT
    GAGAATCGGA ATGCCTGTGA TATGCCAACT GTCTGTTTGG GAATGGAGGA AGGCAGTATA
    TCTGTTTATA AGAGCAGCCA GCGCTCTAAG AAAGTTCGCC TGCAGCACTT CTTCTCTCCT
    GATAAGTCTA GCGTCACGTG TTTGGTGTAC ACGAGTGGGT GTCTGTATGC AGGTCTAGTT
    AGTGGTTCTC TGGTCATCTA CTCCAGAACT GATGACGGCT TCTGGGTCGA GTCGGGGGCC
    TGTGTGTTGA AGCTGGGGGT GTTGCCGGTT AAAGCTCTAC TGGTGGTGAG CGAACACATC
    TGGGCTTCAT CAGGAGGACA GATCTTCATC ATCAGCACAC AGACAAACAC GGTGGAGCGT
    CAGTTTGAAG CCCATCAGGA AGAAGGAATG ATCGTGTCCC ACATGGTGGT GGCTGGAGTG
    GGTATCTGGA TATCCTTCAG CACCGGCTCA ACCCTTCGAC TCTTCCACAC CGAGACTCTC
    GACCACCTGC AAGACATCAA CATCGCCACA GCTGTCAATA ACATACTCCC AGGACAGCAG
    AGAGTCTCTG TGAGCAGTCT GTTGGTGTGT CACGGTCTGC TGCTAGTGGG CACTAATCTG
    GGGGTCACGG TGGCCCTTTC TGTGCCCAGA CTACAGGGCA TCCCAAAGGT CACAGGTCGA
    GGAATGGTCT CTTTTCATGC ACACCACGGC CCAGTCAAGT TCCTCACCAT GGCAACAGCA
    GTGTGTAACG TCTCTCAAGC TACCATGGCA ACCCTCACCT CCAGTACTCC CAGTCAGTGC
    CAGCCTGAGG CCTCGGAACT GGAGGTAGCC ACACTAAACC AGAACTGCAG CAGCGGCTCA
    CATGCGCCTG TTCTCCAGGA CTCCCCGTCC TCCTCGTGTG GCTCTCTGGC TCTGTCTCAG
    GCATCTTTAG AGCACGGCCC AGAGGACGGG GCCATATACG ACCTGCTGAA CGAGCCGGCA
    CACTTCCAGA AGGCCAAGCA GACTGACAGA GTCAGTGCCA GCTCTGTGCT GGTGGTGTCA
    GGAGGGCTCG GACACCGCAG AATCAACCGA AAAACCAAAC AGTCCAAGCA CGAGGAGATG
    GCGTCCACTA TCATGGTGTG GCAAATACCA CTGCCAAGCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39976
    Clone ID Related Accession (Same CDS sequence) XM_021467421.1 , XM_009293079.2
    Accession Version XM_021467421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT
    AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323096.1
    CDS204..4442
    Translation

    Target ORF information:

    RefSeq Version XM_021467421.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT
    AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39976
    Clone ID Related Accession (Same CDS sequence) XM_021467421.1 , XM_009293079.2
    Accession Version XM_009293079.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT
    AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291354.1
    CDS200..4438
    Translation

    Target ORF information:

    RefSeq Version XM_009293079.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293079.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA
    GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG
    CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA
    ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT
    AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT
    GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA
    TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT
    TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG
    CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG
    GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA
    GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC
    TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC
    AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT
    ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG
    GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA
    AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA
    GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG
    TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA
    CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT
    CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG
    CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG
    ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA
    CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT
    GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG
    AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG
    AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA
    CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC
    ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC
    ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT
    CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT
    GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG
    GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG
    GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC
    TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT
    CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT
    TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG
    TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT
    TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG
    TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT
    TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT
    GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC
    TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC
    CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC
    TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC
    ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG
    GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT
    AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT
    GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG
    CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT
    TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC
    CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG
    CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC
    ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39978
    Clone ID Related Accession (Same CDS sequence) XM_009293081.2 , XM_009293081.3
    Accession Version XM_009293081.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT
    GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291356.1
    CDS200..4321
    Translation

    Target ORF information:

    RefSeq Version XM_009293081.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT
    GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39978
    Clone ID Related Accession (Same CDS sequence) XM_009293081.2 , XM_009293081.3
    Accession Version XM_009293081.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT
    GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291356.1
    CDS204..4325
    Translation

    Target ORF information:

    RefSeq Version XM_009293081.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293081.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
    GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG
    CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC
    GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG
    CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA
    CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA
    GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA
    GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT
    GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC
    TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG
    TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG
    ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA
    ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC
    ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG
    GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA
    GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG
    CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG
    AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG
    AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG
    AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT
    GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT
    AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG
    AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA
    ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC
    AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG
    ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT
    GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC
    AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC
    CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG
    CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA
    GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC
    GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT
    AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT
    CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA
    GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA
    TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC
    TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG
    GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA
    AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC
    CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT
    CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA
    AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG
    AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA
    TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC
    AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT
    CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG
    TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT
    ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT
    GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT
    CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG
    AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT
    GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT
    AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT
    GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT
    GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG
    GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG
    TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT
    ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC
    CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA
    GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG
    GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA
    ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG
    TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG
    CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT
    GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA
    TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC
    TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA
    GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG
    TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.