rasgef1ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rasgef1ba
Entrez Gene ID	327572
Full Name	RasGEF domain family, member 1Ba
Synonyms	rasgef1b,wu:fi18a09,zgc:66487
General protein information	Preferred Names RasGEF domain family, member 1Ba Names ras-GEF domain-containing family member 1B-A RasGEF domain family, member 1B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165278.4	XP_005165335.1	ras-GEF domain-containing family member 1B-A isoform X1
XM_005165280.3	XP_005165337.1	ras-GEF domain-containing family member 1B-A isoform X3
XM_005165280.4	XP_005165337.1	ras-GEF domain-containing family member 1B-A isoform X3
NM_199829.1	NP_956123.1	ras-GEF domain-containing family member 1B-A
XM_005165278.3	XP_005165335.1	ras-GEF domain-containing family member 1B-A isoform X1
XM_005165279.3	XP_005165336.1	ras-GEF domain-containing family member 1B-A isoform X2
XM_005165279.4	XP_005165336.1	ras-GEF domain-containing family member 1B-A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGEF1B	NP_689758.1
Pan troglodytes (chimpanzee)	RASGEF1B	XP_526570.3
Caenorhabditis elegans (roundworm)	R05G6.10	NP_501217.2
Canis lupus familiaris (dog)	RASGEF1B	XP_005639172.1
Bos taurus (cattle)	RASGEF1B	NP_001077118.1
Mus musculus (house mouse)	Rasgef1b	NP_665838.1
Gallus gallus (chicken)	RASGEF1B	XP_003641217.2
Danio rerio (zebrafish)	rasgef1ba	NP_956123.1
Rattus norvegicus (Norway rat)	Rasgef1b	XP_006250753.1
Drosophila melanogaster (fruit fly)	CG7369	NP_649415.2
Xenopus tropicalis (tropical clawed frog)	rasgef1b	NP_001016913.1

Gene Ontology
Bibliography

Related articles in PubMed

Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.
Dubrulle J, Jordan BM, Akhmetova L, Farrell JA, Kim SH, Solnica-Krezel L, Schier AF
eLife4(2015 Apr)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Quantitative genetic analysis of retinal degeneration in the blind cavefish Astyanax mexicanus.
O'Quin KE, Yoshizawa M, Doshi P, Jeffery WR
PloS one8(2)e57281(2013)

Evolution of follistatin in teleosts revealed through phylogenetic, genomic and expression analyses.
Macqueen DJ, Johnston IA
Development genes and evolution218(1)1-14(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study showa that three rasgef1b transcripts are generated from two transcriptional start sites and by alternative splicing.
Title: Expression of rasgef1b in zebrafish.

The following rasgef1ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasgef1ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38635	NM_199829.1 Latest version!	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa10675	XM_005165278.4 Latest version!	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa10676	XM_005165279.4 Latest version!	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa10677	XM_005165280.4 Latest version!	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa10677	XM_005165280.3	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa10675	XM_005165278.3	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa10676	XM_005165279.3	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rasgef1ba ( NM_199829.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956123.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), mRNA.

Price & Availability↑

CloneID	ODa38635
Clone ID Related Accession (Same CDS sequence)	NM_199829.1
Accession Version	NM_199829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	ras-GEF domain-containing family member 1B-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055678.1. ##Evidence-Data-START## Transcript exon combination :: BC055678.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAGGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATACA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT GTGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTCAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCAAAAGT TAAAACTGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG GACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AGAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956123.1
CDS	304..1725
Translation	MPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWGLAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	NM_199829.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199829.1

ORF Insert Sequence:

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAGGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATACA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT GTGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTCAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCAAAAGT TAAAACTGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG GACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AGAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165278.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165335.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10675
Clone ID Related Accession (Same CDS sequence)	XM_005165278.4 , XM_005165278.3
Accession Version	XM_005165278.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165278.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGCCC 
ACCATATTTG GGACACTTAT TGAAAAACCA CTTGGGGACC ACAATGCAGA CAAAATGCCT 
CAGACGCCAC CCTTTCCAGT GATGTATGGA TCCACCAATG GATACAACAA GAATCTCTAC 
AAGGCCAAGG AAGAGGACTA TGAAGGCTTG TATTACCATG ACAACAACCT GATATCTGGG 
TCCTTGGAAG CTCTTATTGA GCACTTGGTT CCTACAGTGG CGTACTATCC AGATAGGACA 
TATATCTTCA CATTCCTGCT CAGCTCGCGT CTCTTTCTTC ACCCATATGA GCTGATGTCC 
AAAGTGTGCC ATTTATGTGT GGACCAGCAG AGACTCAGTG ATCCTCAGGC AGACAAGGCT 
AGAGTCAGGA AAATTGCTCC CAAGATCCTG CAGTTGCTGA CTGAGTGGAC GGAGACGTTC 
CCCTATGACT TTAGGGATGA GAGGATGATG AAGAGCCTGA AGGAACTAAC ACATCGTCTG 
AGCACTGGAG AGGATCTGTA TCGTAAGGCG GTGCAACAGA TGACACAAAC GCTGATCCGT 
AAGCTCACCA CACTGAGTCA GTATGAAGAA GCGCTGGCCA AGATCAACTC CTCGGTGACG 
GACAGACTAA CCGTGCTGAA GACCAAACCG CAGTCCATCC AGAGAGACAT TCTGACCATC 
TGCAACGACC CCTTCACCCT GGCACAACAA CTCACACACA TTGAACTGGA AAGGCTAAGC 
TACATCGGAC CGGAGGAGTT TGTTCAGGCG TTTGTTCAGA AAGACCCTCT TGATAATGAC 
AAGAATTGCT TCAGTGATCA CAAGAAGGCA AGTAACCTGG AGGCTTACGT AGAGTGGTTC 
AACAGGCTCA GCTACCTGGT GGCGACGGAG ATCTGCATGC CTGTCAAGAA GAAACATCGG 
GCTCGAGTCA TCGAATTCTT TATCGACGTG GCACGAGAAT GCTTCAACAT TGGCAACTTC 
AACTCGCTCA TGGCCATCAT TACCGGGATG AACATGAGTC CTGTGTCGCG ACTCAAGAAG 
ACCTGGGCCA AAGTTAAAAC CGCCAAATTT GATATACTGG AGCACCAGAT GGATCCCTCC 
AGCAATTTCT ACAACTACAG GACGGCACTG CGCGGAGCCA CTCAGAGATC CATCACTGCT 
CACAGCAGCA GAGAGAAGAT TGTTATCCCT TTCTTCAGCC TCCTAATTAA AGACATTTAC 
TTCCTCAACG AAGGATGTGC CAACAGGCTC CCGAACGGAC ATGTGAATTT TGAGAAATTC 
TGGGAACTAG CCAAACAGGT GACAGAGTTC ATGACTTGGA AGAAGGTGGA GTGTCCTTTC 
GACAGAGACC GCAAGATTCT GCAGTACCTC CTGACCGCTC CAGTCTTCAG TGAAGATGCT 
TTGTATCTGG CGTCATACGA GAGCGAAGGG CCCGAAAACA ACATGGAGAA AGACCGATGG 
AAATCTCTCA GGTCATCTTT GCTGAACAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165335.1
CDS	257..1852
Translation	MAKFLAELLGCTLPEKGASPMFECRSQPHIGLRHVTKQRPTIFGTLIEKPLGDHNADKMPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165278.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165278.4

ORF Insert Sequence:

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGCCC 
ACCATATTTG GGACACTTAT TGAAAAACCA CTTGGGGACC ACAATGCAGA CAAAATGCCT 
CAGACGCCAC CCTTTCCAGT GATGTATGGA TCCACCAATG GATACAACAA GAATCTCTAC 
AAGGCCAAGG AAGAGGACTA TGAAGGCTTG TATTACCATG ACAACAACCT GATATCTGGG 
TCCTTGGAAG CTCTTATTGA GCACTTGGTT CCTACAGTGG CGTACTATCC AGATAGGACA 
TATATCTTCA CATTCCTGCT CAGCTCGCGT CTCTTTCTTC ACCCATATGA GCTGATGTCC 
AAAGTGTGCC ATTTATGTGT GGACCAGCAG AGACTCAGTG ATCCTCAGGC AGACAAGGCT 
AGAGTCAGGA AAATTGCTCC CAAGATCCTG CAGTTGCTGA CTGAGTGGAC GGAGACGTTC 
CCCTATGACT TTAGGGATGA GAGGATGATG AAGAGCCTGA AGGAACTAAC ACATCGTCTG 
AGCACTGGAG AGGATCTGTA TCGTAAGGCG GTGCAACAGA TGACACAAAC GCTGATCCGT 
AAGCTCACCA CACTGAGTCA GTATGAAGAA GCGCTGGCCA AGATCAACTC CTCGGTGACG 
GACAGACTAA CCGTGCTGAA GACCAAACCG CAGTCCATCC AGAGAGACAT TCTGACCATC 
TGCAACGACC CCTTCACCCT GGCACAACAA CTCACACACA TTGAACTGGA AAGGCTAAGC 
TACATCGGAC CGGAGGAGTT TGTTCAGGCG TTTGTTCAGA AAGACCCTCT TGATAATGAC 
AAGAATTGCT TCAGTGATCA CAAGAAGGCA AGTAACCTGG AGGCTTACGT AGAGTGGTTC 
AACAGGCTCA GCTACCTGGT GGCGACGGAG ATCTGCATGC CTGTCAAGAA GAAACATCGG 
GCTCGAGTCA TCGAATTCTT TATCGACGTG GCACGAGAAT GCTTCAACAT TGGCAACTTC 
AACTCGCTCA TGGCCATCAT TACCGGGATG AACATGAGTC CTGTGTCGCG ACTCAAGAAG 
ACCTGGGCCA AAGTTAAAAC CGCCAAATTT GATATACTGG AGCACCAGAT GGATCCCTCC 
AGCAATTTCT ACAACTACAG GACGGCACTG CGCGGAGCCA CTCAGAGATC CATCACTGCT 
CACAGCAGCA GAGAGAAGAT TGTTATCCCT TTCTTCAGCC TCCTAATTAA AGACATTTAC 
TTCCTCAACG AAGGATGTGC CAACAGGCTC CCGAACGGAC ATGTGAATTT TGAGAAATTC 
TGGGAACTAG CCAAACAGGT GACAGAGTTC ATGACTTGGA AGAAGGTGGA GTGTCCTTTC 
GACAGAGACC GCAAGATTCT GCAGTACCTC CTGACCGCTC CAGTCTTCAG TGAAGATGCT 
TTGTATCTGG CGTCATACGA GAGCGAAGGG CCCGAAAACA ACATGGAGAA AGACCGATGG 
AAATCTCTCA GGTCATCTTT GCTGAACAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165278.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165335.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10675
Clone ID Related Accession (Same CDS sequence)	XM_005165278.4 , XM_005165278.3
Accession Version	XM_005165278.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGCCC 
ACCATATTTG GGACACTTAT TGAAAAACCA CTTGGGGACC ACAATGCAGA CAAAATGCCT 
CAGACGCCAC CCTTTCCAGT GATGTATGGA TCCACCAATG GATACAACAA GAATCTCTAC 
AAGGCCAAGG AAGAGGACTA TGAAGGCTTG TATTACCATG ACAACAACCT GATATCTGGG 
TCCTTGGAAG CTCTTATTGA GCACTTGGTT CCTACAGTGG CGTACTATCC AGATAGGACA 
TATATCTTCA CATTCCTGCT CAGCTCGCGT CTCTTTCTTC ACCCATATGA GCTGATGTCC 
AAAGTGTGCC ATTTATGTGT GGACCAGCAG AGACTCAGTG ATCCTCAGGC AGACAAGGCT 
AGAGTCAGGA AAATTGCTCC CAAGATCCTG CAGTTGCTGA CTGAGTGGAC GGAGACGTTC 
CCCTATGACT TTAGGGATGA GAGGATGATG AAGAGCCTGA AGGAACTAAC ACATCGTCTG 
AGCACTGGAG AGGATCTGTA TCGTAAGGCG GTGCAACAGA TGACACAAAC GCTGATCCGT 
AAGCTCACCA CACTGAGTCA GTATGAAGAA GCGCTGGCCA AGATCAACTC CTCGGTGACG 
GACAGACTAA CCGTGCTGAA GACCAAACCG CAGTCCATCC AGAGAGACAT TCTGACCATC 
TGCAACGACC CCTTCACCCT GGCACAACAA CTCACACACA TTGAACTGGA AAGGCTAAGC 
TACATCGGAC CGGAGGAGTT TGTTCAGGCG TTTGTTCAGA AAGACCCTCT TGATAATGAC 
AAGAATTGCT TCAGTGATCA CAAGAAGGCA AGTAACCTGG AGGCTTACGT AGAGTGGTTC 
AACAGGCTCA GCTACCTGGT GGCGACGGAG ATCTGCATGC CTGTCAAGAA GAAACATCGG 
GCTCGAGTCA TCGAATTCTT TATCGACGTG GCACGAGAAT GCTTCAACAT TGGCAACTTC 
AACTCGCTCA TGGCCATCAT TACCGGGATG AACATGAGTC CTGTGTCGCG ACTCAAGAAG 
ACCTGGGCCA AAGTTAAAAC CGCCAAATTT GATATACTGG AGCACCAGAT GGATCCCTCC 
AGCAATTTCT ACAACTACAG GACGGCACTG CGCGGAGCCA CTCAGAGATC CATCACTGCT 
CACAGCAGCA GAGAGAAGAT TGTTATCCCT TTCTTCAGCC TCCTAATTAA AGACATTTAC 
TTCCTCAACG AAGGATGTGC CAACAGGCTC CCGAACGGAC ATGTGAATTT TGAGAAATTC 
TGGGAACTAG CCAAACAGGT GACAGAGTTC ATGACTTGGA AGAAGGTGGA GTGTCCTTTC 
GACAGAGACC GCAAGATTCT GCAGTACCTC CTGACCGCTC CAGTCTTCAG TGAAGATGCT 
TTGTATCTGG CGTCATACGA GAGCGAAGGG CCCGAAAACA ACATGGAGAA AGACCGATGG 
AAATCTCTCA GGTCATCTTT GCTGAACAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165335.1
CDS	261..1856
Translation	MAKFLAELLGCTLPEKGASPMFECRSQPHIGLRHVTKQRPTIFGTLIEKPLGDHNADKMPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165278.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165278.3

ORF Insert Sequence:

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGCCC 
ACCATATTTG GGACACTTAT TGAAAAACCA CTTGGGGACC ACAATGCAGA CAAAATGCCT 
CAGACGCCAC CCTTTCCAGT GATGTATGGA TCCACCAATG GATACAACAA GAATCTCTAC 
AAGGCCAAGG AAGAGGACTA TGAAGGCTTG TATTACCATG ACAACAACCT GATATCTGGG 
TCCTTGGAAG CTCTTATTGA GCACTTGGTT CCTACAGTGG CGTACTATCC AGATAGGACA 
TATATCTTCA CATTCCTGCT CAGCTCGCGT CTCTTTCTTC ACCCATATGA GCTGATGTCC 
AAAGTGTGCC ATTTATGTGT GGACCAGCAG AGACTCAGTG ATCCTCAGGC AGACAAGGCT 
AGAGTCAGGA AAATTGCTCC CAAGATCCTG CAGTTGCTGA CTGAGTGGAC GGAGACGTTC 
CCCTATGACT TTAGGGATGA GAGGATGATG AAGAGCCTGA AGGAACTAAC ACATCGTCTG 
AGCACTGGAG AGGATCTGTA TCGTAAGGCG GTGCAACAGA TGACACAAAC GCTGATCCGT 
AAGCTCACCA CACTGAGTCA GTATGAAGAA GCGCTGGCCA AGATCAACTC CTCGGTGACG 
GACAGACTAA CCGTGCTGAA GACCAAACCG CAGTCCATCC AGAGAGACAT TCTGACCATC 
TGCAACGACC CCTTCACCCT GGCACAACAA CTCACACACA TTGAACTGGA AAGGCTAAGC 
TACATCGGAC CGGAGGAGTT TGTTCAGGCG TTTGTTCAGA AAGACCCTCT TGATAATGAC 
AAGAATTGCT TCAGTGATCA CAAGAAGGCA AGTAACCTGG AGGCTTACGT AGAGTGGTTC 
AACAGGCTCA GCTACCTGGT GGCGACGGAG ATCTGCATGC CTGTCAAGAA GAAACATCGG 
GCTCGAGTCA TCGAATTCTT TATCGACGTG GCACGAGAAT GCTTCAACAT TGGCAACTTC 
AACTCGCTCA TGGCCATCAT TACCGGGATG AACATGAGTC CTGTGTCGCG ACTCAAGAAG 
ACCTGGGCCA AAGTTAAAAC CGCCAAATTT GATATACTGG AGCACCAGAT GGATCCCTCC 
AGCAATTTCT ACAACTACAG GACGGCACTG CGCGGAGCCA CTCAGAGATC CATCACTGCT 
CACAGCAGCA GAGAGAAGAT TGTTATCCCT TTCTTCAGCC TCCTAATTAA AGACATTTAC 
TTCCTCAACG AAGGATGTGC CAACAGGCTC CCGAACGGAC ATGTGAATTT TGAGAAATTC 
TGGGAACTAG CCAAACAGGT GACAGAGTTC ATGACTTGGA AGAAGGTGGA GTGTCCTTTC 
GACAGAGACC GCAAGATTCT GCAGTACCTC CTGACCGCTC CAGTCTTCAG TGAAGATGCT 
TTGTATCTGG CGTCATACGA GAGCGAAGGG CCCGAAAACA ACATGGAGAA AGACCGATGG 
AAATCTCTCA GGTCATCTTT GCTGAACAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165279.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165336.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10676
Clone ID Related Accession (Same CDS sequence)	XM_005165279.3 , XM_005165279.4
Accession Version	XM_005165279.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGGAC 
AAAATGCCTC AGACGCCACC CTTTCCAGTG ATGTATGGAT CCACCAATGG ATACAACAAG 
AATCTCTACA AGGCCAAGGA AGAGGACTAT GAAGGCTTGT ATTACCATGA CAACAACCTG 
ATATCTGGGT CCTTGGAAGC TCTTATTGAG CACTTGGTTC CTACAGTGGC GTACTATCCA 
GATAGGACAT ATATCTTCAC ATTCCTGCTC AGCTCGCGTC TCTTTCTTCA CCCATATGAG 
CTGATGTCCA AAGTGTGCCA TTTATGTGTG GACCAGCAGA GACTCAGTGA TCCTCAGGCA 
GACAAGGCTA GAGTCAGGAA AATTGCTCCC AAGATCCTGC AGTTGCTGAC TGAGTGGACG 
GAGACGTTCC CCTATGACTT TAGGGATGAG AGGATGATGA AGAGCCTGAA GGAACTAACA 
CATCGTCTGA GCACTGGAGA GGATCTGTAT CGTAAGGCGG TGCAACAGAT GACACAAACG 
CTGATCCGTA AGCTCACCAC ACTGAGTCAG TATGAAGAAG CGCTGGCCAA GATCAACTCC 
TCGGTGACGG ACAGACTAAC CGTGCTGAAG ACCAAACCGC AGTCCATCCA GAGAGACATT 
CTGACCATCT GCAACGACCC CTTCACCCTG GCACAACAAC TCACACACAT TGAACTGGAA 
AGGCTAAGCT ACATCGGACC GGAGGAGTTT GTTCAGGCGT TTGTTCAGAA AGACCCTCTT 
GATAATGACA AGAATTGCTT CAGTGATCAC AAGAAGGCAA GTAACCTGGA GGCTTACGTA 
GAGTGGTTCA ACAGGCTCAG CTACCTGGTG GCGACGGAGA TCTGCATGCC TGTCAAGAAG 
AAACATCGGG CTCGAGTCAT CGAATTCTTT ATCGACGTGG CACGAGAATG CTTCAACATT 
GGCAACTTCA ACTCGCTCAT GGCCATCATT ACCGGGATGA ACATGAGTCC TGTGTCGCGA 
CTCAAGAAGA CCTGGGCCAA AGTTAAAACC GCCAAATTTG ATATACTGGA GCACCAGATG 
GATCCCTCCA GCAATTTCTA CAACTACAGG ACGGCACTGC GCGGAGCCAC TCAGAGATCC 
ATCACTGCTC ACAGCAGCAG AGAGAAGATT GTTATCCCTT TCTTCAGCCT CCTAATTAAA 
GACATTTACT TCCTCAACGA AGGATGTGCC AACAGGCTCC CGAACGGACA TGTGAATTTT 
GAGAAATTCT GGGAACTAGC CAAACAGGTG ACAGAGTTCA TGACTTGGAA GAAGGTGGAG 
TGTCCTTTCG ACAGAGACCG CAAGATTCTG CAGTACCTCC TGACCGCTCC AGTCTTCAGT 
GAAGATGCTT TGTATCTGGC GTCATACGAG AGCGAAGGGC CCGAAAACAA CATGGAGAAA 
GACCGATGGA AATCTCTCAG GTCATCTTTG CTGAACAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165336.1
CDS	261..1805
Translation	MAKFLAELLGCTLPEKGASPMFECRSQPHIGLRHVTKQRDKMPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165279.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165279.3

ORF Insert Sequence:

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGGAC 
AAAATGCCTC AGACGCCACC CTTTCCAGTG ATGTATGGAT CCACCAATGG ATACAACAAG 
AATCTCTACA AGGCCAAGGA AGAGGACTAT GAAGGCTTGT ATTACCATGA CAACAACCTG 
ATATCTGGGT CCTTGGAAGC TCTTATTGAG CACTTGGTTC CTACAGTGGC GTACTATCCA 
GATAGGACAT ATATCTTCAC ATTCCTGCTC AGCTCGCGTC TCTTTCTTCA CCCATATGAG 
CTGATGTCCA AAGTGTGCCA TTTATGTGTG GACCAGCAGA GACTCAGTGA TCCTCAGGCA 
GACAAGGCTA GAGTCAGGAA AATTGCTCCC AAGATCCTGC AGTTGCTGAC TGAGTGGACG 
GAGACGTTCC CCTATGACTT TAGGGATGAG AGGATGATGA AGAGCCTGAA GGAACTAACA 
CATCGTCTGA GCACTGGAGA GGATCTGTAT CGTAAGGCGG TGCAACAGAT GACACAAACG 
CTGATCCGTA AGCTCACCAC ACTGAGTCAG TATGAAGAAG CGCTGGCCAA GATCAACTCC 
TCGGTGACGG ACAGACTAAC CGTGCTGAAG ACCAAACCGC AGTCCATCCA GAGAGACATT 
CTGACCATCT GCAACGACCC CTTCACCCTG GCACAACAAC TCACACACAT TGAACTGGAA 
AGGCTAAGCT ACATCGGACC GGAGGAGTTT GTTCAGGCGT TTGTTCAGAA AGACCCTCTT 
GATAATGACA AGAATTGCTT CAGTGATCAC AAGAAGGCAA GTAACCTGGA GGCTTACGTA 
GAGTGGTTCA ACAGGCTCAG CTACCTGGTG GCGACGGAGA TCTGCATGCC TGTCAAGAAG 
AAACATCGGG CTCGAGTCAT CGAATTCTTT ATCGACGTGG CACGAGAATG CTTCAACATT 
GGCAACTTCA ACTCGCTCAT GGCCATCATT ACCGGGATGA ACATGAGTCC TGTGTCGCGA 
CTCAAGAAGA CCTGGGCCAA AGTTAAAACC GCCAAATTTG ATATACTGGA GCACCAGATG 
GATCCCTCCA GCAATTTCTA CAACTACAGG ACGGCACTGC GCGGAGCCAC TCAGAGATCC 
ATCACTGCTC ACAGCAGCAG AGAGAAGATT GTTATCCCTT TCTTCAGCCT CCTAATTAAA 
GACATTTACT TCCTCAACGA AGGATGTGCC AACAGGCTCC CGAACGGACA TGTGAATTTT 
GAGAAATTCT GGGAACTAGC CAAACAGGTG ACAGAGTTCA TGACTTGGAA GAAGGTGGAG 
TGTCCTTTCG ACAGAGACCG CAAGATTCTG CAGTACCTCC TGACCGCTCC AGTCTTCAGT 
GAAGATGCTT TGTATCTGGC GTCATACGAG AGCGAAGGGC CCGAAAACAA CATGGAGAAA 
GACCGATGGA AATCTCTCAG GTCATCTTTG CTGAACAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165279.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165336.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10676
Clone ID Related Accession (Same CDS sequence)	XM_005165279.3 , XM_005165279.4
Accession Version	XM_005165279.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165279.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGGAC 
AAAATGCCTC AGACGCCACC CTTTCCAGTG ATGTATGGAT CCACCAATGG ATACAACAAG 
AATCTCTACA AGGCCAAGGA AGAGGACTAT GAAGGCTTGT ATTACCATGA CAACAACCTG 
ATATCTGGGT CCTTGGAAGC TCTTATTGAG CACTTGGTTC CTACAGTGGC GTACTATCCA 
GATAGGACAT ATATCTTCAC ATTCCTGCTC AGCTCGCGTC TCTTTCTTCA CCCATATGAG 
CTGATGTCCA AAGTGTGCCA TTTATGTGTG GACCAGCAGA GACTCAGTGA TCCTCAGGCA 
GACAAGGCTA GAGTCAGGAA AATTGCTCCC AAGATCCTGC AGTTGCTGAC TGAGTGGACG 
GAGACGTTCC CCTATGACTT TAGGGATGAG AGGATGATGA AGAGCCTGAA GGAACTAACA 
CATCGTCTGA GCACTGGAGA GGATCTGTAT CGTAAGGCGG TGCAACAGAT GACACAAACG 
CTGATCCGTA AGCTCACCAC ACTGAGTCAG TATGAAGAAG CGCTGGCCAA GATCAACTCC 
TCGGTGACGG ACAGACTAAC CGTGCTGAAG ACCAAACCGC AGTCCATCCA GAGAGACATT 
CTGACCATCT GCAACGACCC CTTCACCCTG GCACAACAAC TCACACACAT TGAACTGGAA 
AGGCTAAGCT ACATCGGACC GGAGGAGTTT GTTCAGGCGT TTGTTCAGAA AGACCCTCTT 
GATAATGACA AGAATTGCTT CAGTGATCAC AAGAAGGCAA GTAACCTGGA GGCTTACGTA 
GAGTGGTTCA ACAGGCTCAG CTACCTGGTG GCGACGGAGA TCTGCATGCC TGTCAAGAAG 
AAACATCGGG CTCGAGTCAT CGAATTCTTT ATCGACGTGG CACGAGAATG CTTCAACATT 
GGCAACTTCA ACTCGCTCAT GGCCATCATT ACCGGGATGA ACATGAGTCC TGTGTCGCGA 
CTCAAGAAGA CCTGGGCCAA AGTTAAAACC GCCAAATTTG ATATACTGGA GCACCAGATG 
GATCCCTCCA GCAATTTCTA CAACTACAGG ACGGCACTGC GCGGAGCCAC TCAGAGATCC 
ATCACTGCTC ACAGCAGCAG AGAGAAGATT GTTATCCCTT TCTTCAGCCT CCTAATTAAA 
GACATTTACT TCCTCAACGA AGGATGTGCC AACAGGCTCC CGAACGGACA TGTGAATTTT 
GAGAAATTCT GGGAACTAGC CAAACAGGTG ACAGAGTTCA TGACTTGGAA GAAGGTGGAG 
TGTCCTTTCG ACAGAGACCG CAAGATTCTG CAGTACCTCC TGACCGCTCC AGTCTTCAGT 
GAAGATGCTT TGTATCTGGC GTCATACGAG AGCGAAGGGC CCGAAAACAA CATGGAGAAA 
GACCGATGGA AATCTCTCAG GTCATCTTTG CTGAACAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165336.1
CDS	257..1801
Translation	MAKFLAELLGCTLPEKGASPMFECRSQPHIGLRHVTKQRDKMPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165279.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165279.4

ORF Insert Sequence:

ATGGCAAAGT TCCTCGCTGA GCTGCTGGGA TGTACACTGC CCGAGAAAGG AGCATCTCCC 
ATGTTCGAGT GCAGGAGTCA GCCACACATC GGACTGAGGC ACGTCACCAA ACAACGGGAC 
AAAATGCCTC AGACGCCACC CTTTCCAGTG ATGTATGGAT CCACCAATGG ATACAACAAG 
AATCTCTACA AGGCCAAGGA AGAGGACTAT GAAGGCTTGT ATTACCATGA CAACAACCTG 
ATATCTGGGT CCTTGGAAGC TCTTATTGAG CACTTGGTTC CTACAGTGGC GTACTATCCA 
GATAGGACAT ATATCTTCAC ATTCCTGCTC AGCTCGCGTC TCTTTCTTCA CCCATATGAG 
CTGATGTCCA AAGTGTGCCA TTTATGTGTG GACCAGCAGA GACTCAGTGA TCCTCAGGCA 
GACAAGGCTA GAGTCAGGAA AATTGCTCCC AAGATCCTGC AGTTGCTGAC TGAGTGGACG 
GAGACGTTCC CCTATGACTT TAGGGATGAG AGGATGATGA AGAGCCTGAA GGAACTAACA 
CATCGTCTGA GCACTGGAGA GGATCTGTAT CGTAAGGCGG TGCAACAGAT GACACAAACG 
CTGATCCGTA AGCTCACCAC ACTGAGTCAG TATGAAGAAG CGCTGGCCAA GATCAACTCC 
TCGGTGACGG ACAGACTAAC CGTGCTGAAG ACCAAACCGC AGTCCATCCA GAGAGACATT 
CTGACCATCT GCAACGACCC CTTCACCCTG GCACAACAAC TCACACACAT TGAACTGGAA 
AGGCTAAGCT ACATCGGACC GGAGGAGTTT GTTCAGGCGT TTGTTCAGAA AGACCCTCTT 
GATAATGACA AGAATTGCTT CAGTGATCAC AAGAAGGCAA GTAACCTGGA GGCTTACGTA 
GAGTGGTTCA ACAGGCTCAG CTACCTGGTG GCGACGGAGA TCTGCATGCC TGTCAAGAAG 
AAACATCGGG CTCGAGTCAT CGAATTCTTT ATCGACGTGG CACGAGAATG CTTCAACATT 
GGCAACTTCA ACTCGCTCAT GGCCATCATT ACCGGGATGA ACATGAGTCC TGTGTCGCGA 
CTCAAGAAGA CCTGGGCCAA AGTTAAAACC GCCAAATTTG ATATACTGGA GCACCAGATG 
GATCCCTCCA GCAATTTCTA CAACTACAGG ACGGCACTGC GCGGAGCCAC TCAGAGATCC 
ATCACTGCTC ACAGCAGCAG AGAGAAGATT GTTATCCCTT TCTTCAGCCT CCTAATTAAA 
GACATTTACT TCCTCAACGA AGGATGTGCC AACAGGCTCC CGAACGGACA TGTGAATTTT 
GAGAAATTCT GGGAACTAGC CAAACAGGTG ACAGAGTTCA TGACTTGGAA GAAGGTGGAG 
TGTCCTTTCG ACAGAGACCG CAAGATTCTG CAGTACCTCC TGACCGCTCC AGTCTTCAGT 
GAAGATGCTT TGTATCTGGC GTCATACGAG AGCGAAGGGC CCGAAAACAA CATGGAGAAA 
GACCGATGGA AATCTCTCAG GTCATCTTTG CTGAACAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165280.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165337.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10677
Clone ID Related Accession (Same CDS sequence)	XM_005165280.3 , XM_005165280.4
Accession Version	XM_005165280.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAAGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATATA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT ATGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTTAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCCAAAGT TAAAACCGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG AACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AAAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165337.1
CDS	275..1696
Translation	MPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165280.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165280.3

ORF Insert Sequence:

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAAGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATATA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT ATGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTTAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCCAAAGT TAAAACCGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG AACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AAAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasgef1ba ( XM_005165280.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165337.1 Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.

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CloneID	ODa10677
Clone ID Related Accession (Same CDS sequence)	XM_005165280.3 , XM_005165280.4
Accession Version	XM_005165280.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-GEF domain-containing family member 1B-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165280.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAAGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATATA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT ATGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTTAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCCAAAGT TAAAACCGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG AACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AAAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165337.1
CDS	278..1699
Translation	MPQTPPFPVMYGSTNGYNKNLYKAKEEDYEGLYYHDNNLISGSLEALIEHLVPTVAYYPDRTYIFTFLLSSRLFLHPYELMSKVCHLCVDQQRLSDPQADKARVRKIAPKILQLLTEWTETFPYDFRDERMMKSLKELTHRLSTGEDLYRKAVQQMTQTLIRKLTTLSQYEEALAKINSSVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNDKNCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIITGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVTEFMTWKKVECPFDRDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRSSLLNRT

Target ORF information:

RefSeq Version	XM_005165280.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RasGEF domain family, member 1Ba (rasgef1ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165280.4

ORF Insert Sequence:

ATGCCTCAGA CGCCACCCTT TCCAGTGATG TATGGATCCA CCAATGGATA CAACAAGAAT 
CTCTACAAGG CCAAGGAAGA GGACTATGAA GGCTTGTATT ACCATGACAA CAACCTGATA 
TCTGGGTCCT TGGAAGCTCT TATTGAGCAC TTGGTTCCTA CAGTGGCGTA CTATCCAGAT 
AGGACATATA TCTTCACATT CCTGCTCAGC TCGCGTCTCT TTCTTCACCC ATATGAGCTG 
ATGTCCAAAG TGTGCCATTT ATGTGTGGAC CAGCAGAGAC TCAGTGATCC TCAGGCAGAC 
AAGGCTAGAG TCAGGAAAAT TGCTCCCAAG ATCCTGCAGT TGCTGACTGA GTGGACGGAG 
ACGTTCCCCT ATGACTTTAG GGATGAGAGG ATGATGAAGA GCCTGAAGGA ACTAACACAT 
CGTCTGAGCA CTGGAGAGGA TCTGTATCGT AAGGCGGTGC AACAGATGAC ACAAACGCTG 
ATCCGTAAGC TCACCACACT GAGTCAGTAT GAAGAAGCGC TGGCCAAGAT CAACTCCTCG 
GTGACGGACA GACTAACCGT GCTGAAGACC AAACCGCAGT CCATCCAGAG AGACATTCTG 
ACCATCTGCA ACGACCCCTT CACCCTGGCA CAACAACTCA CACACATTGA ACTGGAAAGG 
CTAAGCTACA TCGGACCGGA GGAGTTTGTT CAGGCGTTTG TTCAGAAAGA CCCTCTTGAT 
AATGACAAGA ATTGCTTCAG TGATCACAAG AAGGCAAGTA ACCTGGAGGC TTACGTAGAG 
TGGTTCAACA GGCTCAGCTA CCTGGTGGCG ACGGAGATCT GCATGCCTGT CAAGAAGAAA 
CATCGGGCTC GAGTCATCGA ATTCTTTATC GACGTGGCAC GAGAATGCTT CAACATTGGC 
AACTTCAACT CGCTCATGGC CATCATTACC GGGATGAACA TGAGTCCTGT GTCGCGACTC 
AAGAAGACCT GGGCCAAAGT TAAAACCGCC AAATTTGATA TACTGGAGCA CCAGATGGAT 
CCCTCCAGCA ATTTCTACAA CTACAGGACG GCACTGCGCG GAGCCACTCA GAGATCCATC 
ACTGCTCACA GCAGCAGAGA GAAGATTGTT ATCCCTTTCT TCAGCCTCCT AATTAAAGAC 
ATTTACTTCC TCAACGAAGG ATGTGCCAAC AGGCTCCCGA ACGGACATGT GAATTTTGAG 
AAATTCTGGG AACTAGCCAA ACAGGTGACA GAGTTCATGA CTTGGAAGAA GGTGGAGTGT 
CCTTTCGACA GAGACCGCAA GATTCTGCAG TACCTCCTGA CCGCTCCAGT CTTCAGTGAA 
GATGCTTTGT ATCTGGCGTC ATACGAGAGC GAAGGGCCCG AAAACAACAT GGAGAAAGAC 
CGATGGAAAT CTCTCAGGTC ATCTTTGCTG AACAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.
eLife4(2015 Apr)
Dubrulle J,Jordan BM,Akhmetova L,Farrell JA,Kim SH,Solnica-Krezel L,Schier AF

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Quantitative genetic analysis of retinal degeneration in the blind cavefish Astyanax mexicanus.
PloS one8(2)e57281(2013)
O'Quin KE,Yoshizawa M,Doshi P,Jeffery WR

Evolution of follistatin in teleosts revealed through phylogenetic, genomic and expression analyses.
Development genes and evolution218(1)1-14(2008 Jan)
Macqueen DJ,Johnston IA

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rasgef1ba cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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