lrwd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrwd1
Entrez Gene ID	327124
Full Name	leucine-rich repeats and WD repeat domain containing 1
Synonyms	ORCA,si:dkey-261j4.9,wu:fb78b03,wu:ft31b04
General protein information	Preferred Names leucine-rich repeats and WD repeat domain containing 1 Names leucine-rich repeat and WD repeat-containing protein 1 ORC-associated protein origin recognition complex-associated protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_678221.7	XP_683313.4	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
XM_009301907.1	XP_009300182.1	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
NM_001305662.1	NP_001292591.1	leucine-rich repeat and WD repeat-containing protein 1
XM_678221.8	XP_683313.4	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
XM_017356041.1	XP_017211530.1	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
XM_021476013.1	XP_021331688.1	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
XM_017356041.2	XP_017211530.1	leucine-rich repeat and WD repeat-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LRWD1	XP_519556.3
	LRWD1	XP_001108941.1
Bos taurus (cattle)	LRWD1	NP_001178398.1
Gallus gallus (chicken)	LRWD1	XP_415759.2
Homo sapiens (human)	LRWD1	NP_690852.1
Mus musculus (house mouse)	Lrwd1	NP_082167.2
Canis lupus familiaris (dog)	LRWD1	XP_536851.3
Danio rerio (zebrafish)	lrwd1	XP_683313.4
Xenopus tropicalis (tropical clawed frog)	lrwd1	NP_001120130.1

Gene Ontology
Bibliography

Related articles in PubMed

Uniform gene expression in embryos is achieved by temporal averaging of transcription noise.
Stapel LC, Zechner C, Vastenhouw NL
Genes & development31(16)1635-1640(2017 Aug)

The guanine nucleotide exchange factor Net1 facilitates the specification of dorsal cell fates in zebrafish embryos by promoting maternal ?-catenin activation.
Wei S, Dai M, Liu Z, Ma Y, Shang H, Cao Y, Wang Q
Cell research27(2)202-225(2017 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Cadm4 restricts the production of cardiac outflow tract progenitor cells.
Zeng XX, Yelon D
Cell reports7(4)951-60(2014 May)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lrwd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrwd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39896	NM_001305662.1 Latest version!	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa11105	XM_678221.8 Latest version!	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39896	XM_009301907.1 Latest version!	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39896	XM_017356041.2 Latest version!	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39896	XM_021476013.1 Latest version!	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa11105	XM_678221.7	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39896	XM_017356041.1	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrwd1 ( NM_001305662.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001292591.1 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), mRNA.

Price & Availability↑

CloneID	ODa39896
Clone ID Related Accession (Same CDS sequence)	XM_009301907.1 , NM_001305662.1 , XM_017356041.1 , XM_021476013.1 , XM_017356041.2
Accession Version	NM_001305662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935280.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC125814.1, BC095309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292591.1
CDS	3..2294
Translation	MEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	NM_001305662.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305662.1

ORF Insert Sequence:

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_678221.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683313.4 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11105
Clone ID Related Accession (Same CDS sequence)	XM_678221.7 , XM_678221.8
Accession Version	XM_678221.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_678221.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATATTA CGGCCATGGA GAAGATTACC GAGAAGATAC TGCTGGAGAA AAGCTCTACA 
AAGACGAACA AACTGGACCA GATCAAGACC CTCAATCTGT CCCGCATGAG TCTGAAGAGC 
GAGGATCTGC CTGTGCCTCT GCTGTCTAAA CTGTGTCGTC TGGAGAAGTT GGATTTGTCT 
GGGAATATGT TGCAGAAGAT CCCCAAAGGC CTCCGTCTGC CCTGCCTGAA GATCCTGAAC 
TGCTCAAACA ATGATATGGA GGATGTGTTG TCGCTGGAAG CCCTGACAAA CTTAGAGGAA 
CTTAGACTAG AGGACAACCT TTACCTCACA GTCAATGATG AACACAAAGT GATCTTCCTG 
TTACCTAATC TGAGGATGTT CAATGGGAAG GATATCAGTT CAACAGCTCA TCATATTCGC 
CATGGGAGCA CTGAGATCCT CAGGAAGCGG GTGATTGGTG TGTGGGAACG AGACTTTAGT 
CTTCCTGATC CCATTTCAGC AAAAAGCCTT GCTGCTGTTG AGAAGAGCTT TGTTAACGCT 
GCCTGCACAC AAGTCAAGTA TGGCCCCAAT TCCTTGAGTG ATTACACAAA GTGGAGGGTT 
GAAAAGATTG CAAAGGAGTA TTTGAAGTCA CTGACCAGTA GTGAGGAGGA AGAACGGGTT 
GCAGACACAA CTCCAACAAA GGAGAATAAG ACAAAGGCCT GTGATGTTGG TGGCAACAGT 
ATCACAAGTC CTCAAAAACG AACCAGAAAC AACACTGATG TGGTTGCAGA GGCCAGTCCA 
CGAAAGTCAA GTCGCCTGGT GAGTGCAGCT CCGGTTGAGG CTAGTCCACG AAAATCAGCT 
CGCGTGTTGA ACACTCCACA GAAAACTCAG CCTGTAGTGT CCAGCCCCAG AAAGCACGCC 
AGACTGACCA GTGCAGAGAC TCCGGAGAGC AGTCCTAGAA AGTCAAGTCG CTTGGAAAAT 
GTTACTCAGA AAGCTGCTAG CCAAACAGAA AGCCCAAGAA AGCCAGGAAT GTCTACACCC 
ACATCAAAAC AGGCTAAATG TGAGAGCCCA AGAAAACAGA GCAAACAGAG CACCGCCAAA 
ATGGAGAAAA GCACACCTCG GAAAACTACC AAAGCCAAAC TTCAAGTTCC TCAGGAGCCG 
GTGAGTCTGA CACCTCTTCA TGTCCTGCAG TGTCACAGTC GACAGAACGA TCCAGACGAC 
TTCAGCACTC AGCTTTGGGC CTGTGCTTTT GAGCCCCAGC AGGACGACAG TATCGATATC 
AGTGGTGGCT CTCAGACAAT TGCCACATGT GGAGGAGAAA CTTTGTGTGT GATCAACTGT 
GAGTCAGGAC TGGTGCTGAA GAAATACAAG GTTCCTGGTG AGGATTTTTT CTCTTTAGCC 
TGGTCCACTG TGCTGATGTC CAGAACAGGT GGCTCTGCCC GTCCCTGCAA TATCCTGGCT 
GCTGGAGGAA AGAGAGGTTG TGTGAAGCTC ATCCATCCCC GAGTCAACCT GGCATTTGGA 
GAGTTTCGCG TCAGTCGACG TGCCATTTCC ATAATGCGCT TCAATCCACG CAAACCCACC 
TTTCTCTTCA CTGGGACGTA TGATAAAAAG ATCTTTTTGT GGGACATTGG TGGACTGGAT 
CAGGATTACA ACTTTAAAAT CAGTAAGCTG CTAACACTGG AGACGAGCTC CACTCCTCTT 
CATCTCGCTC TGCTCCCCTC GTCCCCAGAC ACACATCTGC TTTCAGGCTG TGATGAAGGC 
CTGTATTGCT TTGACGTCCA GCTCAGTAAA AACACACTGA AGAGAAATGA AGAGATTGAG 
ATCGTATTTC CAATTTACAA GAAAAATGAC AAGAAAAACA ACTACCGGAC TATTGATGGA 
TTGAGCTTCC TTTCTGATGA TGTAGTGGCA TCCAAGAGCC ACATGCAGGG CTCAATCTAC 
CTGTGGAGCT GGAGTGCCAC ACGGGCATCA TGGAACAGCA GAAAGAAAGA GGTGCCGGCT 
GTTATTCTGG CAGAGCTGCA ATGGTCCAGT ACTGACATCC CATACCTGTC TCTAGGCACC 
TGTCCAGGTT ATGGTTATGT AGTATGTGGA GATGAGCAAG GCAGACTATG GATGTATCAT 
ATTACAGACA CCATGATGGA AAACTTCAAG AGTGGAAAAA CAATCTCAGC CACTGAGGTG 
CTGCAGTGGC CGTCACCTAT CAGAGCTGGA AAGGGTGCTT TAGAGGGGCC GTCTATTAAC 
AGCACCGCCA TGGATCCTGG TCTCCATTAT TTGGTGGCCC TTACAGACAA GAACATGGTT 
GTGGTTTGGA AAAGAGAGAG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683313.4
CDS	99..2405
Translation	MHITAMEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_678221.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678221.7

ORF Insert Sequence:

ATGCATATTA CGGCCATGGA GAAGATTACC GAGAAGATAC TGCTGGAGAA AAGCTCTACA 
AAGACGAACA AACTGGACCA GATCAAGACC CTCAATCTGT CCCGCATGAG TCTGAAGAGC 
GAGGATCTGC CTGTGCCTCT GCTGTCTAAA CTGTGTCGTC TGGAGAAGTT GGATTTGTCT 
GGGAATATGT TGCAGAAGAT CCCCAAAGGC CTCCGTCTGC CCTGCCTGAA GATCCTGAAC 
TGCTCAAACA ATGATATGGA GGATGTGTTG TCGCTGGAAG CCCTGACAAA CTTAGAGGAA 
CTTAGACTAG AGGACAACCT TTACCTCACA GTCAATGATG AACACAAAGT GATCTTCCTG 
TTACCTAATC TGAGGATGTT CAATGGGAAG GATATCAGTT CAACAGCTCA TCATATTCGC 
CATGGGAGCA CTGAGATCCT CAGGAAGCGG GTGATTGGTG TGTGGGAACG AGACTTTAGT 
CTTCCTGATC CCATTTCAGC AAAAAGCCTT GCTGCTGTTG AGAAGAGCTT TGTTAACGCT 
GCCTGCACAC AAGTCAAGTA TGGCCCCAAT TCCTTGAGTG ATTACACAAA GTGGAGGGTT 
GAAAAGATTG CAAAGGAGTA TTTGAAGTCA CTGACCAGTA GTGAGGAGGA AGAACGGGTT 
GCAGACACAA CTCCAACAAA GGAGAATAAG ACAAAGGCCT GTGATGTTGG TGGCAACAGT 
ATCACAAGTC CTCAAAAACG AACCAGAAAC AACACTGATG TGGTTGCAGA GGCCAGTCCA 
CGAAAGTCAA GTCGCCTGGT GAGTGCAGCT CCGGTTGAGG CTAGTCCACG AAAATCAGCT 
CGCGTGTTGA ACACTCCACA GAAAACTCAG CCTGTAGTGT CCAGCCCCAG AAAGCACGCC 
AGACTGACCA GTGCAGAGAC TCCGGAGAGC AGTCCTAGAA AGTCAAGTCG CTTGGAAAAT 
GTTACTCAGA AAGCTGCTAG CCAAACAGAA AGCCCAAGAA AGCCAGGAAT GTCTACACCC 
ACATCAAAAC AGGCTAAATG TGAGAGCCCA AGAAAACAGA GCAAACAGAG CACCGCCAAA 
ATGGAGAAAA GCACACCTCG GAAAACTACC AAAGCCAAAC TTCAAGTTCC TCAGGAGCCG 
GTGAGTCTGA CACCTCTTCA TGTCCTGCAG TGTCACAGTC GACAGAACGA TCCAGACGAC 
TTCAGCACTC AGCTTTGGGC CTGTGCTTTT GAGCCCCAGC AGGACGACAG TATCGATATC 
AGTGGTGGCT CTCAGACAAT TGCCACATGT GGAGGAGAAA CTTTGTGTGT GATCAACTGT 
GAGTCAGGAC TGGTGCTGAA GAAATACAAG GTTCCTGGTG AGGATTTTTT CTCTTTAGCC 
TGGTCCACTG TGCTGATGTC CAGAACAGGT GGCTCTGCCC GTCCCTGCAA TATCCTGGCT 
GCTGGAGGAA AGAGAGGTTG TGTGAAGCTC ATCCATCCCC GAGTCAACCT GGCATTTGGA 
GAGTTTCGCG TCAGTCGACG TGCCATTTCC ATAATGCGCT TCAATCCACG CAAACCCACC 
TTTCTCTTCA CTGGGACGTA TGATAAAAAG ATCTTTTTGT GGGACATTGG TGGACTGGAT 
CAGGATTACA ACTTTAAAAT CAGTAAGCTG CTAACACTGG AGACGAGCTC CACTCCTCTT 
CATCTCGCTC TGCTCCCCTC GTCCCCAGAC ACACATCTGC TTTCAGGCTG TGATGAAGGC 
CTGTATTGCT TTGACGTCCA GCTCAGTAAA AACACACTGA AGAGAAATGA AGAGATTGAG 
ATCGTATTTC CAATTTACAA GAAAAATGAC AAGAAAAACA ACTACCGGAC TATTGATGGA 
TTGAGCTTCC TTTCTGATGA TGTAGTGGCA TCCAAGAGCC ACATGCAGGG CTCAATCTAC 
CTGTGGAGCT GGAGTGCCAC ACGGGCATCA TGGAACAGCA GAAAGAAAGA GGTGCCGGCT 
GTTATTCTGG CAGAGCTGCA ATGGTCCAGT ACTGACATCC CATACCTGTC TCTAGGCACC 
TGTCCAGGTT ATGGTTATGT AGTATGTGGA GATGAGCAAG GCAGACTATG GATGTATCAT 
ATTACAGACA CCATGATGGA AAACTTCAAG AGTGGAAAAA CAATCTCAGC CACTGAGGTG 
CTGCAGTGGC CGTCACCTAT CAGAGCTGGA AAGGGTGCTT TAGAGGGGCC GTCTATTAAC 
AGCACCGCCA TGGATCCTGG TCTCCATTAT TTGGTGGCCC TTACAGACAA GAACATGGTT 
GTGGTTTGGA AAAGAGAGAG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_678221.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683313.4 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11105
Clone ID Related Accession (Same CDS sequence)	XM_678221.7 , XM_678221.8
Accession Version	XM_678221.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_678221.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATATTA CGGCCATGGA GAAGATTACC GAGAAGATAC TGCTGGAGAA AAGCTCTACA 
AAGACGAACA AACTGGACCA GATCAAGACC CTCAATCTGT CCCGCATGAG TCTGAAGAGC 
GAGGATCTGC CTGTGCCTCT GCTGTCTAAA CTGTGTCGTC TGGAGAAGTT GGATTTGTCT 
GGGAATATGT TGCAGAAGAT CCCCAAAGGC CTCCGTCTGC CCTGCCTGAA GATCCTGAAC 
TGCTCAAACA ATGATATGGA GGATGTGTTG TCGCTGGAAG CCCTGACAAA CTTAGAGGAA 
CTTAGACTAG AGGACAACCT TTACCTCACA GTCAATGATG AACACAAAGT GATCTTCCTG 
TTACCTAATC TGAGGATGTT CAATGGGAAG GATATCAGTT CAACAGCTCA TCATATTCGC 
CATGGGAGCA CTGAGATCCT CAGGAAGCGG GTGATTGGTG TGTGGGAACG AGACTTTAGT 
CTTCCTGATC CCATTTCAGC AAAAAGCCTT GCTGCTGTTG AGAAGAGCTT TGTTAACGCT 
GCCTGCACAC AAGTCAAGTA TGGCCCCAAT TCCTTGAGTG ATTACACAAA GTGGAGGGTT 
GAAAAGATTG CAAAGGAGTA TTTGAAGTCA CTGACCAGTA GTGAGGAGGA AGAACGGGTT 
GCAGACACAA CTCCAACAAA GGAGAATAAG ACAAAGGCCT GTGATGTTGG TGGCAACAGT 
ATCACAAGTC CTCAAAAACG AACCAGAAAC AACACTGATG TGGTTGCAGA GGCCAGTCCA 
CGAAAGTCAA GTCGCCTGGT GAGTGCAGCT CCGGTTGAGG CTAGTCCACG AAAATCAGCT 
CGCGTGTTGA ACACTCCACA GAAAACTCAG CCTGTAGTGT CCAGCCCCAG AAAGCACGCC 
AGACTGACCA GTGCAGAGAC TCCGGAGAGC AGTCCTAGAA AGTCAAGTCG CTTGGAAAAT 
GTTACTCAGA AAGCTGCTAG CCAAACAGAA AGCCCAAGAA AGCCAGGAAT GTCTACACCC 
ACATCAAAAC AGGCTAAATG TGAGAGCCCA AGAAAACAGA GCAAACAGAG CACCGCCAAA 
ATGGAGAAAA GCACACCTCG GAAAACTACC AAAGCCAAAC TTCAAGTTCC TCAGGAGCCG 
GTGAGTCTGA CACCTCTTCA TGTCCTGCAG TGTCACAGTC GACAGAACGA TCCAGACGAC 
TTCAGCACTC AGCTTTGGGC CTGTGCTTTT GAGCCCCAGC AGGACGACAG TATCGATATC 
AGTGGTGGCT CTCAGACAAT TGCCACATGT GGAGGAGAAA CTTTGTGTGT GATCAACTGT 
GAGTCAGGAC TGGTGCTGAA GAAATACAAG GTTCCTGGTG AGGATTTTTT CTCTTTAGCC 
TGGTCCACTG TGCTGATGTC CAGAACAGGT GGCTCTGCCC GTCCCTGCAA TATCCTGGCT 
GCTGGAGGAA AGAGAGGTTG TGTGAAGCTC ATCCATCCCC GAGTCAACCT GGCATTTGGA 
GAGTTTCGCG TCAGTCGACG TGCCATTTCC ATAATGCGCT TCAATCCACG CAAACCCACC 
TTTCTCTTCA CTGGGACGTA TGATAAAAAG ATCTTTTTGT GGGACATTGG TGGACTGGAT 
CAGGATTACA ACTTTAAAAT CAGTAAGCTG CTAACACTGG AGACGAGCTC CACTCCTCTT 
CATCTCGCTC TGCTCCCCTC GTCCCCAGAC ACACATCTGC TTTCAGGCTG TGATGAAGGC 
CTGTATTGCT TTGACGTCCA GCTCAGTAAA AACACACTGA AGAGAAATGA AGAGATTGAG 
ATCGTATTTC CAATTTACAA GAAAAATGAC AAGAAAAACA ACTACCGGAC TATTGATGGA 
TTGAGCTTCC TTTCTGATGA TGTAGTGGCA TCCAAGAGCC ACATGCAGGG CTCAATCTAC 
CTGTGGAGCT GGAGTGCCAC ACGGGCATCA TGGAACAGCA GAAAGAAAGA GGTGCCGGCT 
GTTATTCTGG CAGAGCTGCA ATGGTCCAGT ACTGACATCC CATACCTGTC TCTAGGCACC 
TGTCCAGGTT ATGGTTATGT AGTATGTGGA GATGAGCAAG GCAGACTATG GATGTATCAT 
ATTACAGACA CCATGATGGA AAACTTCAAG AGTGGAAAAA CAATCTCAGC CACTGAGGTG 
CTGCAGTGGC CGTCACCTAT CAGAGCTGGA AAGGGTGCTT TAGAGGGGCC GTCTATTAAC 
AGCACCGCCA TGGATCCTGG TCTCCATTAT TTGGTGGCCC TTACAGACAA GAACATGGTT 
GTGGTTTGGA AAAGAGAGAG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683313.4
CDS	125..2431
Translation	MHITAMEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_678221.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678221.8

ORF Insert Sequence:

ATGCATATTA CGGCCATGGA GAAGATTACC GAGAAGATAC TGCTGGAGAA AAGCTCTACA 
AAGACGAACA AACTGGACCA GATCAAGACC CTCAATCTGT CCCGCATGAG TCTGAAGAGC 
GAGGATCTGC CTGTGCCTCT GCTGTCTAAA CTGTGTCGTC TGGAGAAGTT GGATTTGTCT 
GGGAATATGT TGCAGAAGAT CCCCAAAGGC CTCCGTCTGC CCTGCCTGAA GATCCTGAAC 
TGCTCAAACA ATGATATGGA GGATGTGTTG TCGCTGGAAG CCCTGACAAA CTTAGAGGAA 
CTTAGACTAG AGGACAACCT TTACCTCACA GTCAATGATG AACACAAAGT GATCTTCCTG 
TTACCTAATC TGAGGATGTT CAATGGGAAG GATATCAGTT CAACAGCTCA TCATATTCGC 
CATGGGAGCA CTGAGATCCT CAGGAAGCGG GTGATTGGTG TGTGGGAACG AGACTTTAGT 
CTTCCTGATC CCATTTCAGC AAAAAGCCTT GCTGCTGTTG AGAAGAGCTT TGTTAACGCT 
GCCTGCACAC AAGTCAAGTA TGGCCCCAAT TCCTTGAGTG ATTACACAAA GTGGAGGGTT 
GAAAAGATTG CAAAGGAGTA TTTGAAGTCA CTGACCAGTA GTGAGGAGGA AGAACGGGTT 
GCAGACACAA CTCCAACAAA GGAGAATAAG ACAAAGGCCT GTGATGTTGG TGGCAACAGT 
ATCACAAGTC CTCAAAAACG AACCAGAAAC AACACTGATG TGGTTGCAGA GGCCAGTCCA 
CGAAAGTCAA GTCGCCTGGT GAGTGCAGCT CCGGTTGAGG CTAGTCCACG AAAATCAGCT 
CGCGTGTTGA ACACTCCACA GAAAACTCAG CCTGTAGTGT CCAGCCCCAG AAAGCACGCC 
AGACTGACCA GTGCAGAGAC TCCGGAGAGC AGTCCTAGAA AGTCAAGTCG CTTGGAAAAT 
GTTACTCAGA AAGCTGCTAG CCAAACAGAA AGCCCAAGAA AGCCAGGAAT GTCTACACCC 
ACATCAAAAC AGGCTAAATG TGAGAGCCCA AGAAAACAGA GCAAACAGAG CACCGCCAAA 
ATGGAGAAAA GCACACCTCG GAAAACTACC AAAGCCAAAC TTCAAGTTCC TCAGGAGCCG 
GTGAGTCTGA CACCTCTTCA TGTCCTGCAG TGTCACAGTC GACAGAACGA TCCAGACGAC 
TTCAGCACTC AGCTTTGGGC CTGTGCTTTT GAGCCCCAGC AGGACGACAG TATCGATATC 
AGTGGTGGCT CTCAGACAAT TGCCACATGT GGAGGAGAAA CTTTGTGTGT GATCAACTGT 
GAGTCAGGAC TGGTGCTGAA GAAATACAAG GTTCCTGGTG AGGATTTTTT CTCTTTAGCC 
TGGTCCACTG TGCTGATGTC CAGAACAGGT GGCTCTGCCC GTCCCTGCAA TATCCTGGCT 
GCTGGAGGAA AGAGAGGTTG TGTGAAGCTC ATCCATCCCC GAGTCAACCT GGCATTTGGA 
GAGTTTCGCG TCAGTCGACG TGCCATTTCC ATAATGCGCT TCAATCCACG CAAACCCACC 
TTTCTCTTCA CTGGGACGTA TGATAAAAAG ATCTTTTTGT GGGACATTGG TGGACTGGAT 
CAGGATTACA ACTTTAAAAT CAGTAAGCTG CTAACACTGG AGACGAGCTC CACTCCTCTT 
CATCTCGCTC TGCTCCCCTC GTCCCCAGAC ACACATCTGC TTTCAGGCTG TGATGAAGGC 
CTGTATTGCT TTGACGTCCA GCTCAGTAAA AACACACTGA AGAGAAATGA AGAGATTGAG 
ATCGTATTTC CAATTTACAA GAAAAATGAC AAGAAAAACA ACTACCGGAC TATTGATGGA 
TTGAGCTTCC TTTCTGATGA TGTAGTGGCA TCCAAGAGCC ACATGCAGGG CTCAATCTAC 
CTGTGGAGCT GGAGTGCCAC ACGGGCATCA TGGAACAGCA GAAAGAAAGA GGTGCCGGCT 
GTTATTCTGG CAGAGCTGCA ATGGTCCAGT ACTGACATCC CATACCTGTC TCTAGGCACC 
TGTCCAGGTT ATGGTTATGT AGTATGTGGA GATGAGCAAG GCAGACTATG GATGTATCAT 
ATTACAGACA CCATGATGGA AAACTTCAAG AGTGGAAAAA CAATCTCAGC CACTGAGGTG 
CTGCAGTGGC CGTCACCTAT CAGAGCTGGA AAGGGTGCTT TAGAGGGGCC GTCTATTAAC 
AGCACCGCCA TGGATCCTGG TCTCCATTAT TTGGTGGCCC TTACAGACAA GAACATGGTT 
GTGGTTTGGA AAAGAGAGAG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_009301907.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300182.1 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39896
Clone ID Related Accession (Same CDS sequence)	XM_009301907.1 , NM_001305662.1 , XM_017356041.1 , XM_021476013.1 , XM_017356041.2
Accession Version	XM_009301907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
Comment

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_009300182.1

Translation

MEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_009301907.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301907.1

ORF Insert Sequence:

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_017356041.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211530.1 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39896
Clone ID Related Accession (Same CDS sequence)	XM_009301907.1 , NM_001305662.1 , XM_017356041.1 , XM_021476013.1 , XM_017356041.2
Accession Version	XM_017356041.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211530.1
CDS	125..2416
Translation	MEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_017356041.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356041.1

ORF Insert Sequence:

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_021476013.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331688.1 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39896
Clone ID Related Accession (Same CDS sequence)	XM_009301907.1 , NM_001305662.1 , XM_017356041.1 , XM_021476013.1 , XM_017356041.2
Accession Version	XM_021476013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331688.1
CDS	101..2392
Translation	MEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_021476013.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476013.1

ORF Insert Sequence:

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrwd1 ( XM_017356041.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211530.1 Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39896
Clone ID Related Accession (Same CDS sequence)	XM_009301907.1 , NM_001305662.1 , XM_017356041.1 , XM_021476013.1 , XM_017356041.2
Accession Version	XM_017356041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211530.1
CDS	181..2472
Translation	MEKITEKILLEKSSTKTNKLDQIKTLNLSRMSLKSEDLPVPLLSKLCRLEKLDLSGNMLQKIPKGLRLPCLKILNCSNNDMEDVLSLEALTNLEELRLEDNLYLTVNDEHKVIFLLPNLRMFNGKDISSTAHHIRHGSTEILRKRVIGVWERDFSLPDPISAKSLAAVEKSFVNAACTQVKYGPNSLSDYTKWRVEKIAKEYLKSLTSSEEEERVADTTPTKENKTKACDVGGNSITSPQKRTRNNTDVVAEASPRKSSRLVSAAPVEASPRKSARVLNTPQKTQPVVSSPRKHARLTSAETPESSPRKSSRLENVTQKAASQTESPRKPGMSTPTSKQAKCESPRKQSKQSTAKMEKSTPRKTTKAKLQVPQEPVSLTPLHVLQCHSRQNDPDDFSTQLWACAFEPQQDDSIDISGGSQTIATCGGETLCVINCESGLVLKKYKVPGEDFFSLAWSTVLMSRTGGSARPCNILAAGGKRGCVKLIHPRVNLAFGEFRVSRRAISIMRFNPRKPTFLFTGTYDKKIFLWDIGGLDQDYNFKISKLLTLETSSTPLHLALLPSSPDTHLLSGCDEGLYCFDVQLSKNTLKRNEEIEIVFPIYKKNDKKNNYRTIDGLSFLSDDVVASKSHMQGSIYLWSWSATRASWNSRKKEVPAVILAELQWSSTDIPYLSLGTCPGYGYVVCGDEQGRLWMYHITDTMMENFKSGKTISATEVLQWPSPIRAGKGALEGPSINSTAMDPGLHYLVALTDKNMVVVWKRESH

Target ORF information:

RefSeq Version	XM_017356041.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and WD repeat domain containing 1 (lrwd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356041.2

ORF Insert Sequence:

ATGGAGAAGA TTACCGAGAA GATACTGCTG GAGAAAAGCT CTACAAAGAC GAACAAACTG 
GACCAGATCA AGACCCTCAA TCTGTCCCGC ATGAGTCTGA AGAGCGAGGA TCTGCCTGTG 
CCTCTGCTGT CTAAACTGTG TCGTCTGGAG AAGTTGGATT TGTCTGGGAA TATGTTGCAG 
AAGATCCCCA AAGGCCTCCG TCTGCCCTGC CTGAAGATCC TGAACTGCTC AAACAATGAT 
ATGGAGGATG TGTTGTCGCT GGAAGCCCTG ACAAACTTAG AGGAACTTAG ACTAGAGGAC 
AACCTTTACC TCACAGTCAA TGATGAACAC AAAGTGATCT TCCTGTTACC TAATCTGAGG 
ATGTTCAATG GGAAGGATAT CAGTTCAACA GCTCATCATA TTCGCCATGG GAGCACTGAG 
ATCCTCAGGA AGCGGGTGAT TGGTGTGTGG GAACGAGACT TTAGTCTTCC TGATCCCATT 
TCAGCAAAAA GCCTTGCTGC TGTTGAGAAG AGCTTTGTTA ACGCTGCCTG CACACAAGTC 
AAGTATGGCC CCAATTCCTT GAGTGATTAC ACAAAGTGGA GGGTTGAAAA GATTGCAAAG 
GAGTATTTGA AGTCACTGAC CAGTAGTGAG GAGGAAGAAC GGGTTGCAGA CACAACTCCA 
ACAAAGGAGA ATAAGACAAA GGCCTGTGAT GTTGGTGGCA ACAGTATCAC AAGTCCTCAA 
AAACGAACCA GAAACAACAC TGATGTGGTT GCAGAGGCCA GTCCACGAAA GTCAAGTCGC 
CTGGTGAGTG CAGCTCCGGT TGAGGCTAGT CCACGAAAAT CAGCTCGCGT GTTGAACACT 
CCACAGAAAA CTCAGCCTGT AGTGTCCAGC CCCAGAAAGC ACGCCAGACT GACCAGTGCA 
GAGACTCCGG AGAGCAGTCC TAGAAAGTCA AGTCGCTTGG AAAATGTTAC TCAGAAAGCT 
GCTAGCCAAA CAGAAAGCCC AAGAAAGCCA GGAATGTCTA CACCCACATC AAAACAGGCT 
AAATGTGAGA GCCCAAGAAA ACAGAGCAAA CAGAGCACCG CCAAAATGGA GAAAAGCACA 
CCTCGGAAAA CTACCAAAGC CAAACTTCAA GTTCCTCAGG AGCCGGTGAG TCTGACACCT 
CTTCATGTCC TGCAGTGTCA CAGTCGACAG AACGATCCAG ACGACTTCAG CACTCAGCTT 
TGGGCCTGTG CTTTTGAGCC CCAGCAGGAC GACAGTATCG ATATCAGTGG TGGCTCTCAG 
ACAATTGCCA CATGTGGAGG AGAAACTTTG TGTGTGATCA ACTGTGAGTC AGGACTGGTG 
CTGAAGAAAT ACAAGGTTCC TGGTGAGGAT TTTTTCTCTT TAGCCTGGTC CACTGTGCTG 
ATGTCCAGAA CAGGTGGCTC TGCCCGTCCC TGCAATATCC TGGCTGCTGG AGGAAAGAGA 
GGTTGTGTGA AGCTCATCCA TCCCCGAGTC AACCTGGCAT TTGGAGAGTT TCGCGTCAGT 
CGACGTGCCA TTTCCATAAT GCGCTTCAAT CCACGCAAAC CCACCTTTCT CTTCACTGGG 
ACGTATGATA AAAAGATCTT TTTGTGGGAC ATTGGTGGAC TGGATCAGGA TTACAACTTT 
AAAATCAGTA AGCTGCTAAC ACTGGAGACG AGCTCCACTC CTCTTCATCT CGCTCTGCTC 
CCCTCGTCCC CAGACACACA TCTGCTTTCA GGCTGTGATG AAGGCCTGTA TTGCTTTGAC 
GTCCAGCTCA GTAAAAACAC ACTGAAGAGA AATGAAGAGA TTGAGATCGT ATTTCCAATT 
TACAAGAAAA ATGACAAGAA AAACAACTAC CGGACTATTG ATGGATTGAG CTTCCTTTCT 
GATGATGTAG TGGCATCCAA GAGCCACATG CAGGGCTCAA TCTACCTGTG GAGCTGGAGT 
GCCACACGGG CATCATGGAA CAGCAGAAAG AAAGAGGTGC CGGCTGTTAT TCTGGCAGAG 
CTGCAATGGT CCAGTACTGA CATCCCATAC CTGTCTCTAG GCACCTGTCC AGGTTATGGT 
TATGTAGTAT GTGGAGATGA GCAAGGCAGA CTATGGATGT ATCATATTAC AGACACCATG 
ATGGAAAACT TCAAGAGTGG AAAAACAATC TCAGCCACTG AGGTGCTGCA GTGGCCGTCA 
CCTATCAGAG CTGGAAAGGG TGCTTTAGAG GGGCCGTCTA TTAACAGCAC CGCCATGGAT 
CCTGGTCTCC ATTATTTGGT GGCCCTTACA GACAAGAACA TGGTTGTGGT TTGGAAAAGA 
GAGAGTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Uniform gene expression in embryos is achieved by temporal averaging of transcription noise.
Genes & development31(16)1635-1640(2017 Aug)
Stapel LC,Zechner C,Vastenhouw NL

The guanine nucleotide exchange factor Net1 facilitates the specification of dorsal cell fates in zebrafish embryos by promoting maternal ?-catenin activation.
Cell research27(2)202-225(2017 Feb)
Wei S,Dai M,Liu Z,Ma Y,Shang H,Cao Y,Wang Q

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Cadm4 restricts the production of cardiac outflow tract progenitor cells.
Cell reports7(4)951-60(2014 May)
Zeng XX,Yelon D

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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lrwd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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