sppl2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sppl2
Entrez Gene ID	325540
Full Name	signal peptide peptidase-like 2
Synonyms	SPPL2B,wu:fc16e01,wu:fc85d12,zgc:136525
General protein information	Preferred Names signal peptide peptidase-like 2 Names signal peptide peptidase-like 2B signal peptide peptidase-like protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045244.2	NP_001038709.2	signal peptide peptidase-like 2B isoform 2 precursor
XM_021478906.1	XP_021334581.1	signal peptide peptidase-like 2B isoform X1
XM_021478911.1	XP_021334586.1	signal peptide peptidase-like 2B isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sppl2	NP_001015067.1
Homo sapiens (human)	SPPL2B	NP_694533.1
Canis lupus familiaris (dog)	SPPL2B	XP_542189.4
Mus musculus (house mouse)	Sppl2b	NP_780404.2
Rattus norvegicus (Norway rat)	Sppl2b	NP_001014222.1
Arabidopsis thaliana (thale cress)	SPPL5	NP_172073.2
Xenopus tropicalis (tropical clawed frog)	sppl2b	NP_001072874.1
Pan troglodytes (chimpanzee)	SPPL2B	XP_003316040.2
Bos taurus (cattle)	SPPL2B	XP_002689000.2
Arabidopsis thaliana (thale cress)	SPPL3	NP_850383.2
Oryza sativa (rice)	Os01g0914700	NP_001045182.1
Gallus gallus (chicken)	SPPL2B	NP_001026104.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Cellular functions of gamma-secretase-related proteins.
Haffner C, Haass C
Neuro-degenerative diseases3(4-5)284-9(2006)

Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.
Krawitz P, Haffner C, Fluhrer R, Steiner H, Schmid B, Haass C
The Journal of biological chemistry280(47)39515-23(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPPL2b is targeted through the secretory pathway to endosomes/lysosomes, whereas SPP and SPPL3 are restricted to the ER.
Title: Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.

The following sppl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sppl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39849	NM_001045244.2 Latest version!	Danio rerio signal peptide peptidase-like 2 (sppl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa150273	XM_021478906.1 Latest version!	Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa150274	XM_021478911.1 Latest version!	Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sppl2 ( NM_001045244.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038709.2 Danio rerio signal peptide peptidase-like 2 (sppl2), mRNA.

Price & Availability↑

CloneID	ODa39849
Clone ID Related Accession (Same CDS sequence)	NM_001045244.2
Accession Version	NM_001045244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	signal peptide peptidase-like 2B isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX539340.7, EH550022.1 and EH571140.1. On Jun 17, 2015 this sequence version replaced NM_001045244.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC044512.1, GFIL01039541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGTCTG 
CTGGTAGCTT ACTGTCACAG ATTTGATATC CTCATGCAGA CTTCTCAAAT ATATTTCCTG 
GCCTGCACTA TCGGGTACGG CATTGGTCTT CTCATCACGT TTGTGGCTCT GACTCTGATG 
CAGATGGGTC AGCCTGCCCT CTTATACCTG GTGCCTTGCA CACTTCTTAC CAGCCTTGCA 
GTGGCACTGT GGCGCAAGGA GCTGCCGTTG TTCTGGACGG GAAGTGGATT TGTGCCTCCT 
CCTATAATCA TGGAACAAAT CAACTGTACT CAAACCCCAG GACCGTCCAC AGATGAACCC 
CCATCCAATC CAGAACCGGC AACCTCTGAA ACCAACAATC AACGCGAGGA TCCTACTCCA 
GCAACCCAAG AGGAAGTTCC ACATCTTACA GATGCATCTA ATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038709.2
CDS	83..1750
Translation	MRFLSSLLLAALIVEQVLGEYGMAHFSDWGNSKGKDYCIFFNSQWARLPQDLSKATRLQTYDLTSSVLCSPSDVPEGGFPNSIPMVMRGNCTFYEKVRLAQINGAKGLLIVSKDRLTPPSGNKSQYEEIGIPVALLSYKDMLDISKTFGEKRQVAMYAPNEPVVDYNMVLIFLMAVGTVALGGYWAGSRDIKKRYMKHKRDDGAEKQDEETVDVTPIMICVFVVMCCSMLVLLYFFYDQLVYMTIATFCLASAVSLYSCLWPFVRRIPFGKCRIPENNLPYCHKRPQVRMLILSAFCIGVSVTWGVFRNEDQWAWILQDALGIAFCLYMLKTIRLPTFKACTLLLVVLFVYDVFFVFITPLLTKSGESIMVEVAAGPSDSSTHEKLPMVLKVPRLNSSPLVLCDRPFSLLGFGDILVPGLLVAYCHRFDILMQTSQIYFLACTIGYGIGLLITFVALTLMQMGQPALLYLVPCTLLTSLAVALWRKELPLFWTGSGFVPPPIIMEQINCTQTPGPSTDEPPSNPEPATSETNNQREDPTPATQEEVPHLTDASNT

Target ORF information:

RefSeq Version	NM_001045244.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide peptidase-like 2 (sppl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045244.2

ORF Insert Sequence:

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGTCTG 
CTGGTAGCTT ACTGTCACAG ATTTGATATC CTCATGCAGA CTTCTCAAAT ATATTTCCTG 
GCCTGCACTA TCGGGTACGG CATTGGTCTT CTCATCACGT TTGTGGCTCT GACTCTGATG 
CAGATGGGTC AGCCTGCCCT CTTATACCTG GTGCCTTGCA CACTTCTTAC CAGCCTTGCA 
GTGGCACTGT GGCGCAAGGA GCTGCCGTTG TTCTGGACGG GAAGTGGATT TGTGCCTCCT 
CCTATAATCA TGGAACAAAT CAACTGTACT CAAACCCCAG GACCGTCCAC AGATGAACCC 
CCATCCAATC CAGAACCGGC AACCTCTGAA ACCAACAATC AACGCGAGGA TCCTACTCCA 
GCAACCCAAG AGGAAGTTCC ACATCTTACA GATGCATCTA ATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2 ( XM_021478906.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334581.1 Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150273
Clone ID Related Accession (Same CDS sequence)	XM_021478906.1
Accession Version	XM_021478906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	signal peptide peptidase-like 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGGTAC 
GGCATTGGTC TTCTCATCAC GTTTGTGGCT CTGACTCTGA TGCAGATGGG TCAGCCTGCC 
CTCTTATACC TGGTGCCTTG CACACTTCTT ACCAGCCTTG CAGTGGCACT GTGGCGCAAG 
GAGCTGCCGT TGTTCTGGAC GGGAAGTGGA TTTGTGCCTC CTCCTATAAT CATGGAACAA 
ATCAACTGTA CTCAAACCCC AGGACCGTCC ACAGATGAAC CCCCATCCAA TCCAGAACCG 
GCAACCTCTG AAACCAACAA TCAACGCGAG GATCCTACTC CAGCAACCCA AGAGGAAGTT 
CCACATCTTA CAGATGCATC TAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334581.1
CDS	78..1667
Translation	MRFLSSLLLAALIVEQVLGEYGMAHFSDWGNSKGKDYCIFFNSQWARLPQDLSKATRLQTYDLTSSVLCSPSDVPEGGFPNSIPMVMRGNCTFYEKVRLAQINGAKGLLIVSKDRLTPPSGNKSQYEEIGIPVALLSYKDMLDISKTFGEKRQVAMYAPNEPVVDYNMVLIFLMAVGTVALGGYWAGSRDIKKRYMKHKRDDGAEKQDEETVDVTPIMICVFVVMCCSMLVLLYFFYDQLVYMTIATFCLASAVSLYSCLWPFVRRIPFGKCRIPENNLPYCHKRPQVRMLILSAFCIGVSVTWGVFRNEDQWAWILQDALGIAFCLYMLKTIRLPTFKACTLLLVVLFVYDVFFVFITPLLTKSGESIMVEVAAGPSDSSTHEKLPMVLKVPRLNSSPLVLCDRPFSLLGFGDILVPGYGIGLLITFVALTLMQMGQPALLYLVPCTLLTSLAVALWRKELPLFWTGSGFVPPPIIMEQINCTQTPGPSTDEPPSNPEPATSETNNQREDPTPATQEEVPHLTDASNT

Target ORF information:

RefSeq Version	XM_021478906.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478906.1

ORF Insert Sequence:

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGGTAC 
GGCATTGGTC TTCTCATCAC GTTTGTGGCT CTGACTCTGA TGCAGATGGG TCAGCCTGCC 
CTCTTATACC TGGTGCCTTG CACACTTCTT ACCAGCCTTG CAGTGGCACT GTGGCGCAAG 
GAGCTGCCGT TGTTCTGGAC GGGAAGTGGA TTTGTGCCTC CTCCTATAAT CATGGAACAA 
ATCAACTGTA CTCAAACCCC AGGACCGTCC ACAGATGAAC CCCCATCCAA TCCAGAACCG 
GCAACCTCTG AAACCAACAA TCAACGCGAG GATCCTACTC CAGCAACCCA AGAGGAAGTT 
CCACATCTTA CAGATGCATC TAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sppl2 ( XM_021478911.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334586.1 Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150274
Clone ID Related Accession (Same CDS sequence)	XM_021478911.1
Accession Version	XM_021478911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	signal peptide peptidase-like 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGTCTG 
CTGGTAGCTT ACTGTCACAG ATTTGATATC CTCATGCAGA CTTCTCAAAT ATATTTCCTG 
GCCTGCACTA TCGGGTACGG CATTGGTCTT CTCATCACGT TTGTGGCTCT GACTCTGATG 
CAGATGGGTC AGCCTGCCCT CTTATACCTG GTGCCTTGCA CACTTCTTAC CAGCCTTGCA 
GTGGCACTGT GGCGCAAGGA GCTGCCGTTG TTCTGGACGG GAAGTGGATT TGTGGTGAAT 
ACCAGTTTGA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334586.1
CDS	78..1592
Translation	MRFLSSLLLAALIVEQVLGEYGMAHFSDWGNSKGKDYCIFFNSQWARLPQDLSKATRLQTYDLTSSVLCSPSDVPEGGFPNSIPMVMRGNCTFYEKVRLAQINGAKGLLIVSKDRLTPPSGNKSQYEEIGIPVALLSYKDMLDISKTFGEKRQVAMYAPNEPVVDYNMVLIFLMAVGTVALGGYWAGSRDIKKRYMKHKRDDGAEKQDEETVDVTPIMICVFVVMCCSMLVLLYFFYDQLVYMTIATFCLASAVSLYSCLWPFVRRIPFGKCRIPENNLPYCHKRPQVRMLILSAFCIGVSVTWGVFRNEDQWAWILQDALGIAFCLYMLKTIRLPTFKACTLLLVVLFVYDVFFVFITPLLTKSGESIMVEVAAGPSDSSTHEKLPMVLKVPRLNSSPLVLCDRPFSLLGFGDILVPGLLVAYCHRFDILMQTSQIYFLACTIGYGIGLLITFVALTLMQMGQPALLYLVPCTLLTSLAVALWRKELPLFWTGSGFVVNTSLI

Target ORF information:

RefSeq Version	XM_021478911.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide peptidase-like 2 (sppl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478911.1

ORF Insert Sequence:

ATGAGATTTT TGTCCAGTCT CCTTTTGGCA GCTCTTATAG TCGAACAGGT TTTAGGTGAA 
TATGGCATGG CTCATTTCAG CGATTGGGGG AACAGTAAGG GCAAAGATTA CTGCATATTC 
TTCAACTCTC AATGGGCTCG ACTACCTCAG GACCTCAGTA AAGCCACACG GCTGCAGACC 
TATGATCTGA CCAGTTCAGT GCTGTGCTCG CCCTCTGATG TACCTGAGGG TGGCTTCCCA 
AACAGCATCC CAATGGTGAT GAGAGGAAAT TGTACTTTCT ATGAAAAAGT CAGACTGGCC 
CAGATCAATG GTGCCAAGGG ACTTCTGATT GTCAGCAAAG ACAGGCTGAC TCCACCCTCA 
GGCAATAAAT CCCAGTATGA GGAGATTGGC ATCCCAGTGG CTTTGCTGAG CTATAAAGAC 
ATGCTGGATA TCAGTAAGAC ATTTGGGGAG AAAAGGCAGG TGGCCATGTA TGCGCCCAAT 
GAACCGGTGG TGGACTACAA CATGGTGCTG ATATTCCTGA TGGCAGTGGG CACGGTGGCT 
TTAGGAGGAT ACTGGGCTGG CAGCAGGGAC ATTAAAAAAC GCTACATGAA GCACAAGAGG 
GACGATGGTG CTGAGAAACA AGACGAGGAG ACCGTTGACG TCACCCCCAT CATGATTTGT 
GTGTTTGTGG TCATGTGCTG CTCCATGCTG GTGCTCCTGT ATTTCTTCTA CGATCAACTG 
GTGTATATGA CCATAGCTAC ATTCTGTCTG GCGTCTGCAG TCAGCCTGTA CAGCTGTCTT 
TGGCCCTTTG TCCGAAGAAT ACCATTTGGG AAATGCAGGA TTCCTGAGAA TAACCTTCCA 
TATTGTCACA AGCGGCCACA GGTGCGCATG TTGATCCTTT CGGCCTTCTG CATAGGAGTC 
AGCGTAACTT GGGGAGTGTT CCGCAATGAA GACCAGTGGG CATGGATACT TCAGGATGCA 
TTAGGCATCG CATTCTGTCT CTACATGCTG AAAACAATCC GGCTACCCAC TTTCAAGGCC 
TGCACTCTTC TGCTGGTCGT GCTTTTCGTT TATGACGTGT TTTTTGTTTT CATAACACCA 
CTTCTGACTA AGAGTGGGGA AAGCATAATG GTAGAGGTGG CGGCTGGTCC CTCTGATTCC 
TCTACCCATG AGAAGCTTCC CATGGTGCTG AAAGTGCCCA GGTTAAATTC GTCTCCTCTA 
GTCCTGTGTG ACCGGCCCTT CTCTCTTCTT GGCTTTGGTG ATATCTTGGT GCCAGGTCTG 
CTGGTAGCTT ACTGTCACAG ATTTGATATC CTCATGCAGA CTTCTCAAAT ATATTTCCTG 
GCCTGCACTA TCGGGTACGG CATTGGTCTT CTCATCACGT TTGTGGCTCT GACTCTGATG 
CAGATGGGTC AGCCTGCCCT CTTATACCTG GTGCCTTGCA CACTTCTTAC CAGCCTTGCA 
GTGGCACTGT GGCGCAAGGA GCTGCCGTTG TTCTGGACGG GAAGTGGATT TGTGGTGAAT 
ACCAGTTTGA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Cellular functions of gamma-secretase-related proteins.
Neuro-degenerative diseases3(4-5)284-9(2006)
Haffner C,Haass C

Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.
The Journal of biological chemistry280(47)39515-23(2005 Nov)
Krawitz P,Haffner C,Fluhrer R,Steiner H,Schmid B,Haass C

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sppl2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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