map3k4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k4
Entrez Gene ID	325488
Full Name	mitogen-activated protein kinase kinase kinase 4
Synonyms	cb135,mekk4,wu:fa02f11,wu:fc83c01
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase 4 MAP/ERK kinase kinase 4 fa02f11
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307494.3	XP_009305769.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_009307496.3	XP_009305771.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_009307494.2	XP_009305769.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_009307495.2	XP_009305770.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_021480882.1	XP_021336557.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_009307496.2	XP_009305771.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_009307495.3	XP_009305770.1	mitogen-activated protein kinase kinase kinase 4 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Map3k4	NP_036078.2
Gallus gallus (chicken)	MAP3K4	XP_004935618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002371	XP_312585.5
	MAP3K4	XP_001098969.2
Canis lupus familiaris (dog)	MAP3K4	XP_005615756.1
Danio rerio (zebrafish)	map3k4	XP_005157074.1
Xenopus tropicalis (tropical clawed frog)	map3k4	XP_004914705.1
Pan troglodytes (chimpanzee)	MAP3K4	XP_003311625.2
Bos taurus (cattle)	MAP3K4	XP_002690423.2
Rattus norvegicus (Norway rat)	Map3k4	NP_001100926.1
Drosophila melanogaster (fruit fly)	Mekk1	NP_650750.4
Homo sapiens (human)	MAP3K4	NP_005913.2

Gene Ontology

The following map3k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148650	XM_021480882.1 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19007	XM_009307494.3 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19008	XM_009307495.3 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19009	XM_009307496.3 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa19007	XM_009307494.2	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19009	XM_009307496.2	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa19008	XM_009307495.2	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k4 ( XM_021480882.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336557.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148650
Clone ID Related Accession (Same CDS sequence)	XM_021480882.1
Accession Version	XM_021480882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGAG TCTCCACTGG ACATGAGAAT GACCGTCTGT CGTCTGTGGC TGCGGAGCTG 
CAGTTTAAAT CCCTCAGTCG ACACTCCAGC CCAACGGAGG ACAGGGAAGA GCCGTCGTAT 
CCCAAAGCAG ACCCCAGCAG TTCAGCCCGT CGCAGCTGGG AGCTTCGCAA CTTCATCAGC 
CAGTCTAAAG ACACAGCGCG TCAGAGCCCC CTGGAGGCAG TGCGGCGCTC CATACAAACC 
TTCGATGAGA AACACTATGC GCTTATGAGA CAGAGAAACA TCATTGGACA GGTTTGCAAC 
ACGCCGAAGT CCTACGATAA CGTGATGCAT GTGGGCCTGC GCAAAGTCAC CTTTAAATGG 
CAGAGAGGAA ACAAGATCGG TGAAGGGCAG TATGGGAAGG TTTACACATG CATCAATGTG 
GACACTGGTG AACTAATGGC CATGAAGGAG ATTCGCTTTC AGCCTAATGA CCACAAAACC 
ATCAAGGAAA CGGCCGATGA GCTGAAGATC TTCGAAGGAA TCAAACATCC CAACCTCGTC 
CGCTACTTCG GTGTTGAGTT GCACAGGGAG GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAAGGCACGC TGGAGGAAGT GTCCCGTCTG GGCCTCCAGG AACACGTCAT TCGCCTCTAC 
AGCAAACAGA TCACCACGGC CATCAATGTC CTGCATGAAC ACGGCATCGT CCACCGAGAC 
ATTAAAGGAG CGAATATCTT CCTCACGTCT TCGGGTCTGA TAAAGCTGGG GGATTTTGGC 
TGCTCAGTGA AGCTGAAAAA CAACGCTCAG ACGATGCCTG GAGAGGTGAA CAGCACTCTG 
GGAACAGCAG CATACATGGC TCCTGAGGTG ATCACCAGAG CGAAGGGTGA AGGTCACGGT 
CGAGCTGCTG ATATCTGGAG TCTCGGTTGT GTTCTCATAG AAATGGTCAC TGGAAAGAGA 
CCTTGGCACG AGTACGAGCA TAACTTCCAG ATCATGTATA GAGTGGGAAT GGGCCACAAA 
CCGCCCATTC CTGAAAAGCT GAGCACAGAG GGGAAGGATT TTCTGGCTCA CTGTCTGGAG 
AGCGAACCCA AGCGGCGCTG GACCGCCAGT GCCCTGCTGG ACCACCCTTT TGTCAAGGTC 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336557.1
CDS	356..4873
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_021480882.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480882.1

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGAG TCTCCACTGG ACATGAGAAT GACCGTCTGT CGTCTGTGGC TGCGGAGCTG 
CAGTTTAAAT CCCTCAGTCG ACACTCCAGC CCAACGGAGG ACAGGGAAGA GCCGTCGTAT 
CCCAAAGCAG ACCCCAGCAG TTCAGCCCGT CGCAGCTGGG AGCTTCGCAA CTTCATCAGC 
CAGTCTAAAG ACACAGCGCG TCAGAGCCCC CTGGAGGCAG TGCGGCGCTC CATACAAACC 
TTCGATGAGA AACACTATGC GCTTATGAGA CAGAGAAACA TCATTGGACA GGTTTGCAAC 
ACGCCGAAGT CCTACGATAA CGTGATGCAT GTGGGCCTGC GCAAAGTCAC CTTTAAATGG 
CAGAGAGGAA ACAAGATCGG TGAAGGGCAG TATGGGAAGG TTTACACATG CATCAATGTG 
GACACTGGTG AACTAATGGC CATGAAGGAG ATTCGCTTTC AGCCTAATGA CCACAAAACC 
ATCAAGGAAA CGGCCGATGA GCTGAAGATC TTCGAAGGAA TCAAACATCC CAACCTCGTC 
CGCTACTTCG GTGTTGAGTT GCACAGGGAG GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAAGGCACGC TGGAGGAAGT GTCCCGTCTG GGCCTCCAGG AACACGTCAT TCGCCTCTAC 
AGCAAACAGA TCACCACGGC CATCAATGTC CTGCATGAAC ACGGCATCGT CCACCGAGAC 
ATTAAAGGAG CGAATATCTT CCTCACGTCT TCGGGTCTGA TAAAGCTGGG GGATTTTGGC 
TGCTCAGTGA AGCTGAAAAA CAACGCTCAG ACGATGCCTG GAGAGGTGAA CAGCACTCTG 
GGAACAGCAG CATACATGGC TCCTGAGGTG ATCACCAGAG CGAAGGGTGA AGGTCACGGT 
CGAGCTGCTG ATATCTGGAG TCTCGGTTGT GTTCTCATAG AAATGGTCAC TGGAAAGAGA 
CCTTGGCACG AGTACGAGCA TAACTTCCAG ATCATGTATA GAGTGGGAAT GGGCCACAAA 
CCGCCCATTC CTGAAAAGCT GAGCACAGAG GGGAAGGATT TTCTGGCTCA CTGTCTGGAG 
AGCGAACCCA AGCGGCGCTG GACCGCCAGT GCCCTGCTGG ACCACCCTTT TGTCAAGGTC 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307494.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305769.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19007
Clone ID Related Accession (Same CDS sequence)	XM_009307494.3 , XM_009307494.2
Accession Version	XM_009307494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GCTCTCGGAG TCTGCCCTGT GATCTGCGTA CCCAGTTGTG TGTCCCAGGT 
CCCCGTCCCA CAGGGGAACA CGGCCACGCC AACAAACCCT CGAATGACAT CAGAGTCTCC 
ACTGGACATG AGAATGACCG TCTGTCGTCT GTGGCTGCGG AGCTGCAGTT TAAATCCCTC 
AGTCGACACT CCAGCCCAAC GGAGGACAGG GAAGAGCCGT CGTATCCCAA AGCAGACCCC 
AGCAGTTCAG CCCGTCGCAG CTGGGAGCTT CGCAACTTCA TCAGCCAGTC TAAAGACACA 
GCGCGTCAGA GCCCCCTGGA GGCAGTGCGG CGCTCCATAC AAACCTTCGA TGAGAAACAC 
TATGCGCTTA TGAGACAGAG AAACATCATT GGACAGGTTT GCAACACGCC GAAGTCCTAC 
GATAACGTGA TGCATGTGGG CCTGCGCAAA GTCACCTTTA AATGGCAGAG AGGAAACAAG 
ATCGGTGAAG GGCAGTATGG GAAGGTTTAC ACATGCATCA ATGTGGACAC TGGTGAACTA 
ATGGCCATGA AGGAGATTCG CTTTCAGCCT AATGACCACA AAACCATCAA GGAAACGGCC 
GATGAGCTGA AGATCTTCGA AGGAATCAAA CATCCCAACC TCGTCCGCTA CTTCGGTGTT 
GAGTTGCACA GGGAGGAGAT GTACATCTTC ATGGAGTACT GTGATGAAGG CACGCTGGAG 
GAAGTGTCCC GTCTGGGCCT CCAGGAACAC GTCATTCGCC TCTACAGCAA ACAGATCACC 
ACGGCCATCA ATGTCCTGCA TGAACACGGC ATCGTCCACC GAGACATTAA AGGAGCGAAT 
ATCTTCCTCA CGTCTTCGGG TCTGATAAAG CTGGGGGATT TTGGCTGCTC AGTGAAGCTG 
AAAAACAACG CTCAGACGAT GCCTGGAGAG GTGAACAGCA CTCTGGGAAC AGCAGCATAC 
ATGGCTCCTG AGGTGATCAC CAGAGCGAAG GGTGAAGGTC ACGGTCGAGC TGCTGATATC 
TGGAGTCTCG GTTGTGTTCT CATAGAAATG GTCACTGGAA AGAGACCTTG GCACGAGTAC 
GAGCATAACT TCCAGATCAT GTATAGAGTG GGAATGGGCC ACAAACCGCC CATTCCTGAA 
AAGCTGAGCA CAGAGGGGAA GGATTTTCTG GCTCACTGTC TGGAGAGCGA ACCCAAGCGG 
CGCTGGACCG CCAGTGCCCT GCTGGACCAC CCTTTTGTCA AGGTCTGCAC AGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305769.1
CDS	355..4977
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFAGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFSSRSLPCDLRTQLCVPGPRPTGEHGHANKPSNDIRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307494.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307494.3

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GCTCTCGGAG TCTGCCCTGT GATCTGCGTA CCCAGTTGTG TGTCCCAGGT 
CCCCGTCCCA CAGGGGAACA CGGCCACGCC AACAAACCCT CGAATGACAT CAGAGTCTCC 
ACTGGACATG AGAATGACCG TCTGTCGTCT GTGGCTGCGG AGCTGCAGTT TAAATCCCTC 
AGTCGACACT CCAGCCCAAC GGAGGACAGG GAAGAGCCGT CGTATCCCAA AGCAGACCCC 
AGCAGTTCAG CCCGTCGCAG CTGGGAGCTT CGCAACTTCA TCAGCCAGTC TAAAGACACA 
GCGCGTCAGA GCCCCCTGGA GGCAGTGCGG CGCTCCATAC AAACCTTCGA TGAGAAACAC 
TATGCGCTTA TGAGACAGAG AAACATCATT GGACAGGTTT GCAACACGCC GAAGTCCTAC 
GATAACGTGA TGCATGTGGG CCTGCGCAAA GTCACCTTTA AATGGCAGAG AGGAAACAAG 
ATCGGTGAAG GGCAGTATGG GAAGGTTTAC ACATGCATCA ATGTGGACAC TGGTGAACTA 
ATGGCCATGA AGGAGATTCG CTTTCAGCCT AATGACCACA AAACCATCAA GGAAACGGCC 
GATGAGCTGA AGATCTTCGA AGGAATCAAA CATCCCAACC TCGTCCGCTA CTTCGGTGTT 
GAGTTGCACA GGGAGGAGAT GTACATCTTC ATGGAGTACT GTGATGAAGG CACGCTGGAG 
GAAGTGTCCC GTCTGGGCCT CCAGGAACAC GTCATTCGCC TCTACAGCAA ACAGATCACC 
ACGGCCATCA ATGTCCTGCA TGAACACGGC ATCGTCCACC GAGACATTAA AGGAGCGAAT 
ATCTTCCTCA CGTCTTCGGG TCTGATAAAG CTGGGGGATT TTGGCTGCTC AGTGAAGCTG 
AAAAACAACG CTCAGACGAT GCCTGGAGAG GTGAACAGCA CTCTGGGAAC AGCAGCATAC 
ATGGCTCCTG AGGTGATCAC CAGAGCGAAG GGTGAAGGTC ACGGTCGAGC TGCTGATATC 
TGGAGTCTCG GTTGTGTTCT CATAGAAATG GTCACTGGAA AGAGACCTTG GCACGAGTAC 
GAGCATAACT TCCAGATCAT GTATAGAGTG GGAATGGGCC ACAAACCGCC CATTCCTGAA 
AAGCTGAGCA CAGAGGGGAA GGATTTTCTG GCTCACTGTC TGGAGAGCGA ACCCAAGCGG 
CGCTGGACCG CCAGTGCCCT GCTGGACCAC CCTTTTGTCA AGGTCTGCAC AGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307494.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305769.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

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CloneID	ODa19007
Clone ID Related Accession (Same CDS sequence)	XM_009307494.3 , XM_009307494.2
Accession Version	XM_009307494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GCTCTCGGAG TCTGCCCTGT GATCTGCGTA CCCAGTTGTG TGTCCCAGGT 
CCCCGTCCCA CAGGGGAACA CGGCCACGCC AACAAACCCT CGAATGACAT CAGAGTCTCC 
ACTGGACATG AGAATGACCG TCTGTCGTCT GTGGCTGCGG AGCTGCAGTT TAAATCCCTC 
AGTCGACACT CCAGCCCAAC GGAGGACAGG GAAGAGCCGT CGTATCCCAA AGCAGACCCC 
AGCAGTTCAG CCCGTCGCAG CTGGGAGCTT CGCAACTTCA TCAGCCAGTC TAAAGACACA 
GCGCGTCAGA GCCCCCTGGA GGCAGTGCGG CGCTCCATAC AAACCTTCGA TGAGAAACAC 
TATGCGCTTA TGAGACAGAG AAACATCATT GGACAGGTTT GCAACACGCC GAAGTCCTAC 
GATAACGTGA TGCATGTGGG CCTGCGCAAA GTCACCTTTA AATGGCAGAG AGGAAACAAG 
ATCGGTGAAG GGCAGTATGG GAAGGTTTAC ACATGCATCA ATGTGGACAC TGGTGAACTA 
ATGGCCATGA AGGAGATTCG CTTTCAGCCT AATGACCACA AAACCATCAA GGAAACGGCC 
GATGAGCTGA AGATCTTCGA AGGAATCAAA CATCCCAACC TCGTCCGCTA CTTCGGTGTT 
GAGTTGCACA GGGAGGAGAT GTACATCTTC ATGGAGTACT GTGATGAAGG CACGCTGGAG 
GAAGTGTCCC GTCTGGGCCT CCAGGAACAC GTCATTCGCC TCTACAGCAA ACAGATCACC 
ACGGCCATCA ATGTCCTGCA TGAACACGGC ATCGTCCACC GAGACATTAA AGGAGCGAAT 
ATCTTCCTCA CGTCTTCGGG TCTGATAAAG CTGGGGGATT TTGGCTGCTC AGTGAAGCTG 
AAAAACAACG CTCAGACGAT GCCTGGAGAG GTGAACAGCA CTCTGGGAAC AGCAGCATAC 
ATGGCTCCTG AGGTGATCAC CAGAGCGAAG GGTGAAGGTC ACGGTCGAGC TGCTGATATC 
TGGAGTCTCG GTTGTGTTCT CATAGAAATG GTCACTGGAA AGAGACCTTG GCACGAGTAC 
GAGCATAACT TCCAGATCAT GTATAGAGTG GGAATGGGCC ACAAACCGCC CATTCCTGAA 
AAGCTGAGCA CAGAGGGGAA GGATTTTCTG GCTCACTGTC TGGAGAGCGA ACCCAAGCGG 
CGCTGGACCG CCAGTGCCCT GCTGGACCAC CCTTTTGTCA AGGTCTGCAC AGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305769.1
CDS	669..5291
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFAGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFSSRSLPCDLRTQLCVPGPRPTGEHGHANKPSNDIRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307494.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307494.2

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GCTCTCGGAG TCTGCCCTGT GATCTGCGTA CCCAGTTGTG TGTCCCAGGT 
CCCCGTCCCA CAGGGGAACA CGGCCACGCC AACAAACCCT CGAATGACAT CAGAGTCTCC 
ACTGGACATG AGAATGACCG TCTGTCGTCT GTGGCTGCGG AGCTGCAGTT TAAATCCCTC 
AGTCGACACT CCAGCCCAAC GGAGGACAGG GAAGAGCCGT CGTATCCCAA AGCAGACCCC 
AGCAGTTCAG CCCGTCGCAG CTGGGAGCTT CGCAACTTCA TCAGCCAGTC TAAAGACACA 
GCGCGTCAGA GCCCCCTGGA GGCAGTGCGG CGCTCCATAC AAACCTTCGA TGAGAAACAC 
TATGCGCTTA TGAGACAGAG AAACATCATT GGACAGGTTT GCAACACGCC GAAGTCCTAC 
GATAACGTGA TGCATGTGGG CCTGCGCAAA GTCACCTTTA AATGGCAGAG AGGAAACAAG 
ATCGGTGAAG GGCAGTATGG GAAGGTTTAC ACATGCATCA ATGTGGACAC TGGTGAACTA 
ATGGCCATGA AGGAGATTCG CTTTCAGCCT AATGACCACA AAACCATCAA GGAAACGGCC 
GATGAGCTGA AGATCTTCGA AGGAATCAAA CATCCCAACC TCGTCCGCTA CTTCGGTGTT 
GAGTTGCACA GGGAGGAGAT GTACATCTTC ATGGAGTACT GTGATGAAGG CACGCTGGAG 
GAAGTGTCCC GTCTGGGCCT CCAGGAACAC GTCATTCGCC TCTACAGCAA ACAGATCACC 
ACGGCCATCA ATGTCCTGCA TGAACACGGC ATCGTCCACC GAGACATTAA AGGAGCGAAT 
ATCTTCCTCA CGTCTTCGGG TCTGATAAAG CTGGGGGATT TTGGCTGCTC AGTGAAGCTG 
AAAAACAACG CTCAGACGAT GCCTGGAGAG GTGAACAGCA CTCTGGGAAC AGCAGCATAC 
ATGGCTCCTG AGGTGATCAC CAGAGCGAAG GGTGAAGGTC ACGGTCGAGC TGCTGATATC 
TGGAGTCTCG GTTGTGTTCT CATAGAAATG GTCACTGGAA AGAGACCTTG GCACGAGTAC 
GAGCATAACT TCCAGATCAT GTATAGAGTG GGAATGGGCC ACAAACCGCC CATTCCTGAA 
AAGCTGAGCA CAGAGGGGAA GGATTTTCTG GCTCACTGTC TGGAGAGCGA ACCCAAGCGG 
CGCTGGACCG CCAGTGCCCT GCTGGACCAC CCTTTTGTCA AGGTCTGCAC AGATGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307495.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305770.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

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CloneID	ODa19008
Clone ID Related Accession (Same CDS sequence)	XM_009307495.2 , XM_009307495.3
Accession Version	XM_009307495.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGCT CTCGGAGTCT GCCCTGTGAT CTGCGTACCC AGTTGTGTGT CCCAGGTCCC 
CGTCCCACAG GGGAACACGG CCACGCCAAC AAACCCTCGA ATGACATCAG AGTCTCCACT 
GGACATGAGA ATGACCGTCT GTCGTCTGTG GCTGCGGAGC TGCAGTTTAA ATCCCTCAGT 
CGACACTCCA GCCCAACGGA GGACAGGGAA GAGCCGTCGT ATCCCAAAGC AGACCCCAGC 
AGTTCAGCCC GTCGCAGCTG GGAGCTTCGC AACTTCATCA GCCAGTCTAA AGACACAGCG 
CGTCAGAGCC CCCTGGAGGC AGTGCGGCGC TCCATACAAA CCTTCGATGA GAAACACTAT 
GCGCTTATGA GACAGAGAAA CATCATTGGA CAGGTTTGCA ACACGCCGAA GTCCTACGAT 
AACGTGATGC ATGTGGGCCT GCGCAAAGTC ACCTTTAAAT GGCAGAGAGG AAACAAGATC 
GGTGAAGGGC AGTATGGGAA GGTTTACACA TGCATCAATG TGGACACTGG TGAACTAATG 
GCCATGAAGG AGATTCGCTT TCAGCCTAAT GACCACAAAA CCATCAAGGA AACGGCCGAT 
GAGCTGAAGA TCTTCGAAGG AATCAAACAT CCCAACCTCG TCCGCTACTT CGGTGTTGAG 
TTGCACAGGG AGGAGATGTA CATCTTCATG GAGTACTGTG ATGAAGGCAC GCTGGAGGAA 
GTGTCCCGTC TGGGCCTCCA GGAACACGTC ATTCGCCTCT ACAGCAAACA GATCACCACG 
GCCATCAATG TCCTGCATGA ACACGGCATC GTCCACCGAG ACATTAAAGG AGCGAATATC 
TTCCTCACGT CTTCGGGTCT GATAAAGCTG GGGGATTTTG GCTGCTCAGT GAAGCTGAAA 
AACAACGCTC AGACGATGCC TGGAGAGGTG AACAGCACTC TGGGAACAGC AGCATACATG 
GCTCCTGAGG TGATCACCAG AGCGAAGGGT GAAGGTCACG GTCGAGCTGC TGATATCTGG 
AGTCTCGGTT GTGTTCTCAT AGAAATGGTC ACTGGAAAGA GACCTTGGCA CGAGTACGAG 
CATAACTTCC AGATCATGTA TAGAGTGGGA ATGGGCCACA AACCGCCCAT TCCTGAAAAG 
CTGAGCACAG AGGGGAAGGA TTTTCTGGCT CACTGTCTGG AGAGCGAACC CAAGCGGCGC 
TGGACCGCCA GTGCCCTGCT GGACCACCCT TTTGTCAAGG TCTGCACAGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305770.1
CDS	670..5289
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFSSRSLPCDLRTQLCVPGPRPTGEHGHANKPSNDIRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307495.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307495.2

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGCT CTCGGAGTCT GCCCTGTGAT CTGCGTACCC AGTTGTGTGT CCCAGGTCCC 
CGTCCCACAG GGGAACACGG CCACGCCAAC AAACCCTCGA ATGACATCAG AGTCTCCACT 
GGACATGAGA ATGACCGTCT GTCGTCTGTG GCTGCGGAGC TGCAGTTTAA ATCCCTCAGT 
CGACACTCCA GCCCAACGGA GGACAGGGAA GAGCCGTCGT ATCCCAAAGC AGACCCCAGC 
AGTTCAGCCC GTCGCAGCTG GGAGCTTCGC AACTTCATCA GCCAGTCTAA AGACACAGCG 
CGTCAGAGCC CCCTGGAGGC AGTGCGGCGC TCCATACAAA CCTTCGATGA GAAACACTAT 
GCGCTTATGA GACAGAGAAA CATCATTGGA CAGGTTTGCA ACACGCCGAA GTCCTACGAT 
AACGTGATGC ATGTGGGCCT GCGCAAAGTC ACCTTTAAAT GGCAGAGAGG AAACAAGATC 
GGTGAAGGGC AGTATGGGAA GGTTTACACA TGCATCAATG TGGACACTGG TGAACTAATG 
GCCATGAAGG AGATTCGCTT TCAGCCTAAT GACCACAAAA CCATCAAGGA AACGGCCGAT 
GAGCTGAAGA TCTTCGAAGG AATCAAACAT CCCAACCTCG TCCGCTACTT CGGTGTTGAG 
TTGCACAGGG AGGAGATGTA CATCTTCATG GAGTACTGTG ATGAAGGCAC GCTGGAGGAA 
GTGTCCCGTC TGGGCCTCCA GGAACACGTC ATTCGCCTCT ACAGCAAACA GATCACCACG 
GCCATCAATG TCCTGCATGA ACACGGCATC GTCCACCGAG ACATTAAAGG AGCGAATATC 
TTCCTCACGT CTTCGGGTCT GATAAAGCTG GGGGATTTTG GCTGCTCAGT GAAGCTGAAA 
AACAACGCTC AGACGATGCC TGGAGAGGTG AACAGCACTC TGGGAACAGC AGCATACATG 
GCTCCTGAGG TGATCACCAG AGCGAAGGGT GAAGGTCACG GTCGAGCTGC TGATATCTGG 
AGTCTCGGTT GTGTTCTCAT AGAAATGGTC ACTGGAAAGA GACCTTGGCA CGAGTACGAG 
CATAACTTCC AGATCATGTA TAGAGTGGGA ATGGGCCACA AACCGCCCAT TCCTGAAAAG 
CTGAGCACAG AGGGGAAGGA TTTTCTGGCT CACTGTCTGG AGAGCGAACC CAAGCGGCGC 
TGGACCGCCA GTGCCCTGCT GGACCACCCT TTTGTCAAGG TCTGCACAGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307495.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305770.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

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CloneID	ODa19008
Clone ID Related Accession (Same CDS sequence)	XM_009307495.2 , XM_009307495.3
Accession Version	XM_009307495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGCT CTCGGAGTCT GCCCTGTGAT CTGCGTACCC AGTTGTGTGT CCCAGGTCCC 
CGTCCCACAG GGGAACACGG CCACGCCAAC AAACCCTCGA ATGACATCAG AGTCTCCACT 
GGACATGAGA ATGACCGTCT GTCGTCTGTG GCTGCGGAGC TGCAGTTTAA ATCCCTCAGT 
CGACACTCCA GCCCAACGGA GGACAGGGAA GAGCCGTCGT ATCCCAAAGC AGACCCCAGC 
AGTTCAGCCC GTCGCAGCTG GGAGCTTCGC AACTTCATCA GCCAGTCTAA AGACACAGCG 
CGTCAGAGCC CCCTGGAGGC AGTGCGGCGC TCCATACAAA CCTTCGATGA GAAACACTAT 
GCGCTTATGA GACAGAGAAA CATCATTGGA CAGGTTTGCA ACACGCCGAA GTCCTACGAT 
AACGTGATGC ATGTGGGCCT GCGCAAAGTC ACCTTTAAAT GGCAGAGAGG AAACAAGATC 
GGTGAAGGGC AGTATGGGAA GGTTTACACA TGCATCAATG TGGACACTGG TGAACTAATG 
GCCATGAAGG AGATTCGCTT TCAGCCTAAT GACCACAAAA CCATCAAGGA AACGGCCGAT 
GAGCTGAAGA TCTTCGAAGG AATCAAACAT CCCAACCTCG TCCGCTACTT CGGTGTTGAG 
TTGCACAGGG AGGAGATGTA CATCTTCATG GAGTACTGTG ATGAAGGCAC GCTGGAGGAA 
GTGTCCCGTC TGGGCCTCCA GGAACACGTC ATTCGCCTCT ACAGCAAACA GATCACCACG 
GCCATCAATG TCCTGCATGA ACACGGCATC GTCCACCGAG ACATTAAAGG AGCGAATATC 
TTCCTCACGT CTTCGGGTCT GATAAAGCTG GGGGATTTTG GCTGCTCAGT GAAGCTGAAA 
AACAACGCTC AGACGATGCC TGGAGAGGTG AACAGCACTC TGGGAACAGC AGCATACATG 
GCTCCTGAGG TGATCACCAG AGCGAAGGGT GAAGGTCACG GTCGAGCTGC TGATATCTGG 
AGTCTCGGTT GTGTTCTCAT AGAAATGGTC ACTGGAAAGA GACCTTGGCA CGAGTACGAG 
CATAACTTCC AGATCATGTA TAGAGTGGGA ATGGGCCACA AACCGCCCAT TCCTGAAAAG 
CTGAGCACAG AGGGGAAGGA TTTTCTGGCT CACTGTCTGG AGAGCGAACC CAAGCGGCGC 
TGGACCGCCA GTGCCCTGCT GGACCACCCT TTTGTCAAGG TCTGCACAGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305770.1
CDS	357..4976
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFSSRSLPCDLRTQLCVPGPRPTGEHGHANKPSNDIRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307495.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307495.3

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGGC CCACGAAACA GCCCTCGACC TGTGAAAGTC 
CCCCGCTGTC ACAGCGATCC TCCAAACCCG TACCTCTTCA TCCCTTCCAC CCAAGCAGAG 
GGTTTCAGCT CTCGGAGTCT GCCCTGTGAT CTGCGTACCC AGTTGTGTGT CCCAGGTCCC 
CGTCCCACAG GGGAACACGG CCACGCCAAC AAACCCTCGA ATGACATCAG AGTCTCCACT 
GGACATGAGA ATGACCGTCT GTCGTCTGTG GCTGCGGAGC TGCAGTTTAA ATCCCTCAGT 
CGACACTCCA GCCCAACGGA GGACAGGGAA GAGCCGTCGT ATCCCAAAGC AGACCCCAGC 
AGTTCAGCCC GTCGCAGCTG GGAGCTTCGC AACTTCATCA GCCAGTCTAA AGACACAGCG 
CGTCAGAGCC CCCTGGAGGC AGTGCGGCGC TCCATACAAA CCTTCGATGA GAAACACTAT 
GCGCTTATGA GACAGAGAAA CATCATTGGA CAGGTTTGCA ACACGCCGAA GTCCTACGAT 
AACGTGATGC ATGTGGGCCT GCGCAAAGTC ACCTTTAAAT GGCAGAGAGG AAACAAGATC 
GGTGAAGGGC AGTATGGGAA GGTTTACACA TGCATCAATG TGGACACTGG TGAACTAATG 
GCCATGAAGG AGATTCGCTT TCAGCCTAAT GACCACAAAA CCATCAAGGA AACGGCCGAT 
GAGCTGAAGA TCTTCGAAGG AATCAAACAT CCCAACCTCG TCCGCTACTT CGGTGTTGAG 
TTGCACAGGG AGGAGATGTA CATCTTCATG GAGTACTGTG ATGAAGGCAC GCTGGAGGAA 
GTGTCCCGTC TGGGCCTCCA GGAACACGTC ATTCGCCTCT ACAGCAAACA GATCACCACG 
GCCATCAATG TCCTGCATGA ACACGGCATC GTCCACCGAG ACATTAAAGG AGCGAATATC 
TTCCTCACGT CTTCGGGTCT GATAAAGCTG GGGGATTTTG GCTGCTCAGT GAAGCTGAAA 
AACAACGCTC AGACGATGCC TGGAGAGGTG AACAGCACTC TGGGAACAGC AGCATACATG 
GCTCCTGAGG TGATCACCAG AGCGAAGGGT GAAGGTCACG GTCGAGCTGC TGATATCTGG 
AGTCTCGGTT GTGTTCTCAT AGAAATGGTC ACTGGAAAGA GACCTTGGCA CGAGTACGAG 
CATAACTTCC AGATCATGTA TAGAGTGGGA ATGGGCCACA AACCGCCCAT TCCTGAAAAG 
CTGAGCACAG AGGGGAAGGA TTTTCTGGCT CACTGTCTGG AGAGCGAACC CAAGCGGCGC 
TGGACCGCCA GTGCCCTGCT GGACCACCCT TTTGTCAAGG TCTGCACAGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307496.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305771.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa19009
Clone ID Related Accession (Same CDS sequence)	XM_009307496.3 , XM_009307496.2
Accession Version	XM_009307496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GAGTCTCCAC TGGACATGAG AATGACCGTC TGTCGTCTGT GGCTGCGGAG 
CTGCAGTTTA AATCCCTCAG TCGACACTCC AGCCCAACGG AGGACAGGGA AGAGCCGTCG 
TATCCCAAAG CAGACCCCAG CAGTTCAGCC CGTCGCAGCT GGGAGCTTCG CAACTTCATC 
AGCCAGTCTA AAGACACAGC GCGTCAGAGC CCCCTGGAGG CAGTGCGGCG CTCCATACAA 
ACCTTCGATG AGAAACACTA TGCGCTTATG AGACAGAGAA ACATCATTGG ACAGGTTTGC 
AACACGCCGA AGTCCTACGA TAACGTGATG CATGTGGGCC TGCGCAAAGT CACCTTTAAA 
TGGCAGAGAG GAAACAAGAT CGGTGAAGGG CAGTATGGGA AGGTTTACAC ATGCATCAAT 
GTGGACACTG GTGAACTAAT GGCCATGAAG GAGATTCGCT TTCAGCCTAA TGACCACAAA 
ACCATCAAGG AAACGGCCGA TGAGCTGAAG ATCTTCGAAG GAATCAAACA TCCCAACCTC 
GTCCGCTACT TCGGTGTTGA GTTGCACAGG GAGGAGATGT ACATCTTCAT GGAGTACTGT 
GATGAAGGCA CGCTGGAGGA AGTGTCCCGT CTGGGCCTCC AGGAACACGT CATTCGCCTC 
TACAGCAAAC AGATCACCAC GGCCATCAAT GTCCTGCATG AACACGGCAT CGTCCACCGA 
GACATTAAAG GAGCGAATAT CTTCCTCACG TCTTCGGGTC TGATAAAGCT GGGGGATTTT 
GGCTGCTCAG TGAAGCTGAA AAACAACGCT CAGACGATGC CTGGAGAGGT GAACAGCACT 
CTGGGAACAG CAGCATACAT GGCTCCTGAG GTGATCACCA GAGCGAAGGG TGAAGGTCAC 
GGTCGAGCTG CTGATATCTG GAGTCTCGGT TGTGTTCTCA TAGAAATGGT CACTGGAAAG 
AGACCTTGGC ACGAGTACGA GCATAACTTC CAGATCATGT ATAGAGTGGG AATGGGCCAC 
AAACCGCCCA TTCCTGAAAA GCTGAGCACA GAGGGGAAGG ATTTTCTGGC TCACTGTCTG 
GAGAGCGAAC CCAAGCGGCG CTGGACCGCC AGTGCCCTGC TGGACCACCC TTTTGTCAAG 
GTCTGCACAG ATGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305771.1
CDS	354..4874
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFAGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307496.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307496.3

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GAGTCTCCAC TGGACATGAG AATGACCGTC TGTCGTCTGT GGCTGCGGAG 
CTGCAGTTTA AATCCCTCAG TCGACACTCC AGCCCAACGG AGGACAGGGA AGAGCCGTCG 
TATCCCAAAG CAGACCCCAG CAGTTCAGCC CGTCGCAGCT GGGAGCTTCG CAACTTCATC 
AGCCAGTCTA AAGACACAGC GCGTCAGAGC CCCCTGGAGG CAGTGCGGCG CTCCATACAA 
ACCTTCGATG AGAAACACTA TGCGCTTATG AGACAGAGAA ACATCATTGG ACAGGTTTGC 
AACACGCCGA AGTCCTACGA TAACGTGATG CATGTGGGCC TGCGCAAAGT CACCTTTAAA 
TGGCAGAGAG GAAACAAGAT CGGTGAAGGG CAGTATGGGA AGGTTTACAC ATGCATCAAT 
GTGGACACTG GTGAACTAAT GGCCATGAAG GAGATTCGCT TTCAGCCTAA TGACCACAAA 
ACCATCAAGG AAACGGCCGA TGAGCTGAAG ATCTTCGAAG GAATCAAACA TCCCAACCTC 
GTCCGCTACT TCGGTGTTGA GTTGCACAGG GAGGAGATGT ACATCTTCAT GGAGTACTGT 
GATGAAGGCA CGCTGGAGGA AGTGTCCCGT CTGGGCCTCC AGGAACACGT CATTCGCCTC 
TACAGCAAAC AGATCACCAC GGCCATCAAT GTCCTGCATG AACACGGCAT CGTCCACCGA 
GACATTAAAG GAGCGAATAT CTTCCTCACG TCTTCGGGTC TGATAAAGCT GGGGGATTTT 
GGCTGCTCAG TGAAGCTGAA AAACAACGCT CAGACGATGC CTGGAGAGGT GAACAGCACT 
CTGGGAACAG CAGCATACAT GGCTCCTGAG GTGATCACCA GAGCGAAGGG TGAAGGTCAC 
GGTCGAGCTG CTGATATCTG GAGTCTCGGT TGTGTTCTCA TAGAAATGGT CACTGGAAAG 
AGACCTTGGC ACGAGTACGA GCATAACTTC CAGATCATGT ATAGAGTGGG AATGGGCCAC 
AAACCGCCCA TTCCTGAAAA GCTGAGCACA GAGGGGAAGG ATTTTCTGGC TCACTGTCTG 
GAGAGCGAAC CCAAGCGGCG CTGGACCGCC AGTGCCCTGC TGGACCACCC TTTTGTCAAG 
GTCTGCACAG ATGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_009307496.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305771.1 Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa19009
Clone ID Related Accession (Same CDS sequence)	XM_009307496.3 , XM_009307496.2
Accession Version	XM_009307496.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GAGTCTCCAC TGGACATGAG AATGACCGTC TGTCGTCTGT GGCTGCGGAG 
CTGCAGTTTA AATCCCTCAG TCGACACTCC AGCCCAACGG AGGACAGGGA AGAGCCGTCG 
TATCCCAAAG CAGACCCCAG CAGTTCAGCC CGTCGCAGCT GGGAGCTTCG CAACTTCATC 
AGCCAGTCTA AAGACACAGC GCGTCAGAGC CCCCTGGAGG CAGTGCGGCG CTCCATACAA 
ACCTTCGATG AGAAACACTA TGCGCTTATG AGACAGAGAA ACATCATTGG ACAGGTTTGC 
AACACGCCGA AGTCCTACGA TAACGTGATG CATGTGGGCC TGCGCAAAGT CACCTTTAAA 
TGGCAGAGAG GAAACAAGAT CGGTGAAGGG CAGTATGGGA AGGTTTACAC ATGCATCAAT 
GTGGACACTG GTGAACTAAT GGCCATGAAG GAGATTCGCT TTCAGCCTAA TGACCACAAA 
ACCATCAAGG AAACGGCCGA TGAGCTGAAG ATCTTCGAAG GAATCAAACA TCCCAACCTC 
GTCCGCTACT TCGGTGTTGA GTTGCACAGG GAGGAGATGT ACATCTTCAT GGAGTACTGT 
GATGAAGGCA CGCTGGAGGA AGTGTCCCGT CTGGGCCTCC AGGAACACGT CATTCGCCTC 
TACAGCAAAC AGATCACCAC GGCCATCAAT GTCCTGCATG AACACGGCAT CGTCCACCGA 
GACATTAAAG GAGCGAATAT CTTCCTCACG TCTTCGGGTC TGATAAAGCT GGGGGATTTT 
GGCTGCTCAG TGAAGCTGAA AAACAACGCT CAGACGATGC CTGGAGAGGT GAACAGCACT 
CTGGGAACAG CAGCATACAT GGCTCCTGAG GTGATCACCA GAGCGAAGGG TGAAGGTCAC 
GGTCGAGCTG CTGATATCTG GAGTCTCGGT TGTGTTCTCA TAGAAATGGT CACTGGAAAG 
AGACCTTGGC ACGAGTACGA GCATAACTTC CAGATCATGT ATAGAGTGGG AATGGGCCAC 
AAACCGCCCA TTCCTGAAAA GCTGAGCACA GAGGGGAAGG ATTTTCTGGC TCACTGTCTG 
GAGAGCGAAC CCAAGCGGCG CTGGACCGCC AGTGCCCTGC TGGACCACCC TTTTGTCAAG 
GTCTGCACAG ATGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305771.1
CDS	669..5189
Translation	MEPPDEIRLKDSSKDDHQQITEVDESWDEPGQEEETLYCTSPPQTPRQIKRLSIKHQRSNPGSKGKEKSCSSSPLSQREREGARSAEPPEEHSYKQEKKQRFNQRSSERDVKKKFEGAFMLDPVSKSSTIGSRNMDPRKPYLSLGMIPVRTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKKKEKEQRGLENTAYMEQNNEVIWLELQAWHARRSVNDQDLYLFTARQHIPDIISEVLHFKVDYSSLCGLESGASVEESNSCPGETNCMDDPFSFSTCSASWQEIDAAAPFSSGLECRNHLQRQRVAFDQVKRVMSLLESVEALYPSLQTLQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMGTVLGLRDLSRIGWPIFEIPSPRCSRGNEDDEVDEDESESTTTFTAENDGEERTLSEATSDGEQSACSPPNFSRLMSEEEFLPRDNETCYCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRARTALLSHMPAQELPAVPGYSLQHSDYLPELSRHFSGPVEEEAESGRVSWQELMDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDHQGLQTNANIDEFEEDLHKMLEVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWECAEESTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEIAAEFSLTSGVSSLLQALKNKNYVKVQIPGLEELQVFVPCDLTEQRLVILQLLNAAAGKDCSKDPDEIPENEGYLLMSKHGVGDSNADGAWAEWDGTVLKLVPQMETVDTLRAMKVDNLLLVVMQSAHLVTQRKAFQQSMEELLSLSREQTSSQPLIVCALEQLKNEALQLCIKINDAIDRVEYMFMSEFEAEVEEAEKATLQQYYREAMIQGYNFAFEYHKEVVRLMSGEFRRRIGERYIAFARKWMNFVLTKCESGRGTRPRWATQGFDFLQAIEAAFISALPEDDFVSLQALMNECIGHVIGKPHSPVSSMYFAGPRNSPRPVKVPRCHSDPPNPYLFIPSTQAEGFRVSTGHENDRLSSVAAELQFKSLSRHSSPTEDREEPSYPKADPSSSARRSWELRNFISQSKDTARQSPLEAVRRSIQTFDEKHYALMRQRNIIGQVCNTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYRVGMGHKPPIPEKLSTEGKDFLAHCLESEPKRRWTASALLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_009307496.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307496.2

ORF Insert Sequence:

ATGGAGCCTC CGGATGAAAT CAGGCTCAAG GACTCGTCCA AAGATGATCA CCAGCAGATC 
ACTGAGGTGG ATGAGTCTTG GGACGAGCCC GGCCAGGAAG AGGAGACTCT CTACTGCACC 
TCCCCTCCCC AAACTCCTCG CCAGATAAAA CGTTTGTCCA TCAAACACCA GAGAAGCAAC 
CCGGGGTCAA AGGGCAAAGA GAAGTCGTGT TCATCCAGCC CCTTGTCTCA GCGTGAGCGT 
GAGGGAGCCA GATCAGCCGA ACCTCCTGAA GAGCACAGCT ACAAGCAGGA AAAGAAGCAG 
CGCTTCAACC AGCGATCCAG CGAGCGGGAC GTCAAGAAGA AGTTTGAAGG CGCCTTTATG 
CTGGATCCTG TTTCCAAGTC CAGTACCATC GGCTCCAGAA ACATGGATCC CCGCAAGCCC 
TACCTGAGTC TGGGCATGAT CCCCGTTCGC ACCCATCGGC AAACTTCGCG CACAGACTGT 
CCTGCAGACC GCCTTAAGTT CTTCGAGACC CTGCGGTTGC TGCTTAAACT CACCTCCATG 
TCATCCAAGA AGAAGGAGAA GGAGCAGCGA GGTCTGGAGA ACACCGCTTA TATGGAGCAG 
AACAACGAGG TCATCTGGCT GGAGCTGCAG GCCTGGCACG CTCGCCGTAG TGTTAATGAC 
CAGGACCTGT ACCTGTTCAC CGCTCGACAA CACATACCTG ACATCATCAG CGAGGTTTTG 
CACTTCAAAG TGGACTACAG TAGCCTGTGT GGACTCGAGA GTGGCGCCTC TGTTGAGGAG 
AGCAACTCCT GTCCTGGTGA AACTAATTGC ATGGACGATC CATTTAGCTT TAGCACATGT 
TCCGCGTCCT GGCAGGAAAT TGATGCCGCT GCTCCTTTTT CTTCAGGGCT GGAGTGCCGC 
AACCACCTGC AAAGGCAGCG AGTAGCTTTC GATCAGGTAA AACGGGTCAT GTCGCTGTTA 
GAGTCGGTGG AGGCTTTGTA CCCTTCTCTG CAAACCTTGC AGAAGGACTA TGAGAAGTAT 
GCAGCTCGGG ACTTCCAGGG CAGGGTGCAG GCGCTCTGCT TATGGCTCAA CATCACTCAG 
GACCTGAACC AGAAACTGCG CGTGATGGGC ACCGTGCTGG GCCTCAGGGA CCTTTCCCGC 
ATCGGCTGGC CCATCTTTGA GATTCCATCA CCTCGCTGCT CTCGAGGCAA TGAGGATGAT 
GAGGTGGATG AGGATGAATC AGAGTCCACC ACTACCTTTA CTGCCGAAAA CGATGGGGAA 
GAAAGGACTT TAAGTGAGGC AACCAGTGAC GGTGAGCAGT CGGCCTGCTC CCCGCCAAAT 
TTTTCACGCC TTATGTCAGA GGAGGAGTTC CTCCCTCGGG ACAACGAGAC CTGCTACTGT 
CCTACTGCCA TATACAGGCC GTTTGTGGAT AAGGCACTTA AACAGATGGG ACTGCGTAAA 
TTGATCCTCA GACTGCACAA GCTGATGGAC CGCTCTCTGC AGAGGGCCAG AACTGCTCTG 
TTGAGTCACA TGCCAGCTCA AGAGTTGCCA GCGGTCCCGG GTTATTCTTT GCAGCACTCT 
GACTACCTTC CAGAGCTTTC TCGTCACTTT TCCGGTCCAG TAGAAGAGGA GGCAGAGTCG 
GGACGGGTAT CATGGCAGGA GCTGATGGAC ATGGACCTGC CCTCATTCCG ACCAGCTTTC 
CTGGTGCTTT GCCGTGTGCT GCTAAACGTT ATTCACGAGT GCCTCAAACT GCGCCTTGAG 
CAAAGGCCTG CAGGAGAACC ATCCCTGCTC AGCATCAAAC AGTTGGTGCG CGAGTGTAAA 
GAGGTTCTTA AAGGAGGGCT CCTAATGAAA CAGTACTACC AGTTCATGCT GCGTGGCGTC 
GTCACTGACC ATCAGGGGCT GCAGACCAAT GCCAACATTG ACGAGTTTGA GGAGGATCTC 
CATAAAATGC TGGAGGTGTA CTTTGACTAC ATGCGCAGCT GGATCCAGAT GTTACAGCAG 
CTTCCTCAAG CATCCCACAG CCTGAAGAAC CTGCTGGAGG AGGAATGGAA CTTTACCAAA 
GTCATCACTC CATACATCCG AGGCGGAGAA GCCCAGTCTG GGAAGCTCTT CTGTGACATT 
GCTGGCATGC TGCTAAAGTC CACTGGAGAG TTTCTGGATG CTGGCCTTCA GAAGAGTGGC 
AATGAGTTCT GGGAGTGTGC AGAAGAGAGC ACCGCTTCAG ATGAGATCAG GCGTTCAGTG 
ATTGAGACGA GTCGTTCGCT CAAAGAGCTC TTTCATGAAG CTAGAGAAAG AGCATCTAAA 
GCGCTGGGCT TTGCCAAGAT GCTGCGTAAG GATCTGGAGA TTGCTGCTGA ATTCAGCTTG 
ACCAGTGGAG TTTCTTCTCT CCTCCAAGCA CTGAAGAACA AGAATTACGT CAAAGTCCAG 
ATCCCAGGAC TGGAGGAGCT GCAGGTGTTT GTCCCGTGTG ATCTGACGGA GCAGCGGCTG 
GTCATCTTGC AGCTGTTGAA TGCAGCAGCT GGAAAGGACT GCTCTAAAGA CCCAGATGAG 
ATCCCAGAAA ACGAGGGCTA TCTGCTGATG AGCAAACACG GGGTTGGGGA CTCGAACGCT 
GATGGAGCTT GGGCTGAATG GGACGGCACA GTGCTCAAAC TGGTGCCGCA GATGGAGACG 
GTGGACACAT TAAGGGCCAT GAAGGTGGAC AATCTGTTGC TGGTGGTGAT GCAGTCGGCT 
CATTTGGTAA CCCAACGCAA AGCTTTCCAG CAATCTATGG AGGAACTTCT CTCCCTCAGC 
CGAGAGCAGA CATCTAGCCA GCCGCTCATT GTCTGCGCTC TGGAGCAACT CAAGAATGAA 
GCTCTCCAAC TGTGCATCAA GATCAACGAT GCGATTGATC GTGTGGAGTA CATGTTCATG 
TCCGAGTTTG AGGCTGAGGT GGAGGAGGCG GAGAAGGCCA CGCTGCAGCA GTATTACAGA 
GAGGCCATGA TCCAGGGATA CAACTTTGCT TTCGAGTACC ATAAAGAGGT TGTGCGGTTG 
ATGTCCGGGG AGTTCAGACG AAGGATAGGA GAGCGTTACA TCGCTTTTGC CCGCAAGTGG 
ATGAACTTTG TCCTCACAAA GTGTGAAAGC GGAAGAGGCA CTCGTCCAAG GTGGGCGACG 
CAGGGCTTTG ATTTTCTTCA AGCGATCGAG GCTGCGTTTA TATCAGCCCT GCCAGAAGAT 
GATTTTGTGA GTCTTCAAGC TCTCATGAAT GAGTGTATTG GGCATGTTAT TGGCAAACCA 
CACAGTCCAG TCAGCAGTAT GTACTTTGCA GGCCCACGAA ACAGCCCTCG ACCTGTGAAA 
GTCCCCCGCT GTCACAGCGA TCCTCCAAAC CCGTACCTCT TCATCCCTTC CACCCAAGCA 
GAGGGTTTCA GAGTCTCCAC TGGACATGAG AATGACCGTC TGTCGTCTGT GGCTGCGGAG 
CTGCAGTTTA AATCCCTCAG TCGACACTCC AGCCCAACGG AGGACAGGGA AGAGCCGTCG 
TATCCCAAAG CAGACCCCAG CAGTTCAGCC CGTCGCAGCT GGGAGCTTCG CAACTTCATC 
AGCCAGTCTA AAGACACAGC GCGTCAGAGC CCCCTGGAGG CAGTGCGGCG CTCCATACAA 
ACCTTCGATG AGAAACACTA TGCGCTTATG AGACAGAGAA ACATCATTGG ACAGGTTTGC 
AACACGCCGA AGTCCTACGA TAACGTGATG CATGTGGGCC TGCGCAAAGT CACCTTTAAA 
TGGCAGAGAG GAAACAAGAT CGGTGAAGGG CAGTATGGGA AGGTTTACAC ATGCATCAAT 
GTGGACACTG GTGAACTAAT GGCCATGAAG GAGATTCGCT TTCAGCCTAA TGACCACAAA 
ACCATCAAGG AAACGGCCGA TGAGCTGAAG ATCTTCGAAG GAATCAAACA TCCCAACCTC 
GTCCGCTACT TCGGTGTTGA GTTGCACAGG GAGGAGATGT ACATCTTCAT GGAGTACTGT 
GATGAAGGCA CGCTGGAGGA AGTGTCCCGT CTGGGCCTCC AGGAACACGT CATTCGCCTC 
TACAGCAAAC AGATCACCAC GGCCATCAAT GTCCTGCATG AACACGGCAT CGTCCACCGA 
GACATTAAAG GAGCGAATAT CTTCCTCACG TCTTCGGGTC TGATAAAGCT GGGGGATTTT 
GGCTGCTCAG TGAAGCTGAA AAACAACGCT CAGACGATGC CTGGAGAGGT GAACAGCACT 
CTGGGAACAG CAGCATACAT GGCTCCTGAG GTGATCACCA GAGCGAAGGG TGAAGGTCAC 
GGTCGAGCTG CTGATATCTG GAGTCTCGGT TGTGTTCTCA TAGAAATGGT CACTGGAAAG 
AGACCTTGGC ACGAGTACGA GCATAACTTC CAGATCATGT ATAGAGTGGG AATGGGCCAC 
AAACCGCCCA TTCCTGAAAA GCTGAGCACA GAGGGGAAGG ATTTTCTGGC TCACTGTCTG 
GAGAGCGAAC CCAAGCGGCG CTGGACCGCC AGTGCCCTGC TGGACCACCC TTTTGTCAAG 
GTCTGCACAG ATGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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map3k4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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