kif20a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif20a
Entrez Gene ID	325449
Full Name	kinesin family member 20A
Synonyms	wu:fi34h02,wu:fj10c11,zgc:112061
General protein information	Preferred Names kinesin family member 20A Names kinesin-like protein KIF20A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001161470.1	NP_001154942.1	kinesin-like protein KIF20A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kif20a	NP_001101896.1
	KIF20A	XP_001107307.1
Bos taurus (cattle)	KIF20A	NP_001039753.1
Pan troglodytes (chimpanzee)	KIF20A	XP_001170694.1
Gallus gallus (chicken)	KIF20A	NP_001012801.1
Homo sapiens (human)	KIF20A	NP_005724.1
Mus musculus (house mouse)	Kif20a	NP_001159878.1
Xenopus tropicalis (tropical clawed frog)	kif20a	XP_002936881.1
Danio rerio (zebrafish)	LOC325449	NP_001154942.1

Gene Ontology
Bibliography

Related articles in PubMed

Compound heterozygous loss-of-function mutations in KIF20A are associated with a novel lethal congenital cardiomyopathy in two siblings.
Louw JJ, Nunes Bastos R, Chen X, Verdood C, Corveleyn A, Jia Y, Breckpot J, Gewillig M, Peeters H, Santoro MM, Barr F, Devriendt K
PLoS genetics14(1)e1007138(2018 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Dietary iron alters waterborne copper-induced gene expression in soft water acclimated zebrafish (Danio rerio).
Craig PM, Galus M, Wood CM, McClelland GB
American journal of physiology. Regulatory, integrative and comparative physiology296(2)R362-73(2009 Feb)

Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio).
Wu SM, Zheng YD, Kuo CH
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP148(2)184-93(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio).
Title: Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio).

The following kif20a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif20a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32613	NM_001161470.1 Latest version!	Danio rerio kinesin family member 20A (kif20a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif20a ( NM_001161470.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001154942.1 Danio rerio kinesin family member 20A (kif20a), mRNA.

Price & Availability↑

CloneID	ODa32613
Clone ID Related Accession (Same CDS sequence)	NM_001161470.1
Accession Version	NM_001161470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX957298.9 and BC095661.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC095661.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCTGG CGTCTCCATG CGGGATTCTG TCTGATGAGG AAGAGTGCGG GGCTGTGTTT 
GAGTCCACAG CGGCTGATGT TGTTGGTCTT ATGAGGTCTC AACGGTTTGT GTCTCCTCTG 
CCAGAGATCT CAGTCATTAC CCCTGCTGCA GATTCCCTAA CGCCTACTAA AGAGCTTGTG 
CAGATCAAAA GCGATATCAG CAGAAGAGGC AGCACTGATG ATGGATCAGA TAAACTTAAG 
GTTTTCCTAC GGATCCGTCC TCTTACAGAG GCAGAGAAGG ACCGGGGAGA AGAGCAGGGT 
TGTGTTAATG TGCAGTCTGA GGACAATCTG CTTCTCAAAG CACCTAAAGA CTCCCGCAAC 
ATGAAGAGTG CTGAGAGAGG GGTCGCTCAA AGTGTGCACA AATTTACATT TACAAAGATT 
TTTGGACCAC AAACCTCTCA GCAGGAGGTT TATGATCACA CCATTCGGGA GATGGTGCGA 
GATGTTCTAC GTGGAGAAAA CCGCCTTCTC TATACTTACG GTGTGACTAA CTCCGGCAAA 
ACCTACACCA TACAAGGGGC AGGTGCTGAG GCAGGTCTTC TGCCAAGGGC TTTAGTGTCT 
GTATTCCTGA AGCTGTCTGG TCGGCTGTAC ACTGCCATGG ACCTCAAGCC TGTTCTGAGC 
CAGGAAGTCC GTAAGCTAGA TGTTGGTGAG GTCCGTGCTG AGGAAATGCG TCGTGATGCT 
TTACTAAAAG AGGATGAAAG CAACCAGTCT AGGCTGCGTG CAGGCTTGTC CTGGGACAGT 
GGCATAAGTG GACTCTCAGC AACCAGTCAC ATAGCAACAC AGCTGGAAGA TTCAGATGGT 
GTTTGTCTGG AGGCCAATGG TCTGTGTCTC AGTGGTGGGG AGGATCTTGA AGAAGGGGTG 
CAGTTCTCAG TGTGGGTCTC GTTTTATGAG ATCTACAATG AATTTCTTTA TGATCTCCTG 
GACGCTCCAC CTTCCCTTCA GTCCCGCAAG AGAGTCACAC TACGCCTTAG TGATGACAAA 
CATGGAAACC CTTATGTGAA GGATTTGACC TGGATTCAGG TACACAGTGC AGAAGAAGCC 
TGGAAAGTGC TCAAGGTTGG ACAACGCAAT CAAAGTTTTG CCAGTACTCA CCTAAACCAC 
AACTCCAGTC GCAGTCATAG TATCTTTACA ATCCGTGTTC TACATGTCAA GCCACAACTG 
GGACAGGTGA CCAGAATCAG CGAACTCTCT GTATGTGATT TGGCTGGGTC TGAACGCTGT 
AAAGCACAGC AGAATGGTGA GAGAATGAAG GAGGCAAACA ACATCAACAC CTCTTTGCTG 
ACACTAGGCC GCTGCATCAC TGCTCTGAGA CACAATCAAA CCAACAAGTC TCGACCACCA 
GTGGTCGTTC CATTCAGAGA CAGCAAGTTA ACCAGGGTCC TCCAGAGTTT CTTTTGTGGT 
CATGGGCGCT CTTGCATGGT GGTGAACATC AACCCTTGTG CCTCCACCTA TGATGAGACA 
CTCCAAGCAC TCAAATTCTC TGCAATTGCC ACACAGCTGG TGCATGGCCC GTGCAGTAAG 
ACACGTGTTG CATACATCCT GTCCCTGCTC CGTGAGCAGC CAACTCATCC CAACGACACC 
ACTTTACTAG AGGAGGATGA GGAGGAGAGT GATGAGGAAG GAGACATCAC CATGTTTGAT 
CCTGAGGCAC TGCTGAAGGC CATTGATGTT CTAAAGCGTG AGGTGCTGCG CCAACGGCAA 
GAAAAAGAGG AACTGGAGGC TAAGGTTCGA GAGGAGGTCT GCACTGAGAT GATGGAGGTT 
ATCAGCCGCA TGCAGGATGA CTTCAGTGAG ACCCTGGAGA GCGAGAGGGA TCTGATGGAG 
AAGAGATGTG AGAACAAAAT TAACAACCTG AAGAGCAGCT TGAAGAAATA TTACAGCCAA 
GAGCTAGAGG AGCGAGATGA TGAGATCAGA GAACTTACTG CTGCTCTTAA GGAAAAGGAA 
GCAGGTAATA CTGCCCCGGC TGCCATTTTC CCACCTCTGA ATGATGACGG TGGTCTGCGT 
CGTTCACAAC GCTTGGCTTC AACTCACAAA CGAGATGCAG ATCATGAGAG TATAGAGCTC 
TTTCGGTGTA AAGCAGAGCT GGAAAATTGC AGAGCCGAAC TAGACCTGAA GCAAACAGAA 
CTGCGCCTCA AAACACTTGA ATTGAAGCGT TTCCAGGACA ACCTCCCATC GATGGGCGTT 
ACGGGAACCC TGACAACTAC AGCTGAGCGG AAACTGGAGG AGGGTCAGAG GAACCTGAAG 
CAGCTGCGGA TGGAGCTTCA GTTGCTGGGC ATTAAGCTGC AGTCAGGAGA AAGAGCCTGC 
TGCCGCAACA CAGATGGAGA AAAGCTTCGT CACGCTCTCT CTTCAGCAGA CAGTAAACTC 
GCCAAACAGG ACCAAATGCT AGTCGAGCTG CACACGAGCC TGCAGCTGGT CAAAGCGGAA 
CTGCGTAAGA AAGACGAGCT CCTGACGCGC TTCCAGAACA CTCAACAAGC CCAGTCCCAC 
CAGCCTCCAA CGGCATCCGG CTCCTGCAAG AAAAGGGGTT GCGGGGTAGC GGTAGCTCTG 
GCGGAGAACC AGCCTCCAGA AAAACGCCCT TTCTTCCGTT CGCTGTTTCC CTCCCGCACG 
CCCTCAAAGA CCCCCTCACG ACCAACCCGA CAGCAAAATG AGGTCACCAC ACCTTATTCT 
CGTGTGTTGC GTTCACGACA GCCATCGCCT CCTACCACTC CCGTCATGCT CCGAGTCAAG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154942.1
CDS	185..2950
Translation	MALASPCGILSDEEECGAVFESTAADVVGLMRSQRFVSPLPEISVITPAADSLTPTKELVQIKSDISRRGSTDDGSDKLKVFLRIRPLTEAEKDRGEEQGCVNVQSEDNLLLKAPKDSRNMKSAERGVAQSVHKFTFTKIFGPQTSQQEVYDHTIREMVRDVLRGENRLLYTYGVTNSGKTYTIQGAGAEAGLLPRALVSVFLKLSGRLYTAMDLKPVLSQEVRKLDVGEVRAEEMRRDALLKEDESNQSRLRAGLSWDSGISGLSATSHIATQLEDSDGVCLEANGLCLSGGEDLEEGVQFSVWVSFYEIYNEFLYDLLDAPPSLQSRKRVTLRLSDDKHGNPYVKDLTWIQVHSAEEAWKVLKVGQRNQSFASTHLNHNSSRSHSIFTIRVLHVKPQLGQVTRISELSVCDLAGSERCKAQQNGERMKEANNINTSLLTLGRCITALRHNQTNKSRPPVVVPFRDSKLTRVLQSFFCGHGRSCMVVNINPCASTYDETLQALKFSAIATQLVHGPCSKTRVAYILSLLREQPTHPNDTTLLEEDEEESDEEGDITMFDPEALLKAIDVLKREVLRQRQEKEELEAKVREEVCTEMMEVISRMQDDFSETLESERDLMEKRCENKINNLKSSLKKYYSQELEERDDEIRELTAALKEKEAGNTAPAAIFPPLNDDGGLRRSQRLASTHKRDADHESIELFRCKAELENCRAELDLKQTELRLKTLELKRFQDNLPSMGVTGTLTTTAERKLEEGQRNLKQLRMELQLLGIKLQSGERACCRNTDGEKLRHALSSADSKLAKQDQMLVELHTSLQLVKAELRKKDELLTRFQNTQQAQSHQPPTASGSCKKRGCGVAVALAENQPPEKRPFFRSLFPSRTPSKTPSRPTRQQNEVTTPYSRVLRSRQPSPPTTPVMLRVKY

Target ORF information:

RefSeq Version	NM_001161470.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20A (kif20a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161470.1

ORF Insert Sequence:

ATGGCTCTGG CGTCTCCATG CGGGATTCTG TCTGATGAGG AAGAGTGCGG GGCTGTGTTT 
GAGTCCACAG CGGCTGATGT TGTTGGTCTT ATGAGGTCTC AACGGTTTGT GTCTCCTCTG 
CCAGAGATCT CAGTCATTAC CCCTGCTGCA GATTCCCTAA CGCCTACTAA AGAGCTTGTG 
CAGATCAAAA GCGATATCAG CAGAAGAGGC AGCACTGATG ATGGATCAGA TAAACTTAAG 
GTTTTCCTAC GGATCCGTCC TCTTACAGAG GCAGAGAAGG ACCGGGGAGA AGAGCAGGGT 
TGTGTTAATG TGCAGTCTGA GGACAATCTG CTTCTCAAAG CACCTAAAGA CTCCCGCAAC 
ATGAAGAGTG CTGAGAGAGG GGTCGCTCAA AGTGTGCACA AATTTACATT TACAAAGATT 
TTTGGACCAC AAACCTCTCA GCAGGAGGTT TATGATCACA CCATTCGGGA GATGGTGCGA 
GATGTTCTAC GTGGAGAAAA CCGCCTTCTC TATACTTACG GTGTGACTAA CTCCGGCAAA 
ACCTACACCA TACAAGGGGC AGGTGCTGAG GCAGGTCTTC TGCCAAGGGC TTTAGTGTCT 
GTATTCCTGA AGCTGTCTGG TCGGCTGTAC ACTGCCATGG ACCTCAAGCC TGTTCTGAGC 
CAGGAAGTCC GTAAGCTAGA TGTTGGTGAG GTCCGTGCTG AGGAAATGCG TCGTGATGCT 
TTACTAAAAG AGGATGAAAG CAACCAGTCT AGGCTGCGTG CAGGCTTGTC CTGGGACAGT 
GGCATAAGTG GACTCTCAGC AACCAGTCAC ATAGCAACAC AGCTGGAAGA TTCAGATGGT 
GTTTGTCTGG AGGCCAATGG TCTGTGTCTC AGTGGTGGGG AGGATCTTGA AGAAGGGGTG 
CAGTTCTCAG TGTGGGTCTC GTTTTATGAG ATCTACAATG AATTTCTTTA TGATCTCCTG 
GACGCTCCAC CTTCCCTTCA GTCCCGCAAG AGAGTCACAC TACGCCTTAG TGATGACAAA 
CATGGAAACC CTTATGTGAA GGATTTGACC TGGATTCAGG TACACAGTGC AGAAGAAGCC 
TGGAAAGTGC TCAAGGTTGG ACAACGCAAT CAAAGTTTTG CCAGTACTCA CCTAAACCAC 
AACTCCAGTC GCAGTCATAG TATCTTTACA ATCCGTGTTC TACATGTCAA GCCACAACTG 
GGACAGGTGA CCAGAATCAG CGAACTCTCT GTATGTGATT TGGCTGGGTC TGAACGCTGT 
AAAGCACAGC AGAATGGTGA GAGAATGAAG GAGGCAAACA ACATCAACAC CTCTTTGCTG 
ACACTAGGCC GCTGCATCAC TGCTCTGAGA CACAATCAAA CCAACAAGTC TCGACCACCA 
GTGGTCGTTC CATTCAGAGA CAGCAAGTTA ACCAGGGTCC TCCAGAGTTT CTTTTGTGGT 
CATGGGCGCT CTTGCATGGT GGTGAACATC AACCCTTGTG CCTCCACCTA TGATGAGACA 
CTCCAAGCAC TCAAATTCTC TGCAATTGCC ACACAGCTGG TGCATGGCCC GTGCAGTAAG 
ACACGTGTTG CATACATCCT GTCCCTGCTC CGTGAGCAGC CAACTCATCC CAACGACACC 
ACTTTACTAG AGGAGGATGA GGAGGAGAGT GATGAGGAAG GAGACATCAC CATGTTTGAT 
CCTGAGGCAC TGCTGAAGGC CATTGATGTT CTAAAGCGTG AGGTGCTGCG CCAACGGCAA 
GAAAAAGAGG AACTGGAGGC TAAGGTTCGA GAGGAGGTCT GCACTGAGAT GATGGAGGTT 
ATCAGCCGCA TGCAGGATGA CTTCAGTGAG ACCCTGGAGA GCGAGAGGGA TCTGATGGAG 
AAGAGATGTG AGAACAAAAT TAACAACCTG AAGAGCAGCT TGAAGAAATA TTACAGCCAA 
GAGCTAGAGG AGCGAGATGA TGAGATCAGA GAACTTACTG CTGCTCTTAA GGAAAAGGAA 
GCAGGTAATA CTGCCCCGGC TGCCATTTTC CCACCTCTGA ATGATGACGG TGGTCTGCGT 
CGTTCACAAC GCTTGGCTTC AACTCACAAA CGAGATGCAG ATCATGAGAG TATAGAGCTC 
TTTCGGTGTA AAGCAGAGCT GGAAAATTGC AGAGCCGAAC TAGACCTGAA GCAAACAGAA 
CTGCGCCTCA AAACACTTGA ATTGAAGCGT TTCCAGGACA ACCTCCCATC GATGGGCGTT 
ACGGGAACCC TGACAACTAC AGCTGAGCGG AAACTGGAGG AGGGTCAGAG GAACCTGAAG 
CAGCTGCGGA TGGAGCTTCA GTTGCTGGGC ATTAAGCTGC AGTCAGGAGA AAGAGCCTGC 
TGCCGCAACA CAGATGGAGA AAAGCTTCGT CACGCTCTCT CTTCAGCAGA CAGTAAACTC 
GCCAAACAGG ACCAAATGCT AGTCGAGCTG CACACGAGCC TGCAGCTGGT CAAAGCGGAA 
CTGCGTAAGA AAGACGAGCT CCTGACGCGC TTCCAGAACA CTCAACAAGC CCAGTCCCAC 
CAGCCTCCAA CGGCATCCGG CTCCTGCAAG AAAAGGGGTT GCGGGGTAGC GGTAGCTCTG 
GCGGAGAACC AGCCTCCAGA AAAACGCCCT TTCTTCCGTT CGCTGTTTCC CTCCCGCACG 
CCCTCAAAGA CCCCCTCACG ACCAACCCGA CAGCAAAATG AGGTCACCAC ACCTTATTCT 
CGTGTGTTGC GTTCACGACA GCCATCGCCT CCTACCACTC CCGTCATGCT CCGAGTCAAG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compound heterozygous loss-of-function mutations in KIF20A are associated with a novel lethal congenital cardiomyopathy in two siblings.
PLoS genetics14(1)e1007138(2018 Jan)
Louw JJ,Nunes Bastos R,Chen X,Verdood C,Corveleyn A,Jia Y,Breckpot J,Gewillig M,Peeters H,Santoro MM,Barr F,Devriendt K

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Dietary iron alters waterborne copper-induced gene expression in soft water acclimated zebrafish (Danio rerio).
American journal of physiology. Regulatory, integrative and comparative physiology296(2)R362-73(2009 Feb)
Craig PM,Galus M,Wood CM,McClelland GB

Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio).
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP148(2)184-93(2008 Aug)
Wu SM,Zheng YD,Kuo CH

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kif20a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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