mkln1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkln1
Entrez Gene ID	325217
Full Name	muskelin 1, intracellular mediator containing kelch motifs
Synonyms	fc60a07,wu:fc60a07
General protein information	Preferred Names muskelin 1, intracellular mediator containing kelch motifs Names muskelin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212694.1	NP_997859.1	muskelin

Pathways from BioSystems

Homologies

Homo sapiens (human)	MKLN1	NP_037387.2
Canis lupus familiaris (dog)	MKLN1	XP_005628424.1
Schizosaccharomyces pombe (fission yeast)	mde6	NP_594297.2
	MKLN1	XP_001098817.1
Bos taurus (cattle)	MKLN1	NP_001091468.1
Gallus gallus (chicken)	MKLN1	NP_001025944.1
Danio rerio (zebrafish)	mkln1	NP_997859.1
Drosophila melanogaster (fruit fly)	muskelin	NP_610801.1
Rattus norvegicus (Norway rat)	Mkln1	NP_112649.1
Mus musculus (house mouse)	Mkln1	NP_038819.1
Pan troglodytes (chimpanzee)	MKLN1	XP_519385.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004884	XP_314298.3
Xenopus tropicalis (tropical clawed frog)	mkln1	XP_002931895.1

Gene Ontology
Bibliography

Related articles in PubMed

Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish.
Mishima Y, Tomari Y
Genes to cells : devoted to molecular & cellular mechanisms22(7)670-678(2017 Jul)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Molecular analysis of muskelin identifies a conserved discoidin-like domain that contributes to protein self-association.
Prag S, Collett GD, Adams JC
The Biochemical journal381(Pt 2)547-59(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mkln1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkln1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31615	NM_212694.1 Latest version!	Danio rerio muskelin 1, intracellular mediator containing kelch motifs (mkln1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkln1 ( NM_212694.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997859.1 Danio rerio muskelin 1, intracellular mediator containing kelch motifs (mkln1), mRNA.

Price & Availability↑

CloneID	ODa31615
Clone ID Related Accession (Same CDS sequence)	NM_212694.1
Accession Version	NM_212694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	muskelin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF418017.1. ##Evidence-Data-START## Transcript exon combination :: AF418017.1, BC165655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTGG CTCCTGACAG CAGAGTGCTT TCCTACAGTG TTTATAAATG GAGCTCGTAC 
TCCTCTACAT ATTTACCCGA GAATATTTTA GTGGATAAAC CTAATGATCA GTCTTCCAGG 
TGGTCATCTG AGAGCAACTA CCCTCCACAG TACTTGATCC TAAAACTGGA GAGACCAGCT 
ATAGTTCTGA GCATCACCTA TGGCAAATAT GAGAAAACCC ACGTCTGCAA CCTGAAGAAG 
TTTAAGGTGT TTGGAGGCAT GAGTGAGGAA AACATGACGG AGCTCTTGTC TAGTGGCCTT 
AAGAATGACT TCAACAAGGA GACGTTCACG CTAAAGCACA AGATCGATGA GCAAATGTTT 
CCCTGCAGGT TCATTAAAAT AGTGCCTCTG ATGTCATGGG GGCCCAGTTT TAACTTCAGC 
ATTTGGTTTA TTGAGTTACA CGGCATTGAA GATCCAGAGG TTGTCCAGCC CTGCCTTAAC 
TGGTACAGTA AGTACCGTGA ACAAGAGGCC ATCCGCTTGT GCCTGAAACA CTTCCGGCAG 
CACAACTACA CAGAAGCCTT CGAATCCCTG CAGAAGAAAA CACGGATCGC TCTGGAGCAC 
CCCATGCTTA CGCACCTACA CGAGCGGCTG GTTCTCAGAG GAGACTTTGA CGCCTGCGAG 
GAGCTGATAG ATAAGGCCGT GAGAGATGGT TTGTTTAATC AGTACATCAG CCAGCAGGAT 
TATAAGCCAC GCTGGAGTCA GATCATCCCC AAATGTAACA AAGGCGATGG TGATGATAAC 
AGGCCGGGCA TGAGAGGAGG TCATCAAATG GTCATTGATG TTCAGACAGA AACCGTGTAT 
TTGTTCGGTG GCTGGGATGG AACGCAGGAC TTGGCTGATT TCTGGGCGTA CAGTGTGAAG 
GAGAACCAAT GGGCCTGCAT CTCCAGGGAC ACAGAGAAAG AGAGTGGTCC CAGTGCTCGT 
TCATGCCACA AAATGTGCAT TGATTCTCAA CGGAGGCAGA TCTACACGCT GGGCCGATAT 
CTGGACTCCT CTGTCAGAAA CAGCAAGTCT CTGAAGAGTG ACTTCTACTG CTACGACATT 
GACGCAAACA CGTGGACCCT CCTGAGTGAA GACACATCCG CCGATGGAGG ACCCAAACTT 
GTGTTCGACC ACCAGATGTG CATGGACTCT GAGAAGCACA TGATCTACAC GTTTGGTGGT 
CGGATACTGA CGTGTAATGG CAGCGTGGAT GATGGCAGGA CGTCTGAACC TCAGTTCAGT 
GGGCTTTACG CGTATCATTG TCAAGCTGGC AGCTGGAGCC TACTCCGAGA AGACTCGTGT 
AACGCAGGGC CGGAGGACAT CCAATCTCGC ATCGGACACT GCATGCTCTT CCACACGAGA 
AATCGTTGCC TGTATGTTTT TGGAGGTCAG AGGTCCAAAA CGTACTTGAA TGATTTTTTC 
AGTTACGATG TTGACGGTGA CCATGTGGAG ATCATCTCAG ATGGCACCAA GAAGGACTCT 
GGCATGGTAC CAATGACGGG CTTCACTCAG CGTGCCACTA TTGACCCAGA GCTCAACGAG 
ATTCACGTCC TGTCGGGACT CAGCAAAGAC AAAGATAAAC GTGAGGAGAA CGTGCGCAAC 
TCCTTCTGGA TTTATGACAT CGCACGCAAC AACTGGTCCT GTGTTTATAA GAACGACCAA 
GCGGTGAAGG AAAACCCCAC TAAGGCCCTG CAGGAAGAGG AACCTTGTCC TCGGTTTGCT 
CATCAGCTTG TCTACGATGA ACTCCACAAG GTGCATTACC TGTTTGGTGG AAACCCAGGA 
AAGTCCAGCT CTCCCAAAAT GCGACTGGAT GACTTCTGGT CCCTAAAGCT CTGCCGCCCT 
TCCAAAGAAT ACCTGCTGCG ACACTGCAAA TACCTCATTC GCAAATACAG GTTTGAGGAA 
AAGGCTCAGA CCGAGCCCTT GAATGCACTT AAATATCTAC AGAATGATCT GTCCCTCACT 
GTGGACCACA CTGATCCTGA TGAGACGAAA GAGTTCCAGC TCTTGCCCTC CGCTCTGTTC 
AAATCCAGCT CAGACTTCAT TCCTCTAGGC TTTTCAGATG TGGATCAGAC ATACGCCCAG 
CGCACACAGC TGTTCGACAC ACTCGTCAAT TTCTTCCCCG ACAATATGAC CCCGCCCAAG 
GGTAACCTCG TCGACCTCAT CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997859.1
CDS	32..2221
Translation	MAVAPDSRVLSYSVYKWSSYSSTYLPENILVDKPNDQSSRWSSESNYPPQYLILKLERPAIVLSITYGKYEKTHVCNLKKFKVFGGMSEENMTELLSSGLKNDFNKETFTLKHKIDEQMFPCRFIKIVPLMSWGPSFNFSIWFIELHGIEDPEVVQPCLNWYSKYREQEAIRLCLKHFRQHNYTEAFESLQKKTRIALEHPMLTHLHERLVLRGDFDACEELIDKAVRDGLFNQYISQQDYKPRWSQIIPKCNKGDGDDNRPGMRGGHQMVIDVQTETVYLFGGWDGTQDLADFWAYSVKENQWACISRDTEKESGPSARSCHKMCIDSQRRQIYTLGRYLDSSVRNSKSLKSDFYCYDIDANTWTLLSEDTSADGGPKLVFDHQMCMDSEKHMIYTFGGRILTCNGSVDDGRTSEPQFSGLYAYHCQAGSWSLLREDSCNAGPEDIQSRIGHCMLFHTRNRCLYVFGGQRSKTYLNDFFSYDVDGDHVEIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKDKREENVRNSFWIYDIARNNWSCVYKNDQAVKENPTKALQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSSSPKMRLDDFWSLKLCRPSKEYLLRHCKYLIRKYRFEEKAQTEPLNALKYLQNDLSLTVDHTDPDETKEFQLLPSALFKSSSDFIPLGFSDVDQTYAQRTQLFDTLVNFFPDNMTPPKGNLVDLITL

Target ORF information:

RefSeq Version	NM_212694.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio muskelin 1, intracellular mediator containing kelch motifs (mkln1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212694.1

ORF Insert Sequence:

ATGGCGGTGG CTCCTGACAG CAGAGTGCTT TCCTACAGTG TTTATAAATG GAGCTCGTAC 
TCCTCTACAT ATTTACCCGA GAATATTTTA GTGGATAAAC CTAATGATCA GTCTTCCAGG 
TGGTCATCTG AGAGCAACTA CCCTCCACAG TACTTGATCC TAAAACTGGA GAGACCAGCT 
ATAGTTCTGA GCATCACCTA TGGCAAATAT GAGAAAACCC ACGTCTGCAA CCTGAAGAAG 
TTTAAGGTGT TTGGAGGCAT GAGTGAGGAA AACATGACGG AGCTCTTGTC TAGTGGCCTT 
AAGAATGACT TCAACAAGGA GACGTTCACG CTAAAGCACA AGATCGATGA GCAAATGTTT 
CCCTGCAGGT TCATTAAAAT AGTGCCTCTG ATGTCATGGG GGCCCAGTTT TAACTTCAGC 
ATTTGGTTTA TTGAGTTACA CGGCATTGAA GATCCAGAGG TTGTCCAGCC CTGCCTTAAC 
TGGTACAGTA AGTACCGTGA ACAAGAGGCC ATCCGCTTGT GCCTGAAACA CTTCCGGCAG 
CACAACTACA CAGAAGCCTT CGAATCCCTG CAGAAGAAAA CACGGATCGC TCTGGAGCAC 
CCCATGCTTA CGCACCTACA CGAGCGGCTG GTTCTCAGAG GAGACTTTGA CGCCTGCGAG 
GAGCTGATAG ATAAGGCCGT GAGAGATGGT TTGTTTAATC AGTACATCAG CCAGCAGGAT 
TATAAGCCAC GCTGGAGTCA GATCATCCCC AAATGTAACA AAGGCGATGG TGATGATAAC 
AGGCCGGGCA TGAGAGGAGG TCATCAAATG GTCATTGATG TTCAGACAGA AACCGTGTAT 
TTGTTCGGTG GCTGGGATGG AACGCAGGAC TTGGCTGATT TCTGGGCGTA CAGTGTGAAG 
GAGAACCAAT GGGCCTGCAT CTCCAGGGAC ACAGAGAAAG AGAGTGGTCC CAGTGCTCGT 
TCATGCCACA AAATGTGCAT TGATTCTCAA CGGAGGCAGA TCTACACGCT GGGCCGATAT 
CTGGACTCCT CTGTCAGAAA CAGCAAGTCT CTGAAGAGTG ACTTCTACTG CTACGACATT 
GACGCAAACA CGTGGACCCT CCTGAGTGAA GACACATCCG CCGATGGAGG ACCCAAACTT 
GTGTTCGACC ACCAGATGTG CATGGACTCT GAGAAGCACA TGATCTACAC GTTTGGTGGT 
CGGATACTGA CGTGTAATGG CAGCGTGGAT GATGGCAGGA CGTCTGAACC TCAGTTCAGT 
GGGCTTTACG CGTATCATTG TCAAGCTGGC AGCTGGAGCC TACTCCGAGA AGACTCGTGT 
AACGCAGGGC CGGAGGACAT CCAATCTCGC ATCGGACACT GCATGCTCTT CCACACGAGA 
AATCGTTGCC TGTATGTTTT TGGAGGTCAG AGGTCCAAAA CGTACTTGAA TGATTTTTTC 
AGTTACGATG TTGACGGTGA CCATGTGGAG ATCATCTCAG ATGGCACCAA GAAGGACTCT 
GGCATGGTAC CAATGACGGG CTTCACTCAG CGTGCCACTA TTGACCCAGA GCTCAACGAG 
ATTCACGTCC TGTCGGGACT CAGCAAAGAC AAAGATAAAC GTGAGGAGAA CGTGCGCAAC 
TCCTTCTGGA TTTATGACAT CGCACGCAAC AACTGGTCCT GTGTTTATAA GAACGACCAA 
GCGGTGAAGG AAAACCCCAC TAAGGCCCTG CAGGAAGAGG AACCTTGTCC TCGGTTTGCT 
CATCAGCTTG TCTACGATGA ACTCCACAAG GTGCATTACC TGTTTGGTGG AAACCCAGGA 
AAGTCCAGCT CTCCCAAAAT GCGACTGGAT GACTTCTGGT CCCTAAAGCT CTGCCGCCCT 
TCCAAAGAAT ACCTGCTGCG ACACTGCAAA TACCTCATTC GCAAATACAG GTTTGAGGAA 
AAGGCTCAGA CCGAGCCCTT GAATGCACTT AAATATCTAC AGAATGATCT GTCCCTCACT 
GTGGACCACA CTGATCCTGA TGAGACGAAA GAGTTCCAGC TCTTGCCCTC CGCTCTGTTC 
AAATCCAGCT CAGACTTCAT TCCTCTAGGC TTTTCAGATG TGGATCAGAC ATACGCCCAG 
CGCACACAGC TGTTCGACAC ACTCGTCAAT TTCTTCCCCG ACAATATGAC CCCGCCCAAG 
GGTAACCTCG TCGACCTCAT CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish.
Genes to cells : devoted to molecular & cellular mechanisms22(7)670-678(2017 Jul)
Mishima Y,Tomari Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Molecular analysis of muskelin identifies a conserved discoidin-like domain that contributes to protein self-association.
The Biochemical journal381(Pt 2)547-59(2004 Jul)
Prag S,Collett GD,Adams JC

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mkln1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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